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https://www.readbyqxmd.com/read/28221377/the-nonmonotonous-shift-of-quantum-plasmon-resonance-and-plasmon-enhanced-photocatalytic-activity-of-gold-nanoparticles
#1
Si-Jing Ding, Da-Jie Yang, Jin-Ling Li, Gui-Ming Pan, Liang Ma, Yong-Jie Lin, Jia-Hong Wang, Li Zhou, Min Feng, Hongxing Xu, Shiwu Gao, Qu-Quan Wang
The surface plasmon resonance (SPR) of metal nanoparticles exhibits quantum behaviors as the size decreases owing to the transitions of quantized conduction electrons, but most studies are limited to the monotonous SPR blue-shift caused by off-resonant transitions. Here, we demonstrate the nonmonotonous SPR red-shift caused by resonant electron transitions and photocatalytic activity enhanced by the quantum plasmon resonance of colloidal gold nanoparticles. A maximal SPR wavelength and the largest photocatalytic activity are observed in the quantum regime for the first time for the gold nanoparticles with a diameter of 3...
February 21, 2017: Nanoscale
https://www.readbyqxmd.com/read/28220417/steady-state-and-kinetics-based-affinity-determination-in-effector-effector-target-interactions
#2
André Reinhard, Thorsten Nürnberger
Dissecting the functional basis of pathogenicity and resistance in the context of plant innate immunity benefits greatly from detailed knowledge about biomolecular interactions, as both resistance and virulence depend on specific interactions between pathogen and host biomolecules. While in vivo systems provide biological context to host-pathogen interactions, these experiments typically cannot provide quantitative biochemical characterization of biomolecular interactions. However, in many cases, the biological function does not only depend on whether an interaction occurs at all, but rather on the "intensity" of the interaction, as quantified by affinity...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28216573/a-conjugate-based-on-anti-her2-diaffibody-and-auristatin-e-targets-her2-positive-cancer-cells
#3
Anna M Serwotka-Suszczak, Alicja M Sochaj-Gregorczyk, Jerzy Pieczykolan, Daniel Krowarsch, Filip Jelen, Jacek Otlewski
Antibody-drug conjugates (ADCs) have recently emerged as efficient and selective cancer treatment therapeutics. Currently, alternative forms of drug carriers that can replace monoclonal antibodies are under intensive investigation. Here, a cytotoxic conjugate of an anti-HER2 (Human Epidermal Growth Factor Receptor 2) diaffibody with monomethyl-auristatin E (MMAE) is proposed as a potential anticancer therapeutic. The anti-HER2 diaffibody was based on the ZHER2:4 affibody amino acid sequence. The anti-HER2 diaffibody has been expressed as a His-tagged protein in E...
February 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28214745/rapid-detection-and-quantification-of-enterovirus-71-by-a-portable-surface-plasmon-resonance-biosensor
#4
Briliant Adhi Prabowo, Robert Y L Wang, Muhammad Khari Secario, Po-Ting Ou, Azharul Alom, Jia-Jung Liu, Kou-Chen Liu
This study presents the first report on a label-free detection and rapid quantification method for human enterovirus 71 (EV71) using a portable surface plasmon resonance (SPR) system. The SPR sensor instrument was configured to run on low power in a miniaturized platform to improve the device portability for a wider application both in laboratories and in the field. A color tunable organic light emitting diode in red spectrum was attached on a trapezoidal prism for the disposable light source module. The SPR signal processing using integration area under the reflectivity curve is applied for optimum signal to noise ratio (SNR) enhancement...
