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https://www.readbyqxmd.com/read/28333357/dietary-patterns-foods-and-nutrients-in-male-fertility-parameters-and-fecundability-a-systematic-review-of-observational-studies
#1
Albert Salas-Huetos, Mònica Bulló, Jordi Salas-Salvadó
BACKGROUND: Infertility is a global public health issue, affecting 15% of all couples of reproductive age. Male factors, including decreased semen quality, are responsible for ~25% of these cases. The dietary pattern, the components of the diet and nutrients have been studied as possible determinants of sperm function and/or fertility. OBJECTIVE AND RATIONALE: Previous systematic reviews have been made of the few heterogeneous low-quality randomized clinical trials (RCTs) conducted in small samples of participants and investigating the effect of specific nutrients and nutritional supplements on male infertility...
March 10, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/28333255/alkaline-protease-contributes-to-pyocyanin-production-in-pseudomonas-aeruginosa
#2
Kazuhiro Iiyama, Eigo Takahashi, Jae Man Lee, Hiroaki Mon, Mai Morishita, Takahiro Kusakabe, Chisa Yasunaga-Aoki
The role of the alkaline protease (AprA) in pyocyanin production in Pseudomonas aeruginosa was investigated. AprA was overproduced when a plasmid carrying the aprA gene was introduced to an aprA-deletion mutant strain, EG03; thus, aprA-complemented EG03 was used as an overproducing strain. The complemented strain produced higher pyocyanin than the mutant strain in all commercially available media evaluated. Particularly, pyocyanin production was higher in the complemented than in the parental strain in brain-heart infusion and tryptic soy broths...
March 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28321408/novel-sequential-screening-and-enhanced-production-of-fibrinolytic-enzyme-by-bacillus-sp-ind12-using-response-surface-methodology-in-solid-state-fermentation
#3
Ponnuswamy Vijayaraghavan, P Rajendran, Samuel Gnana Prakash Vincent, Arumugaperumal Arun, Naif Abdullah Al-Dhabi, Mariadhas Valan Arasu, Oh Young Kwon, Young Ock Kim
Fibrinolytic enzymes have wide applications in clinical and waste treatment. Bacterial isolates were screened for fibrinolytic enzyme producing ability by skimmed milk agar plate using bromocresol green dye, fibrin plate method, zymography analysis, and goat blood clot lysis. After these sequential screenings, Bacillus sp. IND12 was selected for fibrinolytic enzyme production. Bacillus sp. IND12 effectively used cow dung for its growth and enzyme production (687 ± 6.5 U/g substrate). Further, the optimum bioprocess parameters were found out for maximum fibrinolytic enzyme production using cow dung as a low cost substrate under solid-state fermentation...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28318591/reduction-of-mycobacterium-avium-ssp-paratuberculosis-in-colostrum-development-and-validation-of-2-methods-one-based-on-curdling-and-one-based-on-centrifugation
#4
M Verhegghe, G Rasschaert, L Herman, K Goossens, L Vandaele, K De Bleecker, G Vlaemynck, M Heyndrickx, J De Block
The aim of this study was to develop and validate 2 protocols (for use on-farm and at a central location) for the reduction of Mycobacterium avium ssp. paratuberculosis (MAP) in colostrum while preserving beneficial immunoglobulins (IgG). The on-farm protocol was based on curdling of the colostrum, where the IgG remain in the whey and the MAP bacteria are trapped in the curd. First, the colostrum was diluted with water (2 volumes colostrum to 1 volume water) and 2% rennet was added. After incubation (1 h at 32°C), the curd was cut and incubated again, after which whey and curd were separated using a cheesecloth...
March 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28318581/characterization-of-the-chemical-structures-and-physical-properties-of-exopolysaccharides-produced-by-various-streptococcus-thermophilus-strains
#5
U Pachekrepapol, J A Lucey, Y Gong, R Naran, P Azadi
Exopolysaccharides (EPS) produced by some lactic acid bacteria are often used by the dairy industry to improve the rheological and physical properties of yogurt, but the relationship between their structure and functional effect is still unclear. The EPS from different species, or different strains from the same species, may differ in terms of molar mass, repeating unit structure, and EPS yield during fermentation of milk. This study aimed to characterize the detailed properties of EPS produced from 7 strains of Streptococcus thermophilus, which is one of the key cultures used for yogurt manufacture...
