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https://www.readbyqxmd.com/read/27914650/towards-upright-pedalling-to-drive-recovery-in-people-who-cannot-walk-in-the-first-weeks-after-stroke-movement-patterns-and-measurement
#1
Nicola J Hancock, Lee Shepstone, Philip Rowe, Phyo K Myint, Valerie M Pomeroy
OBJECTIVES: To examine whether people who are within 31days of stroke onset are able to produce controlled lower limb movement, and phasic activity in antagonistic lower limb muscle groups, during Upright Pedalling (UP). DESIGN: Observational study. SETTING: Acute stroke unit within a University Hospital. PARTICIPANTS: Eight adults between 3 and 30days from stroke onset, with unilateral lower limb paresis and unable to walk without assistance...
November 15, 2016: Physiotherapy
https://www.readbyqxmd.com/read/27914204/the-genetic-architecture-of-tristyly-and-its-breakdown-to-self-fertilization
#2
Ramesh Arunkumar, Wei Wang, Stephen I Wright, Spencer C H Barrett
The floral polymorphism tristyly involves three style morphs with a reciprocal arrangement of stigma and anther heights governed by two diallelic loci (S and M). Tristyly functions to promote cross-pollination, but modifications to stamen position commonly cause transitions to selfing. Here, we integrate whole genome sequencing and genetic mapping to investigate the genetic architecture of the M locus and the genetic basis of independent transitions to selfing in tristylous Eichhornia paniculata. We crossed independently derived semi-homostylous selfing variants of the long- and mid-styled morph fixed for alternate alleles at the M locus (ssmm and ssMM, respectively) and backcrossed the F1 to the parental ssmm genotype...
December 3, 2016: Molecular Ecology
https://www.readbyqxmd.com/read/27913859/complex-genetics-architecture-contributes-to-salmonella-resistance-in-acb60-mice
#3
Sean Beatty, Leïla Rached-D'Astous, Danielle Malo
Human infection with Salmonella is of global public health concern. In low- and middle-income countries, Salmonella infection is a major source of disease in terms of both mortality and morbidity, while in high-income nations, the pathogen is an ongoing threat to food security. The outcome of infection with Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) in mouse models is dependent upon a coordinated and complex immune response. A panel of recombinant congenic strains (RCS) derived from the reciprocal double backcross of A/J and C57BL/6J mice has been screened for their susceptibility to Salmonella infection, and the RCS AcB60 was identified to be the most resistant strain to Salmonella infection, more resistant than the parental strain A/J...
December 2, 2016: Mammalian Genome: Official Journal of the International Mammalian Genome Society
https://www.readbyqxmd.com/read/27910655/a-fast-feedback-controlled-magnetic-drive-for-the-asdex-upgrade-fast-ion-loss-detectors
#4
J Ayllon-Guerola, J Gonzalez-Martin, M Garcia-Munoz, J Rivero-Rodriguez, A Herrmann, S Vorbrugg, P Leitenstern, S Zoletnik, J Galdon, J Garcia Lopez, M Rodriguez-Ramos, L Sanchis-Sanchez, A D Dominguez, M Kocan, J P Gunn, D Garcia-Vallejo, J Dominguez
A magnetically driven fast-ion loss detector system for the ASDEX Upgrade tokamak has been designed and will be presented here. The device is feedback controlled to adapt the detector head position to the heat load and physics requirements. Dynamic simulations have been performed taking into account effects such as friction, coil self-induction, and eddy currents. A real time positioning control algorithm to maximize the detector operational window has been developed. This algorithm considers dynamical behavior and mechanical resistance as well as measured and predicted thermal loads...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910499/conceptual-design-of-the-iter-fast-ion-loss-detector
#5
M Garcia-Munoz, M Kocan, J Ayllon-Guerola, L Bertalot, Y Bonnet, N Casal, J Galdon, J Garcia Lopez, T Giacomin, J Gonzalez-Martin, J P Gunn, M C Jimenez-Ramos, V Kiptily, S D Pinches, M Rodriguez-Ramos, R Reichle, J F Rivero-Rodriguez, L Sanchis-Sanchez, A Snicker, G Vayakis, E Veshchev, Ch Vorpahl, M Walsh, R Walton
A conceptual design of a reciprocating fast-ion loss detector for ITER has been developed and is presented here. Fast-ion orbit simulations in a 3D magnetic equilibrium and up-to-date first wall have been carried out to revise the measurement requirements for the lost alpha monitor in ITER. In agreement with recent observations, the simulations presented here suggest that a pitch-angle resolution of ∼5° might be necessary to identify the loss mechanisms. Synthetic measurements including realistic lost alpha-particle as well as neutron and gamma fluxes predict scintillator signal-to-noise levels measurable with standard light acquisition systems with the detector aperture at ∼11 cm outside of the diagnostic first wall...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27904771/tumor-suppressive-function-of-long-noncoding-rna-malat1-in-glioma-cells-by-suppressing-mir-155-expression-and-activating-fbxw7-function
#6
Shuanzhu Cao, Yanzhou Wang, Jinquan Li, Mingliang Lv, Haitao Niu, Yong Tian
The human metastasis associated lung adenocarcinoma transcript 1 (MALAT1) is a long non-coding RNA associated with metastasis, and is a favorable prognostic factor for lung cancer. Recent studies have shown that MALAT1 plays an important role in many malignancies. However, little is known about the role of MALAT1 in glioma. In this study, we determined the expression of MALAT1 and explored its prognostic value in glioma. Further, we investigated the regulatory mechanism of MALAT1 in glioma progression. Our results showed that the expression of MALAT1 was significantly decreased in glioma specimens than in noncancerous brain tissues...
