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Parvin Taghipour, Mostafa Zakariazadeh, Maryam Sharifi, Jafar Ezzati Nazhad Dolatabadi, Abolfazl Barzegar
Bovine serum albumin (BSA) is the most abundant protein in the blood circulation and it is commonly used for drug delivery in blood. Therefore, we aim to study BSA interaction with erlotinib as an anticancer drug using surface plasmon resonance (SPR) and molecular modeling methods under physiological conditions (pH = 7.4). BSA immobilized on carboxymethyl dextran hydrogel Au chip (CMD) after activation with N-hydroxysuccinimide and N-ethyl-N-(3-diethylaminopropyl) carbodiimide and then the erlotinib binding to BSA at different concentrations was evaluated...
April 9, 2018: Journal of Photochemistry and Photobiology. B, Biology
Wei-Feng Peng, Fan Fan, Xin Li, Qian-Qian Zhang, Jing Ding, Xin Wang
PURPOSE: Comorbid depression is common in patients with epilepsy. However, the epilepsy-associated depression is generally atypical and has not been fully recognized by neurologists. This study aimed to compare the behavioral and pathological changes between the chronic lithium chloride-pilocarpine rat epilepsy model (Licl-pilocarpine model) and the Chronic Unpredictable Mild Stress rat depression model (CUMS model), to evaluate for differences between epilepsy-associated depression and primary depression...
April 17, 2018: Epilepsy & Behavior: E&B
Huijie Qi, Lihong Niu, Jie Zhang, Jian Chen, Shujie Wang, Jingjing Yang, Siyi Guo, Tom Lawson, Bingyang Shi, Chunpeng Song
Surface plasmon resonance (SPR) nanosensors based on metallic nanohole arrays have been widely reported to detect binding interactions in biological specimens. A simple and effective method for constructing nanoscale arrays is essential for the development of SPR nanosensors. In this work, we report a one-step method to fabricate nanohole arrays by thermal nanoimprinting in the matrix of IPS (Intermediate Polymer Stamp). No additional etching process or supporting substrate is required. The preparation process is simple, time-saving and compatible for roll-to-roll process, potentially allowing mass production...
April 1, 2018: Science China. Life Sciences
Xuejun Zhang, Ze Wu, Fu Liu, Qiangqiang Fu, Xiaoyong Chen, Jian Xu, Zhaochuan Zhang, Yunyun Huang, Yong Tang, Tuan Guo, Jacques Albert
We propose and demonstrate hydrogen peroxide (H2 O2 ) and glucose concentration measurements using a plasmonic optical fiber sensor. The sensor utilizes a tilted fiber Bragg grating (TFBG) written in standard single mode communication fiber. The fiber is over coated with an nm-scale film of silver that supports surface plasmon resonances (SPRs). Such a tilted grating SPR structure provides a high density of narrow spectral resonances (Q-factor about 105 ) that overlap with the broader absorption band of the surface plasmon waves in the silver film, thereby providing an accurate tool to measure small shifts of the plasmon resonance frequencies...
April 1, 2018: Biomedical Optics Express
Saskia Roesch, Thomas Lindner, Max Sauter, Anastasia Loktev, Paul Flechsig, Martin Müller, Walter Mier, Rolf Warta, Gerhard Dyckhoff, Christel Herold-Mende, Uwe Haberkorn, Annette Altmann
Alpha v beta 6 (αvβ6) integrin is overexpressed by several carcinomas, and thus considered as target for diagnostic imaging and anti-cancer therapies. Recently, we presented SFITGv6 as a novel potential tracer for imaging and targeted therapy of αvβ6 integin-positive carcinomas. Here, we analyzed affinity and specificity of five native αvβ6 integrin-specific binders in comparison to SFITGv6. Methods: Sunflower trypsin inhibitor (SFTI)-1-based peptides containing arginine-glycine-aspartic acid (RGD) motif-spanning octamers of fibronectin (SFFN1), tenascin C (SFTNC), vitronectin (SFVTN), and latency-associated peptides (LAP) 1 (SFLAP1), and 3 (SFLAP3) were synthesized and their binding potential to αvβ6 integrin-expressing head and neck squamous cell carcinoma (HNSCC) cell lines was evaluated...
April 19, 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
Narges Elahi, Mehdi Kamali, Mohammad Hadi Baghersad
Recent advances in nanotechnology are as a result of the development of engineered nanoparticles. Efficiently, metallic nanoparticles have been widely exploited for biomedical application and among them, gold nanoparticles (AuNPs) are highly remarkable. Consequent upon their significant nature, spherical and gold nanorods (Au NRs) nanoparticles attract extreme attention. Their intrinsic features such as optical, electronic, physicochemical and, surface plasmon resonance (SPR); which can be altered by changing the characterizations of particles such as shape, size, aspect ratio, or environment; ease of synthesis and functionalization properties have resulted to various applications in different fields of biomedicine such as sensing, targeted drug delivery, imaging, photothermal and photodynamic therapy as well as the modulation of two or three applications...
