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Hae Ja Shin, Woon Ki Lim
In efforts to speed up the assessment of microorganisms, researchers have sought to use bacteriophages as a biosensing tool, due to their host-specificity, wide abundance, and safety. However, the lytic cycle of the phage has limited its efficacy as a biosensor. Here, we cloned a fragment of tail protein J from phage lambda and characterized its binding with the host, E. coli K-12, and other microorganism. The N-terminus of J was fused with a His-tag (6HN-J), overexpressed, purified, and characterized using anti-His monoclonal antibodies...
June 22, 2018: Preparative Biochemistry & Biotechnology
Enrico Tenaglia, Carlotta Guiducci
Capture-SELEX is an effective molecular strategy enabling the discovery of structure-switching aptamers, which might find useful application in molecular detection or separation. We here provide a protocol to perform capture-SELEX for DNA aptamers binding soluble small molecules, which includes a straightforward functional validation by SPR. The SELEX strategy here described is adaptable to any water-soluble molecular target and might foster the development of DNA aptamers binding therapeutic small molecules, at the great advantage of clinical bioanalytics...
2018: Methods in Molecular Biology
Keying Xu, Junwei Shi, Ali Pourmand, Thirupandiyur S Udayakumar, Nesrin Dogan, Weizhao Zhao, Alan Pollack, Yidong Yang
Gold nanoparticles (GNP) have been intensively investigated for applications in cancer imaging and therapy. Most imaging studies focused on microscopic imaging. Their potential as optical imaging probes for whole body small animal imaging has rarely been explored. Taking advantage of their surface plasmon resonance (SPR) properties, we aim to develop a noninvasive diffuse optical imaging method to map the distribution of a special type of GNP, gold nanorods (GNR), in small animals. We developed an integrated dual-modality imaging system capable of both x-ray computed tomography (XCT) and diffuse optical tomography (DOT)...
June 19, 2018: Scientific Reports
Fei Fang, Xiao-Jun Huang, Yi Zong Guo, Xiao Hong, Hui Min Wu, Rong Liu, Da Jing Chen
Cyclodextrins (CDs) are a family of cyclic oligosaccharides and its unique hydrophilic outer surface and lipophilic central cavity facilitate the formation of inclusion complexes with various biomolecules, such as cholesterol and phospholipids, via multi-interactions. Low-density lipoprotein (LDL) is the main carrier of cholesterol in bloodstream and is associated with the progression of atherosclerosis. The surface of LDL is composed of a shell of phospholipids monolayer containing most of the free unesterified cholesterol, as well as the single copy of apolipoprotein B-100...
June 20, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Daniel L Rolnik, Fabricio da Silva Costa, Daljit Sahota, Jon Hyett, Andrew McLennan
AIM: To assess the quality of mean uterine artery pulsatility index (UtAPI) measurement in a first trimester pre-eclampsia screening program. METHODS: Consecutive patients attending first trimester screening for fetal chromosomal abnormalities also had combined screening for pre-eclampsia based on the Fetal Medicine Foundation (FMF) algorithm, in a large practice in Sydney, Australia, from May 2014 to February 2017. Distributions of mean UtAPI multiples of the median (MoM) on the logarithmic scale were plotted in relation to the normal median with 95% confidence interval (CI) for each operator and for each month...
June 19, 2018: Ultrasound in Obstetrics & Gynecology
Sunghwan Kim, Younho Lee, Jun Woo Kim, Young-Jin Son, Min Jung Ma, Jee-Hyun Um, Nam Doo Kim, Sang Hyun Min, Dong Il Kim, Brian B Kim
Activation of adiponectin receptors (AdipoRs) by its natural ligand, adiponectin has been known to be involved in modulating critical metabolic processes such as glucose metabolism and fatty acid oxidation as demonstrated by a number of in vitro and in vivo studies over last two decades. These findings suggest that AdipoRs' agonists could be developed into a potential therapeutic agent for metabolic diseases, such as diabetes mellitus, especially for type II diabetes, a long-term metabolic disorder characterized by high blood sugar, insulin resistance, and relative lack of insulin...
2018: PloS One
Yang Liu, Xueqian Chen, Qiang Ma
In this work, a novel surface plasmon resonance (SPR) enhanced electrochemiluminescence (ECL) biosensing model was first designed based on Au NPs@polydopamine (PDA)@CuInZnS QDs nanocomposite. Au NPs were coated with the PDA layer via the electrostatic force. CuInZnS QDs were bound on the surface of Au NPs@PDA nanocomposite. CuInZnS QDs worked as ECL luminophore in the sensing application. PDA shell not only controlled the separation length between Au NPs and QDs to induce SPR enhanced ECL response, but also limited the potential charge transfer and ECL quenching effect...