2016: American Journal of Cancer Research
https://www.readbyqxmd.com/read/27902278/chryseobacterium-endophyticum-sp-nov-isolated-from-a-maize-leaf
#7
Shih-Yao Lin, Asif Hameed, You-Cheng Liu, Yi-Han Hsu, Yu-Ting Hsieh, Wei-An Lai, Chiu-Chung Young
A Gram-stain-negative, aerobic, yellow-pigmented, flexirubin-producing, rod-shaped and endophytic bacterium, designated strain CC-YTH209T, was isolated from a maize leaf and subjected to a taxonomic study. Strain CC-YTH209T was found to grow at 15-40 °C (optimal 30 °C), at pH 6.0-8.0 (optimal pH 7.0) and in the presence of 0-2 % (optimal 1 %) (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, strain CC-YTH209T appeared to belong to the genus Chryseobacterium within the class Flavobacteriia, and was closely related to Chryseobacterium rigui CJ16T (97...
November 11, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902214/brevibacillus-halotolerans-sp-nov-isolated-from-saline-soil-sample-collected-from-paddy-field
#8
Jinlong Song, Yanwei Wang, Yi Song, Bingqiang Zhao, Huimin Wang, Shan Zhou, Delong Kong, Xiang Guo, Yanting Li, Mingxiong He, Kedong Ma, Zhiyong Ruan, Yanchun Yan
Two novel aerobic bacteria, designated strains LAM0312T and LAM0313, were isolated from saline soil sample collected from paddy field in Dezhou city, Shandong Province in China. The Cells of these strains were Gram-stain positive, sporogenous, rod-shaped and motile with peritrichous flagella. The optimal growth temperature and pH were 30 ºC and pH 7.0-8.0, repesctively. Strain LAM0312T was found to be able to grow in the presence of 12 % (w/v) NaCl. The major fatty acids of strains were identified as anteiso-C15:0 and iso-C15:0...
October 17, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902186/multigenetic-characterization-of-candidatus-xenohaliotis-californiensis
#9
Francesco Cicala, James D Moore, Jorge Cáceres-Martínez, Miguel A Del Río-Portilla, Mónica Hernández-Rodríguez, Rebeca Vásquez-Yeomans, Axayácatl Rocha-Olivares
"Candidatus Xenohaliotis californiensis" (or Ca. Xc) is the etiological agent of the withering syndrome (WS), a chronic wasting disease affecting most if not all North American species of abalone, and has been described as a Rickettsiales-like prokaryote. Genetic data about this species is limited to the 16S rRNA gene. The inability to grow it axenically has hindered its genetic and genomic characterization and, in consequence, a thorough analysis of its systematics. Here, we amplified and sequenced five genes (16S rRNA, 23S rRNA, ftsZ, virD4, and virB11) of Ca...
October 11, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27895161/the-e-loop-of-the-transmitter-binding-site-is-a-key-determinant-of-the-modulatory-effects-of-physostigmine-on-neuronal-nicotinic-%C3%AE-4%C3%AE-2-receptors
#10
Xiaochun Jin, Megan M McCollum, Allison L Germann, Gustav Akk, Joseph H Steinbach
Physostigmine is a well-known inhibitor of acetylcholinesterase, which can also activate, potentiate and inhibit acetylcholine receptors, including neuronal nicotinic receptors comprising α4 and β2 subunits. We have found that the two stoichiometric forms of this receptor differ in the effects of physostigmine. The form containing 3 copies of α4 and 2 of β2 was potentiated at low concentrations of ACh and physostigmine, while the form containing 2 copies of α4 and 3 of β2 was inhibited. Chimeric constructs of subunits indicated that the presence of inhibition or potentiation depended on the source of the extracellular ligand-binding domain of the subunit...
