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aging yeast

Stephen K Jones, Eric C Spivey, James R Rybarski, Ilya J Finkelstein
Whole-lifespan single-cell analysis has greatly increased our understanding of fundamental cellular processes such as cellular aging. To observe individual cells across their entire lifespan, all progeny must be removed from the growth medium, typically via manual microdissection. However, manual microdissection is laborious, low-throughput, and incompatible with fluorescence microscopy. Here, we describe assembly and operation of the multiplexed-Fission Yeast Lifespan Microdissector (multFYLM), a high-throughput microfluidic device for rapidly acquiring single-cell whole-lifespan imaging...
April 5, 2018: Bio-protocol
Kalliopi Kostelidou, Ilias Matis, Georgios Skretas
Neurodegenerative diseases (ND) are a major threat to the aging population and the lack of a single preventive or disease-modifying agent only serves to increase their impact. In the past few years, protein misfolding and the subsequent formation of neurotoxic oligomeric/aggregated protein species have emerged as a unifying theme underlying the pathology of these complex diseases. Recently developed microbial genetic screens and selection systems for monitoring ND-associated protein misfolding have allowed the establishment of high-throughput assays for the identification of cellular factors and processes that are important mediators of ND-associated proteotoxicities...
May 15, 2018: Current Pharmaceutical Design
Eun Jin Lee, Jiye Hyun, Yong-Ho Choi, Byung-Serk Hurh, Sang-Ho Choi, Inhyung Lee
Doenjang (Korean fermented soybean paste) with an improved flavor and safety was prepared by the simultaneous fermentation of autochthonous mixed starters at the pilot plan scale. First, whole soybean meju was fermented by coculturing safety-verified starters Aspergillus oryzae MJS14 and Bacillus amyloliquefaciens zip6 or Bacillus subtilis D119C. These fermented whole soybean meju were aged in a brine solution after the additional inoculation of Tetragenococcus halophilus 7BDE22 and Zygosaccharomyces rouxii SMY045 to yield doenjang...
May 15, 2018: Journal of Food Science
J P F Santos, A A Aquino, M B A Glória, M J Avila-Campos, P M Oba, K de M Santos, T H A Vendramini, A C Carciofi, A R Junior, M A Brunetto
This study evaluated the effects of increasing concentrations of spray-dried yeast cell wall (YCW) in diets for healthy adult cats on apparent nutrient digestibility and on bacterial composition and fermentation products in the stool. Fourteen cats with an average weight of 4.40 ± 1.05 kg and an average age of 6.2 ± 0.54 years were used and assigned to treatments in an unbalanced randomized block design (by experimental period) with two blocks and three or four cats per diet in each block. Treatments included: control (0% YCW), 0...
May 14, 2018: Journal of Animal Physiology and Animal Nutrition
Juan Manuel Del Fresno, Iris Loira, Antonio Morata, Carmen González, Jose Antonio Suárez-Lepe, Rafael Cuerda
Ageing on lees (AOL) is a technique that increases volatile compounds, promotes colour stability, improves mouthfeel and reduces astringency in red wines. The main drawback is that it is a slow process. Several months are necessary to obtain perceptible effects in wines. Different authors have studied the application of new techniques to accelerate the AOL process. Ultrasound (US) has been used to improve different food industry processes; it could be interesting to accelerate the yeast autolysis during AOL...
September 30, 2018: Food Chemistry
Silvana Paredes, Maria Angulo-Ibanez, Luisa Tasselli, Scott M Carlson, Wei Zheng, Tie-Mei Li, Katrin F Chua
In the yeast Saccharomyces cerevisiae, genomic instability in rDNA repeat sequences is an underlying cause of cell aging and is suppressed by the chromatin-silencing factor Sir2. In humans, rDNA instability is observed in cancers and premature aging syndromes, but its underlying mechanisms and functional consequences remain unclear. Here, we uncovered a pivotal role of sirtuin 7 (SIRT7), a mammalian Sir2 homolog, in guarding against rDNA instability and show that this function of SIRT7 protects against senescence in primary human cells...
May 4, 2018: Journal of Biological Chemistry
Diana Macedo, Carolina Jardim, Inês Figueira, A Filipa Almeida, Gordon J McDougall, Derek Stewart, Jose E Yuste, Francisco A Tomás-Barberán, Sandra Tenreiro, Tiago F Outeiro, Cláudia N Santos
Parkinson's disease (PD) is an age-related neurodegenerative disease associated with the misfolding and aggregation of alpha-synuclein (aSyn). The molecular underpinnings of PD are still obscure, but nutrition may play an important role in the prevention, onset, and disease progression. Dietary (poly)phenols revert and prevent age-related cognitive decline and neurodegeneration in model systems. However, only limited attempts were made to evaluate the impact of digestion on the bioactivities of (poly)phenols and determine their mechanisms of action...
