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https://www.readbyqxmd.com/read/28206760/nano-engineered-eggshell-silver-tailored-co-polyester-polymer-blend-film-with-antimicrobial-properties
#1
Boniface J Tiimob, Gregory Mwinyelle, Woubit Abdela, Temesgen Samuel, Shaik Jeelani, Vijaya K Rangari
In this study, the reinforcement effect of different proportions of eggshell/silver nanomaterial (ES-Ag) on structural and antimicrobial properties of 70/30 poly (butylene-co-adipate terephthalate)/polylactic acid (PBAT/PLA) immiscible blends were investigated. The ES-Ag was synthesized using a single step ball milling process, and characterized with X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM). These results confirmed the existence of silver nanoparticles (Ag NPs) in the interstitial spaces of the eggshell particles...
February 16, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28056787/software-updates-in-the-illumina-hiseq-platform-affect-whole-genome-bisulfite-sequencing
#2
Hidehiro Toh, Kenjiro Shirane, Fumihito Miura, Naoki Kubo, Kenji Ichiyanagi, Katsuhiko Hayashi, Mitinori Saitou, Mikita Suyama, Takashi Ito, Hiroyuki Sasaki
BACKGROUND: Methylation of cytosine in genomic DNA is a well-characterized epigenetic modification involved in many cellular processes and diseases. Whole-genome bisulfite sequencing (WGBS), such as MethylC-seq and post-bisulfite adaptor tagging sequencing (PBAT-seq), uses the power of high-throughput DNA sequencers and provides genome-wide DNA methylation profiles at single-base resolution. However, the accuracy and consistency of WGBS outputs in relation to the operating conditions of high-throughput sequencers have not been explored...
January 5, 2017: BMC Genomics
https://www.readbyqxmd.com/read/27973831/partial-and-complete-wetting-in-ultralow-interfacial-tension-multiphase-blends-with-polylactide
#3
Ali M Zolali, Basil D Favis
The control of phase structuring in multiphase blends of polylactide (PLA) with other polymers is a viable approach to promote its broader implementation. In this article, ternary and quaternary blends of PLA with poly(butylene succinate) (PBS), poly(butylene adipate-co-terephthalate) (PBAT), and poly(3-hydroxybutyrate-co-hydroxyvalerate) (PHBV) are prepared by melt blending. The interfacial tensions between components are measured using three different techniques, and a Fourier transform infrared imaging technique is developed for the purpose of unambiguous phase identification...
December 15, 2016: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/27940546/discovery-of-polyesterases-from-moss-associated-microorganisms
#4
Christina Andrea Müller, Veronika Perz, Christoph Provasnek, Felice Quartinello, Georg M Guebitz, Gabriele Berg
: The growing pollution of the environment with plastic debris is a global threat which urgently requires biotechnological solutions. Enzymatic recycling not only prevents pollution but also would allow recovery of valuable building blocks. Therefore, we explored the existence of microbial polyesterases in microbial communities associated with the Sphagnum magellanicum moss, a key species within unexploited bog ecosystems. This resulted in the identification of six novel esterases, which were isolated, cloned, and heterologously expressed in Escherichia coli The esterases were found to hydrolyze the copolyester poly(butylene adipate-co-butylene terephthalate) (PBAT) and the oligomeric model substrate bis[4-(benzoyloxy)butyl] terephthalate (BaBTaBBa)...
February 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27872998/ppest-is-a-novel-pbat-degrading-polyesterase-identified-by-proteomic-screening-of-pseudomonas-pseudoalcaligenes
#5
Paal W Wallace, Karolina Haernvall, Doris Ribitsch, Sabine Zitzenbacher, Matthias Schittmayer, Georg Steinkellner, Karl Gruber, Georg M Guebitz, Ruth Birner-Gruenberger
A novel esterase, PpEst, that hydrolyses the co-aromatic-aliphatic polyester poly(1,4-butylene adipate-co-terephthalate) (PBAT) was identified by proteomic screening of the Pseudomonas pseudoalcaligenes secretome. PpEst was induced by the presence of PBAT in the growth media and had predicted arylesterase (EC 3.1.1.2) activity. PpEst showed polyesterase activity on both whole and milled PBAT film releasing terephthalic acid and 4-(4-hydroxybutoxycarbonyl)benzoic acid while end product inhibition by 4-(4-hydroxybutoxycarbonyl)benzoic acid was observed...
