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Haisheng Liu, Changbei Ma, Meijuan Zhou, Hanchun Chen, Hailun He, Kemin Wang
This work demonstrates a novel method for DNA methyltransferase (MTase) activity detection with a quencher-free molecular beacon (MB) probe based on exonuclease (Exo) III-assisted signal amplification. In the presence of Dam MTase and DpnI endonuclease, the elaborately designed hairpin substrate (MB1) was cleaved into two parts (part A and part B). Exo III can then digest part A and release a single-stranded target of the 2-aminopurine-labeled MB (MB2). Subsequently, the MB2 can hybridize with its target to form a double-stranded structure with a protruding 3'-terminus and then trigger the digestion of MB2 by Exo III...
November 2016: Analytical and Bioanalytical Chemistry
Jonas Koch, Julia Lüdemann, Rieke Spies, Marco Last, Chris T Amemiya, Thorsten Burmester
Myoglobin is a respiratory protein that serves as a model system in a variety of biological fields. Its main function is to deliver and store O2 in the heart and skeletal muscles, but myoglobin is also instrumental in homeostasis of nitric oxide (NO) and detoxification of reactive oxygen species (ROS). Almost every vertebrate harbors a single myoglobin gene; only some cyprinid fishes have two recently duplicated myoglobin genes. Here we show that the West African lungfish Protopterus annectens has at least seven distinct myoglobin genes (PanMb1-7), which diverged early in the evolution of lungfish and showed an enhanced evolutionary rate...
August 10, 2016: Molecular Biology and Evolution
Y Yang, M D Sumption, E W Collings
High temperatures and under pressure (HTP) processing has been used to study the effects of chemical doping in MgB2. ZrB2, TiB2 and NbB2 were selected as additives since, like MgB2, they have an AlB2-type structure and similar lattice parameters. Dy2O3 was selected as it has been reported to generate nanoscale, secondary intragrain phases in MgB2. While C is known to enter the B-sublattice readily, attempts to dope Zr and other elements onto the Mg site have been less successful due to slow bulk diffusion, low solubility in MgB2, or both...
2016: Scientific Reports
Virginie Doyen, Gabrielle Pilcer, Phong Huy Duc Dinh, Francis Corazza, Alfred Bernard, Pierre Bergmann, Nicolas Lefevre, Karim Amighi, Olivier Michel
AIMS: In drug development, the anti-inflammatory properties of new molecules in the lung are currently tested using the inhaled lipopolysaccharide (LPS) model. The total and regional lung bioavailability of inhaled particles depends significantly on their size. The objective of the present study was to compare inflammatory responses in healthy volunteers after the inhalation of LPS of varying droplet size. METHODS: Three nebulizers were characterized by different droplet size distributions [mean mass median aerodynamic diameters: Microcirrus (2...
June 22, 2016: British Journal of Clinical Pharmacology
De-hui Zhang, Yao Zheng, Hai-tao Sun
PURPOSE: To evaluate the effect of root canal therapy (RCT) of maxillary first molars and the relevant influential factors. METHODS: A total of 170 maxillary first molars undergoing endodontic treatment were retrospectively studied. 100 molars were treated with vertical compaction technique (VCT) using warm gutta- percha under dental operating microscope (DOM), while 70 molars with traditional lateral compaction technique (LCT) under naked eyes. All patients were asked to revisit 5 years later and 5-year success rate was evaluated...
February 2016: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
O V Grishchenko, K V Kiselev, G K Tchernoded, S A Fedoreyev, M V Veselova, V P Bulgakov, Y N Zhuravlev
Maackia amurensis Rupr. et Maxim is a valuable leguminous tree grown in the Russian Far East, in China, and in Korea. Polyphenols from the heartwood of this species (primarily stilbenes and isoflavonoids) possess strong hepatoprotective activity. Callus culture of M. amurensis produced isoflavonoids and their derivatives. In pharmacological experiments, the callus complex was at least as effective, as the plant complex. To increase the yield of isoflavonoids, calli were transformed with the rolB gene of Agrobacterium rhizogenes...
September 2016: Applied Microbiology and Biotechnology
Ololade Fatunmbi, Rinat R Abzalimov, Sergey N Savinov, Anne Gershenson, Igor A Kaltashov
Haptoglobin (Hp) binds free hemoglobin (Hb) dimers to prevent negative consequences of Hb circulation in the extracellular environment. Although both monomeric Hb and myoglobin (Mb) species also present potential risks, their interactions with Hp have not been extensively studied. Mb is homologous to both the α- and β-chains of Hb and shares many conserved Hb/Hp interface residues, yet whether Hp binds Mb remains unclear. To address this, computational biology tools were used to predict the interactions required for Hp to bind monomeric globins, and the predicted association was tested using native electrospray ionization mass spectrometry (ESI-MS)...
