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https://www.readbyqxmd.com/read/29733799/asymptomatic-bacteriuria-and-antimicrobial-susceptibility-pattern-of-the-isolates-among-pregnant-women-attending-dessie-referral-hospital-northeast-ethiopia-a-hospital-based-cross-sectional-study
#1
Ismail Ebrie Ali, Teklay Gebrecherkos, Mucheye Gizachew, Martha Alemayehu Menberu
OBJECTIVE: The aim of the current study was to find out the prevalence of asymptomatic bacteriuria, antimicrobial susceptibility pattern of the isolates and related risk factors among pregnant women. MATERIAL AND METHODS: A hospital-based cross-sectional study was carried out from February 2017 to May 2017 among asymptomatic pregnant mothers attending Dessie Referral Hospital, Northeast Ethiopia. About 10-15 mL of freshly voided midstream urine samples were collected from each study participants, and analyzed at Dessie regional research microbiology laboratory with conventional antibiotic susceptibility, and biochemical tests...
May 2018: Turkish Journal of Urology
https://www.readbyqxmd.com/read/29730068/biofilm-testing-of-microbiota-an-essential-step-during-corneal-scrap-examination-in-egyptian-acanthamoebic-keratitis-cases
#2
Marwa A Hasby Saad, Haidy S M Khalil
PURPOSE: To detect co-infections in the culture-proven acanthamoebic keratitis (AK) cases, and to test the capability of biofilm formation in the isolated microbiota. The clinical findings, habit of wearing contact lens and in-vitro antibiotic resistance were analyzed further according to the biofilm formation capability. METHODS: After clinical examination, corneal scraps and swabs were taken from 240 clinically suspected AK cases, for Acanthamoeba and microbiological cultures...
May 3, 2018: Parasitology International
https://www.readbyqxmd.com/read/29703789/bacteria-profile-and-antibiogram-of-the-bacteria-isolated-from-the-exposed-pulp-of-dog-canine-teeth
#3
José Carlos Almansa Ruiz, Annelize Jonker, Anna-Mari Bosman, Gerhard Steenkamp
Twenty-seven microbiological samples were taken from root canals (RC) of the canine teeth of 20 dogs where the pulps were non-vital and exposed due to complicated crown fractures. These pulps were cultured for aerobic/anaerobic bacteria. Antimicrobial susceptibility of isolates was determined using the Kirby-Bauer diffusion test. A total of 49 cultivable isolates, belonging to 27 different microbial species and 18 different genera, were recovered from the 27 RCs sampled. Twenty (40.81 per cent) of the cultivable isolates were Gram positive while 29 (59...
April 27, 2018: Veterinary Record
https://www.readbyqxmd.com/read/29698291/csa-90-promotes-bone-formation-and-mitigates-methicillin-resistant-staphylococcus-aureus-infection-in-a-rat-open-fracture-model
#4
Rebecca Mills, Tegan L Cheng, Kathy Mikulec, Lauren Peacock, David Isaacs, Carl Genberg, Paul B Savage, David G Little, Aaron Schindeler
BACKGROUND: Infection of open fractures remains a significant cause of morbidity and mortality to patients worldwide. Early administration of prophylactic antibiotics is known to improve outcomes; however, increasing concern regarding antimicrobial resistance makes finding new compounds for use in such cases a pressing area for further research. CSA-90, a synthetic peptidomimetic compound, has previously demonstrated promising antimicrobial action against Staphylococcus aureus in rat open fractures...
April 24, 2018: Clinical Orthopaedics and related Research
https://www.readbyqxmd.com/read/29692474/molecular-detection-of-bla-ndm-1-new-delhi-metallobetalactamase-1-in-nosocomial-enterobacteriaceae-isolates-by-nested-multiplex-polymerase-chain-reaction
#5
Parnika Chandola, R M Gupta, Mahima Lall, Sourav Sen, S P S Shergill, Vibha Dutta
Background: Carbapenems are considered "drugs of last resort" in many life-threatening infections. Advent of carbapenemases like KPC, OXA-48, VIM, IMP, and NDM have greatly affected the efficacy of these drugs, posing serious threat to global health and infection control. NDM bears special significance to the India subcontinent, labeled as place of origin and reservoir. NDM tends to escape detection by routine phenotypic methods, requiring molecular confirmation. This study utilizes nested, multiplex polymerase chain reaction (PCR) for reliable detection of bla NDM-1 in nosocomial Enterobacteriaceae isolates...
