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https://www.readbyqxmd.com/read/28723492/validation-of-gene-expression-signatures-to-identify-low-risk-clear-cell-renal-cell-carcinoma-patients-at-higher-risk-for-disease-related-death
#1
Mansi Parasramka, Daniel J Serie, Yan W Asmann, Jeanette E Eckel-Passow, Erik P Castle, Melissa L Stanton, Brad C Leibovich, Robert Houston Thompson, E Aubrey Thompson, Alexander S Parker, Thai H Ho, Richard W Joseph
BACKGROUND: Approximately 5-10% of patients with "low-risk" clear cell renal cell carcinoma (ccRCC), as stratified by externally validated clinicopathologic prognostic algorithms, eventually have disease relapse and die. Improving prognostic algorithms for these low-risk patients could help to provide improved individualized surveillance recommendations. OBJECTIVE: To identify genes that are differentially expressed in patients with low-risk ccRCC who did and did not die of their disease...
December 15, 2016: European Urology Focus
https://www.readbyqxmd.com/read/28684614/ubiquinol-effects-on-antiphospholipid-syndrome-prothrombotic-profile-a-randomized-placebo-controlled-trial
#2
Carlos Pérez-Sánchez, María Ángeles Aguirre, Patricia Ruiz-Limón, María Carmen Abalos-Aguilera, Yolanda Jiménez-Gómez, Iván Arias-de la Rosa, Antonio Rodriguez-Ariza, Lucía Fernández-Del Río, José Antonio Gonzalez-Reyes, Pedro Segui, Eduardo Collantes-Estévez, Nuria Barbarroja, Francisco Velasco, Savino Sciascia, Irene Cecchi, María José Cuadrado, José Manuel Villalba, Chary López-Pedrera
OBJECTIVE: Antiphospholipid syndrome (APS) leukocytes exhibit an oxidative perturbation, directly linked to alterations in mitochondrial dynamics and metabolism. This disturbance is related to the patients' prothrombotic status and can be prevented by in vitro treatment with coenzyme Q10. Our aim was to investigate short-term effects of in vivo ubiquinol (reduced coenzyme Q10[Qred]) supplementation on markers related to inflammation and thrombosis in APS through a prospective, randomized, crossover, placebo-controlled trial...
July 6, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28679778/combination-therapy-with-nhs-muil12-and-avelumab-anti-pd-l1-enhances-antitumor-efficacy-in-preclinical-cancer-models
#3
Chunxiao Xu, Yanping Zhang, P Alexander Rolfe, Vivian M Hernández, Wilson Guzman, Giorgio Kradjian, Bo Marelli, Guozhong Qin, Jin Qi, Hong Wang, Huakui Yu, Robert Tighe, Kin-Ming Lo, Jessie M English, Laszlo G Radvanyi, Yan Lan
To determine if combination therapy with NHS-muIL12 and the anti-PD-L1 antibody avelumab can enhance anti-tumor efficacy in preclinical models relative to monotherapies.<br /><br />Experimental Design: BALB/c mice bearing orthotopic EMT-6 mammary tumors and μMt(-) mice bearing subcutaneous MC38 tumors were treated with NHS-muIL12, avelumab, or combination therapy; tumor growth and survival were assessed. Tumor recurrence following remission and rechallenge was evaluated in EMT-6 tumor-bearing mice...
July 5, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28679352/identification-of-two-distinct-molecular-subtypes-by-digital-rna-counting-of-non-invasive-follicular-tumour-with-papillary-like-nuclear-features-niftp
#4
Riccardo Giannini, Clara Ugolini, Anello Marcello Poma, Maria Urpì, Cristina Niccoli, Rossella Elisei, Massimo Chiarugi, Paolo Vitti, Paolo Miccoli, Fulvio Basolo
Backgound. The follicular variant (FV) is one of the most common variants of papillary thyroid carcinoma (PTC). Clinically, the FVPTC is considered a low-risk variant of PTC, and the encapsulated forms of FVPTC represent a group of thyroid tumours with an overall good prognosis. Consequently, these neoplasms were very recently reclassified as "non-invasive follicular tumour with papillary-like nuclear features (NIFTP)". From a molecular standpoint, NIFTP appears similar to the follicular pattern thyroid neoplasm; however, limited data are currently available regarding their gene expression profile...
