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https://www.readbyqxmd.com/read/29922266/pantothenic-acid-vitamin-c-and-biotin-play-important-roles-in-the-growth-of-lactobacillus-helveticus
#1
Chunxiao Yao, Jiandong Chou, Tao Wang, Hongfei Zhao, Bolin Zhang
Lactobacillus helveticus is an important lactic acid bacterium. The strains used in this study have proven probiotic function, and the potential to produce functional dairy products and bioactive peptides. To explore the effects of vitamins on the growth of L. helveticus , a chemically defined medium was designed and nine vitamins were tested. Pantothenic acid (Vb5 ), vitamin C (Vc), and biotin were necessary for the growth of L. helveticus CICC 22171. These three vitamins had an important effect on the glucose metabolism and energy metabolism of strain CICC 22171...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29904375/quantifying-the-responses-of-three-bacillus-cereus-strains-in-isothermal-conditions-and-during-spray-drying-of-different-carrier-agents
#2
Verônica O Alvarenga, Fernanda B Campagnollo, Arthur K R Pia, Deborah A Conceição, Yuri Abud, Celso Sant'Anna, Miriam D Hubinger, Anderson S Sant'Ana
Spray drying is a widely used method for producing milk powder. This process is not aimed to cause microbial inactivation, thus sporeforming bacteria may be abundant in the microbiota of milk powder. The first aim of this study was to determine the inactivation kinetics parameters in capillary tubes of three Bacillus cereus strains (436, B63, 540) in three menstrua (whole milk, phosphate buffer, and talc suspension) at 90, 100, and 110°C. D -values for B. cereus in the three menstrua were not significantly different at the highest tested temperature ( p > 0...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29885892/technical-note-development-and-validation-of-an-hplc-method-for-the-quantification-of-tocopherols-in-different-types-of-commercial-cow-milk
#3
G Niero, M Penasa, J Berard, M Kreuzer, M Cassandro, M De Marchi
In the present study, a methanol-fluorescence-based HPLC method was validated for its use to quantify α-tocopherol and γ-tocopherol in raw milk, whole UHT milk, partially skimmed UHT milk, whole pasteurized milk, and partially skimmed pasteurized milk. Repeatability and reproducibility, calculated as relative standard deviation of 10 measurements within the same day and 30 measurements across 3 d, respectively, were always below 5% for both tocopherols concentrations and retention times. Recovery was assessed through 3 spiking levels and it ranged from 89 to 107%...
June 6, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29861337/implementation-of-the-use-of-skimmed-breast-milk-and-the-registered-dietitian-nutritionist-s-role
#4
Brittani Clark, Melissa Froh, Elizabeth Polzin
No abstract text is available yet for this article.
May 31, 2018: Journal of the Academy of Nutrition and Dietetics
https://www.readbyqxmd.com/read/29805277/buffering-capacity-of-dairy-powders-and-their-effect-on-yoghurt-quality
#5
Miji Kim, Sejong Oh, Jee-Young Imm
Preheating conditions (low-, medium-, and high heat-) did not significantly affect the buffering capacity (BC) of skim milk powder (SMP), whereas the level of demineralization significantly affected the BC of whey powders (WP). Heat treatment (85°C for 30 min) of both SMP and WP (90% demineralized) mixtures (88:12, 76:24, 64:36 and 52:48; SMP:WP) resulted in a reduced BC, and the extent of this reduction increased with the proportion of WP increased in the samples. High-buffering milk prepared by the addition of phosphate salts (40 mM NaH2 PO4 and 60 mM Na2 HPO4 ) delayed the rate of pH decline during yoghurt fermentation...
