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Alberto Tejero Rioseras, Kapil Dev Singh, Nora Nowak, Martin T Gaugg, Tobias Bruderer, Renato Zenobi, Pablo M-L Sinues
The tricarboxylic acid (TCA) cycle is one of the most important metabolic pathway for cellular respiration in aerobic organisms. It provides and collects intermediates for many other interconnecting pathways and acts as a hub connecting metabolism of carbohydrates, fatty acids, and amino acids. Alteration in intracellular levels of its intermediates has been linked with a wide range of illnesses ranging from cancer to cellular necrosis or liver cirrhosis. Therefore, there exists an intrinsic interest in monitoring such metabolites...
May 16, 2018: Analytical Chemistry
Dina S Salem, Mahmoud A Sliem, Mohamed El-Sesy, Samia A Shouman, Yehia Badr
A green method was used for producing gold nanoparticles (Au NPs) using chitosan as a natural cationic, biodegradable and biocompatible polymer. In this method, chitosan acts as a reducing and stabilizing agent for the synthesis of Au NPs. Different concentrations of chitosan solutions (0.01%, 0.05%, 0.1%, 0.2%, 0.5% and 1%) were applied. In an attempt to mitigate the side effects of anti-cancer drug, 5-fluorouracil (5-FU), through reducing drug doses in photothermal therapy, the formed positively-charged chitosan-wrapped Au NPs were used as a drug delivery system for negatively charged 5-FU to hepatocellular carcinoma cells (HepG2)...
April 4, 2018: Journal of Photochemistry and Photobiology. B, Biology
Thomas Gaisl, Lukas Bregy, Nina Stebler, Martin Thomas Gaugg, Tobias Bruderer, Diego García-Gómez, Alexander Moeller, Florian Singer, Esther Irene Schwarz, Christian Benden, Pablo M-L Sinues, Renato Zenobi, Malcolm Kohler
We aimed at defining profiles of volatile organic compounds in exhaled breath from patients with cystic fibrosis (CF) using a novel real-time mass spectrometry technique.
 In this prospective matched case-control study, 30 patients with CF, and 30 healthy control subjects were matched one-to-one according to age, gender, and smoking state. We performed exhaled breath analysis by untargeted secondary electrospray ionization-high resolution mass spectrometry (SESI-HRMS). 
 Patients with CF (mean age 26...
March 20, 2018: Journal of Breath Research
D Zamora, M Amo-Gonzalez, M Lanza, G Fernández de la Mora, J Fernández de la Mora
The feasibility of detecting explosives in the atmosphere at concentrations as low as 0.01 ppq hinges on the poorly known question of what interfering species exist at these or higher concentrations. To clarify the issue, hundreds of samples of ambient air, either clean or loaded with explosives (from lightly contaminated environments) have been collected in fiberglass/stainless steel filters coated with Tenax-GR, thermally desorbed at variable temperature, and ionized with Cl- via secondary electrospray (SESI)...
February 20, 2018: Analytical Chemistry
Alberto Tejero Rioseras, Diego Garcia Gomez, Birgitta E Ebert, Lars M Blank, Alfredo J Ibáñez, Pablo M-L Sinues
While yeast is one of the most studied organisms, its intricate biology remains to be fully mapped and understood. This is especially the case when it comes to capture rapid, in vivo fluctuations of metabolite levels. Secondary electrospray ionization-high resolution mass spectrometry SESI-HRMS is introduced here as a sensitive and noninvasive analytical technique for online monitoring of microbial metabolic activity. The power of this technique is exemplarily shown for baker's yeast fermentation, for which the time-resolved abundance of about 300 metabolites is demonstrated...
October 27, 2017: Scientific Reports
Yi-Feng Cheng, Wenling Jiao, Qingqing Li, Yu Zhang, Sesi Li, Dandan Li, Renchao Che
Two hierarchical ZnO micro/nano-materials have been prepared by a short-time (45min) hydrothermal reaction without any surfactants. The different morphologies have been characterized comprehensively by XRD, SEM, TEM and XPS technologies. The hierarchical micro- and nano-structures are respectively consisted by the subordinate nano-pieces and nano-hexagonal-rods. Under the illumination of the simulated solar light, the nano-piece-aggregate spends 40min to degrade the 98% of rhodamine 6G solution (10(-5)mol/L), whereas the nano-rod-aggregate only degrades the 78% of the solution in the same condition...
August 31, 2017: Journal of Colloid and Interface Science
Daniele B Vinholes, Sérgio L Bassanesi, Hilton de Castro Chaves Junior, Carlos Alberto Machado, Ione M F Melo, Flavio Danni Fuchs, Sandra Costa Fuchs
BACKGROUND: Exposure to risk factors for hypertension may be influenced by the characteristics of the workplace, where workers spend most of their daily time. OBJECTIVES: To evaluate the association between features of the companies, particularly the presence of facilities to provide meals, and of population characteristics and the prevalence of hypertension, taking into account individual risk factors for hypertension. MATERIAL AND METHODS: This multilevel analysis was based on a cross-sectional study with individual and company data from the SESI (Serviço Social da Indústria-Social Service of Industries) study and population-based data from the national census statistics...
