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https://www.readbyqxmd.com/read/29761864/high-throughput-detection-and-screening-of-plantsmodified-by-geneediting-using-quantitative-real-time-pcr
#1
Cheng Peng, Hua Wang, Xiaoli Xu, Xiaofu Wang, Xiaoyun Chen, Wei Wei, Yongmin Lai, Guoquan Liu, Godwin Ian, Jieqin Li, Ling Zhang, Junfeng Xu
Gene editing techniques are becoming powerful tools for modifying target genes in organisms. Although several methods have been developed to detect gene-edited organisms, these techniques are time- and labour-intensive. Meanwhile, few studies have investigated high-throughput detection and screening strategies for plants modified by gene editing. In this study, we developed a simple, sensitive and high-throughput quantitative real-time(qPCR)-based method. The qPCR-based methodexploits two differently labelled probes that are placed within one amplicon at the gene editing target site to simultaneously detect the wild type and a gene-edited mutant...
May 15, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29751299/net-ecosystem-exchange-of-co-2-and-h-2-o-fluxes-from-irrigated-grain-sorghum-and-maize-in-the-texas-high-plains
#2
Pradeep Wagle, Prasanna H Gowda, Jerry E Moorhead, Gary W Marek, David K Brauer
Net ecosystem exchange (NEE) of carbon dioxide (CO2 ) and water vapor (H2 O) fluxes from irrigated grain sorghum (Sorghum bicolor L. Moench) and maize (Zea mays L.) fields in the Texas High Plains were quantified using the eddy covariance (EC) technique during 2014-2016 growing seasons and examined in terms of relevant controlling climatic variables. Eddy covariance measured evapotranspiration (ETEC ) was also compared against lysimeter measured ET (ETLys ). Daily peak (7-day averages) NEE reached approximately -12 g C m-2 for sorghum and -14...
May 8, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29723054/coupling-of-bioaugmentation-and-phytoremediation-to-improve-pcbs-removal-from-a-transformer-oil-contaminated-soil
#3
Maryam Salimizadeh, Mehran Shirvani, Hossein Shariatmadari, Mahnaz Nikaeen, Seyedeh Leili Mohebi Nozar
This study was carried out to assess the dissipation of 17 selected polychlorinated biphenyl (PCBi ) congeners in a transformer oil-contaminated soil using bioaugmentation with 2 PCB-degrading bacterial strains, i.e., Pseudomonas spp. S5 and Alcaligenes faecalis, assisted or not by the maize (Zea mays L.) plantation. After 5 and 10 weeks of treatment, the remaining concentrations of the target PCBi congeners in the soil were extracted and measured using GC-MS. Results showed that the bacterial augmentation treatments with Pseudomonas spp...
June 7, 2018: International Journal of Phytoremediation
https://www.readbyqxmd.com/read/29678859/gsyellow-a-swiss-knife-tag-for-functional-protein-analysis-in-monocot-and-dicot-plants
#4
Nienke Besbrugge, Jelle Van Leene, Dominique Eeckhout, Bernard Cannoot, Shubhada R Kulkarni, Nancy De Winne, Geert Persiau, Eveline Van De Slijke, Michiel Bontinck, Stijn Aesaert, Francis Impens, Kris Gevaert, Daniel van Damme, Mieke Van Lijsebettens, Dirk Inze, Klaas Vandepoele, Hilde Nelissen, Geert De Jaeger
The ability to tag proteins has boosted the emergence of generic molecular methods for protein functional analysis. Fluorescent protein tags are used to visualize protein localization and affinity tags enable the mapping of molecular interactions by, for example, tandem affinity purification (TAP) or chromatin immunoprecipitation (ChIP). To apply these widely used molecular techniques on a single transgenic plant line, we developed a multifunctional TAP tag, named GSyellow, which combines the streptavidin-binding peptide (SBP) tag with citrine yellow fluorescent protein (cYFP)...
