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https://www.readbyqxmd.com/read/29453254/development-of-a-versatile-cas9-driven-subpopulation-selection-toolbox-in-lactococcus-lactis
#1
Simon van der Els, Jennelle K James, Michiel Kleerebezem, Peter A Bron
CRISPR-Cas9 technology has been exploited for the removal or replacement of genetic elements in a wide range of prokaryotes and eukaryotes. Here we describe the extension of the Cas9 application toolbox to the industrially important dairy species Lactococcus lactis The Cas9 expression vector pLABTarget was constructed, encoding the Streptocccus pyogenes Cas9 under control of a constitutive promoter, and allowing plug and play introduction of sgRNA sequences to target specific genetic loci. Introduction of a pepN -targeting derivative of pLABTarget into L...
February 16, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29453074/use-of-prostaglandin-f2%C3%AE-as-ovulatory-stimulus-for-synchronizing-dairy-cattle
#2
N A Castro, P M A Neves, J P Cestaro, V T O Melo, A Schneider, L F M Pfeifer
The aim of this study was to evaluate if prostaglandin F2α (PGF) can be used to induce ovulation in a GnRH-progesterone based protocol. In Experiment 1 crossbred dairy cows (n=32) were synchronized with a progesterone-GnRH based protocol for seven days, where the luteolytic dose of 150μg PGF was given 24h prior progesterone device removal (CIDR). On Day 8 cows were separated into two groups to receive: 1) 2mL of Saline (Control Group, n=15) or 2) 150μg of PGF (PGF Group, n=17). Ovulation rate was higher in the PGF than Control group (100% vs 53...
January 31, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29451639/-analysis-of-foods-advertised-to-childhood-audience-on-chilean-television
#3
Catalina González-Hidalgo
OBJECTIVE: To examine food advertising aimed at childhood's audience broadcast on Chilean television. MATERIALS AND METHODS: Cross-sectional study. Food advertising broadcast by 4 open signal TV channels was recorded for 12 hours daily on twelve days randomly selected. Notices were classified according to food groups and level of processing. The frequency of food advertisement aimed to childhood audience was compared with those for adults. Nutrient profile of foods advertised was described...
November 2017: Salud Pública de México
https://www.readbyqxmd.com/read/29451594/nontuberculous-mycobacteria-in-milk-from-positive-cows-in-the-intradermal-comparative-cervical-tuberculin-test-implications-for-human-tuberculosis-infections
#4
Carmen Alicia Daza Bolaños, Marília Masello Junqueira Franco, Antonio Francisco Souza Filho, Cássia Yumi Ikuta, Edith Mariela Burbano-Rosero, José Soares Ferreira Neto, Marcos Bryan Heinemann, Rodrigo Garcia Motta, Carolina Lechinski de Paula, Amanda Bonalume Cordeiro de Morais, Simony Trevizan Guerra, Ana Carolina Alves, Fernando José Paganini Listoni, Márcio Garcia Ribeiro
Although the tuberculin test represents the main in vivo diagnostic method used in the control and eradication of bovine tuberculosis, few studies have focused on the identification of mycobacteria in the milk from cows positive to the tuberculin test. The aim of this study was to identify Mycobacterium species in milk samples from cows positive to the comparative intradermal test. Milk samples from 142 cows positive to the comparative intradermal test carried out in 4,766 animals were aseptically collected, cultivated on Lowenstein-Jensen and Stonebrink media and incubated for up to 90 days...
2018: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/29450980/prediction-of-enteric-methane-production-yield-and-intensity-in-dairy-cattle-using-an-intercontinental-database
#5
Mutian Niu, Ermias Kebreab, Alexander N Hristov, Joonpyo Oh, Claudia Arndt, André Bannink, Ali R Bayat, André F Brito, Tommy Boland, David Casper, Les A Crompton, Jan Dijkstra, Maguy A Eugène, Phil C Garnsworthy, Md Najmul Haque, Anne L F Hellwing, Pekka Huhtanen, Michael Kreuzer, Bjoern Kuhla, Peter Lund, Jørgen Madsen, Cécile Martin, Shelby C McClelland, Mark McGee, Peter J Moate, Stefan Muetzel, Camila Muñoz, Padraig O'Kiely, Nico Peiren, Christopher K Reynolds, Angela Schwarm, Kevin J Shingfield, Tonje M Storlien, Martin R Weisbjerg, David R Yáñez-Ruiz, Zhongtang Yu
Enteric methane (CH 4 ) production from cattle contributes to global greenhouse gas emissions. Measurement of enteric CH 4 is complex, expensive and impractical at large scales; therefore, models are commonly used to predict CH 4 production. However, building robust prediction models requires extensive data from animals under different management systems worldwide. The objectives of this study were to (1) collate a global database of enteric CH 4 production from individual lactating dairy cattle; (2) determine the availability of key variables for predicting enteric CH 4 production (g/d per cow), yield [g/kg dry matter intake (DMI)], and intensity (g/kg energy corrected milk) and their respective relationships; (3) develop intercontinental and regional models and cross-validate their performance; and (4) assess the trade-off between availability of on-farm inputs and CH 4 prediction accuracy...
