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https://www.readbyqxmd.com/read/29444588/periaortic-adipose-tissue-compared-with-peribrachial-adipose-tissue-mass-as-markers-and-possible-modulators-of-cardiometabolic-risk
#1
Elko Randrianarisoa, Norbert Stefan, Andreas Fritsche, Anja Hieronimus, Bernd Balletshofer, Jürgen Machann, Dorothea Siegel-Axel, Hans-Ulrich Häring, Kilian Rittig
Increased perivascular fat mass contributes to cardiometabolic risk (CMR). High peribrachial adipose tissue (PBAT) associates with insulin resistance independently of established CMR parameters. It is unknown to what extent periaortic adipose tissue (PAAT) may have a similar impact. In 95 participants, precise quantification of total adipose tissue, PBAT, PAAT, visceral adipose tissue (VAT), and liver fat (LF) content was performed by whole-body magnetic resonance imaging. Insulin sensitivity was determined by oral glucose tolerance test and carotid intima-media thickness (cIMT) by high-resolution ultrasound...
January 1, 2018: Angiology
https://www.readbyqxmd.com/read/29342118/microbial-degradation-behavior-in-seawater-of-polyester-blends-containing-poly-3-hydroxybutyrate-co-3-hydroxyhexanoate-phbhhx
#2
Hitoshi Sashiwa, Ryuji Fukuda, Tetsuo Okura, Shunsuke Sato, Atsuyoshi Nakayama
The microbial degradation behavior of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) and its compound with several polyesters such as poly(butylene adipate-co-telephtharate) (PBAT), poly(butylene succinate) (PBS), and polylactic acid (PLA) in seawater was tested by a biological oxygen demand (BOD) method. PHBHHx showed excellent biodegradation in seawater in this study. In addition, the biodegradation rate of several blends was much influenced by the weight ratio of PHBHHx in their blends and decreased in accordance with the decrement of PHBHHX ratio...
January 17, 2018: Marine Drugs
https://www.readbyqxmd.com/read/29342099/poly-lactic-acid-pla-based-tear-resistant-and-biodegradable-flexible-films-by-blown-film-extrusion
#3
Norma Mallegni, Thanh Vu Phuong, Maria-Beatrice Coltelli, Patrizia Cinelli, Andrea Lazzeri
Poly(lactic acid) (PLA) was melt mixed in a laboratory extruder with poly(butylene adipate-co-terephthalate) (PBAT) and poly(butylene succinate) (PBS) in the presence of polypropylene glycol di glycidyl ether (EJ400) that acted as both plasticizer and compatibilizer. The process was then scaled up in a semi-industrial extruder preparing pellets having different content of a nucleating agent (LAK). All of the formulations could be processed by blowing extrusion and the obtained films showed mechanical properties dependent on the LAK content...
January 17, 2018: Materials
https://www.readbyqxmd.com/read/29261293/biodegradable-pbat-pla-blend-with-bioactive-mcpa-phbv-conjugate-suppresses-weed-growth
#4
Iwona Kwiecień, Grazyna Adamus, Guozhan Jiang, Iza Radecka, Timothy C Baldwin, Habib R Khan, Brian Johnston, Valentina Pennetta, David Hill, Inna Bretz, Marek M Kowalczuk
The herbicide 2-methyl-4-chlorophenoxyacetic acid (MCPA) conjugated with poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was prepared via a melt transesterification route. The resultant bioactive oligomer was then mixed with a blend of polylactide (PLA) and poly(butylene adipate-co-terephthalate) (PBAT) with different loadings; to manufacture films to be used as a bioactive, biodegradable mulch to deliver the herbicide to target broadleaf weed species. The biological targeting of the MCPA-PHBV conjugate in the mulch film was investigated under glasshouse conditions using faba bean (Vicia faba) as a selective (non-target) model crop species having broadleaf morphology...
