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https://www.readbyqxmd.com/read/29775205/modified-device-for-fluid-percussion-injury-in-rodents
#1
Wei Ouyang, Wenhui Wu, Zhiheng Fan, Jihui Wang, Huiju Pan, Weibin Yang
Fluid percussion (FP) injury model is a popular animal model of traumatic brain injury (TBI), but still there are some issues need to be addressed. To increase the validity and reliability of this technique, we adapted the FP device using electromagnetic protractor, stainless-steel cylinder, changing pressure transducer position, and foam pads to adjust the parameters of FP pulse. Besides, the adjusted FP device is more automatic. The FP pulse is promptly measured and displayed in a graphic user interface software...
May 18, 2018: Journal of Neuroscience Research
https://www.readbyqxmd.com/read/29766510/ineffectiveness-of-saxagliptin-as-a-neuroprotective-drug-in-6-ohda-lesioned-rats
#2
Joelle de Melo Turnes, Taysa Bervian Bassani, Leonardo C Souza, Maria A B F Vital
OBJECTIVES: To determine whether the drug saxagliptin, a dipeptidyl peptidase-4 (DPP-4) inhibitor which is utilized for the treatment of Diabetes Mellitus, has neuroprotective effects in the animal model of Parkinson's disease (PD) induced by 6-hydroxydopamine (6-OHDA) in rats. METHODS: Male Wistar rats (weighing 280-300 g) received a bilateral infusion of 6-OHDA in the substantia nigra. Twenty-four hours later, they were treated with saxagliptin (1 mg/kg, p.o) once daily, for 21 days...
May 16, 2018: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/29766026/development-of-intrathecal-aav9-gene-therapy-for-giant-axonal-neuropathy
#3
Rachel M Bailey, Diane Armao, Sahana Nagabhushan Kalburgi, Steven J Gray
An NIH-sponsored phase I clinical trial is underway to test a potential treatment for giant axonal neuropathy (GAN) using viral-mediated GAN gene replacement (https://clinicaltrials.gov/ct2/show/NCT02362438). This trial marks the first instance of intrathecal (IT) adeno-associated viral (AAV) gene transfer in humans. GAN is a rare pediatric neurodegenerative disorder caused by autosomal recessive loss-of-function mutations in the GAN gene, which encodes the gigaxonin protein. Gigaxonin is involved in the regulation, turnover, and degradation of intermediate filaments (IFs)...
June 15, 2018: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/29760428/generation-and-characterisation-of-a-parkin-pacrg-knockout-mouse-line-and-a-pacrg-knockout-mouse-line
#4
Sarah E M Stephenson, Timothy D Aumann, Juliet M Taylor, Jessica R Riseley, Ruili Li, Jeffrey R Mann, Doris Tomas, Paul J Lockhart
Mutations in PARK2 (parkin) can result in Parkinson's disease (PD). Parkin shares a bidirectional promoter with parkin coregulated gene (PACRG) and the transcriptional start sites are separated by only ~200 bp. Bidirectionally regulated genes have been shown to function in common biological pathways. Mice lacking parkin have largely failed to recapitulate the dopaminergic neuronal loss and movement impairments seen in individuals with parkin-mediated PD. We aimed to investigate the function of PACRG and test the hypothesis that parkin and PACRG function in a common pathway by generating and characterizing two novel knockout mouse lines harbouring loss of both parkin and Pacrg or Pacrg alone...
May 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29760195/neuron-specific-hur-deficient-mice-spontaneously-develop-motor-neuron-disease
#5
Kevin Sun, Xiao Li, Xing Chen, Ying Bai, Gao Zhou, Olga N Kokiko-Cochran, Bruce Lamb, Thomas A Hamilton, Ching-Yi Lin, Yu-Shang Lee, Tomasz Herjan
Human Ag R (HuR) is an RNA binding protein in the ELAVL protein family. To study the neuron-specific function of HuR, we generated inducible, neuron-specific HuR-deficient mice of both sexes. After tamoxifen-induced deletion of HuR, these mice developed a phenotype consisting of poor balance, decreased movement, and decreased strength. They performed significantly worse on the rotarod test compared with littermate control mice, indicating coordination deficiency. Using the grip-strength test, it was also determined that the forelimbs of neuron-specific HuR-deficient mice were much weaker than littermate control mice...
