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https://www.readbyqxmd.com/read/29151997/genetic-investigation-of-beta-lactam-associated-antibiotic-resistance-among-escherichia-coli-strains-isolated-from-water-sources
#1
Reza Ranjbar, Mehrdad Sami
Background: Antimicrobial resistance is an important factor threatening human health. It is widely accepted that antibiotic resistant bacteria such as Escherichia coli (E. coli) released from humans and animals into the water sources, can introduce their resistance genes into the natural bacterial community. Objective: The aim of this study was to investigate the prevalence of blaTEM, blaCTX, blaSHV, blaOXA and blaVEB associated-antibiotic resistance among E. coli bacteria isolated from different water resources in Iran...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/29151379/a-study-of-bacterial-pathogens-and-antibiotic-susceptibility-patterns-in-chronic-suppurative-otitis-media
#2
M R Mofatteh, F Shahabian Moghaddam, M Yousefi, M H Namaei
OBJECTIVE: To assess the frequency of bacterial agents in chronic suppurative otitis media and the antibiotic susceptibility patterns of isolates among patients. METHODS: A total of 185 patients clinically diagnosed with chronic suppurative otitis media were interviewed and middle-ear effusion samples were collected using sterile swabs. All bacterial isolates were identified by conventional microbiological methods. Antibiotic susceptibility patterns of the isolates were determined by Kirby-Bauer disc diffusion...
November 20, 2017: Journal of Laryngology and Otology
https://www.readbyqxmd.com/read/29141347/-antimicrobial-resistance-and-molecular-subtypes-of-salmonella-typhi-and-salmonella-paratyphoid-isolates-from-jiangsu-province-2012-2015
#3
X X Kong, C Dong, H Ji, Y Wang, C C Bao, X Huo, H M Qian
Objective: To retrospectively analyze the antimicrobial resistance phenotype and molecular typing characteristics of Salmonella (S.) typhi and S. paratyphi in Jiangsu province from 2012 to 2015. Methods: The samples were collected from typhoid and paratyphoid patients in Jiangsu province. The biochemical identification and serotyping were carried out after isolation and culture. Kirby-Bauer (K-B) testing was used to detect the drug susceptibility of the strains. The molecular typing characteristics of S. typhi and S...
November 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/29138101/primary-antibiotic-resistance-of-helicobacter-pylori-in-chinese-patients-a-multi-region-prospective-7-year-study
#4
Dong-Sheng Liu, You-Hua Wang, Zhi-Rong Zeng, Zhen-Yu Zhang, Hong Lu, Jian-Ming Xu, Yi-Qi Du, Yan Li, Jiang-Bin Wang, San-Ping Xu, Ye Chen, Chun-Hui Lan, Hong Cheng, Ming-de Jiang, Ling-Xia Zhang, Li-Juan Huo, Shi-Yao Chen, Guo-Xin Zhang, Kai-Chun Wu, Xuan Zhu, You-Xiang Chen, Yin Zhu, Xu Shu, Yong Xie, Nong-Hua Lu
OBJECTIVES: To explore the characteristics of H. pylori resistance in China and the association between antibiotic resistance and several clinical factors. METHODS: H. pylori strains were collected from patients in 13 provinces/cities in China between 2010 and 2016. Demographic data including type of disease, geographic area, age, gender and isolation year were collected to analyse their association with antibiotic resistance. Antibiotic resistance was detected using the Epsilometer test and the Kirby-Bauer disk diffusion method...
November 11, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29132479/current-trends-of-antimicrobial-susceptibility-of-typhoidal-salmonellae-isolated-at-tertiary-care-hospital
#5
Nadia Midhat Zehra, Fatima Irfan, Irfan Ali Mirza, Anam Imtiaz, Saman Nadeem, Fatima Hameed
OBJECTIVE: To determine the current trend of antimicrobial susceptibility of typhoidal Salmonellae. STUDY DESIGN: Descriptive cross-sectional study. PLACE AND DURATION OF STUDY: The study was carried out in the Department of Microbiology, PNS Shifa Hospital, Karachi, from January 2014 to December 2015. METHODOLOGY: Blood culture samples received from the wards and outpatient departments were included. Isolates of Salmonella were dealt with standard microbiological procedures...
