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https://www.readbyqxmd.com/read/28327472/spatiotemporal-treadmill-gait-measurements-using-a-laser-range-scanner-feasibility-study-of-the-healthy-young-adults
#1
S Tanabe, T Ii, S Koyama, E Saitoh, N Itoh, K Ohtsuka, Y Katoh, A Shimizu, Y Tomita
OBJECTIVE: Spatio-temporal parameters are typically used for gait analysis. Although these parameters are measured by sophisticated systems such as 3D motion capture system or optoelectronic bars, these systems cannot be deployed easily because of their high costs, large space requirements and elaborate set-up. The purpose of this study is to develope a system for measuring spatiotemporal gait parameters using a laser range scanner during treadmill gait. APPROACH: To calculate accurate spatiotemporal parameters, the differences between the laser range scanner measured values and the reference values obtained from a 3D motion capture system were investigated in thirty subjects...
March 22, 2017: Physiological Measurement
https://www.readbyqxmd.com/read/28325018/imquant-an-image-based-fluorescence-reader-for-quantitative-lateral-flow-immunoassays
#2
Kaushik Basak Chowdhury, Jayaraj Joseph, Jayaraman Kiruthi Vasan, Mohanasankar Sivaprakasam
Fluorescence immunoassays (FIA) have strong potential in enabling rapid quantitative immunological testing at point of care. Existing state of the art instruments employ laser scanning of FIA test kits, which require the use of electromechanical systems, making the instrument bulky, heavy and less reliable in the long term. There is a growing need for an extremely compact and reliable, motion-free, automated reader for accurate quantification of immunoassays. Here, we present ImQuant, an image based FIA reader with integrated monochromatic excitation source, optical filtering and real time image capture, assisted with an intuitive graphical user interface...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28322514/strategies-to-improve-cancer-photothermal-therapy-mediated-by-nanomaterials
#3
REVIEW
Duarte de Melo-Diogo, Cleide Pais-Silva, Diana R Dias, André F Moreira, Ilídio J Correia
The deployment of hyperthermia-based treatments for cancer therapy has captured the attention of different researchers worldwide. In particular, the application of light-responsive nanomaterials to mediate hyperthermia has revealed promising results in several pre-clinical assays. Unlike conventional therapies, these nanostructures can display a preferential tumor accumulation and thus mediate, upon irradiation with near-infrared light, a selective hyperthermic effect with temporal resolution. Different types of nanomaterials such as those based on gold, carbon, copper, molybdenum, tungsten, iron, palladium and conjugated polymers have been used for this photothermal modality...
March 21, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28316243/underwater-transparent-miniature-mechanical-hand-based-on-femtosecond-laser-induced-controllable-oil-adhesive-patterned-glass-for-oil-droplet-manipulation
#4
Jinglan Huo, Qing Yang, Feng Chen, Jiale Yong, Yao Fang, Jingzhou Zhang, Lin Liu, Xun Hou
The development of underwater superoleophobic surfaces makes researchers be swept up in boundless imagination of their applications, thereinto the oil manipulation based on such surface attracts much attention. Here, we show a simple and effective way to fabricate an underwater transparent miniature "mechanical hand" based on controllable oil-adhesive patterned glass prepared by femtosecond laser. The underwater oil-adhesive force of the patterned glasses that compose the "mechanical hand" device can be controlled from ultralow to ultrahigh through adjusting the different fraction of the untreated flat glass area to the laser-ablated rough area...
March 19, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28314739/intratumorous-heterogeneity-for-ras-mutations-in-a-treatment-na%C3%A3-ve-colorectal-tumour
#5
Sebastian Lunke, Belinda Lee, Sevastjan Kranz, Peter Gibbs, Paul Waring, Michael Christie
Activating mutations in KRAS and NRAS genes in patients with colorectal cancer (CRC) are associated with a lack of response to treatment with anti-epidermal growth factor receptor (EGFR) therapies. Mutations in these genes are thought to be mutually exclusive, however reports have described CRCs with two activating rat sarcoma (RAS) mutations. This has fuelled discussion about whether these mutations are the result of intratumorous heterogeneity, or if they are co-occurring in the same cancer cell clone. We present a case of a colorectal tumour with three RAS mutations detected during routine diagnostic testing...
