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manual mycobacteria growth indicator tube

C D Kelly-Cirino, E Musisi, P Byanyima, S Kaswabuli, A Andama, A Sessolo, I Sanyu, J Zawedde, P S Curry, L Huang
OMNIgene·SPUTUM (OM-S) is a sample transport reagent designed to work with all tuberculosis diagnostics while eliminating the need for cold chain. OM-S-treated sputum samples were assayed in several tests after multiday holds. Raw sputa from 100 patients underwent direct smear microscopy, were manually split and assigned to the OM-S group [OM-S added at collection (no other processing required) and tested after 0- to 5-day holds at room temperature] or standard-of-care (SOC) group (NaOH/N-acetyl l-cysteine decontamination, all tested on day of collection)...
June 2017: Journal of Epidemiology and Global Health
Samantha Flores-Treviño, Rayo Morfín-Otero, Eduardo Rodríguez-Noriega, Esteban González-Díaz, Héctor R Pérez-Gómez, Virgilio Bocanegra-García, Lucio Vera-Cabrera, Elvira Garza-González
Determining the genetic diversity of M. tuberculosis strains allows identification of the distinct Mycobacterium tuberculosis genotypes responsible for tuberculosis in different regions. Several studies have reported the genetic diversity of M. tuberculosis strains in Mexico, but little information is available from the state of Jalisco. Therefore, the aim of this study was to determine the genetic diversity of Mycobacterium tuberculosis clinical isolates from Western Mexico. Sixty-eight M. tuberculosis isolates were tested for susceptibility to first-line drugs using manual Mycobacteria Growth Indicator Tube method and genotyped using spoligotyping and IS6110-restriction fragment length polymorphism (RFLP) pattern analyses...
2015: PloS One
N Karabulut, B Bayraktar, Y Bulut
BACKGROUND AND OBJECTIVES: Antimycobacterial susceptibility tests take weeks, and delayed therapy can lead to spread of Mycobacterium tuberculosis. Therefore, rapid, accurate and cost-effective methods are required for proper therapy selection. In this study, the Mycobacteria growth indicator tube (MGIT) and epsilometer test (Etest) methods were compared to the agar proportion method for susceptibility testing of Mycobacterium tuberculosis. MATERIALS AND METHODS: The susceptibility tests against isoniazid (INH), rifampin (RIF), streptomycin (STM) and ethambutol (ETM) of 51 M...
July 2014: Indian Journal of Medical Microbiology
Vicente Ausina Ruiz, Gema Fernández-Rivas, Cristina Vilaplana Messeguer
Evaluation of: Adikaram CP, Perera J, Wijesundera SS. The manual Mycobacteria Growth Indicator Tube and the Nitrate Reductase Assay for the rapid detection of rifampicin resistance of M. tuberculosis in low resource settings. BMC Infect. Dis. 12, 326 (2012). The control of drug-resistant tuberculosis (TB) requires an accurate and a rapid diagnostic for the detection of critical patterns of drug resistance. Rifampicin resistance is a good predictor of the presence of multidrug-resistant TB. Traditionally, in vitro susceptibility methods for Mycobacterium tuberculosis are time consuming and laborious...
April 2013: Expert Review of Molecular Diagnostics
Chamila P Adikaram, Jennifer Perera, Sandya S Wijesundera
BACKGROUND: Tuberculosis (TB) is a disease of poverty that contributes significantly to ill-health in developing countries. Drug resistant TB is a major challenge to disease control. Early diagnosis and rapid determination of drug sensitivity is of paramount importance in eradication of TB. Although automated liquid culture based methods are available for rapid detection of drug resistance, the high cost of these tests prevent them from being used routinely in low resource settings. This study compares two phenotypic methods, the manual Mycobacteria Growth Indicator Tube (MGIT) and the Nitrate Reductase Assay (NRA) in liquid medium, with the agar proportion method (APM), the gold standard for susceptibility testing of Mycobacterium tuberculosis...