November 2017: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://www.readbyqxmd.com/read/29119726/cotrimoxazole-prophylaxis-was-associated-with-enteric-commensal-bacterial-resistance-among-hiv-exposed-infants-in-a-randomized-controlled-trial-botswana
#6
Kathleen M Powis, Sajini Souda, Shahin Lockman, Gbolahan Ajibola, Kara Bennett, Jean Leidner, Michael D Hughes, Sikhulile Moyo, Erik van Widenfelt, Haruna B Jibril, Joseph Makhema, Max Essex, Roger L Shapiro
INTRODUCTION: Despite declining risk of vertical HIV transmission, prophylactic cotrimoxazole (CTX) remains widely used to reduce morbidity and mortality in the event of HIV infection among exposed infants, with an inherent risk of conferring commensal antimicrobial resistance. Using data from a randomized, placebo-controlled trial of infant CTX prophylaxis, we sought to quantify emergence of antibiotic resistance. METHODS: HIV-exposed uninfected infants enrolled in the Botswana Mpepu study were randomized to prophylactic CTX or placebo between 14 and 34 days of life and continued through 15 months...
November 2017: Journal of the International AIDS Society
https://www.readbyqxmd.com/read/29102052/reduction-of-bacterial-burden-by-copper-alloys-on-high-touch-athletic-center-surfaces
#7
Zina Ibrahim, Alexandra J Petrusan, Patrick Hooke, Shannon M Hinsa-Leasure
BACKGROUND: Athletic centers have been locations for the transmission of community-acquired infections. This study assessed the capacity of copper alloys to reduce the bacterial burden associated with high-touch athletic center equipment. Copper alloy weights and grips were rotated with rubber coated and stainless steel controls in an undergraduate college athletic center over a 16-month period. The athletic center is used by college athletic teams, student body, and local community. METHODS: The primary outcome was to compare bacterial burdens on copper and control grips by swabbing surfaces...
October 6, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/29096652/biofilm-and-metallo-beta-lactamase-production-among-the-strains-of-pseudomonas-aeruginosa-and-acinetobacter-spp-at-a-tertiary-care-hospital-in-kathmandu-nepal
#8
Bandana Baniya, Narayan Dutt Pant, Sanjeev Neupane, Saroj Khatiwada, Uday Narayan Yadav, Nisha Bhandari, Rama Khadka, Sabita Bhatta, Raina Chaudhary
INTRODUCTION: Pseudomonas aeruginosa and Acinetobacter spp. are found to be associated with biofilm and metallo-β-lactamase production and are the common causes of serious infections mainly in hospitalized patients. So, the main aims of this study were to determine the rates of biofilm production and metallo beta-lactamase production (MBL) among the strains of Pseudomonas aeruginosa and Acinetobacter spp. isolated from hospitalized patients. METHODS: A total of 85 P...
November 2, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29075483/extended-spectrum-and-metalo-beta-lactamase-producing-airborne-pseudomonas-aeruginosa-and-acinetobacter-baumanii-in-restricted-settings-of-a-referral-hospital-a-neglected-condition
#9
Fithamlak Bisetegen Solomon, Fiseha Wadilo, Efrata Girma Tufa, Meseret Mitiku
BACKGROUND: Frequently encountered multidrug-resistant bacterial isolates of P. aeruginosa and A. baumannii are common and prevalent in a hospital environment. The aim of this study was to determine the prevalence and pattern of antibiotic resistance, extended spectrum and metallo beta-lactamase producing P. aeruginosa and A. baumannii isolates from restricted settings of indoor air hospital environment. METHODS: A hospital-based cross-sectional study was conducted in Wolaita Sodo University Teaching and referral Hospital, Ethiopia from December 1/2015 to April 30/2015...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29071076/burden-of-bacterial-upper-respiratory-tract-pathogens-in-school-children-of-nepal
#10
Sangita Thapa, Shishir Gokhale, Annavarapu Laxminarasimha Sharma, Lokendra Bahadur Sapkota, Shamshul Ansari, Rajendra Gautam, Sony Shrestha, Puja Neopane
INTRODUCTION: Respiratory tract infections are one of the most common human infections in all age group and important cause of mortality and morbidity worldwide. Most bacterial upper respiratory tract infections are vaccine preventable. This study aimed to determine the prevalence of carrier state of bacterial upper respiratory tract pathogens among school children. It also aimed to study their antibiograms. METHODS: The specimen from posterior pharyngeal wall and tonsils were collected from 204 participants on calcium alginate coated swabs (HiMedia)...