April 20, 2018: Plant Physiology
https://www.readbyqxmd.com/read/29597324/single-kernel-ft-nir-spectroscopy-for-detecting-supersweet-corn-zea-mays-l-saccharata-sturt-seed-viability-with-multivariate-data-analysis
#5
Guangjun Qiu, Enli Lü, Huazhong Lu, Sai Xu, Fanguo Zeng, Qin Shui
The viability and vigor of crop seeds are crucial indicators for evaluating seed quality, and high-quality seeds can increase agricultural yield. The conventional methods for assessing seed viability are time consuming, destructive, and labor intensive. Therefore, a rapid and nondestructive technique for testing seed viability has great potential benefits for agriculture. In this study, single-kernel Fourier transform near-infrared (FT-NIR) spectroscopy with a wavelength range of 1000-2500 nm was used to distinguish viable and nonviable supersweet corn seeds...
March 28, 2018: Sensors
https://www.readbyqxmd.com/read/29534439/preparation-of-well-dispersed-chitosan-alginate-hollow-multilayered-microcapsules-for-enhanced-cellular-internalization
#6
Carla Ribeiro, João Borges, Ana M S Costa, Vítor M Gaspar, Verónica de Zea Bermudez, João F Mano
Hollow multilayered capsules have shown massive potential for being used in the biomedical and biotechnology fields, in applications such as cellular internalization, intracellular trafficking, drug delivery, or tissue engineering. In particular, hollow microcapsules, developed by resorting to porous calcium carbonate sacrificial templates, natural-origin building blocks and the prominent Layer-by-Layer (LbL) technology, have attracted increasing attention owing to their key features. However, these microcapsules revealed a great tendency to aggregate, which represents a major hurdle when aiming for cellular internalization and intracellular therapeutics delivery...
March 10, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29496777/loci-that-control-nonlinear-interdependent-responses-to-combinations-of-drought-and-nitrogen-limitation
#7
Megan M Chang, Danielle Allery Nail, Toni Kazic, Susan J Simmons, Ann E Stapleton
Crop improvement must accelerate to feed an increasing human population in the face of environmental changes. Including anticipated climatic changes with genetic architecture in breeding programs could better optimize improvement strategies. Combinations of drought and nitrogen limitation already occur world-wide. We therefore analyzed the genetic architecture underlying the response of Zea mays to combinations of water and nitrogen stresses. Recombinant inbreds were subjected to nine combinations of the two stresses using an optimized response surface design, and their growth was measured...
May 4, 2018: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29461798/detection-and-identification-of-plant-pathogens-on-maize-kernels-with-a-hand-held-raman-spectrometer
#8
Charles Farber, Dmitry Kurouski
Rapid detection and identification of crop pathogens is essential for improving crop yield. Typical pathogen assaying methods, such as polymerase chain reaction (PCR) or enzyme-linked immunosorbent assay (ELISA), are time-consuming and destructive to the sample. Raman spectroscopy (RS) is a noninvasive nondestructive analytical technique that provides insight on the chemical structure of the specimen. In this study, we demonstrate that using a hand-held Raman spectrometer, in combination with chemometric analyses, we can distinguish between healthy and diseased maize ( Zea mays) kernels, as well as between different diseases with 100% accuracy...
March 6, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29455282/molecularly-imprinted-vs-reversed-phase-extraction-for-the-determination-of-zearalenone-a-method-development-and-critical-comparison-of-sample-clean-up-efficiency-achieved-in-an-on-line-coupled-spe-chromatography-system
#9
Ivona Lhotská, Barbora Gajdošová, Petr Solich, Dalibor Šatínský
Sample preparation prior to chromatographic separation plays an important role in the analytical process. To avoid time-consuming and manual handling sample-prep, automated on-line techniques such as on-line SPE-HPLC are therefore preferred. In this study, two different on-line extraction approaches for mycotoxin/endocrine disruptor zearalenone (ZEA) determination using either molecularly imprinted polymer (MIP) with selective cavities and binding sites for extraction or a reversed-phase sorbent C18 providing non-selective interactions have been developed, validated, and compared...
May 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29444064/optimized-random-phase-only-holograms
#10
Alejandro Velez Zea, John Fredy Barrera Ramirez, Roberto Torroba
We propose a simple and efficient technique capable of generating Fourier phase only holograms with a reconstruction quality similar to the results obtained with the Gerchberg-Saxton (G-S) algorithm. Our proposal is to use the traditional G-S algorithm to optimize a random phase pattern for the resolution, pixel size, and target size of the general optical system without any specific amplitude data. This produces an optimized random phase (ORAP), which is used for fast generation of phase only holograms of arbitrary amplitude targets...