July 5, 2017: Thyroid: Official Journal of the American Thyroid Association
https://www.readbyqxmd.com/read/28668836/microrna-profiling-and-target-genes-related-to-metastasis-of-salivary-adenoid-cystic-carcinoma
#5
Xin Feng, Kyle Matsuo, Tan Zhang, Yunping Hu, Ashley C Mays, James D Browne, Xiaobo Zhou, Christopher A Sullivan
BACKGROUND/AIM: Perineural invasion and distant metastasis lead to a poor prognosis of adenoid cystic carcinoma and there is no effective therapy available. MicroRNAs (miRNAs) are small non-coding RNAs that regulate target gene expression, which can be biomarkers or therapeutic targets for certain cancer types. We aimed to identify miRNAs and their target genes possibly involved in metastasis of salivary gland adenoid cystic carcinoma (SACC). MATERIALS AND METHODS: Using Nanostring nCounter analysis, we examined miRNA expression in two SACC cell lines: SACC-83 and SACC-LM, with low and high lung metastasis rates, respectively...
July 2017: Anticancer Research
https://www.readbyqxmd.com/read/28668567/10-valent-pneumococcal-conjugate-vaccine-pcv10-decreases-metabolic-activity-but-not-nasopharyngeal-carriage-of-streptococcus-pneumoniae-and-haemophilus-influenzae
#6
Dafne C Andrade, Igor C Borges, Maiara L Bouzas, Juliana R Oliveira, Kiyoshi F Fukutani, Artur T Queiroz, Camila Indiani de Oliveira, Aldina Barral, Johan Van Weyenbergh, Cristiana Nascimento-Carvalho
BACKGROUND: The effect of pneumococcal vaccination is widely variable when measured by nasopharyngeal carriage of vaccine and non-vaccine targets. The aim of this study was to compare the carriage rates and metabolic activity of Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae and Moraxella catarrhalis among children who were or were not vaccinated with PCV10. METHODS: We included children with acute respiratory infection aged 6-23months from a cross-sectional study (CHIADO-IVAS)...
June 28, 2017: Vaccine
https://www.readbyqxmd.com/read/28662087/unique-intrahepatic-transcriptomics-profiles-discriminate-the-clinical-phases-of-a-chronic-hbv-infection
#7
Jun Hou, Willem P Brouwer, Kim Kreefft, Lucio Gama, Sarah L Price, Harry L A Janssen, Pim J French, Thomas Vanwolleghem, Andre Boonstra
Chronic hepatitis B is a highly heterogeneous liver disease characterized by phases with fluctuations in viral replication and progressive liver damage in some, but not all infected individuals. Despite four decades of research, insight into host determinants underlying these distinct clinical phases-immunotolerant, immune active, inactive carrier, and HBeAg-negative hepatitis-remains elusive. We performed an in-depth transcriptome analysis of archived FFPE liver biopsies of each clinical phase to address host determinants associated with the natural history...
2017: PloS One
https://www.readbyqxmd.com/read/28658612/senescence-associated-micrornas-target-cell-cycle-regulatory-genes-in-normal-human-lung-fibroblasts
#8
Georgios S Markopoulos, Eugenia Roupakia, Maria Tokamani, George Vartholomatos, Theodore Tzavaras, Maria Hatziapostolou, Frank O Fackelmayer, Raphael Sandaltzopoulos, Christos Polytarchou, Evangelos Kolettas
Senescence recapitulates the ageing process at the cell level. A senescent cell stops dividing and exits the cell cycle. MicroRNAs (miRNAs) acting as master regulators of transcription, have been implicated in senescence. In the current study we investigated and compared the expression of miRNAs in young versus senescent human fibroblasts (HDFs), and analysed the role of mRNAs expressed in replicative senescent HFL-1 HDFs. Cell cycle analysis confirmed that HDFs accumulated in G1/S cell cycle phase. Nanostring analysis of isolated miRNAs from young and senescent HFL-1 showed that a distinct set of 15 miRNAs were significantly up-regulated in senescent cells including hsa-let-7d-5p, hsa-let-7e-5p, hsa-miR-23a-3p, hsa-miR-34a-5p, hsa-miR-122-5p, hsa-miR-125a-3p, hsa-miR-125a-5p, hsa-miR-125b-5p, hsa-miR-181a-5p, hsa-miR-221-3p, hsa-miR-222-3p, hsa-miR-503-5p, hsa-miR-574-3p, hsa-miR-574-5p and hsa-miR-4454...