May 14, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29759134/behavioural-alterations-and-morphological-changes-are-attenuated-by-the-lack-of-trpa1-receptors-in-the-cuprizone-induced-demyelination-model-in-mice
#6
Kata Bölcskei, Gábor Kriszta, Éva Sághy, Maja Payrits, Éva Sipos, Anett Vranesics, Zoltán Berente, Hajnalka Ábrahám, Péter Ács, Sámuel Komoly, Erika Pintér
We have recently reported that the Transient Receptor Potential Ankyrin 1 (TRPA1) receptor deficiency significantly attenuated cuprizone-induced demyelination by reducing the apoptosis of mature oligodendrocytes. The aim of the present study was to gather additional data on the role of TRPA1 by investigating the time course of behavioural alterations and morphological changes in cuprizone-treated TRPA1 receptor gene-deficient mice. Demyelination was induced by feeding male wild-type (WT) and TRPA1 gene-deleted (TRPA1 KO) mice with 0...
July 15, 2018: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/29756519/adipose-derived-stem-cells-stimulated-with-n-butylidenephthalide-exhibit-therapeutic-effects-in-a-mouse-model-of-parkinson-s-disease
#7
Kang Chi, Ru-Huei Fu, Yu-Chuen Huang, Shih-Yin Chen, Ching-Ju Hsu, Shinn-Zong Lin, Chi-Tang Tu, Li-Hsun Chang, Ping-An Wu, Shih-Ping Liu
Parkinson's disease (PD) causes motor dysfunction and dopaminergic cell death. Drug treatments can effectively reduce symptoms but often cause unwanted side effects. Stem cell therapies using cell replacement or indirect beneficial secretomes have recently emerged as potential therapeutic strategies. Although various types of stem cells have been proposed as possible candidates, adipose-derived stem cells (ADSCs) are easily obtainable, more abundant, less ethically disputed, and able to differentiate into multiple cell lineages...
January 1, 2018: Cell Transplantation
https://www.readbyqxmd.com/read/29754274/neuroprotective-and-antinociceptive-effects-of-rosemary-rosmarinus-officinalis-l-extract-in-rats-with-painful-diabetic-neuropathy
#8
Bahram Rasoulian, Zahra Hajializadeh, Saeed Esmaeili-Mahani, Marzieh Rashidipour, Iman Fatemi, Ayat Kaeidi
Diabetes mellitus is associated with the development of neuronal tissue damage in different central and peripheral nervous system regions. A common complication of diabetes is painful diabetic peripheral neuropathy. We have explored the antihyperalgesic and neuroprotective properties of Rosmarinus officinalis L. extract (RE) in a rat model of streptozotocin (STZ)-induced diabetes. The nociceptive threshold and motor coordination of these diabetic rats was assessed using the tail-flick and rotarod treadmill tests, respectively...
May 12, 2018: Journal of Physiological Sciences: JPS
https://www.readbyqxmd.com/read/29753957/brainstem-blood-brain-barrier-disruption-using-focused-ultrasound-a-demonstration-of-feasibility-and-enhanced-doxorubicin-delivery
#9
Saira Alli, Carlyn A Figueiredo, Brian Golbourn, Nesrin Sabha, Megan Yijun Wu, Andrew Bondoc, Amanda Luck, Daniel Coluccia, Colin Maslink, Christian Smith, Heiko Wurdak, Kullervo Hynynen, Meaghan O'Reilly, James T Rutka
Magnetic Resonance Image-guided Focused Ultrasound (MRgFUS) has been used to achieve transient BBB opening without tissue injury. Delivery of a targeted ultrasonic wave causes an interaction between administered microbubbles and the capillary bed resulting in enhanced vessel permeability. The use of MRgFUS in the brainstem has not previously been shown but could provide value in the treatment of tumours such as Diffuse Intrinsic Pontine Glioma (DIPG) where the intact BBB has contributed to the limited success of chemotherapy...
