keyword
MENU ▼
Read by QxMD icon Read
search

Laser capture

keyword
https://www.readbyqxmd.com/read/29159276/linked-color-imaging-lci-a-novel-image-enhanced-endoscopy-technology-emphasizes-the-color-of-early-gastric-cancer
#1
Hiromitsu Kanzaki, Ryuta Takenaka, Yoshiro Kawahara, Daisuke Kawai, Yuka Obayashi, Yuki Baba, Hiroyuki Sakae, Tatsuhiro Gotoda, Yoshiyasu Kono, Ko Miura, Masaya Iwamuro, Seiji Kawano, Takehiro Tanaka, Hiroyuki Okada
Background and study aims:  Linked color imaging (LCI) and blue laser imaging (BLI) are novel image-enhanced endoscopy technologies with strong, unique color enhancement. We investigated the efficacy of LCI and BLI-bright compared to conventional white light imaging (WLI) by measuring the color difference between early gastric cancer lesions and the surrounding mucosa. Patients and methods:  Images of early gastric cancer scheduled for endoscopic submucosal dissection were captured by LCI, BLI-bright, and WLI under the same conditions...
October 2017: Endoscopy International Open
https://www.readbyqxmd.com/read/29153978/the-endometria-of-women-with-endometriosis-exhibit-dysfunctional-expression-of-complement-regulatory-proteins-during-the-mid-secretory-phase
#2
Wilder A Palomino, Chandrakant Tayade, Felipe Argandoña, Luigi Devoto, Steven L Young, Bruce A Lessey
The control of complement activation within embryo-endometrium environment is critical for embryo survival. Cell evasion from complement attack requires interaction of complement regulatory proteins (CRPs) with cell adhesion αvβ3 integrin. We aim to compare the expression of CRPs in endometria of women with and without endometriosis and to examine the molecular interaction of decay accelerating factor (DAF) with αvβ3 integrin. Endometrial expression of Membrane cofactor protein (CD46), Decay accelerating factor (DAF), Membrane attack complex inhibitory factor (CD59) and β3 integrin subunit were determined through menstrual cycle by immunohistochemistry...
October 27, 2017: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/29147002/capturing-an-initial-intermediate-during-the-p450nor-enzymatic-reaction-using-time-resolved-xfel-crystallography-and-caged-substrate
#3
Takehiko Tosha, Takashi Nomura, Takuma Nishida, Naoya Saeki, Kouta Okubayashi, Raika Yamagiwa, Michihiro Sugahara, Takanori Nakane, Keitaro Yamashita, Kunio Hirata, Go Ueno, Tetsunari Kimura, Tamao Hisano, Kazumasa Muramoto, Hitomi Sawai, Hanae Takeda, Eiichi Mizohata, Ayumi Yamashita, Yusuke Kanematsu, Yu Takano, Eriko Nango, Rie Tanaka, Osamu Nureki, Osami Shoji, Yuka Ikemoto, Hironori Murakami, Shigeki Owada, Kensuke Tono, Makina Yabashi, Masaki Yamamoto, Hideo Ago, So Iwata, Hiroshi Sugimoto, Yoshitsugu Shiro, Minoru Kubo
Time-resolved serial femtosecond crystallography using an X-ray free electron laser (XFEL) in conjunction with a photosensitive caged-compound offers a crystallographic method to track enzymatic reactions. Here we demonstrate the application of this method using fungal NO reductase, a heme-containing enzyme, at room temperature. Twenty milliseconds after caged-NO photolysis, we identify a NO-bound form of the enzyme, which is an initial intermediate with a slightly bent Fe-N-O coordination geometry at a resolution of 2...
November 17, 2017: Nature Communications
https://www.readbyqxmd.com/read/29140985/notch-signaling-and-progenitor-ductular-reaction-in-steatohepatitis
#4
Carola M Morell, Romina Fiorotto, Marica Meroni, Aileen Raizner, Barbara Torsello, Massimiliano Cadamuro, Gaia Spagnuolo, Eleanna Kaffe, Salvatore Sutti, Emanuele Albano, Mario Strazzabosco
BACKGROUND AND OBJECTIVE: Persistent hepatic progenitor cells (HPC) activation resulting in ductular reaction (DR) is responsible for pathologic liver repair in cholangiopathies. Also, HPC/DR expansion correlates with fibrosis in several chronic liver diseases, including steatohepatitis. Increasing evidence indicates Notch signaling as a key regulator of HPC/DR response in biliary and more in general liver injuries. Therefore, we aimed to investigate the role of Notch during HPC/DR activation in a mouse model of steatohepatitis...
