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Carla D McArdle, Katie M Lagan, David A McDowell
BACKGROUND: This study investigated the resistance of bacteria isolated from diabetic foot ulcers (DFUs) to antibiotics frequently used in the management of the diabetic foot infections, at a range of pH values (pH 6.5, 7.5, and 8.5) known to exist in DFU wound fluid. This study aimed to determine whether changes (or atypical stasis) in wound fluid pH modulate the antibiotic resistance of DFU isolates, with potential implications in relation to the suppression/eradication of bacterial infections in DFUs...
January 2018: Journal of the American Podiatric Medical Association
Angeliki Kourmouli, Marco Valenti, Erwin van Rijn, Hubertus J E Beaumont, Olga-Ioanna Kalantzi, Andreas Schmidt-Ott, George Biskos
The use of disc diffusion susceptibility tests to determine the antibacterial activity of engineered nanoparticles (ENPs) is questionable because their low diffusivity practically prevents them from penetrating through the culture media. In this study, we investigate the ability of such a test, namely the Kirby-Bauer disc diffusion test, to determine the antimicrobial activity of Au and Ag ENPs having diameters from 10 to 40 nm on Escherichia coli cultures. As anticipated, the tests did not show any antibacterial effects of Au nanoparticles (NPs) as a result of their negligible diffusivity through the culture media...
2018: Journal of Nanoparticle Research: An Interdisciplinary Forum for Nanoscale Science and Technology
Qun Li, Hong Wang, Yanmei Xu, Xiangning Bai, Jianping Wang, Zhengdong Zhang, Xiang Liu, Yimao Miao, Ling Zhang, Xinqiong Li, Nianli Zou, Guodong Yan, Xi Chen, Jie Zhang, Shanshan Fu, Ruyue Fan, Jianguo Xu, Juan Li, Yanwen Xiong
Escherichia albertii is an emerging member of the Enterobacteriaceae causing human and animal enteric infections. Antimicrobial resistance among enteropathogens has been reported to be increasing in the past years. The purpose of this study was to investigate antibiotic resistance and resistance genes in E. albertii isolated from Zigong city, Sichuan province, China. The susceptibility to 21 antimicrobial agents was determined by Kirby-Bauer disk diffusion method. The highest prevalence was tetracycline resistance with a rate of 62...
2018: Frontiers in Microbiology
Robert F Potter, Meghan A Wallace, Allison R McMullen, Jerome Prusa, Christina L Stallings, Carey-Ann D Burnham, Gautam Dantas
OBJECTIVES: A carbapenem resistant Providencia rettgeri (PR1) isolate was recovered from a wound infection in Missouri, USA. This isolate possessed an EDTA inhibitable carbapenemase that was unidentified using the Xpert CARBA-R assay. Our objective was to elucidate the molecular determinant of carbapenem resistance in this isolate. We then sought to test the transmissibility of blaIMP-27 loci in clinical P. rettgeri and Proteus mirabilis isolates. METHODS: In October 2016 the novel ambler Class B carbapenemase blaIMP-27 , was reported in two different Proteus mirablis (PM185 and PM187) isolates...
February 26, 2018: Clinical Microbiology and Infection
S W Zuo, Z L Ni, Y B Yao, R S Yang, S K Wang, Y H Zhou
Objective: To investigate the antimicrobial susceptibility of Brucella and to provide a scientific basis for rational drug use and effective treatment of patients with brucellosis. Methods: A total of 41 Brucella strains were isolated from the blood of patients with brucellosis in 5 counties and 2 districts in Yuxi City, China from 2014 to 2016. The susceptibility to 23 antimicrobial drugs was tested using Kirby-Bauer (K-B) disk diffusion method and the sizes of antimicrobial rings were recorded. The susceptibility testing results were interpreted according to the Drug Susceptibility Testing Guideline (2009 version) ...
December 20, 2017: Chinese Journal of Industrial Hygiene and Occupational Diseases
Ronald P Kittle, Karla J McDermid, Lisa Muehlstein, George H Balazs
In Hawaii, glyphosate-based herbicides frequently sprayed near shorelines may be affecting non-target marine species. Glyphosate inhibits aromatic amino acid biosynthesis (shikimate pathway), and is toxic to beneficial gut bacteria in cattle and chickens. Effects of glyphosate on gut bacteria in marine herbivorous turtles were assessed in vitro. When cultures of mixed bacterial communities from gastrointestinal tracts of freshly euthanized green turtles (Chelonia mydas), were exposed for 24h to six glyphosate concentrations (plus deionized water control), bacterial density was significantly lower at glyphosate concentrations≥2...