December 20, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/29224142/post-bisulfite-adaptor-tagging-for-pcr-free-whole-genome-bisulfite-sequencing
#5
Fumihito Miura, Takashi Ito
Post-bisulfite adaptor tagging (PBAT) is a highly efficient procedure to construct libraries for whole-genome bisulfite sequencing (WGBS). PBAT attaches adaptors to bisulfite-converted genomic DNA to circumvent bisulfite-induced degradation of library DNA inherent to conventional WGBS protocols. Consequently, it enables PCR-free WGBS from nanogram quantities of mammalian DNA, thereby serving as an invaluable tool for methylomics.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224140/whole-genome-bisulfite-sequencing-using-the-ovation%C3%A2-ultralow-methyl-seq-protocol
#6
Christian Daviaud, Victor Renault, Florence Mauger, Jean-François Deleuze, Jörg Tost
The analysis of genome-wide epigenomic alterations including DNA methylation has become a subject of intensive research for many complex diseases. Whole-genome bisulfite sequencing (WGBS) using next-generation sequencing technologies can be considered the gold standard for a comprehensive and quantitative analysis of cytosine methylation throughout the genome. Several approaches including tagmentation- and post bisulfite adaptor tagging (PBAT)-based WGBS have been devised. Here, we provide a detailed protocol based on a commercial kit for the preparation of libraries for WGBS from limited amounts of input DNA (50-100 ng) using the classical approach of WGBS by ligation of methylated adaptors to the fragmented DNA prior to bisulfite conversion...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29159827/family-based-tests-for-associating-haplotypes-with-general-phenotype-data-improving-the-fbat-haplotype-algorithm
#7
Julian Hecker, Xin Xu, F William Townes, Heide Loehlein Fier, Chris Corcoran, Nan Laird, Christoph Lange
For family-based association studies, Horvath et al. proposed an algorithm for the association analysis between haplotypes and arbitrary phenotypes when the phase of the haplotypes is unknown, that is, genotype data is given. Their approach to haplotype analysis maintains the original features of the TDT/FBAT-approach, that is, complete robustness against genetic confounding and misspecification of the phenotype. The algorithm has been implemented in the FBAT and PBAT software package and has been used in numerous substantive manuscripts...
November 21, 2017: Genetic Epidemiology
https://www.readbyqxmd.com/read/29073848/biodegradation-of-moringa-oleifera-s-polymer-blends
#8
Cristiane Medina Finzi-Quintão, Kátia Monteiro Novack, Ana Cláudia Bernardes-Silva, Thais D Silva, Lucas E S Moreira, Luiza E M Braga
Vegetable oils are used as base for synthesis of polymers and monomers with similar structures of petroleum, as plasticizers for conventional polymers and biodegrading additives. The M. oleifera oil was extract from seeds and polymerized after being submitted to 16 hours of microwave irradiation without catalyzers. This polymer was characterized and the efficiency of the oil polymerization was verified by the reducing of doubles bonds and the increasing of molecular weight up to 50,000 g.mol-1. Films produced by mixture of low density polyethylene (LDPE) with poly(butylene adipate-co-terephthalate)/poly(lactic acid) (PBAT/PLA) present low tensile resistance and low biodegradation behaviour...
October 26, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28956811/ionic-liquids-as-surfactants-for-layered-double-hydroxide-fillers-effect-on-the-final-properties-of-poly-butylene-adipate-co-terephthalate
#9
Sébastien Livi, Luanda Chaves Lins, Jakub Peter, Hynek Benes, Jana Kredatusova, Ricardo K Donato, Sébastien Pruvost
In this work, phosphonium ionic liquids (ILs) based on tetra-alkylphosphonium cations combined with carboxylate, phosphate and phosphinate anions, were used for organic modification of layered double hydroxide (LDH). Two different amounts (2 and 5 wt %) of the organically modified LDHs were mixed with poly(butylene adipate-co-terephthalate) (PBAT) matrix by melt extrusion. All prepared PBAT/IL-modified-LDH composites exhibited increased mechanical properties (20-50% Young's modulus increase), decreased water vapor permeability (30-50% permeability coefficient reduction), and slight decreased crystallinity (10-30%) compared to the neat PBAT...
September 28, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28732900/characterization-of-layered-silicate-reinforced-blends-of-thermoplastic-starch-tps-and-poly-butylene-adipate-co-terephthalate
#10
László Lendvai, Anton Apostolov, József Karger-Kocsis
A two-step melt blending procedure was used to produce binary systems composed of thermoplastic starch (TPS) and poly(butylene adipate-co-terephthalate) (PBAT). To improve the properties of the blends, two different layered silicates, viz. bentonite (BT) and organically modified montmorillonite (oMMT) were incorporated. First, TPS and its layered silicate nanocomposites were prepared via extrusion compounding during which starch was plasticized with glycerol and water. In the second step, PBAT was added to TPS/layered silicate to produce blends in a batch-type mixer...
