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https://www.readbyqxmd.com/read/28211112/impact-of-a-targeted-direct-marketing-price-promotion-intervention-buywell-on-food-purchasing-behaviour-by-low-income-consumers-a-randomised-controlled-trial
#1
M Stead, A M MacKintosh, A Findlay, L Sparks, A S Anderson, K Barton, D Eadie
BACKGROUND: Price promotions are a promising intervention for encouraging healthier food purchasing. We aimed to assess the impact of a targeted direct marketing price promotion combined with healthy eating advice and recipe suggestions on the purchase of selected healthier foods by low income consumers. METHODS: We conducted a randomised controlled trial (n = 53 367) of a direct marketing price promotion (Buywell) combined with healthy eating advice and recipe suggestions for low income consumers identified as 'less healthy' shoppers...
February 17, 2017: Journal of Human Nutrition and Dietetics: the Official Journal of the British Dietetic Association
https://www.readbyqxmd.com/read/28208254/electrochemical-detection-of-escherichia-coli-from-aqueous-samples-using-engineered-phages
#2
Danhui Wang, Juhong Chen, Sam R Nugen
In this study, an enzyme-based electrochemical method was developed for the detection of Escherichia coli (E. coli) using the T7 bacteriophages engineered with lacZ operon encoding for beta-galactosidase (β-gal). The T7lacZ phages can infect E. coli, and have the ability to trigger the overexpression of β-gal during the infection of E. coli. The use of the engineered phages resulted in a more sensitive detection of E. coli by (1) overexpression of β-gal in E. coli during the specific infection and (2) release of the endogenous intracellular β-gal from E...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28202335/purification-and-characterization-of-a-milk-clotting-aspartic-protease-from-withania-coagulans-fruit
#3
Mahmoud Salehi, Mahmoud Reza Aghamaali, Reza H Sajedi, S Mohsen Asghari, Eisa Jorjani
Withania coagulans fruit has traditionally been used as milk coagulant. The present study reports the purification and characterization of an aspartic protease from W. coagulans fruit. The enzyme was purified via fractional ammonium sulfate precipitation and cation exchange chromatography. SDS-PAGE analysis revealed the presence of a monomeric protein with molecular weight of 31kDa. Proteolytic activity (PA) of the protease was evaluated using casein, and the milk-clotting activity (MCA) was analyzed by skim milk...
February 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28099518/evaluation-of-methods-for-the-concentration-and-extraction-of-viruses-from-sewage-in-the-context-of-metagenomic-sequencing
#4
Mathis Hjort Hjelmsø, Maria Hellmér, Xavier Fernandez-Cassi, Natàlia Timoneda, Oksana Lukjancenko, Michael Seidel, Dennis Elsässer, Frank M Aarestrup, Charlotta Löfström, Sílvia Bofill-Mas, Josep F Abril, Rosina Girones, Anna Charlotte Schultz
Viral sewage metagenomics is a novel field of study used for surveillance, epidemiological studies, and evaluation of waste water treatment efficiency. In raw sewage human waste is mixed with household, industrial and drainage water, and virus particles are, therefore, only found in low concentrations. This necessitates a step of sample concentration to allow for sensitive virus detection. Additionally, viruses harbor a large diversity of both surface and genome structures, which makes universal viral genomic extraction difficult...
2017: PloS One
https://www.readbyqxmd.com/read/28099471/molybdate-in-rhizobial-seed-coat-formulations-improves-the-production-and-nodulation-of-alfalfa
#5
Jiqiong Zhou, Bo Deng, Yingjun Zhang, Adam B Cobb, Zhao Zhang
Rhizobia-legume symbiosis is the most well researched biological nitrogen fixation system. Coating legume seeds with rhizobia is now a recognized practical measure for improving the production of legume corp. However, the efficacy of some commercial rhizobia inoculants cannot be guaranteed in China due to the low rate of live rhizobia in these products. A greenhouse experiment was conducted to assess the effects of different rhizobial inoculant formulations on alfalfa productivity and nitrogen fixation. Two rhizobia strains, (ACCC17631 and ACCC17676), that are effective partners with alfalfa variety Zhongmu No...
