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https://www.readbyqxmd.com/read/28643528/modulating-release-of-ranibizumab-and-aflibercept-from-thiolated-chitosan-based-hydrogels-for-potential-treatment-of-ocular-neovascularization
#1
Miguel Moreno, Poh Yih Pow, Tan Su Teng Tabitha, Sonali Nirmal, Andreas Larsson, Krishna Radhakrishnan, Jayabalan Nirmal, Soo Tng Quah, Susana Geifman Shochat, Rupesh Agrawal, Subbu Venkatraman
BACKGROUND: This paper describes the synthesis of thiolated chitosan-based hydrogels with varying degrees of crosslinking that has been utilized to modulate release kinetics of two clinically relevant FDA-approved anti-VEGF protein drugs, ranibizumab and aflibercept. These hydrogels have been fabricated into disc shaped structures for potential use as patches on ocular surface. METHODS: Protein conformational changes and aggregation after loading and release was evaluated by circular dichroism (CD), steady-state tryptophan fluorescence spectroscopy, electrophoresis and size-exclusion chromatography (SEC)...
June 23, 2017: Expert Opinion on Drug Delivery
https://www.readbyqxmd.com/read/28642078/experimental-tests-on-commercial-sweet-product-residue-spr-as-a-suitable-feed-for-anaerobic-bioenergy-h2-ch4-production
#2
Andrea C Luongo Malavè, Debora Fino, Carlos E Gómez Camacho, Bernardo Ruggeri
Food stores can find themselves in the position of having to dispose of different types of products, such as snacks, confectionery, prepackaged food, drinks and others, Sweet Product Residue (SPR), which presents a great opportunity to produce energy through Anaerobic Digestion (AD), due to its high sugar, carbohidrate and fat contents. In order to valorise SPR, this paper takes into consideration the all necessary treatments; owing the fact that the refuses are constitute by an organic part and packaging (plastic, paper and their combinations), a pretreatment able to remove the latter is necessary...
June 19, 2017: Waste Management
https://www.readbyqxmd.com/read/28642073/synthesis-of-biogenic-ag-pd-core-shell-nanoparticles-having-anti-cancer-anti-microbial-functions
#3
Wafa I Abdel-Fattah, M M Eid, Sh I Abd El-Moez, E Mohamed, Ghareib W Ali
Biogenic Ag@Pd core-shell nanoparticles were greenly synthesized within two plant extracts aiming at enhanced anticancer/bactericidal functions. These functions were verified for the two Pd@Ag biogenic core-shell nanoparticles (BCSnp) with constant Pd to several Ag contents. BCSnp were synthesized within two extracts of Almond nuts and Black Berry fruits, four samples each, through simple, low cost and echo friendly microwave route. The BCSnp Surface Plasmon Resonance (SPR) was detected via UV/visible spectrophotometer...
June 19, 2017: Life Sciences
https://www.readbyqxmd.com/read/28637278/functional-and-structural-analysis-of-at-specific-minor-groove-binders-that-disrupt-dna-protein-interactions-and-cause-disintegration-of-the-trypanosoma-brucei-kinetoplast
#4
Cinthia R Millan, Francisco J Acosta-Reyes, Laura Lagartera, Godwin U Ebiloma, Leandro Lemgruber, J Jonathan Nué Martínez, Núria Saperas, Christophe Dardonville, Harry P de Koning, J Lourdes Campos
Trypanosoma brucei, the causative agent of sleeping sickness (Human African Trypanosomiasis, HAT), contains a kinetoplast with the mitochondrial DNA (kDNA), comprising of >70% AT base pairs. This has prompted studies of drugs interacting with AT-rich DNA, such as the N-phenylbenzamide bis(2-aminoimidazoline) derivatives 1 [4-((4,5-dihydro-1H-imidazol-2-yl)amino)-N-(4-((4,5-dihydro-1H-imidazol-2-yl)amino)phenyl)benzamide dihydrochloride] and 2 [N-(3-chloro-4-((4,5-dihydro-1H-imidazol-2-yl)amino)phenyl)-4-((4,5-dihydro-1H-imidazol-2-yl)amino)benzamide] as potential drugs for HAT...
