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https://www.readbyqxmd.com/read/29622864/comparison-of-immunohistochemistry-cytochemistry-and-flow-cytometry-in-aml-for-myeloperoxidase-detection
#1
Ankur Ahuja, Seema Tyagi, Tulika Seth, Hara Prasad Pati, Gps Gahlot, Preeti Tripathi, Venkatesan Somasundaram, Renu Saxena
Acute Myeloid Leukemia (AML) as per World Health Organization (WHO 2008) classification is on the basis of the antigenic characterization, enzymes restriction in the neoplastic myeloid cells and the specific translocations/mutations. AML can be assessed and differentiated by flowcytometry (FCM)/immunohistochemistry (IHC)/cytochemistry techniques. Myeloperoxidase (MPO) is an unequivocal marker to differentiate AML from the acute lymphoblastic leukemia. Despite FCM popularity, it has its limitations, in form of 'dry-tap', cost, and inability of being performed by retrospective analysis...
April 2018: Indian Journal of Hematology & Blood Transfusion
https://www.readbyqxmd.com/read/29607996/-unidirectionally-oriented-membrane-formation-of-supra-polysaccharides-sacran-and-application-to-drug-delivery-system
#2
Kosuke Okeyoshi, Maiko K Okajima, Tatsuo Kaneko
 The geometric structures of soft materials can be controlled on the macro-scale using interfacial or mechanical instability, e.g., fingering patterns of viscous liquid and buckling patterns of gels during swelling/deswelling. These patterns can be used as smart materials for capturing/releasing and mass-transportation applications. Here we introduce the emergence of a uniaxially oriented membrane by drying an aqueous liquid-crystalline solution, composed of megamolecular supra-polysaccharides "sacran", from a limited space...
2018: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
https://www.readbyqxmd.com/read/29605998/humidity-responsive-gold-aerogel-for-real-time-monitoring-of-human-breath
#3
Israt Ali, Liming Chen, Youju Huang, Liping Song, Xuefei Lu, Baoqing Liu, Lei Zhang, Jiawei Zhang, Linxi Hou, Tao Chen
Humidity sensor has received considerable attention in recent years because of its significance and wide applications in agriculture, industries, goods stores and medical fields. However, the conventional humidity sensors usually possessed the complexed sensing mechanism, low sensitivity, and required a time-consuming, labor-intensive process. The exploring an ideal sensing material to amplify the sensitivity of humidity sensor is still a big challenge. Herein, we developed a simple, low-cost and scalable fabrication strategy to construct a highly sensitive humidity sensor based on polymer/gold nanoparticles (AuNPs) hybrid materials...
April 1, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29602568/evaluation-of-ophthalmic-surgical-instrument-sterility-using-short-cycle-sterilization-for-sequential-same-day-use
#4
David F Chang, Nikki Hurley, Nick Mamalis, Jeffrey Whitman
PURPOSE: The common practice of short-cycle sterilization for ophthalmic surgical instrumentation has come under increased regulatory scrutiny. This study was undertaken to evaluate the efficacy of short-cycle sterilization processing for consecutive same-day cataract procedures. DESIGN: Testing of specific sterilization processing methods by an independent medical device validation testing laboratory. PARTICIPANTS: Phaco handpieces from 3 separate manufacturers were tested along with appropriate biologic indicators and controls using 2 common steam sterilizers...
March 27, 2018: Ophthalmology
https://www.readbyqxmd.com/read/29600796/rapid-screening-for-klinefelter-syndrome-with-a-simple-high-resolution-melting-assay-a-multicenter-study
#5
Dong-Mei Fu, Yu-Lin Zhou, Jing Zhao, Ping Hu, Zheng-Feng Xu, Shi-Ming Lv, Jun-Jie Hu, Zhong-Min Xia, Qi-Wei Guo
Klinefelter syndrome (KS) is the set of symptoms that result from the presence of an extra X chromosome in males. Postnatal population-based KS screening will enable timely diagnosis of this common chromosomal disease, providing the opportunity for early intervention and therapy at the time point when they are most effective and may prevent later symptoms or complications. Therefore, through this study, we introduced a simple high-resolution melting (HRM) assay for KS screening and evaluated its clinical sensitivity and specificity in three medical centers using 1373 clinical blood samples...