2017: PloS One
https://www.readbyqxmd.com/read/28094048/optimization-of-the-skimmed-milk-flocculation-method-for-recovery-of-adenovirus-from-sludge
#6
Andrêssa S F Assis, Marcelo Henrique Otenio, Betânia P Drumond, Tulio M Fumian, Marize P Miagostovich, Maria Luzia da Rosa E Silva
Return of treated sludge to the environment poses concerns and has stimulated the development of studies on viral monitoring in this matrix, in order to assess its potential risks for public health. Human adenovirus (HAdV) has been identified as a putative viral marker of faecal contamination due to its stability and resistance to the sewage treatment process. The aim of this study was to optimize the organic flocculation procedure in order to establish an appropriate methodology for HAdV recovery from sewage sludge samples...
January 13, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28093213/characterization-of-the-efficiency-and-uncertainty-of-skimmed-milk-flocculation-for-the-simultaneous-concentration-and-quantification-of-water-borne-viruses-bacteria-and-protozoa
#7
Eloy Gonzales-Gustavson, Yexenia Cárdenas-Youngs, Miquel Calvo, Marcelle Figueira Marques da Silva, Ayalkibet Hundesa, Inmaculada Amorós, Yolanda Moreno, Laura Moreno-Mesonero, Rosa Rosell, Llilianne Ganges, Rosa Araujo, Rosina Girones
In this study, the use of skimmed milk flocculation (SMF) to simultaneously concentrate viruses, bacteria and protozoa was evaluated. We selected strains of faecal indicator bacteria and pathogens, such as Escherichia coli and Helicobacter pylori. The viruses selected were adenovirus (HAdV 35), rotavirus (RoV SA-11), the bacteriophage MS2 and bovine viral diarrhoea virus (BVDV). The protozoa tested were Acanthamoeba, Giardia and Cryptosporidium. The mean recoveries with q(RT)PCR were 66% (HAdV 35), 24% (MS2), 28% (RoV SA-11), 15% (BVDV), 60% (E...
January 16, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28092156/inhibition-of-cronobacter-sakazakii-adhesion-to-caco-2-cells-by-commercial-dairy-powders-and-raw-buttermilk
#8
Daniel Ripollés, Saidou Harouna, José A Parrón, Irene Arenales, Miguel Calvo, María D Pérez, Lourdes Sánchez
Cronobacter sakazakii is a foodborne pathogen that has been associated with severe infections, mainly in neonates. The binding of this bacterium to host cell surfaces represents the first step in the pathogenesis of disease. An ELISA-based assay has been developed using a polyclonal antiserum against C. sakazakii to determine its adhesion to Caco-2 cells. The antiserum used recognized many of the outer membrane proteins of C. sakazakii. A positive correlation was found between the absorbance values obtained by ELISA and the number of bacteria adhered to cells determined by plate counting...
January 30, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28091716/rapid-concentration-detection-and-differentiation-of-bacteria-in-skimmed-milk-using-surface-enhanced-raman-scattering-mapping-on-4-mercaptophenylboronic-acid-functionalized-silver-dendrites
#9
Panxue Wang, Shintaro Pang, Brooke Pearson, Yayoi Chujo, Lynne McLandsborough, Mingtao Fan, Lili He
A novel method was developed to rapidly concentrate, detect, and differentiate bacteria in skimmed milk using surface enhanced Raman scattering (SERS) mapping on 4-mercaptophenylboronic acid (4-MPBA) functionalized silver (Ag) dendrites. The 4-MPBA functionalized Ag dendritic SERS substrate was used to capture the bacterial cells and enhance the bacterial signal. Salmonella, a significantly important food pathogen, was used as the representative strain to optimize and evaluate the developed method. The capture efficiency for Salmonella enterica subsp enterica BAA1045 (SE1045) was 84...
January 14, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28088421/the-influence-of-ultra-pasteurization-by-indirect-heating-versus-direct-steam-injection-on-skim-and-2-fat-milks
#10
A P Lee, D M Barbano, M A Drake
Fluid milk is traditionally pasteurized by high temperature, short time (HTST) pasteurization, which requires heating to at least 72°C for 15 s. Ultra-pasteurization (UP) extends milk shelf life and is defined as heating to at least 138°C for 2 s. The UP process can be done by indirect heating (IND) or by direct steam injection (DSI). The influence of these 2 UP methods on milk flavor has not been widely investigated. The objective of this study was to compare the effect of HTST, IND-UP, and DSI-UP on sensory perception of fluid milk...
