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https://www.readbyqxmd.com/read/28549716/automated-programmable-pressurized-carbonic-acid-eluent-ion-exclusion-chromatography-of-organic-acids
#1
C Phillip Shelor, Purnendu K Dasgupta
Conductometric ion exclusion chromatography of weak acids does not enjoy the same degree of success as its counterpart in suppressed conductometric anion exchange chromatography. Compared to the latter, eluent preparation is not automated. Commonly, a strong acid is used as the eluent leading to a relatively high-detector background and poorer limits of detection. In addition, generally applicable gradient elution has not been possible. We introduce the automated preparation of carbonic acid eluents at concentrations exceeding 0...
May 16, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28545109/hyperbaric-oxygen-therapy-for-wound-healing-in-diabetic-rats-varying-efficacy-after-a-clinically-based-protocol
#2
Johan W van Neck, Bastiaan Tuk, Esther M G Fijneman, Jonathan J Redeker, Edwin M Talahatu, Miao Tong
Hyperbaric oxygen therapy (HBOT) is a clinical treatment in which a patient breathes pure oxygen for a limited period of time at an increased pressure. Although this therapy has been used for decades to assist wound healing, its efficacy for many conditions is unproven and its mechanism of action is not yet fully clarified. This study investigated the effects of HBOT on wound healing using a diabetes-impaired pressure ulcer rat model. Seven weeks after streptozotocin-induced diabetes in rats (n = 55), a pressure ulcer was created on dorsal skin...
2017: PloS One
https://www.readbyqxmd.com/read/28544641/reflectance-spectroscopy-to-shed-new-light-on-the-capillary-refill-test
#3
Rani Toll John, Joakim Henricson, Gert E Nilsson, Daniel Wilhelms, Chris D Anderson
To use Bioengineering methodology is used to achieve, at five anatomical sites, a detailed, quantitative assessment of the return of blood content to the blanched area, during the Capillary Refill (CR) test. An observational, non-randomized, experimental study on 23 healthy subjects (14 females) was performed in our climate controlled skin physiology laboratory. Our main outcome measures were based on the chronological assessment and quantification of red blood cell concentration (RBC) after the release of blanching pressure in the CR test, using Tissue Viability Imaging (TiVi), a digital photographic technique based on polarisation spectroscopy...
May 24, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28544429/diabetes-and-cardiometabolic-risk-factors-in-cambodia-results-from-two-screening-studies
#4
Julie Wagner, Diana Naranjo, Touch Khun, Serey Seng, Ien Srey Horn, Sakinah Carter Suttiratana, Lim Keuky
BACKGROUND: Despite growing attention to diabetes throughout Asia, data from Southeast Asia are limited. This article reports rates of diabetes, hypertension and obesity in Cambodia. METHODS: Two studies were conducted across different regions of Cambodia: a 2012 screening study across urban, semi-urban and rural areas that used point of care capillary glucose for determination of diabetes (n = 13,997) and a 2005 epidemiological study with random selection from two main urban areas that used oral glucose tolerance tests for determination of diabetes (n = 1,863)...
May 22, 2017: Journal of Diabetes
https://www.readbyqxmd.com/read/28542612/interface-condition-for-the-darcy-velocity-at-the-water-oil-flood-front-in-the-porous-medium
#5
Xiaolong Peng, Yong Liu, Baosheng Liang, Zhimin Du
Flood front is the jump interface where fluids distribute discontinuously, whose interface condition is the theoretical basis of a mathematical model of the multiphase flow in porous medium. The conventional interface condition at the jump interface is expressed as the continuous Darcy velocity and fluid pressure (named CVCM). Our study has inspected this conclusion. First, it is revealed that the principle of mass conservation has no direct relation to the velocity conservation, and the former is not the true foundation of the later, because the former only reflects the kinetic characteristic of the fluid particles at one position(the interface), but not the different two parts of fluid on the different side of the interface which required by the interface conditions...