2017: BMJ Open Respiratory Research
https://www.readbyqxmd.com/read/29067059/phenotypic-characterization-of-extended-spectrum-beta-lactamase-producing-e-coli-from-healthy-individuals-patients-sewage-sludge-cattle-chickens-and-raw-meat
#11
Rabia Saleem, Hasan Ejaz, Aizza Zafar, Sonia Younas, Ahsan Waheed Rathore
OBJECTIVE: The present study aimed to determine the frequency and antimicrobial profile of ESBL-producing isolates of E. coli in different environments. METHODS: This cross-sectional study was conducted at The Children's Hospital and The Institute of Child Health, Lahore from July to December 2015. The faecal specimens from healthy individuals, patients, sewage sludge, cattle, chickens and raw meat (n = 122) were processed for microbiological analysis using MacConkey agar supplemented with cefotaxime...
July 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/29038627/characterization-and-antibiotic-sensitivity-profile-of-bacteria-in-orofacial-abscesses-of-odontogenic-origin
#12
H Jagadish Chandra, B H Sripathi Rao, A P Muhammed Manzoor, A B Arun
BACKGROUND: Odontogenic infections range from peripheral abscess to superficial and deep infections leading to severe infections in head and neck region. This study was aimed to assess bacterial isolates responsible for orofacial infection of odontogenic origin and their drug susceptibility patterns so as to provide better perceptive for the management of odontogenic infections. METHODS: The study was made in a selected cohort of patients, irrespective of age and gender having moderate and severe orofacial infections of odontogenic origin admitted to Yenepoya University Hospital...
December 2017: Journal of Maxillofacial and Oral Surgery
https://www.readbyqxmd.com/read/29026795/the-prevalence-of-plasmid-mediated-quinolone-resistance-genes-in-escherichia-coli-isolated-from-hospital-wastewater-sources-in-tehran-iran
#13
Reza Ranjbar, Omid Farahani
BACKGROUND: Considering the importance of hospital wastewaters as potential reservoirs for the dissemination of bacterial pathogens such as Escherichia coli and antibiotic resistance genes in the environment, the need for such information becomes imperative. METHODS: E. coli strains were isolated from hospital wastewater sources in Tehran, Iran, over a 24-month sampling period (Jun 2014- Jun 2016) and identified using standard bacteriological methods. Quinolone resistance among the strains was determined using Kirby-Bauer method and the frequency of plasmid-mediated quinolone resistance genes (qnrA, qnrB, qnrS) was investigated by PCR...
September 2017: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/29021723/bacterial-infections-in-burn-wound-patients-at-a-tertiary-teaching-hospital-in-accra-ghana
#14
O A Forson, E Ayanka, M Olu-Taiwo, P J Pappoe-Ashong, P J Ayeh-Kumi
Intact human skin surface is essential for protection against infection, preservation of body fluid homeostasis and thermoregulation. Burn injury compromises the skin barrier and enables bacterial infection, hence delaying burn wound healing. This study aimed to determine the microbial profile of burn wounds, and resistance patterns of microbes with respect to the source of the injured patient's wound. Fifty wound swab samples were collected from fifty burn patients at the Korle-Bu Teaching Hospital, Accra (KBTH)...
June 30, 2017: Annals of Burns and Fire Disasters
https://www.readbyqxmd.com/read/29018810/renaissance-of-conventional-first-line-antibiotics-in-salmonella-enterica-clinical-isolates-assessment-of-mics-for-therapeutic-antimicrobials-in-enteric-fever-cases-from-nepal
#15
Puspa Raj Khanal, Deepa Satyal, Anjeela Bhetwal, Anjila Maharjan, Shreena Shakya, Snehika Tandukar, Narayan Prasad Parajuli
Enteric fever caused by Salmonella enterica is a life-threatening systemic illness of gastrointestinal tract especially in tropical countries. Antimicrobial therapy is generally indicated but resistance towards commonly used antibiotics has limited their therapeutic usefulness. Therefore, we aimed to determine the antimicrobial susceptibility pattern by minimum inhibitory concentration method of common therapeutic regimens against Salmonella enterica from enteric fever clinical cases. Salmonella enterica clinical isolates recovered from the patients with suspected enteric fever whose blood samples were submitted to microbiology laboratory of Manmohan Memorial Community Hospital, Kathmandu, from March 2016 to August 2016, were studied...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29016585/isolation-of-multidrug-resistant-bacteria-from-patients-medical-charts
#16
Roshan Thapa, Narayan Dutt Pant, Uday Narayan Yadav, Eliza Thapa, Anjana Singh, Bharat Mani Pokhrel, Upendra Devkota
BACKGROUND: Patient's medical charts in hospitals are potentially contaminated by pathogenic bacteria and might act as vehicles for transmission of bacterial infections.This study was aimed to determine the rate of contamination of medical charts by multidrug resistant bacteria. METHODS: Sampling of total 250 patient's medical charts from different wards was done with the help of cotton swabs soaked in sterile normal saline. The swabs thus collected were cultured using standard microbiological procedures...
