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https://www.readbyqxmd.com/read/29674148/seasonal-differences-in-trace-element-concentrations-and-distribution-in-spartina-alterniflora-root-tissue
#1
Huan Feng, Yu Qian, J Kirk Cochran, Qingzhi Zhu, Christina Heilbrun, Li Li, Wen Hu, Hanfei Yan, Xiaojing Huang, Mingyuan Ge, Evgeny Nazareski, Yong S Chu, Shinjae Yoo, Xuebin Zhang, Chang-Jun Liu
The present study uses nanometer-scale synchrotron X-ray nanofluorescence to investigate season differences in concentrations and distributions of major (Ca, K, S and P) and trace elements (As, Cr, Cu, Fe and Zn) in the root system of Spartina alterniflora collected from Jamaica Bay, New York, in April and September 2015. The root samples were cross-sectioned at a thickness of 10 μm. Selected areas in the root epidermis and endodermis were mapped with a sampling resolution of 100 and 200 nm, varying with the mapping areas...
April 13, 2018: Chemosphere
https://www.readbyqxmd.com/read/29660873/determining-the-speciation-of-zn-in-soils-around-the-sediment-ponds-of-chemical-plants-by-xrd-and-xafs-spectroscopy-and-sequential-extraction
#2
Tatiana Minkina, Dina Nevidomskaya, Tatiana Bauer, Victoria Shuvaeva, Alexander Soldatov, Saglara Mandzhieva, Yan Zubavichus, Alexander Trigub
For a correct assessment of risk of polluted soil, it is crucial to establish the speciation and mobility of the contaminants. The aim of this study was to investigate the speciation and transformation of Zn in strongly technogenically transformed contaminated Spolic Technosols for a long time in territory of sludge collectors by combining analytical techniques and synchrotron techniques. Sequential fractionation of Zn compounds in studied soils revealed increasing metal mobility. Phyllosilicates and Fe and Mn hydroxides were the main stabilizers of Zn mobility...
April 12, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29629368/tracking-of-short-distance-transport-pathways-in-biological-tissues-by-ultra-small-nanoparticles
#3
Jana S Segmehl, Alessandro Lauria, Tobias Keplinger, John K Berg, Ingo Burgert
In this work, ultra-small europium-doped HfO2 nanoparticles were infiltrated into native wood and used as trackers for studying penetrability and diffusion pathways in the hierarchical wood structure. The high electron density, laser induced luminescence, and crystallinity of these particles allowed for a complementary detection of the particles in the cellular tissue. Confocal Raman microscopy and high-resolution synchrotron scanning wide-angle X-ray scattering (WAXS) measurements were used to detect the infiltrated particles in the native wood cell walls...
2018: Frontiers in Chemistry
https://www.readbyqxmd.com/read/29515611/synchrotron-time-lapse-imaging-of-lignocellulosic-biomass-hydrolysis-tracking-enzyme-localization-by-protein-autofluorescence-and-biochemical-modification-of-cell-walls-by-microfluidic-infrared-microspectroscopy
#4
Marie-Françoise Devaux, Frédéric Jamme, William André, Brigitte Bouchet, Camille Alvarado, Sylvie Durand, Paul Robert, Luc Saulnier, Estelle Bonnin, Fabienne Guillon
Tracking enzyme localization and following the local biochemical modification of the substrate should help explain the recalcitrance of lignocellulosic plant cell walls to enzymatic degradation. Time-lapse studies using conventional imaging require enzyme labeling and following the biochemical modifications of biopolymers found in plant cell walls, which cannot be easily achieved. In the present work, synchrotron facilities have been used to image the enzymatic degradation of lignocellulosic biomass without labeling the enzyme or the cell walls...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29514247/absorption-of-foliar-applied-zn-fertilizers-by-trichomes-in-soybean-and-tomato
#5
Cui Li, Peng Wang, Enzo Lombi, Miaomiao Cheng, Caixian Tang, Daryl L Howard, Neal W Menzies, Peter M Kopittke
The present study investigated the role of trichomes in absorption of foliar-applied zinc fertilizers in soybean and tomato. Using synchrotron-based X-ray fluorescence microscopy for in situ analyses of hydrated leaves, we found that upon foliar application of ZnSO4, Zn accumulated within 15 min in some non-glandular trichomes in soybean, but not in tomato. However, analyses of cross-sections of soybean leaves did not show any marked accumulation of Zn in tissues surrounding trichomes. Furthermore, when near-isogenic lines of soybean differing 10-fold in trichome density were used to compare Zn absorption, it was found that foliar Zn absorption was not related to trichome density...