May 3, 2018: Scientific Reports
Song-Quan Ong, Zairi Jaal
Larval age and nutrition significantly affected the insect's physiology. These influences are important when rearing a population of vectors that is used to monitor the resistance level, in which standardized conditions are crucial for a more harmonized result. Little information has been reported on the effects of larval age and nutrition on the susceptibility of insects to insecticides, and therefore, we studied the effects on the susceptibility of Culex quinquefasciatus Say's (Diptera: Culicidae) larvae to temephos by comparing the median lethal concentration (LC50) after 24 hr between the second and fourth instar larvae and between the larvae that fed on protein-based and carbohydrate-based larval diets...
March 1, 2018: Journal of Insect Science
Libuše Váchová, Zdena Palková
Yeasts, like other microorganisms, create numerous types of multicellular communities, which differ in their complexity, cell differentiation and in the occupation of different niches. Some of the communities, such as colonies and some types of biofilms, develop by division and subsequent differentiation of cells growing on semisolid or solid surfaces to which they are attached or which they can penetrate. Aggregation of individual cells is important for formation of other community types, such as multicellular flocs, which sediment to the bottom or float to the surface of liquid cultures forming flor biofilms, organized at the border between liquid and air under specific circumstances...
June 1, 2018: FEMS Yeast Research
Kunal Baxi, Carlos E de Carvalho
The nematode Caenorhabditis elegans (C. elegans) is a model system that is widely used to study longevity and developmental pathways. Such studies are facilitated by the transparency of the animal, the ability to do forward and reverse genetic assays, the relative ease of generating fluorescently labeled proteins, and the use of fluorescent dyes that can either be microinjected into the early embryo or incorporated into its food (E. coli strain OP50) to label cellular organelles (e.g. 9-diethylamino-5H-benzo(a)phenoxazine-5-one and (3-{2-[(1H,1'H-2,2'-bipyrrol-5-yl-kappaN(1))methylidene]-2H-pyrrol-5-yl-kappaN}-N-[2-(dimethylamino)ethyl]propanamidato)(difluoro)boron)...
April 13, 2018: Journal of Visualized Experiments: JoVE
Mahnaz Fatahinia, Sima Jafarpour, Abdollah Rafiei, Simin Taghipour, Koichi Makimura, Ali Rezaei-Matehkolaei
Background and Purpose: Onychomycosis is fungal infection of the nails with an overall increasing incidence, worldwide. The epidemiological aspects of onychomycosis in Khuzestan, Iran, have not been established. In this study, we aimed to evaluate the clinical and mycological status of fungal nail infection in Khuzestan Province, Iran. Materials and Methods: The study population included 433 patients (143 males vs. 290 females). Nail samples underwent primary direct microscopy and culture...
December 2017: Advances in Medical Mycology (Iran)
Mohammad T Ismail, Abeer Al-Kafri, Mazen Ismail
Background and Purpose: Otomycosis is a fungal infection that frequently involves the external auditory canal. The epidemiologic data on the etiologic agents of otomycosis in Syria are very limited. In this study, we aimed to determine the fungal agents, gender distribution, and clinical presentation of otomycosis. Materials and Methods: Two hundred and ninety nine patients (153 [51.17%] male and 146 [48.83%] female) clinically prediagnosed as otomycosis were studied at Al-mouassat University Hospital and ENT Crescent Syrian Clinic...
September 2017: Advances in Medical Mycology (Iran)
Emily M Ross, Patrick H Maxwell
A variety of mild stresses have been shown to extend lifespan in diverse species through hormesis, which is a beneficial response to a stress or toxin that would cause a negative response at a higher exposure. Whether particular stresses induce hormesis can vary with genotype for a given species, and the underlying mechanisms of lifespan extension are only partly understood in most cases. We show that low doses of the DNA damaging or replication stress agents hydroxyurea, methyl methanesulfonate, 4-nitroquinoline 1-oxide, or Zeocin (a phleomycin derivative) lengthened chronological lifespan in Saccharomyces cerevisiae if cells were exposed during growth, but not if they were exposed during stationary phase...
April 26, 2018: Experimental Gerontology
Joanna Klim, Arkadiusz Gładki, Roza Kucharczyk, Urszula Zielenkiewicz, Szymon Kaczanowski
Apoptotic cell death is a type of eukaryotic cell death. In animals, it regulates development, is involved in cancer suppression, and causes cell death during pathological aging of neuronal cells in neurodegenerative diseases such as Alzheimer's. Mitochondrial apoptotic-like cell death, a form of primordial apoptosis, also occurs in unicellular organisms. Here, we ask the question why the apoptosis machinery has been acquired and maintained in unicellular organisms and attempt to answer it by performing ancestral state reconstruction...