June 27, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/28643426/cell-of-origin-classification-by-gene-expression-and-myc-rearrangements-in-diffuse-large-b-cell-lymphoma-of-children-and-adolescents
#9
Monika Szczepanowski, Jonas Lange, Christian W Kohler, Neus Masque-Soler, Martin Zimmermann, Sietse M Aukema, Michael Altenbuchinger, Thorsten Rehberg, Friederike Mahn, Reiner Siebert, Rainer Spang, Birgit Burkhardt, Wolfram Klapper
We present the largest series of diffuse large B-cell lymphoma (DLBCL) in patients younger than 18 years analysed to date by gene expression profiling using Nanostring technology to identify molecular subtypes and fluorescent in situ hybridization for translocations of MYC. We show that the activated B cell-like subtype of DLBCL is exceedingly rare in children and - in contrast to adults- not associated with outcome. Furthermore, we review the current literature and demonstrate that MYC translocations are not more frequent in paediatric compared to adult DLBCL...
June 23, 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/28640876/reliability-and-performance-of-commercial-rna-and-dna-extraction-kits-for-ffpe-tissue-cores
#10
Palak G Patel, Shamini Selvarajah, Karl-Philippe Guérard, John M S Bartlett, Jacques Lapointe, David M Berman, John B A Okello, Paul C Park
Cancer biomarker studies often require nucleic acid extraction from limited amounts of formalin-fixed, paraffin-embedded (FFPE) tissues, such as histologic sections or needle cores. A major challenge is low quantity and quality of extracted nucleic acids, which can limit our ability to perform genetic analyses, and have a significant influence on overall study design. This study was aimed at identifying the most reliable and reproducible method of obtaining sufficient high-quality nucleic acids from FFPE tissues...
2017: PloS One
https://www.readbyqxmd.com/read/28634582/cancer-stem-cells-in-moderately-differentiated-lip-squamous-cell-carcinoma-express-components-of-the-renin-angiotensin-system
#11
Rachna S Ram, Helen D Brasch, Jonathan C Dunne, Paul F Davis, Swee T Tan, Tinte Itinteang
AIM: We investigated the expression of the renin-angiotensin system (RAS) by cancer stem cell (CSC) subpopulations we have identified in moderately differentiated lip squamous cell carcinoma (MDLSCC). METHOD: Ten MDLSCC samples underwent 3,3-diaminobenzidine (DAB) and immunofluorescent immunohistochemical (IHC) staining for (pro)renin receptor (PRR), angiotensin-converting enzyme (ACE), angiotensin II receptor 1 (ATIIR1), and receptor 2 (ATIIR2). NanoString analysis and Western blotting (WB) were performed on six MDLSCC samples for gene and protein expression, respectively...
2017: Frontiers in Surgery
https://www.readbyqxmd.com/read/28632792/identification-of-cell-proliferation-immune-response-and-cell-migration-as-critical-pathways-in-a-prognostic-signature-for-her2-er%C3%AE-breast-cancer
#12
Jeffrey C Liu, Miriam Zacksenhouse, Andrea Eisen, Sharon Nofech-Mozes, Eldad Zacksenhaus
BACKGROUND: Multi-gene prognostic signatures derived from primary tumor biopsies can guide clinicians in designing an appropriate course of treatment. Identifying genes and pathways most essential to a signature performance may facilitate clinical application, provide insights into cancer progression, and uncover potentially new therapeutic targets. We previously developed a 17-gene prognostic signature (HTICS) for HER2+:ERα- breast cancer patients, using genes that are differentially expressed in tumor initiating cells (TICs) versus non-TICs from MMTV-Her2/neu mammary tumors...