February 2018: Marine Pollution Bulletin
Moisés Cipriano-Salazar, Saúl Rojas-Hernández, Jaime Olivares-Pérez, Régulo Jiménez-Guillén, Cruz-Lagunas Blas, Luis Miguel Camacho Díaz, Amadike Eziuche Ugbogu
This present study was conducted to evaluate the antimicrobial effects of tannic acid (TA) against isolated ruminal bacteria from adult sheep. Rumen samples were collected from two (2) adults sheep, and a total of nine (9) ruminal bacterial were isolated from the sample. The sensitivity of the ruminal bacteria isolates to 0.63, 1.25, 2.50, 5.00 and 10.00 mg TA/L was determined using clearance zone (CZ) of Kirby-Bauer disc diffusion susceptibility test. There was observable increase in the sensitivity of all bacterial isolates as the level of TA increases...
February 19, 2018: Microbial Pathogenesis
Nafiseh Maleki, Zahra Tahanasab, Sina Mobasherizadeh, Aliakbar Rezaei, Jamshid Faghri
Background: Extended-spectrum β-lactamase (ESBL)-producing is a significant resistant mechanism to β-lactams in Enterobacteriaceae , especially in Klebsiella pneumoniae . The main objectives of this study were to genetically characterize urinary clinical isolates of K. pneumoniae through the investigating of blaTEM , blaCTX-M and using molecular typing by Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) method. We also determined the frequency of antibiotic resistance of K...
2018: Advanced Biomedical Research
Fang Chen, Ling Wang, Min Wang, Yixin Xie, Xiaomeng Xia, Xianping Li, Yanhua Liu, Wei Cao, Tingting Zhang, Pengling Li, Min Yang
The present study aimed to develop a rational therapy based on the genetic epidemiology, molecular mechanism evaluation and in vitro antibiotic combinations activity in multidrug-resistant Acinetobacter baumannii (MDRAB). MDRAB was screened by the Kirby-Bauer method. The random amplified polymorphic DNA technique was used to establish genetic fingerprinting, and a series of resistance genes were detected by polymerase chain reaction. Antimicrobial agents including amikacin (AK), cefoperazone/sulbactam (SCF I/II), meropenem (MEM), minocycline (MINO) and ciprofloxacin (CIP) were used to determine the minimum inhibitory concentrations (MICs) and interactions between antibiotics by the broth microdilution method and chequerboard assays...
February 2018: Oncology Letters
K S Shabeena, Rahul Bhargava, Muhammed A P Manzoor, M Mujeeburahiman
Background: Indwelling Double-J ureteral stenting is commonly used in urological practice and has various complications. This study aimed to assess the frequency of bacterial stent colonization and stent-associated bacteriuria after indwelling it for different time durations and to evaluate the significance of urinary cultures for identification of colonizing microorganisms. Materials and Methods: A prospective cross-sectional study was conducted. Midstream urine from 72 patients undergoing J stent insertion was investigated microbiologically before stent insertion and on the day of stent removal...
January 2018: Urology Annals
Ivan Sserwadda, Mathew Lukenge, Bashir Mwambi, Gerald Mboowa, Apollo Walusimbi, Farouk Segujja
BACKGROUND: Nosocomial infections are a major setback in the healthcare delivery system especially in developing countries due to the limited resources. The roles played by medical care equipment and work surfaces in the transmission of such organisms have inevitably contributed to the elevated mortality, morbidity and antibiotic resistances. METHODS: A total 138 samples were collected during the study from Kawolo general hospital. Swab samples were collected from various work surfaces and fomites which consisted of; beds, sink taps, infusion stands, switches, work tables and scissors...
February 6, 2018: BMC Infectious Diseases
Shakeel Shahzad, M Adnan Ashraf, M Sajid, Aqeel Shahzad, Azhar Rafique, M Shahid Mahmood
BACKGROUND & OBJECTIVES: Multiple drug resistant super bugs of Acinetobacter baumannii and methicillin-resistant Staphylococcus aureus (MRSA) are becoming challenge for healthcare professionals. In this study, vitamins were evaluated for synergistic activity with the antibiotics. METHODS: Synergistic effect between antibiotic and stock solutions of vitamins is evaluated by using Kirby-Bauer disc diffusion assay. Distilled water and propylene glycol were used as solvent for water soluble vitamins and fat-soluble vitamins respectively...
February 2, 2018: Journal of Global Antimicrobial Resistance
Farhad Sharafati Chaleshtori, Mohamad Saholi, Reza Sharafati Chaleshtori
This research was aimed at investigating the antioxidant and antibacterial activity of Bunium persicum, Eucalyptus globulus, and rose water on multidrug-resistant Listeria species. The antibiotic resistance of Listeria spp obtained from seafood samples were determined by the Kirby-Bauer method. The antioxidant and antibacterial activity of the essential oils and extracts were evaluated using ferric reducing antioxidant power and microdilution methods, respectively. A total 2 samples (1.88%) were positive for Listeria spp...