November 21, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27770894/sepiolite-as-a-promising-nanoclay-for-nano-biocomposites-based-on-starch-and-biodegradable-polyester
#6
J B Olivato, J Marini, F Yamashita, E Pollet, M V E Grossmann, L Avérous
The effects of sepiolite addition (0, 1, 3 and 5wt%) were evaluated on dynamic-mechanical behaviour, water uptake, thermal and optical properties of thermoplastic starch (TPS)/poly (butylene adipate-co-terephthalate) (PBAT) nano-biocomposites, with different TPS/PBAT (w/w) ratios and nanofiller contents. The results highlighted the improvement of the dynamic-mechanical behaviour with the addition of sepiolite, producing high performance materials. An increase of 25°C in the Tg of TPS was recorded by DMTA analysis at sepiolite content of 5wt%...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27759212/replication-of-genome-wide-association-study-gwas-susceptibility-loci-in-a-latino-bipolar-disorder-cohort
#7
Suzanne Gonzalez, Jayanta Gupta, Erika Villa, Indika Mallawaarachchi, Marco Rodriguez, Mercedes Ramirez, Juan Zavala, Regina Armas, Albana Dassori, Javier Contreras, Deborah Flores, Alvaro Jerez, Alfonso Ontiveros, Humberto Nicolini, Michael Escamilla
OBJECTIVES: Recent genome-wide association studies (GWASs) have identified numerous putative genetic polymorphisms associated with bipolar disorder (BD) and/or schizophrenia (SC). We hypothesized that a portion of these polymorphisms would also be associated with BD in the Latino American population. To identify such regions, we tested previously identified genetic variants associated with BD and/or SC and ancestral haploblocks containing these single nucleotide polymorphisms (SNPs) in a sample of Latino subjects with BD...
September 2016: Bipolar Disorders
https://www.readbyqxmd.com/read/27724793/poly-butylene-adipate-co-terephthalate-scaffolds-processing-structural-characteristics-and-cellular-responses
#8
Aysu Arslan, Soner Çakmak, Alper Cengiz, Menemşe Gümüşderelioğlu
The aliphatic-aromatic copolyester, poly(butylene adipate-co-terephthalate) (PBAT) combines good mechanical and thermal properties with biodegradation ability. However, until now, researches in its potential medical use remain limited. Only in a few studies blends of PBAT with routinely used biocompatible polymers had been prepared and investigated regarding tissue engineering applications. Therefore, in this study, we decided to determine processability of neat PBAT as a scaffold material for bone tissue by using different fabrication methods i...
October 10, 2016: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/27616049/structural-reorganization-of-cnc-in-injection-molded-cnc-pbat-materials-under-thermal-annealing
#9
Marcos Mariano, Nadia El Kissi, Alain Dufresne
Composite materials were prepared by extrusion and injection molding from polybutyrate adipate terephthalate (PBAT) and high aspect ratio cellulose nanocrystals (CNCs) extracted from capim dourado fibers. Three CNC contents were used, corresponding to 0.5, 1, and 2 times the theoretical percolation threshold. Small-amplitude oscillary shear (SAOS) experiments show that as the CNC content increases, a more elastic behavior is observed but no percolating network can form within the polymeric matrix as a result of the high shear rates involved during the injection-molding process...