March 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28316475/optimization-of-manufacturing-conditions-for-improving-storage-stability-of-coffee-supplemented-milk-beverage-using-response-surface-methodology
#6
Sung-Il Ahn, Jun-Hong Park, Jae-Hoon Kim, Duk-Geun Oh, Moojoong Kim, Donghwa Chung, Jin-Woo Jhoo, Gur-Yoo Kim
This study aimed at optimizing the manufacturing conditions of a milk beverage supplemented with coffee, and monitoring its physicochemical and sensory properties during storage. Raw milk, skim milk powder, coffee extract, and emulsifiers were used to manufacture the beverage. Two sucrose fatty acid esters, F110 and F160, were identified as suitable emulsifiers. The optimum conditions for the beverage manufacture, which can satisfy two conditions at the same time, determined by response surface methodology (RSM), were 5,000 rpm primary homogenization speed and 0...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/28315420/eating-behaviour-is-associated-with-eating-frequency-and-food-consumption-in-6-8-year-old-children-the-physical-activity-and-nutrition-in-children-panic-study
#7
H Jalkanen, V Lindi, U Schwab, S Kiiskinen, T Venäläinen, L Karhunen, T A Lakka, A M Eloranta
The association between eating behaviour and dietary factors has been studied narrowly in children. Therefore, we investigated whether eating frequency and food consumption are influenced by eating behaviour in a population sample of 406 children aged 6-8 years. We assessed features of eating behaviour by the Children's Eating Behaviour Questionnaire and dietary factors by a 4-day food record. The results showed that enjoyment of food was directly associated with a number of main meals (p = 0.041) and consumption of vegetables (p = 0...
March 15, 2017: Appetite
https://www.readbyqxmd.com/read/28298694/isolation-molecular-characterization-and-screening-of-indigenous-lactobacilli-for-their-abilities-to-produce-bioactive-conjugated-linoleic-acid-cla
#8
Dinesh Kumar Dahiya, Anil Kumar Puniya
Ingestion of conjugated linoleic acid poised many health benefits; however, amount of CLA one can get through generalized diet in is inadequate in exerting the desired benefits. Therefore, presence of CLA producing lactobacilli in dairy fermented foods has a tremendous potential to increase the CLA content. Therefore, present study was focused to isolate and characterize CLA producing lactobacilli from different dairy products and human faeces. Arguably, 283 lactobacilli were isolated from various sources and tested for CLA production...
March 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28294173/the-effect-of-uht-processed-dairy-milk-on-cardio-metabolic-risk-factors
#9
C K Hansen, L Klingenberg, L B Larsen, J K Lorenzen, K V Sørensen, A Astrup
BACKGROUND/OBJECTIVES: Cardiovascular disease (CVD) remains a major cause of death worldwide. Whereas dairy generally is associated with a neutral or a beneficial CVD effect, the consumption of ultra-high temperature (UHT)-treated milk has been reported to increase levels of low-density lipoprotein cholesterol (LDL-C) in an uncontrolled study. Our aim was to examine whether semi-skimmed UHT dairy milk increases the risk of CVD development compared with pasteurized (PAST) dairy milk in overweight healthy adults...
March 15, 2017: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28284696/utilizing-whey-protein-isolate-and-polysaccharide-complexes-to-stabilize-aerated-dairy-gels
#10
Emily O'Chiu, Bongkosh Vardhanabhuti
Heated soluble complexes of whey protein isolate (WPI) with polysaccharides may be used to modify the properties of aerated dairy gels, which could be formulated into novel-textured high-protein desserts. The objective of this study was to determine the effect of polysaccharide charge density and concentration within a WPI-polysaccharide complex on the physical properties of aerated gels. Three polysaccharides having different degrees of charge density were chosen: low-methoxyl pectin, high-methoxyl type D pectin, and guar gum...