June 16, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28629986/identification-of-influenza-polymerase-inhibitors-targeting-polymerase-pb2-cap-binding-domain-through-virtual-screening
#5
Ming Liu, Edwin Chun-Yeung Lo, Guoxin Wang, Hak-Fun Chow, Jacky Chi-Ki Ngo, David Chi-Cheong Wan, Leo Lit-Man Poon, Pang-Chui Shaw
Influenza A virus is the major cause of epidemics and pandemics worldwide. In this study, virtual screening was used to identify compounds interacting with influenza A polymerase PB2 cap-binding domain (CBD). With a database of 21,351 small molecules, 28 candidate compounds were obtained and four of them (225, 225D1, 426 and 426Br) were found to bind directly to PB2 CBD by surface plasmon resonance (SPR). The evaluation of compounds 426Br and 225 indicated that they could bind to PB2 CBD and inhibit influenza virus at low micromolar concentration...
June 16, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28629186/design-and-performance-of-a-portable-and-multichannel-spr-device
#6
Xiao-Ling Zhang, Yan Liu, Ting Fan, Ning Hu, Zhong Yang, Xi Chen, Zhen-Yu Wang, Jun Yang
A portable multichannel surface plasmon resonance (SPR) biosensor device is presented in this study. As an optical biosensor device, the core component of its light path is a semi-cylindrical prism, which is used as the coupling unit for the excitation of the SPR phenomena. Based on this prism, a wedge-shaped incident light beam including a continuous angle range (10°) is chosen to replace the commonly-used parallel light beam in traditional SPR devices, in which the incident angle is adjusted by a sophisticated mechanical system...
June 19, 2017: Sensors
https://www.readbyqxmd.com/read/28626832/fragment-binding-to-%C3%AE-secretase-1-without-catalytic-aspartate-interactions-identified-via-orthogonal-screening-approaches
#7
Frederik J R Rombouts, Richard Alexander, Erna Cleiren, Alex De Groot, Michel Carpentier, Joyce Dijkmans, Katleen Fierens, Stefan Masure, Diederik Moechars, Martina Palomino-Schätzlein, Antonio Pineda-Lucena, Andrés A Trabanco, Daan Van Glabbeek, Ann Vos, Gary Tresadern
An approach to identify β-secretase 1 (BACE1) fragment binders that do not interact with the catalytic aspartate dyad is presented. A ThermoFluor (thermal shift) and a fluorescence resonance energy transfer enzymatic screen on the soluble domain of BACE1, together with a surface plasmon resonance (SPR) screen on the soluble domain of BACE1 and a mutant of one catalytic Asp (D32N), were run in parallel. Fragments that were active in at least two of these assays were further confirmed using one-dimensional NMR (WaterLOGSY) and SPR binding competition studies with peptidic inhibitor OM99-2...
February 28, 2017: ACS Omega
https://www.readbyqxmd.com/read/28622976/differential-binding-of-the-hiv-1-envelope-to-phosphatidylserine-receptors
#8
Linlin Gu, Brian Sims, Alexandre Krendelchtchikov, Edlue Tabengwa, Qiana L Matthews
Prior work has shown that the HIV-1 envelope of the human immunodeficiency virus (HIV) interact directly with T-cell immunoglobulin mucin (TIM) family proteins. Herein, we demonstrate that HIV-1 envelope glycoproteins from varying HIV-1 clades bind differentially to TIM proteins and functionally similar proteins acting as phosphatidylserine (PtdSer) receptors. Using enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) technology, we show that lysate containing HIV-1 envelope and recombinant HIV-1 envelope glycoproteins bind TIM-4 and advanced glycosylation end product-specific receptor (AGER)...
June 13, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28617674/ultrasensitive-highly-selective-and-real-time-detection-of-protein-using-functionalized-cnts-as-mip-platform-for-fospr-based-biosensor
#9
Anisha Pathak, Shama Parveen, Banshi Dhar Gupta
A facile approach is presented for the detection of Bovine serum albumin (BSA), based on fiber optic SPR (FOSPR) cooperated with molecular imprinting (MIP). The probe is fabricated by exploiting the plasmonic property of silver thin film and vinyl functionalised carbon nanotubes based MIP platform. BSA template molecules are imprinted on the MIP layer coated over multi-walled carbon nanotubes (MWCNTs) to ensure high specificity of the probe in the interfering environments. In addition, FOSPR endorses the sensor capability of real time and remote sensing along with very high sensitivity due to the use of nanostructured MIP platform...