May 10, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29752287/-rnaset2-knockout-rats-exhibit-hippocampal-neuropathology-and-deficits-in-memory
#10
Kerstin W Sinkevicius, Thomas R Morrison, Praveen Kulkarni, Martha K Caffrey Cagliostro, Sade Iriah, Samantha Malmberg, Julia Sabrick, Jennifer A Honeycutt, Kim L Askew, Malav Trivedi, Craig F Ferris
RNASET2 deficiency in humans is associated with infant cystic leukoencephalopathy, which causes psychomotor impairment, spasticity, and epilepsy. A zebrafish mutant model suggests that loss of RNASET2 function leads to neurodegeneration due to the accumulation of non-degraded RNA in the lysosomes. The goal of this study was to characterize the first rodent model of RNASET2 deficiency. The brains of 3- and 12-month-old RNaseT2 knockout rats were studied using multiple magnetic resonance imaging modalities and behavioral tests...
May 10, 2018: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/29743852/n1-benzenesulfonyl-2-pyrazoline-hybrids-in-neurological-disorders-syntheses-biological-screening-and-computational-studies
#11
Avinash C Tripathi, Savita Upadhyay, Sarvesh Paliwal, Shailendra K Saraf
A novel series of 1,3,5-trisubstituted-2-pyrazolines (5a-5t ) was prepared via Claisen Schmidt condensation, followed by heterocyclization with hydrazine hydrate, substitution of N1 hydrogen of 2-pyrazoline nucleus with 4-chlorobenzenesulfonylchloride, applying conventional and green chemistry approaches. Among the two, microwave assisted organic synthesis (MAOS) emerged as a better synthetic tool in terms of faster reaction rate and high yield. Various physicochemical and spectral studies were conducted to characterize the synthesized derivatives including- IR, Mass, 1 H-NMR, 13 C-NMR and elemental analysis...
2018: EXCLI Journal
https://www.readbyqxmd.com/read/29730027/synthesis-and-activity-of-di-or-trisubstituted-n-phenoxyalkyl-or-n-2-2-phenoxy-ethoxy-ethyl-piperazine-derivatives-on-the-central-nervous-system
#12
Katarzyna Pańczyk, Karolina Pytka, Magdalena Jakubczyk, Anna Rapacz, Kinga Sałat, Anna Furgała, Agata Siwek, Monika Głuch-Lutwin, Anna Gryboś, Karolina Słoczyńska, Elżbieta Pękala, Paweł Żmudzki, Adam Bucki, Marcin Kołaczkowski, Dorota Żelaszczyk, Henryk Marona, Anna M Waszkielewicz
Aim of the study was evaluation of anxiolytic, antidepressant, anticonvulsant and analgesic activity in a series of a consistent group of compounds. A series of eleven new N-(phenoxyalkyl)- or N-{2-[2-(phenoxy)ethoxy]ethyl}piperazine derivatives has been obtained. Their affinity towards 5-HT1A , 5-HT2A , 5-HT6 , 5-HT7 , D2 and α1 receptors has been assessed, and then functional assays were performed. The compounds were evaluated in mice, i.p. for their antidepressant-like (forced swim test), locomotor, anxiolytic-like (four-plate test) activities as well as - at higher doses - for anticonvulsant potential (MES) and neurotoxicity (rotarod)...
