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Allergen asthma challenge study

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https://www.readbyqxmd.com/read/27891156/usefulness-of-component-resolved-analysis-of-cat-allergy-in-routine-clinical-practice
#1
Katharina Eder, Sven Becker, Marion San Nicoló, Alexander Berghaus, Moritz Gröger
BACKGROUND: Cat allergy is of great importance, and its prevalence is increasing worldwide. Cat allergens and house dust mite allergens represent the major indoor allergens; however, they are ubiquitous. Cat sensitization and allergy are known risk factors for rhinitis, bronchial hyperreactivity and asthma. Thus, the diagnosis of sensitization to cats is important for any allergist. METHODS: 70 patients with positive skin prick tests for cats were retrospectively compared regarding their skin prick test results, as well as their specific immunoglobulin E antibody profiles with regard to their responses to the native cat extract, rFel d 1, nFel d 2 and rFel d 4...
2016: Allergy, Asthma, and Clinical Immunology
https://www.readbyqxmd.com/read/27854355/effects-of-asian-dust-particles-on-the-early-stage-antigen-induced-immune-response-of-asthma-in-nc-nga-mice
#2
Jun Kurai, Masanari Watanabe, Hiroyuki Sano, Degejirihu Hantan, Yuji Tohda, Eiji Shimizu
Asian dust (AD) can aggravate airway inflammation in asthma, but the association between AD and the development of asthma remains unclear. This study aimed to investigate the effects of AD on the early stage of antigen sensitization using a mouse model of asthma, as well as the role of leukotrienes (LTs) in antigen-induced airway inflammation potentiated by AD particles. NC/Nga mice were co-sensitized by intranasal instillation of AD particles and/or Dermatophagoides farinae (Df) for five consecutive days. Df-sensitized mice were stimulated with an intranasal Df challenge at seven days...
November 16, 2016: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/27847210/targeted-inhibition-of-six1-attenuates-allergic-airway-inflammation-and-remodeling-in-asthmatic-mice
#3
Zhao-Chuan Yang, Ming-Ji Yi, Yan-Chun Shan, Chong Wang, Ni Ran, Li-Ying Jin, Peng Fu, Xue-Ying Feng, Lei Xu, Zheng-Hai Qu
Asthma is an inflammatory disease of the airways, characterized by lung eosinophilia, mucus hypersecretion by goblet cells and airway hyperresponsiveness to inhaled allergens. The purpose of this study was to evaluate the effects of Six1 on airway inflammation and remodeling and the underlying mechanisms in a murine model of chronic asthma. Female BALB/c mice were randomly divided into four groups: phosphate-buffered saline control, ovalbumin (OVA)-induced asthma group, OVA+siNC and OVA+siSix1. In this mice model, Six1 expression level was significantly elevated in OVA-induced asthma of mice...
November 12, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27846830/overexpression-of-programmed-cell-death-5-in-a-mouse-model-of-ovalbumin-induced-allergic-asthma
#4
Xiaolin Diao, Juan Wang, Hong Zhu, Bei He
BACKGROUND: Programmed cell death 5 (PDCD5) was first identified as an apoptosis-promoting protein and involved in some autoimmune diseases and inflammatory processes. Our previous study demonstrated greater expression of serum PDCD5 in asthmatic patients than controls. This study aimed to further explore the significance of PDCD5 in mice with induced allergic asthma. METHODS: We divided 16 female mice into 2 groups: control (n = 8) and allergen (ovalbumin, OVA)-challenged mice (n = 8)...
November 15, 2016: BMC Pulmonary Medicine
https://www.readbyqxmd.com/read/27787629/lymphangiogenesis-in-rat-asthma-model
#5
Aigul Moldobaeva, John Jenkins, Qiong Zhong, Elizabeth M Wagner
Although bronchial angiogenesis has been well documented in allergic asthma, lymphangiogenesis has not been widely studied. Therefore, we evaluated changes in lung lymphatics in a rat model of allergen-induced asthma using house dust mite (Der p 1; 100 μg/challenge). Additionally, properties of isolated lung lymphatic endothelial cells (CD45(-), CD141(+), LYVE-1(+), Prox-1(+)) were studied in vitro. Three weeks after the onset of intranasal allergen exposure (twice-weekly), an increase in the number of lung lymphatic vessels was measured (34% increase) by lung morphometry...
