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Vivian Carolina Salgueiro, Natalia Lopes Pontes Iorio, Marcelle Cristina Ferreira, Raiane Cardoso Chamon, Kátia Regina Netto Dos Santos
BACKGROUND: Staphylococcus epidermidis is an opportunistic pathogen involved in hospital-acquired infections, particularly in those related to medical devices. This study characterized 50 genetically unrelated S. epidermidis isolates from bloodstream infections (BSIs, n = 31) and nares (n = 19) of neonates in relation to staphylococcal chromosomal cassette mec (SCCmec) type, biofilm production and associated genes, and the arginine catabolic mobile elements (ACME), in order to detect virulence factors that could discriminate a potential invasiveness isolate or predict an increasing pathogenicity...
January 13, 2017: BMC Microbiology
César Barrios-Collado, Diego García-Gómez, Renato Zenobi, Guillermo Vidal-de-Miguel, Alfredo J Ibáñez, Pablo Martinez-Lozano Sinues
We have deployed an efficient secondary electrospray ionization source coupled to an Orbitrap mass analyzer (SESI-MS) to investigate the emissions of a Begonia semperflorens. We document how hundreds of species can be tracked with an unparalleled time resolution of 2 min during day-night cycles. To further illustrate the capabilities of this system for volatile organic compounds (VOCs) analysis, we subjected the plant to mechanical damage and monitored its response. As a result, ∼1200 VOCs were monitored displaying different kinetics...
February 16, 2016: Analytical Chemistry
Esther I Schwarz, Pablo Martinez-Lozano Sinues, Lukas Bregy, Thomas Gaisl, Diego Garcia Gomez, Martin T Gaugg, Yannick Suter, Nina Stebler, Yvonne Nussbaumer-Ochsner, Konrad E Bloch, John R Stradling, Renato Zenobi, Malcolm Kohler
BACKGROUND: Obstructive sleep apnoea (OSA) is highly prevalent and associated with cardiovascular and metabolic changes. OSA is usually diagnosed by polysomnography which is time-consuming and provides little information on the patient's phenotype thus limiting a personalised treatment approach. Exhaled breath contains information on metabolism which can be analysed by mass spectrometry within minutes. The objective of this study was to identify a breath profile in OSA recurrence by use of secondary-electrospray-ionization-mass spectrometry (SESI-MS)...
February 2016: Thorax
Sesi Ayodele Hotonu, Shoaib Khan, Richard Jeavons
Bilateral septic arthritis of the shoulder is uncommon in the immunocompetent patient with no previous risk factors for joint infection, and is thus easily missed. Septic arthritis is associated with significant rates of morbidity and mortality. Early diagnosis and management is the key to a favourable outcome; septic arthritis should be considered as a differential diagnosis in the unwell patient presenting with shoulder pain and reduced range of joint movement. We present a case of a 47-year-old previously fit and well man with bilateral shoulder septic arthritis...
2015: BMJ Case Reports
Matthew J Aernecke, Ted Mendum, Geoff Geurtsen, Alla Ostrinskaya, Roderick R Kunz
A rapid method for vapor pressure measurement was developed and used to derive the vapor pressure curve of the thermally labile peroxide-based explosive hexamethylene triperoxide diamine (HMTD) over the temperature range from 28 to 80 °C. This method uses a controlled flow of vapor from a solid-phase HMTD source that is presented to an ambient-pressure-ionization mass spectrometer equipped with a secondary-electrospray-ionization (SESI) source. The subpart-per-trillion sensitivity of this system enables direct detection of HMTD vapor through an intact [M + H](+) ion in real time at temperatures near 20 °C...
November 25, 2015: Journal of Physical Chemistry. A
Lukas Bregy, Annick R Müggler, Pablo Martinez-Lozano Sinues, Diego García-Gómez, Yannick Suter, Georgios N Belibasakis, Malcolm Kohler, Patrick R Schmidlin, Renato Zenobi
The detection of bacterial-specific volatile metabolites may be a valuable tool to predict infection. Here we applied a real-time mass spectrometric technique to investigate differences in volatile metabolic profiles of oral bacteria that cause periodontitis. We coupled a secondary electrospray ionization (SESI) source to a commercial high-resolution mass spectrometer to interrogate the headspace from bacterial cultures and human saliva. We identified 120 potential markers characteristic for periodontal pathogens Aggregatibacter actinomycetemcomitans (n = 13), Porphyromonas gingivalis (n = 70), Tanerella forsythia (n = 30) and Treponema denticola (n = 7) in in vitro cultures...