April 25, 2018: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29726751/evaluation-of-the-antiparkinsonism-and-neuroprotective-effects-of-hydrogen-sulfide-in-acute-6-hydroxydopamine-induced-animal-model-of-parkinson-s-disease-behavioral-histological-and-biochemical-studies
#13
Mohammad Sarukhani, Hashem Haghdoost-Yazdi, Ali Sarbazi Golezari, Arvin Babayan-Tazehkand, Tahere Dargahi, Nafiseh Rastgoo
INTRODUCTION: Studies have shown that hydrogen sulfide (H2 S), a gaseous neurotransmitter, has neuroprotective effect. Here, we evaluated the neuroprotective activity of H2 S in acute 6-hydroxydopamine (6-OHDA) animal model of Parkinson's disease (PD). METHODS: 6-OHDA was injected through stereotaxic surgery into medial forebrain bundle (MFB) of the right hemisphere to induce severe and fast degeneration in dopaminergic neurons of substantia nigra (SN). NaHS, as donor of H2 S, was daily injected at doses of 3 and 5...
May 4, 2018: Neurological Research
https://www.readbyqxmd.com/read/29725362/resveratrol-attenuates-neurological-deficit-and-neuroinflammation-following-intracerebral-hemorrhage
#14
Jia-Chen Cai, Wei Liu, Fei Lu, Wen-Bing Kong, Xin-Xuan Zhou, Peng Miao, Cheng-Xiang Lei, Yan Wang
Resveratrol (RESV) improves histopathological and behavioral outcomes in diseases of the central nervous system. However, to the best of our knowledge, there have been no studies investigating its neuroprotective effects on secondary brain injury following intracerebral hemorrhage (ICH). The aim of the present study was to evaluate the neuroprotective function of resveratrol following ICH. Male Sprague-Dawley rats were randomly divided into 3 groups: Sham, ICH and ICH+RESV groups. Rats underwent ICH and received an intraperitoneal injection of RESV daily...
May 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29715513/characterization-of-superparamagnetic-iron-oxide-nanoparticle-induced-apoptosis-in-pc12-cells-and-mouse-hippocampus-and-striatum
#15
Yutong Liu, Juan Li, Kaige Xu, Jingjing Gu, Lu Huang, Lei Zhang, N Liu, Jiming Kong, Malcolm Xing, Lin Zhang, Lu Zhang
Superparamagnetic iron oxide nanoparticles (SPIONs) have been widely used as theranostic drug-carrier and MRI contrast agent. Their potential effects are still in blank while SPIONs are used for brain. The present study aims to investigate SPIONs' neurotoxicity in vitro and in vivo using stereotaxic technique. By co-incubating SPIONs with dopaminergic neuronal PC12 cells, we found that SPIONs had a dose-dependent cytotoxic in PC12 cells at 60-200 ug/mL but not at 10-50 ug/mL, it reduced cell viability, decreased the capacity of PC12 cells to extend neurites in response to nerve growth factor (NGF), induced a reduction of the tyrosine hydroxylase protein, while increasing PC12 cell apoptosis...
April 28, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29702698/comprehensive-behavioral-analysis-of-the-cdkl5-knockout-mice-revealed-significant-enhancement-in-anxiety-and-fear-related-behaviors-and-impairment-in-both-acquisition-and-long-term-retention-of-spatial-reference-memory
#16
Kosuke Okuda, Keizo Takao, Aya Watanabe, Tsuyoshi Miyakawa, Masashi Mizuguchi, Teruyuki Tanaka
Mutations in the Cyclin-dependent kinase-like 5 (CDKL5) gene cause severe neurodevelopmental disorders. Recently we have generated Cdkl5 KO mice by targeting exon 2 on the C57BL/6N background, and demonstrated postsynaptic overaccumulation of GluN2B-containing N-methyl-D-aspartate (NMDA) receptors in the hippocampus. In the current study, we subjected the Cdkl5 KO mice to a battery of comprehensive behavioral tests, aiming to reveal the effects of loss of CDKL5 in a whole perspective of motor, emotional, social, and cognition/memory functions, and to identify its undetermined roles...