August 21, 2017: BMJ Open
Milad Nazari, Alexandra A Malico, Måns Ekelöf, Sean Lund, Gavin J Williams, David C Muddiman
Terpenes are the largest class of natural products with a wide range of applications including use as pharmaceuticals, fragrances, flavorings, and agricultural products. Terpenes are biosynthesized by the condensation of a variable number of isoprene units resulting in linear polyisoprene diphosphate units, which can then be cyclized by terpene synthases into a range of complex structures. While these cyclic structures have immense diversity and potential in different applications, their direct analysis in biological buffer systems requires intensive sample preparation steps such as salt cleanup, extraction with organic solvents, and chromatographic separations...
January 2018: Analytical and Bioanalytical Chemistry
Rafael V Picon, Juvenal S Dias-da-Costa, Flavio D Fuchs, Maria Teresa A Olinto, Niteesh K Choudhry, Sandra C Fuchs
Knowing the usual clinical practice is relevant for evaluations in health care and economic policies of management of hypertension. This study aimed to describe the usual management of hypertension in the Brazilian primary healthcare system through a systematic review and meta-analysis. The search of population-based studies conducted in Brazil was undertaken using PubMed, EMBASE, and Brazilian databases. Eligible studies were those conducted in adults with hypertension (blood pressure (BP) ≥ 140/90 mmHg or using BP lowering drugs)...
2017: International Journal of Hypertension
Mario Amo-Gonzalez, Juan Fernandez de la Mora
The differential mobility analyzer (DMA) is a narrow-band linear ion mobility filter operating at atmospheric pressure. It combines in series with a quadrupole mass spectrometer (Q-MS) for mobility/mass analysis, greatly reducing chemical noise in selected ion monitoring. However, the large flow rate of drift gas (~1000 L/min) required by DMAs complicates the achievement of high gas purity. Additionally, the symmetry of the drying counterflow gas at the interface of many commercial MS instruments, is degraded by the lateral motion of the drift gas at the DMA entrance slit...
August 2017: Journal of the American Society for Mass Spectrometry
Vivian Carolina Salgueiro, Natalia Lopes Pontes Iorio, Marcelle Cristina Ferreira, Raiane Cardoso Chamon, Kátia Regina Netto Dos Santos
BACKGROUND: Staphylococcus epidermidis is an opportunistic pathogen involved in hospital-acquired infections, particularly in those related to medical devices. This study characterized 50 genetically unrelated S. epidermidis isolates from bloodstream infections (BSIs, n = 31) and nares (n = 19) of neonates in relation to staphylococcal chromosomal cassette mec (SCCmec) type, biofilm production and associated genes, and the arginine catabolic mobile elements (ACME), in order to detect virulence factors that could discriminate a potential invasiveness isolate or predict an increasing pathogenicity...
January 13, 2017: BMC Microbiology
César Barrios-Collado, Diego García-Gómez, Renato Zenobi, Guillermo Vidal-de-Miguel, Alfredo J Ibáñez, Pablo Martinez-Lozano Sinues
We have deployed an efficient secondary electrospray ionization source coupled to an Orbitrap mass analyzer (SESI-MS) to investigate the emissions of a Begonia semperflorens. We document how hundreds of species can be tracked with an unparalleled time resolution of 2 min during day-night cycles. To further illustrate the capabilities of this system for volatile organic compounds (VOCs) analysis, we subjected the plant to mechanical damage and monitored its response. As a result, ∼1200 VOCs were monitored displaying different kinetics...
February 16, 2016: Analytical Chemistry
Esther I Schwarz, Pablo Martinez-Lozano Sinues, Lukas Bregy, Thomas Gaisl, Diego Garcia Gomez, Martin T Gaugg, Yannick Suter, Nina Stebler, Yvonne Nussbaumer-Ochsner, Konrad E Bloch, John R Stradling, Renato Zenobi, Malcolm Kohler
BACKGROUND: Obstructive sleep apnoea (OSA) is highly prevalent and associated with cardiovascular and metabolic changes. OSA is usually diagnosed by polysomnography which is time-consuming and provides little information on the patient's phenotype thus limiting a personalised treatment approach. Exhaled breath contains information on metabolism which can be analysed by mass spectrometry within minutes. The objective of this study was to identify a breath profile in OSA recurrence by use of secondary-electrospray-ionization-mass spectrometry (SESI-MS)...