October 4, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/27460100/comparison-of-sequential-therapy-and-amoxicillin-tetracycline-containing-bismuth-quadruple-therapy-for-the-first-line-eradication-of-helicobacter-pylori-a-prospective-multi-center-randomized-clinical-trial
#10
RANDOMIZED CONTROLLED TRIAL
Ju Yup Lee, Nayoung Kim, Kyung Sik Park, Hyun Jin Kim, Seon Mee Park, Gwang Ho Baik, Ki-Nam Shim, Jung Hwan Oh, Suck Chei Choi, Sung Eun Kim, Won Hee Kim, Seon-Young Park, Gwang Ha Kim, Bong Eun Lee, Yunju Jo, Su Jin Hong
BACKGROUND: The <80 % Helicobacter pylori eradication rate with sequential therapy is unsatisfactory. Modified bismuth quadruple therapy, replacing metronidazole with amoxicillin, could be promising because H. pylori resistance to tetracycline or to amoxicillin is relatively low. A 14-day modified bismuth quadruple protocol as first-line H. pylori treatment was compared with 10-day sequential therapy. METHODS: In total, 390 H. pylori-infected subjects participated in the randomized clinical trial: 10-day sequential therapy (40 mg pantoprazole plus 1 g amoxicillin twice a day for 5 days, then 40 mg pantoprazole and 500 mg clarithromycin twice a day and 500 mg metronidazole three times a day for 5 days) or 14-day modified bismuth quadruple therapy (40 mg pantoprazole, 600 mg bismuth subcitrate, 1 g tetracycline, and 1 g amoxicillin, twice a day)...
July 26, 2016: BMC Gastroenterology
https://www.readbyqxmd.com/read/27343611/facile-synthesis-of-thiol-and-alkynyl-contained-sers-reporter-molecular-and-its-usage-in-assembly-of-polydopamine-protected-bioorthogonal-sers-tag-for-live-cell-imaging
#11
Ling Zhang, Ren Zhang, Mingxia Gao, Xiangmin Zhang
A bioorthogonal Raman reporter-embedded Au-core and polydopamine-shell nanoprobe was initially designed and synthesized for live cell surface-enhanced Raman scattering (SERS) imaging in Raman-silent region. The firstly synthetic bioorthogonal Raman reporter (E)-2- ((4-(phenylethynyl) benzylidene)amino)ethanethiol (PBAT) provided intense Raman signal at 2220cm(-1) in Raman-silent region. And its synthetic method was environmentally benign, high yield and easy in operation. In addition, the hydrophobicity of PBAT led to slightly aggregation of gold nanoparticles, which enhance the SERS intensity of the nanoprobe through hot spots...
September 1, 2016: Talanta
https://www.readbyqxmd.com/read/27314249/enhancing-the-mechanical-properties-of-biodegradable-polymer-blends-using-tubular-nanoparticle-stitching-of-the-interfaces
#12
Yichen Guo, Shan He, Kai Yang, Yuan Xue, Xianghao Zuo, Yingjie Yu, Ying Liu, Chung-Chueh Chang, Miriam H Rafailovich
"Green" polymer nanocomposites were made by melt blending biodegradable poly(lactic acid) (PLA) and poly(butylene adipate-co-butylene terephthalate) (PBAT) with either montmorillonite clays (Cloisite Na(+)), halloysite nanotubes (HNTs), the resorcinol diphenyl phosphate (RDP)-coated Cloisite Na(+), and coated HNTs. A technique for measuring the work of adhesion (Wa) between nanoparticles and their matrixes was used to determine the dispersion preference of the nanoparticles in the PLA/PBAT blend system. Transmission electron microscopy (TEM) images of thin sections indicated that even though both RDP-coated nanotubes and clay platelets segregated to the interfacial regions between the two immiscible polymers, only the platelets, having the larger specific surface area, were able to reduce the PBAT domain sizes...
