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M Leo, L Bartalena, G Rotondo Dottore, E Piantanida, P Premoli, I Ionni, M Di Cera, E Masiello, L Sassi, M L Tanda, F Latrofa, P Vitti, C Marcocci, M Marinò
OBJECTIVE: In spite of previous conflicting results, an adjuvant role of selenium in the treatment of Graves' disease (GD) hyperthyroidism has been proposed. To address this issue, a randomized clinical trial was carried out aimed at investigating whether selenium is beneficial on the short-term control of GD hyperthyroidism treated with methimazole (MMI). METHODS: Thirty newly diagnosed hyperthyroid GD patients were randomly assigned to treatment with: (i) MMI or (ii) MMI plus selenium...
October 12, 2016: Journal of Endocrinological Investigation
James E M Lewis, Joby Winn, Luca Cera, Stephen M Goldup
We present an operationally simple iterative coupling strategy for the synthesis of oligomeric homo- and hetero-[n]rotaxanes with precise control over the position of each macrocycle. The exceptional yield of the AT-CuAAC reaction, combined with optimized conditions that allow the rapid synthesis of the target oligomers, opens the door to the study of precision-engineered oligomeric interlocked molecules.
October 4, 2016: Journal of the American Chemical Society
Christopher S Eickhoff, Xiuli Zhang, Jose R Vasconcelos, R Geoffrey Motz, Nicole L Sullivan, Kelly O'Shea, Nicola Pozzi, David W Gohara, Jennifer R Blase, Enrico Di Cera, Daniel F Hoft
Trypanosoma cruzi infection is controlled but not eliminated by host immunity. The T. cruzi trans-sialidase (TS) gene superfamily encodes immunodominant protective antigens, but expression of altered peptide ligands by different TS genes has been hypothesized to promote immunoevasion. We molecularly defined TS epitopes to determine their importance for protection versus parasite persistence. Peptide-pulsed dendritic cell vaccination experiments demonstrated that one pair of immunodominant CD4+ and CD8+ TS peptides alone can induce protective immunity (100% survival post-lethal parasite challenge)...
September 2016: PLoS Pathogens
Stephen B Keysar, Phuong N Le, Bettina Miller, Brian C Jackson, Justin R Eagles, Cera Nieto, Jihye Kim, Binwu Tang, Magdalena J Glogowska, J Jason Morton, Nuria Padilla-Just, Karina Gomez, Emily Warnock, Julie Reisinger, John J Arcaroli, Wells A Messersmith, Lalage M Wakefield, Dexiang Gao, Aik-Choon Tan, Hilary Serracino, Antonio Jimeno
BACKGROUND: We have an incomplete understanding of the differences between cancer stem cells (CSCs) in human papillomavirus-positive (HPV-positive) and -negative (HPV-negative) head and neck squamous cell cancer (HNSCC). The PI3K pathway has the most frequent activating genetic events in HNSCC (especially HPV-positive driven), but the differential signaling between CSCs and non-CSCs is also unknown. METHODS: We addressed these unresolved questions using CSCs identified from 10 HNSCC patient-derived xenografts (PDXs)...
January 2017: Journal of the National Cancer Institute
Mark R Gillrie, Bernard Renaux, Eleanor Russell-Goldman, Marion Avril, Andrew J Brazier, Koichiro Mihara, Enrico Di Cera, Danny A Milner, Morley D Hollenberg, Joseph D Smith, May Ho
UNLABELLED: Plasmodium falciparum malaria remains one of the most deadly infections worldwide. The pathogenesis of the infection results from the sequestration of infected erythrocytes (IRBC) in vital organs, including the brain, with resulting impairment of blood flow, hypoxia, and lactic acidosis. Sequestration occurs through the adhesion of IRBC to host receptors on microvascular endothelium by Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1), a large family of variant surface antigens, each with up to seven extracellular domains that can bind to multiple host receptors...
2016: MBio
Beth Careyva, Kyle Shaak, Geoffrey Mills, Melanie Johnson, Samantha Goodrich, Brian Stello, Lorraine S Wallace
BACKGROUND: Technology-based patient engagement strategies (such as patient portals) are increasingly available, yet little is known about current use and barriers within practice-based research networks (PBRNs). PBRN directors have unique opportunities to inform the implementation of patient-facing technology and to translate these findings into practice. METHODS: PBRN directors were queried regarding technology-based patient engagement strategies as part of the 2015 CAFM Educational Research Alliance (CERA) survey of PBRN directors...
