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cavia porcellus

Carolina Schencke, Adriana Vasconcellos, Cristian Sandoval, Paulina Torres, Francisca Acevedo, Mariano Del Sol
BACKGROUND: In the context of the search for cost-efficient treatments, Ulmo (Eurcyphia cordifolia) honey is an excellent alternative for treating burn wounds and could have a profound medical, social, and economic impact. Ascorbic acid is an enzymatic co-factor necessary for the synthesis of collagen and the proliferation of fibroblasts and has been proposed as a coadjuvant to strengthen the healing effects of honey. The aim of this work was to evaluate by morphometric studies the healing wounds caused by burns treated with Ulmo honey alone and supplemented with ascorbic acid in guinea pig (Cavia porcellus)...
2016: Burns and trauma
L Fu, Y L Hou, X Ding, Y J Du, H Q Zhu, N Zhang, W R Hou
The complementary DNA (cDNA) of the giant panda (Ailuropoda melanoleuca) ferritin light polypeptide (FTL) gene was successfully cloned using reverse transcription-polymerase chain reaction technology. We constructed a recombinant expression vector containing FTL cDNA and overexpressed it in Escherichia coli using pET28a plasmids. The expressed protein was then purified by nickel chelate affinity chromatography. The cloned cDNA fragment was 580 bp long and contained an open reading frame of 525 bp. The deduced protein sequence was composed of 175 amino acids and had an estimated molecular weight of 19...
August 30, 2016: Genetics and Molecular Research: GMR
David A Bemis, Brian H Johnson, Mary Jean Bryant, Rebekah D Jones, Brynn V McCleery, Cheryl B Greenacre, Vincent Perreten, Stephen A Kania
An organism reported in the early literature to be a rare cause of cervical lymphadenitis in guinea pigs, Streptobacillus moniliformis, has been reclassified as Caviibacter abscessus We describe a series of sequential cases of abscesses in guinea pigs that were presented to our clinic from which the only agent isolated was a unique, serum-requiring bacterium. Discrete colonies were not detected in 6.5% CO2 or anaerobically on routine primary isolation media containing up to 5% whole sheep blood, with and without cysteine, vitamin K, and hemin supplementation after 7 days of incubation at 37°C...
November 2016: Journal of Veterinary Diagnostic Investigation
Yvette Ellen, Paul Flecknell, Matt Leach
To manage pain effectively in people and animals, it is essential to recognise when pain is present and to assess its intensity. Currently there is very little information regarding the signs of post-surgical pain or its management in guinea pigs. Studies from other rodent species indicate that behaviour-based scoring systems can be used successfully to detect pain and evaluate analgesic efficacy. This preliminary study aimed to establish whether behaviour-based scoring systems could be developed to assess post-surgical pain in guinea pigs...
2016: PloS One
Ana C Lima, Min Jung, Jannette Rusch, Abul Usmani, Alexandra Lopes, Donald F Conrad
Advanced methods of cellular purification are required to apply genome technology to the study of spermatogenesis. One approach, based on flow cytometry of murine testicular cells stained with Hoechst-33342 (Ho-FACS), has been extensively optimized and currently allows the isolation of 9 germ cell types. This staining technique is straightforward to implement, highly effective at purifying specific germ cell types and yields sufficient cell numbers for high throughput studies. Ho-FACS is a technique that does not require species-specific markers, but whose applicability to other species is largely unexplored...
August 24, 2016: Biology of Reproduction
A Balsiger, M Clauss, A Liesegang, B Dobenecker, J-M Hatt
When offered diets with hay ad libitum, rabbits (Oryctolagus cuniculus) clearly prefer open dishes over nipple drinkers, but whether this preference also applies in guinea pigs (Cavia porcellus) is unsure. We tested the drinker preference of 10 guinea pigs when offered open dishes (OD) and nipple drinkers (ND) simultaneously and measured the amount of water consumed by each animal on four different diets (grass hay 100%, or as 10% of intake on diets of fresh parsley, seed mix or pelleted complete feed, respectively) on either of the drinking systems...
July 23, 2016: Journal of Animal Physiology and Animal Nutrition
Seyed Mehdi Rajaei, Maneli Ansari Mood, Reza Sadjadi, Farzaneh Azizi
The purpose of this study was to evaluate intraocular pressure (IOP) by means of rebound tonometry, to assess tear production by using the endodontic absorbent paper point tear test (EAPTT) and phenol red thread test (PRTT), and to determine the effects of time of day on IOP and tear production in guinea pigs. The study population comprised 24 healthy adult guinea pigs (12 male, 12 female; 48 eyes) of different breeds and ranging in age from 12 to 15 mo. IOP and tear production were measured at 3 time points (0700, 1500, and 2300) during a 24-h period...