July 13, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/26981550/data-on-synthesis-of-oligomeric-and-polymeric-poly-butylene-adipate-co-butylene-terephthalate-model-substrates-for-the-investigation-of-enzymatic-hydrolysis
#13
Veronika Perz, Klaus Bleymaier, Carsten Sinkel, Ulf Kueper, Melanie Bonnekessel, Doris Ribitsch, Georg M Guebitz
The aliphatic-aromatic copolyester poly(butylene adipate-co-butylene terephthalate) (PBAT), also known as ecoflex, contains adipic acid, 1,4-butanediol and terephthalic acid and is proven to be compostable [1], [2], [3]). We describe here data for the synthesis and analysis of poly(butylene adipate-co-butylene terephthalate variants with different adipic acid:terephatalic acid ratios and 6 oligomeric PBAT model substrates. Data for the synthesis of the following oligomeric model substrates are described: mono(4-hydroxybutyl) terephthalate (BTa), bis(4-(hexanoyloxy)butyl) terephthalate (HaBTaBHa), bis(4-(decanoyloxy)butyl) terephthalate (DaBTaBDa), bis(4-(tetradecanoyloxy)butyl) terephthalate (TdaBTaBTda), bis(4-hydroxyhexyl) terephthalate (HTaH) and bis(4-(benzoyloxy)butyl) terephthalate (BaBTaBBa)...
June 2016: Data in Brief
https://www.readbyqxmd.com/read/26959220/super-robust-polylactide-barrier-films-by-building-densely-oriented-lamellae-incorporated-with-ductile-in-situ-nanofibrils-of-poly-butylene-adipate-co-terephthalate
#14
Sheng-Yang Zhou, Hua-Dong Huang, Xu Ji, Ding-Xiang Yan, Gan-Ji Zhong, Benjamin S Hsiao, Zhong-Ming Li
Remarkable combination of excellent gas barrier performance, high strength, and toughness was realized in polylactide (PLA) composite films by constructing the supernetworks of oriented and pyknotic crystals with the assistance of ductile in situ nanofibrils of poly(butylene adipate-co-terephthalate) (PBAT). On the basis that the permeation of gas molecules through polymer materials with anisotropic structure would be more frustrated, we believe that oriented crystalline textures cooperating with inerratic amorphism can be favorable for the enhancement of gas barrier property...
March 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/26878094/an-esterase-from-anaerobic-clostridium-hathewayi-can-hydrolyze-aliphatic-aromatic-polyesters
#15
Veronika Perz, Altijana Hromic, Armin Baumschlager, Georg Steinkellner, Tea Pavkov-Keller, Karl Gruber, Klaus Bleymaier, Sabine Zitzenbacher, Armin Zankel, Claudia Mayrhofer, Carsten Sinkel, Ulf Kueper, Katharina Schlegel, Doris Ribitsch, Georg M Guebitz
Recently, a variety of biodegradable polymers have been developed as alternatives to recalcitrant materials. Although many studies on polyester biodegradability have focused on aerobic environments, there is much less known on biodegradation of polyesters in natural and artificial anaerobic habitats. Consequently, the potential of anaerobic biogas sludge to hydrolyze the synthetic compostable polyester PBAT (poly(butylene adipate-co-butylene terephthalate) was evaluated in this study. On the basis of reverse-phase high-performance liquid chromatography (RP-HPLC) analysis, accumulation of terephthalic acid (Ta) was observed in all anaerobic batches within the first 14 days...
March 15, 2016: Environmental Science & Technology
https://www.readbyqxmd.com/read/26652433/influence-of-low-contents-of-superhydrophilic-mwcnt-on-the-properties-and-cell-viability-of-electrospun-poly-butylene-adipate-co-terephthalate-fibers
#16
Bruno V M Rodrigues, Aline S Silva, Gabriela F S Melo, Luana M R Vasconscellos, Fernanda R Marciano, Anderson O Lobo
The use of poly (butylene adipate-co-terephthalate) (PBAT) in tissue engineering, more specifically in bone regeneration, has been underexplored to date due to its poor mechanical resistance. In order to overcome this drawback, this investigation presents an approach into the preparation of electrospun nanocomposite fibers from PBAT and low contents of superhydrophilic multi-walled carbon nanotubes (sMWCNT) (0.1-0.5wt.%) as reinforcing agent. We employed a wide range of characterization techniques to evaluate the properties of the resulting electrospun nanocomposites, including Field Emission Scanning Electronic Microscopy (FE-SEM), Transmission Electronic Microscopy (TEM), tensile tests, contact angle measurements (CA) and biological assays...