March 17, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28302159/a-data-driven-approach-links-microglia-to-pathology-and-prognosis-in-amyotrophic-lateral-sclerosis
#6
Johnathan Cooper-Knock, Claire Green, Gabriel Altschuler, Wenbin Wei, Joanna J Bury, Paul R Heath, Matthew Wyles, Catherine Gelsthorpe, J Robin Highley, Alejandro Lorente-Pons, Tim Beck, Kathryn Doyle, Karel Otero, Bryan Traynor, Janine Kirby, Pamela J Shaw, Winston Hide
Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease that lacks a predictive and broadly applicable biomarker. Continued focus on mutation-specific upstream mechanisms has yet to predict disease progression in the clinic. Utilising cellular pathology common to the majority of ALS patients, we implemented an objective transcriptome-driven approach to develop noninvasive prognostic biomarkers for disease progression. Genes expressed in laser captured motor neurons in direct correlation (Spearman rank correlation, p < 0...
March 16, 2017: Acta Neuropathologica Communications
https://www.readbyqxmd.com/read/28297959/evolution-of-surface-structure-in-laser-preheated-perturbed-materials
#7
C A Di Stefano, E C Merritt, F W Doss, K A Flippo, A M Rasmus, D W Schmidt
We report an experimental and computational study investigating the effects of laser preheat on the hydrodynamic behavior of a material layer. In particular, we find that perturbation of the surface of the layer results in a complex interaction, in which the bulk of the layer develops density, pressure, and temperature structure and in which the surface experiences instability-like behavior, including mode coupling. A uniform one-temperature preheat model is used to reproduce the experimentally observed behavior, and we find that this model can be used to capture the evolution of the layer, while also providing evidence of complexities in the preheat behavior...
February 2017: Physical Review. E
https://www.readbyqxmd.com/read/28295065/measuring-anisotropy-ellipse-of-atmospheric-turbulence-by-intensity-correlations-of-laser-light
#8
Fei Wang, Italo Toselli, Jia Li, Olga Korotkova
An experimental study has been performed of a laser beam propagating horizontally through the near-ground atmosphere above a grassy field at the University of Miami (UM) Coral Gables campus. The average intensity, scintillation index, and intensity correlation function are measured in the receiver plane for three channels with different turbulent conditions and at three different heights above the ground. Our results reveal that along short links (210 m) only the intensity correlation function captures the anisotropic information of turbulence, corresponding to the refractive index anisotropy ellipse of atmospheric fluctuations...
March 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28294302/polyclonal-and-monoclonal-igg-binding-on-protein-a-resins-evidence-of-competitive-binding-effects
#9
Justin Weinberg, Shaojie Zhang, Gillian Crews, Edward Healy, Giorgio Carta, Todd Przybycien
Protein A (ProA) chromatography is used extensively in the biopharmaceutical industry for the selective capture of both polyclonal and monoclonal antibodies (mAbs). This work provides a comparison of the adsorptive behavior of a highly heterogeneous polyclonal hIgG versus that of a mAb as well as the behavior of their mixtures on representative ProA resins. Both pH gradient elution and frontal loading experiments using human polyclonal IgG (hIgG) reveal a distribution of IgG-ProA binding strengths likely associated with multiple IgG subclasses and the heterogeneity of the variable region...
March 14, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28280284/the-fiber-optic-system-for-the-advanced-topographic-laser-altimeter-system-atlas-instrument
#10
Melanie N Ott, Joe Thomes, Eleanya Onuma, Robert Switzer, Richard Chuska, Diana Blair, Erich Frese, Marc Matyseck
The Advanced Topographic Laser Altimeter System (ATLAS) Instrument has been in integration and testing over the past 18 months in preparation for the Ice, Cloud and Land Elevation Satellite - 2 (ICESat-2) Mission, scheduled to launch in 2017. ICESat-2 is the follow on to ICESat which launched in 2003 and operated until 2009. ATLAS will measure the elevation of ice sheets, glaciers and sea ice or the "cryosphere" (as well as terrain) to provide data for assessing the earth's global climate changes. Where ICESat's instrument, the Geo-Science Laser Altimeter (GLAS) used a single beam measured with a 70 m spot on the ground and a distance between spots of 170 m, ATLAS will measure a spot size of 10 m with a spacing of 70 cm using six beams to measure terrain height changes as small as 4 mm...