September 8, 2017: Journal of Nepal Health Research Council
https://www.readbyqxmd.com/read/29016581/bacteriological-study-of-otitis-media-and-its-antibiotic-susceptibility-pattern
#17
Rabina Basnet, Sudarshan Sharma, Jid Chani Rana, Pradeep Kumar Shah
BACKGROUND: Otitis Media is a prevailing and notorious infection in developing countries causing serious local damage and threating complication. Mainly in developing countries like Nepal, Otitis Media results because of illiteracy, poverty and poor hygiene. The aim of this study was to determine the profile of Otitis Media, its causative agents and their antibiotic susceptibility pattern. METHODS: The study included 263 pus samples from 240 patients attending ENT department of Bharatpur hospital from May 2015 to January 2016...
September 8, 2017: Journal of Nepal Health Research Council
https://www.readbyqxmd.com/read/29016580/surgical-site-infections-and-antimicrobial-resistance-pattern
#18
Raskin Chaudhary, Shrawan Kumar Thapa, Jid Chani Rana, Pradeep Kumar Shah
BACKGROUND: Post-operative surgical site infections (SSIs) are among the leading cause of morbidity and increased medical expense. The aim of this study is to isolate identify and study antimicrobial susceptibility pattern of microorganism from surgical wound of admitted patients. METHODS: This retrospective study was carried at the Microbiology Laboratory of Bharatpur hospital, Nepal, from May 2015 to October 2015. The pus samples were cultured and antibiotic susceptibility determined in vitro by Kirby Bauer's disc diffusion method following clinical and Laboratory Standards Institute (CLSI) 2014 recommendation...
September 8, 2017: Journal of Nepal Health Research Council
https://www.readbyqxmd.com/read/28980098/prevalence-of-bovine-subclinical-mastitis-and-antibiotic-susceptibility-patterns-of-major-mastitis-pathogens-isolated-in-unguja-island-of-zanzibar-tanzania
#19
T S Suleiman, E D Karimuribo, R H Mdegela
A cross-sectional study was conducted between January and July 2014 in Unguja island of Zanzibar to establish prevalence of subclinical mastitis (SCM) in smallholder dairy cows and patterns of antibacterial susceptibility of major mastitis pathogens isolated. A total of 416 dairy cows from 201 farmers were randomly selected from three districts of Unguja Island to participate in the study. Questionnaire interview, field observation, individual cow examination, California Mastitis Test (CMT) and bacteriological examination were carried out...
October 4, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28975103/evaluating-the-resistance-pattern-of-gram-negative-bacteria-during-three-years-at-the-nephrology-ward-of-a-referral-hospital-in-southwest-of-iran
#20
Iman Karimzadeh, Niloofar Sadeghimanesh, Mona Mirzaee, Mohammad Mahdi Sagheb
BACKGROUND: Gram-negative bacteria are associated with an increase in rates of antibacterial resistance. In most low- and middle-income countries such as Iran, there is no continuous surveillance system for antibiotic resistance. OBJECTIVE: The purpose of this survey was to determine the pattern of antimicrobial sensitivity of gram-negative bacteria within 3 consecutive years at a nephrology ward of Nemazee hospital in Shiraz. MATERIALS AND METHODS: During a 3-year period from 2013 to 2015 at the adult nephrology ward, bacteriological data of all biological samples of hospitalized patients in favor of gram-negative microorganisms were analyzed retrospectively...
July 2017: Journal of Nephropathology
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