February 16, 2018: Global Change Biology
https://www.readbyqxmd.com/read/29449405/draft-genome-sequence-of-lactobacillus-helveticus-atcc-12046
#6
María Mercedes Palomino, Germán F Burguener, Josefina Campos, Mariana Allievi, Joaquina Fina-Martin, Mariano Prado Acosta, Darío A Fernández Do Porto, Sandra M Ruzal
Lactobacillus helveticus is a lactic acid bacterium used traditionally in the dairy industry, especially in the manufacture of cheeses. We present here the 2,141,841-bp draft genome sequence of L. helveticus strain ATCC 12046, a potential starter strain for improving cheese production.
February 15, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29448889/potential-of-the-moringa-oleifera-saline-extract-for-the-treatment-of-dairy-wastewater-application-of-the-response-surface-methodology
#7
Dalila Maria Formentini-Schmitt, Márcia Regina Fagundes-Klen, Márcia Teresinha Veit, Soraya Moreno Palácio, Daniela Estelita Goes Trigueros, Rosangela Bergamasco, Gustavo Affonso Pisano Mateus
In this work, the coagulation/flocculation/sedimentation treatment of dairy wastewater samples was investigated through serial factorial designs utilizing the saline extract obtained from Moringa oleifera (Moringa) as a coagulant. The sedimentation time, pH, Moringa coagulant (MC) dose and concentration of CaCl 2 have been evaluated through the response surface methodology (RSM) in order to obtain the ideal turbidity removal conditions. The empirical quadratic model, in conjunction with the desirability function, demonstrated that it is possible to obtain turbidity removals of 98...
February 16, 2018: Environmental Technology
https://www.readbyqxmd.com/read/29448169/molecular-spectroscopic-features-of-protein-in-newly-developed-chickpea-relationship-with-protein-chemical-profile-and-metabolism-in-the-rumen-and-intestine-of-dairy-cows
#8
Baoli Sun, Nazir Ahmad Khan, Peiqiang Yu
The first aim of this study was to investigate the nutritional value of crude protein (CP) in CDC [Crop Development Centre (CDC), University of Saskatchewan] chickpea varieties (Frontier kabuli and Corinne desi) in comparison with a CDC barley variety in terms of: 1) CP chemical profile and subfractions; (2) in situ rumen degradation kinetics and intestinal digestibility of CP; 2) metabolizable protein (MP) supply to dairy cows; and (3) protein molecular structure characteristics using advanced molecular spectroscopy...
February 6, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29448094/the-prevalence-and-antimicrobial-resistance-of-listeria-spp-in-raw-milk-and-traditional-dairy-products-delivered-in-yazd-central-iran-2016
#9
Fateme Akrami-Mohajeri, Zahra Derakhshan, Margherita Ferrantee, Negar Hamidiyan, Meysam Soleymani, Gea Oliveri Conti
Listeria monocytogenes is an important foodborne pathogen which its data in Iranian dairy products is limited. A total of 545 samples of traditional dairy products (raw milk, traditional cheese, traditional butter, traditional curd, and traditional ice cream) were collected from traditional dairy shops located in Yazd, Iran. L. monocytogenes was isolated, and positive samples were confirmed by polymerase chain reaction. Antibiotic sensitivity test was conducted to determine the antibiotic resistance. Listeria species were isolated from 11...
February 12, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29448092/investigation-on-bisphenol-a-levels-in-human-milk-and-dairy-supply-chain-a-review
#10
REVIEW
Raffaelina Mercogliano, Serena Santonicola
Bisphenol A (BPA), widely used as additive in food packaging, is an environmental and food contaminant that shows a weak estrogenic activity in general population and toxicity in the infant population. A temporary tolerable daily intake (t-TDI) of 4 μg/kg bw/day and a migration limit of 0.6 mg/kg in food from plastic materials, intended to come in contact with food, were fixed. Dietary milk is important in the human diet. The review investigated the contamination levels in human milk and along the dairy supply chain...