August 28, 2016: Proceedings of SPIE
https://www.readbyqxmd.com/read/28275314/ebm-regeneration-and-changes-in-ebm-component-mrna-expression-in-stromal-cells-after-corneal-injury
#11
Abirami Santhanam, Gustavo K Marino, Andre A M Torricelli, Steven E Wilson
PURPOSE: To investigate the production of the epithelial basement membrane (EBM) component mRNAs at time points before lamina lucida and lamina densa regeneration in anterior stromal cells after corneal injury that would heal with and without fibrosis. METHODS: Rabbit corneas were removed from 2 to 19 days after -4.5D or -9.0D photorefractive keratectomy (PRK) with the VISX S4 IR laser. Corneas were evaluated with transmission electron microscopy (TEM) for full regeneration of the lamina lucida and the lamina densa...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28272964/the-effectiveness-of-low-level-laser-therapy-on-pain-self-reported-hand-function-and-grip-strength-compared-to-placebo-or-sham-treatment-for-adults-with-carpal-tunnel-syndrome-a-systematic-review
#12
Marlette Burger, Réna Kriel, Andrea Damon, Amy Abel, Anisha Bansda, Marinique Wakens, Dawn Ernstzen
BACKGROUND: Carpal tunnel syndrome (CTS) is one of the most common peripheral neuropathies in the upper extremity. OBJECTIVE: The aim of this review was to systematically and critically appraise the available literature for the effectiveness of Low-Level Laser Therapy (LLLT) on pain, self-reported hand function, and grip strength compared to placebo treatment in adults with CTS. METHODOLOGY: Seven databases were searched from 2000 to March 2015 namely: Cinahl, Cochrane Library, EBSCOhost, PEDro, PubMed, Science Direct, and Scopus...
March 2017: Physiotherapy Theory and Practice
https://www.readbyqxmd.com/read/28269027/measurement-of-shank-angle-during-stance-using-laser-range-finders
#13
Pouya Amiri, Luke J MacLean, Robert E Kearney
Ankle joint stiffness, the dynamic relationship between the joint angle and the torque acting about it, plays an important role in the control of upright stance. In order to identify the contribution of ankle joint stiffness to stance control, ankle joint must be perturbed externally. One way to do this is to displace the foot that will cause shank movement. For identification, the ankle angle must be measured with high accuracy, for which we need to measure both foot and shank angles. However, most motion capture systems do not have the resolution and accuracy needed to measure the small ankle joint movements that occur during stance...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28264457/utilization-of-a-terrestrial-laser-scanner-for-the-calibration-of-mobile-mapping-systems
#14
Seunghwan Hong, Ilsuk Park, Jisang Lee, Kwangyong Lim, Yoonjo Choi, Hong-Gyoo Sohn
This paper proposes a practical calibration solution for estimating the boresight and lever-arm parameters of the sensors mounted on a Mobile Mapping System (MMS). On our MMS devised for conducting the calibration experiment, three network video cameras, one mobile laser scanner, and one Global Navigation Satellite System (GNSS)/Inertial Navigation System (INS) were mounted. The geometric relationships between three sensors were solved by the proposed calibration, considering the GNSS/INS as one unit sensor...
February 27, 2017: Sensors
https://www.readbyqxmd.com/read/28263850/laser-capture-micro-dissection-combined-with-next-generation-sequencing-analysis-of-cell-type-specific-deafness-gene-expression-in-the-mouse-cochlea
#15
Shin-Ya Nishio, Yutaka Takumi, Shin-Ichi Usami
Cochlear implantation (CI), which directly stimulates the cochlear nerves, is the most effective and widely used medical intervention for patients with severe to profound sensorineural hearing loss. The etiology of the hearing loss is speculated to have a major influence of CI outcomes, particularly in cases resulting from mutations in genes preferentially expressed in the spiral ganglion region. To elucidate precise gene expression levels in each part of the cochlea, we performed laser-capture micro dissection in combination with next-generation sequencing analysis and determined the expression levels of all known deafness-associated genes in the organ of Corti, spiral ganglion, lateral wall, and spiral limbs...
March 3, 2017: Hearing Research
https://www.readbyqxmd.com/read/28263413/species-specific-diversity-in-the-anatomical-and-physiological-organization-of-the-bnst-vta-pathway
#16
Jennifer Kaufling, Delphine Girard, Marlène Maitre, Thierry Leste-Lasserre, François Georges
The anteromedial part of the bed nucleus of the stria terminalis (amBNST) is a limbic structure innervating the ventral tegmental area (VTA) that is remarkably constant across species. The amBNST modulates fear and anxiety, and activation of VTA dopamine (DA) neurons by amBNST afferents seems to be the way by which stress controls motivational states associated with reward or aversion. Because fear learning and anxiety states can be expressed differently between rats and mice, we compared the functional connectivity between amBNST and the VTA-DA neurons in both species using consistant methodological approaches...