June 9, 2018: Biosensors & Bioelectronics
Mitra Amoli-Diva, Rasoul Sadighi-Bonabi, Kamyar Pourghazi
New dual light/temperature-responsive nanocarriers were synthesized using bimetallic plasmonic Au-Ag and Ag-Au nanoparticles (NPs) as cores of vehicles which subsequently functionalized with a UCST-based poly acrylamide-co-acrylonitrile using reversible addition-fragmentation chain transfer for spatiotemporally controlled chemo-photothermal synergistic cancer therapy. The bimetallic cores were assigned to sense wavelengths close to the localized SPR of monometallic NP shell to produce heat which not only can increase the surrounding temperature over the UCST of polymer to open the its valves and promote drug diffusion, but also can kill cancerous cells through photothermal effects with increasing in environment temperature nearly 18 °C after about 5 min radiation...
June 14, 2018: Journal of Pharmaceutical Sciences
Sandra Peherstorfer, Hans Henning Brewitz, Ajay Abisheck Paul George, Amelie Wißbrock, Jana Maria Adam, Lutz Schmitt, Diana Imhof
BACKGROUND: Tight regulation of heme homeostasis is a critical mechanism in pathogenic bacteria since heme functions as iron source and prosthetic group, but is also toxic at elevated concentrations. Hemolysin-activating lysine-acyltransferase (HlyC) from Escherichia coli is crucial for maturation of hemolysin A, which lyses several mammalian cells including erythrocytes liberating large amounts of heme for bacterial uptake. A possible impact and functional consequences of the released heme on events employing bacterial HlyC have remained unexplored...
June 14, 2018: Biochimica et Biophysica Acta
Chenxu Zhao, Ying Gao, Nan Yu, Tiancheng Li, Yang Zhang, Hong Zhang, Guizhi Lu, Yanming Gao, Xiaohui Guo
Neonatal Fc receptor (FcRn) is down-regulated in Hashimoto's thyroiditis (HT) thyrocytes and mediates IgG endocytosis in thyrocytes. The serum distribution of IgG subclasses (of TgAb and TPOAb) differs between HT patients and normal individuals. We aimed to explore the direction and regulation of FcRn-mediated IgG transport in thyrocyte monolayers and the difference between various IgG subclass transport. IgG was transported by FcRn from the basolateral to apical side in the thyrocyte monolayers grown on Transwell filters and the transport was inhibited by IFN-γ and TNF-α...
June 13, 2018: Molecular and Cellular Endocrinology
Aisha Al-Asfar, Zoya Zaheer, Elham Shafik Aazam
Silver-iron bimetallic nanoparticles (BMNPs) were synthesized by using AgNO3 and Fe(NO3 )3 as an Ag/Fe source in presence of Palm dates fruit. Upon addition of extract to a solution of Ag+ and Fe3+ , a prefect transparent stable dark brown color appears with in a few minuets at room temperature. In order to conform the nature of resulting color, UV-visible spectroscopy, transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDX) techniques were used. The absence of surface plasmon resonance (SPR) peaks in the entire UV-visible region suggests the formation of silvercore -ironshell BMNPs...
June 6, 2018: Journal of Photochemistry and Photobiology. B, Biology
Brian M Ilfeld, Rodney A Gabriel, Engy T Said, Amanda M Monahan, Jacklynn F Sztain, Wendy B Abramson, Bahareh Khatibi, John J Finneran, Pia T Jaeger, Alexandra K Schwartz, Sonya S Ahmed
BACKGROUND AND OBJECTIVES: Percutaneous peripheral nerve stimulation (PNS) is an analgesic modality involving the insertion of a lead through an introducing needle followed by the delivery of electric current. This modality has been reported to treat chronic pain as well as postoperative pain the day following knee surgery. However, it remains unknown if this analgesic technique may be used in ambulatory subjects following foot procedures beginning within the recovery room immediately following surgery, and with only short series of patients reported to date, the only available data are derived from strictly observational studies...
June 13, 2018: Regional Anesthesia and Pain Medicine
Fan Zhang, Yaxin Wang, Yongjun Zhang, Lei Chen, Yang Liu, Jinghai Yang
A trilayer SiO₂/Ag/SiO₂ nanocap array was prepared on a two-dimensional template. When annealed at different temperatures, the curvature of the SiO₂/Ag/SiO₂ nanocap arrays increased, which led to Ag nanocap shrinkage. The stress provided by the curved SiO₂ layer induced the formation of Ag nanotwins. Ag nanotwins assisted the growth of nanoparticles when the neighboring nanotwins changed the local misorientations. Nanocap shrinkage reduced the surface plasmon resonance (SPR) coupling between neighboring nanocaps; concurrently, grain growth decreased the SPR coupling between the particles in each nanocap, which led to a red shift of the localized surface plasmon resonance (LSPR) bands and decreased the surface-enhanced Raman scattering (SERS) signals...
