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MFGM, lactoferrin

Kirsten Berding, Mei Wang, Marcia H Monaco, Lindsey S Alexander, Austin T Mudd, Maciej Chichlowski, Rosaline V Waworuntu, Brian M Berg, Michael J Miller, Ryan N Dilger, Sharon M Donovan
OBJECTIVE: This study tested the hypothesis that the addition of prebiotics and two functional milk ingredients to infant formula would maintain normal growth and gut development and modify microbiota composition and neurotransmitter gene expression in neonatal piglets. METHODS: Two-day-old male piglets (n = 24) were fed formula (CONT) or formula with polydextrose (PDX; 1.2 g/100 g diet), galactooligosaccharides (GOS; 3.5 g/100 g diet), bovine lactoferrin (Lf; 0...
March 23, 2016: Journal of Pediatric Gastroenterology and Nutrition
Austin T Mudd, Lindsey S Alexander, Kirsten Berding, Rosaline V Waworuntu, Brian M Berg, Sharon M Donovan, Ryan N Dilger
INTRODUCTION: Milk fat globule membrane (MFGM) and lactoferrin have been identified as two components that have potential to affect neurodevelopment. While concentrations of some MFGM constituents in infant formulas are within human milk range, they may not be present at optimal or clinically effective levels. However, lactoferrin levels of infant formulas are consistently reported to be lower than human milk. This study sought to provide a novel combination of prebiotics, bovine-derived MFGM, and lactoferrin and assess their influence on neurodevelopment...
2016: Frontiers in Pediatrics
Shunchao Sui, Jie Zhao, Jianwu Wang, Ran Zhang, Chengdong Guo, Tian Yu, Ning Li
The use of transgenic livestock is providing new methods for obtaining pharmaceutically useful proteins. However, the protein expression profiles of the transgenic animals, including expression of milk fat globule membrane (MFGM) proteins, have not been well characterized. In this study, we compared the MFGM protein expression profile of the colostrum and mature milk from three lines of transgenic cloned (TC) cattle, i.e., expressing recombinant human α-lactalbumin (TC-LA), lactoferrin (TC-LF) or lysozyme (TC-LZ) in the mammary gland, with those from cloned non-transgenic (C) and conventionally bred normal animals (N)...
2014: PloS One
J A Dickow, L B Larsen, M Hammershøj, L Wiking
Lipoprotein lipase (LPL) activity and free fatty acid levels were studied in freshly milked, uncooled milk from individual Danish Holstein or Jersey cows, or after storage for up to 24h at either a cooling temperature (4°C) or at the milking temperature (31°C). Upon cooling for up to 24h, LPL activity increased in the cream phase, whereas the activity in the skim milk was steady, as observed for Jersey cows, or increased, as seen for the Holsteins. Storage at 31°C decreased the LPL activity in both the cream phase and the skim milk phase...
February 2011: Journal of Dairy Science
M G Giuffrida, M Cavaletto, C Lamberti, G Dellavalle, C Fabris, A Conti, G Sabatino, T Testa, A Coscia, F Giuliani, E Bertino
Milk fat globule membrane (MFGM) proteins constitute a milk fraction currently of great interest, as they appear to significantly contribute to milk protective role. We investigated these proteins in human preterm colostrum and milk. For the former we found a peculiar 2-DE pattern, with a spot concentration at low molecular weight, which mass spectrometry analysis showed to be fragments belonging to some MFGM proteins with a well-known biological and especially immunological role: lactadherin, membrane-associated lactoferrin, butyrophilin, clusterin and heavy-chain immunoglobulin...
October 2008: International Journal of Immunopathology and Pharmacology
X Wang, S Hirmo, R Willén, T Wadström
The attachment of Helicobacter pylori to the human gastric mucosa is a complex process involving several specific structures recognised by the cell surface receptors. Sialylated multivalent high mol. wt glycoproteins have been shown to inhibit H. pylori sialic acid-specific haemagglutination. This study explored whether sialylated glycoconjugates from bovine milk could inhibit an experimental H. pylori infection in a mouse model. BALB/cA mice (6-8 weeks old) were inoculated with a mouse-passaged H. pylori strain 317p...
May 2001: Journal of Medical Microbiology
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