March 8, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28282925/associations-between-maternal-body-composition-and-appetite-hormones-and-macronutrients-in-human-milk
#11
Sambavi Kugananthan, Zoya Gridneva, Ching T Lai, Anna R Hepworth, Peter J Mark, Foteini Kakulas, Donna T Geddes
Human milk (HM) appetite hormones and macronutrients may mediate satiety in breastfed infants. This study investigated associations between maternal adiposity and concentrations of HM leptin, adiponectin, protein and lactose, and whether these concentrations and the relationship between body mass index and percentage fat mass (%FM) in a breastfeeding population change over the first year of lactation. Lactating women (n = 59) provided milk samples (n = 283) at the 2nd, 5th, 9th and/or 12th month of lactation...
March 9, 2017: Nutrients
https://www.readbyqxmd.com/read/28276067/in-vitro-and-in-vivo-evaluation-of-weissella-cibaria-and-lactobacillus-plantarum-for-their-protective-effect-against-cadmium-and-lead-toxicities
#12
Opeyemi Ojekunle, Kolawole Banwo, Abiodun I Sanni
Thirty-two lactic acid bacteria (LAB) isolates were obtained from fermenting cassava mash and wara (African soft cheese) and screened for their resistance to cadmium and lead toxicities at 550 to 1050 mg l(-1) and probiotic potentials. Four LAB strains that tolerated the heavy metals at 1050 mg l(-1) were selected for antioxidative capacities, tolerance to acid, bile salts and simulated gastric and intestinal tract and safety status. The results revealed that Weissella cibaria WD2 and Lactobacillus plantarum CaD1 exhibited comparatively higher antioxidative capacities, survived in simulated gastric and intestinal transit, tolerated acid and bile salt and possessed safety status...
March 9, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28252360/optimization-of-ph-temperature-and-cacl2-concentrations-for-ricotta-cheese-production-from-buffalo-cheese-whey-using-response-surface-methodology
#13
Abdul Ahid Rashid, Nuzhat Huma, Tahir Zahoor, Muhammad Asgher
The recovery of milk constituents from cheese whey is affected by various processing conditions followed during production of Ricotta cheese. The objective of the present investigation was to optimize the temperature (60-90 °C), pH (3-7) and CaCl2 concentration (2·0-6·0 mm) for maximum yield/recovery of milk constituents. The research work was carried out in two phases. In 1st phase, the influence of these processing conditions was evaluated through 20 experiments formulated by central composite design (CCD) keeping the yield as response factor...
February 2017: Journal of Dairy Research
https://www.readbyqxmd.com/read/28249701/effect-of-season-on-scrotal-circumference-semen-characteristics-seminal-plasma-composition-and-spermatozoa-motility-during-liquid-storage-in-inra180-rams
#14
Anass Benmoula, Abdelmoughit Badi, Moussa El Fadili, Kaoutar El Khalil, Larbi Allai, Abderaouf El Hilali, Bouchra El Amiri
The present study was undertaken to assess the effect of seasons on scrotal circumference, semen characteristics, seminal plasma composition, and sperm motility during liquid storage of INRA180 rams. The semen was collected from five mature INRA180 rams (2-3 years of age) during one year (from April 2014 to March 2015). Scrotal circumferences, semen characteristics, some biochemical parameters of seminal plasma were evaluated. Immediately after collection and evaluation, the semen was pooled and extended in skim milk (SM) at 15°C to reach 0...
February 22, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28238445/pentoxifylline-effects-on-capacitation-and-fertility-of-stallion-epididymal-sperm
#15
P N Guasti, G A Monteiro, R R D Maziero, M T Carmo, J A Dell'Aqua, A M Crespilho, E A Rifai, F O Papa
The aims of this study were to determinate whether pentoxifylline (PTX) increases the motion parameters of fresh and frozen-thawed equine epididymal spermatozoa, to evaluate the tyrosine phosphorylation of frozen-thawed epididymal sperm in the presence of PTX and to determine whether the PTX-treatment of stallion epididymal sperm prior to freezing improves the fertility response of mares to a reduced number of spermatozoa per insemination dose. Fifty epididymis were flushed with a skim milk based extender with or without PTX...
