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https://www.readbyqxmd.com/read/28319902/ultra-high-sensitivity-of-the-non-immunological-affinity-of-graphene-oxide-peptide-based-surface-plasmon-resonance-biosensors-to-detect-human-chorionic-gonadotropin
#1
Nan-Fu Chiu, Chia-Tzu Kuo, Ting-Li Lin, Chia-Chen Chang, Chen-Yu Chen
Specific peptide aptamers can be used in place of expensive antibody proteins, and they are gaining increasing importance as sensing probes due to their potential in the development of non-immunological assays with high sensitivity, affinity and specificity for human chorionic gonadotropin (hCG) protein. We combined graphene oxide (GO) sheets with a specific peptide aptamer to create a novel, simple and label-free tool to detect abnormalities at an early stage of pregnancy, a GO-peptide-based surface plasmon resonance (SPR) biosensor...
March 7, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28318240/ppm-detection-of-alcohol-vapors-via-metal-organic-framework-functionalized-surface-plasmon-resonance-sensors
#2
Wouter Vandezande, Kris P F Janssen, Filip Delport, Rob Ameloot, Dirk E De Vos, Jeroen Lammertyn, Maarten B J Roeffaers
The development of novel molecular sieves opens opportunities in the development of more sensitive analytical devices. In this paper, metal organic frameworks (MOFs), specifically ZIF-8 and ZIF-93, are grown on fiber optic based surface plasmon resonance (FO-SPR) sensors. FO-SPR has enabled sensitive sensing capabilities in biomedical settings and the addition of a MOF coating opens the way for the sensing of volatile organic compounds (VOCs) in gaseous media. FO-SPR probes were homogeneously functionalized with ZIF-8 and ZIF-93 in each case using two different precursor solutions to obtain a sequential nucleation and growth phase...
March 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28316233/dna-linkers-and-diluents-for-ultrastable-gold-nanoparticle-bioconjugates-in-multiplexed-assay-development
#3
Samuel S Hinman, Kristy S McKeating, Quan Cheng
A novel bioconjugation strategy leading to ultrastable gold nanoparticles (AuNPs), utilizing DNA linkers and diluents in place of traditional self-assembled monolayers, is reported. The protective capacity of DNA confers straightforward biomolecular attachment and multi-step derivatization capabilities to these nanoparticles, and more significantly, substantially enhances their stability in demanding and complex sensing environments. The DNA/AuNPs were assembled through pH-assisted thiol-gold bonding of single stranded DNA and salt aging, with pre-conjugated biotin moieties facing outward from the gold surface...
March 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28302407/immunogenicity-and-safety-of-an-as03-adjuvanted-h7n1-vaccine-in-adults-65years-of-age-and-older-a-phase-ii-observer-blind-randomized-controlled-trial
#4
Anuradha Madan, Murdo Ferguson, Paul Rheault, David Seiden, Azhar Toma, Damien Friel, Jyoti Soni, Ping Li, Bruce L Innis, Anne Schuind
BACKGROUND: H7 influenza strains can cause severe and often fatal human infections, especially in the elderly. This phase II, observer-blind, randomized trial (www.ClinicalTrials.gov: NCT01949090) assessed the immunogenicity and safety of a novel AS03-adjuvanted H7N1 vaccine that may serve as a model H7-subtype vaccine. METHODS: 360 adults ≥65years of age in stable health received either 1 of 4 adjuvanted A/mallard/Netherlands/12/2000 split virion vaccine formulations (3...
March 13, 2017: Vaccine
https://www.readbyqxmd.com/read/28300766/an-improved-label-free-indirect-competitive-spr-immunosensor-and-its-comparison-with-conventional-elisa-for-ractopamine-detection-in-swine-urine
#5
Sai Wang, Shuai Zhao, Xiao Wei, Shan Zhang, Jiahui Liu, Yiyang Dong
Ractopamine (RCT) is banned for use in animals in many countries, and it is urgent to develop efficient methods for specific and sensitive RCT detection. A label-free indirect competitive surface plasmon resonance (SPR) immunosensor was first developed with a primary antibody herein and then improved by a secondary antibody for the detection of RCT residue in swine urine. Meanwhile, a pre-incubation process of RCT and the primary antibody was performed to further improve the sensitivity. With all the key parameters optimized, the improved immunosenor can attain a linear range of 0...