July 1, 2018: Talanta
Jiying Xu, Yi Chen
The signal amplification methods of surface plasmon resonance (SPR) are mainly with sample selectivity. Herein proposed is a liquid core coupling SPR sensing method with adjustable sensitivity and universal applicability. A liquid core coupling unit and based on which a wavelength interrogation SPR sensor have been designed and constructed, in which a conventional chip could be used with an ingenious design and the sensitivity can be adjusted by changing the liquid core refractive index. The experimental data revealed that the sensitivity can be enhanced for 7...
July 1, 2018: Talanta
Lasse Thomsen, Leonid Gurevich
The incretin hormone glucagon-like peptide-1 (GLP-1) has been subject to substantial pharmaceutical research regarding the treatment of type 2 diabetes mellitus. However, quantification of GLP-1 levels remains complicated due to the low circulation concentration and concurrent existence of numerous metabolites, homologous peptides, and potentially introduced GLP-1 receptor agonists. Surface plasmon resonance (SPR) facilitates real-time monitoring allowing a more detailed characterisation of the interaction compared with conventional enzyme-linked immunosorbent assays (ELISA)...
April 19, 2018: Journal of Molecular Recognition: JMR
Xiaobing Deng, Xiaoling Zhang, Bo Tang, Hongbo Liu, Qi Shen, Ying Liu, Luhua Lai
Tumor necrosis factor-α (TNF-α) plays a pivotal role in inflammatory response. Dysregulation of TNF can lead to a variety of disastrous pathological effects, including auto-inflammatory diseases. Antibodies that directly targeting TNF-α have been proven effective in suppressing symptoms of these disorders. Compared to protein drugs, small molecule drugs are normally orally available and less expensive. Till now, peptide and small molecule TNF-α inhibitors are still in the early stage of development, and much more efforts should be made...
2018: Frontiers in Chemistry
Yamin Yang, Yue Hu, Henry Du, Lei Ren, Hongjun Wang
Introduction: In recognition of the potentials of gold nanoparticles (Au NPs) in enhanced photodynamic therapy (PDT) for cancer, it is desirable to further understand the shape-dependent surface plasmonic resonance (SPR) properties of various gold nanostructures and evaluate their performances in PDT. Materials and methods: Monodispersed colloidal spherical solid Au NPs were synthesized by UV-assisted reduction using chloroauric acid and sodium citrate, and hollow gold nanorings (Au NRs) with similar outer diameter were synthesized based on sacrificial galvanic replacement method...
2018: International Journal of Nanomedicine
Huan Xiong, Jie Han, Jun Wang, Wenchao Lu, Chen Wang, Yu Chen, Fulin Lian, Naixia Zhang, Yu-Chih Liu, Chenhua Zhang, Hong Ding, Hualiang Jiang, Wencong Lu, Cheng Luo, Bing Zhou
The general control nonrepressed protein 5 (GCN5) plays a crucial role in many biological processes. Dysregulation of GCN5 has been closely related to various human diseases, especially cancers. Hence, the exploitation of small molecules targeting GCN5 is essential for drug design and academic research. Based on the amplified luminescent proximity homogeneous assay screen methodology, we performed high throughput screening and discovered a novel GCN5 inhibitor DC_G16 with 1,8-acridinedione scaffold. Structure optimization led to the identification of a highly potent inhibitor, namely DC_G16-11 with the half-maximal inhibitory concentration (IC50 ) value of 6...
February 14, 2018: European Journal of Medicinal Chemistry
Yi Yang, Xiaoliang Zhao, Jia Li, Hao Jiang, Xindi Shan, Ya Wang, Wenbang Ma, Jiejie Hao, Guangli Yu
We studied the mechanisms underlying the immunostimulatory effects of aβ-1,3/1,6-glucan (BG136) from Durvillaea Antarctica. Our data showed that BG136 promoted the activation of MAPKs and NF-κB signaling pathways and cytokines production. BG136 did not increase MCP-1 or NO production or phosphorylation of NF-κB and MAPK in TLR4 siRNA knockdown cells, indicating that BG136 activates macrophages through TLR4. Flow cytometry analysis and confocal experiment showed that BG136 bound to TLR4 expressed on RAW264...
July 1, 2018: Carbohydrate Polymers
Hui-Juan Li, Peng-Yun Li, Li-Ying Li, Abdul Haleem, Wei-Dong He
Narrowly distributed poly(l-lysine- b - N -isopropylacrylamide) (PLL- b -PNIPAM) was prepared through ring-opening polymerization of ε-benzyloxycarbonyl-l-lysine N -carboxy-α-amino anhydride and atom transfer radical polymerization of NIPAM, followed with the removal of ε-benzyloxycarbonyl group. Then gold nanoparticles (AuNPs) grafted with PLL- b -PNIPAM (PNIPAM-PLL-AuNPs) were obtained by the reduction of chloroauric acid with sodium citrate in the presence of PLL- b -PNIPAM...
