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https://www.readbyqxmd.com/read/28292597/the-effect-of-the-dental-operating-microscope-on-the-outcome-of-nonsurgical-root-canal-treatment-a-retrospective-case-control-study
#1
Navid Khalighinejad, Anita Aminoshariae, James C Kulild, Kristin A Williams, Jeannie Wang, Andre Mickel
INTRODUCTION: The aim of the current investigation was to assess the effect of the use of a dental operating microscope on the outcome of nonsurgical root canal treatment (NS RCT) while treating the mesiobuccal (MB) root of the maxillary first molar. METHODS: This retrospective investigation included endodontically treated maxillary first molars (ETMs) with apparent adequate previous NS RCT and restorations referred for endodontic retreatment at the endodontic graduate clinic...
March 11, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28194366/maxillary-first-molar-with-7-root-canals-diagnosed-using-cone-beam-computed-tomography
#2
Evaldo Rodrigues, Antônio Henrique Braitt, Bruno Ferraz Galvão, Emmanuel João Nogueira Leal da Silva
Root canal anatomy is complex, and the recognition of anatomic variations could be a challenge for clinicians. This case report describes the importance of cone beam computed tomographyic (CBCT) imaging during endodontic treatment. A 23 year old woman was referred by her general dental practitioner with the chief complaint of spontaneous pain in her right posterior maxilla. From the clinical and radiographic findings, a diagnosis of symptomatic irreversible pulpitis was made and endodontic treatment was suggested to the patient...
February 2017: Restorative Dentistry & Endodontics
https://www.readbyqxmd.com/read/28149463/a-cone-beam-computed-tomography-cbct-evaluation-of-mb2-canals-in-endodontically-treated-permanent-maxillary-molars-a-retrospective-study-in-indian-population
#3
Heeresh Shetty, Subodh Sontakke, Freny Karjodkar, Pankaj Gupta, Ashish Mandwe, K S Banga
BACKGROUND: Current technological advances have allowed application of different study designs and techniques for investigation of dental anatomy. Some clinical studies have provided evidence that Cone Beam computed tomography (CBCT) scanning is an important resource in assessment of root canal systems notably to identify MB2 canals in maxillary molars as CBCT scans allow in vivo dental investigation in axial, sagittal and coronal planes simultaneously. The current study was undertaken to detect and evaluate filled/unfilled MB2 canals in endodontically treated, asymptomatic maxillary molars utilizing cone beam computed tomography (CBCT)...
January 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28106044/root-canal-morphology-and-configuration-of-123-maxillary-second-molars-by-means-of-micro-ct
#4
Thomas Gerhard Wolf, Frank Paqué, Anja-Christin Woop, Brita Willershausen, Benjamín Briseño-Marroquín
The aim of this study was to investigate the root canal configuration, accessory canals and number of main foramina of 123 maxillary second molars by means of micro-computed tomography. The teeth were scanned and reproduced with 3D software imaging. The root canal configuration and number of main foramina were evaluated by means of a four-digit system. The morphological complexity of human maxillary second molars is depicted by the number of accessory and connecting canals. The most frequently observed root canal configurations in the mesiobuccal root were 2-2-2/2 (19...
January 20, 2017: International Journal of Oral Science
https://www.readbyqxmd.com/read/28097063/interferon-%C3%AE-responses-to-plasmodium-falciparum-vaccine-candidate-antigens-decrease-in-the-absence-of-malaria-transmission
#5
Cyrus Ayieko, Bilha S Ogola, Lyticia Ochola, Gideon A M Ngwena, George Ayodo, James S Hodges, Gregory S Noland, Chandy C John
BACKGROUND: Malaria elimination campaigns are planned or active in many countries. The effects of malaria elimination on immune responses such as antigen-specific IFN- γ responses are not well characterized. METHODS: IFN- γ responses to the P. falciparum antigens circumsporozoite protein, liver stage antigen-1, thrombospondin-related adhesive protein, apical membrane antigen-1, MB2, and merozoite surface protein-1 were tested by ELISA in 243 individuals in highland Kenya in April 2008, October 2008, and April 2009, after a one-year period of interrupted malaria transmission from April 2007 to March 2008...
