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https://www.readbyqxmd.com/read/28820687/identification-of-subsets-of-enteroaggregative-escherichia-coli-associated-with-diarrheal-disease-among-under-5-year-old-children-from-rural-gambia
#1
Usman N Ikumapayi, Nadia Boisen, Mohammad J Hossain, Modupeh Betts, Modou Lamin, Debasish Saha, Brenda Kwambana-Adams, Michel Dione, Richard A Adegbola, Anna Roca, James P Nataro, Martin Antonio
Enteroaggregative Escherichia coli (EAEC) cause acute and persistent diarrhea, mostly in children worldwide. Outbreaks of diarrhea caused by EAEC have been described, including a large outbreak caused by a Shiga toxin expressing strain. This study investigated the association of EAEC virulence factors with diarrhea in children less than 5 years. We characterized 428 EAEC strains isolated from stool samples obtained from moderate-to-severe diarrhea cases (157) and healthy controls (217) children aged 0-59 months recruited over 3 years as part of the Global Enteric Multicenter Study (GEMS) in The Gambia...
August 7, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28814888/frequency-of-colistin-and-fosfomycin-resistance-in-carbapenem-resistant-enterobacteriaceae-from-a-tertiary-care-hospital-in-karachi
#2
Salima Qamar, Najma Shaheen, Sadia Shakoor, Joveria Farooqi, Kauser Jabeen, Rumina Hasan
INTRODUCTION: Management of infections with carbapenem-resistant Enterobacteriaceae (CRE) is challenging. In recent times, agents such as colistin and fosfomycin have been used in combination with other antibiotics to treat such infections. In this study, we aim to seek frequency of colistin and fosfomycin resistance in CRE from Pakistan. METHODS: This study was conducted at clinical laboratories, Aga Khan University Hospital. In total, 251 CRE were included in the study...
2017: Infection and Drug Resistance
https://www.readbyqxmd.com/read/28812009/the-bacteriological-quality-safety-and-antibiogram-of-salmonella-isolates-from-fresh-meat-in-retail-shops-of-bahir-dar-city-ethiopia
#3
Melkamnesh Azage, Mulugeta Kibret
The habit of raw meat consumption in addition to the poor hygienic standards and lack of knowledge contribute to food-borne diseases outbreaks. The objective of this research was to assess the bacterial quality and safety of fresh meat from retail Bahir Dar City, Ethiopia. A total of 30 fresh meat samples were collected from butcher shops. Standard bacteriological methods were used to isolate and enumerate bacteria. Kirby-Bauer disk diffusion method was used for antimicrobial susceptibility testing of Salmonella isolates...
2017: International journal of food science
https://www.readbyqxmd.com/read/28811792/antimicrobial-susceptibility-patterns-of-clinical-isolates-of-pseudomonas-aeruginosa-isolated-from-patients-of-respiratory-tract-infections-in-a-tertiary-care-hospital-peshawar
#4
Abdul Samad, Tanveer Ahmed, Afaq Rahim, Abdul Khalil, Iftikhar Ali
OBJECTIVE: This study aims to determine the prevalence and susceptibility pattern of Pseudomonas aeruginosa and multidrug-resistant (MDR) isolates in patients suffering from respiratory tract infection. METHODS: A cross sectional study was conducted from January to December 2014 in Northwest General Hospital and Research Centre, Peshawar. A total of 615 sputum samples were collected from both in and out-patients. Sputum samples were collected as per standard procedure and were inoculated on Blood, MacConkey and Chocolate agar...
May 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/28810329/-distribution-and-drug-resistance-of-pathogens-at-hematology-department-of-jiangsu-province-from-2014-to-2015-results-from-a-multicenter-retrospective-study
#5
Y K Wan, W Sang, B Chen, Y G Yang, L Q Zhang, A N Sun, Y J Liu, Y Xu, Y P Cai, C B Wang, Y F Shen, Y W Jiang, X Y Zhang, W Xu, M Hong, T Chen, R R Xu, F Li, Y L Xu, Y Xue, Y L Lu, Z M He, W M Dong, Z Chen, M H Ji, Y Y Yang, L J Zhai, Y Zhao, G Q Wu, J H Ding, J Cheng, W B Cai, Y M Sun, J Ouyang
Objective: To describe the distribution and drug resistance of pathogens at hematology department of Jiangsu Province from 2014 to 2015 to provide reference for empirical anti-infection treatment. Methods: Pathogens were from hematology department of 26 tertiary hospitals in Jiangsu Province from 2014 to 2015. Antimicrobial susceptibility testing was carried out according to a unified protocol using Kirby-Bauer method or agar dilution method. Collection of drug susceptibility results and corresponding patient data were analyzed...