January 2018: J Evid Based Integr Med
Puja Neopane, Hari Prasad Nepal, Rojeet Shrestha, Osamu Uehara, Yoshihiro Abiko
Introduction: Staphylococcus aureus including methicillin-resistant S. aureus (MRSA) has the propensity to form biofilms, and causes significant mortality and morbidity in the patients with wounds. Our aim was to study the in vitro biofilm-forming ability of S. aureus isolated from wounds of hospitalized patients and their association with antimicrobial resistance. Materials and methods: Forty-three clinical isolates of S. aureus were obtained from 150 pus samples using standard microbiological techniques...
2018: International Journal of General Medicine
Ronita Adams, Jackie Smith, Stephen Locke, Erica Phillips, Erdal Erol, Craig Carter, Agricola Odoi
BACKGROUND: Antimicrobial resistance limits traditional treatment options and increases costs. It is therefore important to estimate the magnitude of the problem so as to provide empirical data to guide control efforts. The aim of this study was to investigate the burden and patterns of antimicrobial resistance (AMR) among equine Staphylococcus samples submitted to the University of Kentucky Veterinary Diagnostic Laboratory (UKVDL) from 1993 to 2009. Retrospective data of 1711 equine Staphylococcus samples submitted to the UKVDL during the time period 1993 to 2009 were included in the study...
February 5, 2018: BMC Veterinary Research
M Stevens, S Piepers, K Supré, S De Vliegher
The main objectives of this study were to quantify the consumption of antimicrobials on a convenience sample of dairy herds and to determine the association between herd-level antimicrobial consumption and inhibition zone diameters (IZD) of non-aureus staphylococci and Staphylococcus aureus isolates from subclinical mastitis cases. Also, the association between the IZD of non-aureus staphylococci and Staph. aureus isolates within a herd was studied. Antimicrobial consumption data on 56 Flemish dairy farms were obtained between 2013 and 2014 by so-called garbage can audits and expressed as antimicrobial treatment incidence (ATI), with the unit of ATI being the number of defined daily doses animal (DDDA) used per 1,000 cow-days...
February 2, 2018: Journal of Dairy Science
Ghada H Naguib, Khaled M Hosny, Ali H Hassan, Faten Al Hazmi, Ayman Al Dharrab, Hala M Alkhalidi, Mohamed T Hamed, Abeer M Alnowaiser, David H Pashley
MgO nanoparticles have been recently discovered as an antibacterial, however, they limited by property degradation due to agglomeration. The addition of a coating agent, such as a zein polymer, is effective in preventing agglomeration without affecting nanosized properties. The aim of this study was to assess the antimicrobial property of MgO nanoparticles when coated with a zein polymer against several oral bacteria and fungi. This was done by utilizing various assessment techniques. The ultimate aim is to use these nanoparticles in dental preparations...
January 2018: Pakistan Journal of Pharmaceutical Sciences
Nisansala Swarnamali Bopage, G M Kamal Bandara Gunaherath, Kithsiri Hector Jayawardena, Sushila Chandrani Wijeyaratne, Ajita Mahendra Abeysekera, Seneviratne Somaratne
BACKGROUND: Different parts including the latex of Ficus racemosa L. has been used as a medicine for wound healing in the Ayurveda and in the indigenous system of medicine in Sri Lanka. This plant has been evaluated for its wound healing potential using animal models. The aim of this study was to obtain an insight into the wound healing process and identify the potential wound healing active substance/s present in F. racemosa L. bark using scratch wound assay (SWA) as the in-vitro assay method...
January 25, 2018: BMC Complementary and Alternative Medicine
Subhapriya S, Gomathi Priya Ponnaiah
In recent years, biosynthesis of nanoparticles has received considerable attention due to the growing need to develop clean and nontoxic chemicals, low-cost approaches, eco - friendly solvents and renewable materials. In the current study, the biosynthesis of TiO2 nanoparticles (TiO2NPs) was attained by a chemical and biosynthesized method by using the aqueous leaf extract of Trigonella foenum-graecum (TF-TiO2NP). TiO2 NPs were characterized by FTIR, UV, XRD, HR-TEM and HR-SEM methods. The X-ray diffraction displayed the existence of TF-TiO2NPs which is confirmed by the incidence of peaks at 25...
January 20, 2018: Microbial Pathogenesis
Ingvild Odsbu, Smita Khedkar, Frida Lind, Uday Khedkar, Sandeep S Nerkar, Nicola Orsini, Ashok J Tamhankar, Cecilia Stålsby Lundborg
Surveillance data on the level of resistant bacteria is needed to inform strategies to reduce the development and spread of antibiotic resistance. The aim of this study was to determine the non-susceptibility trends to extended-spectrum cephalosporins and carbapenems among Escherichia coli and Klebsiella spp. isolates from the district of Nashik in Western India during the period 2004-2014. Antibacterial susceptibility testing of clinical isolates was performed using Kirby-Bauer disc diffusion method to determine inhibitory zone diameters...
January 19, 2018: International Journal of Environmental Research and Public Health
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