2015: Scientific Reports
Anna Długosz, Ewa Niedźwiedzka, Tomasz Długosz, Lidia Wądołowska
INTRODUCTION: Under-nutrition, over-nutrition and obesity incidence in relation to environmental diversity and socio-economic influences in adolescents from less urbanized regions of Poland has not been widely studied. OBJECTIVE: To determine the correlation between socio-economic status and incidence of underweight, overweight and obesity in adolescents located in less-urbanized regions of Poland. MATERIAL AND METHODS: The study involved 553 adolescents aged 13-18 living in 2 less-urbanized regions of Poland (small towns and villages in the central and north-eastern regions)...
2015: Annals of Agricultural and Environmental Medicine: AAEM
Pablo Martinez-Lozano Sinues, Elena Landoni, Rosalba Miceli, Vincenza F Dibari, Matteo Dugo, Roberto Agresti, Elda Tagliabue, Simone Cristoni, Rosaria Orlandi
Breath analysis represents a new frontier in medical diagnosis and a powerful tool for cancer biomarker discovery due to the recent development of analytical platforms for the detection and identification of human exhaled volatile compounds. Statistical and bioinformatic tools may represent an effective complement to the technical and instrumental enhancements needed to fully exploit clinical applications of breath analysis. Our exploratory study in a cohort of 14 breast cancer patients and 11 healthy volunteers used secondary electrospray ionization-mass spectrometry (SESI-MS) to detect a cancer-related volatile profile...
September 2015: Journal of Breath Research
Joanna Kowalkowska, Lidia Wadolowska, Justyna Weronika Wuenstel, Małgorzata Anna Słowińska, Ewa Niedźwiedzka
BACKGROUND: The aim of this study was to analyze the association between overweight prevalence and socioeconomic status (SES) measured by complex SES index and single SES factors in Polish adolescents in respect to age and sex. METHODS: This cross-sectional study was conducted in 2010-2011. A total of 1,176 adolescents aged 13.0-18.9 years were included. The respondents were students of junior-high and high schools from northern, eastern and central Poland. Quota sampling by sex and age was used...
July 2014: Iranian Journal of Public Health
Heather D Bean, Theodore R Mellors, Jiangjiang Zhu, Jane E Hill
A number of direct injection mass spectrometry methods that can sample foods nondestructively and without sample preparation are being developed with applications ranging from the rapid assessment of food safety to the verification of protected designations of origin. In this pilot study, secondary electrospray ionization mass spectrometry (SESI-MS) in positive- and negative-ion modes was used to collect volatile fingerprints of artisanal Cheddar cheeses aged for one to three years. SESI-MS fingerprints were found to change in an aging-dependent manner and can be used to descriptively and predictively categorize Cheddars by their aging period, identify volatile components that increase or decrease with aging, and robustly discriminate individual batches of artisanal cheese...
May 6, 2015: Journal of Agricultural and Food Chemistry
Antje Spijker-Huiges, Karin Vermeulen, Jan C Winters, Marten van Wijhe, Klaas van der Meer
OBJECTIVE: To investigate the effect of adding segmental epidural steroid injections (SESIs) to usual care compared with usual care alone on quality of life and cost utility in lumbosacral radicular syndrome (LRS) in general practice. DESIGN: A pragmatic randomized controlled trial. Results were analyzed using mixed models. SETTING: Primary care. PARTICIPANTS: Patients (N=50) in the acute phase of LRS. INTERVENTIONS: One epidural injection containing 80mg of triamcinolone in normal saline...
March 2015: Archives of Physical Medicine and Rehabilitation
Jan-Christoph Wolf, Martin Schaer, Peter Siegenthaler, Renato Zenobi
A novel active capillary dielectric barrier discharge plasma ionization (DBDI) technique for mass spectrometry is applied to the direct detection of 13 chemical warfare related compounds, including sarin, and compared to secondary electrospray ionization (SESI) in terms of selectivity and sensitivity. The investigated compounds include an intact chemical warfare agent and structurally related molecules, hydrolysis products and/or precursors of highly toxic nerve agents (G-series, V-series, and "new" nerve agents), and blistering and incapacitating warfare agents...
January 6, 2015: Analytical Chemistry
Heather D Bean, Jaime Jiménez-Díaz, Jiangjiang Zhu, Jane E Hill
In this model study, we explored the host's contribution of breath volatiles to diagnostic secondary electrospray ionisation-mass spectrometry (SESI-MS) breathprints for acute bacterial lung infections, their correlation with the host's immune response, and their use in identifying the lung pathogen. Murine airways were exposed to Pseudomonas aeruginosa and Staphylococcus aureus bacterial cell lysates or to PBS (controls), and their breath and bronchoalveolar lavage fluid (BALF) were collected at six time points (from 6 to 120 h) after exposure...