March 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28088417/milk-fat-threshold-determination-and-the-effect-of-milk-fat-content-on-consumer-preference-for-fluid-milk
#11
K S McCarthy, K Lopetcharat, M A Drake
Milk consumption in the United States has been in decline since the 1960s. Milk fat plays a critical role in sensory properties of fluid milk. The first objective of this study was to determine the change in percent milk fat needed to produce a detectable or just noticeable difference (JND) to consumers in skim, 1%, 2%, and whole milks. The second objective was to evaluate how milk fat affected consumer preferences for fluid milk. Threshold tests were conducted to determine the JND for each reference milk (skim, 1%, 2%, and whole milk), with a minimum of 60 consumers for each JND...
March 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28088406/short-communication-effects-of-vacuum-freeze-drying-on-inactivation-of-cronobacter-sakazakii-atcc29544-in-liquid-media-with-different-initial-inoculum-levels
#12
Rui Jiao, Jina Gao, Xiyan Zhang, Maofeng Zhang, Jiren Chen, Qingping Wu, Jumei Zhang, Yingwang Ye
Vacuum freeze-drying is an important food-processing technology for valid retention of nutrients and bioactive compounds. Cronobacter sakazakii has been reported to be associated with severe infections in neonates through consumption of contaminated powdered infant formula. In this study, effects of vacuum freeze-drying treatment for 12, 24, and 36 h on inactivation of C. sakazakii with different initial inoculum levels in sterile water, tryptic soy broth (TSB), skim milk, and whole milk were determined. Results indicated that the lethality rate of C...
March 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28088252/quantification-of-whey-protein-content-in-infant-formulas-by-sds-cge
#13
Ping Feng, Christophe Fuerer, Adrienne McMahon
Protein separation by SDS-CGE (Sodium Dodecyl Sulfate - Capillary Gel Electrophoresis) followed by UV absorption at 220nm allows quantification of major proteins in raw milk. In processed dairy samples such as skim milk powder and infant formulas, signals from individual proteins are less resolved, but caseins still migrate as one family between two groups of whey proteins. In the first group, α-Lactalbumin and β-Lactoglobulin migrate as two distinct peaks. Lactosylated adducts show delayed migration times and interfere with peak separation, but both native and modified forms, as well as other low molecular weight whey proteins, still elute before the caseins...
November 18, 2016: Journal of AOAC International
https://www.readbyqxmd.com/read/28081252/corn-soy-blend-fortified-with-phosphorus-to-prevent-refeeding-hypophosphatemia-in-undernourished-piglets
#14
Anne-Louise Hother, Mikkel Lykke, Torben Martinussen, Hanne Damgaard Poulsen, Christian Mølgaard, Per Torp Sangild, André Briend, Christian Fink Hansen, Henrik Friis, Kim F Michaelsen, Thomas Thymann
BACKGROUND: Phosphorus (P) levels in refeeding diets are very important as undernourished children are at risk of hypophosphatemia during refeeding. For this reason, conventional corn-soy-blends (CSB) have been reformulated by the World Food Programme to obtain a mono-calcium-phosphate fortified product (CSB+) and a product further fortified with skim milk powder (CBS++). METHODS: Using a piglet model of undernourished children, we hypothesized that feeding of CSB+, CSB++ or CSB+ with added whey permeate (CSB+/wp) would help to prevent refeeding hypophosphatemia...
2017: PloS One
https://www.readbyqxmd.com/read/28064480/impact-of-the-skim-milk-powder-manufacturing-process-on-the-flavor-of-model-white-chocolate
#15
Ashleigh Stewart, Alistair S Grandison, Angela Ryan, Daniel Festring, Lisa Methven, Jane K Parker
Milk powder is an important ingredient in the confectionery industry, but its variable nature has consequences for the quality of the final confectionary product. This paper demonstrates that skim milk powders (SMP) produced using different (but typical) manufacturing processes, when used as ingredients in the manufacture of model white chocolates, had a significant impact on the sensory and volatile profiles of the chocolate. SMP was produced from raw bovine milk using either low or high heat treatment, and a model white chocolate was prepared from each SMP...