April 2018: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/29803416/effects-of-milk-heat-treatment-and-solvent-composition-on-physicochemical-and-selected-functional-characteristics-of-milk-protein-concentrate
#6
Yingchen Lin, Alan L Kelly, James A O'Mahony, Timothy P Guinee
Milk protein concentrate (MPC) powders (~81% protein) were made from skim milk that was heat treated at 72°C for 15 s (LHMPC) or 85°C for 30 s (MHMPC). The MPC powder was manufactured by ultrafiltration and diafiltration of skim milk at 50°C followed by spray drying. The MPC dispersions (4.02% true protein) were prepared by reconstituting the LHMPC and MHMPC powders in distilled water (LHMPCw and MHMPCw , respectively) or milk permeate (LHMPCp and MHMPCp , respectively). Increasing milk heat treatment increased the level of whey protein denaturation (from ~5 to 47% of total whey protein) and reduced the concentrations of serum protein, serum calcium, and ionic calcium...
May 23, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29803413/psychrotolerant-spore-former-growth-characterization-for-the-development-of-a-dairy-spoilage-predictive-model
#7
A J Buehler, N H Martin, K J Boor, M Wiedmann
Psychrotolerant spore-forming bacteria represent a major challenge regarding microbial spoilage of fluid milk. These organisms can survive most conventional pasteurization regimens and subsequently germinate and grow to spoilage levels during refrigerated storage. To improve predictions of fluid milk shelf life and assess different approaches to control psychrotolerant spore-forming bacteria in the fluid milk production and processing continuum, we developed a predictive model of spoilage of fluid milk due to germination and growth of psychrotolerant spore-forming bacteria...
May 23, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29803220/manufacturing-of-curd-products-of-increased-biological-value-for-the-elderly-from-dried-components
#8
Ludmila A Zabodalova, Maria S Belozerova, Tatiana N Evstigneeva
BACKGROUND: In recent years, the number of elderly people has increased, and the diseases that arise in old age are associated, amongst other factors, with malnutrition. In the elderly, the need for primary nutrients and energy changes, so the development of food products intended for this particular group of people is becom- ing increasingly important. The purpose of this research is to work out the composition of and technology for producing low-fat curd products from raw milk and vegetable components...
April 2018: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/29792962/effect-of-casein-and-inulin-addition-on-physico-chemical-characteristics-of-low-fat-camel-dairy-cream
#9
Leila Ziaeifar, Mohsen Labbafi Mazrae Shahi, Maryam Salami, Gholam R Askari
The effect of the addition of the camel casein fraction on some physico-chemical properties of low fat camel milk cream was studied. Oil-in-water emulsions, 25, 30, and 35 (w/w) fat, were prepared using inulin, camel skim milk, milk fat and variable percentages of casein (1, 2, and 3% w/w). The droplet size, ζ-potential, surface protein concentration, viscosity and surface tension of low fat dairy creams was measured. Cream containing 2% (w/w) casein had better stability. The modifications in physico-chemical properties appeared to be driven by changes in particle size distribution caused by droplet aggregation...
May 21, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29786853/cysteamine-modified-gold-nanoparticles-as-a-colorimetric-sensor-for-the-rapid-detection-of-gentamicin
#10
Joshua C Gukowsky, Chen Tan, Zexiang Han, Lili He
A simple, rapid, and specific colorimetric method for gentamicin detection using cysteamine-modified gold nanoparticles (cys-AuNPs) has been developed. The maximum residue limits of gentamicin allowed in foods are typically below 100 nM, so an effective detection method for low concentrations of the drug is required. The aggregation of gold nanoparticles (AuNPs) was used as the basis for this method, and adding cysteamine to the AuNPs helped to enhance their aggregative abilities. The cys-AuNPs are capable of detecting gentamicin concentrations as low as 12...
May 22, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29784297/destabilization-of-uht-milk-by-protease-aprx-from-pseudomonas-fluorescens-and-plasmin
#11
Chunyue Zhang, Etske Bijl, Kasper Hettinga
Destabilization of UHT milk during its shelf life is mainly promoted by the residual proteolytic activity attributed to the psychrotrophic bacterial proteases and native milk proteases. In this study, we built skim UHT milk-based model systems to which either the major bacterial protease (AprX from Pseudomonas fluorescens), or the major native milk protease (plasmin) was added, to allow a direct comparison between the destabilization of skim UHT milk by both categories of enzymes. The physical and chemical properties were studied during 6 weeks...