June 15, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28614752/highly-selective-and-sensitive-method-for-cu-2-detection-based-on-chiroptical-activity-of-l-cysteine-mediated-au-nanorod-assemblies
#10
Shahryar Abbasi, Hamzeh Khani
Herein, we demonstrated a simple and efficient method to detect Cu(2+) based on amplified optical activity in the chiral nanoassemblies of gold nanorods (Au NRs). L-Cysteine can induce side-by-side or end-to-end assembly of Au NRs with an evident plasmonic circular dichroism (PCD) response due to coupling between surface plasmon resonances (SPR) of Au NRs and the chiral signal of L-Cys. Because of the obvious stronger plasmonic circular dichrosim (CD) response of the side-by-side assembly compared with the end-to-end assemblies, SS assembled Au NRs was selected as a sensitive platform and used for Cu(2+) detection...
May 30, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28614387/global-sensing-of-the-antigenic-structure-of-herpes-simplex-virus-gd-using-high-throughput-array-based-spr-imaging
#11
Tina M Cairns, Noah T Ditto, Huan Lou, Benjamin D Brooks, Doina Atanasiu, Roselyn J Eisenberg, Gary H Cohen
While HSV-2 typically causes genital lesions, HSV-1 is increasingly the cause of genital herpes. In addition, neonatal HSV infections are associated with a high rate of mortality and HSV-2 may increase the risk for HIV or Zika infections, reinforcing the need to develop an effective vaccine. In the GSK Herpevac trial, doubly sero-negative women were vaccinated with a truncated form of gD2 [gD2(284t)], then examined for anti-gD serum titers and clinical manifestations of disease. Surprisingly, few vaccinees were protected against genital HSV-2 but 86% were protected from genital HSV-1...
June 14, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28611079/elevated-camp-improves-signal-to-noise-ratio-in-amphibian-rod-photoreceptors
#12
Luba A Astakhova, Darya A Nikolaeva, Tamara V Fedotkina, Victor I Govardovskii, Michael L Firsov
The absolute sensitivity of vertebrate retinas is set by a background noise, called dark noise, which originates from several different cell types and is generated by different molecular mechanisms. The major share of dark noise is produced by photoreceptors and consists of two components, discrete and continuous. Discrete noise is generated by spontaneous thermal activations of visual pigment. These events are undistinguishable from real single-photon responses (SPRs) and might be considered an equivalent of the signal...
June 13, 2017: Journal of General Physiology
https://www.readbyqxmd.com/read/28609545/new-ruthenium-based-probes-for-selective-g-quadruplex-targeting
#13
Guillaume Piraux, Laure Bar, Michaël Abraham, Thomas Lavergne, Hélène Jamet, Jérôme Dejeu, Lionel Marcélis, Éric Defrancq, Benjamin Elias
Telomeric regions containing G-quadruplexes (G4s) structures play a pivotal role in the development of cancers. The development of specific binders for G4s is thus of great interest in order to get deeper understanding of the role of these structures, and to ultimately develop new anti-cancer drugs candidates. For several years, RuII complexes have been studied as efficient probe for DNA. Their main interest is the tunability of their structures and properties, and the possibility to use light excitation as a tool to probe the environment of these complexes or selectively trigger a reaction with a biological target...
June 13, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28608810/microcontact-imprinted-plasmonic-nanosensors-powerful-tools-in-the-detection-of-salmonella-paratyphi
#14
Işık Perçin, Neslihan Idil, Monireh Bakhshpour, Erkut Yılmaz, Bo Mattiasson, Adil Denizli
Identification of pathogenic microorganisms by traditional methods is slow and cumbersome. Therefore, the focus today is on developing new and quicker analytical methods. In this study, a Surface Plasmon Resonance (SPR) sensor with a microcontact imprinted sensor chip was developed for detecting Salmonella paratyphi. For this purpose, the stamps of the target microorganism were prepared and then, microcontact S. paratyphi-imprinted SPR chips were prepared with the functional monomer N-methacryloyl-L-histidine methyl ester (MAH)...
June 13, 2017: Sensors
https://www.readbyqxmd.com/read/28602280/mechanism-of-surface-plasmon-resonance-sensing-by-indirect-competitive-inhibition-immunoassay-using-au-nanoparticle-labeled-antibody
#15
Dulal C Kabiraz, Kinichi Morita, Kazuhira Sakamoto, Toshikazu Kawaguchi
We investigated the use of a surface plasmon resonance (SPR) biosensor using an antibody (Ab) labeled with Au-nanoparticle (Ab-AuNP conjugate). As clenbuterol is a small molecule, an indirect competitive inhibition immunoassay was used. The SPR immunoassay using Ab-AuNP conjugate had an extremely low limit of the detection (LOD) with a magnitude of 0.05 ppt (0.05pgmL(-1)), which was 40 times lower than that of unlabeled Ab. To identify the key factor in determining the LOD of the indirect competitive inhibition immunoassay, affinity constants of the surface immunoreaction (K1) and of the premixed solution (αK2) were evaluated...