October 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28616970/structure-and-biocompatibility-of-bioabsorbable-nanocomposites-of-aliphatic-aromatic-copolyester-and-cellulose-nanocrystals
#11
Shahab Kashani Rahimi, Robabeh Aeinehvand, Kyoungtae Kim, Joshua U Otaigbe
Poly(butylene adipate-co-terephthalate) (PBAT) was first chemically modified via free radical grafting with maleic anhydride (MA) and the MA-g-PBAT graft copolymer was then used as a matrix material to obtain cellulose nanocrystal (CNC)-reinforced MA-g-PBAT bionanocomposites via reactive extrusion process to accelerate efforts to develop functional bioabsorbable polymer nanocomposites with improved properties. The molecular structure of the PBAT after chemical modification with maleic anhydride was confirmed by (1)H NMR and FTIR spectroscopy...
June 27, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28602292/high-sensitive-bioorthogonal-sers-tag-for-live-cancer-cell-imaging-by-self-assembling-core-satellites-structure-gold-silver-nanocomposite
#12
Meng Chen, Ling Zhang, Mingxia Gao, Xiangmin Zhang
A novel, high-sensitivity, biocompatible SERS tag with core-shell structure based on gold nanoparticles containing alkynyl molecule core -silver nanoparticle satellites shell was fabricated for the first time to be used for live cancer cells Surface enhanced Raman scattering (SERS) imaging. (E)-2-((4-(phenylethynyl)benzylidene) amino) ethanethiol (PBAT) synthesized facilely in our lab is the Raman-silence region reporter which is advantage for bioorthogonal SERS cell imaging. In order to enhance the intensity of the Raman tags for live cancer cell imaging, a series of news measures have been adopted...
September 1, 2017: Talanta
https://www.readbyqxmd.com/read/28576069/starch-cellulose-acetate-and-polyester-biodegradable-sheets-effect-of-composition-and-processing-conditions
#13
Allan Robledo Fialho E Moraes, Cícero Cardoso Pola, Ana Paula Bilck, Fabio Yamashita, Jairo Tronto, Eber Antonio Alves Medeiros, Nilda de Fátima Ferreira Soares
The production of biodegradable plastic materials using natural resources has aroused increased attention due to environmental concerns. This study aimed to manufacture novel, commercially feasible, biodegradable sheets by flat die extrusion-calendering process produced with thermoplastic starch/plasticized cellulose acetate (TPS/PCA) and thermoplastic starch/plasticized cellulose acetate/poly (butylene adipate-co-terephthalate) (TPS/PCA/PBAT) blends, and to investigate the effects of composition and processing conditions, morphological characteristics, and thermal properties...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28548818/pla-pbat-bionanocomposites-with-antimicrobial-natural-rosin-for-green-packaging
#14
Hesham Moustafa, Nadia El Kissi, Ahmed I Abou-Kandil, Mohamed S Abdel-Aziz, Alain Dufresne
The use of biodegradable polymers is of great importance nowadays in many applications. Some of the most commonly used biopolymers are polylactic acid (PLA) and poly(butylene adipate-co-terephthalate) (PBAT) due to their superior properties and availability. In this manuscript, we use a facile and green modification method of organoclay (OC) by antimicrobial natural rosin which is considered as a toxicity-free reinforcing material, thus keeping the green character of the material. It increases the interlayer spacing between the clay platelets...
June 2, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28538100/enzymatic-hydrolysis-of-polyester-thin-films-at-the-nanoscale-effects-of-polyester-structure-and-enzyme-active-site-accessibility
#15
Michael Thomas Zumstein, Daniela Rechsteiner, Nicolas Roduner, Veronika Perz, Doris Ribitsch, Georg M Guebitz, Hans-Peter E Kohler, Kristopher McNeill, Michael Sander
Biodegradable polyesters have a large potential to replace persistent polymers in numerous applications and to thereby reduce the accumulation of plastics in the environment. Ester hydrolysis by extracellular carboxylesterases is considered the rate-limiting step in polyester biodegradation. In this work, we systematically investigated the effects of polyester and carboxylesterase structure on the hydrolysis of nanometer-thin polyester films using a quartz-crystal microbalance with dissipation monitoring. Hydrolyzability increased with increasing polyester-chain flexibility as evidenced from differences in the hydrolysis rates and extents of aliphatic polyesters varying in the length of their dicarboxylic acid unit and of poly(butylene adipate-co-terephthalate) (PBAT) polyesters varying in their terephthalate-to-adipate ratio by Rhizopus oryzae lipase and Fusarium solani cutinase...