2012: BMC Infectious Diseases
Mohd Nor Fadzilah, Kee Peng Ng, Yun Fong Ngeow
A prospective study was conducted on 510 respiratory specimens for the presence of M. tuberculosis detected by direct acid-fast bacilli (AFB) smear examination, culture in the Manual Mycobacteria Growth Indicator Tube (BBL MGIT, Becton-Dickinson) and culture on Lowenstein-Jensen (LJ) medium. From positive BBL MGIT tubes, Ziehl-Neelsen and Gram stains were performed and subcultures were put up on LJ medium. A total of 101 (19.8%) specimens were positive by the BBL MGIT, 60 (11.8%) by primary LJ medium culture, 31 (6...
December 2009: Malaysian Journal of Pathology
E J Shitaye, V Beran, J Svobodová, M Morávková, V Babák, I Pavlík
Different methods for the detection of Mycobacterium avium ssp. avium (MAA) in naturally infected hens were compared. They included the conventional culture method (solid Herrold's and Stonebrink media and liquid Sula medium) and newly developed liquid culture systems, the manual mycobacteria growth indicator tube (M-MGIT) and the fully automated BACTEC MGIT 960 system (A-MGIT). 152 tissues originating from 15 naturally infected hens have been processed. The overall detection rates (percentage of positive cultures from the number of positive cultures determined by all the methods together) were 60, 70 and 76 % for the conventional media, M-MGIT and A-MGIT systems, respectively, the mean time of mycobacteria detection being 32...
2009: Folia Microbiologica
M Muyoyeta, J A Schaap, P De Haas, W Mwanza, M W Muvwimi, P Godfrey-Faussett, H Ayles
SETTING: National TB Reference Laboratory, Zambia. OBJECTIVE: To compare four TB culture systems when used in a resource-limited setting. DESIGN: Comparison of four culture systems: automated Mycobacterium Growth Indicator Tube (AMGIT) 960, manual MGIT (MMGIT) and two Löwenstein-Jensen (LJ) culture media-commercial (CLJ) and homemade (HLJ). RESULTS: A total of 1916 sputum specimens were received, of which 261 (13.6%) were positive on microscopy...
April 2009: International Journal of Tuberculosis and Lung Disease
E Fegou, E Jelastopulu, S Nicolaou, M Sevdali, S Anagnostou, S Kanavaki, G Dimitracopoulos, I Spiliopoulou
BACKGROUND: Clinical microbiology laboratories should provide reliable results on susceptibility testing of Mycobacterium tuberculosis to different agents. METHODS: The manual Mycobacteria Growth Indicator Tube (MGIT) and Etest were compared to the method of proportion (MOP) for susceptibility testing of 88 clinical isolates of M. tuberculosis against isoniazid (INH), rifampin (RIF), streptomycin (STR) and ethambutol (EMB). Isolates were recovered from different patients and were identified at species level by PCR and hybridization...
2006: Chemotherapy
B Kozáková
Diagnosis of tuberculosis is based on clinical symptoms, lung X-ray, skin tuberculin test and primarily on the detection of the causative agent Mycobacterium tuberculosis by direct microscopy of biological specimens and culture on solid egg media and in liquid media (Ogawa, Löwenstein-Jensen, Sula). Slow growth of most mycobacterial species is a limiting factor in both the confirmation of etiology and subsequent drug susceptibility tests and species identification that are of crucial relevance to early institution of treatment, selection of treatment regimen and implementation of antiepidemic measures...
February 2006: Epidemiologie, Mikrobiologie, Imunologie
H Syre, H Valvatne, P Sandven, H M S Grewal
OBJECTIVES: To evaluate the inexpensive colorimetric nitrate reductase-based antibiotic susceptibility (CONRAS) assay for testing the susceptibility of Mycobacterium tuberculosis to streptomycin and ethambutol in liquid cultures, and to compare the CONRAS test with the manual mycobacteria growth indicator tube (MGIT) test, using the radiometric BACTEC 460TB method as reference. METHODS: A total of 89 M. tuberculosis isolates were tested for susceptibility to streptomycin and ethambutol using the CONRAS and manual MGIT methods and the results were compared with BACTEC 460TB...