March 16, 2017: Sensors
https://www.readbyqxmd.com/read/28299701/aptasensors-for-detection-of-avian-influenza-virus-h5n1
#6
Yanbin Li, Ronghui Wang
Aptamers are single-stranded RNA or DNA oligonucleotides which have specific three-dimensional (3D) structures for high affinity and specific recognition to their target. In diagnostic and detection assays, aptamers represent an alternative to antibodies as recognition agents and offer advantages, such as reduced cost, rapid and reproducible synthesis, controllable modification, and improved stability. Aptamers are favorably used in biosensors as sensitive and selective bio-receptors coupled with a variety of transducers such as optical, mass-sensitive, and electrochemical sensors, the so-called aptasensors...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28299523/predictors-for-spontaneous-pregnancy-after-microsurgical-subinguinal-varicocelectomy-a-prospective-cohort-study
#7
Jian-Wei Zhang, Quan-Quan Xu, You-Lin Kuang, Yan Wang, Feng Xu, Yu-Dong Tian
PURPOSE: The purpose of this study is to determine the possible preoperative predictors of spontaneous pregnancy (SPR) for infertile males with varicocele after microsurgical subinguinal varicocelectomy (MVL) performed in two medical centers in a prospective cohort study. METHODS: A total of 120 males with varicocele that underwent MVL between June 2013 and June 2014 in two medical centers were documented. Related data, including male and female partner age, male body mass index (BMI), female BMI, preoperative semen parameters, hormone levels, testicular volume, grade and side of varicocele, were collected and analyzed...
March 15, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28297651/glycan-determinants-of-heparin-tau-interaction
#8
Jing Zhao, Isabelle Huvent, Guy Lippens, David Eliezer, Anqiang Zhang, Quanhong Li, Peter Tessier, Robert J Linhardt, Fuming Zhang, Chunyu Wang
Tau aggregates into paired helical filaments within neurons, a pathological hallmark of Alzheimer's disease. Heparin promotes tau aggregation and recently has been shown to be involved in the cellular uptake of tau aggregates. Although the tau-heparin interaction has been extensively studied, little is known about the glycan determinants of this interaction. Here, we used surface plasmon resonance (SPR) and NMR spectroscopy to characterize the interaction between two tau fragments, K18 and K19, and several polysaccharides, including heparin, heparin oligosaccharides, chemically modified heparin, and related glycans...
March 14, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28295434/the-potential-of-dual-energy-ct-to-reduce-proton-beam-range-uncertainties
#9
Esther Bär, Arthur Lalonde, Gary Royle, Hsiao-Ming Lu, Hugo Bouchard
PURPOSE: Dual-energy CT (DECT) promises improvements in estimating stopping power ratios (SPRs) for proton therapy treatment planning. Although several comparable mathematical formalisms have been proposed in literature, the optimal techniques to characterize human tissue SPRs with DECT in a clinical environment are not fully established. The aim of this work is to compare the most robust DECT methods against conventional single-energy CT (SECT) in conditions reproducing a clinical environment, where CT artifacts and noise play a major role on the accuracy of these techniques...
March 15, 2017: Medical Physics
https://www.readbyqxmd.com/read/28294502/the-role-of-anion-adsorption-in-the-effect-of-electrode-potential-on-spr-response
#10
Veronika K Laurinavichyute, Shavkat Nizamov, Vladimir M Mirsky
Surface plasmon resonance, being widely used in bioanalytics and biotechnology, is influenced by electrical potential of the resonant gold layer. To evaluate the mechanism of this effect, we have studied it in the solution of various inorganic electrolytes. The magnitude of the effect was decreased according to the series: KBr > KCl > KF > NaClO4. The data were treated using different models of the interface. Quantitative description was obtained for the model which takes into account local dielectric function of gold affected by free electron charge, diffuse ionic layer near gold / water interface and specific adsorption of halides to the gold surface with partial charge transfer...
