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https://www.readbyqxmd.com/read/28432730/assessment-of-voluntary-deep-inspiration-breath-hold-with-cine-imaging-for-breast-radiotherapy
#1
Reuben Patrick Estoesta, Lani Attwood, Diana Naehrig, Elizabeth Claridge-Mackonis, David Odgers, Darren Martin, Melissa Pham, Joanne Toohey, Susan Carroll
Deep Inspiration Breath-Hold (DIBH) techniques for breast cancer radiation therapy (RT) have reduced cardiac dose compared to Free Breathing (FB). Recently, a voluntary deep inspiration breath-hold (vDIBH) technique was established using in-room lasers and skin tattoos to monitor breath-hold. An in-house quality assessment of positional reproducibility during RT delivery with vDIBH in patients with left-sided breast cancer was evaluated. The electronic portal imaging device (EPID) was used in cinematographic (CINE) mode to capture a sequence of images during beam delivery...
April 22, 2017: Journal of Medical Imaging and Radiation Oncology
https://www.readbyqxmd.com/read/28430125/erratum-tingting-lian-et-al-identification-of-site-specific-stroke-biomarker-candidates-by-laser-capture-microdissection-and-labeled-reference-peptide-int-j-mol-sci-2015-16-13427-13441
#2
https://www.readbyqxmd.com/read/28419375/transcriptional-profiling-of-cholinergic-neurons-from-basal-forebrain-identifies-changes-in-expression-of-genes-between-sleep-and-wake-mrna-profiling-in-basal-forebrain-with-sleep-sleep-deprivation
#3
Elena V Nikonova, Jason DA Gilliland, Keith Q Tanis, Alexei A Podtelezhnikov, Alison M Rigby, Raymond J Galante, Eva M Finney, David J Stone, John J Renger, Allan I Pack, Christopher J Winrow
Study objective: To assess differences in gene expression in cholinergic basal forebrain cells between sleeping and sleep deprived mice sacrificed at the same time of day. Methods: Tg(ChAT-eGFP)86Gsat mice expressing eGFP under control of the choline acetyltransferase (Chat) promoter were utilized to guide laser capture of cholinergic cells in basal forebrain. mRNA expression levels in these cells were profiled using microarrays. Gene expression in eGFP(+) neurons was compared (1) to that in eGFP(-) neurons and to adjacent white matter, (2) between 7:00 am (lights on) and 7:00 pm (lights off), (3) between sleep deprived and sleeping animals at 0, 3, 6, 9 hrs from lights on...
April 13, 2017: Sleep
https://www.readbyqxmd.com/read/28415711/mutational-analysis-of-multiple-lung-cancers-discrimination-between-primary-and-metastatic-lung-cancers-by-genomic-profile
#4
Taichiro Goto, Yosuke Hirotsu, Hitoshi Mochizuki, Takahiro Nakagomi, Daichi Shikata, Yujiro Yokoyama, Toshio Oyama, Kenji Amemiya, Kenichiro Okimoto, Masao Omata
In cases of multiple lung cancers, individual tumors may represent either a primary lung cancer or both primary and metastatic lung cancers. Treatment selection varies depending on such features, and this discrimination is critically important in predicting prognosis. The present study was undertaken to determine the efficacy and validity of mutation analysis as a means of determining whether multiple lung cancers are primary or metastatic in nature. The study involved 12 patients who underwent surgery in our department for multiple lung cancers between July 2014 and March 2016...
March 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/28413868/goniosynechialysis-and-repositioning-of-intraocular-lens-in-a-case-of-secondary-angle-closure-glaucoma
#5
Masaru Fujita, Masafumi Ogata, Yasuyuki Suzuki, Tsunetomo Ichihashi, Miyuki Nagahara, Kenji Kawai
PURPOSE: Implanting intraocular lens (IOL) in proper direction is important in cataract surgery to reduce the possibility of undesirable complications. We experienced a case who underwent vitrectomy combined with cataract surgery and developed secondary angle closure glaucoma caused by IOL misdirection. Goniosynechialysis (GSL) and repositioning of IOL successfully ameliorated the high intraocular pressure (IOP). CASE REPORT: A 64-year-old male with massive vitreous hemorrhage underwent vitrectomy combined with cataract surgery...