April 27, 2018: G3: Genes—Genomes—Genetics
Anindita Sen, Deblina Bhunia, Pijush Kanti Datta, Atanu Ray, Parthajit Banerjee
Background: Onychomycosis (OM) is a major public health problem which is increasing worldwide. It is associated with high morbidity and causes physical, psychological, and occupational problems in patients. Aims: This study aims to study the pattern of etiological agents, clinical features, and severity assessment of OM in this part of India. Materials and Methods: Sixty eight clinically suspected patients with positive potassium hydroxide and fungal culture were studied...
March 2018: Indian Journal of Dermatology
Hanbo Li, Baofeng Su, Guyu Qin, Zhi Ye, Ahmed Elaswad, Ahmed Alsaqufi, Dayan A Perera, Zhenkui Qin, Ramji Odin, Khoi Vo, David Drescher, Dalton Robinson, Sheng Dong, Dan Zhang, Mei Shang, Nermeen Abass, Sanjay K Das, Max Bangs, Rex A Dunham
Repressible knockdown approaches were investigated to manipulate for transgenic sterilization in channel catfish, Ictalurus punctatus. Two primordial germ cell (PGC) marker genes, nanos and dead end, were targeted for knockdown and an off-target gene, vasa, was monitored. Two potentially copper-sensitive repressible promoters, yeast ctr3 (M) and ctr3-reduced (Mctr), were coupled with four knockdown strategies separately including: ds-sh RNA targeting the 5' end (N1) or 3' end (N2) of channel catfish nanos, full-length cDNA sequence of channel catfish nanos for overexpression (cDNA), and ds-sh RNA-targeting channel catfish dead end (DND)...
April 20, 2018: Marine Biotechnology
Sarah Schillie, Aaron Harris, Ruth Link-Gelles, José Romero, John Ward, Noele Nelson
Hepatitis B (HepB) vaccination is the primary means of preventing infections and complications caused by hepatitis B virus (HBV). On February 21, 2018, the Advisory Committee on Immunization Practices (ACIP) recommended Heplisav-B (HepB-CpG), a yeast-derived vaccine prepared with a novel adjuvant, administered as a 2-dose series (0, 1 month) for use in persons aged ≥18 years. The ACIP Hepatitis Vaccines Work Group conducted a systematic review of the evidence, including data from four randomized controlled trials assessing prevention of HBV infection and six randomized controlled trials assessing adverse events in adults...
April 20, 2018: MMWR. Morbidity and Mortality Weekly Report
Ning Liu, Jinquan Wang, Zhaoyang Liu, Yongchao Wang, Jianping Wang
This study aimed to investigate the effect of supplemental yeast cell walls (YCW) on growth performance, gut mucosal glutathione pathway, proteolytic enzymes, and transporters in broiler chickens from 1 to 21 d of age. One-day-old broiler chickens (n = 480) were randomly allocated to 4 treatments with 6 replicates of 20 chicks each for diets containing YCW at 0, 0.5, 1.0, and 1.5 g/kg. The results showed that YCW supplementation increased (P < 0.05) ADFI and ADG by 15.3% and 16.0%, respectively, and the 2 higher doses of YCW had greater effects (P < 0...
April 14, 2018: Journal of Animal Science
Anthony Arlia-Ciommo, Anna Leonov, Adam Beach, Vincent R Richard, Simon D Bourque, Michelle T Burstein, Pavlo Kyryakov, Alejandra Gomez-Perez, Olivia Koupaki, Rachel Feldman, Vladimir I Titorenko
A dietary regimen of caloric restriction delays aging in evolutionarily distant eukaryotes, including the budding yeast Saccharomyces cerevisiae . Here, we assessed how caloric restriction influences morphological, biochemical and cell biological properties of chronologically aging yeast advancing through different stages of the aging process. Our findings revealed that this low-calorie diet slows yeast chronological aging by mechanisms that coordinate the spatiotemporal dynamics of various cellular processes before entry into a non-proliferative state and after such entry...
March 23, 2018: Oncotarget
António Martins, Thorsten Pfirrmann, Stijn Heessen, Gustav Sundqvist, Vincent Bulone, Claes Andréasson, Per O Ljungdahl
Ssy5 is a signaling endoprotease that plays a key role in regulating central metabolism, cellular aging, and morphological transitions important for growth and survival of yeast (Saccharomyces cerevisiae) cells. In response to extracellular amino acids, Ssy5 proteolytically activates the transcription factors Stp1 and Stp2, leading to enhanced Ssy1-Ptr3-Ssy5 (SPS3) sensor-regulated gene expression. Ssy5 comprises a catalytic (Cat) domain and an extensive regulatory prodomain. However, Ssy5 is refractory to both broad-spectrum and serine protease-specific inhibitors, confounding its classification as a serine protease, and no information about Ssy5's cleavage-site preferences and its mechanism of substrate selection is available...
April 16, 2018: Journal of Biological Chemistry
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