January 21, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28209997/antisense-mir-132-blockade-via-the-ache-r-splice-variant-mitigates-cortical-inflammation
#5
Nibha Mishra, Lyndon Friedson, Geula Hanin, Uriya Bekenstein, Meshi Volovich, Estelle R Bennett, David S Greenberg, Hermona Soreq
MicroRNA (miR)-132 brain-to-body messages suppress inflammation by targeting acetylcholinesterase (AChE), but the target specificity of 3'-AChE splice variants and the signaling pathways involved remain unknown. Using surface plasmon resonance (SPR), we identified preferential miR-132 targeting of soluble AChE-R over synaptic-bound AChE-S, potentiating miR-132-mediated brain and body cholinergic suppression of pro-inflammatory cytokines. Inversely, bacterial lipopolysaccharide (LPS) reduced multiple miR-132 targets, suppressed AChE-S more than AChE-R and elevated inflammatory hallmarks...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28209032/surface-plasmon-resonance-investigations-of-bioselective-element-based-on-the-recombinant-protein-a-for-immunoglobulin-detection
#6
A Bakhmachuk, O Gorbatiuk, A Rachkov, B Dons'koi, R Khristosenko, I Ushenin, V Peshkova, A Soldatkin
The developed surface plasmon resonance (SPR) biosensor based on the recombinant Staphylococcal protein A with an additional cysteine residue (SPA-Cys) used as a biorecognition component showed a good selectivity and sensitivity for the immunoglobulin detection. The developed biosensor with SPA-Cys-based bioselective element can also be used as a first step of immunosensor creation. The successful immobilization of SPA-Cys on the nanolayer gold sensor surface of the SPR spectrometer was performed. The efficiency of blocking nonspecific sorption sites on the sensor surface with milk proteins, gelatin, BSA, and HSA was studied, and a rather high efficiency of using gelatin was confirmed...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28208290/biochip-spray-simplified-coupling-of-surface-plasmon-resonance-biosensing-and-mass-spectrometry
#7
Sweccha Joshi, Han Zuilhof, Teris A van Beek, Michel W F Nielen
A simplified coupling of surface plasmon resonance (SPR) immuno-biosensing with ambient ionization mass spectrometry (MS) was developed. It combines two orthogonal analysis techniques: the biosensing capability of SPR and the chemical identification power of high resolution MS. As a proof-of-principle, deoxynivalenol (DON), an important mycotoxin, was captured using an SPR gold chip containing an antifouling layer and monoclonal antibodies against the toxin and, after washing, the chip could be taken out and analyzed by direct spray MS of the biosensor chip to confirm the identity of DON...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28208255/effect-of-probe-probe-distance-on-the-stability-of-dna-hybrids-on-surfaces
#8
Lucyano J A Macedo, Erin N Miller, Aric Opdahl
We have used temperature gradient surface plasmon resonance (SPR) measurements to quantitatively evaluate how the stability of different types of hybrids formed with DNA probes on surfaces is affected by probe spacing. SPR sensors with different average surface densities of probes were prepared by coadsorbing probes with lateral spacers strands comprised of phosphorothioated adenine nucleotides (A15*). Increasing the fraction of A15* spacers in the immobilization solution results in larger distances between probes on the sensor, determined here using a combination of SPR and X-ray photoelectron spectroscopy (XPS) measurements...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28207776/the-improved-efficacy-of-sifuvirtide-compared-with-enfuvirtide-might-be-related-to-its-selectivity-for-the-rigid-biomembrane-as-determined-through-surface-plasmon-resonance
#9
Ping Cao, Guifang Dou, Yuanguo Cheng, Jinjing Che
Most mechanistic studies on human immunodeficiency virus (HIV) peptide fusion inhibitors have focused on the interactions between fusion inhibitors and viral envelope proteins. However, the interactions of fusion inhibitors with viral membranes are also essential for the efficacy of these drugs. Here, we utilized surface plasmon resonance (SPR) technology to study the interactions between the HIV fusion inhibitor peptides sifuvirtide and enfuvirtide and biomembrane models. Sifuvirtide presented selectivity toward biomembrane models composed of saturated dipalmitoylphosphatidylcholine (DPPC) (32-fold higher compared with unsaturated 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine [POPC]) and sphingomyelin (SM) (31-fold higher compared with POPC), which are rigid compositions enriched in the HIV viral membrane...
2017: PloS One
https://www.readbyqxmd.com/read/28199108/application-of-off-rate-screening-in-the-identification-of-novel-pan-isoform-inhibitors-of-pyruvate-dehydrogenase-kinase
#10
Paul A Brough, Lisa Baker, Simon Bedford, Kirsten Brown, Seema Chavda, Victoria Chell, Jalanie D'Alessandro, Nicholas G M Davies, Ben Davis, Loic Le Strat, Alba T Macias, Daniel Maddox, Patrick C Mahon, Andrew J Massey, Natalia Matassova, Sean McKenna, Jonathan D Moore, James B Murray, Christopher J Northfield, Charles Parry, Rachel Parsons, Stephen D Roughley, Terry Shaw, Heather Simmonite, Stephen Stokes, Allan Surgenor, Emma Stefaniak, Alan Robertson, Yikang Wang, Paul Webb, Neil Whitehead, Mike Wood
Libraries of nonpurified resorcinol amide derivatives were screened by surface plasmon resonance (SPR) to determine the binding dissociation constant (off-rate, kd) for compounds binding to the pyruvate dehydrogenase kinase (PDHK) enzyme. Parallel off-rate measurements against HSP90 and application of structure-based drug design enabled rapid hit to lead progression in a program to identify pan-isoform ATP-competitive inhibitors of PDHK. Lead optimization identified selective sub-100-nM inhibitors of the enzyme which significantly reduced phosphorylation of the E1α subunit in the PC3 cancer cell line in vitro...
