keyword
MENU ▼
Read by QxMD icon Read
search

TRF1

keyword
https://www.readbyqxmd.com/read/27836746/trf2-recruits-orc-through-trfh-domain-dimerization
#1
Mitsunori Higa, Tatsunori Kushiyama, Seiichiro Kurashige, Daisuke Kohmon, Kouki Enokitani, Satoko Iwahori, Nozomi Sugimoto, Kazumasa Yoshida, Masatoshi Fujita
Telomeres are specialized chromatin structures that prevent the degradation and instability of the ends of linear chromosomes. While telomerase maintains long stretches of the telomeric repeat, the majority of telomeric DNA is duplicated by conventional DNA replication. A fundamental step in eukaryotic DNA replication involves chromatin binding of the origin recognition complex (ORC). In human cells, telomeric repeat binding factor 2 (TRF2) is thought to play a role in the recruitment of ORC onto telomeres...
November 9, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27816684/role-of-telomeres-and-associated-maintenance-genes-in-type-2-diabetes-mellitus-a-review
#2
REVIEW
Itty Sethi, G R Bhat, Vinod Singh, Rakesh Kumar, A J S Bhanwer, Rameshwar N K Bamezai, Swarkar Sharma, Ekta Rai
Type 2 Diabetes Mellitus (T2DM), a multifactorial complex disorder, is emerging as a major cause of morbidity, mortality and socio-economic burden across the world. Despite huge efforts in understanding genetics of T2DM, only ∼10% of the genetic factors have been identified so far. Telomere attrition, a natural phenomenon has recently emerged in understanding the pathophysiology of T2DM. It has been indicated that Telomeres and associated pathways might be the critical components in the disease etiology, though the mechanism(s) involved are not clear...
October 21, 2016: Diabetes Research and Clinical Practice
https://www.readbyqxmd.com/read/27785368/ercc1-and-telomere-status-in-breast-tumours-treated-with-neoadjuvant-chemotherapy-and-their-association-with-patient-prognosis
#3
Mathilde Gay-Bellile, Pierre Romero, Anne Cayre, Lauren Véronèse, Maud Privat, Shalini Singh, Patricia Combes, Fabrice Kwiatkowski, Catherine Abrial, Yves-Jean Bignon, Philippe Vago, Frédérique Penault-Llorca, Andreï Tchirkov
Dysfunctional telomeres and DNA damage repair (DDR) play important roles in cancer progression. Studies have reported correlations between these factors and tumour aggressiveness and clinical outcome in breast cancer. We studied the characteristics of telomeres and expression of ERCC1, a protein involved in a number of DNA repair pathways and in telomere homeostasis, to assess their prognostic value, alone or in combination, in 90 residual breast tumours after treatment with neoadjuvant chemotherapy (NCT). ERCC1 status was investigated at different molecular levels (protein and gene expression and gene copy-number variations) by immunohistochemistry, qRT-PCR and quantitative multiplex fluorescent-PCR (QMF-PCR)...
October 2016: Journal of Pathology. Clinical Research
https://www.readbyqxmd.com/read/27771897/profiling-of-signaling-proteins-in-penumbra-after-focal-photothrombotic-infarct-in-the-rat-brain-cortex
#4
Svetlana Demyanenko, Anatoly Uzdensky
In ischemic stroke, cell damage propagates from infarct core to surrounding tissue. To reveal proteins involved in neurodegeneration and neuroprotection, we explored the protein profile in penumbra surrounding the photothrombotic infarct core induced in rat cerebral cortex by local laser irradiation after Bengal Rose administration. Using antibody microarrays, we studied changes in expression of 224 signaling proteins 1, 4, or 24 h after photothrombotic infarct compared with untreated contralateral cortex...
October 22, 2016: Molecular Neurobiology
https://www.readbyqxmd.com/read/27699234/telomere-dysfunction-in-alveolar-epithelial-cells-causes-lung-remodeling-and-fibrosis
#5
Ram P Naikawadi, Supparerk Disayabutr, Benat Mallavia, Matthew L Donne, Gary Green, Janet L La, Jason R Rock, Mark R Looney, Paul J Wolters
Telomeres are short in type II alveolar epithelial cells (AECs) of patients with idiopathic pulmonary fibrosis (IPF). Whether dysfunctional telomeres contribute directly to development of lung fibrosis remains unknown. The objective of this study was to investigate whether telomere dysfunction in type II AECs, mediated by deletion of the telomere shelterin protein TRF1, leads to pulmonary fibrosis in mice (SPC-Cre TRF1(fl/fl) mice). Deletion of TRF1 in type II AECs for 2 weeks increased γH2AX DNA damage foci, but not histopathologic changes in the lung...