2017: PloS One
https://www.readbyqxmd.com/read/28624625/a-formalin-fixed-paraffin-embedded-ffpe-based-prognostic-signature-to-predict-metastasis-in-clinically-low-risk-stage-i-ii-microsatellite-stable-colorectal-cancer
#13
Yee Syuen Low, Christopher Blöcker, John R McPherson, See Aik Tang, Ying Ying Cheng, Joyner Y S Wong, Clarinda Chua, Tony K H Lim, Choong Leong Tang, Min Hoe Chew, Patrick Tan, Iain B Tan, Steven G Rozen, Peh Yean Cheah
Approximately 20% early-stage (I/II) colorectal cancer (CRC) patients develop metastases despite curative surgery. We aim to develop a formalin-fixed and paraffin-embedded (FFPE)-based predictor of metastases in early-stage, clinically-defined low risk, microsatellite-stable (MSS) CRC patients. We considered genome-wide mRNA and miRNA expression and mutation status of 20 genes assayed in 150 fresh-frozen tumours with known metastasis status. We selected 193 genes for further analysis using NanoString nCounter arrays on corresponding FFPE tumours...
June 15, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28615018/the-aquaglyceroporin-aqp9-contributes-to-the-sex-specific-effects-of-in-utero-arsenic-exposure-on-placental-gene-expression
#14
Emily F Winterbottom, Devin C Koestler, Dennis Liang Fei, Eric Wika, Anthony J Capobianco, Carmen J Marsit, Margaret R Karagas, David J Robbins
BACKGROUND: Sex-specific factors play a major role in human health and disease, including responses to environmental stresses such as toxicant exposure. Increasing evidence suggests that such sex differences also exist during fetal development. In a previous report using the resources of the New Hampshire Birth Cohort Study (NHBCS), we found that low-to-moderate in utero exposure to arsenic, a highly toxic and widespread pollutant, was associated with altered expression of several key developmental genes in the fetal portion of the placenta...
June 14, 2017: Environmental Health: a Global Access Science Source
https://www.readbyqxmd.com/read/28611989/expression-of-cathepsins-b-d-and-g-in-isocitrate-dehydrogenase-wildtype-glioblastoma
#15
Sabrina P Koh, Agadha C Wickremesekera, Helen D Brasch, Reginald Marsh, Swee T Tan, Tinte Itinteang
AIM: To investigate the expression of cathepsins B, D, and G, in relation to the cancer stem cell (CSC) subpopulations, we have previously characterized within isocitrate dehydogenase (IDH)-wildtype glioblastoma (IDHWGB). METHODS: 3,3-Diaminobezidine (DAB) immunohistochemical (IHC) staining for cathepsins B, D, and G, was performed on 4μm-thick formalin-fixed paraffin-embedded IDHWGB samples obtained from six patients. Two representative DHWGB samples from the original cohort of patients were selected for immunofluorescent (IF) IHC staining, to identify the localization of the cathepsins in relation to the CSC subpopulations...
2017: Frontiers in Surgery
https://www.readbyqxmd.com/read/28600498/deregulation-of-mirnas-in-malignant-pleural-mesothelioma-is-associated-with-prognosis-and-suggests-an-alteration-of-cell-metabolism
#16
Chiara De Santi, Ombretta Melaiu, Alessandra Bonotti, Luciano Cascione, Gianpiero Di Leva, Rudy Foddis, Alfonso Cristaudo, Marco Lucchi, Marco Mora, Anna Truini, Andrea Tironi, Bruno Murer, Renzo Boldorini, Monica Cipollini, Federica Gemignani, Pierluigi Gasparini, Luciano Mutti, Stefano Landi
Malignant pleural mesothelioma (MPM) is an aggressive human cancer and miRNAs can play a key role for this disease. In order to broaden the knowledge in this field, the miRNA expression was investigated in a large series of MPM to discover new pathways helpful in diagnosis, prognosis and therapy. We employed nanoString nCounter system for miRNA profiling on 105 MPM samples and 10 healthy pleura. The analysis was followed by the validation of the most significantly deregulated miRNAs by RT-qPCR in an independent sample set...