April 20, 2017: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/28413809/preclinical-imaging-of-iridocorneal-angle-and-fundus-using-a-modified-integrated-flexible-handheld-probe
#6
Xun Jie Jeesmond Hong, Vengalathunadakal K Shinoj, Vadakke Matham Murukeshan, Mani Baskaran, Tin Aung
A flexible handheld imaging probe consisting of a [Formula: see text] charge-coupled device camera, light-emitting diode light sources, and near-infrared laser source is designed and developed. The imaging probe is designed with specifications to capture the iridocorneal angle images and posterior segment images. Light propagation from the anterior chamber of the eye to the exterior is considered analytically using Snell's law. Imaging of the iridocorneal angle region and fundus is performed on ex vivo porcine samples and subsequently on small laboratory animals, such as the New Zealand white rabbit and nonhuman primate, in vivo...
April 2017: Journal of Medical Imaging
https://www.readbyqxmd.com/read/28411696/first-10-month-results-of-the-vascular-quality-initiative-varicose-vein-registry
#7
Andrea T Obi, Danielle C Sutzko, Jose I Almeida, Lowell Kabnick, Jack L Cronenwett, Nicholas H Osborne, Brajesh K Lal, Thomas W Wakefield
OBJECTIVE: The Vascular Quality Initiative Varicose Vein Registry (VQI VVR) represents a new Patient Safety Organization database launched in January 2015 as a collaborative effort between the American Venous Forum and the Society for Vascular Surgery. This study was undertaken to identify real-world trends among treatment choices and outcomes of varicose vein patients. METHODS: Registry data prospectively captured anatomic, procedural, and outcome data for patients with C2 or more severe disease undergoing intervention for venous varicosities from January to November 2015...
May 2017: Journal of Vascular Surgery. Venous and Lymphatic Disorders
https://www.readbyqxmd.com/read/28406494/laser-capture-microdissection-of-pancreatic-acinar-cells-to-identify-proteomic-alterations-in-a-murine-model-of-caerulein-induced-pancreatitis
#8
John P Shapiro, Hannah M Komar, Baris Hancioglu, Lianbo Yu, Ming Jin, Yuko Ogata, Phil A Hart, Zobeida Cruz-Monserrate, Gregory B Lesinski, Darwin L Conwell
OBJECTIVES: Chronic pancreatitis (CP) is characterized by inflammation and fibrosis of the pancreas, leading to pain, parenchymal damage, and loss of exocrine and endocrine function. There are currently no curative therapies; diagnosis remains difficult and aspects of pathogenesis remain unclear. Thus, there is a need to identify novel biomarkers to improve diagnosis and understand pathophysiology. We hypothesize that pancreatic acinar regions contain proteomic signatures relevant to disease processes, including secreted proteins that could be detected in biofluids...
April 13, 2017: Clinical and Translational Gastroenterology
https://www.readbyqxmd.com/read/28405177/mapping-mitochondrial-heteroplasmy-in-a-leydig-tumor-by-laser-capture-micro-dissection-and-cycling-temperature-capillary-electrophoresis
#9
Paulo Refinetti, Christian Arstad, William G Thilly, Stephan Morgenthaler, Per Olaf Ekstrøm
BACKGROUND: The growth of tumor cells is accompanied by mutations in nuclear and mitochondrial genomes creating marked genetic heterogeneity. Tumors also contain non-tumor cells of various origins. An observed somatic mitochondrial mutation would have occurred in a founding cell and spread through cell division. Micro-anatomical dissection of a tumor coupled with assays for mitochondrial point mutations permits new insights into this growth process. More generally, the ability to detect and trace, at a histological level, somatic mitochondrial mutations in human tissues and tumors, makes these mutations into markers for lineage tracing...
2017: BMC Clinical Pathology
https://www.readbyqxmd.com/read/28399039/characterization-of-corneal-involvement-in-eyes-with-mucous-membrane-pemphigoid-by-in-vivo-confocal-microscopy
#10
Tudor C Tepelus, Jianyan Huang, SriniVas R Sadda, Olivia L Lee
PURPOSE: To describe the morphological features of the corneal epithelial layers, subbasal nerve plexus, stroma, and endothelium in patients with mucous membrane pemphigoid (MMP) as shown by in vivo confocal microscopy (IVCM). METHODS: Central corneal images were captured from 10 healthy age-matched control eyes and 30 eyes with clinically diagnosed MMP by in vivo laser scanning confocal microscopy (HRT III RCM). Morphological changes of the corneal epithelial layers, stroma, and endothelium, characteristics of corneal nerves, and presence of inflammatory dendritic cells (DCs) were evaluated...