April 2018: Medical Journal, Armed Forces India
https://www.readbyqxmd.com/read/29657925/biofilm-formation-in-nonmultidrug-resistant-escherichia-coli-isolated-from-patients-with-urinary-tract-infection-in-isfahan-iran
#6
Farkhondeh Poursina, Shima Sepehrpour, Sina Mobasherizadeh
Background: Escherichia coli is a Gram-negative, opportunistic human pathogen in which increasing antibiotic resistance is a great concern for continued human survival. Although biofilm formation is a mechanism that helps E. coli to survive in unfavorable conditions, according to the importance of biofilm formation in developing the antibiotic resistance here, we studied the relation between antibiotic resistance and in vitro qualitative rating method biofilm formation in E. coli isolated from patients with urinary tract infection (UTI)...
2018: Advanced Biomedical Research
https://www.readbyqxmd.com/read/29615166/phenotypic-identification-frequency-distribution-and-antibiogram-of-carbapenemase-producing-enterobacteriaceae-in-clinical-isolates
#7
Maliha Ansari, Tehmina Munir, Nadia Saad
OBJECTIVE: To differentiate between Ambler class A, B and D of carbapenemase-producing Enterobacteriaceae by using simple phenotypic methods that can be carried out in the laboratory without requiring any specialised techniques. STUDY DESIGN: Cross-sectional study. PLACE AND DURATION OF STUDY: Microbiology Department, Army Medical College, NUST, Islamabad, from November 2015 to November 2016. METHODOLOGY: Clinical specimens were subjected to identification of Enterobacteriaceae by colony morphology and API 20 E...
April 2018: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://www.readbyqxmd.com/read/29577253/antimicrobial-susceptibility-testing-by-australian-veterinary-diagnostic-laboratories
#8
L Y Hardefeldt, M Marenda, H Crabb, M A Stevenson, J R Gilkerson, H Billman-Jacobe, G F Browning
BACKGROUND: The national strategy for tackling antimicrobial resistance highlights the need for antimicrobial stewardship in veterinary practice and for surveillance of antimicrobial susceptibility in veterinary pathogens. Diagnostic laboratories have an important role in facilitating both of these processes, but it is unclear whether data from veterinary diagnostic laboratories are similar enough to allow for compilation and if there is consistent promotion of appropriate antimicrobial use embedded in the approaches of different laboratories to susceptibility testing...
April 2018: Australian Veterinary Journal
https://www.readbyqxmd.com/read/29567221/systematic-study-on-active-compounds-as-antibacterial-and-antibiofilm-agent-in-aging-onions
#9
Kavita Sharma, Neelima Mahato, Yong Rok Lee
Quantitative investigation and systematic studies of quercetin, total phenolics, flavonoids, antioxidants, antibacterial and antibiofilm or antibiofouling properties of methanolic extracts of onions obtained from six different varieties have been carried out to explore their relative merits in terms of biological activities of fresh and aging onions. Total phenolic content in the extracts was examined spectrophotometrically using Folin-Ciocalteau's phenol reagent and total antioxidant activity was studied by FRAP and DPPH methods...
April 2018: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/29564239/prevalence-and-characterisation-of-shigatoxigenic-escherichia-coli-isolated-from-beef-cattle-fed-with-prebiotics
#10
Luca Grispoldi, Filippo Bertero, Serena Franceschini, Francesco Mastrosimone, Paola Sechi, Maria Francesca Iulietto, Margherita Ceccarelli, Beniamino Terzo Cenci-Goga
Ten Holstein Friesian calves were divided into two groups of five: one group was given prebiotics in their food, while the other group served as the control group. Every two weeks from birth up to 18 months, samples of feces were taken from the rectal ampulla to determine the concentration of E. coli . At each sampling session, three aliquots per sample were collected. The arithmetic mean was calculated and all values (converted into logs) were analysed with GraphPad InStat for analysis of variance, followed by the Tukey-Kramer test...
October 20, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29547034/effects-of-ph-on-the-antibiotic-resistance-of-bacteria-recovered-from-diabetic-foot-ulcer-fluid-an-in-vitro-study
#11
Carla D McArdle, Katie M Lagan, David A McDowell
BACKGROUND: This study investigated the resistance of bacteria isolated from diabetic foot ulcers (DFUs) to antibiotics frequently used in the management of the diabetic foot infections, at a range of pH values (pH 6.5, 7.5, and 8.5) known to exist in DFU wound fluid. This study aimed to determine whether changes (or atypical stasis) in wound fluid pH modulate the antibiotic resistance of DFU isolates, with potential implications in relation to the suppression/eradication of bacterial infections in DFUs...