2018: PloS One
https://www.readbyqxmd.com/read/29697531/mice-lacking-kcns1-in-peripheral-neurons-show-increased-basal-and-neuropathic-pain-sensitivity
#17
Christoforos Tsantoulas, Franziska Denk, Massimo Signore, Mohammed A Nassar, Kensuke Futai, Stephen B McMahon
Voltage-gated potassium (Kv) channels are increasingly recognised as key regulators of nociceptive excitability. Kcns1 is one of the first potassium channels to be associated with neuronal hyperexcitability and mechanical sensitivity in the rat, as well as pain intensity and risk of developing chronic pain in humans. Here, we show that in mice Kcns1 is predominantly expressed in the cell body and axons of myelinated sensory neurons positive for neurofilament-200, including Aδ-fiber nociceptors and low-threshold Aβ mechanoreceptors...
April 24, 2018: Pain
https://www.readbyqxmd.com/read/29692268/cerebrolysin-and-aquaporin-4-inhibition-improve-pathological-and-motor-recovery-after-ischemic-stroke
#18
Bogdan Catalin, Ionica Pirici, Tudor Adrian Balseanu, Adina Stan, Valerica Tudorica, Maria Balea, Ion Mindrila, Carmen Valeria Albu, Guleed Mohamed, Daniel Pirici, Dafin Fior Muresanu
BACKGROUND: Edema represents one of the earliest negative markers of survival and consecutive neurological deficit following stroke. The mixture of cellular and vasogenic edema makes treating this condition complicated, and to date, there is no pathogenically oriented drug treatment for edema, which leaves parenteral administration of a hypertonic solution as the only non-surgical alternative. OBJECTIVE: New insights into water metabolism in the brain have opened the way for molecular targeted treatment, with aquaporin 4 channels (AQP4) taking center stage...
April 25, 2018: CNS & Neurological Disorders Drug Targets
https://www.readbyqxmd.com/read/29691318/control-of-motor-coordination-by-astrocytic-tonic-gaba-release-through-modulation-of-excitation-inhibition-balance-in-cerebellum
#19
Junsung Woo, Joo Ok Min, Dae-Si Kang, Yoo Sung Kim, Guk Hwa Jung, Hyun Jung Park, Sunpil Kim, Heeyoung An, Jea Known, Jeongyeon Kim, Insop Shim, Hyung-Gun Kim, C Justin Lee, Bo-Eun Yoon
Tonic inhibition in the brain is mediated through an activation of extrasynaptic GABAA receptors by the tonically released GABA, resulting in a persistent GABAergic inhibitory action. It is one of the key regulators for neuronal excitability, exerting a powerful action on excitation/inhibition balance. We have previously reported that astrocytic GABA, synthesized by monoamine oxidase B (MAOB), mediates tonic inhibition via GABA-permeable bestrophin 1 (Best1) channel in the cerebellum. However, the role of astrocytic GABA in regulating neuronal excitability, synaptic transmission, and cerebellar brain function has remained elusive...
April 24, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29689641/inducing-different-brain-injury-levels-using-shock-wave-lithotripsy
#20
Afshin A Divani, Pascal Salazar, Manoj Monga, Greg J Beilman, Karen S SantaCruz
OBJECTIVES: To assess the feasibility of inducing different severities of shock wave (SW)-induced traumatic brain injury (TBI) using lithotripsy. METHODS: Wistar rats (n = 36) were divided into 2 groups: group 1 (n = 20) received 5 SW pulses, and group 2 (n = 16) received 15 SWs pulses. The SW pulses were delivered to the right side of the frontal cortex. Neurologic and behavioral assessments (Garcia test, beam walking, rotarod, and elevated plus maze) were performed at the baseline and at 3, 6, 24, 72, and 168 hours after injury...
April 24, 2018: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
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