February 2016: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/26524601/hydrolysis-of-synthetic-polyesters-by-clostridium-botulinum-esterases
#17
Veronika Perz, Armin Baumschlager, Klaus Bleymaier, Sabine Zitzenbacher, Altijana Hromic, Georg Steinkellner, Andris Pairitsch, Andrzej Łyskowski, Karl Gruber, Carsten Sinkel, Ulf Küper, Doris Ribitsch, Georg M Guebitz
Two novel esterases from the anaerobe Clostridium botulinum ATCC 3502 (Cbotu_EstA and Cbotu_EstB) were expressed in Escherichia coli BL21-Gold(DE3) and were found to hydrolyze the polyester poly(butylene adipate-co-butylene terephthalate) (PBAT). The active site residues (triad Ser, Asp, His) are present in both enzymes at the same location only with some amino acid variations near the active site at the surrounding of aspartate. Yet, Cbotu_EstA showed higher kcat values on para-nitrophenyl butyrate and para-nitrophenyl acetate and was considerably more active (sixfold) on PBAT...
May 2016: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/26524205/new-pediatric-version-of-balloon-assisted-technique-for-atrial-septal-defect-closure-using-self-centering-devices-relation-to-interatrial-septal-thickness
#18
Sonia A El Saiedi, Wael A Attia
AIM: Some difficult atrial septal defect (ASD) cases with deficient rims or large defects may require specific maneuvers to facilitate transcatheter occlusion of these defects with self-centering devices. In our center, we developed a modification of balloon-assisted technique (BAT) for difficult ASDs to assist proper positioning of the device. Our aim was to demonstrate the efficiency of ASD closure with self-centering devices in children and to present the results of the new pediatric version of BAT (PBAT) in young children and its relation to the interatrial septal thickness...
November 2015: Journal of Invasive Cardiology
https://www.readbyqxmd.com/read/26490551/characterization-of-a-poly-butylene-adipate-co-terephthalate-hydrolyzing-lipase-from-pelosinus-fermentans
#19
Antonino Biundo, Altijana Hromic, Tea Pavkov-Keller, Karl Gruber, Felice Quartinello, Karolina Haernvall, Veronika Perz, Miriam S Arrell, Manfred Zinn, Doris Ribitsch, Georg M Guebitz
Certain α/β hydrolases have the ability to hydrolyze synthetic polyesters. While their partial hydrolysis has a potential for surface functionalization, complete hydrolysis allows recycling of valuable building blocks. Although knowledge about biodegradation of these materials is important regarding their fate in the environment, it is currently limited to aerobic organisms. A lipase from the anaerobic groundwater organism Pelosinus fermentans DSM 17108(PfL1) was cloned and expressed in Escherichia coli BL21-Gold (DE3) and purified from the cell extract...
February 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/26478395/non-leaching-antimicrobial-biodegradable-pbat-films-through-a-facile-and-novel-approach
#20
Dafu Wei, Hao Wang, Zainab Ziaee, Felipe Chibante, Anna Zheg, Huining Xiao
The antimicrobial thermoplastic starch (ATPS) containing guanidine-based polymers was obtained using a twin-screw extrusion with potato starch and polyhexamethylene guanidine hydrochloride (PHGH). Furthermore, the non-leaching antimicrobial biodegradable poly(butylene adipate-co-terephthalate) (PBAT) was prepared through reactive extrusion with PBAT and ATPS in the presence of the coupling agent, 2,2'-(1,3-phenylene)-bis (2-oxazoline) (PBO). Finally, the antimicrobial PBAT films were obtained by using a blown film extrusion system...
January 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
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