June 14, 2018: Nanomaterials
Jonathan Visentin, Lionel Couzi, Claire Dromer, Martine Neau-Cransac, Gwendaline Guidicelli, Vincent Veniard, Karine Nubret-le Coniat, Pierre Merville, Carmelo Di Primo, Jean-Luc Taupin
Human leukocyte antigen (HLA) donor-specific antibodies are key serum biomarkers for assessing the outcome of transplanted patients. Measuring their active concentration, i.e. the fraction that really interacts with donor HLA, and their affinity could help deciphering their pathogenicity. Surface plasmon resonance (SPR) is recognized as the gold-standard for measuring binding kinetics but also active concentrations, without calibration curves. SPR-based biosensors often suffer from non-specific binding (NSB) occurring with the sensor chip surface and the immobilized targets, especially for complex media such as human serum...
June 7, 2018: Biosensors & Bioelectronics
Daniele Vergara, Monica Bianco, Rosanna Pagano, Paola Priore, Paola Lunetti, Flora Guerra, Simona Bettini, Sonia Carallo, Alessandra Zizzari, Elena Pitotti, Livia Giotta, Loredana Capobianco, Cecilia Bucci, Ludovico Valli, Michele Maffia, Valentina Arima, Antonio Gaballo
Protein biomarkers are important diagnostic tools for cancer and several other diseases. To be validated in a clinical context, a biomarker should satisfy some requirements including the ability to provide reliable information on a pathological state by measuring its expression levels. In parallel, the development of an approach capable of detecting biomarkers with high sensitivity and specificity would be ideally suited for clinical applications. Here, we performed an immune-based label free assay using Surface Plasmon Resonance (SPR)-based detection of the soluble form of E-cadherin, a cell-cell contact protein that is involved in the maintaining of tissue integrity...
June 11, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
Muyideen K Tijani, Sreenivasulu B Reddy, Christine Langer, James G Beeson, Mats Wahlgren, Roseangela I Nwuba, Kristina E M Persson
Understanding the functional characteristics of naturally acquired antibodies against P. falciparum merozoite antigens is crucial for determining the protective functions of antibodies. Affinity (measured as kd ) of naturally acquired antibodies against two key targets of acquired immunity, EBA175 and PfRh2, was determined using Surface Plasmon Resonance (SPR) in a longitudinal survey in Nigeria. A majority of the participants, 79% and 67%, maintained stable antibody affinities to EBA175 and PfRh2, respectively, over time...
June 13, 2018: Scientific Reports
Pankaj Arora, Eliran Talker, Noa Mazurski, Uriel Levy
We demonstrate numerically and experimentally the enhancement of Surface Plasmon Resonance (SPR) sensing via dispersion engineering of the plasmonic response using plasmonic nanograting. Following their design and optimization, the plasmonic nanograting structures are fabricated using e-beam lithography and lift-off process and integrated into conventional prism based Kretschmann configuration. The presence of absorptive nanograting near the metal film, provides strong field enhancement with localization and allows to control the dispersion relation which was originally dictated by a conventional SPR structure...
June 13, 2018: Scientific Reports
Shaan Bibi Jaffri, Khuram Shahzad Ahmad
Present study has for the first time reported Prunus cerasifera leaf extract mediated zinc oxide nanoparticles in a green and one pot synthetic mode without utilization of any chemical reducing agents. Synthesized nanoparticles were analyzed by ultraviolet-visible (UV-Vis) spectroscopy, X-ray diffraction (XRD), fourier transmission infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). UV-Vis peak was detected at 380 nm due to surface plasmon resonance (SPR). Variety of biomolecules were revealed by FTIR involved in reduction cum stabilization of zinc oxide nanoparticles...
June 13, 2018: Environmental Technology
Petteri Parkkila, Mohamed Elderdfi, Alex Bunker, Tapani Viitala
Supported lipid bilayers (SLBs) have been used extensively as an effective model of biological membranes, in the context of in vitro biophysics research, and the membranes of liposomes, in the context of the development of nanoscale drug delivery devices. Despite numerous surface-sensitive techniques having been applied to their study, the comprehensive optical characterization of SLBs using surface plasmon resonance (SPR) has not been conducted. In this study, Fresnel multilayer analysis is utilized to effectively calculate layer parameters (thickness and refractive indices) with the aid of dual-wavelength and dispersion coefficient analysis, in which the linear change in refractive index as a function of wavelength is assumed...
June 12, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Md Abdul Khalek, Sujan Chakma, Bikash Kumar Paul, Kawsar Ahmed
In this research work a perfectly circular lattice Photonic Crystal Fiber (PCF) based surface Plasmon resonance (SPR) based sensor has been proposed. The investigation process has been successfully carried out using finite element method (FEM) based commercial available software package COMSOL Multiphysics version 4.2. The whole investigation module covers the wider optical spectrum ranging from 0.48 µm to 1.10 µm. Using the wavelength interrogation method the proposed model exposed maximum sensitivity of 9000 nm/RIU(Refractive Index Unit) and using the amplitude interrogation method it obtained maximum sensitivity of 318 RIU-1 ...
August 2018: Data in Brief
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