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https://www.readbyqxmd.com/read/28916171/neurobehavioral-assessment-of-mice-following-repeated-oral-exposures-to-domoic-acid-during-prenatal-development
#1
Motohiro Shiotani, Toby B Cole, Sungwoo Hong, Julie Ju Young Park, William C Griffith, Thomas M Burbacher, Tomomi Workman, Lucio G Costa, Elaine M Faustman
Domoic acid (DA) is an algal toxin which has been associated with significant neurotoxicity in humans, non-human primates, rodents, and marine mammals. Developmental exposure to DA is believed to result in neurotoxicity that may persist into adulthood. DA is produced by harmful algal blooms of Pseudo-nitzschia, raising concerns about the consumption of contaminated seafood. We evaluated oral exposures to DA during pregnancy in mice. Doses of 0 (vehicle), 1 or 3mg/kg/d of DA were administered by gavage to C57BL/6J mice on gestational days 10 to 17...
September 12, 2017: Neurotoxicology and Teratology
https://www.readbyqxmd.com/read/28914762/the-1-week-and-8-month-effects-of-a-ketogenic-diet-or-ketone-salt-supplementation-on-multi-organ-markers-of-oxidative-stress-and-mitochondrial-function-in-rats
#2
Wesley C Kephart, Petey W Mumford, Xuansong Mao, Matthew A Romero, Hayden W Hyatt, Yufeng Zhang, Christopher B Mobley, John C Quindry, Kaelin C Young, Darren T Beck, Jeffrey S Martin, Danielle J McCullough, Dominic P D'Agostino, Ryan P Lowery, Jacob M Wilson, Andreas N Kavazis, Michael D Roberts
We determined the short- and long-term effects of a ketogenic diet (KD) or ketone salt (KS) supplementation on multi-organ oxidative stress and mitochondrial markers. For short-term feedings, 4 month-old male rats were provided isocaloric amounts of KD (n = 10), standard chow (SC) (n = 10) or SC + KS (~1.2 g/day, n = 10). For long-term feedings, 4 month-old male rats were provided KD (n = 8), SC (n = 7) or SC + KS (n = 7) for 8 months and rotarod tested every 2 months. Blood, brain (whole cortex), liver and gastrocnemius muscle were harvested from all rats for biochemical analyses...
September 15, 2017: Nutrients
https://www.readbyqxmd.com/read/28913350/neuronal-regeneration-after-electroacupuncture-treatment-in-ischemia-reperfusion-injured-cerebral-infarction-rats
#3
Shiang-Lin Liao, Yi-Wen Lin, Ching-Liang Hsieh
Adult neuronal cells which can regenerate have been reported. The present study investigated whether acupuncture enhances neuronal regeneration in ischemic stroke rats. We established an ischemic stroke rat model by occluding the cerebral blood flow of the right middle cerebral artery for 15 minutes and then allowing reperfusion in Sprague-Dawley rats. The results indicated that, in these rats, 2 Hz electroacupuncture (EA) at both Zusanli (ST36) and Shangjuxu (ST37) acupoints reduced the infarction/hemisphere ratio 8 days after reperfusion and reduced the modified neurological severity score (mNSS) and increased the rotarod test time 4 and 8 days after reperfusion, respectively...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28901462/role-of-ghrelin-in-small-intestinal-motility-following-pediatric-intracerebral-hemorrhage-in-mice
#4
Jieyu Zan, Lei Song, Jiejie Wang, Rong Zou, Fei Hong, Jinhua Zhao, Yijun Cheng, Ming Xu
Small intestinal motility (SIM) disorder is a common complication following pediatric intracerebral hemorrhage (ICH), leading to a poor prognosis in patients. Previous studies have shown that ghrelin is involved in SIM in various diseases; however, the role of ghrelin in pediatric ICH‑induced SIM disorder remains to be elucidated. The present study was designed to investigate the association between ghrelin and SIM post‑ICH, and to examine the effect of exogenous ghrelin administration on SIM in vivo...
September 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28899730/a-green-tea-polyphenol-epigallocatechin-3-gallate-enhances-neuroregeneration-after-spinal-cord-injury-by-altering-levels-of-inflammatory-cytokines
#5
Lucia Machova Urdzikova, Jiri Ruzicka, Kristyna Karova, Anna Kloudova, Barbora Svobodova, Anubhav Amin, Jana Dubisova, Meic Schmidt, Sarka Kubinova, Meena Jhanwar-Uniyal, Pavla Jendelova
Spinal cord injury (SCI) is a debilitating condition which is characterized by an extended secondary injury due to the presence of inflammatory local milieu. Epigallocatechin gallate (EGCG) appears to possess strong neuroprotective properties. Here, we evaluated the beneficial effect of EGCG on recovery from SCI. Male Wistar rats were given either EGCG or saline directly to the injured spinal cord and thereafter a daily IP injection. Behavior recovery was monitored by BBB, plantar, rotarod and flat-beam tests...