March 6, 2017: European Journal of Neuroscience
https://www.readbyqxmd.com/read/28263391/overexpression-of-lipid-metabolism-genes-and-pbx1-in-the-contralateral-breasts-of-women-with-estrogen-receptor-negative-breast-cancer
#17
Jun Wang, Ali Shidfar, David Ivancic, Manish Ranjan, Liannian Liu, Mi-Ran Choi, Vamsi Parimi, Demirkan B Gursel, Megan E Sullivan, Matthew S Najor, Abde M Abukhdeir, Denise Scholtens, Seema A Khan
Risk biomarkers for estrogen receptor (ER)-negative breast cancer have clear value for breast cancer prevention. We previously reported a set of lipid metabolism (LiMe) genes with high expression in the contralateral unaffected breasts (CUBs) of ER-negative cancer cases. We now further examine LiMe gene expression in both tumor and CUB, and investigate the role of Pre-B-cell leukemia homeobox-1 (PBX1) as a candidate common transcription factor for LiMe gene expression. mRNA was extracted from laser-capture microdissected epithelium from tumor and CUB of 84 subjects (28 ER-positive cases, 28 ER-negative cases, 28 healthy controls)...
March 6, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28261350/identifying-dcn-and-hspd1-as-potential-biomarkers-in-colon-cancer-using-2d-lc-ms-ms-combined-with-itraq-technology
#18
Guoqing Li, Maoyu Li, Xujun Liang, Zhefeng Xiao, Pengfei Zhang, Meiying Shao, Fang Peng, Yongheng Chen, Yuanyuan Li, Zhuchu Chen
Colon cancer is one of the most common types of gastrointestinal cancers and the fourth cause of cancer death worldwide. To discover novel diagnostic biomarkers for colon cancer and investigate potential mechanisms of oncogenesis, quantitative proteomic approach using iTRAQ-tagging and 2D-LC-MS/MS was performed to characterize proteins alterations in colon cancer and non-neoplastic colonic mucosa (NNCM) using laser capture microdissection-harvested from the two types of tissues, respectively. As a result, 188 DEPs were identified, and the differential expression of two DEPs (DCN and HSPD1) was further verified by Western blotting and immunohistochemistry...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28257480/site-specific-gene-expression-profiling-as-a-novel-strategy-for-unravelling-keloid-disease-pathobiology
#19
N Jumper, T Hodgkinson, R Paus, A Bayat
Keloid disease (KD) is a fibroproliferative cutaneous tumour characterised by heterogeneity, excess collagen deposition and aggressive local invasion. Lack of a validated animal model and resistance to a multitude of current therapies has resulted in unsatisfactory clinical outcomes of KD management. In order to address KD from a new perspective, we applied for the first time a site-specific in situ microdissection and gene expression profiling approach, through combined laser capture microdissection and transcriptomic array...
2017: PloS One
https://www.readbyqxmd.com/read/28256794/laser-capture-microdissection-enables-transcriptomic-analysis-of-dividing-and-quiescent-liver-stages-of-plasmodium-relapsing-species
#20
Roger Cubi, Shruthi S Vembar, Anne Biton, Jean-Francois Franetich, Mallaury Bordessoulles, Daniel Sossau, Gigliola Zhanghi, Henriette Bosson-Vanga, Magalie Benard, Alicia Moreno, Nathalie Dereuddre-Bosquet, Roger Le Grand, Artur Scherf, Dominique Mazier
Dormant liver stage forms (hypnozoites) of the malaria parasite Plasmodium vivax present major hurdles to control and eradicate infection. Despite major research efforts, the molecular composition of hypnozoites remains ill defined. Here, we applied a combination of state-of-the-art technologies to generate the first transcriptome of hypnozoites. We developed a robust laser dissection microscopy protocol to isolate individual P. cynomolgi hypnozoites and schizonts from infected monkey hepatocytes and optimized RNA-seq analysis to obtain the first transcriptomes of these stages...
March 3, 2017: Cellular Microbiology
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