February 15, 2018: Optics Letters
https://www.readbyqxmd.com/read/29392581/a-novel-patient-derived-xenograft-model-for-claudin-low-triple-negative-breast-cancer
#11
Margarite D Matossian, Hope E Burks, Annie C Bowles, Steven Elliott, Van T Hoang, Rachel A Sabol, Nicholas C Pashos, Benjamen O'Donnell, Kristin S Miller, Bahia M Wahba, Bruce A Bunnell, Krzysztof Moroz, Arnold H Zea, Steven D Jones, Augusto C Ochoa, Amir A Al-Khami, Fokhrul Hossain, Adam I Riker, Lyndsay V Rhodes, Elizabeth C Martin, Lucio Miele, Matthew E Burow, Bridgette M Collins-Burow
BACKGROUND: Triple-negative breast cancer (TNBC) subtypes are clinically aggressive and cannot be treated with targeted therapeutics commonly used in other breast cancer subtypes. The claudin-low (CL) molecular subtype of TNBC has high rates of metastases, chemoresistance and recurrence. There exists an urgent need to identify novel therapeutic targets in TNBC; however, existing models utilized in target discovery research are limited. Patient-derived xenograft (PDX) models have emerged as superior models for target discovery experiments because they recapitulate features of patient tumors that are limited by cell-line derived xenograft methods...
February 1, 2018: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/29368148/the-determination-of-zearalenone-and-its-major-metabolites-in-endometrial-cancer-tissues
#12
Martyna Pajewska, Mariusz Łojko, Krzysztof Cendrowski, Włodzimierz Sawicki, Tomasz Kowalkowski, Bogusław Buszewski, Renata Gadzała-Kopciuch
Endometrial cancer is one of the most commonly diagnosed cancers in women. The search for factors that contribute to the development of cancer cells in reproductive organs should involve the detection of xenoestrogens, in particular zearalenone (ZEA) and its metabolites. Xenoestrogens are endocrine disruptors-ZEA and its metabolites are structurally similar to estrogens (macrocyclic lactone ring) and show high affinity for estrogen receptors. This study proposes a new method for the preparation of samples of human tissues with endometrial cancer by the use of the QuEChERS technique...
January 24, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29303977/novel-chemical-process-for-producing-chrome-coated-metal
#13
Christopher Pelar, Karima Greenaway, Hugo Zea, Chun-Hsien Wu, Claudia C Luhrs, Jonathan Phillips
This work demonstrates that a version of the Reduction Expansion Synthesis (RES) process, Cr-RES, can create a micron scale Cr coating on an iron wire. The process involves three steps. I. A paste consisting of a physical mix of urea, chrome nitrate or chrome oxide, and water is prepared. II. An iron wire is coated by dipping. III. The coated, and dried, wire is heated to ~800 °C for 10 min in a tube furnace under a slow flow of nitrogen gas. The processed wires were then polished and characterized, primarily with scanning electron microscopy (SEM)...
January 5, 2018: Materials
https://www.readbyqxmd.com/read/29240921/cry1-bt-susceptibilities-of-fall-armyworm-lepidoptera-noctuidae-host-strains
#14
David A Ingber, Charles E Mason, Lindsey Flexner
The fall armyworm, Spodoptera frugiperda (Smith; Lepidoptera: Noctuidae), is a highly polyphagous, multivoltine pest of commercial crops including corn (Zea mays L.), cotton (Gossypium spp. L.), rice (Oryza sativa L.), and pasture grasses. Fall armyworm has become a growing concern in agricultural communities across the Americas as field populations in many locales have evolved resistance to several Cry1 toxins derived from the bacterium Bacillus thuringiensis Berliner (Bt). An often overlooked aspect of fall armyworm biology is the existence of two host strains, the 'rice' and 'corn' strains...
February 9, 2018: Journal of Economic Entomology
https://www.readbyqxmd.com/read/29083901/snapshot-transient-absorption-spectroscopy-of-carotenoid-radical-cations-in-high-light-acclimating-thylakoid-membranes
#15
Soomin Park, Alexandra L Fischer, Zhirong Li, Roberto Bassi, Krishna K Niyogi, Graham R Fleming
Nonphotochemical quenching mechanisms regulate light harvesting in oxygenic photosynthesis. Measurement techniques for nonphotochemical quenching have typically focused on downstream effects of quenching, such as measuring reduced chlorophyll fluorescence. Here, to directly measure a species involved in quenching, we report snapshot transient absorption (TA) spectroscopy, which rapidly tracks carotenoid radical cation signals as samples acclimate to excess light. The formation of zeaxanthin radical cations, which is possible evidence of zeaxanthin-chlorophyll charge-transfer (CT) quenching, was investigated in spinach thylakoids...