September 9, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28895054/a-neuron-specific-gene-therapy-relieves-motor-deficits-in-pompe-disease-mice
#6
Ni-Chung Lee, Wuh-Liang Hwu, Shin-Ichi Muramatsu, Darin J Falk, Barry J Byrne, Chia-Hao Cheng, Nien-Chu Shih, Kai-Ling Chang, Li-Kai Tsai, Yin-Hsiu Chien
In Pompe disease, deficient lysosomal acid α-glucosidase (GAA) activity causes glycogen accumulation in the muscles, which leads to weakness, cardiomyopathy, and respiratory failure. Although glycogen accumulation also occurs in the nervous system, the burden of neurological deficits in Pompe disease remains obscure. In this study, a neuron-specific gene therapy was administered to Pompe mice through intracerebroventricular injection of a viral vector carrying a neuron-specific promoter. The results revealed that gene therapy increased GAA activity and decreased glycogen content in the brain and spinal cord but not in the muscles of Pompe mice...
September 11, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28890389/motor-function-deficits-in-the-12-month-old-female-5xfad-mouse-model-of-alzheimer-s-disease
#7
T P O'Leary, A Robertson, P H Chipman, V F Rafuse, R E Brown
Motor problems occur early in some patients with Alzheimer's disease (AD) and as the disease progresses many patients develop motor dysfunction. Motor dysfunction has been reported in some mouse models of AD, including the 5xFAD mouse, thus this model may be particularly useful for studying motor dysfunction in AD. In order to determine the extent of motor dysfunction in these mice, we tested 11-13 month old female 5xFAD and wildtype (WT) control mice in a battery of motor behaviour tasks. The 5xFAD mice showed hind limb clasping, weighed less and had slower righting reflexes than WT mice...
September 7, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/28884085/anti-anxiety-and-hypnotic-effects-of-ethanolic-and-aqueous-extracts-of-lippia-citriodora-leaves-and-verbascoside-in-mice
#8
Bibi Marjan Razavi, Naser Zargarani, Hossein Hosseinzadeh
OBJECTIVES: The extract of Lippia citriodora and its main component, verbascoside, are known for their hypnotic effects in traditional medicine. In this study, the anxiolytic and hypnotic effects of L. citriodora leave extracts and verbascoside were evaluated in mice. MATERIALS AND METHODS: Animals were divided into 11 groups of six mice each. Group I received normal saline, Group II received Diazepam (2 mg/kg) as positive control, Groups III, IV and V received L...
July 2017: Avicenna Journal of Phytomedicine
https://www.readbyqxmd.com/read/28872135/methods-and-tips-for-intravenous-administration-of-adeno-associated-virus-to-rats-and-evaluation-of-central-nervous-system-transduction
#9
Mychal S Grames, Kasey L Jackson, Robert D Dayton, John A Stanford, Ronald L Klein
Adeno-associated virus (AAV) vectors are a key reagent in the neurosciences for clustered regularly interspaced short palindromic repeats (CRISPR), optogenetics, cre-lox targeting, etc. The purpose of this manuscript is to aid the investigator attempting expansive central nervous system (CNS) gene transfer in the rat via tail vein injection of AAV. Wide-scale expression is relevant for conditions with widespread pathology, and a rat model is significant due to its greater size and physiologic similarities to humans compared to mice...
August 25, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28852397/neuroprotective-potential-of-quercetin-in-combination-with-piperine-against-1-methyl-4-phenyl-1-2-3-6-tetrahydropyridine-induced-neurotoxicity
#10
Shamsher Singh, Sumit Jamwal, Puneet Kumar
1-Methy-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is a neurotoxin that selectively damages dopaminergic neurons in the substantia nigra pars compacta and induces Parkinson's like symptoms in rodents. Quercetin (QC) is a natural polyphenolic bioflavonoid with potent antioxidant and anti-inflammatory properties but lacks of clinical attraction due to low oral bioavailability. Piperine is a well established bioavailability enhancer used pre-clinically to improve the bioavailability of antioxidants (e.g., Quercetin)...
