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Teresa Graziano, Angela Amoruso, Stefania Nicola, Francesca Deidda, Serena Allesina, Marco Pane, Pietro Piffanelli, Francesco Strozzi, Luca Mogna, Mario Del Piano
GOALS: The aim of the study was to unequivocally demonstrate the nontransmissibility of the genes mediating the resistance of the strain Bifidobacterium longum W11 (LMG P-21586) to rifaximin. BACKGROUND: Most antibiotic treatments can induce unfavorable side effects such as antibiotic-associated diarrhea, which is largely attributable to the disruption of the intestinal microbiota. The parallel intake of probiotic bacteria might reduce these events, even if with generally very poor results...
November 2016: Journal of Clinical Gastroenterology
Hunny Sharma, G Y Yunus, Rohit Agrawal, Monika Kalra, Swati Verma, Supriya Bhattar
INTRODUCTION: From ancient times, plants with medicinal values are being tested and used in the treatment of various infectious diseases. AIMS AND OBJECTIVES: The present in vitro study was designed to assess the antifungal activity of three commonly available medicinal plants Glycyrrhiza glabra, Ficus religiosa, and Plantago major on inhibiting oral Candida albicans in comparison to standard antifungal agents. MATERIALS AND METHODS: Bark of G...
July 2016: Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research
Maryam Haghighatpanah, Amir Sasan Mozaffari Nejad, Ali Mojtahedi, Nour Amirmozafari, Habib Zeighami
Escherichia coli is an important cause of hospital-acquired infections worldwide. Antimicrobial resistance leads to treatment failure of hospital infections caused by E. coli. Production of extended-spectrum β-lactamases (ESBLs) is one of the major causes of antibiotic resistance in these bacteria. This study aimed to investigate the frequency of blaTEM and blaCTX-M genes in ESBL-producing E. coli strains isolated from clinical specimens of patients admitted to six hospitals in the north of Iran. A total of 160 E...
October 1, 2016: Journal of Global Antimicrobial Resistance
Hang Li, Pranav Balan, Akos Vertes
Metabolism in microbial colonies responds to competing species, rapidly evolving genetic makeup, and sometimes dramatic environmental changes. Conventional characterization of the existing and emerging microbial strains and their interactions with antimicrobial agents, e.g., the Kirby-Bauer susceptibility test, relies on time consuming methods with limited ability to discern the molecular mechanism and the minimum inhibitory concentration. Assessing the metabolic adaptation of microbial colonies requires their non-targeted molecular imaging in a native environment...
October 4, 2016: Angewandte Chemie
Alejandro Caicedo-Ramirez, Alison L Ling, Mark Hernandez
A new generation of laminates and cementitious materials incorporate antimicrobial metals into domestic infrastructure. Conventional culturing approaches are unsuitable for assessing the inhibitory properties of these materials. Modifications to the radial Kirby-Bauer antibiotic assay, which incorporate metal impregnated activated carbon in linear formats, reveal relative metal sensitivities of destructive acidophiles.
October 1, 2016: Journal of Microbiological Methods
Vincenzo Papa, Anna Rita Blanco, Marcello Santocono
PURPOSE: To characterize the ocular flora in a consecutive group of patients having cataract surgery and to determine the antibiotic susceptibility profile of isolates to several ophthalmic antibiotics. SETTING: Hospital Di Stefano, Catania, Italy. DESIGN: Observational case series. METHODS: Conjunctival and eyelid cultures from patients were obtained 14 days before surgery and, if positive, repeated the day of the surgery...
September 2016: Journal of Cataract and Refractive Surgery
Abid Hussain, Muhammad Sohail, Zaigham Abbas
OBJECTIVE: To determine the prevalence of Enterococcus faecalis and recent trends in antimicrobial sensitivity profiling. METHODS: The study was conducted at Chughtais Lahore Lab, Lahore, Pakistan, from December 2013 to May 2014, and comprised urine specimens from suspected patients. Antimicrobial profiling of isolated strains of Enterococcus faecalis was determined by Kirby-Bauer disc-diffusion method. RESULTS: Of the230 specimens, 161(70%) were positive for Enterococcus faecalis...
October 2016: JPMA. the Journal of the Pakistan Medical Association
Mehdi Goudarzi, Maryam Fazeli, Hossein Goudarzi, Mehdi Azad, Sima Sadat Seyedjavadi
BACKGROUND: The incidence of nosocomial Staphylococcus aureus infection is increasing annually and becoming a true global challenge. The pattern of Staphylococcus aureus protein A (spa) types in different geographic regions is diverse. OBJECTIVES: This study determined the prevalence of methicillin-resistant S. aureus and different spa types in S. aureus clinical isolates. MATERIALS AND METHODS: During a six-month period, 90 S. aureus isolates were recovered from 320 clinical specimens...
