Md Murshed Hasan Sarkar, Showti Raheel Naser, Sanjana Fatema Chowdhury, Md Salim Khan, Md Ahashan Habib, Shahina Akter, Tanjina Akhtar Banu, Barna Goswami, Iffat Jahan, Maksudur Rahman Nayem, Md Akibul Hassan, Md Imran Khan, Mohammad Fazle Alam Rabbi, Chowdhury Rafiqul Ahsan, Md Ibrahim Miah, Afzalun Nessa, S M Rashed Ul Islam, Mohammed Atiqur Rahman, Md Aftab Ali Shaikh, Md Sharfuddin Ahmed
Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) is the gold standard method for SARS-CoV-2 detection, and several qRT-PCR kits have been established targeting different genes of the virus. Due to the high mutation rate of these genes, false negative results arise thus complicating the interpretation of the diagnosis and increasing the need of alternative targets. In this study, an alternative approach for the detection of SARS-CoV-2 viral RNA targeting the membrane (M) gene of the virus using qRT-PCR was described...
October 3, 2023: Scientific Reports