September 8, 2016: JCI Insight
https://www.readbyqxmd.com/read/27612935/telomere-length-defines-the-cardiomyocyte-differentiation-potency-of-mouse-induced-pluripotent-stem-cells
#6
Tania Aguado, Francisco J Gutiérrez, Esther Aix, Ralph P Schneider, Giovanna Giovinnazo, María A Blasco, Ignacio Flores
Induced pluripotent stem cells (iPSCs) can be differentiated in vitro and in vivo to all cardiovascular lineages and are therefore a promising cell source for cardiac regenerative therapy. However, iPSC lines do not all differentiate into cardiomyocytes (CMs) with the same efficiency. Here, we show that telomerase-competent iPSCs with relatively long telomeres and high expression of the shelterin-complex protein TRF1 (iPSC(highT) ) differentiate sooner and more efficiently into CMs than those with relatively short telomeres and low TRF1 expression (iPSC(lowT) )...
September 10, 2016: Stem Cells
https://www.readbyqxmd.com/read/27563064/molecular-architecture-of-full-length-trf1-favors-its-interaction-with-dna
#7
Jasminka Boskovic, Jaime Martinez-Gago, Marinela Mendez-Pertuz, Alberto Buscato, Jorge Luis Martinez-Torrecuadrada, Maria A Blasco
Telomeres are specific DNA-protein structures found at both ends of eukaryotic chromosomes that protect the genome from degradation and from being recognized as double-stranded breaks. In vertebrates, telomeres are composed of tandem repeats of the TTAGGG sequence that are bound by a six-subunit complex called shelterin. Molecular mechanisms of telomere functions remain unknown in large part due to lack of structural data on shelterins, shelterin complex, and its interaction with the telomeric DNA repeats. TRF1 is one of the best studied shelterin components; however, the molecular architecture of the full-length protein remains unknown...
October 7, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27556185/pinx1-structure-regulation-and-its-functions-in-cancer
#8
Hai-Long Li, Jun Song, Hong-Mei Yong, Ping-Fu Hou, Yan-Su Chen, Wen-Bo Song, Jin Bai, Jun-Nian Zheng
PIN2/TRF1-interacting telomerase inhibitor 1 (PinX1) is a novel cloned gene located at human chromosome 8p23, playing a vital role in maintaining telomeres length and chromosome stability. It has been demonstrated to be involved in tumor genesis and progression in most malignancies. However, some researches showed opposing molecular status of PinX1 gene and its expression patterns in several other types of tumors. The pathogenic mechanism of PinX1 expression in human malignancy is not yet clear. Moreover, emerging evidence suggest that PinX1 (especially its TID domain) might be a potential new target cancer treatment...
August 19, 2016: Oncotarget
https://www.readbyqxmd.com/read/27500188/telomere-dysfunction-induced-foci-tif-analysis
#9
Ilgen Mender, Jerry W Shay
Telomerase maintains telomeric DNA in eukaryotes during early developments, ~90% of cancer cells and some proliferative stem like cells. Telomeric repeats at the end of chromosomes are associated with the shelterin complex. This complex consists of TRF1, TRF2, Rap1, TIN2, TPP1, POT1 which protect DNA from being recognized as DNA double-stranded breaks. Critically short telomeres or impaired shelterin proteins can cause telomere dysfunction, which eventually induces DNA damage responses at the telomeres. DNA damage responses can be identified by antibodies to 53BP1, gammaH2AX, Rad17, ATM, and Mre11...
November 20, 2015: Bio-protocol
https://www.readbyqxmd.com/read/27455024/-advance-in-research-on-the-function-of-telomeric-shelterin-component-tpp1-and-its-relationship-with-characteristics-of-tumors
#10
REVIEW
Lihua Yao, Xiaolan Guo
As an important telomere binding protein, TPP1 protects the ends of telomeres and maintains the stability and integrity of its structure and function by interacting with other five essential core proteins (POT1, TRF1, TRF2, TIN2, and RAP1) to form a complex called Shelterin. Recently, researchers have discovered that TPP1 participates in protection of telomeres and regulation of telomerase activity. The relationship between TPP1 and tumorigenesis, tumor progression and treatment has also been investigated. This paper reviews the latest findings of TPP1 regarding to its structure, function and interaction with other proteins involved in tumorigenesis...