July 2016: Jundishapur Journal of Microbiology
Rolf Nyah-Tuku Nzalie, Hortense Kamga Gonsu, Sinata Koulla-Shiro
Introduction. Community-acquired urinary tract infections (CAUTIs) are usually treated empirically. Geographical variations in etiologic agents and their antibiotic sensitivity patterns are common. Knowledge of antibiotic resistance trends is important for improving evidence-based recommendations for empirical treatment of UTIs. Our aim was to determine the major bacterial etiologies of CAUTIs and their antibiotic resistance patterns in a cosmopolitan area of Cameroon for comparison with prescription practices of local physicians...
2016: International Journal of Microbiology
Nigus Zegeye, Daniel Asrat, Yimtubezinash Woldeamanuel, Abebe Habte, Etsegenet Gedlu, Tone Tønjum, Abraham Aseffa
BACKGROUND: Among children diagnosed to have chronic rheumatic valvular heart disease (RHD) in Ethiopia, many have been observed to develop recurrence of rheumatic fever (RF) despite secondary prophylaxis. This study determined the throat culture positivity rate and drug susceptibility pattern of beta hemolytic streptococci (BHS) isolated from children attending a specialized cardiac clinic in Ethiopia. METHODS: Throat swabs were collected from 233 children receiving benzathine penicillin injection as secondary prophylaxis for RHD and cultured...
September 23, 2016: BMC Infectious Diseases
Y L Gong, Z C Yang, S P Yin, M X Liu, C Zhang, X Q Luo, Y Z Peng
Objective: To analyze the distribution and drug resistance of pathogen isolated from severely burned patients with bloodstream infection, so as to provide reference for the clinical treatment of these patients. Methods: Blood samples of 162 severely burned patients (including 120 patients with extremely severe burn) with bloodstream infection admitted into our burn ICU from January 2011 to December 2014 were collected. Pathogens were cultured by fully automatic blood culture system, and API bacteria identification panels were used to identify pathogen...
September 20, 2016: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
Shamshul Ansari, Rabindra Dhital, Sony Shrestha, Sangita Thapa, Ram Puri, Niraj Chaudhary, Suresh Khatiwada, Rajendra Gautam
Introduction. Pseudomonas aeruginosa is the most frequently isolated organism as it acts as the opportunistic pathogen and can cause infections in immunosuppressed patients. The production of different types of beta-lactamases renders this organism resistant to many commonly used antimicrobials. Therefore, the aim of this study was to document the antibiotic resistance rate in Pseudomonas aeruginosa isolated from different clinical specimens. Methods. Pseudomonas aeruginosa recovered was identified by standard microbiological methods...
2016: BioMed Research International
Seada Ali, Tsegaye Sewunet, Zewdineh Sahlemariam, Gebre Kibru
BACKGROUND: Neisseria gonorrhoeae is a bacterium responsible for one of the classic sexually transmitted infection (STI) gonorrhea. Antibiotic resistant strains are emerging at alarming rate. Multiple sexual partners, unsafe sex and substance use habits are the main host related risk factors for acquiring the infection. Thus, this study aimed at determining the magnitude, its determinants and antimicrobial resistance profile of N. gonorrhoeae in a place where there is risk related cultural practices and relatively high HIV prevalence...
2016: BMC Research Notes
Richa Misra, Ritesh Thakare, N Amrin, Kashi Nath Prasad, Sidharth Chopra, Tapan Nirodhechand Dhole
BACKGROUND: We describe the antimicrobial susceptibility pattern of 100 typhoidal Salmonella isolates recovered from blood cultures and also investigate the association of decreased ciprofloxacin susceptibility with mutations in the genes coding for DNA gyrase and topoisomerase IV in 55 isolates. METHODS: The study was conducted between January 2013 and December 2015 at a tertiary care centre in north India. Antimicrobial susceptibility testing was performed by Kirby-Bauer disc diffusion and E-test...