2017: PloS One
https://www.readbyqxmd.com/read/28539568/clinical-and-hemodynamic-effects-of-endothelin-receptor-antagonists-in-patients-with-heart-failure
#6
Bo Xiong, Dan Nie, Yin Cao, Yanke Zou, Yuanqing Yao, Jie Tan, Jun Qian, Shunkang Rong, Chunbin Wang, Jing Huang
The clinical benefit of endothelin receptor antagonists (ERA) for the management of heart failure (HF) remains controversial. To examine this question, we performed a meta-analysis of randomized controlled trials (RCTs) to investigate the clinical and hemodynamic effects of ERA in HF patients.We searched the PubMed, Medline, Embase, and Cochrane Library from inception to March 20, 2016 to identify the pertinent studies. Risk ratio (RR) and weighted mean difference (WMD) were calculated using a fixed or random effect model...
May 23, 2017: International Heart Journal
https://www.readbyqxmd.com/read/28533908/ascites-refractory-ascites-and-hyponatremia-in-cirrhosis
#7
Brett Fortune, Andres Cardenas
Ascites is the most common complication related to cirrhosis and is associated with increased morbidity and mortality. Ascites is a consequence of the loss of compensatory mechanisms to maintain the overall effective arterial blood volume due to worsening splanchnic arterial vasodilation as a result of clinically significant portal hypertension. In order to maintain effective arterial blood volume, vasoconstrictor and antinatriuretic pathways are activated, which increase overall sodium and fluid retention...
May 2017: Gastroenterology Report
https://www.readbyqxmd.com/read/28531295/right-ventricular-vascular-coupling-in-heart-failure-with-preserved-ejection-fraction-and-pre-vs-post-capillary-pulmonary-hypertension
#8
Thomas M Gorter, Dirk J van Veldhuisen, Adriaan A Voors, Yoran M Hummel, Carolyn S P Lam, Rolf M F Berger, Joost P van Melle, Elke S Hoendermis
Aims: Many patients with heart failure with preserved ejection fraction (HFpEF) develop post-capillary pulmonary hypertension (PH) due to increased left-sided filling pressures. However, a subset of patients develops combined post- and pre-capillary PH. We studied the value of echocardiographic right-sided characterization for the discrimination between pre- vs. post-capillary PH in HFpEF, using invasive haemodynamics as gold standard. Methods and results: 102 consecutive HFpEF patients with simultaneous right heart catheterization and echocardiography were identified...
May 22, 2017: European Heart Journal Cardiovascular Imaging
https://www.readbyqxmd.com/read/28528550/isotachophoretic-fluorescence-in-situ-hybridization-of-intact-bacterial-cells
#9
Sui Ching Phung, Joan Marc Cabot, Mirek Macka, Shane M Powell, Rosanne M Guijt, Michael C Breadmore
A counter-pressure assisted capillary isotachophoresis method in combination with a sieving matrix and ionic spacer was used to perform in-line fluorescence in situ hybridization (FISH) of bacterial cells. A high concentration of sieving matrix (1.8% w/v HEC) was introduced at one end of the capillary and the bacterial cells were suspended in the spacer electrolyte for injection. Using a 2 min injection with 18 psi counter-pressure, 50% of the cells injected into the capillary were hybridized with the fluorescently labelled oligonucleotide and the excess unhybridized probe was separated from the hybridized cells-probe complexes in a two stage ITP method...
May 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28527962/resolution-of-mitral-regurgitation-with-left-ventricular-assist-device-support
#10
Matthew Goodwin, Hassan W Nemeh, Jamil Borgi, Gaetano Paone, Jeffrey A Morgan
BACKGROUND: Cardiac valve disease is common in patients with end-stage heart failure undergoing left ventricular assist device implantation (LVAD). The aim of this study was to determine if preoperative mitral regurgitation (MR) affects outcomes and hemodynamics in patients after LVAD implantation. METHODS: From March 2006 through May 2015, 238 consecutive patients underwent LVAD implantation. Patient cohorts included less than moderate to severe MR (< moderate-severe, n = 195) and greater than or equal to moderate to severe MR (≥ moderate-severe, n = 43)...