July 14, 2017: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/28800411/antimicrobial-resistance-and-resistance-genes-in-salmonella-strains-isolated-from-broiler-chickens-along-the-slaughtering-process-in-china
#6
Yuanting Zhu, Haimei Lai, Likou Zou, Sheng Yin, Chengtao Wang, Xinfeng Han, Xiaolong Xia, Kaidi Hu, Li He, Kang Zhou, Shujuan Chen, Xiaolin Ao, Shuliang Liu
A total of 189 Salmonella isolates were recovered from 627 samples which were collected from cecal contents of broilers, chicken carcasses, chicken meat after cutting step and frozen broiler chicken products along the slaughtering process at a slaughterhouse in Sichuan province of China. The Salmonella isolates were subjected to antimicrobial susceptibility testing to 10 categories of antimicrobial agents using the Kirby-Bauer disk diffusion method. Those antibiotics-resistant isolates were further investigated for the occurrence of resistance genes, the presence of class 1 integron as well as the associated gene cassettes, and the mutations within the gyrA and parC genes...
August 1, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28792540/antibiotics-resistance-in-el-tor-vibrio-cholerae-01-isolated-during-cholera-outbreaks-in-mozambique-from-2012-to-2015
#7
Liliana Candida Dengo-Baloi, Cynthia Amino Semá-Baltazar, Lena Vania Manhique, Jucunu Elias Chitio, Dorteia Luísa Inguane, José Paulo Langa
RATIONALE: Mozambique has recorded cyclically epidemic outbreaks of cholera. Antibiotic therapy is recommended in specific situations for management and control of cholera outbreaks. However, an increase in resistance rates to antibiotics by Vibrio cholerae has been reported in several epidemic outbreaks worldwide. On the other hand, there are few recent records of continuous surveillance of antibiotics susceptibility pattern of V. cholerae in Mozambique. GOALS: The purpose of this study was to evaluate antibiotics resistance pattern of Vibrio cholerae O1 Ogawa isolated during Cholera outbreaks in Mozambique to commonly used antibiotics...
2017: PloS One
https://www.readbyqxmd.com/read/28780309/clinical-and-microbiological-characteristics-of-pantoea-agglomerans-infection-in-children
#8
Ayşe Büyükcam, Özlem Tuncer, Deniz Gür, Banu Sancak, Mehmet Ceyhan, Ali Bülent Cengiz, Ateş Kara
Pantoea agglomerans is an environmental Gram-negative bacterium that rarely is responsible for the infections in humans but it is often a causative factor of a number of occupational diseases. This study evaluated the clinical and microbiological characteristics and pathogenicity of P. agglomerans in children. We retrospectively reviewed microbiological test results for all children (1 month old to 18 years old) who were admitted to our pediatric hospital between January 2000 to June 2015 and had positive clinical specimen cultures for P...
August 2, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28780098/prevalence-and-characterization-of-methicillin-resistant-staphylococcus-aureus-carrying-meca-or-mecc-and-methicillin-susceptible-staphylococcus-aureus-in-dairy-sheep-farms-in-central-italy
#9
G Giacinti, V Carfora, A Caprioli, D Sagrafoli, N Marri, G Giangolini, R Amoruso, M Iurescia, F Stravino, S Dottarelli, F Feltrin, A Franco, S Amatiste, A Battisti
Between January and May 2012, a total of 286 bulk tank milk samples from dairy sheep farms located in central Italy were tested for the presence of Staphylococcus aureus. One hundred fifty-three samples were positive for S. aureus (53.5%), with an average count of 2.53 log cfu/mL. A total of 679 S. aureus colonies were screened for methicillin resistance by the cefoxitin disk diffusion test, and 104 selected cefoxitin-susceptible isolates were also tested for their susceptibility to other antimicrobials representative of the most relevant classes active against Staphylococcus spp...
August 2, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28764160/revisiting-nitrofurantoin-for-vancomycin-resistant-enterococci
#10
Suneeta Meena, Sarita Mohapatra, Seema Sood, Benu Dhawan, Bimal Ku Das, Arti Kapil
INTRODUCTION: Enterococcal infection has emerged as a major therapeutic challenge. Emergence of High Level Aminoglycoside Resistance (HLAR) and Vancomycin-Resistant Enterococcus (VRE) has further limited the drug therapy in enterococcal infections. However, nitrofuratoin being an old drug reported to have less resistance in comparison to the other classes of antimicrobial agents. AIM: To detect susceptibility of nitrofurantoin against VRE isolates from Urinary Tract Infection (UTI) of outdoor and indoor patient departments...