April 16, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Hans De Wolf, Laure Cougnaud, Kirsten Van Hoorde, An De Bondt, Joerg K Wegner, Hugo Ceulemans, Hinrich Göhlmann
By adding biological information, beyond the chemical properties and desired effect of a compound, uncharted compound areas and connections can be explored. In this study, we add transcriptional information for 31K compounds of Janssen's primary screening deck, using the HT L1000 platform and assess (a) the transcriptional connection score for generating compound similarities, (b) machine learning algorithms for generating target activity predictions, and (c) the scaffold hopping potential of the resulting hits...
April 2018: Assay and Drug Development Technologies
Tatsuya Higaki, Meng Zhou, Kelly Lambright, Kristin Kirschbaum, Matthew Y Sfeir, Rongchao Jin
The optical properties of metal nanoparticles have attracted wide interest. Recent progress in controlling nanoparticles with atomic precision (often called nanoclusters) provide new opportunities for investigating many fundamental questions, such as the transition from excitonic to plasmonic state--which is a central question in metal nanoparticle research because it provides insights into the origin of surface plasmon resonance (SPR) as well as the formation of metallic bond. However, this question still remains elusive because of the extreme difficulty in preparing atomically precise nanoparticles larger than 2 nm...
April 16, 2018: Journal of the American Chemical Society
Sierra Bowden, Christine Joseph, Shengzhuang Tang, Jayme Cannon, Emily Francis, Michelle Zhou, James R Baker, Seok Ki Choi
Despite its potent antibacterial activities against drug-resistant Gram(+) pathogens, oritavancin remains partially understood in its primary mode of hydrogen bond interaction with a cell-wall peptide regarding the role of its lipophilic 4'-chlorobiphenyl moiety. Here we report a surface plasmon resonance (SPR) study performed in two cell-wall model surfaces, each prepared by immobilization with a vancomycin-susceptible Lys-D-Ala-D-Ala or vancomycin-resistant Lys-D-Ala-D-Lac peptide. Analysis of binding kinetics performed in the peptide surface showed that oritavancin bound ~100 to 1000-fold more tightly than vancomycin in each model surface...
April 13, 2018: Biochemistry
Kostas Perisinakis, Ioannis Seimenis, Antonis Tzedakis, Apostolos Karantanas, John Damilakis
OBJECTIVES: To estimate (a) organ doses and organ-specific radiation-induced cancer risk from a single low-dose CT (LDCT) for lung cancer screening (LCS) and (b) the theoretical cumulative risk of radiation-induced cancer for a typical cohort to be subjected to repeated annual LCS LDCT. METHODS: Sex- and body size-specific organ dose data from scan projection radiography (SPR) and helical CT exposures involved in LCS 256-slice LDCT were determined using Monte Carlo methods...
April 12, 2018: European Radiology
Yu-Wen Su, Wei Wang
Surface plasmon resonance (SPR) has become a well-recognized label-free technique for measuring the binding kinetics between biomolecules since the invention of the first SPR-based immunosensor in 1980s. The most popular and traditional format for SPR analysis is to monitor the real-time optical signals when a solution containing ligand molecules is flowing over a sensor substrate functionalized with purified receptor molecules. In recent years, rapid development of several kinds of SPR imaging techniques have allowed for mapping the dynamic distribution of local mass density within single living cells with high spatial and temporal resolutions and reliable sensitivity...
April 12, 2018: Analytical and Bioanalytical Chemistry
Shang-Wei Li, Chun Ming How, Vivian Hsiu-Chuan Liao
The plasticizer di(2-ethylhexyl) phthalate (DEHP) is an emerging organic contaminant that has represented a risk for organisms present in the environment. However, there is still limited information regarding DEHP-induced multigenerational toxicity and the underlying mechanisms. In this study we investigated the multigenerational toxic effects including locomotive behaviors and reproduction upon prolonged DEHP exposure (from larval L1 to adult) and the underlying mechanisms in the nematode Caenorhabditis elegans...
April 5, 2018: Science of the Total Environment
Roberta de Paula Martins, Viviane Glaser, Aderbal S Aguiar, Priscila Maximiliano de Paula Ferreira, Karina Ghisoni, Débora da Luz Scheffer, Laurence Lanfumey, Rita Raisman-Vozari, Olga Corti, Ana Lucia De Paul, Rodrigo Augusto da Silva, Alexandra Latini
Parkinson's disease (PD), the second-most prevalent neurodegenerative disease, is primarily characterized by neurodegeneration in the substantia nigra pars compacta, resulting in motor impairment. Loss-of-function mutations in parkin are the major cause of the early-onset familial form of the disease. Although rodents deficient in parkin (parkin(-/-)) have some dopaminergic system dysfunction associated with central oxidative stress and energy metabolism deficiencies, these animals only display nigrostriatal pathway degeneration under inflammatory conditions...
April 6, 2018: Cell Biology International
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