March 29, 2016: Biochemistry
Darshan B Dhakan, Rituja Saxena, Nikhil Chaudhary, Vineet K Sharma
Tepidimonas taiwanensis strain MB2 is a thermophile isolated from a hot spring located in central India. Here, we report a 28,49,160 bp draft genome sequence of Tepidimonas taiwanensis MB2. The genome shows properties of sulfur metabolism, nitrogen fixation, ammonia metabolism, assimilation of organic acids, and a wide variety of proteases.
2016: Genome Announcements
Jacob C Dean, Daniel G Oblinsky, Shahnawaz Rafiq, Gregory D Scholes
The photochemistry and aggregation properties of methylene blue (MB) lead to its popular use in photodynamic therapy. The facile formation of strongly coupled "face-to-face" H-aggregates in concentrated aqueous solution, however, significantly changes its spectroscopic properties and photophysics. The photoinitiated dynamics of the simplest MB aggregate, MB2, was investigated over femtosecond to nanosecond time scales revealing sequential internal conversion events that fully relax the excited population. MB monomer dynamics were analyzed in tandem for a direct comparison...
January 28, 2016: Journal of Physical Chemistry. B
Linling Zhu, Junying Zhang, Fengyang Wang, Ya Wang, Linlin Lu, Chongchong Feng, Zhiai Xu, Wen Zhang
Amyloid-beta (Aβ) oligomers are highly toxic species in the process of Aβ aggregation and are regarded as potent therapeutic targets and diagnostic markers for Alzheimer's disease (AD). Herein, a label-free molecular beacon (MB) system integrated with enzyme-free amplification strategy was developed for simple and highly selective assay of Aβ oligomers. The MB system was constructed with abasic site (AP site)-containing stem-loop DNA and a fluorescent ligand 2-amino-5,6,7-trimethyl-1,8-naphyridine (ATMND), of which the fluorescence was quenched upon binding to the AP site in DNA stem...
April 15, 2016: Biosensors & Bioelectronics
José de la Fuente, Iratxe Díez-Delgado, Marinela Contreras, Joaquín Vicente, Alejandro Cabezas-Cruz, Raquel Tobes, Marina Manrique, Vladimir López, Beatriz Romero, Javier Bezos, Lucas Dominguez, Iker A Sevilla, Joseba M Garrido, Ramón Juste, Guillermo Madico, Edward Jones-López, Christian Gortazar
Mycobacteria of the Mycobacterium tuberculosis complex (MTBC) greatly affect humans and animals worldwide. The life cycle of mycobacteria is complex and the mechanisms resulting in pathogen infection and survival in host cells are not fully understood. Recently, comparative genomics analyses have provided new insights into the evolution and adaptation of the MTBC to survive inside the host. However, most of this information has been obtained using M. tuberculosis but not other members of the MTBC such as M...
November 2015: PLoS Neglected Tropical Diseases
Benjamín Briseño-Marroquín, Frank Paqué, Karolin Maier, Brita Willershausen, Thomas Gerhard Wolf
INTRODUCTION: The objective of this study was to propose a root canal configuration description method and to investigate the root canal system morphology of the maxillary first molar by means of micro-computed tomographic imaging. METHODS: The root canal configuration, foramina, and accessory canal frequency of 179 maxillary first molars were investigated by means of micro-computed tomographic imaging and 3-dimensional software imaging. The root canal configuration and main foramina number are described from coronal to apical with a 4-digit system...
December 2015: Journal of Endodontics
Martin Dahlqvist, Ulf Jansson, Johanna Rosen
Transition metal diborides in hexagonal AlB2 type structure typically form stable MB2 phases for group IV elements (M  =  Ti, Zr, Hf). For group V (M  =  V, Nb, Ta) and group VI (M  =  Cr, Mo, W) the stability is reduced and an alternative hexagonal rhombohedral MB2 structure becomes more stable. In this work we investigate the effect of vacancies on the B-site in hexagonal MB2 and its influence on the phase stability and the structure for TiB2, ZrB2, HfB2, VB2, NbB2, TaB2, CrB2, MoB2, and WB2 using first-principles calculations...