2017: PeerJ
https://www.readbyqxmd.com/read/28094878/characterization-of-cellulose-degrading-bacteria-from-the-larval-gut-of-the-white-grub-beetle-lepidiota-mansueta-coleoptera-scarabaeidae
#6
Gautam Handique, Amrita Phukan, Badal Bhattacharyya, Abu Adil Lutful Haque Baruah, Syed Wasifur Rahman, Rajen Baruah
The goal of this study is to identify and characterize the cellulose degrading microorganisms in the larval gut of the white grub beetle, Lepidiota mansueta. Thirty bacterial strains were isolated and tested for cellulolytic activity using soluble carboxymethyl cellulose (CMC) degrading assays. Of these strains, five (FGB1, FB2, MB1, MB2, and HB1) degrade cellulose. Cellulolytic activity was determined based on formation of clear zone and cellulolytic index on CMC plate media. The highest cellulolytic index (2...
February 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/27994970/in-vitro-gastrointestinal-digestion-study-of-a-novel-bio-tofu-with-special-emphasis-on-the-impact-of-microbial-transglutaminase
#7
Guangliang Xing, Xin Rui, Mei Jiang, Yu Xiao, Ying Guan, Dan Wang, Mingsheng Dong
We have developed a novel bio-tofu, made from mixed soy and cow milk (MSCM), using Lactobacillus helveticus MB2-1 and Lactobacillus plantarum B1-6 incorporated with microbial transglutaminase (MTGase) as coagulant. MTGase was added to improve the textural properties and suit for cooking. However, the effect of MTGase on the digestion of mixed-protein fermented by lactic acid bacteria was unclear. This study aimed at evaluating the effect of MTGase on protein digestion of bio-tofu under simulated gastrointestinal digestion condition...
2016: PeerJ
https://www.readbyqxmd.com/read/27977863/cbct-uses-in-clinical-endodontics-the-effect-of-cbct-on-the-ability-to-locate-mb2-canals-in-maxillary-molars
#8
J Parker, A Mol, E M Rivera, P Tawil
AIM: To determine if a CBCT volume can aid in the location of MB2 canals in maxillary molars. METHODOLOGY: This prospective clinical study involved 50 patients that needed RCT on a maxillary molar. The teeth where the MB2 was located upon access with the dental operating microscope received routine root canal treatment, and teeth where MB2 was not located had a CBCT volume made after instrumenting the located canals. At the second appointment, the clinician used the aid of the CBCT volume and troughing to attempt to locate MB2...
December 15, 2016: International Endodontic Journal
https://www.readbyqxmd.com/read/27908285/prevalence-and-location-of-the-secondary-mesiobuccal-canal-in-1-100-maxillary-molars-using-cone-beam-computed-tomography
#9
Pablo Betancourt, Pablo Navarro, Gonzalo Muñoz, Ramón Fuentes
BACKGROUND: Several articles have used cone beam computed tomography (CBCT) to study the morphology of the maxillary molars and to ascertain its ability to visualize the second mesiobuccal canal (MB2); however, its geometric location has not been examined in depth. The aim of this study was to describe in vivo the prevalence and location of the MB2 in the mesiobuccal root of the first maxillary molar (1MM) and the second maxillary molar (2MM) through CBCT imaging. METHODS: Five hundred fifty CBCT images of the 1MM and 550 of the 2MM were analyzed...
December 1, 2016: BMC Medical Imaging
https://www.readbyqxmd.com/read/27895867/detection-of-second-mesiobuccal-canals-in-maxillary-first-molars-using-a-new-angle-of-cone-beam-computed-tomography
#10
Ali Murat Aktan, Cihan Yildirim, Emre Culha, Erhan Demir, Mehmet Ertugrul Ciftci
BACKGROUND: The localization of the additional canal orifice is one of the primary factors influencing the success of endodontic treatment. To deal with this problem, several techniques that each have their own advantages and disadvantages have been discussed in the literature. OBJECTIVES: The aim of the present in vitro study was to review a new approach to localizing second mesiobuccal (MB2) canals in maxillary first molars using cone beam computed tomography (CBCT)...
