keyword
MENU ▼
Read by QxMD icon Read
search

Laser capture

keyword
https://www.readbyqxmd.com/read/28911071/prediction-of-pathological-response-to-neoadjuvant-treatment-in-rectal-cancer-with-a-two-protein-immunohistochemical-score-derived-from-stromal-gene-profiling
#1
S Gonçalves-Ribeiro, R Sanz-Pamplona, A Vidal, X Sanjuan, N Guillen Díaz-Maroto, A Soriano, J Guardiola, N Albert, M Martínez-Villacampa, I López, C Santos, J Serra-Musach, R Salazar, G Capellà, A Villanueva, D G Molleví
Background: Preoperative chemoradiotherapy followed by surgical mesorectal resection is the standard of care for locally advanced rectal carcinomas. Yet, predicting that patients will respond to treatment remains an unmet clinical challenge. Experimental design: Using laser-capture microdissection we isolated RNA from stroma and tumour glands from prospective pre-treatment samples (n = 15). Transcriptomic profiles were obtained hybridising PrimeView Affymetrix arrays...
September 1, 2017: Annals of Oncology: Official Journal of the European Society for Medical Oncology
https://www.readbyqxmd.com/read/28903393/eb1-protein-alteration-characterizes-sporadic-but-not-ulcerative-colitis-associated-colorectal-cancer
#2
Timo Gemoll, Sophie L Kollbeck, Karl F Karstens, Gia G Hò, Sonja Hartwig, Sarah Strohkamp, Katharina Schillo, Christoph Thorns, Martina Oberländer, Kathrin Kalies, Stefan Lehr, Jens K Habermann
BACKGROUND: While carcinogenesis in Sporadic Colorectal Cancer (SCC) has been thoroughly studied, less is known about Ulcerative Colitis associated Colorectal Cancer (UCC). This study aimed to identify and validate differentially expressed proteins between clinical samples of SCC and UCC to elucidate new insights of UCC/SCC carcinogenesis and progression. RESULTS: Multiplex-fluorescence two-dimensional gel electrophoresis (2-D DIGE) and mass spectrometry identified 67 proteoforms representing 43 distinct proteins...
August 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28903323/the-pancreatic-tumor-microenvironment-drives-changes-in-mirna-expression-that-promote-cytokine-production-and-inhibit-migration-by-the-tumor-associated-stroma
#3
Song Han, David H Gonzalo, Michael Feely, Daniel Delitto, Kevin E Behrns, Mark Beveridge, DongYu Zhang, Ryan Thomas, Jose G Trevino, Thomas D Schmittgen, Steven J Hughes
The pancreatic adenocarcinoma (PDAC) microenvironment is largely comprised of fibrotic tumor associated stroma (TAS) that contributes to the lethal biology of PDAC. microRNA (miRNA) are small non-coding RNAs that regulate gene expression. We hypothesized that interactions between PDAC cells and TAS cells within the microenvironment modulate miRNA expression and thus, tumor biology. We observed that miR-205 and members of the miR-200 family (miR-200a, -200b, -200c, -141 and miR-429) were exclusively expressed in PDAC cells, consistent with an epithelial miRNA signature, while miR-145 and miR-199 family members (miR-199a and -199b) were solely expressed in TAS cells, consistent with a stromal miRNA signature...
August 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28888073/selective-decline-of-neurotrophin-and-neurotrophin-receptor-genes-within-ca1-pyramidal-neurons-and-hippocampus-proper-correlation-with-cognitive-performance-and-neuropathology-in-mild-cognitive-impairment-and-alzheimer-s-disease
#4
Stephen D Ginsberg, Michael H Malek-Ahmadi, Melissa J Alldred, Shaoli Che, Irina Elarova, Yinghua Chen, Freddy Jeanneteau, Thorsten M Kranz, Moses V Chao, Scott E Counts, Elliott J Mufson
Hippocampal CA1 pyramidal neurons, a major component of the medial temporal lobe memory circuit, are selectively vulnerable during the progression of Alzheimer's disease (AD). The cellular mechanism(s) underlying degeneration of these neurons and the relationship to cognitive performance remains largely undefined. Here, we profiled neurotrophin and neurotrophin receptor gene expression within microdissected CA1 neurons along with regional hippocampal dissections from subjects who died with a clinical diagnosis of no cognitive impairment (NCI), mild cognitive impairment (MCI), or AD using laser capture microdissection (LCM), custom-designed microarray analysis, and qPCR of CA1 subregional dissections...