September 2016: Journal of the American Board of Family Medicine: JABFM
Gianpiero Cera, Lutz Ackermann
Iron-catalyzed C-H activation has recently emerged as an increasingly powerful tool for the step-economical transformation of unreactive C-H bonds. Particularly, the recent development of low-valent iron catalysis has set the stage for novel C-H activation strategies via chelation assistance. The low-cost, natural abundance, and low toxicity of iron prompted its very recent application in organometallic C-H activation catalysis. An overview of the use of iron catalysis in C-H activation processes is summarized herein up to May 2016...
October 2016: Top Curr Chem (J)
Ghazaleh Mosleh, Parmis Badr
BACKGROUND: Qairooti (Cerate), a medicinal salve or ointment, compounded of wax and oil, is a formulation used alone or as a basis for medicinal dosage forms. It is widely used from the ancient times to the present. Based on its structure, beeswax has unique characteristics. It builds stable emulsions and increases water absorbance of creams and ointments. The aim of this study was to gather all pharmaceutical information about preparing Qairooti products from traditional pharmacopoeaes, such as the various types of Qairooti and their preparation methods...
May 2016: Iranian Journal of Medical Sciences
Ruben Hernandez, Javier F Sevilla Martir, Daniel J Van Durme, Meredith J Faller, Sabrina Yong-Yow, M Kelly Davies, Morhaf Al Achkar
BACKGROUND AND OBJECTIVES: Interest in global health (GH) has increased significantly among medical trainees in the past 3 decades. Despite the potential for family medicine to be a major contributor to GH, there are no recent, large-scale studies of GH education and experiences in family medicine training. This study was designed to assess current opportunities, educational activities, resident interest, perceived program benefits, and barriers to international and domestic GH training in US family medicine residencies...
July 2016: Family Medicine
David W Gohara, Enrico Di Cera
Regulation of enzymes through metal ion complexation is widespread in biology and underscores a physiological need for stability and high catalytic activity that likely predated proteins in the RNA world. In addition to divalent metals such as Ca(2+), Mg(2+), and Zn(2+), monovalent cations often function as efficient and selective promoters of catalysis. Advances in structural biology unravel a rich repertoire of molecular mechanisms for enzyme activation by Na(+) and K(+) Strategies range from short-range effects mediated by direct participation in substrate binding, to more distributed effects that propagate long-range to catalytic residues...
September 30, 2016: Journal of Biological Chemistry
Keitaro Nishizawa, Toshiyuki Yano, Masaya Tanno, Takayuki Miki, Atsushi Kuno, Toshiyuki Tobisawa, Makoto Ogasawara, Shingo Muratsubaki, Kouhei Ohno, Satoko Ishikawa, Tetsuji Miura
Chronic kidney disease (CKD) is known to increase myocardial infarct size after ischemia/reperfusion. However, a strategy to prevent the CKD-induced myocardial susceptibility to ischemia/reperfusion injury has not been developed. Here, we examined whether epoetin β pegol, a continuous erythropoietin receptor activator (CERA), normalizes myocardial susceptibility to ischemia/reperfusion injury by its effects on protective signaling and metabolomes in CKD. CKD was induced by 5/6 nephrectomy in rats (subtotal nephrectomy, SNx), whereas sham-operated rats served controls (Sham)...
September 2016: Hypertension
Francesco Berardinelli, Piergustavo De Francesco, Michele Marchioni, Nicoletta Cera, Silvia Proietti, Derek Hennessey, Orietta Dalpiaz, Cecilia Cracco, Cesare Scoffone, Luigi Schips, Guido Giusti, Luca Cindolo
PURPOSE: Retrograde intrarenal surgery (RIRS) is considered a safe procedure; however, infective complications are potentially serious postoperative complications. The aim of this multicentre study was to evaluate prospectively the prevalence of infective complications after RIRS and identify risk factors. METHODS: Baseline data were collected, and patients were questioned regarding postoperative infective complications following RIRS. The Fisher exact test, Student t test, Mann-Whitney U test, and multivariate regression analysis were used for data analysis...
November 2016: International Urology and Nephrology
Nicola Pozzi, Dominika Bystranowska, Xiaobing Zuo, Enrico Di Cera
The coagulation factor prothrombin has a complex spatial organization of its modular assembly that comprises the N-terminal Gla domain, kringle-1, kringle-2, and the C-terminal protease domain connected by three intervening linkers. Here we use single molecule Förster resonance energy transfer to access the conformational landscape of prothrombin in solution and uncover structural features of functional significance that extend recent x-ray crystallographic analysis. Prothrombin exists in equilibrium between two alternative conformations, open and closed...