November 16, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/29052196/3c-in-maize-and-arabidopsis
#16
Blaise Weber, Suraj Jamge, Maike Stam
With Chromosome Conformation Capture (3C), the relative interaction frequency of one chromosomal fragment with another can be determined. The technique is especially suited for unraveling the 3D organization of specific loci when focusing on aspects such as enhancer-promoter interactions or other topological conformations of the genome. 3C has been extensively used in animal systems, among others providing insight into gene regulation by distant cis-regulatory elements. In recent years, the 3C technique has been applied in plant research...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28973714/a-rapd-pcr-based-genetic-diversity-analysis-of-helicoverpa-armigera-and-h-zea-populations-in-brazil
#17
H M Lopes, C S Bastos, L S Boiteux, J Foresti, F A Suinaga
Helicoverpa armigera is the most significant pest of agriculture in Asia, Europe, Africa, and Australasia, causing damage to crops greater than US$2 billion annually and until 2013 it was not detected in Brazil. Helicoverpa zea is restricted to the American continent and is important to corn and a secondary pest of cotton and tomatoes. The wide range of crops exploited by H. armigera (mainly cotton, soybeans, chickpea, and corn), the possible mating between these species can promote population shifts, that could be assessed by RAPD-PCR technique...
September 21, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28973573/further-description-of-helicoverpa-zea-lepidoptera-noctuidae-male-genitalia-and-new-genetic-evidence-of-synonymy-with-respect-to-the-anomalous-form-heliothis-stombleri
#18
Emilia I Balbi, Fernando M Flores, Daniela S Tosto, Joel D Arneodo
The Helicoverpa/Heliothis complex can cause serious damage to agricultural crops. Phenotypic similarity makes it difficult to discriminate between closely related Helicoverpa species. Currently, morphology of the male genitalia complemented with molecular techniques constitutes the best approach for species identification. In this work, a broad microscopic examination of adult Helicoverpa zea (Boddie) males (n = 200) captured in central Argentina was carried out in order to provide a detailed description of the valvae and the phallus...
May 1, 2017: Journal of Insect Science
https://www.readbyqxmd.com/read/28969654/viral-metagenomics-of-aphids-present-in-bean-and-maize-plots-on-mixed-use-farms-in-kenya-reveals-the-presence-of-three-dicistroviruses-including-a-novel-big-sioux-river-virus-like-dicistrovirus
#19
Francis O Wamonje, George N Michuki, Luke A Braidwood, Joyce N Njuguna, J Musembi Mutuku, Appolinaire Djikeng, Jagger J W Harvey, John P Carr
BACKGROUND: Aphids are major vectors of plant viruses. Common bean (Phaseolus vulgaris L.) and maize (Zea mays L.) are important crops that are vulnerable to aphid herbivory and aphid-transmitted viruses. In East and Central Africa, common bean is frequently intercropped by smallholder farmers to provide fixed nitrogen for cultivation of starch crops such as maize. We used a PCR-based technique to identify aphids prevalent in smallholder bean farms and next generation sequencing shotgun metagenomics to examine the diversity of viruses present in aphids and in maize leaf samples...
October 2, 2017: Virology Journal
https://www.readbyqxmd.com/read/28900437/genetics-of-resistance-and-pathogenicity-in-the-maize-setosphaeria-turcica-pathosystem-and-implications-for-breeding
#20
REVIEW
Ana L Galiano-Carneiro, Thomas Miedaner
Northern corn leaf blight (NCLB), the most devastating leaf pathogen in maize (Zea mays L.), is caused by the heterothallic ascomycete Setosphaeria turcica. The pathogen population shows an extremely high genetic diversity in tropical and subtropical regions. Varietal resistance is the most efficient technique to control NCLB. Host resistance can be qualitative based on race-specific Ht genes or quantitative controlled by many genes with small effects. Quantitative resistance is moderately to highly effective and should be more durable combatting all races of the pathogen...
2017: Frontiers in Plant Science
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