September 9, 2017: Hippocampus
https://www.readbyqxmd.com/read/28887614/the-pro-fibrotic-connective-tissue-growth-factor-ctgf-ccn2-correlates-with-the-number-of-necrotic-regenerative-foci-in-dystrophic-muscle
#5
María Gabriela Morales, María José Acuña, Daniel Cabrera, Roel Goldschmeding, Enrique Brandan
Connective tissue growth factor (CTGF/CCN2) has strong inflammatory and profibrotic activities. Its expression is enhanced in skeletal muscular dystrophies such as Duchenne muscular dystrophy (DMD), a myopathy characterized by exacerbated inflammation and fibrosis. In dystrophic tissue, necrotic-regenerative foci, myofibroblasts, newly-regenerated muscle fibers and necrosis all occur simultaneously. To determine if CCN2 is involved in the appearance of the foci, we studied their presence and characteristics in mdx mice (DMD mouse model) compared to mdx mice hemizygous for CCN2 (mdx-Ccn2+/-)...
September 8, 2017: Journal of Cell Communication and Signaling
https://www.readbyqxmd.com/read/28884710/atmospheric-pulsed-laser-deposition-and-thermal-annealing-of-plasmonic-silver-nanoparticle-films
#6
Taj M Khan, Mubarak M Mujawar, Katarzyna E Siewerska, Anuj Pokle, Tony Donnelly, Niall McEvoy, Georg S Duesberg, James G Lunney
A new method for pulsed laser deposition of plasmonic silver nanoparticle films in flowing gas at atmospheric pressure is described. The ablation was done using an excimer laser at 248 nm. Fast optical imaging shows that the ablation plume is captured by the flowing gas, and is expected to form a nanoparticle aerosol, which is carried 5 - 20 mm to the substrate. The dependence of the deposition rate on laser fluence, gas flow velocity, and target-substrate distance was investigated using electron microscopy and absorption spectroscopy of the deposited films...
September 8, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28882994/synthesis-of-mixed-hypermetallic-oxide-baoca-from-laser-cooled-reagents-in-an-atom-ion-hybrid-trap
#7
Prateek Puri, Michael Mills, Christian Schneider, Ionel Simbotin, John A Montgomery, Robin Côté, Arthur G Suits, Eric R Hudson
Hypermetallic alkaline earth (M) oxides of formula MOM have been studied under plasma conditions that preclude insight into their formation mechanism. We present here the application of emerging techniques in ultracold physics to the synthesis of a mixed hypermetallic oxide, BaOCa(+) These methods, augmented by high-level electronic structure calculations, permit detailed investigation of the bonding and structure, as well as the mechanism of its formation via the barrierless reaction of Ca ((3)PJ) with BaOCH3(+) Further investigations of the reaction kinetics as a function of collision energy over the range 0...
September 7, 2017: Science
https://www.readbyqxmd.com/read/28877242/comparative-quantitative-proteomic-analysis-of-disease-stratified-laser-captured-microdissected-human-islets-identifies-proteins-and-pathways-potentially-related-to-type-1-diabetes
#8
Julius O Nyalwidhe, Wojciech J Grzesik, Tanya C Burch, Michele L Semeraro, Tayab Waseem, Ivan C Gerling, Raghavendra G Mirmira, Margaret A Morris, Jerry L Nadler
Type 1 diabetes (T1D) is a chronic inflammatory disease that is characterized by autoimmune destruction of insulin-producing pancreatic beta cells. The goal of this study was to identify novel protein signatures that distinguish Islets from patients with T1D, patients who are autoantibody positive without symptoms of diabetes, and from individuals with no evidence of disease. High resolution high mass accuracy label free quantitative mass spectrometry analysis was applied to islets isolated by laser capture microdissection from disease stratified human pancreata from the Network for Pancreatic Organ Donors with Diabetes (nPOD), these included donors without diabetes, donors with T1D-associated autoantibodies in the absence of diabetes, and donors with T1D...
2017: PloS One
https://www.readbyqxmd.com/read/28875156/asporin-stably-expressed-in-the-surface-layer-of-mandibular-condylar-cartilage-and-augmented-in-the-deeper-layer-with-age
#9
Yutaka Miyamoto, Hiroyuki Kanzaki, Satoshi Wada, Sari Tsuruoka, Kanako Itohiya, Kenichi Kumagai, Yoshiki Hamada, Yoshiki Nakamura
Mandibular condylar cartilage (MCC) exhibits dual roles both articular cartilage and growth center. Of many growth factors, TGF-β has been implicated in the growth of articular cartilage including MCC. Recently, Asporin, decoy to TGF-β, was discovered and it blocks TGF-β signaling. Asporin is expressed in a variety of tissues including osteoarthritic articular cartilage, though there was no report of Asporin expression in MCC. In the present study, we investigated the temporal and spatial expression of Asporin in MCC...