2017: PloS One
https://www.readbyqxmd.com/read/29137483/molecular-profiling-in-iga-nephropathy-and-focal-and-segmental-glomerulosclerosis
#5
I Tycová, P Hrubá, D Maixnerová, E Girmanová, P Mrázová, L Straňavová, R Zachoval, M Merta, J Slatinská, M Kollár, E Honsová, V Tesař, O Viklický
The aim of the study was to characterize by molecular profiling two glomerular diseases: IgA nephropathy (IgAN) and focal segmental glomerulosclerosis (FSGS) and to identify potential molecular markers of IgAN and FSGS progression. The expressions of 90 immune-related genes were compared in biopsies of patients with IgAN (n=33), FSGS (n=17) and in controls (n=11) using RT-qPCR. To identify markers of disease progression, gene expression was compared between progressors and non-progressors in 1 year follow-up...
November 10, 2017: Physiological Research
https://www.readbyqxmd.com/read/29137350/il2rg-identified-as-overexpressed-by-rna-seq-profiling-of-pancreatic-intraepithelial-neoplasia-mediates-pancreatic-cancer-growth
#6
Michael Ayars, Eileen O'Sullivan, Anne Macgregor-Das, Koji Shindo, Haeryoung Kim, Michael Borges, Jun Yu, Ralph H Hruban, Michael Goggins
Pancreatic ductal adenocarcinoma evolves from precursor lesions, the most common of which is pancreatic intraepithelial neoplasia (PanIN). We performed RNA-sequencing analysis of laser capture microdissected PanINs and normal pancreatic duct cells to identify differentially expressed genes between PanINs and normal pancreatic duct, and between low-grade and high-grade PanINs. One of the most highly overexpressed transcripts identified in PanIN is interleukin-2 receptor subunit gamma (IL2RG) encoding the common gamma chain, IL2Rγ...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29130948/mapping-genomic-scaffolds-to-chromosomes-using-laser-capture-microdissection-in-application-to-hawaiian-picture-winged-drosophila
#7
Lin Kang, Phillip George, Donald K Price, Igor Sharakhov, Pawel Michalak
Next-generation sequencing technologies have led to a decreased cost and an increased throughput in genome sequencing. Yet, many genome assemblies based on short sequencing reads have been assembled only to the scaffold level due to the lack of sufficient chromosome mapping information. Traditional ways of mapping scaffolds to chromosomes require a large amount of laboratory work and time to generate genetic and/or physical maps. To address this problem, we conducted a rapid technique which uses laser capture microdissection and enables mapping scaffolds of de novo genome assemblies directly to chromosomes in Hawaiian picture-winged Drosophila...
November 8, 2017: Cytogenetic and Genome Research
https://www.readbyqxmd.com/read/29130192/lcm-seq-a-method-for-spatial-transcriptomic-profiling-using-laser-capture-microdissection-coupled-with-polya-based-rna-sequencing
#8
Susanne Nichterwitz, Julio Aguila Benitez, Rein Hoogstraaten, Qiaolin Deng, Eva Hedlund
LCM-seq couples laser capture microdissection of cells from frozen tissues with polyA-based RNA sequencing and is applicable to single neurons. The method utilizes off-the-shelf reagents and direct lysis of the cells without RNA purification, making it a simple and relatively cheap method with high reproducibility and sensitivity compared to previous methods. The advantage with LCM-seq is also that tissue sections are kept intact and thus the positional information of each cell is preserved.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29123674/characterization-of-temporal-coherence-of-hard-x-ray-free-electron-laser-pulses-with-single-shot-interferograms
#9
Taito Osaka, Takashi Hirano, Yuki Morioka, Yasuhisa Sano, Yuichi Inubushi, Tadashi Togashi, Ichiro Inoue, Kensuke Tono, Aymeric Robert, Kazuto Yamauchi, Jerome B Hastings, Makina Yabashi
Temporal coherence is one of the most fundamental characteristics of light, connecting to spectral information through the Fourier transform relationship between time and frequency. Interferometers with a variable path-length difference (PLD) between the two branches have widely been employed to characterize temporal coherence properties for broad spectral regimes. Hard X-ray interferometers reported previously, however, have strict limitations in their operational photon energies, due to the specific optical layouts utilized to satisfy the stringent requirement for extreme stability of the PLD at sub-ångström scales...