October 27, 2016: Angiogenesis
https://www.readbyqxmd.com/read/27779664/inhalation-of-inactivated%C3%A2-mycobacterium%C3%A2-phlei-prevents-asthma%C3%A2-mediated-airway-hyperresponsiveness-and-airway-eosinophilia-in-mice-by-reducing-il%C3%A2-5-and-il%C3%A2-13-levels
#6
Moyu Ming, Zhixi Luo, Shengqiu Lv, Chaoqian Li
The present study aimed to investigate whether inhalation of inactivated‑Mycobacterium phlei could prevent airway hyperresponsiveness and airway eosinophilia. A total of 24 male Balb/c mice were randomly divided into three groups: Normal control group (group A), asthma model group (group B) and the intervention group (group C), (8 mice/group). Group A mice were sensitized and with challenged saline and group B with ovalbumin (OVA). Group C mice were administered with aerosol Mycobacterium phlei once daily prior to the allergen challenge...
October 19, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27746238/house-dust-mite-driven-asthma-and-allergen-specific-t-cells-depend-on-b-cells-when-the-amount-of-inhaled-allergen-is-limiting
#7
Melissa Dullaers, Martijn J Schuijs, Monique Willart, Kaat Fierens, Justine Van Moorleghem, Hamida Hammad, Bart N Lambrecht
BACKGROUND: Allergic asthma is a CD4 TH2-lymphocyte driven disease characterized by airway hyperresponsiveness and eosinophilia. B cells can present antigens to CD4 T cells and produce IgE immunoglobulins that arm effector cells; however, mouse models are inconclusive on whether B cells are necessary for asthma development. OBJECTIVES: We sought to address the role of B cells in a house dust mite (HDM)-driven TH2-high asthma mouse model. METHODS: Wild-type and B cell-deficient muMT mice were sensitized and challenged through the airways with HDM extracts...
October 13, 2016: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27743410/development-of-eosinophilic-inflammation-is-independent-of-b-t-cell-interaction-in-a-chronic-house-dust-mite-driven-asthma-model
#8
H Vroman, I M Bergen, B W S Li, J A C van Hulst, M Lukkes, D van Uden, R W Hendriks, M Kool
BACKGROUND: Chronic exposure to environmental triggers, such as house dust mite (HDM), drives T helper 2 (Th2) cell-mediated asthma. Recent evidence has shown that B-T cell interaction, and in particular germinal center reactions and follicular T helper (Tfh) cells are required for the development of eosinophilic airway inflammation in HDM-driven models containing a sensitization and challenge phase. Whether B-T cell interactions are essential for pulmonary eosinophilic inflammation following chronic allergen provocation remains unknown...
October 15, 2016: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27733553/blomia-tropicalis-specific-tcr-transgenic-th2-cells-induce-inducible-balt-and-severe-asthma-in-mice-by-an-il-4-il-13-dependent-mechanism
#9
Yen Leong Chua, Ka Hang Liong, Chiung-Hui Huang, Hok Sum Wong, Qian Zhou, Say Siong Ler, Yafang Tang, Chin Pei Low, Hui Yu Koh, I-Chun Kuo, Yongliang Zhang, W S Fred Wong, Hong Yong Peh, Hwee Ying Lim, Moyar Qing Ge, Angela Haczku, Veronique Angeli, Paul A MacAry, Kaw Yan Chua, David M Kemeny
Previous studies have highlighted the importance of lung-draining lymph nodes in the respiratory allergic immune response, whereas the lung parenchymal immune system has been largely neglected. We describe a new in vivo model of respiratory sensitization to Blomia tropicalis, the principal asthma allergen in the tropics, in which the immune response is focused on the lung parenchyma by transfer of Th2 cells from a novel TCR transgenic mouse, specific for the major B. tropicalis allergen Blo t 5, that targets the lung rather than the draining lymph nodes...
November 15, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27685606/allergen-induced-increases-in-interleukin-25-and-interleukin-25-receptor-expression-in-mature-eosinophils-from-atopic-asthmatics
#10
Wei Tang, Steven G Smith, Brittany Salter, John Paul Oliveria, Patrick Mitchell, Graeme M Nusca, Karen Howie, Gail M Gauvreau, Paul M O'Byrne, Roma Sehmi
BACKGROUND: Interleukin (IL)-25 plays a pivotal role in type 2 immune responses. In a baseline cross-sectional study, we previously showed that IL-25 plasma levels and IL-25 receptor (IL-25R: IL-17RA, IL-17RB, and IL-17RA/RB) expression on mature blood eosinophils are increased in atopic asthmatics compared to normal nonatopic controls. This study investigated allergen-induced changes in IL-25 and IL-25R expression in eosinophils from asthmatics. METHODS: Dual responder atopic asthmatics (n = 14) were enrolled in this randomized diluent-controlled crossover allergen challenge study...