August 2016: Transactions of the Royal Society of Tropical Medicine and Hygiene
Moise C Ngwa, Thomas Masalla, Seraphine Esemu, Foche Francis Fumoloh, Ian Kracalik, Eleonora Cella, Meer Taifur Alam, Jane-Francis Akoachere, Song Liang, Marco Salemi, J Glenn Morris, Afsar Ali, Lucy M Ndip
INTRODUCTION: During the cholera outbreak from 2010 to 2011 in Cameroon, 33,192 cases with 1,440 deaths (case fatality ratio 4.34%) were reported to the World Health Organization. Of these, the South West Region reported 3,120 clinical cases. This region is in the Equatorial Monsoon climatic subzone of Cameroon, close to the coast, raising questions as to whether cases were linked with development of environmental reservoirs. METHODS: In an investigation conducted by the Laboratory for Emerging Infectious Diseases, University of Buea, toxigenic V...
2016: PLoS Currents
S Roy, M A Hossain, S K Paul, N Haque, T K Barman, S Ahmed, S A Nasreen, M S Hossain, F Ahmed, P Biswas, F Nahar, H Begum, M S Islam
The aim of this study was to detect antimicrobial susceptibilities and the presence of drug resistance genes of MRSA from tertiary care hospitals. This study was carried out in the Department of Microbiology, Mymensingh Medical College during the period from Jan, 2015 to Dec, 2015. Clinical samples, including wound swab, pus, exudates from diabetic ulcer and burn ulcer, aural swab, blood and urine were collected. Standard microbiological procedure & biochemical tests were carried out to detect S. aureus. Oxacillin disk diffusion test was done by Kirby-Bauer disk diffusion method...
July 2016: Mymensingh Medical Journal: MMJ
Behrooz Heydari, Hossein Khalili, Iman Karimzadeh, Hamid Emadi-Kochak
In this study demographic, clinical, paraclinical, microbiological, and therapeutic features of patients with community-acquired acute bacterial meningitis admitted to a referral center for infectious diseases in Iran, have been evaluated. Medical records of adult (> 18 years) individuals with confirmed diagnosis of community-acquired bacterial meningitis during a 4-year period were retrospectively reviewed. All required data were obtained from patients' medical charts. Available findings about antimicrobial susceptibility of isolated bacteria from CSF and/or blood were also collected...
2016: Iranian Journal of Pharmaceutical Research: IJPR
Changsheng Kong, Jun Chen, Xiaoyan Zou, Yi Zhang, Lingna Zhu
OBJECTIVE: To determine the drug resistance of Comamonas testosteroni (C. testosteroni) by the Kirby-Bauer (K-B) method without Clinical and Laboratory Standards Institute (CLSI) explanation or the minimum inhibitory concentration (MIC) method with the standard CLSI explanation to evaluate the sensitivity of K-B method in detection of C. testosteroni.
 METHODS: K-B method and MIC method was used to determine the sensitivity of C. testosteroni to Piperacillin, Cefepime, Piperacillin/tazobactam, Imipenem, Meropenem, Amikacin, Gentamicin, Tobramycin, Ceftazidime and Ciprofloxacin...
August 2016: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
Nova Nayarit-Ballesteros, María Salud Rubio-Lozano, Enrique Delgado-Suárez, Danilo Méndez-Medina, Diego Braña-Varela, Oscar Rodas-Suárez
OBJECTIVE: To determine the serotype and antibiotic resistance profile of Salmonella spp. isolated from retail ground beef in Mexico City. MATERIALS AND METHODS: A total of 100 samples of ground beef were analyzed. The pathogen was isolated by conventional methods and confirmed by PCR (invA gene, 284 bp).The antibiotic resistance profile was determined by the Kirby-Bauer method while serotyping was performed according to the Kauffman-White scheme. RESULTS: We isolated a total of 19 strains of Lomita (6), Derby (4), Senftenberg (2), Javiana and Cannsttat (1) and undetermined (5) serotypes...
June 2016: Salud Pública de México
Shahram Shahraki-Zahedani, Shahnaz Rigi, Mohammad Bokaeian, Alireza Ansari-Moghaddam, Mehdi Moghadampour
INTRODUCTION: Extended-spectrum beta-lactamases (ESBLs) are bacterial enzymes capable of hydrolyzing beta-lactams. The aim of this study was to describe the prevalence of TEM- and SHV-type ESBL-producing Klebsiella pneumoniae strains in Zahedan, Southeast Iran. METHODS: A total of 170 non-repetitive K. pneumoniae strains were collected from patients referred to three teaching hospitals of Zahedan. Antibiotic susceptibility testing was determined for 17 antibiotics using the Kirby-Bauer disc diffusion method...
July 2016: Revista da Sociedade Brasileira de Medicina Tropical
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