August 2016: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/27450562/molecular-basis-and-quantitative-assessment-of-trf1-and-trf2-protein-interactions-with-tin2-and-apollo-peptides
#11
Umesh Kalathiya, Monikaben Padariya, Maciej Baginski
Shelterin is a six-protein complex (TRF1, TRF2, POT1, RAP1, TIN2, and TPP1) that also functions in smaller subsets in regulation and protection of human telomeres. Two closely related proteins, TRF1 and TRF2, make high-affinity contact directly with double-stranded telomeric DNA and serve as a molecular platform. Protein TIN2 binds to TRF1 and TRF2 dimer-forming domains, whereas Apollo makes interaction only with TRF2. To elucidate the molecular basis of these interactions, we employed molecular dynamics (MD) simulations of TRF1TRFH-TIN2TBM and TRF2TRFH-TIN2TBM/ApolloTBM complexes and of the isolated proteins...
July 22, 2016: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/27427384/fancd2-limits-blm-dependent-telomere-instability-in-the-alternative-lengthening-of-telomeres-pathway
#12
Heather Root, Andrew Larsen, Martin Komosa, Fakhriya Al'Azri, Ren Li, David P Bazett-Jones, M Stephen Meyn
Fanconi Anemia and Bloom syndrome are genomic instability syndromes caused by mutations in proteins that participate in overlapping DNA repair and replication pathways. Here, we show that the monoubiquitinated form of the Fanconi Anemia protein FANCD2 acts in opposition to the BLM DNA helicase to restrain telomere replication and recombination in human cells that utilize the Alternative Lengthening of Telomeres (ALT) pathway. ALT relies on exchanges of telomeric DNA to maintain telomeres, a process that we show FANCD2 suppresses...
July 17, 2016: Human Molecular Genetics
https://www.readbyqxmd.com/read/27425647/tankyrase-as-a-novel-molecular-target-in-cancer-and-fibrotic-diseases
#13
Vijaya Lakshmi Tiruveedi, Swarna Bale, Amit Khurana, Chandraiah Godugu
Tankyrases belong to a group of enzymes called poly ADP ribosyl polymerases (PARPs). With the advent of a new class of small molecule inhibitors of PARP for clinical use like OLAPARIB; that gained accelerated approval by the USFDA in treating ovarian and breast cancers, the horizons of the PARPs as a novel target in various disease conditions has risen. Tankyrases (PARP 5) are yet another class of PARPs that perform poly ADP ribosylation on different substrate proteins aiding in progression of many diseases like cancer, fibrosis, diabetes and neurological disorders even...
July 15, 2016: Current Drug Targets
https://www.readbyqxmd.com/read/27391261/optogenetic-regulation-of-site-specific-subtelomeric-dnamethylation
#14
Samrat Roy Choudhury, Yi Cui, Anoop Narayanan, David P Gilley, Nazmul Huda, Chiao-Ling Lo, Feng C Zhou, Dinesh Yernool, Joseph Irudayaraj
Telomere length homeostasis, critical for chromosomal integrity and genome stability, is controlled by intricate molecular regulatory machinery that includes epigenetic modifications. Here, we examine site-specific and spatiotemporal alteration of the subtelomeric methylation of CpG islands using optogenetic tools to understand the epigenetic regulatory mechanisms of telomere length maintenance. Human DNA methyltransferase3A (DNMT3A) were assembled selectively at chromosome ends by fusion to cryptochrome 2 protein (CRY2) and its interacting complement, the basic helix loop helix protein-1 (CIB1)...
July 4, 2016: Oncotarget
https://www.readbyqxmd.com/read/27367027/inflammatory-related-gene-ikk%C3%AE-ikk%C3%AE-ikk%C3%AE-cooperates-to-determine-liver-cancer-stem-cells-progression-by-altering-telomere-via-heterochromatin-protein-1-hotair-axis
#15
Jiahui An, Mengying Wu, Xiaoru Xin, Zhuojia Lin, Xiaonan Li, Qidi Zheng, Xin Gui, Tianming Li, Hu Pu, Haiyan Li, Dongdong Lu
Cancer stem cells are associated with tumor recurrence. IKK is a protein kinase that is composed of IKKα, IKKβ, IKKγ. Herein, we demonstrate that IKKα plus IKKβ promoted and IKKγ inhibited liver cancer stem cell growth in vitro and in vivo. Mechanistically, IKKα plus IKKβ enhanced and IKKγ inhibited the interplay among HP1α, HP1β and HP1γ that competes for the interaction among HP1α, SUZ12, HEZ2. Therefore, IKKα plus IKKβ inhibited and IKKγ enhanced the activity of H3K27 methyltransferase SUZ12 and EZH2, which methylates H3K27 immediately sites on HOTAIR promoter region...