February 2016: Thorax
Sesi Ayodele Hotonu, Shoaib Khan, Richard Jeavons
Bilateral septic arthritis of the shoulder is uncommon in the immunocompetent patient with no previous risk factors for joint infection, and is thus easily missed. Septic arthritis is associated with significant rates of morbidity and mortality. Early diagnosis and management is the key to a favourable outcome; septic arthritis should be considered as a differential diagnosis in the unwell patient presenting with shoulder pain and reduced range of joint movement. We present a case of a 47-year-old previously fit and well man with bilateral shoulder septic arthritis...
November 20, 2015: BMJ Case Reports
Matthew J Aernecke, Ted Mendum, Geoff Geurtsen, Alla Ostrinskaya, Roderick R Kunz
A rapid method for vapor pressure measurement was developed and used to derive the vapor pressure curve of the thermally labile peroxide-based explosive hexamethylene triperoxide diamine (HMTD) over the temperature range from 28 to 80 °C. This method uses a controlled flow of vapor from a solid-phase HMTD source that is presented to an ambient-pressure-ionization mass spectrometer equipped with a secondary-electrospray-ionization (SESI) source. The subpart-per-trillion sensitivity of this system enables direct detection of HMTD vapor through an intact [M + H](+) ion in real time at temperatures near 20 °C...
November 25, 2015: Journal of Physical Chemistry. A
Lukas Bregy, Annick R Müggler, Pablo Martinez-Lozano Sinues, Diego García-Gómez, Yannick Suter, Georgios N Belibasakis, Malcolm Kohler, Patrick R Schmidlin, Renato Zenobi
The detection of bacterial-specific volatile metabolites may be a valuable tool to predict infection. Here we applied a real-time mass spectrometric technique to investigate differences in volatile metabolic profiles of oral bacteria that cause periodontitis. We coupled a secondary electrospray ionization (SESI) source to a commercial high-resolution mass spectrometer to interrogate the headspace from bacterial cultures and human saliva. We identified 120 potential markers characteristic for periodontal pathogens Aggregatibacter actinomycetemcomitans (n = 13), Porphyromonas gingivalis (n = 70), Tanerella forsythia (n = 30) and Treponema denticola (n = 7) in in vitro cultures...
2015: Scientific Reports
Anna Długosz, Ewa Niedźwiedzka, Tomasz Długosz, Lidia Wądołowska
INTRODUCTION: Under-nutrition, over-nutrition and obesity incidence in relation to environmental diversity and socio-economic influences in adolescents from less urbanized regions of Poland has not been widely studied. OBJECTIVE: To determine the correlation between socio-economic status and incidence of underweight, overweight and obesity in adolescents located in less-urbanized regions of Poland. MATERIAL AND METHODS: The study involved 553 adolescents aged 13-18 living in 2 less-urbanized regions of Poland (small towns and villages in the central and north-eastern regions)...
2015: Annals of Agricultural and Environmental Medicine: AAEM
Pablo Martinez-Lozano Sinues, Elena Landoni, Rosalba Miceli, Vincenza F Dibari, Matteo Dugo, Roberto Agresti, Elda Tagliabue, Simone Cristoni, Rosaria Orlandi
Breath analysis represents a new frontier in medical diagnosis and a powerful tool for cancer biomarker discovery due to the recent development of analytical platforms for the detection and identification of human exhaled volatile compounds. Statistical and bioinformatic tools may represent an effective complement to the technical and instrumental enhancements needed to fully exploit clinical applications of breath analysis. Our exploratory study in a cohort of 14 breast cancer patients and 11 healthy volunteers used secondary electrospray ionization-mass spectrometry (SESI-MS) to detect a cancer-related volatile profile...
September 2015: Journal of Breath Research
Joanna Kowalkowska, Lidia Wadolowska, Justyna Weronika Wuenstel, Małgorzata Anna Słowińska, Ewa Niedźwiedzka
BACKGROUND: The aim of this study was to analyze the association between overweight prevalence and socioeconomic status (SES) measured by complex SES index and single SES factors in Polish adolescents in respect to age and sex. METHODS: This cross-sectional study was conducted in 2010-2011. A total of 1,176 adolescents aged 13.0-18.9 years were included. The respondents were students of junior-high and high schools from northern, eastern and central Poland. Quota sampling by sex and age was used...
July 2014: Iranian Journal of Public Health
Heather D Bean, Theodore R Mellors, Jiangjiang Zhu, Jane E Hill
A number of direct injection mass spectrometry methods that can sample foods nondestructively and without sample preparation are being developed with applications ranging from the rapid assessment of food safety to the verification of protected designations of origin. In this pilot study, secondary electrospray ionization mass spectrometry (SESI-MS) in positive- and negative-ion modes was used to collect volatile fingerprints of artisanal Cheddar cheeses aged for one to three years. SESI-MS fingerprints were found to change in an aging-dependent manner and can be used to descriptively and predictively categorize Cheddars by their aging period, identify volatile components that increase or decrease with aging, and robustly discriminate individual batches of artisanal cheese...
May 6, 2015: Journal of Agricultural and Food Chemistry
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