January 2018: Journal of the American Podiatric Medical Association
https://www.readbyqxmd.com/read/29527123/can-disc-diffusion-susceptibility-tests-assess-the-antimicrobial-activity-of-engineered-nanoparticles
#12
Angeliki Kourmouli, Marco Valenti, Erwin van Rijn, Hubertus J E Beaumont, Olga-Ioanna Kalantzi, Andreas Schmidt-Ott, George Biskos
The use of disc diffusion susceptibility tests to determine the antibacterial activity of engineered nanoparticles (ENPs) is questionable because their low diffusivity practically prevents them from penetrating through the culture media. In this study, we investigate the ability of such a test, namely the Kirby-Bauer disc diffusion test, to determine the antimicrobial activity of Au and Ag ENPs having diameters from 10 to 40 nm on Escherichia coli cultures. As anticipated, the tests did not show any antibacterial effects of Au nanoparticles (NPs) as a result of their negligible diffusivity through the culture media...
2018: Journal of Nanoparticle Research: An Interdisciplinary Forum for Nanoscale Science and Technology
https://www.readbyqxmd.com/read/29503643/multidrug-resistant-escherichia-albertii-co-occurrence-of-%C3%AE-lactamase-and-mcr-1-encoding-genes
#13
Qun Li, Hong Wang, Yanmei Xu, Xiangning Bai, Jianping Wang, Zhengdong Zhang, Xiang Liu, Yimao Miao, Ling Zhang, Xinqiong Li, Nianli Zou, Guodong Yan, Xi Chen, Jie Zhang, Shanshan Fu, Ruyue Fan, Jianguo Xu, Juan Li, Yanwen Xiong
Escherichia albertii is an emerging member of the Enterobacteriaceae causing human and animal enteric infections. Antimicrobial resistance among enteropathogens has been reported to be increasing in the past years. The purpose of this study was to investigate antibiotic resistance and resistance genes in E. albertii isolated from Zigong city, Sichuan province, China. The susceptibility to 21 antimicrobial agents was determined by Kirby-Bauer disk diffusion method. The highest prevalence was tetracycline resistance with a rate of 62...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29496594/bla-imp-27-on-transferable-plasmids-in-proteus-mirabilis-and-providencia-rettgeri
#14
R F Potter, M A Wallace, A R McMullen, J Prusa, C L Stallings, C A D Burnham, G Dantas
OBJECTIVES: A carbapenem-resistant Providencia rettgeri (PR1) isolate was recovered from a wound infection in Missouri, USA. This isolate possessed an EDTA-inhibitable carbapenemase that was unidentified using the Xpert CARBA-R assay. Our objective was to elucidate the molecular determinant of carbapenem resistance in this isolate. We then sought to test the transmissibility of blaIMP-27 loci in clinical P. rettgeri and Proteus mirabilis isolates. METHODS: In October 2016 the novel ambler Class B carbapenemase blaIMP-27 , was reported in two different Proteus mirabilis (PM185 and PM187) isolates...
March 1, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29495161/-study-on-antimicrobial-susceptibility-of-brucella-in-a-city
#15
S W Zuo, Z L Ni, Y B Yao, R S Yang, S K Wang, Y H Zhou
Objective: To investigate the antimicrobial susceptibility of Brucella and to provide a scientific basis for rational drug use and effective treatment of patients with brucellosis. Methods: A total of 41 Brucella strains were isolated from the blood of patients with brucellosis in 5 counties and 2 districts in Yuxi City, China from 2014 to 2016. The susceptibility to 23 antimicrobial drugs was tested using Kirby-Bauer (K-B) disk diffusion method and the sizes of antimicrobial rings were recorded. The susceptibility testing results were interpreted according to the Drug Susceptibility Testing Guideline (2009 version) ...