2016: Journal of the American Association for Laboratory Animal Science: JAALAS
D Rozanska, P Rozanski, M Orzelski, N Chlebicka, K Putowska
AIMS: To describe a simple, minimally invasive method of ovariohysterectomy via a unilateral flank approach in guinea pigs, for use in routine desexing of healthy female guinea pigs or treatment of ovarian cysts. METHODS: The subjects of this retrospective study were 41 client-owned guinea pigs submitted for routine desexing or treatment of ovarian cysts. They included 16 healthy female guinea pigs aged 8-12 months (Group 1), and 15 females aged from 9 months to 3 years (Group 2), and 10 females aged from 3 to 7 years (Group 3) with different-sized ovarian cysts...
November 2016: New Zealand Veterinary Journal
Stephen D White, David Sanchez-Migallon Guzman, Joanne Paul-Murphy, Michelle G Hawkins
BACKGROUND: Guinea pigs (Cavia porcellus) are popular companion animals with reported skin diseases, but most reports are accessed from textbooks or review articles. HYPOTHESIS/OBJECTIVES: To document skin diseases and their prevalence in companion guinea pigs in northern California, USA, and to investigate predilections for the most common conditions over a 25 year period. ANIMALS: Two hundred and ninety three guinea pigs from the hospital population met inclusion criteria...
October 2016: Veterinary Dermatology
Charles P Byrd, Christina Winnicker, Brianna N Gaskill
Domestic guinea pigs (Cavia aperea f. porcellus) in laboratories have been shown to actively avoid the centers of their cages. This experiment tested a novel, dark-colored "shader" placed over the central portion of a cage. Based on the observed behavior of wild guinea pig species, it was hypothesized that utilization of the central portion of the cage would increase when the shader was present. Eleven male and 11 female albino, 3-week-old Hartley guinea pigs (Crl:HA) experienced the control and treatment conditions in a crossover study design...
October 2016: Journal of Applied Animal Welfare Science: JAAWS
Jay P Graham, Karla Vasco, Gabriel Trueba
Domestic animals and animal products are the source of pathogenic Campylobacter jejuni and C. coli in industrialized countries, yet little is known about the transmission of these bacteria in developing countries. Guinea pigs (Cavia porcellus) are commonly raised for food in the Andean region of South America, however, limited research has characterized this rodent as a reservoir of zoonotic enteric pathogens. In this study, we examined the prevalence of Campylobacter spp. in 203 fecal samples from domestic animals of 59 households in a semi-rural parish of Quito, Ecuador...
June 2016: Environmental Microbiology Reports
Gale A Kleven, Prianca Joshi
The Hairless strain of guinea pigs (Cavia porcellus) is the result of a spontaneous recessive mutation first identified at the Institute Armand Frappier (IAF) in 1978. Despite the longstanding availability of this strain, little is known about its thermoregulatory behavior. The aim of this study was to determine temperature preference in Hartley and Hairless guinea pigs by observing each strain in a ring-shaped apparatus containing a nonlinear temperature gradient. Temperatures were maintained by separately controlled heating mats lining the apparatus...
March 2016: Journal of the American Association for Laboratory Animal Science: JAALAS
L C M Barboza, K Lezirovitz, D B Zanatta, B E Strauss, R C Mingroni-Netto, J Oiticica, L A Haddad, R F Bento
In mammals, damage to sensory receptor cells (hair cells) of the inner ear results in permanent sensorineural hearing loss. Here, we investigated whether postnatal mouse inner ear progenitor/stem cells (mIESCs) are viable after transplantation into the basal turns of neomycin-injured guinea pig cochleas. We also examined the effects of mIESC transplantation on auditory functions. Eight adult female Cavia porcellus guinea pigs (250-350 g) were deafened by intratympanic neomycin delivery. After 7 days, the animals were randomly divided in two groups...