April 10, 2017: Cornea
https://www.readbyqxmd.com/read/28397751/in-vitro-studies-on-a-microfluidic-sensor-with-embedded-obstacles-using-new-antibacterial-synthetic-compounds-1-tdppo-mixed-prop-2-en-1-one-with-difluoro-phenyl
#11
Changhyun Roh, Jaewoong Lee, Mayank Kinger, Chankyu Kang
This paper describes the use of an analytical microfluidic sensor for accelerating chemo-repellent response and strong anti-bacterial 1-(Thien-2-yl)-3-(2, 6-difluoro phenyl) prop-2-en-1-one (1-TDPPO). The chemically-synthesized antimicrobial agent, which included prop-2-en-1-one and difluoro phenyl groups, was moving through an optically transparent polydimethylsiloxane (PDMS) microfluidic sensor with circular obstacles arranged evenly. The response, growth and distribution of fluorescent labeling Pseudomonas aeruginosa PAO1 against the antimicrobial agent were monitored by confocal laser scanning microscope (CLSM)...
April 8, 2017: Sensors
https://www.readbyqxmd.com/read/28395300/noninvasive-quantification-of-retinal-microglia-using-widefield-autofluorescence-imaging
#12
Despina Kokona, Nadia Schneider, Helena Giannakaki-Zimmermann, Joel Jovanovic, Andreas Ebneter, Martin Zinkernagel
Purpose: To validate widefield autofluorescence (AF) in vivo imaging of the retina in mice expressing green fluorescent protein (gfp) in microglia, and to monitor retinal microglia reconstitution in vivo after lethal irradiation and bone marrow transplantation. Methods: Transgenic Cx3cr1gfp/gfp and wildtype Balb/c mice were used in this study. A confocal scanning laser ophthalmoscope was used for AF imaging with a 55° and a widefield 102° lens. Intrasession reproducibility was assessed for each lens...
April 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28391846/gold-nanoparticles-modified-porous-silicon-chip-for-saldi-ms-determination-of-glutathione-in-cells
#13
Jing Wang, Mingsha Jie, Haifang Li, Luyao Lin, Ziyi He, Shiqi Wang, Jin-Ming Lin
The gold nanoparticles (Au NPs) modified porous silicon chip based surface assisted laser desorption/ionization mass spectrometry (SALDI-MS) was developed to capture and analyze glutathione (GSH) in cells. With silver-assisted chemical etching, Ag nanoparticles (Ag NPs) were generated and deposited on the silicon surface and the nanopores were etched on silicon substrate. Then Au NPs were in-situ synthesized on the ridges of silicon nanopores. This Ag-Au NPs modified porous silicon surface could specially capture and enrich thiol compounds through Au-S binding, and it could also function as matrix to assist ionization for SALDI-MS...
June 1, 2017: Talanta
https://www.readbyqxmd.com/read/28391630/fundus-imaging-in-newborn-children-with-wide-field-scanning-laser-ophthalmoscope
#14
Vigdis Magnusdottir, Wouter B Vehmeijer, Thorunn S Eliasdottir, Sveinn H Hardarson, Nicoline E Schalij-Delfos, Einar Stefánsson
PURPOSE: Current fundus imaging in newborn babies requires mydriatics, eye specula and corneal contact. We propose that a scanning laser ophthalmoscope (SLO) allows ultra wide-field imaging with reduced stress for the child. METHODS: This prospective observational single centre study was conducted in Landspítali, University Hospital, Reykjavik, Iceland. In this study, a noncontact wide-field SLO (Optomap 200Tx) was used to image the retina in healthy full-term newborns without the use of mydriatics or eye specula...
April 9, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/28384252/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-the-identification-of-burkholderia-pseudomallei-from-asia-and-australia-and-differentiation-between-burkholderia-species
#15
Vichaya Suttisunhakul, Apinya Pumpuang, Peeraya Ekchariyawat, Vanaporn Wuthiekanun, Mindy G Elrod, Paul Turner, Bart J Currie, Rattanaphone Phetsouvanh, David A B Dance, Direk Limmathurotsakul, Sharon J Peacock, Narisara Chantratita
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is increasingly used for rapid bacterial identification. Studies of Burkholderia pseudomallei identification have involved small isolate numbers drawn from a restricted geographic region. There is a need to expand the reference database and evaluate B. pseudomallei from a wider geographic distribution that more fully captures the extensive genetic diversity of this species. Here, we describe the evaluation of over 650 isolates...