May 17, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28523563/assessment-of-endothelial-cell-function-and-physiological-microcirculatory-reserve-by-video-microscopy-using-a-topical-acetylcholine-and-nitroglycerin-challenge
#11
Matthias Peter Hilty, Jacqueline Pichler, Bulent Ergin, Urs Hefti, Tobias Michael Merz, Can Ince, Marco Maggiorini
BACKGROUND: Assessment of the microcirculation is a promising target for the hemodynamic management of critically ill patients. However, just as the sole reliance on macrocirculatory parameters, single static parameters of the microcirculation may not represent a sufficient guide. Our hypothesis was that by serial topical application of acetylcholine (ACH) and nitroglycerin (NG), the sublingual microcirculation can be challenged to determine its endothelial cell-dependent and smooth muscle-dependent physiological reserve capacity...
December 2017: Intensive Care Medicine Experimental
https://www.readbyqxmd.com/read/28523153/pleural-pressure-theory-revisited-a-role-for-capillary-equilibrium
#12
Aaron R Casha, Roberto Caruana-Gauci, Alexander Manche, Marilyn Gauci, Stanley Chetcuti, Luca Bertolaccini, Marco Scarci
BACKGROUND: Theories elucidating pleural pressures should explain all observations including the equal and opposite recoil of the chest wall and lungs, the less than expected pleural hydrostatic gradient and its variation at lobar margins, why pleural pressures are negative and how pleural fluid circulation functions. METHODS: A theoretical model describing equilibrium between buoyancy, hydrostatic forces, and capillary forces is proposed. The capillary equilibrium model described depends on control of pleural fluid volume and protein content, powered by an active pleural pump...
April 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28518116/the-wincf-model-an-inexpensive-and-tractable-microcosm-of-a-mucus-plugged-bronchiole-to-study-the-microbiology-of-lung-infections
#13
William J Comstock, Edwin Huh, Reiley Weekes, Connor Watson, Tianyang Xu, Pieter C Dorrestein, Robert A Quinn
Many chronic airway diseases result in mucus plugging of the airways. Lungs of an individual with cystic fibrosis are an exemplary case where their mucus-plugged bronchioles create a favorable habitat for microbial colonization. Various pathogens thrive in this environment interacting with each other and driving many of the symptoms associated with CF disease. Like any microbial community, the chemical conditions of their habitat have a significant impact on the community structure and dynamics. For example, different microorganisms thrive in differing levels of oxygen or other solute concentrations...
May 8, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28515936/pulse-dose-steroids-in-severe-pulmonary-arterial-hypertension-secondary-to-systemic-lupus-erythematosus
#14
Cody Lee, Jean Elwing
OBJECTIVE: The pulmonary vascular targeted treatment for systemic lupus erythematosus-associated pulmonary arterial hypertension is similar to other connective tissue disease-associated pulmonary arterial hypertension. In addition, there also appears to be a role for immunosuppression in the overall management. However, the optimal immunosuppressive regimen and what patients will respond to treatments are currently not clearly elucidated given the lack of randomized controlled trials on the subject...
2017: SAGE open medical case reports
https://www.readbyqxmd.com/read/28515821/left-ventricular-velocity-of-propagation-a-useful-non-invasive-measurement-when-assessing-hemodynamic-alterations-in-pulmonary-arterial-hypertension
#15
Dagmar F Hernandez-Suarez, Denada Palm, Francisco Lopez-Menendez, Marcel Mesa Pabon, Angel Lopez-Candales
BACKGROUND: Left ventricular (LV) velocity of propagation (Vp) has been shown to be inversely related to the LV relaxation time constant. We sought to examine Vp from a group of chronic pulmonary hypertension (cPH) patients and compare these values to Vp obtained in normal individuals and patients with known LV diastolic dysfunction (LVDD). METHODS: Echo-Doppler data and Vp measurements were retrospectively collected from all patients. The studied population was divided into four groups...
April 2017: Cardiology Research
https://www.readbyqxmd.com/read/28512313/a-rapid-capillary-pressure-driven-micro-channel-to-demonstrate-newtonian-fluid-behavior-of-zebrafish-blood-at-high-shear-rates
#16
Juhyun Lee, Tzu-Chieh Chou, Dongyang Kang, Hanul Kang, Junjie Chen, Kyung In Baek, Wei Wang, Yichen Ding, Dino Di Carlo, Yu-Chong Tai, Tzung K Hsiai
Blood viscosity provides the rheological basis to elucidate shear stress underlying developmental cardiac mechanics and physiology. Zebrafish is a high throughput model for developmental biology, forward-genetics, and drug discovery. The micro-scale posed an experimental challenge to measure blood viscosity. To address this challenge, a microfluidic viscometer driven by surface tension was developed to reduce the sample volume required (3μL) for rapid (<2 min) and continuous viscosity measurement. By fitting the power-law fluid model to the travel distance of blood through the micro-channel as a function of time and channel configuration, the experimentally acquired blood viscosity was compared with a vacuum-driven capillary viscometer at high shear rates (>500 s(-1)), at which the power law exponent (n) of zebrafish blood was nearly 1 behaving as a Newtonian fluid...