June 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28763905/-analysis-of-drug-resistance-of-acinetobacter-baumannii-in-wound-of-children-with-traffic-injury-and-its-relationship-with-antibiotic-use
#11
S Liu, C Wang, Y X Fu
Objective: To know the drug resistance of Acinetobacter baumannii (AB) in wound of children with traffic injury and its relationship with antibiotic use. Methods: Wound exudate of 226 children with traffic injury admitted to our unit from January 2010 to December 2015 were collected. API bacteria identification panels and fully automatic microbiological identification system were used to identify pathogens. Kirby-Bauer paper disk diffusion method was used to detect the drug resistance of pathogens to 18 antibiotics including amoxycillin/clavulanic acid, piperacillin/tazobactam, and imipenem...
July 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28762368/antibiotic-susceptibility-profiles-of-non-fermenting-gram-negative-bacilli-at-a-tertiary-care-hospital-in-patiala-india
#12
Udhayvir Singh Grewal, Rupinder Bakshi, Geeta Walia, Parth Rajeshbhai Shah
BACKGROUND: Non-fermenting Gram-negative bacilli (NFGNB) have emerged as a major cause of healthcare-associated infections and are innately resistant to many antibiotics. AIM: The aim of this study was to determine the prevalence of NFGNB isolated from various clinical specimens and evaluate their antimicrobial susceptibility profiles. MATERIALS AND METHODS: This retrospective study was done at our Department of Microbiology from December 2015 to December 2016...
April 2017: Nigerian Postgraduate Medical Journal
https://www.readbyqxmd.com/read/28758109/dissemination-and-molecular-characterization-of-staphylococcus-aureus-at-a-tertiary-referral-hospital-in-xiamen-city-china
#13
Yiwen Yu, Yihui Yao, Qinyun Weng, Jingyi Li, Jianwei Huang, Yiqun Liao, Fu Zhu, Qifeng Zhao, Xu Shen, Jianjun Niu
Staphylococcus aureus is a global epidemic pathogen that causes heavy disease burden. The aim of this study was to determine which globally known S. aureus lineages are currently present in a hospital of Xiamen. Therefore, the 426 S. aureus strains were detected by Melting Curve Analysis (MCA) and genotyped by Pulsed Field Gel Electrophoresis (PFGE) as well as Multicolor Melting Curve Analysis-Based Multilocus Melt Typing (MLMT). In addition, Multilocus Sequence Typing (MLST) was used to identify 108 representative strains...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28745287/copper-nanoflowers-as-effective-antifungal-agents-for-plant-pathogenic-fungi
#14
Avinash P Ingle, Mahendra Rai
Flower-shaped copper nanoparticles were synthesised by a green and ecofriendly chemical reduction approach using copper sulphate and cytyltrimethal ammonium bromide. The UV-vis spectrophotometer analysis showed maximum absorption at about 552 nm, which is specifically reported for copper nanoparticles. The crystal lattice structure of copper nanoflowers was confirmed by X-ray diffraction analysis. Further, the transmission electron microscopic studies revealed the flower shape copper nanoparticles in the size range of 100-500 nm...
August 2017: IET Nanobiotechnology
https://www.readbyqxmd.com/read/28743282/a-hospital-based-surveillance-of-metallo-beta-lactamase-producing-gram-negative-bacteria-in-nepal-by-imipenem-edta-disk-method
#15
Pratigya Thapa, Dinesh Bhandari, Dhiraj Shrestha, Hiramani Parajuli, Prakash Chaudhary, Jyoti Amatya, Ritu Amatya
BACKGROUND: A rising threat of the rapid spread of acquired metallo-beta-lactamases (MBLs) among major Gram-negative pathogens is a matter of public health concern worldwide. Hence, for a low income nation like Nepal, surveillance data on MBL producing clinical isolates via a cost effective technique is necessary to prevent their dissemination as well as formulation and regulation of antimicrobial stewardship policy. METHODS: The prospective study was conducted at Nepal Medical College, Kathmandu from May to October, 2014 to assess the prevalence of MBL production among ceftazidime-resistant Gram-negative rods (GNRs) isolates...