June 29, 2016: Oncotarget
https://www.readbyqxmd.com/read/27353491/differential-gene-expression-in-mouse-spermatogonial-stem-cells-and-embryonic-stem-cells
#16
Yinshan Bai, Meiying Feng, Shanshan Liu, Hengxi Wei, Li Li, Xianwei Zhang, Chao Shen, Shouquan Zhang, Ningfang Ma
Mouse spermatogonial stem cells (mSSCs) may be reprogrammed to become pluripotent stem cells under in vitro culture conditions, due to epigenetic modifications, which are closely associated with the expression of transcription factors and epigenetic factors. Thus, this study was conducted to compare the gene expression of transcription factors and epigenetic factors in mSSCs and mouse embryonic stem cells (mESCs). Firstly, the freshly isolated mSSCs [mSSCs (f)] were enriched by magnetic-activated cell sorting with Thy1...
August 2016: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/27349711/rapamycin-safeguards-lymphocytes-from-dna-damage-accumulation-in-vivo
#17
Amel Chebel, Régine Catallo, Céline Mabon, Emmanuel Bachy, Thomas Wenner, Gilles Salles, Claire Pouteil-Noble, Martine Ffrench
Several studies reported the benefits of switching from anticalcineurins to mTOR inhibitors to avoid cancer occurrence after organ transplantation. The purpose of our study was to determine in vivo biological markers to explain these benefits. Cellular changes related to cellular senescence and DNA damage were analyzed in peripheral blood lymphocytes. Thirty-five kidney transplanted patients receiving anticalcineurins were investigated: 17 patients were proposed to switch to rapamycin and 18 patients with similar age and transplantation duration, continued anticalcineurins...
September 2016: European Journal of Cell Biology
https://www.readbyqxmd.com/read/27320927/rif1-regulates-the-fate-of-dna-entanglements-during-mitosis
#18
Sophie Zaaijer, Nadeem Shaikh, Rishi Kumar Nageshan, Julia Promisel Cooper
Clearance of entangled DNA from the anaphase mid-region must accurately proceed in order for chromosomes to segregate with high fidelity. Loss of Taz1 (fission yeast ortholog of human TRF1/TRF2) leads to stalled telomeric replication forks that trigger telomeric entanglements; the resolution of these entanglements fails at ≤20°C. Here, we investigate these entanglements and their promotion by the conserved replication/repair protein Rif1. Rif1 plays no role in taz1Δ fork stalling. Rather, Rif1 localizes to the anaphase mid-region and regulates the resolution of persisting DNA structures...
June 28, 2016: Cell Reports
https://www.readbyqxmd.com/read/27298259/functional-interplay-between-sa1-and-trf1-in-telomeric-dna-binding-and-dna-dna-pairing
#19
Jiangguo Lin, Preston Countryman, Haijiang Chen, Hai Pan, Yanlin Fan, Yunyun Jiang, Parminder Kaur, Wang Miao, Gisele Gurgel, Changjiang You, Jacob Piehler, Neil M Kad, Robert Riehn, Patricia L Opresko, Susan Smith, Yizhi Jane Tao, Hong Wang
Proper chromosome alignment and segregation during mitosis depend on cohesion between sister chromatids. Cohesion is thought to occur through the entrapment of DNA within the tripartite ring (Smc1, Smc3 and Rad21) with enforcement from a fourth subunit (SA1/SA2). Surprisingly, cohesin rings do not play a major role in sister telomere cohesion. Instead, this role is replaced by SA1 and telomere binding proteins (TRF1 and TIN2). Neither the DNA binding property of SA1 nor this unique telomere cohesion mechanism is understood...
July 27, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27257545/de-novo-assembly-and-characterization-of-the-chinese-three-keeled-pond-turtle-mauremys-reevesii-transcriptome-presence-of-longevity-related-genes
#20
Huazong Yin, Liuwang Nie, Feifei Zhao, Huaxing Zhou, Haifeng Li, Xianmei Dong, Huanhuan Zhang, Yuqin Wang, Qiong Shi, Jun Li
Mauremys reevesii (Geoemydidae) is one of the most common and widespread semi-aquatic turtles in East Asia. The unusually long lifespan of some individuals makes this turtle species a potentially useful model organism for studying the molecular basis of longevity. In this study, pooled total RNA extracted from liver, spleen and skeletal-muscle of three adult individuals were sequenced using Illumina Hiseq 2500 platform. A set of telomere-related genes were found in the transcriptome, including tert, tep1, and six shelterin complex proteins coding genes (trf1, trf2, tpp1, pot1, tin2 and rap1)...
2016: PeerJ
keyword
keyword
100931
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"