December 2017: Bone Reports
https://www.readbyqxmd.com/read/28871549/laser-capture-microdissection-for-amplification-of-alternative-oxidase-aox-genes-in-target-tissues-in-daucus-carota-l
#10
Carla Ragonezi, Birgit Arnholdt-Schmitt
Laser microdissection provides a useful method for isolating specific cell types from complex biological samples for downstream applications. In contrast to the texture of mammalian cells, most plant tissues exhibit a cell organization with hard, cellulose-containing cell walls, large vacuoles, and air spaces, thus complicating tissue preparation and extraction of macromolecules such as DNA. In this study, we report a method that allows tissue-specific gene amplification. An improved perception of genetic identity of the entire plant can contribute to improved functional marker strategies...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28871082/stromal-and-epithelial-transcriptional-map-of-initiation-progression-and-metastatic-potential-of-human-prostate-cancer
#11
Svitlana Tyekucheva, Michaela Bowden, Clyde Bango, Francesca Giunchi, Ying Huang, Chensheng Zhou, Arrigo Bondi, Rosina Lis, Mieke Van Hemelrijck, Ove Andrén, Sven-Olof Andersson, R William Watson, Stephen Pennington, Stephen P Finn, Neil E Martin, Meir J Stampfer, Giovanni Parmigiani, Kathryn L Penney, Michelangelo Fiorentino, Lorelei A Mucci, Massimo Loda
While progression from normal prostatic epithelium to invasive cancer is driven by molecular alterations, tumor cells and cells in the cancer microenvironment are co-dependent and co-evolve. Few human studies to date have focused on stroma. Here, we performed gene expression profiling of laser capture microdissected normal non-neoplastic prostate epithelial tissue and compared it to non-transformed and neoplastic low-grade and high-grade prostate epithelial tissue from radical prostatectomies, each with its immediately surrounding stroma...
September 4, 2017: Nature Communications
https://www.readbyqxmd.com/read/28871006/post-transcriptional-regulation-of-mmp16-and-timp2-expression-via-mir-382-mir-410-and-mir-200b-in-endometrial-cancer
#12
Beata Rak, Dawid Mehlich, Filip Garbicz, Zofia Domosud, Wiktor Paskal, Janina M Marczewska, Paweł K Włodarski
BACKGROUND/AIM: The post-transcriptional regulation of matrix metalloproteinases (MMPs) via microRNAs (miRNAs) has been recently described in numerous human malignancies. However, the exact mechanisms of miRNA-mediated MMPs deregulation in endometrial cancer (EC) remain unclear. Herein, we aimed to analyze the expression of MMP2, MMP16 and TIMP2 and identify miRNAs that modulate their expression. MATERIALS AND METHODS: Protein expression was assessed by immunohistochemistry in formalin-fixed paraffin-embedded EC samples...
September 2017: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/28863668/gain-depletion-of-x-ray-framing-camera
#13
M Koga, H Shiraga
X-ray imaging is very useful to investigate imploded core plasma in inertial fusion experiments. We can obtain information from X-ray images, such as shape, density, and temperature. An X-ray framing camera (XFC) capable of taking two-dimensional, time-resolved X-ray images is used to capture the images. In previous work, we developed a numerical model of an XFC to analyze its X-ray image. The calculated results agreed qualitatively with experimental results. However, it was not accurate enough to determine the absolute value of the signal...
August 2017: Review of Scientific Instruments
https://www.readbyqxmd.com/read/28860973/particle-tracking-facilitates-real-time-capable-motion-correction-in-2d-or-3d-two-photon-imaging-of-neuronal-activity
#14
Samira Aghayee, Daniel E Winkowski, Zachary Bowen, Erin E Marshall, Matt J Harrington, Patrick O Kanold, Wolfgang Losert
The application of 2-photon laser scanning microscopy (TPLSM) techniques to measure the dynamics of cellular calcium signals in populations of neurons is an extremely powerful technique for characterizing neural activity within the central nervous system. The use of TPLSM on awake and behaving subjects promises new insights into how neural circuit elements cooperatively interact to form sensory perceptions and generate behavior. A major challenge in imaging such preparations is unavoidable animal and tissue movement, which leads to shifts in the imaging location (jitter)...
2017: Frontiers in Neural Circuits
https://www.readbyqxmd.com/read/28859241/mir-125b-1-and-mir-378a-are-predictive-biomarkers-for-the-efficacy-of-vaccine-treatment-against-colorectal-cancer
#15
Hironori Tanaka, Shoichi Hazama, Michihisa Iida, Ryouichi Tsunedomi, Hiroko Takenouchi, Masao Nakajima, Yukio Tokumitsu, Shinsuke Kanekiyo, Yoshitaro Shindo, Shinobu Tomochika, Yoshihiro Tokuhisa, Kazuhiko Sakamoto, Nobuaki Suzuki, Shigeru Takeda, Shigeru Yamamoto, Shigefumi Yoshino, Tomio Ueno, Yoshihiko Hamamoto, Yusuke Fujita, Hiroaki Tanaka, Ko Tahara, Ryoichi Shimizu, Kiyotaka Okuno, Koji Fujita, Masahiko Kuroda, Yusuke Nakamura, Hiroaki Nagano
Many clinical trials of peptide vaccines have been conducted. However, these vaccines have provided clinical benefits in only a small fraction of patients. The purpose of this study was to explore microRNAs (miRNAs) as novel predictive biomarkers for the efficacy of vaccine treatment against colorectal cancer. First, we performed microarray analysis of pretreatment cancer tissues in a phase I study, in which peptide vaccines alone were administered. Candidate miRNAs were selected by comparison of the better prognosis group with the poorer prognosis group...