March 30, 2018: Asian Journal of Andrology
https://www.readbyqxmd.com/read/29587350/polymeric-nanoparticles-for-increasing-oral-bioavailability-of-curcumin
#6
Anita Umerska, Caroline Gaucher, Felipe Oyarzun-Ampuero, Isabelle Fries-Raeth, Florence Colin, María Gabriela Villamizar-Sarmiento, Philippe Maincent, Anne Sapin-Minet
Despite the promising biological and antioxidant properties of curcumin, its medical applications are limited due to poor solubility in water and low bioavailability. Polymeric nanoparticles (NPs) adapted to oral delivery may overcome these drawbacks. Properties such as particle size, zeta potential, morphology and encapsulation efficiency were assessed. Then, the possibility of storing these NPs in a solid-state form obtained by freeze-drying, in vitro curcumin dissolution and cytocompatibility towards intestinal cells were evaluated...
March 24, 2018: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/29582965/-screening-test-of-fabry-disease-in-patients-with-renal-replacement-therapy-in-the-city-of-modena
#7
Gaetano Alfano, Nicola Ganda, Caterina Cerami, Giacomo Mori, Francesco Fontana, Gianni Cappelli
Background: Fabry disease is a rare genetic lysosomal storage disease, inherited in an X-linked manner, characterized by lysosomal deposition of globotriaosylceramide due to deficient activity of the enzyme α-galactosidase A. Because the prevalence of this genetic disorder is unknown in the Emilia Romagna region, we conducted a screening study to assess the prevalence of Fabry disease in the city of Modena, Italy. Material and Methods: A screening study has been conducted in patients on renal replacement therapy at University Hospital of Modena...
March 2018: Giornale Italiano di Nefrologia: Organo Ufficiale Della Società Italiana di Nefrologia
https://www.readbyqxmd.com/read/29560966/medical-cannabis-in-the-treatment-of-cancer-pain-and-spastic-conditions-and-options-of-drug-delivery-in-clinical-practice
#8
Leos Landa, Jan Jurica, Jiri Sliva, Monika Pechackova, Regina Demlova
The use of cannabis for medical purposes has been recently legalised in many countries including the Czech Republic. As a result, there is increased interest on the part of physicians and patients in many aspects of its application. This mini review briefly covers the main active substances of the cannabis plant and mechanisms of action. It focuses on two conditions, cancer pain and spasticity in multiple sclerosis, where its effects are well-documented. A comprehensive overview of a few cannabis-based products and the basic pharmacokinetics of marijuana's constituents follows...
March 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/29552839/-sulfur-dioxide-limit-standard-and-residues-in-chinese-medicinal-materials
#9
Chuan-Zhi Kang, Wan-Zhen Yang, Ge Mo, Li Zhou, Jing-Yi Jiang, Chao-Geng Lv, Sheng Wang, Tao Zhou, Ye Yang, Lan-Ping Guo
The traditional sulfur fumigation processing method has been widely used in the initial processing and storage of traditional Chinese medicinal materials due to its economy, efficiency, convenience, high operability and effect on mold and insect prevention. However, excessive sulfur fumigation of traditional Chinese medicinal materials would lead to the changes in chemical compositions, and even endanger human health. This study showed that traditional Chinese medicinal materials were sulfur fumigated directly after being harvested for quick drying, or fumigated after being weted in the storage process for preventing mold and insects...
January 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29521240/brassica-nigra-ethopharmacological-review-of-a-routinely-used-condiment
#10
Surendra S Agrawal, Tapasya P Yallatikar, Pravina N Gurjar
Brassica nigra belonging to the family Brassicaceae (syn - black mustard) comprises of dried seeds and is widely cultivated in Mediterranean region and various other countries like India and Europe. B. nigra contains glycosinolates which undergoes hydrolysis in the presence of myrosinase enzyme yields allyl isothiocyante. The bitter taste and pungent odor is due to the isothiocyanates. B. Nigra shows various activities that are medically important such as anticancer, anti-bacterial, anti-fungal, anti-helminthic, protection against renal and hepatic toxicity and can also be used in diabetes treatment...