November 4, 2015: Journal of Physics. Condensed Matter: An Institute of Physics Journal
Mario Luis Zuolo, Maria Cristina Carvalho, Gustavo De-Deus
The aim of this prospective case series report was to assess the frequency in which the Reciproc R25 instrument (VDW, Munich, Germany) is able to scout and reach the full working length (WL) in second mesiobuccal (MB2) canals from maxillary molars. Conventional hand file negotiation was used as the reference technique for comparison. Maxillary molars (270 first molars and 71 second molars) were included in this study. After local anesthesia, the tooth was isolated with a rubber dam, and traditional straight-line access was made...
November 2015: Journal of Endodontics
Rong Li, Ying Liu, Hanne Skovsgaard Pedersen, Henrik Callesen
Already at first embryo cleavage subsequent blastocyst formation can be predicted based on morphology but the finer morphological details can be difficult to determine due to the presence of the zona pellucida (ZP). Therefore, we monitored zona-free porcine parthenogenetically activated (PA) embryos in a time-lapse system to: (1) describe and characterise the morphological activity of the cytoplasmic membrane and the distribution to the two nuclei during first cleavage and (2) determine the relationship between specific morphological activities and subsequent embryonic development...
September 22, 2015: Reproduction, Fertility, and Development
Mohamed Abdel-Fattah, Ragia Charmy, Abdel-Rahman Tawfik
We have studied the immune reactivity of hypervariable region 1 (HVR1) of hepatitis C virus (HCV) against HCV immune positive and negative sera. Two published HVR1 consensus nucleotide sequences (Italian and Chinese) were synthesized, and with both of them, a splicing by overlap extension polymerase chain reaction, cloning and sequencing were performed. From the corresponding amino acid sequences, 3 Italian and 1 Chinese HVR1 peptides were selected for synthesis. The 4 peptides (MB1-MB4; GenBank No.: HQ846888-HQ846891) were used to screen 47 and 31 HCV (type 4) immune positive and negative sera, respectively, by enzyme-linked immunosorbent assay (ELISA)...
2015: Intervirology
Pablo Betancourt, Pablo Navarro, Mario Cantín, Ramón Fuentes
The maxillary second molar (2 MM) has the most complex root canal system, and a high percentage of treatment failures is due to the impossibility of locating, instrumenting and obturating the second mesiobuccal canal (MB2). The aim of this study was to describe in vivo the prevalence and location of the MB2 canal in the mesiobuccal root of the permanent maxillary second molar through CBCT image analysis. Two hundred twenty five CBCT images of 2 MM were studied. In the presence of the MB2 canal, the floor of the coronal cavity was located and advanced every 1 mm apically to standardize observation...
2015: International Journal of Clinical and Experimental Medicine
Hesam Mirmohammadi, Lamees Mahdi, Poerya Partovi, Abbasali Khademi, Hagay Shemesh, Bassam Hassan
INTRODUCTION: There is a high incidence of a second mesiobuccal root canal (MB2) in maxillary molars. The study aim is to assess the accuracy of cone-beam computed tomographic (CBCT) imaging in detecting a second mesiobuccal root canal in endodontically treated maxillary molars. METHODS: The study sample consisted of 60 extracted maxillary molars, which were endodontically opened and examined under a dental operating microscope for the presence of an MB2. The teeth were then further examined using micro-computed tomographic imaging as the gold standard...
October 2015: Journal of Endodontics
Yong Qian, Fenglei Gao, Lili Du, Yu Zhang, Daoquan Tang, Dongzhi Yang
In this work, we presented a novel label-free and enzyme-free electrochemical aptasensor based on DNA in situ silver metallization as effective electrochemical label. Molecular beacon 2 (MB2, Peptide nucleic acid) was first immobilized on the gold electrode (AuE) through Au-S bond. In the presence of thrombin, the thrombin binding aptamer (MB1) preferred to form thrombin/aptamer complex in lieu of aptamer-DNA duplex, resulting in the 8-17 DNAzyme liberating from the caged structure and hybridization with the MB2, the MB2 will replace and free the target thrombin when it hybridizes with MB1...
December 15, 2015: Biosensors & Bioelectronics
José de la Fuente, Iratxe Díez-Delgado, Marinela Contreras, Joaquín Vicente, Alejandro Cabezas-Cruz, Marina Manrique, Raquel Tobes, Vladimir López, Beatriz Romero, Lucas Domínguez, Joseba M Garrido, Ramón Juste, Christian Gortazar
Here we report the complete genome sequences of field isolates of Mycobacterium bovis and the related mycobacterial species, Mycobacterium caprae. The genomes of three M. bovis (MB1, MB3, MB4) and one M. caprae (MB2) field isolates with different virulence, prevalence, and host distribution phenotypes were sequenced.
2015: Genome Announcements
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