May 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28511930/bed-morphological-features-associated-with-an-optimal-slurry-concentration-for-reproducible-preparation-of-efficient-capillary-ultrahigh-pressure-liquid-chromatography-columns
#17
Arved E Reising, Justin M Godinho, James W Jorgenson, Ulrich Tallarek
Column wall effects and the formation of larger voids in the bed during column packing are factors limiting the achievement of highly efficient columns. Systematic variation of packing conditions, combined with three-dimensional bed reconstruction and detailed morphological analysis of column beds, provide valuable insights into the packing process. Here, we study a set of sixteen 75μm i.d. fused-silica capillary columns packed with 1.9μm, C18-modified, bridged-ethyl hybrid silica particles slurried in acetone to concentrations ranging from 5 to 200mg/mL...
May 4, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28506612/identification-of-pulmonary-hypertension-caused-by-left-heart-disease-world-health-organization-group-2-based-on-cardiac-chamber-volumes-derived-from-chest-ct
#18
Galit Aviram, Zach Rozenbaum, Tomer Ziv-Baran, Shlomo Berliner, Yan Topilsky, Dominik Fleischmann, Yon K Sung, Roham T Zamanian, Haiwei Henry Guo
BACKGROUND: Evaluations of patients with pulmonary hypertension (PH) commonly include chest computed tomography (CT). We hypothesized that cardiac chamber volumes calculated from the same CT scans can yield additional information to distinguish left heart disease-related PH (WHO Group 2) from other PH subtypes. METHODS: Patients with right heart catheterization (RHC)-confirmed PH and contrast-enhanced chest CT studies were enrolled in this retrospective multicenter study...
May 12, 2017: Chest
https://www.readbyqxmd.com/read/28505748/highly-pressurized-partially-miscible-liquid-liquid-flow-in-a-micro-t-junction-i-experimental-observations
#19
Ning Qin, John Z Wen, Carolyn L Ren
This is the first part of a two-part study on a partially miscible liquid-liquid flow (liquid carbon dioxide and deionized water) which is highly pressurized and confined in a microfluidic T-junction. Our main focuses are to understand the flow regimes as a result of varying flow conditions and investigate the characteristics of drop flow distinct from coflow, with a capillary number, Ca_{c}, that is calculated based on the continuous liquid, ranging from 10^{-3} to 10^{-2} (10^{-4} for coflow). Here in part I, we present our experimental observation of drop formation cycle by tracking drop length, spacing, frequency, and after-generation speed using high-speed video and image analysis...
April 2017: Physical Review. E
https://www.readbyqxmd.com/read/28500695/clinical-characteristics-and-survival-of-systemic-sclerosis-patients-with-pulmonary-hypertension-and-elevated-wedge-pressure-observations-from-the-pharos-cohort
#20
Matthew R Lammi, Lesley A Saketkoo, Jessica K Gordon, Paula Lauto, Karen Fagan, Virginia D Steen
BACKGROUND AND OBJECTIVE: Systemic sclerosis (SSc) is a complex autoimmune disease commonly associated with pulmonary hypertension (PH). When associated with elevated pulmonary artery wedge pressure (PAWP), pulmonary artery pressure (PAP) is either in-proportion (post-capillary PH) or higher than expected (combined PH) relative to the increased PAWP. METHODS: Patients from the PHAROS registry (a prospective observational cohort of SSc-PH patients) who had mean PAP ≥ 25 and PAWP > 15 on right heart catheterization were stratified based on diastolic pressure gradient (DPG)...
May 12, 2017: Respirology: Official Journal of the Asian Pacific Society of Respirology
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