August 26, 2016: Journal of Biological Chemistry
Wen-Sheng Liu, Da-Chen Chu, Hsiang-Lin Chan, Szu-Yuan Li, Chih-Kuang Liu, Chih-Yu Yang, Yu-Wei Chen, Pui-Ching Lee, Yen-Ting Lai, Chih-Ching Lin
Anaemia is an important issue in patients undergoing haemodialysis. We aimed to identify a better dosing schedule of a fixed monthly dose of continuous erythropoietin receptor activator (CERA) in patients with chronic kidney disease (CKD) on haemodialysis. The CERA dosing schedule included 100 μg once monthly for 2 months, 50 μg twice monthly for 2 months and then 100 μg once monthly for two months. The effectiveness was determined by comparing haematocrit, nutritional status (serum protein and albumin) and inflammatory markers (tumour necrosis factor (TNF)-α, interleukin (IL)-1, IL-6 and Hepcidin) at the beginning of the study with those at the end of the study...
October 2016: Clinical and Experimental Pharmacology & Physiology
N Pozzi, E Di Cera
UNLABELLED: Essentials Prothrombin converts slowly to thrombin upon interaction with histone H4. Histone H4 may also affect the reactivity of prothrombin toward factor Xa. Histone H4 enhances or inhibits activation by factor Xa depending on cofactor Va. The results reveal an unanticipated dual effect of histone H4 on prothrombin activation by factor Xa. SUMMARY: Background Recent studies have documented the ability of prothrombin to convert to the mature protease thrombin upon interaction with histone H4...
September 2016: Journal of Thrombosis and Haemostasis: JTH
Nicola Pozzi, Mirco Zerbetto, Laura Acquasaliente, Simone Tescari, Diego Frezzato, Antonino Polimeno, David W Gohara, Enrico Di Cera, Vincenzo De Filippis
Thrombin exists as an ensemble of active (E) and inactive (E*) conformations that differ in their accessibility to the active site. Here we show that redistribution of the E*-E equilibrium can be achieved by perturbing the electrostatic properties of the enzyme. Removal of the negative charge of the catalytic Asp102 or Asp189 in the primary specificity site destabilizes the E form and causes a shift in the 215-217 segment that compromises substrate entrance. Solution studies and existing structures of D102N document stabilization of the E* form...
July 19, 2016: Biochemistry
Yanzhuo Zhang, James L Hanula, Scott Horn, Cera Jones, S Kristine Braman, Jianghua Sun
Chinese privet, Ligustrum sinense Lour., is an invasive shrub within riparian areas of the southeastern United States. Biological control is considered the most suitable management option for Chinese privet. The potential host range of the lace bug, Leptoypha hospita Drake et Poor, was evaluated on the basis of adult feeding and oviposition, combined oviposition-nymphal development no-choice tests, nymphal development no-choice tests, multiple generation comparison on Forestiera pubescens Nutt. and L. sinense no-choice tests, and multiple-choice tests with 45 plant species in 13 families...
August 2016: Environmental Entomology
Susan Cochella, Winston Liaw, Juliann Binienda, Carol Hustedde
BACKGROUND AND OBJECTIVES: The Society of Teachers of Family Medicine's (STFM) National Clerkship Curriculum (NCC) was created to standardize and improve teaching of a minimum core curriculum in family medicine clerkships, promoting the Triple Aim of better care and population health at lower cost. It includes competencies all clerkships should teach and tools to support clerkship directors (CDs). This 2014 CERA survey of clerkship directors is one of several needs assessments that guide STFM's NCC Editorial Board in targeting improvements and peer-review processes...
June 2016: Family Medicine
Linda P Rouse, Shalena Gallagher-Garza, Roberta E Gebhard, Suzanne L Harrison, Lorraine S Wallace
BACKGROUND: Continuing gender disparities within the medical profession have raised concerns about the extent to which women physicians face an inhospitable work environment. The purpose of this study was to examine the types and frequency of workplace bullying reported by a national sample of family physicians employed in academic settings, as related to gender. MATERIAL AND METHODS: Data for this study were gathered as part of the Council of Academic Family Medicine (CAMF) Educational Research Alliance (CERA) omnibus electronic survey...
September 2016: Journal of Women's Health
Wen-Sheng Liu, Hsiang-Lin Chan, Yen-Ting Lai, Ya-Hsu Yang, Hao-Wei Teng, Chih-Kuang Liu, Szu-Yuan Li, Chih-Yu Yang, Tsung-Yun Liu, Chih-Ching Lin
OBJECTIVE: The response of erythropoietic stimulating agents (ESA) in uremic patients may be associated with the changes of biochemical parameters, metal elements and inflammation status during the shift from one ESA to another. METHOD: We compared changes in above mentioned factors after switching from darbepoetin-α (DPO) 20μg weekly for 10 weeks to continuous erythropoietin receptor activator (CERA) 100μg monthly for 10 weeks in uremic patients on hemodialysis...
July 2016: Environmental Toxicology and Pharmacology
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