June 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28596502/correlation-between-mek-signature-and-ras-gene-alteration-in-advanced-gastric-cancer
#17
Soomin Ahn, Roz Brant, Alan Sharpe, Jonathan R Dry, Darren R Hodgson, Elaine Kilgour, Kyung Kim, Seung Tae Kim, Se Hoon Park, Won Ki Kang, Kyoung-Mee Kim, Jeeyun Lee
MEK inhibitor (selumetinib) is a potent, orally active inhibitor of MAPK/ERK pathway. It is important to develop an accurate and robust method indicative of RAS pathway activity to stratify potential patients who can benefit from selumetinib treatment in gastric cancer (GC). First, we surveyed the sensitivity to selumetinib in a panel of 22 GC cell lines and correlated with MEK signature to selumetinib sensitivity. Next, we analyzed MEK signature via nanostring assay in two Asian cohorts using clinical samples (n = 218) and then performed a correlative analysis with MEK signature status and KRAS genotype in GC...
May 23, 2017: Oncotarget
https://www.readbyqxmd.com/read/28575868/increased-breast-tissue-receptor-activator-of-nuclear-factor-%C3%AE%C2%BAb-ligand-rankl-gene-expression-is-associated-with-higher-mammographic-density-in-premenopausal-women
#18
Adetunji T Toriola, Ha X Dang, Ian S Hagemann, Catherine M Appleton, Graham A Colditz, Jingqin Luo, Christopher A Maher
Increased mammographic breast density is associated with a 4-6-fold increased risk of breast cancer, yet lifestyle factors that can reduce dense breasts are yet to be identified, and viable prevention strategies to reduce breast density-associated breast cancer development are yet to be developed. We investigated the associations of breast tissue receptor activator of nuclear factor-κB (RANK) pathway gene expression with mammographic density in 48 premenopausal women, with no previous history of cancer. Gene expression levels were measured in total RNA isolated from formalin-fixed paraffin-embedded breast tissue samples, using the NanoString nCounter platform...
May 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/28572860/pulmonary-microrna-profiling-implications-in-upper-lobe-predominant-lung-disease
#19
David A Armstrong, Amanda B Nymon, Carol S Ringelberg, Corina Lesseur, Haley F Hazlett, Louisa Howard, Carmen J Marsit, Alix Ashare
BACKGROUND: Numerous pulmonary diseases manifest with upper lobe predominance including cystic fibrosis, smoking-related chronic obstructive pulmonary disease, and tuberculosis. Zonal hypoxia, characteristic of these pulmonary maladies, and oxygen stress in general is known to exert profound effects on various important aspects of cell biology. Lung macrophages are major participants in the pulmonary innate immune response and regional differences in macrophage responsiveness to hypoxia may contribute in the development of lung disease...
2017: Clinical Epigenetics
https://www.readbyqxmd.com/read/28555079/muc1-inhibition-leads-to-decrease-in-pd-l1-levels-via-up-regulation-of-mirnas
#20
A R Pyzer, D Stroopinsky, J Rosenblatt, E Anastasiadou, H Rajabi, A Washington, A Tagde, J-H Chu, M Coll, A L Jiao, L T Tsai, D E Tenen, L Cole, K Palmer, A Ephraim, R K Leaf, M Nahas, A Apel, Mb-N Zommer, S Jain, M McMasters, L Mendez, J Arnason, B A Raby, F Slack, D Kufe, D Avigan
The PD-L1/PD-1 pathway is a critical component of the immunosuppressive tumor microenvironment in AML, but little is known about its regulation. We investigated the role of the MUC1 oncoprotein in modulating PD-L1 expression in AML. Silencing of MUC1 in AML cell lines suppressed PD-L1 expression without a decrease in PD-L1 mRNA levels, suggesting a post-transcriptional mechanism of regulation. We identified the microRNAs miR-200c and miR-34a as key regulators of PD-L1 expression in AML. Silencing of MUC1 in AML cells led to a marked increase in miR-200c and miR-34a levels, without changes in precursor microRNA, suggesting that MUC1 might regulate microRNA processing...
May 30, 2017: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
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