September 1, 2017: Talanta
https://www.readbyqxmd.com/read/28602084/evaluation-of-the-interaction-between-pesticides-and-a-cell-membrane-model-by-surface-plasmon-resonance-spectroscopy-analysis
#16
Hiroshi Moriwaki, Kotaro Yamada, Hiromitsu Nakanishi
A surface plasmon resonance (SPR) spectroscopy analysis was used for the characterization of the interaction between pesticides and a cell membrane model. A liposome was immobilized onto the surface of the SPR sensor chip (L1), and the lipid bilayer membrane formed on the sensor chip was regarded as the cell membrane model. The solution containing a pesticide was flowed onto the sensor chip, and an SPR sensorgram, which reflected the interaction between the pesticide and the lipid bilayer membrane, was obtained...
June 20, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28601649/gum-ghatti-mediated-one-pot-green-synthesis-of-optimized-gold-nanoparticles-investigation-of-process-variables-impact-using-box-behnken-based-statistical-design
#17
Md Sabir Alam, Arun Garg, Faheem Hyder Pottoo, Mohammad Khalid Saifullah, Abu Izneid Tareq, Ovais Manzoor, Mohd Mohsin, Md Noushad Javed
Due to unique inherent catalytic characteristics of different size, shape and surface functionalized Gold nanoparticles, their potential application is being explored in various fields such as drug delivery, biosensor, diagnosis and theranostics. However Conventional process for synthesis of these metallic nanoparticles utilizes toxic reagents as reducing agents, additional capping agent for stability as well as surface functionalization for drug delivery purposes. Hence, in this work suitability of Gum Ghatti for reducing, capping and surface functionalization during the synthesis of stable Gold nanoparticles were duly explored...
June 7, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28600792/an-enhancement-of-antimicrobial-efficacy-of-biogenic-and-ceftriaxone-conjugated-silver-nanoparticles-green-approach
#18
Rajasree Shanmuganathan, Davoodbasha MubarakAli, Desika Prabakar, Harshiny Muthukumar, Nooruddin Thajuddin, Smita S Kumar, Arivalagan Pugazhendhi
Of the various methods explored for the synthesis of nanoparticles, biogenesis of silver nanoparticles (AgNPs) received great attention due to their versatile properties. In this report, Daucus carota extract was used for the synthesis of AgNPs and ceftriaxone was conjugated with AgNPs to enhance their antimicrobial efficacy. The conjugated and unconjugated AgNPs were characterized by adopting UV-Vis spectroscopy, FTIR, AFM, DLS, and TEM, which revealed the SPR peak at 420 nm and spherical shaped nanoparticles of 20 nm size, respectively...
June 9, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28598594/tethering-growth-factors-to-collagen-surfaces-using-copper-free-click-chemistry-surface-characterization-and-in-vitro-biological-response
#19
Hyun Jong Lee, Gabriella Fernandes-Cunha, Ilham Putra, Won-Gun Koh, David Myung
Surface modifications with tethered growth factors have mainly been applied to synthetic polymeric biomaterials in well-controlled, acellular settings, followed by seeding with cells. The known bio-orthogonality of copper-free click chemistry provides an opportunity to not only use it in vitro to create scaffolds or pro-migratory tracks in the presence of living cells, but also potentially apply it to living tissues directly as a coupling modality in situ. In this study, we studied the chemical coupling of growth factors to collagen using biocompatible copper-free click chemistry and its effect on the enhancement of growth factor activity in vitro...
June 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28597893/observing-the-dynamic-hot-spots-on-two-dimensional-au-nanoparticles-monolayer-film
#20
Chenjie Zhang, Enming You, Qi Jin, Yaxian Yuan, Minmin Xu, Songyuan Ding, Jianlin Yao, Zhongqun Tian
Interparticle spacing was controlled by evaporating water on 2D Au nanoparticles arrays. Relationships among SERS effect, SPR catalysis, and gap distance were experimentally and theoretically studied.
June 9, 2017: Chemical Communications: Chem Comm
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