December 20, 2017: Chinese Journal of Industrial Hygiene and Occupational Diseases
https://www.readbyqxmd.com/read/29475651/effects-of-glyphosate-herbicide-on-the-gastrointestinal-microflora-of-hawaiian-green-turtles-chelonia-mydas-linnaeus
#16
Ronald P Kittle, Karla J McDermid, Lisa Muehlstein, George H Balazs
In Hawaii, glyphosate-based herbicides frequently sprayed near shorelines may be affecting non-target marine species. Glyphosate inhibits aromatic amino acid biosynthesis (shikimate pathway), and is toxic to beneficial gut bacteria in cattle and chickens. Effects of glyphosate on gut bacteria in marine herbivorous turtles were assessed in vitro. When cultures of mixed bacterial communities from gastrointestinal tracts of freshly euthanized green turtles (Chelonia mydas), were exposed for 24h to six glyphosate concentrations (plus deionized water control), bacterial density was significantly lower at glyphosate concentrations≥2...
February 2018: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/29471138/antibacterial-activities-of-tannic-acid-against-isolated-ruminal-bacteria-from-sheep
#17
Moisés Cipriano-Salazar, Saúl Rojas-Hernández, Jaime Olivares-Pérez, Régulo Jiménez-Guillén, Blas Cruz-Lagunas, Luis Miguel Camacho-Díaz, Amadike Eziuche Ugbogu
This present study was conducted to evaluate the antimicrobial effects of tannic acid (TA) against isolated ruminal bacteria from adult sheep. Rumen samples were collected from two (2) adults sheep, and a total of nine (9) ruminal bacteria were isolated from the sample. The sensitivity of the ruminal bacteria isolates to 0.63, 1.25, 2.50, 5.00 and 10.00 mg TA/mL of growth medium was determined using clearance zone (CZ) of Kirby-Bauer disc diffusion susceptibility test. There was observable increase in the sensitivity of all bacterial isolates as the level of TA increases...
April 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29456981/prevalence-of-ctx-m-and-tem-%C3%AE-lactamases-in-klebsiella-pneumoniae-isolates-from-patients-with-urinary-tract-infection-al-zahra-hospital-isfahan-iran
#18
Nafiseh Maleki, Zahra Tahanasab, Sina Mobasherizadeh, Aliakbar Rezaei, Jamshid Faghri
Background: Extended-spectrum β-lactamase (ESBL)-producing is a significant resistant mechanism to β-lactams in Enterobacteriaceae , especially in Klebsiella pneumoniae . The main objectives of this study were to genetically characterize urinary clinical isolates of K. pneumoniae through the investigating of blaTEM , blaCTX-M and using molecular typing by Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) method. We also determined the frequency of antibiotic resistance of K...
2018: Advanced Biomedical Research
https://www.readbyqxmd.com/read/29434938/genetic-characterization-and-in-vitro-activity-of-antimicrobial-combinations-of-multidrug-resistant-acinetobacter-baumannii-from-a-general-hospital-in-china
#19
Fang Chen, Ling Wang, Min Wang, Yixin Xie, Xiaomeng Xia, Xianping Li, Yanhua Liu, Wei Cao, Tingting Zhang, Pengling Li, Min Yang
The present study aimed to develop a rational therapy based on the genetic epidemiology, molecular mechanism evaluation and in vitro antibiotic combinations activity in multidrug-resistant Acinetobacter baumannii (MDRAB). MDRAB was screened by the Kirby-Bauer method. The random amplified polymorphic DNA technique was used to establish genetic fingerprinting, and a series of resistance genes were detected by polymerase chain reaction. Antimicrobial agents including amikacin (AK), cefoperazone/sulbactam (SCF I/II), meropenem (MEM), minocycline (MINO) and ciprofloxacin (CIP) were used to determine the minimum inhibitory concentrations (MICs) and interactions between antibiotics by the broth microdilution method and chequerboard assays...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29416279/characteristics-of-bacterial-colonization-after-indwelling-double-j-ureteral-stents-for-different-time-duration
#20
K S Shabeena, Rahul Bhargava, Muhammed A P Manzoor, M Mujeeburahiman
Background: Indwelling Double-J ureteral stenting is commonly used in urological practice and has various complications. This study aimed to assess the frequency of bacterial stent colonization and stent-associated bacteriuria after indwelling it for different time durations and to evaluate the significance of urinary cultures for identification of colonizing microorganisms. Materials and Methods: A prospective cross-sectional study was conducted. Midstream urine from 72 patients undergoing J stent insertion was investigated microbiologically before stent insertion and on the day of stent removal...
January 2018: Urology Annals
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