August 31, 2017: Cancer Science
https://www.readbyqxmd.com/read/28854595/dormancy-and-activation-of-human-oocytes-from-primordial-and-primary-follicles-molecular-clues-to-oocyte-regulation
#16
E H Ernst, M L Grøndahl, S Grund, K Hardy, A Heuck, L Sunde, S Franks, C Y Andersen, P Villesen, K Lykke-Hartmann
STUDY QUESTION: Do specific transcriptome dynamics in human oocytes from primordial and primary follicles identify novel pathways in oocyte activation? SUMMARY ANSWER: The transcriptomic profiles in oocytes from primordial and primary follicles, respectively, revealed several new canonical pathways as putative mediators of oocyte dormancy and activation. WHAT IS KNOWN ALREADY: Cellular signaling pathways including PI3K/AKT and AKT/mTOR as well as TGF-β and IGF signaling are known to regulate the primordial-to-primary transition in mammalian follicle development...
August 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28852816/ir-maldesi-method-optimization-based-on-time-resolved-measurement-of-ion-yields
#17
Måns Ekelöf, David C Muddiman
In the field of mass spectrometry imaging, typical experiments involve ionization directly from complex samples with no pre-ionization separation, relying on high resolving power mass analyzers to separate ions of interest. When an ion trapping step is involved in the analysis, the dynamic range of the analysis may be limited by the capacity of the ion trap, which is easily exceeded. To minimize collection of undesired ambient species while maximizing collection of analyte signal, accurate timing between ion generation and collection is a requirement...
August 29, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28851950/biomechanical-properties-of-predator-induced-body-armour-in-the-freshwater-crustacean-daphnia
#18
Sebastian Kruppert, Martin Horstmann, Linda C Weiss, Ulrich Witzel, Clemens F Schaber, Stanislav N Gorb, Ralph Tollrian
The freshwater crustacean Daphnia is known for its ability to develop inducible morphological defences that thwart predators. These defences are developed only in the presence of predators and are realized as morphological shape alterations e.g. 'neckteeth' in D. pulex and 'crests' in D. longicephala. Both are discussed to hamper capture, handling or consumption by interfering with the predator's prey capture devices. Additionally, D. pulex and some other daphniids were found to armour-up and develop structural alterations resulting in increased carapace stiffness...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28844788/spatiotemporal-resolution-of-spinal-meningeal-and-parenchymal-inflammation-during-experimental-autoimmune-encephalomyelitis
#19
Bandana Shrestha, Xi Jiang, Shujun Ge, Debayon Paul, Peter Chianchiano, Joel S Pachter
Experimental autoimmune encephalomyelitis (EAE) induced by active immunization of C57BL/6 mice with peptide from myelin oligodendrocyte protein (MOG35-55), is a neuroinflammatory, demyelinating disease widely recognized as an animal model of multiple sclerosis (MS). Typically, EAE presents with an ascending course of paralysis, and inflammation that is predominantly localized to the spinal cord. Recent studies have further indicated that inflammation - in both MS and EAE - might initiate within the meninges and propagate from there to the underlying parenchyma...
August 24, 2017: Neurobiology of Disease
https://www.readbyqxmd.com/read/28843815/nucleoside-reverse-transcriptase-inhibitors-induced-hepatocellular-mitochondrial-dna-lesions-and-compensatory-enhancement-of-mitochondrial-function-and-dna-repair
#20
Qi Liang, Jing Zeng, Jian Wu, Luxin Qiao, Qinghai Chen, Dexi Chen, Yulin Zhang
As the backbone of combined antiretroviral therapy (cART), nucleoside reverse transcriptase inhibitors (NRTIs) are widely used in anti-HIV therapy. The long-term administration of NRTIs can result in mitochondrial dysfunction in certain HIV-1 infected patients. But NRTIs associated liver mitochondrial toxicity is not wellknown. Herein, the liver autopsy of AIDS patients and the liver tissues of the mice with 12 months of NRTI exposure were used to identify NRTIs associated liver toxicity with immunofluorescence, quantitative real-time PCR, Amplex red and horseradish peroxidase, and cloning and sequencing...
August 23, 2017: International Journal of Antimicrobial Agents
keyword
keyword
10072
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"