April 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28222216/biochemical-characterization-of-an-extracellular-heat-stable-protease-from-serratia-liquefaciens-isolated-from-raw-milk
#16
François Baglinière, Rafael Locatelli Salgado, Cleonice Aparecida Salgado, Maria Cristina Dantas Vanetti
The protease Ser2 secreted by the psychrotrophic strain Serratia liquefaciens L53, a highly proteolytic strain isolated from Brazilian raw milk was purified and characterized. Using azocasein as substrate, Ser2 exhibited activity in a wide range of pH (5 to 10) and temperature (4 to 60 °C). The optimal activity was detected at pH 8.0 and at a temperature of 37 °C. This protease, still active at 4, 7, and 10 °C, was strongly inhibited by chelating agents and by dithiothreitol, a reducing agent. These results confirmed that Ser2 belongs to the peptidase family M10 and requires Ca(2+) , Zn(2+) , and disulfide bridges for stability...
February 21, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28221936/evaluation-of-a-recirculating-dipper-well-combined-with-ozone-sanitizer-for-control-of-foodborne-pathogens-in-food-service-operations
#17
Giselle Almeida, Kristen E Gibson
In the retail food service industry, small countertop sinks, or dipper wells, are utilized to rinse and store serving utensils between uses. These dipper wells are designed to operate under a constant flow of water, which serves both to prevent the accumulation of microorganisms and to aid in the cleanliness of the dipper well itself. Here, a recirculating dipper well ozone sanitation system (DWOSS) was evaluated for the control and inactivation of Escherichia coli , Listeria innocua , PRD1 bacteriophage, and Staphylococcus aureus present on a stainless steel disher...
September 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28211112/impact-of-a-targeted-direct-marketing-price-promotion-intervention-buywell-on-food-purchasing-behaviour-by-low-income-consumers-a-randomised-controlled-trial
#18
M Stead, A M MacKintosh, A Findlay, L Sparks, A S Anderson, K Barton, D Eadie
BACKGROUND: Price promotions are a promising intervention for encouraging healthier food purchasing. We aimed to assess the impact of a targeted direct marketing price promotion combined with healthy eating advice and recipe suggestions on the purchase of selected healthier foods by low income consumers. METHODS: We conducted a randomised controlled trial (n = 53 367) of a direct marketing price promotion (Buywell) combined with healthy eating advice and recipe suggestions for low income consumers identified as 'less healthy' shoppers...
February 17, 2017: Journal of Human Nutrition and Dietetics: the Official Journal of the British Dietetic Association
https://www.readbyqxmd.com/read/28208254/electrochemical-detection-of-escherichia-coli-from-aqueous-samples-using-engineered-phages
#19
Danhui Wang, Juhong Chen, Sam R Nugen
In this study, an enzyme-based electrochemical method was developed for the detection of Escherichia coli (E. coli) using the T7 bacteriophages engineered with lacZ operon encoding for beta-galactosidase (β-gal). The T7lacZ phages can infect E. coli, and have the ability to trigger the overexpression of β-gal during the infection of E. coli. The use of the engineered phages resulted in a more sensitive detection of E. coli by (1) overexpression of β-gal in E. coli during the specific infection and (2) release of the endogenous intracellular β-gal from E...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28202335/purification-and-characterization-of-a-milk-clotting-aspartic-protease-from-withania-coagulans-fruit
#20
Mahmoud Salehi, Mahmoud Reza Aghamaali, Reza H Sajedi, S Mohsen Asghari, Eisa Jorjani
Withania coagulans fruit has traditionally been used as milk coagulant. The present study reports the purification and characterization of an aspartic protease from W. coagulans fruit. The enzyme was purified via fractional ammonium sulfate precipitation and cation exchange chromatography. SDS-PAGE analysis revealed the presence of a monomeric protein with molecular weight of 31kDa. Proteolytic activity (PA) of the protease was evaluated using casein, and the milk-clotting activity (MCA) was analyzed by skim milk...
May 2017: International Journal of Biological Macromolecules
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