July 2017: Neural Regeneration Research
https://www.readbyqxmd.com/read/28840056/effects-of-erythropoietin-on-gliogenesis-during-cerebral-ischemic-reperfusion-recovery-in-adult-mice
#11
Rongliang Wang, Jincheng Li, Yunxia Duan, Zhen Tao, Haiping Zhao, Yumin Luo
Erythropoietin (EPO) promotes oligodendrogenesis and attenuates white matter injury in neonatal rats. However, it is unknown whether this effect extends to adult mice and whether EPO regulate microglia polarization after ischemic stroke. Male adult C57BL/6 mice (25-30g) were subjected to 45 min of middle cerebral artery occlusion (MCAO). EPO (5000 IU/kg) or saline was injected intraperitoneally every other day after reperfusion. Neurological function was evaluated using the rotarod test at 1, 3, 7 and 14 days after MCAO...
July 2017: Aging and Disease
https://www.readbyqxmd.com/read/28836128/purinergic-antagonism-prevents-mitochondrial-dysfunction-and-behavioral-deficits-associated-with-dopaminergic-toxicity-induced-by-6-ohda-in-rats
#12
Saket Kumar, Akanksha Mishra, Sairam Krishnamurthy
Purinoceptors are present in neurons, microglia and oligodendrocytes and regulate dopamine (DA) release, striatal-related function and striatal neuronal and DA cells damage. Therefore, purinoceptors may be involved in the pathology of Parkinson's disease (PD) and purinergic antagonism may show neuroprotective effect. The study investigated the role of the non-selective purinergic receptor antagonist pyridoxalphosphate-6-azophenyl-2('), 4(')-disulfonic acid (PPADS) and a selective purinergic receptor P2X7 receptor antagonist Brilliant Blue G (BBG) against 6-OHDA induced dopaminergic neurotoxicity in rats; while adenosine triphosphate (ATP) was used as a P2X receptor agonist...
August 24, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28835392/epigallocatechin-gallate-has-a-neurorescue-effect-in-a-mouse-model-of-parkinson-disease
#13
Qi Xu, Monica Langley, Anumantha G Kanthasamy, Manju B Reddy
Background: Parkinson disease (PD) is a neurodegenerative disorder that has been associated with many factors, including oxidative stress, inflammation, and iron accumulation. The antioxidant, anti-inflammatory, and iron-chelating properties of epigallocatechin gallate (EGCG), a major polyphenol in green tea, may offer protection against PD.Objective: We sought to determine the neurorescue effects of EGCG and the role of iron in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD.Methods: We evaluated the neurorescue effect of EGCG (25 mg/kg, 7 d, oral administration) against MPTP-induced (20 mg/kg, 3 d, intraperitoneal injection) neurodegeneration in C57 male black mice...
August 23, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28821887/micrornas-218a-5p-219a-5p-and-221-3p-regulate-vestibular-compensation
#14
Mun Young Chang, Sohyeon Park, Jun Jae Choi, Young-Kook Kim, Myung-Whan Suh, Jun Ho Lee, Seung Ha Oh, Moo Kyun Park
Unilateral vestibular deafferentation (UVD) interrupts afferent signals from one side, resulting in an imbalance of the resting activity between bilateral vestibular nuclei. Vestibular compensation is the process of balancing the resting activity to reestablish homeostasis. Here, we investigated microRNAs (miRNAs) that regulate vestibular compensation using the Sprague-Dawley rat. After determining the progression of vestibular compensation following UVD, microarray analysis was performed and nine miRNAs were selected as candidates...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28818411/long-term-supplementation-with-egcg-and-beta-alanine-decreases-mortality-but-does-not-affect-cognitive-or-muscle-function-in-aged-mice
#15
Brandt D Pence, Tushar K Bhattacharya, Pul Park, Jennifer L Rytych, Jacob M Allen, Yi Sun, Robert H McCusker, Keith W Kelley, Rodney W Johnson, Justin S Rhodes, Jeffrey A Woods
We have previously shown that 6weeks of a diet containing epigallocatechin gallate (EGCG) and beta-alanine (B-ALA) was not effective in improving either cognitive or muscle function in aged (18month) mice (Gibbons et al. Behav Brain Res 2014). However, diet reduced oxidative stress in the brain, and previous studies using longer-term interventions have documented beneficial effects in cognitive, but not muscle, function. Therefore, we investigated the effect of 6months of feeding on measures of cognitive and muscle function in mice...