November 1, 2017: IUCrJ
https://www.readbyqxmd.com/read/29118098/x-ray-free-electron-laser-opportunities-for-drug-discovery
#10
REVIEW
Robert K Y Cheng, Rafael Abela, Michael Hennig
Past decades have shown the impact of structural information derived from complexes of drug candidates with their protein targets to facilitate the discovery of safe and effective medicines. Despite recent developments in single particle cryo-electron microscopy, X-ray crystallography has been the main method to derive structural information. The unique properties of X-ray free electron laser (XFEL) with unmet peak brilliance and beam focus allow X-ray diffraction data recording and successful structure determination from smaller and weaker diffracting crystals shortening timelines in crystal optimization...
November 8, 2017: Essays in Biochemistry
https://www.readbyqxmd.com/read/29116514/combine-phage-antibody-display-library-selection-on-patient-tissue-specimens-with-laser-capture-microdissection-to-identify-novel-human-antibodies-targeting-clinically-relevant-tumor-antigens
#11
Yang Su, Scott Bidlingmaier, Nam-Kyung Lee, Bin Liu
A functional approach to generate tumor-targeting human monoclonal antibodies is through selection of phage antibody display libraries directly on tumor cells. Although technically convenient, the use of cancer cell lines for the selection has limitations as those cell lines often undergo genetic and epigenetic changes during prolonged in vitro culture and alter their cell surface antigen expression profile. The key is to develop a technology that allows selection of phage antibody display libraries on tumor cells in situ residing in their natural tissue microenvironment...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29103202/stain-free-histopathology-of-basal-cell-carcinoma-by-dual-vibration-resonance-frequency-cars-microscopy
#12
Norbert Kiss, Ádám Krolopp, Kende Lőrincz, András Bánvölgyi, Róbert Szipőcs, Norbert Wikonkál
Basal cell carcinoma (BCC) is the most common malignancy in Caucasians. Nonlinear microscopy has been previously utilized for the imaging of BCC, but the captured images do not correlate with H&E staining. Recently, Freudiger et al. introduced a novel method to visualize tissue morphology analogous to H&E staining, using coherent anti-Stokes Raman scattering (CARS) technique. In our present work, we introduce a novel algorithm to post-process images obtained from dual vibration resonance frequency (DVRF) CARS measurements to acquire high-quality pseudo H&E images of BCC samples...
November 4, 2017: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/29101151/laser-scanning-in-vivo-confocal-microscopy-ivcm-for-evaluating-human-corneal-sub-basal-nerve-plexus-parameters-protocol-for-a-systematic-review
#13
Manikkuwadura Eranda Harshan De Silva, Alexis Ceecee Zhang, Amalia Karahalios, Holly Rose Chinnery, Laura Elizabeth Downie
INTRODUCTION: Laser scanning in vivo confocal microscopy (IVCM) enables non-invasive, high-resolution imaging of the cornea. In recent years, there has been a vast increase in researchers using laser scanning IVCM to image and quantify corneal nerve parameters. However, a range of methodological approaches have been adopted. The primary aim of this systematic review is to critically appraise the reported method(s) of primary research studies that have used laser scanning IVCM to quantify corneal sub-basal nerve plexus (SBNP) parameters in humans, and to examine corneal nerve parameters in healthy individuals...
November 3, 2017: BMJ Open
https://www.readbyqxmd.com/read/29097624/dnaj-homolog-subfamily-b-member-9-is-a-putative-autoantigen-in-fibrillary-gn
#14
Nicole K Andeen, Han-Yin Yang, Dao-Fu Dai, Michael J MacCoss, Kelly D Smith
Fibrillary GN is a rare form of GN of uncertain pathogenesis that is characterized by the glomerular accumulation of randomly arranged, nonbranching fibrils (12-24 nm) composed of Ig and complement proteins. In this study, we used mass spectrometry to comprehensively define the glomerular proteome in fibrillary GN compared with that in controls and nonfibrillary GN renal diseases. We isolated glomeruli from formalin-fixed and paraffin-embedded biopsy specimens using laser capture microdissection and analyzed them with liquid chromatography and data-dependent tandem mass spectrometry...
November 2, 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/29095436/rna-sequencing-based-comparative-analysis-of-human-hepatic-progenitor-cells-and-their-niche-from-alcoholic-steatohepatitis-livers
#15
An Ceulemans, Stefaan Verhulst, Matthias Van Haele, Olivier Govaere, Juan-Jose Ventura, Leo A van Grunsven, Tania Roskams
Hepatic progenitor cells (HPCs) are small cells with a relative large oval nucleus and a scanty cytoplasm situated in the canals of Hering that express markers of (immature) hepatocytes and cholangiocytes. HPCs are present in large numbers in alcoholic steatohepatitis (ASH), one of the leading causes of chronic liver disease. To date, the mechanisms responsible for proliferation and differentiation of human HPCs are still poorly understood and the role of HPCs in ASH development is unknown. In this study, we aimed to characterise human HPCs and their interactions with other cells through comparison, on both protein and RNA level, of HPC-enriched cell populations from adult human liver tissue using different isolation methods...