2016: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/27633092/adenovirus-mediated-foxp3-expression-in-lung-epithelial-cells-reduces-airway-inflammation-in-ovalbumin-and-cockroach-induced-asthma-model
#11
Soojin Park, Hwan-Suck Chung, Dasom Shin, Kyung-Hwa Jung, Hyunil Lee, Junghee Moon, Hyunsu Bae
Foxp3 is a master regulator of CD4(+)CD25(+) regulatory T-cell (Treg) function and is also a suppressor of SKP2 and HER2/ErbB2. There are an increasing number of reports describing the functions of Foxp3 in cell types other than Tregs. In this context, we evaluated the functions of Foxp3 in ovalbumin- and cockroach-induced asthma models. Foxp3-EGFP-expressing adenovirus or EGFP control adenovirus was administered intratracheally (i.t.), followed by challenge with ovalbumin (OVA) or cockroach extract to induce asthma...
September 16, 2016: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/27602069/interstitial-collagen-turnover-during-airway-remodeling-in-acute-and-chronic-experimental-asthma
#12
Georgina González-Avila, Blanca Bazan-Perkins, Cuauhtémoc Sandoval, Bettina Sommer, Sebastian Vadillo-Gonzalez, Carlos Ramos, Arnoldo Aquino-Galvez
Asthma airway remodeling is characterized by the thickening of the basement membrane (BM) due to an increase in extracellular matrix (ECM) deposition, which contributes to the irreversibility of airflow obstruction. Interstitial collagens are the primary ECM components to be increased during the fibrotic process. The aim of the present study was to examine the interstitial collagen turnover during the course of acute and chronic asthma, and 1 month after the last exposure to the allergen. Guinea pigs sensitized to ovalbumin (OVA) and exposed to 3 further OVA challenges (acute model) or 12 OVA challenges (chronic model) were used as asthma experimental models...
September 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27587397/eosinophil-peroxidase-catalyzed-protein-carbamylation-participates-in-asthma
#13
Zeneng Wang, Joseph A DiDonato, Jennifer Buffa, Suzy A Comhair, Mark A Aronica, Raed A Dweik, Nancy A Lee, James J Lee, Mary Jane Thomassen, Mani Kavuru, Serpil C Erzurum, Stanley L Hazen
The biochemical mechanisms through which eosinophils contribute to asthma pathogenesis are unclear. Here we show eosinophil peroxidase (EPO), an abundant granule protein released by activated eosinophils, contributes to characteristic asthma-related phenotypes through oxidative posttranslational modification (PTM) of proteins in asthmatic airways through a process called carbamylation. Using a combination of studies we now show EPO uses plasma levels of the pseudohalide thiocyanate (SCN(-)) as substrate to catalyze protein carbamylation, as monitored by PTM of protein lysine residues into N(ϵ)-carbamyllysine (homocitrulline), and contributes to the pathophysiological sequelae of eosinophil activation...
October 14, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27554816/gene-based-analysis-of-regulatory-variants-identifies-four-putative-novel-asthma-risk-genes-related-to-nucleotide-synthesis-and-signaling
#14
Manuel A R Ferreira, Rick Jansen, Gonneke Willemsen, Brenda Penninx, Lisa M Bain, Cristina T Vicente, Joana A Revez, Melanie C Matheson, Jennie Hui, Joyce Y Tung, Svetlana Baltic, Peter Le Souëf, Grant W Montgomery, Nicholas G Martin, Colin F Robertson, Alan James, Philip J Thompson, Dorret I Boomsma, John L Hopper, David A Hinds, Rhiannon B Werder, Simon Phipps
BACKGROUND: Hundreds of genetic variants are thought to contribute to variation in asthma risk by modulating gene expression. Methods that increase the power of genome wide association studies (GWAS) to identify risk-associated variants are needed. OBJECTIVE: To develop a method that aggregates the evidence for association with disease risk across expression quantitative trait loci (eQTLs) of a gene and use this approach to identify asthma risk genes. METHODS: We developed a gene-based test and software package called EUGENE that (1) is applicable to GWAS summary statistics; (2) considers both cis- and trans-eQTLs; (3) incorporates eQTLs identified in different tissues; and (4) uses simulations to account for multiple testing...
August 20, 2016: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27472490/following-up-infant-bronchiolitis-patients-provided-new-evidence-for-and-against-the-united-airway-disease-hypothesis
#15
Eero Lauhkonen, Petri Koponen, Kirsi Nuolivirta, Merja Helminen, Marita Paassilta, Jyri Toikka, Matti Korppi
AIM: The united airway disease (UAD) hypothesis suggests that allergic rhinitis and asthma develop together. We evaluated the evidence for and against the UAD hypothesis at five to seven years of age after hospitalisation for bronchiolitis at less than six months. METHODS: This study used prospective follow-up data for 102 children hospitalised for bronchiolitis under the age of six months. We included the presence of previous and current asthma, prolonged rhinitis and skin prick tests (SPT) to common inhaled allergens and lung function by impulse oscillometry (IOS) at five to seven years of age...