2016: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
Liping Feng, Jiawen Zhu, Haitao Chang, Xiaoping Gao, Cheng Gao, Xiaofeng Wei, Fangyan Yuan, Weicheng Bei
The main role of CodY, a global regulatory protein in most low G + C gram-positive bacteria, is in transcriptional repression. To study the functions of CodY in Streptococcus suis serotype 2 (S. suis 2), a mutant codY clone named ∆codY was constructed to explore the phenotypic variation between ∆codY and the wild-type strain. The result showed that the codY mutation significantly inhibited cell growth, adherence and invasion ability of S. suis 2 to HEp-2 cells. The codY mutation led to decreased binding of the pathogen to the host cells, easier clearance by RAW264...
2016: Scientific Reports
Maneli Ansari-Mood, Seyed Mehdi-Rajaei, Reza Sadjadi, Masoud Selk-Ghaffari, David L Williams
The objective of this study was to measure intraocular pressure (IOP) in intact, healthy guinea pigs (15 male, 15 female) every 2 h for a 24-h period. First, IOP was measured by using rebound tonometry (RBT). After a 1-min rest period, 0.5% proparacaine ophthalmic solution, a topical anesthetic, was applied to both eyes; 4 min after anesthetic instillation, IOP was measured by using applanation tonometry (APT). The IOP was lower during the light period (0700 to 1900) than during the dark phase (2000 to 0600)...
January 2016: Journal of the American Association for Laboratory Animal Science: JAALAS
Misha L Dunbar, Emily M David, Marian R Aline, Jennifer L Lofgren
Although guinea pigs (Cavia porcellus) have been used in research for more than a century and remain the most prevalent USDA-covered species, little has been elucidated regarding the recognition of clinical pain or analgesic efficacy in this species. We sought to assess pain in guinea pigs by using newer, clinically relevant methods that have been validated in other rodent species: the behavioral ethogram and cageside proxy indicator. In this study, 10 male guinea pigs underwent electronic von Frey testing of nociception, remote videorecording of behavior, and cageside assessment by using time-to-consumption (TTC) of a preferred treat test...
January 2016: Journal of the American Association for Laboratory Animal Science: JAALAS
Nikolaus von Engelhardt, Gabriele J Kowalski, Anja Guenther
BACKGROUND: Prenatal conditions influence offspring development in many species. In mammals, the effects of social density have traditionally been considered a detrimental form of maternal stress. Now their potential adaptive significance is receiving greater attention.Sex-specific effects of maternal social instability on offspring in guinea pigs (Cavia aperea f. porcellus) have been interpreted as adaptations to high social densities, while the effects of low social density are unknown...
2015: Frontiers in Zoology
Tobias Eisenberg, Stefanie P Glaeser, Christa Ewers, Torsten Semmler, Birgit Drescher, Peter Kämpfer
A pleomorphic, Gram-stain-negative, rod-shaped, indole-, oxidase- and catalase-negative, non-spore-forming, non-motile bacterium was originally isolated from the mandibular lymph node of a guinea pig and deposited as Streptobacillus moniliformis CCUG 39713 in 1998. A second strain, 151011837, was isolated from an identical lesion in a guinea pig in Germany in 2015. On the basis of 16S rRNA gene sequence analyses, these strains displayed highest sequence similarities with Sneathia sanguinegens NTS65407T (93...
April 2016: International Journal of Systematic and Evolutionary Microbiology
Wendy R Williams, Matthew S Johnston, Sarah Higgins, Angelo A Izzo, Lon V Kendall
The guinea pig is a common animal model that is used in biomedical research to study a variety of systems, including hormonal and immunological responses, pulmonary physiology, corticosteroid response and others. However, because guinea pigs are evolutionarily a prey species, they do not readily show behavioral signs of disease, which can make it difficult to detect illness in a laboratory setting. Minimally invasive blood tests, such as complete blood counts and plasma biochemistry assays, are useful in both human and veterinary medicine as an initial diagnostic technique to rule in or rule out systemic illness...
January 2016: Lab Animal
Bethany S Zarndt, Jessica N Buchta, Lindsey S Garver, Silas A Davidson, Edgar D Rowton, Kenneth E Despain
Small mammals have difficulty maintaining body temperature under anesthesia. This hypothermia is a potential detriment not only to the health and comfort of the animal but also to the integrity of any treatment given or data gathered during the anesthetic period. Using an external warming device to assist with temperature regulation can mitigate these effects. In this study, we investigated the ability of an advanced warming device that uses far-infrared (FIR) heating and responds to real-time core temperature monitoring to maintain a normothermic core temperature in guinea pigs...
November 2015: Journal of the American Association for Laboratory Animal Science: JAALAS
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