February 15, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28193510/in-situ-growth-of-au-and-%C3%AE-bi2o3-nanoparticles-on-flower-like-bi2o2co3-a-multi-heterojunction-photocatalyst-with-enhanced-visible-light-responsive-photocatalytic-activity
#11
Peifang Wang, Liya Xu, Yanhui Ao, Chao Wang
In this work, flower-like Au/Bi2O2CO3/Bi2O3 multi-heterojunction photocatalysts were prepared by a in-situ growth method. The as-prepared samples were characterized by different techniques including Transmission Electron Microscopy (TEM), X-ray diffraction (XRD), UV-vis diffuse reflectance spectrometry (DRS), X-ray photoelectron spectroscopy (XPS), Photoluminescence (PL) and photo-induced current. The photocatalytic performance of the as prepared samples was evaluated by degradation of rhodamine B (RhB) under visible light (λ>400nm)...
February 7, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28192972/surface-plasmon-resonance-for-measuring-exocytosis-from-populations-of-pc12-cells-the-mechanisms-of-signal-formation-and-assessment-of-analytical-capabilities
#12
Beatriz Moreira, Jani Tuoriniemi, Naghmeh Kouchak Pour, Lýdia Mihalčíková, Gulnara Safina
Due to cell to cell variation it is difficult to obtain statistically significant data on the frequency of exocytosis events (Rexocytosis, t(-1)m(-2)) with traditional single cell electrophysiological or fluorescence microscopy based methods. Here we take the first steps towards a rapid cost effective surface plasmon resonance (SPR) based method for measuring the Rexocytosis for populations of PC12 cells. The conditions for culturing confluent monolayers on the sensor slides were optimized, and neurotransmitter exocytosis was evoked by injecting solutions with elevated [K(+)]...
February 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28187952/immunogenicity-and-safety-of-an-as03-adjuvanted-h7n1-vaccine-in-healthy-adults-a-phase-i-ii-observer-blind-randomized-controlled-trial
#13
Anuradha Madan, Murdo Ferguson, Eric Sheldon, Nathan Segall, Laurence Chu, Azhar Toma, Paul Rheault, Damien Friel, Jyoti Soni, Ping Li, Bruce L Innis, Anne Schuind
BACKGROUND: H7 influenza strains have pandemic potential. AS03-adjuvanted H7N1 A/mallard/Netherlands/12/2000 split-virion vaccine formulations were evaluated as model H7-subtype vaccine and tested after H7N9 emerged in China, and caused severe human disease with high mortality. METHODS: In this phase I/II, observer-blind, randomized trial in US and Canada, 420 healthy adults (21-64years) were randomized to receive 1 of 4 H7N1 vaccine formulations (3.75 or 7.5μg hemagglutinin adjuvanted with either AS03A or AS03B), 15μg unadjuvanted H7N1 hemagglutinin, or saline placebo, given as 2-dose series...
February 7, 2017: Vaccine
https://www.readbyqxmd.com/read/28187403/green-synthesis-of-silver-nanoparticle-for-the-selective-and-sensitive-colorimetric-detection-of-mercury-ii-ion
#14
Vijay Kumar, Devendra K Singh, Sweta Mohan, Daraksha Bano, Ravi Kumar Gundampati, Syed Hadi Hasan
An ecofriendly and zero cost approach has been developed for the photoinduced synthesis of more stable AgNPs using an aqueous extract of Murraya koenigii (AEM) as a reducing and stabilizing agent. The exposed reaction mixture of AEM and AgNO3 to sunlight turned dark brown which primarily confirmed the biosynthesis of AgNPs. The biosynthesis was monitored by UV-vis spectroscopy which exhibited a sharp SPR band at 430nm after 30min of sunlight exposure. The optimum conditions for biosynthesis of AgNPs were 30min of sunlight exposure, 2...
January 31, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28185720/discovery-of-phenyl-acetamides-as-potent-and-selective-gpr119-agonists
#15
Cheng Zhu, Liping Wang, Yuping Zhu, Zack Zhiqiang Guo, Ping Liu, Zhiyong Hu, Jason W Szewczyk, Ling Kang, Gary Chicchi, Anka Ehrhardt, Andrea Woods, Toru Seo, Morgan Woods, Margaret van Heek, Karen H Dingley, Jianmei Pang, Gino M Salituro, Joyce Powell, Jenna L Terebetski, Viktor Hornak, Louis-Charles Campeau, Robert K Orr, Feroze Ujjainwalla, Michael Miller, Andrew Stamford, Harold B Wood, Timothy Kowalski, Ravi P Nargund, Scott D Edmondson
The paper describes the SAR/SPR studies that led to the discovery of phenoxy cyclopropyl phenyl acetamide derivatives as potent and selective GPR119 agonists. Based on a cis cyclopropane scaffold discovered previously, phenyl acetamides such as compound 17 were found to have excellent GPR119 potency and improved physicochemical properties. Pharmacokinetic data of compound 17 in rat, dog and rhesus will be described. Compound 17 was suitable for QD dosing based on its predicted human half-life, and its projected human dose was much lower than that of the recently reported structurally-related benzyloxy compound 2...