January 2015: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
Heather D Bean, Jiangjiang Zhu, Jackson C Sengle, Jane E Hill
Invasive methicillin-resistant Staphylococcus aureus (MRSA) infections are a serious health threat, causing an estimated 11,000 deaths per year in the United States. MRSA pneumonias account for 16% of invasive infections, and can be difficult to detect as the current state-of-the-art diagnostics require that bacterial DNA is recovered from the infection site. Because 60% of patients with invasive infections die within 7 d of culturing positive for MRSA, earlier detection of the pathogen may significantly reduce mortality...
December 2014: Journal of Breath Research
Antje Spijker-Huiges, Jan C Winters, Marten van Wijhe, Klaas Groenier
BACKGROUND: Lumbosacral radicular syndrome (LRS) is a self-limiting, benign, painful and impairing condition caused by lumbar disc herniation and inflammatory processes around the nerve root. Segmental epidural steroid injections (SESIs) are helpful to reduce radicular pain on a short-term basis. It is unknown whether SESIs are an effective addition to usual pain treatment of LRS in general practice. In our study, we assessed the effectiveness of SESIs on pain and disability as an addition to usual care for acute LRS in general practice...
2014: BMC Musculoskeletal Disorders
James C Reynolds, Modupe A Jimoh, Cristina Guallar-Hoyas, Colin S Creaser, Salman Siddiqui, C L Paul Thomas
A two-stage thermal desorption/secondary electrospray ionization/time-of-flight mass spectrometry for faster targeted breath profiling has been studied. A new secondary electrospray ionization (SESI) source was devised to constrain the thermal desorption plume and promote efficient mixing in the ionization region. Further, a chromatographic pre-separation stage was introduced to suppress interferences from siloxanes associated with thermal desorption profiles of exhaled breath samples.In vitro tests with 5-nonanone indicated an increased sensitivity and a lowered limit-of-detection, both by a factor of ~4, the latter to an on-trap mass of 14...
September 2014: Journal of Breath Research
Anqiang Zhang, Wenwen Deng, Yaling Lin, Junhui Ye, Yaomin Dong, Yufeng Lei, Hongtao Chen
A novel supramolecular elastomer (SESi) based on multiple hydrogen bond associations between low-molecular-weight polydimethylsiloxane chains was obtained through a two-step reaction of linear carboxyl-terminated polydimethylsiloxane oligomers with diethylenetriamine and urea, and the reaction mechanism was characterized. The results of differential scanning calorimetry and X-ray diffraction analyses indicated that the supramolecular network structure is completely amorphous, endowing SESi with rubber-like elastic behavior at room temperature...
2014: Journal of Biomaterials Science. Polymer Edition
Helen J Martin, James C Reynolds, Svetlana Riazanskaia, C L Paul Thomas
The non-invasive nature of volatile organic compound (VOC) sampling from skin makes this a priority in the development of new screening and diagnostic assays. Evaluation of recent literature highlights the tension between the analytical utility of ambient ionisation approaches for skin profiling and the practicality of undertaking larger campaigns (higher statistical power), or undertaking research in remote locations. This study describes how VOC may be sampled from skin and recovered from a polydimethylsilicone sampling coupon and analysed by thermal desorption (TD) interfaced to secondary electrospray ionisation (SESI) time-of-flight mass spectrometry (MS) for the high throughput screening of volatile fatty acids (VFAs) from human skin...
September 7, 2014: Analyst
You-Fei Zheng, Jin-Xin Cheng, Rong-Jun Wu, Fu-Lai Guan, Shu-Ran Yao
From the viewpoint of land surface evapotranspiration, and by using the semi-empirical evapotranspiration model based on the Priestley-Taylor equation and the land surface temperature-vegetation index (LST-VI) triangle algorithm, the current monitoring technology of agricultural drought based on remote sensing was improved, and a simplified Evapotranspiration Stress Index (SESI) was derived. With the application of the MODIS land products from March to November in 2008 and 2009, the triangle algorithm modeling with three different schemes was constructed to calculate the SESI to monitor the agricultural drought in the plain areas of Beijing, Tianjin, and Hebei, in comparison with the Temperature Vegetation Dryness Index (TVDI)...
September 2013: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
Achilleas Sesis, Mark Hodnett, Gianluca Memoli, Andrew J Wain, Izabela Jurewicz, Alan B Dalton, J David Carey, Gareth Hinds
Ultrasonication is the most widely used technique for the dispersion of a range of nanomaterials, but the intrinsic mechanism which leads to stable solutions is poorly understood with procedures quoted in the literature typically specifying only extrinsic parameters such as nominal electrical input power and exposure time. Here we present new insights into the dispersion mechanism of a representative nanomaterial, single-walled carbon nanotubes (SW-CNTs), using a novel up-scalable sonoreactor and an in situ technique for the measurement of acoustic cavitation activity during ultrasonication...
December 5, 2013: Journal of Physical Chemistry. B
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