July 25, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28742000/high-prevalence-of-multidrug-resistant-escherichia-coli-isolates-from-children-with-and-without-diarrhoea-and-their-susceptibility-to-the-antibacterial-activity-of-extracts-fractions-of-fruits-native-to-mexico
#16
Magdalena de Jesús Uribe-Beltrán, Yesmi Patricia Ahumada-Santos, Sylvia Páz Díaz-Camacho, Carlos Alberto Eslava-Campos, Jesús Ernesto Reyes-Valenzuela, María Elena Báez-Flores, Ignacio Osuna-Ramírez, Francisco Delgado-Vargas
PURPOSE: This paper aims to evaluate the antimicrobial resistance of Esherichia coli isolates from children under 5 years old, with and without diarrhoea, who were hospital outpatients in Culiacan, Sinaloa, Mexico. It also looks at the antimicrobial activity of fruit extracts against selected multidrug-resistant (MDR) E. coli strains. METHODOLOGY: A total of 205 E. coli isolates from stool samples were collected from 94 children under 5 years old who were outpatients from two hospitals in the city of Culiacan, Sinaloa, Mexico, during the autumn/winter of 2003/04; their resistance profiles to 19 commercial antimicrobials were investigated using the Kirby-Bauer method...
July 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28735317/genetic-characterization-of-a-novel-composite-transposon-carrying-arma-and-aac-6-ib-genes-in-an-escherichia-coli-isolate-from-egypt
#17
Mona T Kashef, Omneya M Helmy
Aminoglycosides are used in treating a wide range of infections caused by Gram-positive and Gram-negative bacteria; however, aminoglycoside resistance is common and occurs by several mechanisms. Among these mechanisms is bacterial rRNA methylation by the 16S rRNA methyl transferase (16S-RMTase) enzymes; but data about the spread of this mechanism in Egypt are scarce. Cephalosporins are the most commonly used antimicrobial agents in Egypt; therefore, this study was conducted to determine the frequency of 16S-RMTase among third generation cephalosporin-resistant clinical isolates in Egypt...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28729206/antimicrobial-resistance-patterns-of-gram-negative-bacteria-isolated-from-bloodstream-infections-in-an-iranian-referral-pediatric-hospital-a-six-year-study
#18
Shima Mahmoudi, Masoumeh Mahzari, Maryam Banar, Babak Pourakbari, Mohammad Taghi Haghi Ashtiani, Mohsen Mohammadi, Sepideh Keshavarz Valian, Setareh Mamishi
INTRODUCTION: Bloodstream infections (BSIs) are considered as a major cause of morbidity and mortality in children all over the world. The aim of this study was to describe the epidemiology and antimicrobial resistance patterns of Gram-negative bacteria (GNB) isolated from bloodstream infections (BSIs) in children admitted to the Children Medical Center Hospital (CMC), Tehran, Iran. METHODS: Clinical and microbiological data of the patients with positive blood cultures was collected from March 2011 to September 2016...
July 17, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28719657/isolation-and-in-vitro-evaluation-of-bacteriophages-against-mdr-bacterial-isolates-from-septic-wound-infections
#19
Roja Rani Pallavali, Vijaya Lakshmi Degati, Dakshayani Lomada, Madhava C Reddy, Vijaya Raghava Prasad Durbaka
Multi-drug resistance has become a major problem for the treatment of pathogenic bacterial infections. The use of bacteriophages is an attractive approach to overcome the problem of drug resistance in several pathogens that cause fatal diseases. Our study aimed to isolate multi drug resistant bacteria from patients with septic wounds and then isolate and apply bacteriophages in vitro as alternative therapeutic agents. Pus samples were aseptically collected from Rajiv Gandhi Institute of Medical Science (RIMS), Kadapa, A...
2017: PloS One
https://www.readbyqxmd.com/read/28717851/multidrug-resistant-salmonellae-isolated-in-japanese-quails-reared-in-abeokuta-nigeria
#20
Ezekiel O Omoshaba, F O Olufemi, O E Ojo, A O Sonibare, M Agbaje
Salmonellosis is a major bacterial disease causing huge economic losses in the poultry industry worldwide. This study was carried out to determine the period prevalence and antimicrobial susceptibility of Salmonella enterica in Japanese quails in Abeokuta, Nigeria. Four hundred cloacal swabs of quail birds were collected from 4 locations within Abeokuta. Salmonella was isolated from the samples using conventional methods for selective isolation of Salmonella and biochemical identification. Isolates were confirmed by polymerase chain reaction assays for the amplification and detection of Salmonella-associated virulence genes (invA and stn) using specific primers...
July 17, 2017: Tropical Animal Health and Production
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