November 28, 2016: Molecular Pharmacology
https://www.readbyqxmd.com/read/27893254/dynamic-interplay-between-merger-process-justice-and-cognitive-trust-in-top-management-a-longitudinal-study
#11
Janne Kaltiainen, Jukka Lipponen, Brian C Holtz
This study examines two fundamental concerns in the context of organizational change: employees' perceptions of merger process justice and cognitive trust in the top management team. Our main purpose is to better understand the nature of reciprocal relations between these important constructs through a significant change event. Previous research, building mainly on social exchange theory, has framed trust as a consequence of justice perceptions. More recently, scholars have suggested that this view may be overly simplistic and that trust-related cognitions may also represent an important antecedent of justice perceptions...
November 28, 2016: Journal of Applied Psychology
https://www.readbyqxmd.com/read/27893244/temporal-ordering-effects-of-adolescent-depression-relational-aggression-and-victimization-over-six-waves-fully-latent-reciprocal-effects-models
#12
Herbert W Marsh, Rhonda G Craven, Philip D Parker, Roberto H Parada, Jiesi Guo, Theresa Dicke, Adel Salah Abduljabbar
The temporal ordering of depression, aggression, and victimization has important implications for theory, policy, and practice. For a representative sample of high school students (Grades 7-10; N = 3,793) who completed the same psychometrically strong, multiitem scales 6 times over a 2-year period, there were reciprocal effects between relational-aggression and relational-victimization factors: aggression led to subsequent victimization and victimization led to subsequent aggression. After controlling for prior depression, aggression, and victimization, depression had a positive effect on subsequent victimization, but victimization had no effect on subsequent depression...
December 2016: Developmental Psychology
https://www.readbyqxmd.com/read/27890856/sirna-cell-penetrating-peptides-complexes-as-a-combinatorial-therapy-against-chronic-myeloid-leukemia-using-bv173-cell-line-as-model
#13
João Miguel Freire, Inês Rego de Figueiredo, Javier Valle, Ana Salomé Veiga, David Andreu, Francisco J Enguita, Miguel A R B Castanho
Chronic myeloid leukemia (CML) is a myeloproliferative disorder caused by a single gene mutation, a reciprocal translocation that originates the Bcr-Abl gene with constitutive tyrosine kinase activity. As a monogenic disease, it is an optimum target for RNA silencing therapy. We developed a siRNA-based therapeutic approach in which the siRNA is delivered by pepM or pepR, two cell-penetrating peptides (CPPs) derived from the dengue virus capsid protein. These peptides have a dual role: siRNA delivery into cells and direct action as bioportides, i...
November 24, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/27887565/genomic-evaluation-by-including-dominance-effects-and-inbreeding-depression-for-purebred-and-crossbred-performance-with-an-application-in-pigs
#14
Tao Xiang, Ole Fredslund Christensen, Zulma Gladis Vitezica, Andres Legarra
BACKGROUND: Improved performance of crossbred animals is partly due to heterosis. One of the major genetic bases of heterosis is dominance, but it is seldom used in pedigree-based genetic evaluation of livestock. Recently, a trivariate genomic best linear unbiased prediction (GBLUP) model including dominance was developed, which can distinguish purebreds from crossbred animals explicitly. The objectives of this study were: (1) methodological, to show that inclusion of marker-based inbreeding accounts for directional dominance and inbreeding depression in purebred and crossbred animals, to revisit variance components of additive and dominance genetic effects using this model, and to develop marker-based estimators of genetic correlations between purebred and crossbred animals and of correlations of allele substitution effects between breeds; (2) to evaluate the impact of accounting for dominance effects and inbreeding depression on predictive ability for total number of piglets born (TNB) in a pig dataset composed of two purebred populations and their crossbreds...
November 25, 2016: Genetics, Selection, Evolution: GSE
https://www.readbyqxmd.com/read/27885802/a-novel-p53-pomc-g%C3%AE-s-sash1-autoregulatory-feedback-loop-activates-mutated-sash1-to-cause-pathologic-hyperpigmentation
#15
Ding'an Zhou, Zhiyun Wei, Zhongshu Kuang, Huangchao Luo, Jiangshu Ma, Xing Zeng, Ke Wang, Beizhong Liu, Fang Gong, Jing Wang, Shanchuan Lei, Dongsheng Wang, Jiawei Zeng, Teng Wang, Yong He, Yongqiang Yuan, Hongying Dai, Lin He, Qinghe Xing
p53-Transcriptional-regulated proteins interact with a large number of other signal transduction pathways in the cell, and a number of positive and negative autoregulatory feedback loops act upon the p53 response. P53 directly controls the POMC/α-MSH productions induced by ultraviolet (UV) and is associated with UV-independent pathological pigmentation. When identifying the causative gene of dyschromatosis universalis hereditaria (DUH), we found three mutations encoding amino acid substitutions in the gene SAM and SH3 domain containing 1 (SASH1), and SASH1 was associated with guanine nucleotide-binding protein subunit-alpha isoforms short (Gαs)...