February 1, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28183651/plastic-antibody-based-surface-plasmon-resonance-nanosensors-for-selective-atrazine-detection
#16
Erkut Yılmaz, Erdoğan Özgür, Nilay Bereli, Deniz Türkmen, Adil Denizli
This study reports a surface plasmon resonance (SPR) based affinity sensor system with the use of molecular imprinted nanoparticles (plastic antibodies) to enhance the pesticide detection. Molecular imprinting based affinity sensor is prepared by the attachment of atrazine (chosen as model pesticide) imprinted nanoparticles onto the gold surface of SPR chip. Recognition element of the affinity sensor is polymerizable form of aspartic acid. The imprinted nanoparticles were characterized via FTIR and zeta-sizer measurements...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28176799/tunability-and-sensing-properties-of-plasmonic-1d-photonic-crystal
#17
Mohamed Shaban, Ashour M Ahmed, Ehab Abdel-Rahman, Hany Hamdy
Gold/one-dimensional photonic crystal (Au/1D-PC) is fabricated and applied for sensitive sensing of glucose and different chemical molecules of various refractive indices. The Au layer thickness is optimized to produce surface plasmon resonance (SPR) at the right edge of the photonic band gap (PBG). As the Au deposition time increased to 60 sec, the PBG width is increased from 46 to 86 nm in correlation with the behavior of the SPR. The selectivity of the optimized Au/1D-PC sensor is tested upon the increase of the environmental refractive index of the detected molecules...
February 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28170421/comprehensive-mapping-of-antigen-specific-t-cell-responses-in-hepatitis-c-virus-infected-patients-with-or-without-spontaneous-viral-clearance
#18
Chao Zhang, Rui Hua, Yuanyuan Cui, Shasha Wang, Hongqing Yan, Dongmei Li, Yonghong Zhang, Zhengkun Tu, Pei Hao, Xinyue Chen, Jin Zhong, Junqi Niu, Xia Jin
Elucidating protective immunity against HCV is important for the development of a preventative vaccine. We hypothesize that spontaneous resolution of acute HCV infection offers clue to protective immune responses, and that DAA therapy affects the quality and quantity of HCV-specific T cell responses. To test these hypotheses, we performed T cell epitope mapping in 111 HCV-infected individuals including 61 chronically HCV-1b (CHC-1b) infected, 24 chronically HCV-2a (CHC-2a) infected and 26 spontaneously recovered (SPR) patients with 376 overlapping peptides covering the entire HCV polyprotein...
2017: PloS One
https://www.readbyqxmd.com/read/28167141/2016-updates-to-us-medical-eligibility-criteria-for-contraceptive-use-and-selected-practice-recommendations-for-contraceptive-use-highlights-for-adolescent-patients
#19
REVIEW
Andrea J Hoopes, Katharine B Simmons, Emily M Godfrey, Gina Sucato
The US Medical Eligibility Criteria for Contraceptive Use (US MEC) and US Selected Practice Recommendations for Contraceptive Use (US SPR) provide evidence-based guidance to safely provide contraception counseling and services. Both documents were updated in 2016 and are endorsed by the North American Society for Pediatric and Adolescent Gynecology. The purpose of this mini-review is to highlight updates to the US MEC and US SPR that are most relevant to health care providers of adolescents in order to support dissemination and implementation of these evidence-based best practices...
February 3, 2017: Journal of Pediatric and Adolescent Gynecology
https://www.readbyqxmd.com/read/28165000/high-affinity-anchoring-of-the-decoration-protein-pb10-onto-the-bacteriophage-t5-capsid
#20
Emeline Vernhes, Madalena Renouard, Bernard Gilquin, Philippe Cuniasse, Dominique Durand, Patrick England, Sylviane Hoos, Alexis Huet, James F Conway, Anatoly Glukhov, Vladimir Ksenzenko, Eric Jacquet, Naïma Nhiri, Sophie Zinn-Justin, Pascale Boulanger
Bacteriophage capsids constitute icosahedral shells of exceptional stability that protect the viral genome. Many capsids display on their surface decoration proteins whose structure and function remain largely unknown. The decoration protein pb10 of phage T5 binds at the centre of the 120 hexamers formed by the major capsid protein. Here we determined the 3D structure of pb10 and investigated its capsid-binding properties using NMR, SAXS, cryoEM and SPR. Pb10 consists of an α-helical capsid-binding domain and an Ig-like domain exposed to the solvent...
February 6, 2017: Scientific Reports
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