August 14, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/28806600/design-synthesis-and-evaluation-of-newer-5-6-dihydropyrimidine-2-1h-thiones-as-gaba-at-inhibitors-for-anticonvulsant-potential
#16
Meeta Sahu, Nadeem Siddiqui, Ramsha Iqbal, Vidushi Sharma, Sharad Wakode
Several new 5,6-dihydropyrimidine-2(1H)-thione derivatives have been prepared and investigated for their potencies for anticonvulsant activity against maximal electroshock (MES) and subcutaneous pentylenetetrazole (scPTZ) test in mice. The acute neurotoxicity was measured by rotarod test. Compounds 3c and 3l were found active in both of the animal models. Further, in vitro GABA-AT enzyme activity assay was carried out to investigate the possible mechanism of action through GABA-AT inhibition. The most potent compounds 3c and 3l showed inhibitory potency (IC50) of 18...
July 28, 2017: Bioorganic Chemistry
https://www.readbyqxmd.com/read/28805764/-behavioral-phenotyping-in-rats-during-the-experimental-modeling-of-chronic-cerebral-circulation-disorder
#17
D V Kurkin, E I Morkovin, D V Verkholiak, N A Osadchenko, I N Tyurenkov, V I Petrov
AIM: To describe motor, adaptive and cognitive disorders in rats with chronic cerebral circulatory deficiency caused by partial stenosis of the common carotid arteries (CCA). MATERIAL AND METHODS: A study was performed on 20 white outbred male rats. This manipulation led to 40-45% and 50-60% reduction of blood flow in CCA and in the brain, respectively. Twenty days after operation, animal's condition was assessed in the following tests: open field test, rotarod performance test, marble burying test and novel object recognition...
2017: Zhurnal Nevrologii i Psikhiatrii Imeni S.S. Korsakova
https://www.readbyqxmd.com/read/28803956/selective-cholinergic-depletion-of-pedunculopontine-tegmental-nucleus-aggravates-freezing-of-gait-in-parkinsonian-rats
#18
Hu Xiao, Min Li, Junbin Cai, Nanxiang Li, Ming Zhou, Peng Wen, Zhengyuan Xie, Qiang Wang, Jingyu Chang, Wangming Zhang
Many patients of advanced Parkinson's disease (PD) suffer from intractable axial symptoms (severe gait and postural impairments), which were recently speculated to be more relevant to cholinergic degeneration in the brainstem than dopaminergic degeneration in the substantia nigra compacta (SNc). To investigate the role of the cholinergic cells of the pedunculopontine tegmental nucleus (PPTg) on motor deficits, especially the axial motor impairments, we measured and analyzed the gait performance of sham lesion rats, SNc dopaminergic lesion rats, PPTg cholinergic lesion rats, and combined lesion rats by using the CatWalk system...
August 10, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28800740/neurocognitive-sparing-of-desktop-microbeam-irradiation
#19
Soha Bazyar, Christina R Inscoe, Thad Benefield, Lei Zhang, Jianping Lu, Otto Zhou, Yueh Z Lee
BACKGROUND: Normal tissue toxicity is the dose-limiting side effect of radiotherapy. Spatial fractionation irradiation techniques, like microbeam radiotherapy (MRT), have shown promising results in sparing the normal brain tissue. Most MRT studies have been conducted at synchrotron facilities. With the aim to make this promising treatment more available, we have built the first desktop image-guided MRT device based on carbon nanotube x-ray technology. In the current study, our purpose was to evaluate the effects of MRT on the rodent normal brain tissue using our device and compare it with the effect of the integrated equivalent homogenous dose...
August 11, 2017: Radiation Oncology
https://www.readbyqxmd.com/read/28795363/fibroblast-growth-factor-type-1-fgf1-overexpressed-adipose-derived-mesenchaymal-stem-cells-ad-msc-fgf1-induce-neuroprotection-and-functional-recovery-in-a-rat-stroke-model
#20
Hamed Ghazavi, Seyed Javad Hoseini, Alireza Ebrahimzadeh-Bideskan, Baratali Mashkani, Soghra Mehri, Ahmad Ghorbani, Kayvan Sadri, Elahe Mahdipour, Faezeh Ghasemi, Fatemeh Forouzanfar, Azar Hoseini, Ali Reza Pasdar, Hamid Reza Sadeghnia, Majid Ghayour-Mobarhan
Stroke, as the second most common cause of death, imposes a great financial burden on both the individual and society. Mesenchymal stem cells from rodents have demonstrated efficacy in experimental animal models of stroke due to enhanced neurological recovery. Since FGF1 (fibroblast growth factor 1) displays neuroprotective properties, for the first time, we investigated the effect of acute intravenous administration of FGF1 gene transfected adipose-derived mesenchymal stem cell (AD-MSC(FGF1)) on transient experimental ischemic stroke in rats...
August 9, 2017: Stem Cell Reviews
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