April 27, 2018: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29339081/disorder-to-order-transitions-in-the-molten-globule-like-golgi-reassembly-and-stacking-protein
#6
Luís F S Mendes, Luis G M Basso, Patricia S Kumagai, Raquel Fonseca-Maldonado, Antonio J Costa-Filho
BACKGROUND: Golgi Reassembly and Stacking Proteins (GRASPs) are widely spread among eukaryotic cells (except plants) and are considered as key components in both the stacking of the Golgi cisternae and its lateral connection. Furthermore, GRASPs were also proved essential in the unconventional secretion pathway of several proteins, even though the mechanism remains obscure. It was previously observed that the GRASP homologue in Cryptococcus neoformans has a molten globule-like behavior in solution...
April 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29303173/thermodynamics-and-performance-of-the-mg-h-f-system-for-thermochemical-energy-storage-applications
#7
Mariana S Tortoza, Terry D Humphries, Drew A Sheppard, Mark Paskevicius, Matthew R Rowles, M Veronica Sofianos, Kondo-Francois Aguey-Zinsou, Craig E Buckley
Magnesium hydride (MgH2 ) is a hydrogen storage material that operates at temperatures above 300 °C. Unfortunately, magnesium sintering occurs above 420 °C, inhibiting its application as a thermal energy storage material. In this study, the substitution of fluorine for hydrogen in MgH2 to form a range of Mg(Hx F1-x )2 (x = 1, 0.95, 0.85, 0.70, 0.50, 0) composites has been utilised to thermodynamically stabilise the material, so it can be used as a thermochemical energy storage material that can replace molten salts in concentrating solar thermal plants...
January 24, 2018: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29259205/action-of-lytic-polysaccharide-monooxygenase-on-plant-tissue-is-governed-by-cellular-type
#8
Brigitte Chabbert, Anouck Habrant, Mickaël Herbaut, Laurence Foulon, Véronique Aguié-Béghin, Sona Garajova, Sacha Grisel, Chloé Bennati-Granier, Isabelle Gimbert-Herpoël, Frédéric Jamme, Matthieu Réfrégiers, Christophe Sandt, Jean-Guy Berrin, Gabriel Paës
Lignocellulosic biomass bioconversion is hampered by the structural and chemical complexity of the network created by cellulose, hemicellulose and lignin. Biological conversion of lignocellulose involves synergistic action of a large array of enzymes including the recently discovered lytic polysaccharide monooxygenases (LPMOs) that perform oxidative cleavage of cellulose. Using in situ imaging by synchrotron UV fluorescence, we have shown that the addition of AA9 LPMO (from Podospora anserina) to cellulases cocktail improves the progression of enzymes in delignified Miscanthus x giganteus as observed at tissular levels...
December 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29202288/role-of-chelant-on-cu-distribution-and-speciation-in-lolium-multiflorum-by-synchrotron-techniques
#9
Yan-Ping Zhao, Jin-Li Cui, Ting-Shan Chan, Jun-Cai Dong, Dong-Liang Chen, Xiang-Dong Li
Chelants are known to enhance metal translocation in plants; however, the underlying mechanisms are still not fully understood. This study aimed to elucidate the distribution and speciation of Cu in ryegrass (Lolium multiflorum) in both absence and presence of the biodegradable chelant [S,S']-ethylenediamine disuccinic acid (EDDS). The results showed that EDDS increased the Cu translocation factor from root to shoot by 6-9 folds under CuEDDS in comparison with free Cu (50-250μM). Synchrotron-based microscopic X-ray fluorescence (μ-XRF) mapping revealed that EDDS alleviated Cu deposition in the root meristem of root apex and the junction of lateral root zone, and facilitated Cu transport to root stele for subsequent translocation upwards...
April 15, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29168918/nano-biohybrids-in-vivo-synthesis-of-metal-organic-frameworks-inside-living-plants
#10
Joseph J Richardson, Kang Liang
Plants have a complex passive fluid transport system capable of internalizing small molecules from the environment, and this system offers an ideal route for augmenting plants with functional nanomaterials. Current plant augmentation techniques use pre-formed nanomaterials and permeabilizing agents or plant cuttings. A so far unexplored concept is the formation of the functional material, in situ, from precursors small enough to be passively internalized through the roots without harming the plants. Metal-organic frameworks are ideal for in situ synthesis as they are composed of metal ions coordinated with organic ligands and have recently been mineralized around single-celled organisms in mild aqueous conditions...