June 13, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28520814/rs12970134-near-mc4r-is-associated-with-appetite-and-beverage-intake-in-overweight-and-obese-children-a-family-based-association-study-in-chinese-population
#16
Shuo Wang, Jieyun Song, Yide Yang, Nitesh V Chawla, Jun Ma, Haijun Wang
BACKGROUND: Recent studies indicated that eating behaviors are under genetic influence, and the melanocortin 4 receptor (MC4R) gene polymorphisms can affect the total energy intake and the consumption of fat, protein and carbohydrates. Our study aims at investigating the association of the MC4R polymorphism with appetite and food intake among Chinese children. METHODS: A family-based association study was conducted among 151 Chinese trios whose offsprings were overweight/obese children aged 9-15 years...
2017: PloS One
https://www.readbyqxmd.com/read/28494433/electrospun-ultrathin-pbat-nhap-fibers-influenced-the-in-vitro-and-in-vivo-osteogenesis-and-improved-the-mechanical-properties-of-neoformed-bone
#17
Gabriela F Santana-Melo, Bruno V M Rodrigues, Edmundo da Silva, Ritchelli Ricci, Fernanda R Marciano, Thomas J Webster, Luana M R Vasconcellos, Anderson O Lobo
Combining polyester scaffolds with synthetic nanohydroxyapatite (nHAp), which is bioactive and osteoconductive, is a plausible strategy to improve bone regeneration. Here, we propose the combination of PBAT [poly(butylene-adipate-co-terephthalate)] and synthetic nHAp (at 3 and 5wt%). PBAT is a relatively a new polymer with low crystallinity and attractive biodegradability and mechanical properties for orthopedic applications, however, with a still underexplored potential for in vivo applications. Then, we performed a careful biological in vitro and in vivo set of experiments to evaluate the influence of PBAT containing two different nHAp loads...
April 27, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28457463/effect-of-reprocessing-cycles-on-the-degradation-of-pp-pbat-thermoplastic-starch-blends
#18
Thainá A Oliveira, Rosimery R Oliveira, Renata Barbosa, Joyce B Azevedo, Tatianny S Alves
The solid waste management problems caused by the accumulation of plastics require measures to mitigate the environmental damage, and mechanical recycling of plastics is among the possible solutions. In this context, the present study aimed to evaluate the effects of mechanical recycling on the properties of a polypropylene/poly(butylene adipate co-terephthalate)-thermoplastic starch blend (PP/PBAT-Thermoplastic starch blend) when it was subjected to seven reprocessing cycles by a single-screw extruder. The observations by infrared spectroscopy indicated that the chemical structures of the blend and the polypropylene matrix did not present significant changes with the reprocessing cycles...
July 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28352895/partial-to-complete-wetting-transitions-in-immiscible-ternary-blends-with-pla-the-influence-of-interfacial-confinement
#19
Ali M Zolali, Basil D Favis
In this study it is shown that the three different intermediate phases in melt blended ternary PLA/PHBV/PBS, PLA/PBAT/PE and PLA/PE/PBAT systems all demonstrate partial wetting, but have very different wetting behaviors as a function of composition and annealing. The interfacial tension of the various components, their spreading coefficients and the contact angles of the confined partially wet droplets at the interface are examined in detail. A wetting transition from partially wet droplets to a complete layer at the interface is observed for both PHBV and PBAT by increasing the concentration and also by annealing...
April 12, 2017: Soft Matter
https://www.readbyqxmd.com/read/28252280/interfacial-shish-kebabs-lengthened-by-coupling-effect-of-in-situ-flexible-nanofibrils-and-intense-shear-flow-achieving-hierarchy-to-conquer-the-conflicts-between-strength-and-toughness-of-polylactide
#20
Sheng-Yang Zhou, Ben Niu, Xu-Long Xie, Xu Ji, Gan-Ji Zhong, Benjamin S Hsiao, Zhong-Ming Li
The challenge of hitherto elaborating a feasible pathway to overcome the conflicts between strength and toughness of polylactide (PLA) still remains among academia and industry. In the current work, a unique hierarchal structure of flexible poly(butylene adipate-co-terephthalate) (PBAT) in situ nanofibrils integrating with abundant PLA shish-kebabs as a strong building block was disclosed and expresses its capability to conquer this dilemma. Substantially simultaneous enhancement on tensile strength, impact strength, and elongation at break could be achieved up to 91...
March 22, 2017: ACS Applied Materials & Interfaces
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