March 8, 2018: Current Drug Discovery Technologies
https://www.readbyqxmd.com/read/29502521/beneficial-effects-of-a-novel-shark-skin-collagen-dressing-for-the-promotion-of-seawater-immersion-wound-healing
#11
Xian-Rong Shen, Xiu-Li Chen, Hai-Xia Xie, Ying He, Wei Chen, Qun Luo, Wei-Hong Yuan, Xue Tang, Deng-Yong Hou, Ding-Wen Jiang, Qing-Rong Wang
BACKGROUND: Wounded personnel who work at sea often encounter a plethora of difficulties. The most important of these difficulties is seawater immersion. Common medical dressings have little effect when the affected area is immersed in seawater, and only rarely dressings have been reported for the treatment of seawater-immersed wounds. The objective of this study is to develop a new dressing which should be suitable to prevent the wound from seawater immersion and to promote the wound healing...
October 27, 2017: Military Medical Research
https://www.readbyqxmd.com/read/29476846/optimizing-duodenoscope-reprocessing-rigorous-assessment-of-a-culture-and-quarantine-protocol
#12
Jennifer T Higa, Jaehoon Choe, Deborah Tombs, Michael Gluck, Andrew S Ross
BACKGROUND AND AIMS: Iatrogenic infections related to duodenoscopes remain a top concern for medical centers where ERCP is performed. We assessed the long-term results and impact of key interventions in the optimization of a rigorous "culture and quarantine" program for duodenoscope reprocessing. METHODS: We reviewed a prospectively collected, quality assurance database of all duodenoscope cultures (n = 4307) obtained for the initial 3-year duration of culture and quarantine from 2014 to 2017 in a single U...
February 21, 2018: Gastrointestinal Endoscopy
https://www.readbyqxmd.com/read/29470090/engineering-interfacial-processes-at-mini-micro-nano-scales-using-sessile-droplet-architecture
#13
Lalit Bansal, Apratim Sanyal, Prasenjit Kabi, Binita Pathak, Saptarshi Basu
Evaporating sessile functional droplets act as the fundamental building block that control the cumulative outcome of many industrial and biological applications such as surface patterning, 3D printing, photonic crystals, DNA sequencing, to name a few. Additionally, a drying single sessile droplet forms a high throughput processing technique using low material volume which is especially suitable for medical diagnosis. Sessile droplet also provides an elementary platform to study and analyze fundamental interfacial processes at various lengthscale ranging from macroscopically observable wetting and evaporation to microfluidic transport to interparticle forces operating at a nanometric lengthscale...
February 22, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29447949/protective-effects-of-alpinae-oxyphyllae-fructus-extracts-on-lipopolysaccharide-induced-animal-model-of-alzheimer-s-disease
#14
Yunlong Wang, Mengshi Wang, Kaiyue Fan, Tongde Li, Tingxu Yan, Bo Wu, Kaishun Bi, Ying Jia
ETHNOPHARMACOLOGICAL RELEVANCE: Alpinae Oxyphyllae Fructus (AOF) with warming and tonifying the kidney and spleen, anti-salivation, anti-polyuria and anti-diarrhea functions is the dried ripe fruits of Alpinia oxyphylla Miq. (Zingiberaceae). As a traditional Chinese medicine, its application history is very long. AIMS OF THE STUDY: The purpose of our study is to investigate the effects of different solvent extracts from AOF on lipopolysaccharide (LPS)-induced animal model of Alzheimer's disease (AD) to elucidate the traditional medical theories with modern pharmacological methods and provide a reference for further clarifying its active components and mechanisms...