November 23, 2017: Small
https://www.readbyqxmd.com/read/29118113/measurement-of-micro-scale-soil-deformation-around-roots-using-four-dimensional-synchrotron-tomography-and-image-correlation
#11
S D Keyes, L Cooper, S Duncan, N Koebernick, D M McKay Fletcher, C P Scotson, A van Veelen, I Sinclair, T Roose
This study applied time lapse (four-dimensional) synchrotron X-ray computed tomography to observe micro-scale interactions between plant roots and soil. Functionally contrasting maize root tips were repeatedly imaged during ingress into soil columns of varying water content and compaction. This yielded sequences of three-dimensional densiometric data, representing time-resolved geometric soil and root configurations at the micronmetre scale. These data were used as inputs for two full-field kinematic quantification methods, which enabled the analysis of three-dimensional soil deformation around elongating roots...
November 2017: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/29096153/uptake-and-speciation-of-uranium-in-synthetic-gypsum-caso-4-%C3%A2-2h-2-o-applications-to-radioactive-mine-tailings
#12
Jinru Lin, Wei Sun, Jacques Desmarais, Ning Chen, Renfei Feng, Patrick Zhang, Dien Li, Arthur Lieu, John S Tse, Yuanming Pan
Phosphogypsum formed from the production of phosphoric acid represents by far the biggest accumulation of gypsum-rich wastes in the world and commonly contains elevated radionuclides, including uranium, as well as other heavy metals and metalloids. Therefore, billions-of-tons of phosphogypsum stockpiled worldwide not only possess serious environmental problems but also represent a potential uranium resource. Gypsum is also a major solid constituent in many other types of radioactive mine tailings, which stems from the common usage of sulfuric acid in extraction processes...
January 2018: Journal of Environmental Radioactivity
https://www.readbyqxmd.com/read/29091286/manganese-distribution-and-speciation-help-to-explain-the-effects-of-silicate-and-phosphate-on-manganese-toxicity-in-four-crop-species
#13
F Pax C Blamey, Brigid A McKenna, Cui Li, Miaomiao Cheng, Caixian Tang, Haibo Jiang, Daryl L Howard, David J Paterson, Peter Kappen, Peng Wang, Neal W Menzies, Peter M Kopittke
Soil acidity and waterlogging increase manganese (Mn) in leaf tissues to potentially toxic concentrations, an effect reportedly alleviated by increased silicon (Si) and phosphorus (P) supply. Effects of Si and P on Mn toxicity were studied in four plant species using synchrotron-based micro X-ray fluorescence (μ-XRF) and nanoscale secondary ion mass spectrometry (NanoSIMS) to determine Mn distribution in leaf tissues and using synchrotron-based X-ray absorption spectroscopy (XAS) to measure Mn speciation in leaves, stems and roots...
February 2018: New Phytologist
https://www.readbyqxmd.com/read/29030805/an-explicit-structural-model-of-root-hair-and-soil-interactions-parameterised-by-synchrotron-x-ray-computed-tomography
#14
Samuel David Keyes, Konstantinos C Zygalakis, Tiina Roose
The rhizosphere is a zone of fundamental importance for understanding the dynamics of nutrient acquisition by plant roots. The canonical difficulty of experimentally investigating the rhizosphere led long ago to the adoption of mathematical models, the most sophisticated of which now incorporate explicit representations of root hairs and rhizosphere soil. Mathematical upscaling regimes, such as homogenisation, offer the possibility of incorporating into larger-scale models the important mechanistic processes occurring at the rhizosphere scale...
December 2017: Bulletin of Mathematical Biology
https://www.readbyqxmd.com/read/28935406/fate-and-chemical-speciation-of-antimony-sb-during-uptake-translocation-and-storage-by-rye-grass-using-xanes-spectroscopy
#15
Ying Ji, Géraldine Sarret, Rainer Schulin, Susan Tandy
Antimony (Sb) is a contaminant of increased prevalence in the environment, but there is little knowledge about the mechanisms of its uptake and translocation within plants. Here, we applied for the synchrotron based X-ray absorption near-edge structure (XANES) spectroscopy to analyze the speciation of Sb in roots and shoots of rye grass (Lolium perenne L. Calibra). Seedlings were grown in nutrient solutions to which either antimonite (Sb(III)), antimonate (Sb(V)) or trimethyl-Sb(V) (TMSb) were added. While exposure to Sb(III) led to around 100 times higher Sb accumulation in the roots than the other two treatments, there was no difference in total Sb in the shoots...