2017: PloS One
https://www.readbyqxmd.com/read/28380921/high-efficiency-fabrication-of-complex-microtube-arrays-by-scanning-focused-femtosecond-laser-bessel-beam-for-trapping-releasing-biological-cells
#16
Liang Yang, Shengyun Ji, Kenan Xie, Wenqiang Du, Bingjie Liu, Yanlei Hu, Jiawen Li, Gang Zhao, Dong Wu, Wenhao Huang, Suling Liu, Hongyuan Jiang, Jiaru Chu
In this paper, we present a focused femtosecond laser Bessel beam scanning technique for the rapid fabrication of large-area 3D complex microtube arrays. The femtosecond laser beam is converted into several Bessel beams by two-dimensional phase modulation using a spatial light modulator. By scanning the focused Bessel beam along a designed route, microtubes with variable size and flexible geometry are rapidly fabricated by two-photon polymerization. The fabrication time is reduced by two orders of magnitude in comparison with conventional point-to-point scanning...
April 3, 2017: Optics Express
https://www.readbyqxmd.com/read/28380666/whole-transcriptome-profiling-of-the-human-hippocampus-suggests-an-involvement-of-the-kibra-rs17070145-polymorphism-in-differential-activation-of-the-mapk-signaling-pathway
#17
I S Piras, J Krate, I Schrauwen, J J Corneveaux, G E Serrano, L Sue, T G Beach, M J Huentelman
The rs17070145-T variant of the WWC1 gene, coding for the KIBRA protein, has been associated with both increased episodic memory performance and lowered risk for late onset Alzheimer's disease, although the mechanism behind this protective effect has not been completely elucidated. To achieve a better understanding of the pathways modulated by rs17070145 and its associated functional variant(s), we used laser capture microdissection (LCM) and RNA-sequencing to investigate the effect of rs17070145 genotypes on whole transcriptome expression in the human hippocampus (HP) of 22 neuropathologically normal individuals, with a specific focus on the dentate gyrus (DG) and at the pyramidal cells (PC) of CA1 and CA3 sub-regions...
April 5, 2017: Hippocampus
https://www.readbyqxmd.com/read/28377615/ultra-high-mass-resolution-maldi-imaging-mass-spectrometry-of-proteins-and-metabolites-in-a-mouse-model-of-glioblastoma
#18
M Dilillo, R Ait-Belkacem, C Esteve, D Pellegrini, S Nicolardi, M Costa, E Vannini, E L de Graaf, M Caleo, L A McDonnell
MALDI mass spectrometry imaging is able to simultaneously determine the spatial distribution of hundreds of molecules directly from tissue sections, without labeling and without prior knowledge. Ultra-high mass resolution measurements based on Fourier-transform mass spectrometry have been utilized to resolve isobaric lipids, metabolites and tryptic peptides. Here we demonstrate the potential of 15T MALDI-FTICR MSI for molecular pathology in a mouse model of high-grade glioma. The high mass accuracy and resolving power of high field FTICR MSI enabled tumor specific proteoforms, and tumor-specific proteins with overlapping and isobaric isotopic distributions to be clearly resolved...
April 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28375281/collimation-testing-using-deflectometry-in-conjunction-with-windowed-fourier-transform-analysis
#19
Jitendra Dhanotia, Vimal Bhatia, Shashi Prakash
In this paper, we demonstrate a simple automated procedure for the detection of collimation of an optical beam by incorporating the windowed Fourier fringe analysis technique into a deflectometric setup. The experimental arrangement consists of a deflectometry-based system in which light from a laser is expanded and passed through a collimating lens. The transmitted light illuminates a coarse sinusoidal grating. The grating image is directly captured through a charge-coupled device. Typical image patterns corresponding to "in-focus," "at-focus," and "out-of-focus" positions of an optical beam are recorded...
March 10, 2017: Applied Optics
https://www.readbyqxmd.com/read/28374608/long-wave-infrared-lwir-molecular-laser-induced-breakdown-spectroscopy-libs-emissions-of-thin-solid-explosive-powder-films-deposited-on-aluminum-substrates
#20
Clayton S-C Yang, Feng Jin, Sudhir B Trivedi, Ei E Brown, Uwe Hommerich, Ashish Tripathi, Alan C Samuels
Thin solid films made of high nitro (NO2)/nitrate (NO3) content explosives were deposited on sand-blasted aluminum substrates and then studied using a mercury-cadmium-telluride (MCT) linear array detection system that is capable of rapidly capturing a broad spectrum of atomic and molecular laser-induced breakdown spectroscopy (LIBS) emissions in the long-wave infrared region (LWIR; ∼5.6-10 µm). Despite the similarities of their chemical compositions and structures, thin films of three commonly used explosives (RDX, HMX, and PETN) studied in this work can be rapidly identified in the ambient air by their molecular LIBS emission signatures in the LWIR region...
April 2017: Applied Spectroscopy
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