March 11, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28288377/properties-of-modified-surface-for-biosensing-interface
#11
Mutsuo Tanaka, Takahiro Sawaguchi, Yoshiki Hirata, Osamu Niwa, Keiko Tawa, Chisato Sasakawa, Koji Kuraoka
Properties of modified surface, behavior against salting-out effect, suppressive effect for protein nonspecific adsorption, and wettability were examined using various mercapto compounds bearing methyloligoethylene glycol, oligoethylene glycol, alkyl oligoethylene glycol, alkyl phosphoryl choline, alkyl inverse phosphoryl choline, and alkyl sulfobetaine moieties. The behavior against salting-out effect was examined using gold nanoparticle with PBS and NaCl aqueous solution. The suppressive effect for protein nonspecific adsorption was evaluated by SPR, and the wettability was measured on the SPR chip...
March 2, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28288347/exploration-of-the-metal-coordination-region-of-concanavalin-a-for-its-interaction-with-human-norovirus
#12
Duwoon Kim, Hee-Min Lee, Kyung-Seo Oh, Ah Young Ki, Rachael A Protzman, Dongkyun Kim, Jong-Soon Choi, Min Ji Kim, Sung Hyun Kim, Bipin Vaidya, Seung Jae Lee, Joseph Kwon
Rapid methods for the detection and clinical treatment of human norovirus (HuNoV) are needed to control foodborne disease outbreaks, but reliable techniques that are fast and sensitive enough to detect small amounts of HuNoV in food and aquatic environments are not yet available. We explore the interactions between HuNoV and concanavalin A (Con A), which could facilitate the development of a sensitive detection tool for HuNoV. Biophysical studies including hydrogen/deuterium exchange (HDX) mass spectrometry and surface plasmon resonance (SPR) revealed that when the metal coordinated region of Con A, which spans Asp16 to His24, is converted to nine alanine residues (mCon A(MCR)), the affinity for HuNoV (GII...
March 6, 2017: Biomaterials
https://www.readbyqxmd.com/read/28287713/protein-adsorption-tailors-the-surface-energies-and-compatibility-between-polylactide-and-cellulose-nanofibrils
#13
Alexey Khakalo, Ilari Filpponen, Orlando J Rojas
The state of dispersion and the interactions between a polymer and a filler in a nanocomposite crucially define its properties and performance. The affinity of polylactide (PLA) with vegetable and animal proteins (casein, gelatin, soy protein isolate and hydrolysate) is investigated and their role as eco-friendly dispersants and compatibilizers of cellulose nanofibrils (CNF) is elucidated. The affinity of the proteins with PLA is determined by using sensograms acquired by electroacoustic (quartz crystal microgravimetry, QCM) and optical (surface plasmon resonance, SPR) techniques...
March 13, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28287708/lignin-films-from-spruce-eucalyptus-and-wheat-straw-studied-with-electroacoustic-and-optical-sensors-effect-of-composition-and-electrostatic-screening-on-enzyme-binding
#14
Antonio Pereira, Ingrid C Hoeger, Ana Ferrer Carrera, Jorge Rencoret, Jose Carlos Del Rio, Kristiina Kruus, Jenni Rahikainen, Miriam Kellock, Ana Gutierrez, Orlando J Rojas
Lignins were isolated from spruce, wheat straw and eucalyptus by using the milled wood lignin (MWL) method. Functional groups and compositional analyses were assessed via 2D NMR and 31P to realize their effect on enzyme binding. Films of the lignins were fabricated and ellipsometry, atomic force microscopy and water contact angle measurements were used for their characterization and to reveal the changes upon enzyme adsorption. Moreover, lignin thin films were deposited on quartz crystal microgravimetry (QCM) and surface plasmon (SPR) resonance sensors and used to gain further insights into the lignin-cellulase interactions...
March 13, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28287081/surface-plasmon-resonance-biosensor-for-enzymatic-detection-of-small-analytes
#15
Celina Massumi Miyazaki, Flávio Makoto Shimizu, J R Mejía-Salazar, Osvaldo N Oliveira, Marystela Ferreira
Surface plasmon resonance (SPR) biosensing is based on the detection of small changes in the refractive index on a gold surface modified with molecular recognition materials, thus being mostly limited to detecting large molecules. In this paper, we report on a SPR biosensing platform suitable to detect small molecules by making use of the mediator-type enzyme microperoxidase-11 (MP11) in layer-by-layer films. By depositing a top layer of glucose oxidase or uricase, we were able to detect glucose or uric acid with limits of detection of 3...