December 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28923732/ammonium-based-fertilizers-enhance-cd-accumulation-in-carpobrotus-rossii-grown-in-two-soils-differing-in-ph
#16
Miaomiao Cheng, Anan Wang, Caixian Tang
Nitrogen fertilization has been shown to improve Cd uptake by plants but there is little information on the effect of N form. This study examined the effects of N form on Cd bioavailability and phytoextraction in two soils differing in pH. Plants of halophytic species Carpobrotus rossii were grown in an acidic Sodosol [pH (CaCl2 ) 4.9] and a neutral Vertosol (pH 7.2) spiked with 20 mg kg-1 Cd as CdCl2 . Three N forms, KNO3 , (NH4 )2 SO4 and (NH2 )2 CO at a rate of 24 mg N kg-1 were applied at weekly intervals, together with nitrification inhibitor dicyanodiamide...
December 2017: Chemosphere
https://www.readbyqxmd.com/read/28918933/structural-proteomics-topology-and-relative-accessibility-of-plant-lipid-droplet-associated-proteins
#17
Pascale Jolivet, Laure Aymé, Alexandre Giuliani, Frank Wien, Thierry Chardot, Yann Gohon
Lipid droplets are the major stock of lipids in oleaginous plant seeds. Despite their economic importance for oil production and biotechnological issues (biofuels, lubricants and plasticizers), numerous questions about their formation, structure and regulation are still unresolved. To determine water accessible domains of protein coating at lipid droplets surface, a structural proteomic approach has been performed. This technique relies on the millisecond timescale production of hydroxyl radicals by the radiolysis of water using Synchrotron X-ray white beam...
October 3, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28857201/wheat-flag-leaf-epicuticular-wax-morphology-and-composition-in-response-to-moderate-drought-stress-are-revealed-by-sem-ftir-atr-and-synchrotron-x-ray-spectroscopy
#18
Ian R Willick, Rachid Lahlali, Perumal Vijayan, David Muir, Chithra Karunakaran, Karen K Tanino
Wheat (Triticum aestivum L.) is the largest cereal crop grown in Western Canada where drought during late vegetative and seed filling stages affects plant development and yield. To identify new physiochemical markers associated with drought tolerance, epidermal characteristics of the flag leaf of two wheat cultivars with contrasting drought tolerance were investigated. The drought resistant 'Stettler' had a lower drought susceptibility index, greater harvest index and water-use efficiency than the susceptible 'Superb'...
March 2018: Physiologia Plantarum
https://www.readbyqxmd.com/read/28758681/high-resolution-synchrotron-imaging-shows-that-root-hairs-influence-rhizosphere-soil-structure-formation
#19
Nicolai Koebernick, Keith R Daly, Samuel D Keyes, Timothy S George, Lawrie K Brown, Annette Raffan, Laura J Cooper, Muhammad Naveed, Anthony G Bengough, Ian Sinclair, Paul D Hallett, Tiina Roose
In this paper, we provide direct evidence of the importance of root hairs on pore structure development at the root-soil interface during the early stage of crop establishment. This was achieved by use of high-resolution (c. 5 μm) synchrotron radiation computed tomography (SRCT) to visualise both the structure of root hairs and the soil pore structure in plant-soil microcosms. Two contrasting genotypes of barley (Hordeum vulgare), with and without root hairs, were grown for 8 d in microcosms packed with sandy loam soil at 1...
October 2017: New Phytologist
https://www.readbyqxmd.com/read/28710386/seed-coat-thinning-during-horsegram-macrotyloma-uniflorum-domestication-documented-through-synchrotron-tomography-of-archaeological-seeds
#20
Charlene Murphy, Dorian Q Fuller
Reduction of seed dormancy mechanisms, allowing for rapid germination after planting, is a recurrent trait in domesticated plants, and can often be linked to changes in seed coat structure, in particular thinning. We report evidence for seed coat thinning between 2,000 BC and 1,200 BC, in southern Indian archaeological horsegram (Macrotyloma uniflorum), which it has been possible to document with high precision and non-destructively, through high resolution x-ray computed tomography using a synchrotron...
July 14, 2017: Scientific Reports
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