October 2016: Iranian Journal of Radiology: a Quarterly Journal Published By the Iranian Radiological Society
https://www.readbyqxmd.com/read/27628090/quencher-free-fluorescence-strategy-for-detection-of-dna-methyltransferase-activity-based-on-exonuclease-iii-assisted-signal-amplification
#11
Haisheng Liu, Changbei Ma, Meijuan Zhou, Hanchun Chen, Hailun He, Kemin Wang
This work demonstrates a novel method for DNA methyltransferase (MTase) activity detection with a quencher-free molecular beacon (MB) probe based on exonuclease (Exo) III-assisted signal amplification. In the presence of Dam MTase and DpnI endonuclease, the elaborately designed hairpin substrate (MB1) was cleaved into two parts (part A and part B). Exo III can then digest part A and release a single-stranded target of the 2-aminopurine-labeled MB (MB2). Subsequently, the MB2 can hybridize with its target to form a double-stranded structure with a protruding 3'-terminus and then trigger the digestion of MB2 by Exo III...
November 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27512111/unusual-diversity-of-myoglobin-genes-in-the-lungfish
#12
Jonas Koch, Julia Lüdemann, Rieke Spies, Marco Last, Chris T Amemiya, Thorsten Burmester
Myoglobin is a respiratory protein that serves as a model system in a variety of biological fields. Its main function is to deliver and store O2 in the heart and skeletal muscles, but myoglobin is also instrumental in homeostasis of nitric oxide (NO) and detoxification of reactive oxygen species (ROS). Almost every vertebrate harbors a single myoglobin gene; only some cyprinid fishes have two recently duplicated myoglobin genes. Here we show that the West African lungfish Protopterus annectens has at least seven distinct myoglobin genes (PanMb1-7), which diverged early in the evolution of lungfish and showed an enhanced evolutionary rate...
December 2016: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/27406904/influence-of-metal-diboride-and-dy2o3-additions-on-microstructure-and-properties-of-mgb2-fabricated-at-high-temperatures-and-under-pressure
#13
Y Yang, M D Sumption, E W Collings
High temperatures and under pressure (HTP) processing has been used to study the effects of chemical doping in MgB2. ZrB2, TiB2 and NbB2 were selected as additives since, like MgB2, they have an AlB2-type structure and similar lattice parameters. Dy2O3 was selected as it has been reported to generate nanoscale, secondary intragrain phases in MgB2. While C is known to enter the B-sublattice readily, attempts to dope Zr and other elements onto the Mg site have been less successful due to slow bulk diffusion, low solubility in MgB2, or both...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27331367/inflammation-induced-by-inhaled-lipopolysaccharide-depends-on-particle-size-in-healthy-volunteers
#14
Virginie Doyen, Gabrielle Pilcer, Phong Huy Duc Dinh, Francis Corazza, Alfred Bernard, Pierre Bergmann, Nicolas Lefevre, Karim Amighi, Olivier Michel
AIMS: In drug development, the anti-inflammatory properties of new molecules in the lung are currently tested using the inhaled lipopolysaccharide (LPS) model. The total and regional lung bioavailability of inhaled particles depends significantly on their size. The objective of the present study was to compare inflammatory responses in healthy volunteers after the inhalation of LPS of varying droplet size. METHODS: Three nebulizers were characterized by different droplet size distributions [mean mass median aerodynamic diameters: Microcirrus (2...
November 2016: British Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/27063316/-a-retrospective-clinical-analysis-of-endodontic-treatment-of-maxillary-first-molars
#15
De-hui Zhang, Yao Zheng, Hai-tao Sun
PURPOSE: To evaluate the effect of root canal therapy (RCT) of maxillary first molars and the relevant influential factors. METHODS: A total of 170 maxillary first molars undergoing endodontic treatment were retrospectively studied. 100 molars were treated with vertical compaction technique (VCT) using warm gutta- percha under dental operating microscope (DOM), while 70 molars with traditional lateral compaction technique (LCT) under naked eyes. All patients were asked to revisit 5 years later and 5-year success rate was evaluated...
