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MST1 AND Supplement

Bo Gao, Wang Sun, Xianqi Wang, Xu Jia, Biao Ma, Yu Chang, Weihui Zhang, Dongbo Xue
Bone marrow endothelial cells (BMECs) are important components of the hematopoietic microenvironment in bone marrow, and they can secrete several types of cytokines to regulate the functions of hematopoietic stem/progenitor cells. To date, it is unknown whether BMECs undergo functional changes and lead to hematopoietic abnormalities in cases of liver cirrhosis (LC). In the present study, whole genome microarray analysis was carried out to detect differentially expressed genes in human BMECs treated for 48 h with medium supplemented with 20% pooled sera from 26 patients with LC or 10 healthy volunteers as the control group...
November 2013: International Journal of Molecular Medicine
Sung-Hwa Sohn, Ki-Nam Kim, In Kyoung Kim, Eun-Il Lee, Jae-Jun Ryu, Meyoung-Kon Kim
The goal of this study was to determine the effects of tobacco compounds on gene expression in a human fetal lung cell line (WI38). In the present study, we investigated the effects of tobacco compounds (nicotine, benzo(a)pyrene (B(a)P), and 2-Naphthylamine) on gene expression profiles in human fetal fibroblasts using cDNA microarray and real-time PCR. WI38 cells were cultured in Eagle's minimum essential medium (MEM) supplemented with 10% fetal bovine serum, 2% 200 mM L-glutamine, and a 2% penicillin and streptomycin solution...
August 2008: Environmental Toxicology
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