February 7, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28063330/endolysin-lyssa97-is-synergistic-with-carvacrol-in-controlling-staphylococcus-aureus-in-foods
#16
Yoonjee Chang, Hyunjin Yoon, Dong-Hyun Kang, Pahn-Shick Chang, Sangryeol Ryu
LysSA97 is an endolysin encoded by the bacteriophage SA97, the genome sequence of which has been recently revealed. LysSA97 has lytic activity against a variety of Staphylococcus strains that cause foodborne illness. In order to improve its potential as a biocontrol agent against Staphylococcus, various types of essential oil-derived active compounds were tested in combination with LysSA97; carvacrol exhibited significant synergistic effects when combined with LysSA97. The synergistic antimicrobial activity between endolysin and carvacrol in food products, including milk and beef, were investigated...
March 6, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28052193/distribution-of-animal-drugs-among-curd-whey-and-milk-protein-fractions-in-spiked-skim-milk-and-whey
#17
Nancy W Shappell, Weilin L Shelver, Sara J Lupton, Wendy Fanaselle, Jane M Van Doren, Heldur Hakk
It is important to understand the partitioning of drugs in processed milk and milk products, when drugs are present in raw milk, in order to estimate the potential consumer exposure. Radioisotopically labeled erythromycin, ivermectin, ketoprofen, oxytetracycline, penicillin G, sulfadimethoxine, and thiabendazole were used to evaluate the distribution of animal drugs among rennet curd, whey, and protein fractions from skim cow milk. Our previous work reported the distribution of these same drugs between skim and fat fractions of milk...
February 1, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28042050/novel-technical-strategies-to-optimize-cryopreservation-of-goat-semen-using-cholesterol-loaded-cyclodextrin
#18
Vianney M Salmon, Pierre Leclerc, Janice L Bailey
Artificial insemination is well-established in dairy cattle, with sires housed in commercial studs for processing. In some species, however, sires located on-farm are used for artificial insemination by shipping their semen to an off-site laboratory for processing within 24 h of collection. To expedite semen transport from the farm to laboratory, protocols must be uncomplicated. For goat semen, an obstacle is the seminal plasma, which must be removed because it contains proteins that impede sperm quality. Our objective is to develop a simple strategy to transiently store goat semen for 24 h prior to freezing...
December 29, 2016: Cryobiology
https://www.readbyqxmd.com/read/28035151/effect-of-concentration-homogenization-and-stabilizing-salts-on-heat-stability-and-rheological-properties-of-cow-skim-milk-ultrafiltered-retentate
#19
Ganga Sahay Meena, Ashish Kumar Singh, Sanket Borad, Narender Raju Panjagari
Ultrafiltration (UF) of skimmed milk altered the composition of UF retentate and decreased the heat stability. Heat stability further reduced upon its subsequent homogenization or diafiltration. Poor heat stability of UF retentate restricts its processing at elevated temperatures. Therefore, this study was aimed to investigate the effect of protein concentration, homogenization and addition of stabilizing salts on the heat stability and rheological properties of UF retentates. Changes in the heat stability of fivefold homogenized UF retentate (5× HUFR) was studied in the pH range of 6...
November 2016: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28017451/cholesterol-loaded-cyclodextrin-improves-ram-sperm-cryoresistance-in-skim-milk-extender
#20
RANDOMIZED CONTROLLED TRIAL
Vianney M Salmon, François Castonguay, Vincent Demers-Caron, Pierre Leclerc, Janice L Bailey
Cholesterol-loaded cyclodextrin (CLC) is known to improve ram sperm cryosurvival. This study expands on previous research to: (1) determine the mechanism by which CLC improves ram sperm cryosurvival and (2) compare the efficiency of a novel, skim milk-based extender containing CLC to a traditional egg yolk-based extender. Hypothesis #1 was that CLC enhances membrane cholesterol content to increase the resistance of ram sperm to cold and osmotic stress, thereby improving cryosurvival. We first assessed the ability of fresh sperm treated with CLC to withstand cold shock...
February 2017: Animal Reproduction Science
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