February 2016: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/27063013/rolb-gene-induced-production-of-isoflavonoids-in-transformed-maackia-amurensis-cells
#16
O V Grishchenko, K V Kiselev, G K Tchernoded, S A Fedoreyev, M V Veselova, V P Bulgakov, Y N Zhuravlev
Maackia amurensis Rupr. et Maxim is a valuable leguminous tree grown in the Russian Far East, in China, and in Korea. Polyphenols from the heartwood of this species (primarily stilbenes and isoflavonoids) possess strong hepatoprotective activity. Callus culture of M. amurensis produced isoflavonoids and their derivatives. In pharmacological experiments, the callus complex was at least as effective, as the plant complex. To increase the yield of isoflavonoids, calli were transformed with the rolB gene of Agrobacterium rhizogenes...
September 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/26937685/interactions-of-haptoglobin-with-monomeric-globin-species-insights-from-molecular-modeling-and-native-electrospray-ionization-mass-spectrometry
#17
Ololade Fatunmbi, Rinat R Abzalimov, Sergey N Savinov, Anne Gershenson, Igor A Kaltashov
Haptoglobin (Hp) binds free hemoglobin (Hb) dimers to prevent negative consequences of Hb circulation in the extracellular environment. Although both monomeric Hb and myoglobin (Mb) species also present potential risks, their interactions with Hp have not been extensively studied. Mb is homologous to both the α- and β-chains of Hb and shares many conserved Hb/Hp interface residues, yet whether Hp binds Mb remains unclear. To address this, computational biology tools were used to predict the interactions required for Hp to bind monomeric globins, and the predicted association was tested using native electrospray ionization mass spectrometry (ESI-MS)...
March 29, 2016: Biochemistry
https://www.readbyqxmd.com/read/26893423/draft-genome-sequence-of-tepidimonas-taiwanensis-strain-mb2-a-chemolithotrophic-thermophile-isolated-from-a-hot-spring-in-central-india
#18
Darshan B Dhakan, Rituja Saxena, Nikhil Chaudhary, Vineet K Sharma
Tepidimonas taiwanensis strain MB2 is a thermophile isolated from a hot spring located in central India. Here, we report a 28,49,160 bp draft genome sequence of Tepidimonas taiwanensis MB2. The genome shows properties of sulfur metabolism, nitrogen fixation, ammonia metabolism, assimilation of organic acids, and a wide variety of proteases.
2016: Genome Announcements
https://www.readbyqxmd.com/read/26781668/methylene-blue-exciton-states-steer-nonradiative-relaxation-ultrafast-spectroscopy-of-methylene-blue-dimer
#19
Jacob C Dean, Daniel G Oblinsky, Shahnawaz Rafiq, Gregory D Scholes
The photochemistry and aggregation properties of methylene blue (MB) lead to its popular use in photodynamic therapy. The facile formation of strongly coupled "face-to-face" H-aggregates in concentrated aqueous solution, however, significantly changes its spectroscopic properties and photophysics. The photoinitiated dynamics of the simplest MB aggregate, MB2, was investigated over femtosecond to nanosecond time scales revealing sequential internal conversion events that fully relax the excited population. MB monomer dynamics were analyzed in tandem for a direct comparison...
January 28, 2016: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/26613510/selective-amyloid-%C3%AE-oligomer-assay-based-on-abasic-site-containing-molecular-beacon-and-enzyme-free-amplification
#20
Linling Zhu, Junying Zhang, Fengyang Wang, Ya Wang, Linlin Lu, Chongchong Feng, Zhiai Xu, Wen Zhang
Amyloid-beta (Aβ) oligomers are highly toxic species in the process of Aβ aggregation and are regarded as potent therapeutic targets and diagnostic markers for Alzheimer's disease (AD). Herein, a label-free molecular beacon (MB) system integrated with enzyme-free amplification strategy was developed for simple and highly selective assay of Aβ oligomers. The MB system was constructed with abasic site (AP site)-containing stem-loop DNA and a fluorescent ligand 2-amino-5,6,7-trimethyl-1,8-naphyridine (ATMND), of which the fluorescence was quenched upon binding to the AP site in DNA stem...
April 15, 2016: Biosensors & Bioelectronics
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