October 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29778653/survival-and-phosphate-solubilisation-activity-of-desiccated-formulations-of-penicillium-bilaiae-and-aspergillus-niger-influenced-by-water-activity
#12
Nelly Sophie Raymond, Dorette Müller Stöver, Lars Stoumann Jensen, Sebastian Håkansson
The impact of formulation and desiccation on the shelf life of phosphate (P)-solubilising microorganisms is often under-studied, particularly relating to their ability to recover P-solubilisation activity. Here, Penicilllium bilaiae and Aspergillus niger were formulated on vermiculite (V) alone, or with the addition of protectants (skimmed milk (V + SM) and trehalose (V + T)), and on sewage sludge ash with (A + N) and without nutrients (A), and dried in a convective air dryer. After drying, the spore viability of P...
May 17, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29778512/dairy-intake-and-acne-development-a-meta-analysis-of-observational-studies
#13
Mohadeseh Aghasi, Mahdieh Golzarand, Sakineh Shab-Bidar, Azadeh Aminianfar, Mahsa Omidian, Fatemeh Taheri
BACKGROUND & AIMS: In the past, some observational studies have been carried out on the relationship between milk and dairy intake and risk of acne occurrence; however, their results were conflicting. This study is a meta-analysis and dose-response analysis designed to evaluate the relationship between milk and dairy products and acne development. MATERIALS & METHODS: Data of the study were searched and collected from Pubmed/Medline, Scopus, Web of Science, and Embase databases...
May 8, 2018: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/29772827/physicochemical-nutritional-and-organoleptic-characterization-of-a-skimmed-goat-milk-fermented-with-the-probiotic-strain-lactobacillus-plantarum-c4
#14
Miriam Moreno-Montoro, Miguel Navarro-Alarcón, Triana Bergillos-Meca, Rafael Giménez-Martínez, Silvia Sánchez-Hernández, Manuel Olalla-Herrera
The benefits of goat milk, fermented milks, and probiotics for the humans are well documented. In this study, a novel fermented goat milk was manufactured with the putative probiotic strain Lactobacillus plantarum C4 together with L. bulgaricus and Streptococcus thermophilus . Ultrafiltration was chosen as the skimmed milk concentration method because it produced the best viscosity and syneresis and a high casein content. The viability rate of all bacterial strains was >10⁷ cfu/mL, even after 5 weeks of storage or after in vitro gastrointestinal digestion, which is especially important for exertion of the probiotic strain functionalities...
May 17, 2018: Nutrients
https://www.readbyqxmd.com/read/29760329/delivery-of-curcumin-using-skim-milk-or-oil-in-water-emulsions-effect-of-the-matrices-on-cellular-uptake
#15
Anilda Guri, İbrahim Gülseren, Elena Arranz, Milena Corredig
To enhance the curcumin delivery in a variety of food grade matrices namely spray dried ethanolic curcumin in fresh skim milk (Spray dried Cu-SM), a fresh mixture of ethanolic curcumin and skim milk (Fresh Cu-SM) a powder mixture of curcumin and skim milk powder (Powder Cu-SMP) and oil in water emulsion (Emulsion) were studied. The cellular uptake of curcumin from the respective matrices was studied on Caco-2 cell monolayers. Spray dried Cu-SM showed higher encapsulation efficiency compared to a corresponding Powder Cu-SMP and an oil-in-water emulsion (40% oil) bearing curcumin...