November 2, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/29091674/fleet-velocimetry-for-combustion-and-flow-diagnostics
#16
Nicholas J DeLuca, Richard B Miles, Naibo Jiang, Waruna D Kulatilaka, Anil K Patnaik, James R Gord
We report the use of femtosecond laser electronic excitation tagging (FLEET) for velocimetry at a 100-kHz imaging rate. Sequential, single-shot, quantitative velocity profiles of an underexpanded supersonic nitrogen jet were captured at a 100-kHz rate. The signal and lifetime characteristics of the FLEET emission were investigated in a methane flame above a Hencken burner at varying equivalence ratios, and room temperature gas mixtures involving air, methane, and nitrogen. In the post-flame region of the Hencken burner, the emission lifetime was measured as two orders of magnitude lower than lab air conditions...
November 1, 2017: Applied Optics
https://www.readbyqxmd.com/read/29088992/circadian-profiling-of-amino-acids-in-the-scn-and-cerebral-cortex-by-laser-capture-microdissection-mass-spectrometry
#17
Jean-Michel Fustin, Sachise Karakawa, Hitoshi Okamura
The suprachiasmatic nucleus (SCN) is an extremely robust self-sustained oscillator, containing virtually the same molecular clock present in other tissues in the body but, in addition, endowed with tight intercellular coupling dependent on multiple neurotransmitter systems that allow the SCN to function as the "master clock." Several studies on the circadian SCN transcriptome have been published and compared with the transcriptome of other tissues, but the recent focus shift toward the circadian metabolome and the importance of small molecules for circadian timekeeping has so far been limited to macroscopic tissues such as the liver...
October 1, 2017: Journal of Biological Rhythms
https://www.readbyqxmd.com/read/29083854/applications-of-photogating-and-time-resolved-spectroscopy-to-mechanistic-studies-of-hydrogenases
#18
Brandon L Greene, Gregory E Vansuch, Bryant C Chica, Michael W W Adams, R Brian Dyer
Rapid and facile redox chemistry is exemplified in nature by the oxidoreductases, the class of enzymes that catalyze electron transfer (ET) from a donor to an acceptor. The key role of oxidoreductases in metabolism and biosynthesis has imposed evolutionary pressure to enhance enzyme efficiency, pushing some toward the diffusion limit. Understanding the detailed molecular mechanisms of these highly optimized enzymes would provide an important foundation for the rational design of catalysts for multielectron chemistry, including fuel production...
October 30, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/29082409/low-temperature-kinetics-and-theoretical-studies-of-the-reaction-cn-ch3nh2-a-potential-source-of-cyanamide-and-methyl-cyanamide-in-the-interstellar-medium
#19
Chantal Sleiman, Gisèle El Dib, Marzio Rosi, Dimitrios Skouteris, Nadia Balucani, André Canosa
The reaction between cyano radicals (which are ubiquitous in interstellar clouds) and methylamine (a molecule detected in various interstellar sources) has been investigated in a synergistic experimental and theoretical study. The reaction has been found to be very fast in the entire range of temperatures investigated (23-297 K) by using a CRESU apparatus coupled to pulsed laser photolysis - laser induced fluorescence. The global experimental rate coefficient is given by In addition, dedicated electronic structure calculations of the underlying potential energy surface have been performed, together with capture theory and RRKM calculations...
October 30, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29081750/coherence-and-coupling-functions-reveal-microvascular-impairment-in-treated-hypertension
#20
Valentina Ticcinelli, Tomislav Stankovski, Dmytro Iatsenko, Alan Bernjak, Adam E Bradbury, Andrew R Gallagher, Peter B M Clarkson, Peter V E McClintock, Aneta Stefanovska
The complex interactions that give rise to heart rate variability (HRV) involve coupled physiological oscillators operating over a wide range of different frequencies and length-scales. Based on the premise that interactions are key to the functioning of complex systems, the time-dependent deterministic coupling parameters underlying cardiac, respiratory and vascular regulation have been investigated at both the central and microvascular levels. Hypertension was considered as an example of a globally altered state of the complex dynamics of the cardiovascular system...
2017: Frontiers in Physiology
keyword
keyword
10072
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"