February 12, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29446772/production-of-ready-to-use-few-layer-graphene-in-aqueous-suspensions
#15
Jose M González-Domínguez, Verónica León, María Isabel Lucío, Maurizio Prato, Ester Vázquez
Graphene has promising physical and chemical properties such as high strength and flexibility, coupled with high electrical and thermal conductivities. It is therefore being incorporated into polymer-based composites for use in electronics and photonics applications. A main constraint related to the graphene development is that, being of a strongly hydrophobic nature, almost all dispersions (usually required for its handling and processing toward the desired application) are prepared in poisonous organic solvents such as N-methyl pyrrolidone or N,N-dimethyl formamide...
March 2018: Nature Protocols
https://www.readbyqxmd.com/read/29438752/design-and-evaluation-of-chitosan-poly-l-lactide-pectin-based-composite-scaffolds-for-cartilage-tissue-regeneration
#16
Sarada Prasanna Mallick, Bhisham Narayan Singh, Amit Rastogi, Pradeep Srivastava
Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential. Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM)...
February 10, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29438634/regulating-cannabis-manufacturing-applying-public-health-best-practices-from-tobacco-control
#17
Daniel G Orenstein, Stanton A Glantz
State legalization and regulation of cannabis, despite continued federal illegality, is a massive shift in regulatory approach. Manufactured cannabis, including concentrates, extracts, edibles, tinctures, topicals and other products, has received less attention than more commonly used dried flower, but represents emerging regulatory challenges due to additives, potency, consumption methods, and abuse and misuse potential. In November 2017, the California Department of Public Health (CDPH) released initial cannabis manufacturing regulations as part of a new state regulatory structure...
February 13, 2018: Journal of Psychoactive Drugs
https://www.readbyqxmd.com/read/29433308/aqueous-zinc-compounds-as-residual-antimicrobial-agents-for-textiles
#18
Brandon Holt, Shawn Gregory, Todd A Sulchek, Shannon Yee, Mark D Losego
Textiles, especially those worn by patients and medical professionals, serve as a vector for proliferating pathogens. Upstream manufacturing techniques and end user practices, such as transition metal embedment in textile fibers or alcohol-based disinfectants, can mitigate pathogen growth, but both techniques have their shortcomings. Fiber embedment requires complete replacement of all fabrics in a facility, and the effects of embedded nanoparticles on human health remain unknown. Alcohol-based, end-user disinfectants are short-lived because they quickly volatilize...
February 13, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29389582/tea-silkworm-droppings-as-an-enriched-source-of%C3%A2-tea-flavonoids
#19
Tzu-Yun Chou, Meei-Ju Yang, Shih-Kung Tseng, Shoei-Sheng Lee, Chia-Chuan Chang
Andraca droppings is the waste excreted from the tea biter Andraca theae. Its chemical constituents and potential medical use, unlike those of the traditional Chinese medicine silkworm droppings, have not been reported yet. To explore new nutraceuticals, the chemical constituents of this substance were investigated. Since the bioactive ingredients are generally present in the EtOAc-soluble fraction, this fraction, obtained from the ethanolic extract of the dried Andraca droppings by liquid-liquid partitioning, was separated by chromatographic methods, including Sephadex LH-20, centrifugal partition chromatography, and RP-18 columns, to produce 14 compounds (1-14)...
January 2018: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/29377638/biofluid-spectroscopic-disease-diagnostics-a-review-on-the-processes-and-spectral-impact-of-drying
#20
REVIEW
James M Cameron, Holly J Butler, David S Palmer, Matthew J Baker
The complex patterns observed from evaporated liquid drops have been examined extensively over the last 20 years. Complete understanding of drop deposition is vital in many medical processes, and one which is essential to the translation of biofluid spectroscopic disease diagnostics. The promising use of spectroscopy in disease diagnosis has been hindered by the complicated patterns left by dried biological fluids which may inhibit the clinical translation of this technology. Coffee-ring formation, cracking and gelation patterns have all been observed in biofluid drops, and with surface homogeneity being a key element to many spectroscopic techniques, experimental issues have been found to arise...
January 26, 2018: Journal of Biophotonics
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