November 25, 2016: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/27884627/characterization-of-avian-paramyxovirus-serotype-14-a-novel-serotype-isolated-from-a-duck-fecal-sample-in-japan
#16
Rapeewan Thampaisarn, Vuong N Bui, Dai Q Trinh, Makoto Nagai, Tetsuya Mizutani, Tsutomu Omatsu, Yukie Katayama, Dulyatad Gronsang, Duong H T Le, Haruko Ogawa, Kunitoshi Imai
A hemagglutinating virus isolate designated 11OG0352, was obtained from a duck fecal sample. Genetic and virological analyses indicated that it might represent a novel serotype of avian paramyxovirus (APMV). Electron micrographs showed that the morphology of the virus particle was similar to that of APMV. The complete genome of this virus comprised 15,444 nucleotides complying with the paramyxovirus "rule of six" and contains six open reading frames (3'-N-P-M-F-HN-L-5'). The phylogenetic analysis of the whole genome revealed that the virus was a member of the genus Avulavirus, but that it was distinct from APMV-1 to APMV-13...
November 21, 2016: Virus Research
https://www.readbyqxmd.com/read/27881038/-enhance-her-pleasure-and-your-grip-strength-men-s-health-magazine-and-pseudo-reciprocal-pleasure
#17
Chelsey Nicole Porter, Nick Douglas, Martine Collumbien
This paper provides a snapshot of the Top Ten free, digital Men's Health magazine articles, accessed on a randomly selected day, that can be viewed as a collection; both a product for readership consumption and a construct of readership priorities. Through close textual analysis, we examine how discourses about masculinity, heterosex and consumerism have intersected to create a model of masculinity based on the discipline of male pleasure, which impacts on men's approach to female pleasure and gender dynamics...
November 24, 2016: Culture, Health & Sexuality
https://www.readbyqxmd.com/read/27878265/synergistic-effects-of-overexpression-of-bmp%C3%A2-2-and-tgf%C3%A2-%C3%AE-3-on-osteogenic-differentiation-of-bone-marrow-mesenchymal-stem-cells
#18
Yilin Wang, Tian He, Jie Liu, Hongzhi Liu, Lugang Zhou, Wei Hao, Yujie Sun, Xin Wang
Bone morphogenetic protein 2 (BMP-2) and transforming growth factor β (TGF-β) isoforms are important in advancing bone regeneration. The aim of the present study was to investigate the positive and reciprocal effect of TGF‑β3, one of the three TGF‑β isoforms, on BMP‑2 in promoting osteogenic differentiation. Exogenous BMP‑2 and TGF‑β3 genes were separately, and in combination, overexpressed in rabbit bone marrow‑derived mesenchymal stem cells (rBMSCs). Expression levels of BMP‑2 and TGF‑β3 were evaluated using reverse‑transcription‑polymerase chain reaction (RT‑PCR) and Western blotting assays...
November 22, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27871176/in-vivo-angiogenic-capacity-of-stem-cells-from-human-exfoliated-deciduous-teeth-with-human-umbilical-vein-endothelial-cells
#19
Ji-Hye Kim, Gee-Hye Kim, Jae-Won Kim, Hee Jang Pyeon, Jae Cheoun Lee, Gene Lee, Hyun Nam
Dental pulp is a highly vascularized tissue requiring adequate blood supply for successful regeneration. In this study, we investigated the functional role of stem cells from human exfoliated deciduous teeth (SHEDs) as a perivascular source for in vivo formation of vessel-like structures. Primarily isolated SHEDs showed mesenchymal stem cell (MSC)-like characteristics including the expression of surface antigens and in vitro osteogenic and adipogenic differentiation potentials. Moreover, SHEDs were positive for NG2, α-smooth muscle actin (SMA), platelet-derived growth factor receptor beta (PDGFRβ), and CD146 as pericyte markers...
November 30, 2016: Molecules and Cells
https://www.readbyqxmd.com/read/27868264/profiles-of-children-with-down-syndrome-who-meet-screening-criteria-for-autism-spectrum-disorder-asd-a-comparison-with-children-diagnosed-with-asd-attending-specialist-schools
#20
G Warner, P Howlin, E Salomone, J Moss, T Charman
BACKGROUND: Recent research suggests that around 16% to 18% of children with Down syndrome (DS) also meet diagnostic criteria for autism spectrum disorder (ASD). However, there are indications that profiles of autism symptoms in this group may vary from those typically described in children with ASD. METHOD: Rates of autism symptoms and emotional and behavioural problems among children with DS who screened positive for ASD on the Social Communication Questionnaire (SCQ) (n = 183) were compared with a group of children with clinical diagnoses of ASD (n = 189) attending specialist schools in the UK...
November 21, 2016: Journal of Intellectual Disability Research: JIDR
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