May 2006: Journal of Antimicrobial Chemotherapy
Sabai Phyu, Thandar Lwin, Ti Ti, Win Maung, Win W Mar, San S Shein, Harleen M S Grewal
The extent of drug resistant tuberculosis (TB) in the capital city of Myanmar, Yangon has not yet been reported. This study aimed to determine the proportion and pattern of drug resistance to first-line anti-TB drugs, among Mycobacterium tuberculosis complex isolates from sputum smear positive TB patients who attended National TB Programme Yangon centres in April-August and October-December 2002. Drug susceptibility was determined by the Mycobacteria Growth Indicator Tube manual system (Becton Dickinson, MD, USA)...
2005: Scandinavian Journal of Infectious Diseases
Kati Adjers-Koskela, Marja-Leena Katila
The objective of the study was to compare the manual Mycobacteria Growth Indicator Tube (MGIT) method and the BACTEC MGIT 960 system to the BACTEC 460 method for susceptibility testing of Mycobacterium tuberculosis. The evaluation was based on testing of 36 M. tuberculosis strains with various susceptibilities to isoniazid (INH), rifampin (RMP), ethambutol (EMB), and streptomycin (SM). In addition, five of the strains generating discrepant results in testing for EMB were analyzed for heteroresistance. For INH, the susceptibility test results obtained by the MGIT 960 and the manual MGIT systems agreed with the BACTEC 460 results in 94 and 97% of the cases, respectively...
March 2003: Journal of Clinical Microbiology
V Goloubeva, M Lecocq, P Lassowsky, F Matthys, F Portaels, I Bastian
The manual Mycobacteria Growth Indicator Tube (MGIT) method was evaluated for performing direct and indirect drug susceptibility testing (DST) of Mycobacterium tuberculosis for isoniazid and rifampin on 101 strongly smear-positive sputum specimens in a Siberian prison hospital. Using the indirect method of proportion (MOP) as the "gold standard," the accuracies of isoniazid and rifampin susceptibility testing by the direct MGIT system were 97.0 and 94.1%, respectively. The accuracy of the indirect MGIT system was 98...
April 2001: Journal of Clinical Microbiology
L Heifets, T Linder, T Sanchez, D Spencer, J Brennan
Two manual liquid medium systems, the Mycobacteria Growth Indicator Tube (MGIT) and MB Redox tube systems, were evaluated in comparison to the radiometric BACTEC-460 semiautomated system for recovery of Mycobacterium tuberculosis from sputum specimens. The highest level of recovery, from a total of 77 culture-positive specimens, occurred with the BACTEC-460 system (92.2%), followed by the MB Redox tube (80.5%) and the MGIT (63.6%) systems. The shortest time to detection was observed also among the cultures in BACTEC-460: a mean of 12 days to a growth index (GI) of 10 and 15 days to a GI of 500...
March 2000: Journal of Clinical Microbiology
C Piersimoni, C Scarparo, P Cichero, M De Pezzo, I Covelli, G Gesu, D Nista, M Scagnelli, F Mandler
MB-Redox is a new manual culture system designed for the recovery of mycobacteria from clinical specimens. It consists of a liquid medium (modified Kirchner medium) containing a redox indicator, a colorless tetrazolium salt, which is reduced to colored formazan by actively growing mycobacteria. Acid fast bacilli (AFB) are easily detected in the medium as pink to purple pinhead-sized particles. We report the results of a multicenter study (involving four Italian microbiology laboratories processing 2370 clinical specimens) aiming to evaluate the recovery rates of AFB and time required for their detection by using the MB-Redox medium...
August 1999: Diagnostic Microbiology and Infectious Disease
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