April 7, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28286323/switchsense-a-new-technology-to-study-protein-rna-interactions
#16
Antoine Cléry, Thibault J M Sohier, Thomas Welte, Andreas Langer, Frédéric H T Allain
Characterization of RNA-binding protein interactions with RNA became inevitable to properly understand the cellular mechanisms involved in gene expression regulation. Structural investigations bring information at the atomic level on these interactions and complementary methods such as Isothermal Titration Calorimetry (ITC) and Surface Plasmon Resonance (SPR) are commonly used to quantify the affinity of these RNA-protein complexes and evaluate the effect of mutations affecting these interactions. The switchSENSE technology has recently been developed and already successfully used to investigate protein interactions with different types of binding partners (DNA, protein/peptide or even small molecules)...
March 9, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28283728/survey-of-gadolinium-based-contrast-agent-utilization-among-the-members-of-the-society-for-pediatric-radiology-a-quality-and-safety-committee-report
#17
Einat Blumfield, Michael M Moore, Mary K Drake, Thomas R Goodman, Kristopher N Lewis, Laura T Meyer, Thang D Ngo, Christina Sammet, Arta Luana Stanescu, David W Swenson, Thomas L Slovis, Ramesh S Iyer
BACKGROUND: Gadolinium-based contrast agents (GBCAs) have been used for magnetic resonance (MR) imaging over the last three decades. Recent reports demonstrated gadolinium retention in patients' brains following intravenous administration. Since gadolinium is a highly toxic heavy metal, there is a potential for adverse effects from prolonged retention or deposition, particularly in children. For this reason, the Society (SPR) for Pediatric Radiology Quality and Safety committee conducted a survey to evaluate the current status of GBCAs usage among pediatric radiologists...
March 10, 2017: Pediatric Radiology
https://www.readbyqxmd.com/read/28281254/gold-nanorod-array-biochip-for-label-free-multiplexed-biological-detection
#18
Zhong Mei, Yanyan Wang, Liang Tang
Gold nanorod (GNR) based label-free sensing has been attractive due to its unique property of localized surface plasmon resonance (LSPR). Compared to bulk gold, the SPR of GNRs is more sensitive to the refractive index change caused by biological binding in the close proximity. Numerous studies have reported biological detection in solution based GNR probes. However, the biosensing has the intrinsic problems of fluctuating readings and short storage time due to nanoparticle aggregation. In contrast, a chip-based nanorod biosensor is a more robust and reliable platform...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28281253/microrna-biosensing-with-two-dimensional-surface-plasmon-resonance-imaging
#19
Ho Pui Ho, Fong Chuen Loo, Shu Yuen Wu, Dayong Gu, Ken-Tye Yong, Siu Kai Kong
Two-dimensional surface plasmon resonance (2D-SPR) imaging, which provides a real-time, sensitive, and high-throughput analysis of surface events in a two dimensional manner, is a valuable tool for studying biomolecular interactions and biochemical reactions without using any tag labels. The sensing principle of 2D-SPR includes angular, wavelength, and phase interrogation. In this chapter, the 2D-SPR imaging technique is applied for sensing a target microRNA by its corresponding oligonucleotide probes, with sequence complementarity, immobilized on the gold SPR sensing surface...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28281250/biosensing-based-on-magneto-optical-surface-plasmon-resonance
#20
Sorin David, Cristina Polonschii, Mihaela Gheorghiu, Dumitru Bratu, Eugen Gheorghiu
In spite of the high analytic potential of Magneto Optical Surface Plasmon Resonance (MOSPR) assays, their applicability to biosensing has been limited due to significant chip stability issues. We present novel solutions to surpass current limitations of MOSPR sensing assays, based on innovative chip structure, tailored measurements and improved data analysis methods. The structure of the chip is modified to contain a thin layer of Co-Au alloy instead of successive layers of homogenous metals with magnetic and plasmonic properties, as currently used...
2017: Methods in Molecular Biology
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