November 2016: Acta Paediatrica
https://www.readbyqxmd.com/read/27457925/regulation-of-eosinophilia-and-allergic-airway-inflammation-by-the-glycan-binding-protein-galectin-1
#16
Xiao Na Ge, Sung Gil Ha, Yana G Greenberg, Amrita Rao, Idil Bastan, Ada G Blidner, Savita P Rao, Gabriel A Rabinovich, P Sriramarao
Galectin-1 (Gal-1), a glycan-binding protein with broad antiinflammatory activities, functions as a proresolving mediator in autoimmune and chronic inflammatory disorders. However, its role in allergic airway inflammation has not yet been elucidated. We evaluated the effects of Gal-1 on eosinophil function and its role in a mouse model of allergic asthma. Allergen exposure resulted in airway recruitment of Gal-1-expressing inflammatory cells, including eosinophils, as well as increased Gal-1 in extracellular spaces in the lungs...
August 16, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27456482/prostaglandin-i2-suppresses-proinflammatory-chemokine-expression-cd4-t-cell-activation-and-stat6-independent-allergic-lung-inflammation
#17
Weisong Zhou, Jian Zhang, Kasia Goleniewska, Daniel E Dulek, Shinji Toki, Dawn C Newcomb, Jacqueline Y Cephus, Robert D Collins, Pingsheng Wu, Mark R Boothby, R Stokes Peebles
Allergic airway diseases are immune disorders associated with heightened type 2 immune responses and IL-5 and IL-13 production at the site of inflammation. We have previously reported that cyclooxygenase (COX) inhibition by indomethacin augmented allergic airway inflammation in a STAT6-independent manner. However, the key COX product(s) responsible for restraining indomethacin-mediated STAT6-independent allergic inflammation is unknown. In this study, using the mouse model of OVA-induced allergic airway inflammation, we identified that PGI2 receptor (IP) signaling was critical for indomethacin-induced, STAT6-independent proallergic effects...
September 1, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27449512/plethysmography-phenotype-qtl-in-mice-before-and-after-allergen-sensitization-and-challenge
#18
Samir N P Kelada
Allergic asthma is common airway disease that is characterized in part by enhanced airway constriction in response to nonspecific stimuli. Genome-wide association studies have identified multiple loci associated with asthma risk in humans, but these studies have not accounted for gene-environment interactions, which are thought to be important factors in asthma. To identify quantitative trait loci (QTL) that regulate responses to a common human allergen, we applied a house dust mite mouse (HDM) model of allergic airway disease (AAD) to 146 incipient lines of the Collaborative Cross (CC) and the CC founder strains...
September 8, 2016: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/27444660/a-dual-cyslt1-2-antagonist-attenuates-allergen-induced-airway-responses-in-subjects-with-mild-allergic-asthma
#19
G M Gauvreau, L-P Boulet, J M FitzGerald, D W Cockcroft, B E Davis, R Leigh, M Tanaka, J A Fourre, M Tanaka, T Nabata, P M O'Byrne
BACKGROUND: The cysteinyl leukotrienes (cysLTs) play a key role in the pathophysiology of asthma. In addition to functioning as potent bronchoconstrictors, cysLTs contribute to airway inflammation through eosinophil and neutrophil chemotaxis, plasma exudation, and mucus secretion. We tested the activity of the dual cysLT1/2 antagonist, ONO-6950, against allergen-induced airway responses. METHODS: Subjects with documented allergen-induced early (EAR) and late asthmatic response (LAR) were randomized in a three-way crossover study to receive ONO-6950 (200 mg) or montelukast (10 mg) or placebo q...
December 2016: Allergy
https://www.readbyqxmd.com/read/27421833/sputum-rna-signature-in-allergic-asthmatics-following-allergen-bronchoprovocation-test
#20
Rob G J A Zuiker, Catherine Tribouley, Zuzana Diamant, J Diderik Boot, Adam F Cohen, K Van Dyck, I De Lepeleire, Veronica M Rivas, Vladislav A Malkov, Jacobus Burggraaf, Marcella K Ruddy
BACKGROUND: Inhaled allergen challenge is a validated disease model of allergic asthma offering useful pharmacodynamic assessment of pharmacotherapeutic effects in a limited number of subjects. OBJECTIVES: To evaluate whether an RNA signature can be identified from induced sputum following an inhaled allergen challenge, whether a RNA signature could be modulated by limited doses of inhaled fluticasone, and whether these gene expression profiles would correlate with the clinical endpoints measured in this study...
2016: European Clinical Respiratory Journal
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