May 15, 2018: Journal of Oleo Science
https://www.readbyqxmd.com/read/29756971/the-relationship-between-socioeconomic-status-and-beverage-consumption-in-children-the-cuenca-study
#16
Marta Milla Tobarra, Antonio García Hermoso, Noelia Lahoz García, Blanca Notario Pacheco, Lidia Lucas de la Cruz, Diana P Pozuelo Carrascosa, María José García Meseguer, Vicente A Martínez Vizcaíno
INTRODUCTION: beverage consumption constitutes a source of children's daily energy intake. Some authors have suggested that consumption of caloric beverages is higher in children with a low socioeconomic position because families limit their spending on healthy food in order to save money. OBJECTIVE: the aim of this study was to explore the relationship between socioeconomic status and Spanish children's beverage consumption. METHODS: a cross-sectional study was conducted in a sub-sample of 182 children (74 girls) aged 9-11 from the province of Cuenca (Spain)...
January 19, 2018: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/29753488/gastric-digestion-of-milk-protein-ingredients-study-using-an-in-vitro-dynamic-model
#17
Xin Wang, Aiqian Ye, Quanquan Lin, Jianzhong Han, Harjinder Singh
The coagulation behavior and the kinetics of protein hydrolysis of skim milk powder, milk protein concentrate (MPC), calcium-depleted MPC, sodium caseinate, whey protein isolate (WPI), and heated (90°C, 20 min) WPI under gastric conditions were examined using an advanced dynamic digestion model (i.e., a human gastric simulator). During gastric digestion, these protein ingredients exhibited various pH profiles as a function of the digestion time. Skim milk powder and MPC, which contained casein micelles, formed cohesive, ball-like curds with a dense structure after 10 min of digestion; these curds did not disintegrate over 220 min of digestion...
May 9, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29748614/nanovesicle-mediated-delivery-of-anticancer-agents-effectively-induced-cell-death-and-regressed-intrahepatic-tumors-in-athymic-mice
#18
Joseph George, Irene K Yan, Tushar Patel
Hepatocellular carcinoma is highly resistant to chemotherapy. Here we evaluated the use and efficacy of milk-derived nanovesicles (MNV) as an approach to improve delivery of anticancer agents into HCC cells and intrahepatic tumors. We developed a protocol for isolation of MNVs from skim milk using ultracentrifugation, and characterized using nanoparticle tracking analysis (NTA) and electron microscopy. MNVs were loaded with doxorubicin (dox-MNV) or miR221 antisense oligonucleotides (anti-miR221-MNV), and further evaluated using spectrophotometry, NTA, and zeta potential measurements...
May 10, 2018: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29737161/anti-cancer-and-anti-migration-effects-of-combinatorial-treatment-with-5-fluorouracil-and-lactobacillus-paracasei-subsp-paracasei-ntu-101-fermented-skim-milk-extracts-on-colorectal-cancer-cells
#19
Chia-Yuan Chang, Tzu-Ming Pan
Colorectal cancer (CRC) is one of the most prevalent cancers worldwide. Metabolites of lactic acid bacteria (LAB) have anti-cancer and anti-metastasis capacity. This study aimed to investigate the chemotherapeutic combination effect of Lactobacillus paracasei subsp. paracasei NTU 101-fermented skim milk (NTU101-FM) extracts and the chemotherapeutic drug 5-fluorouracil (5-FU) in a cellular CRC model. NTU101-FM extracts effectively reduced CRC cell viability but was not cytotoxic to colon epithelial cells. Moreover, they increased RAW 264...
May 8, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29730482/development-and-optimization-of-new-culture-media-for-acanthamoeba-spp-protozoa-amoebozoa
#20
Tania Martín-Pérez, Angel Criado-Fornelio, Manuel Ávila-Blanco, Jorge Pérez-Serrano
The isolation and growth in axenic liquid media of Acanthamoeba strains is necessary in order to carry out primary in vitro drug screening. Amoebic isolates which are hard to grow in the current liquid media have been reported. Such circumstances hampers the ability of conducting drug sensitivity tests. Therefore, finding suitable universal growth media for Acanthamoeba species is required. The present study was aimed at the development of liquid medium suitable for growing a fastidious (F) genotype T3 Acanthamoeba isolate, and eventually for other genotypes of this genus as well...
April 12, 2018: European Journal of Protistology
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