February 12, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29447209/haplotype-based-genome-wide-association-study-identifies-loci-and-candidate-genes-for-milk-yield-in-holsteins
#11
Zhenliang Chen, Yunqiu Yao, Peipei Ma, Qishan Wang, Yuchun Pan
Since milk yield is a highly important economic trait in dairy cattle, the genome-wide association study (GWAS) is vital to explain the genetic architecture underlying milk yield and to perform marker-assisted selection (MAS). In this study, we adopted a haplotype-based empirical Bayesian GWAS to identify the loci and candidate genes for milk yield. A total of 1 092 Holstein cows were sequenced by using the genotyping by genome reducing and sequencing (GGRS) method. After filtering, 164 312 high-confidence SNPs and 13 476 haplotype blocks were identified to use for GWAS...
2018: PloS One
https://www.readbyqxmd.com/read/29446933/dietary-sodium-butyrate-supplementation-reduces-high-concentrate-diet-feeding-induced-apoptosis-in-mammary-cells-in-dairy-goats
#12
Guangjun Chang, Jinyu Yan, Nana Ma, Xinxin Liu, Hongyu Dai, Muhammad Shaid Bilal, Xiangzhen Shen
Eighteen lactating goats (38.86 ± 2.06 kg) were randomly allocated to three groups. One group was fed a low-concentrate (LC) diet (forage:concentrate = 6:4, LC), while the other two groups were fed a high-concentrate (HC) diet (forage:concentrate = 4:6, HC) or an HC diet supplemented with sodium butyrate (BHC) for 20 weeks. Samples of ruminal fluid, milk, hepatic blood plasma, and mammary gland tissue were prepared for the experimental analysis. The LPS concentration, caspase-3 and -8 enzymatic activity, caspase-3 and -8 mRNA expression, and NF-κB (p65), phosphorylated-p65, bax, cytochrome C, and caspase-3 protein expression were higher in the HC group than those in the LC group; however, the levels of these parameters were lower in the BHC group than those in the HC group...
February 15, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29445929/prevalence-of-leptospira-serogroup-specific-antibodies-in-cattle-associated-with-reproductive-problems-in-endemic-states-of-india
#13
Vinayagamurthy Balamurugan, Anusha Alamuri, K Bharathkumar, Sharanagouda Siddanagouda Patil, Gurrappa Naidu Govindaraj, Mohandoss Nagalingam, Paramanandham Krishnamoorthy, Habibur Rahman, Bibek Ranjan Shome
In this study, the seroprevalence and distribution of Leptospira in dairy cattle in endemic states of India were investigated in association with reproductive problems of the cattle. A total of 373 cattle serum samples from 45 farms in Maharashtra, Gujarat, Andhra Pradesh, Telangana, Karnataka, Tamil Nadu, Punjab, Haryana, Chhattisgarh, Sikkim and Uttarakhand states were collected from animals with a history of reproductive disorders like abortion, repeat breeding, anoestrus and endometritis, and also from apparently healthy animals...
February 14, 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29445861/bovine-neutrophils-in-health-and-disease
#14
REVIEW
Laura L Bassel, Jeff L Caswell
Bovine neutrophils have similarities to those of other species with respect to mechanisms of their activation and migration into tissue, modulation of immune responses and the balance between microbial killing and host tissue damage. However, bovine neutrophils have biochemical and functional differences from those of other species, which may yield insights about the comparative biology of neutrophils. Neutrophils play protective and harmful roles in the infectious diseases of cattle that occur at times of transition: respiratory disease in beef calves recently arrived to feedlots and mastitis and other diseases of postparturient dairy cows...
February 14, 2018: Cell and Tissue Research
https://www.readbyqxmd.com/read/29445454/rumen-protected-methionine-during-the-peripartal-period-in-dairy-cows-and-its-effects-on-abundance-of-major-species-of-ruminal-bacteria
#15
Mohamed K Abdelmegeid, Ahmed A Elolimy, Zheng Zhou, Vincenzo Lopreiato, Joshua C McCann, Juan J Loor
Background: Extensive degradation of amino acids in the rumen via microbial deamination decreases the post-ruminal availability of dietary indispensable amino acids. Together with the normal decrease in voluntary dry matter intake (DMI) around parturition in dairy cows, microbial metabolism contributes to a markedly negative balance of indispensable amino acids, including methionine which may be the first-limiting for milk production. The main objective of the current study was to profile changes in major bacterial species with key functions in cellulose and hemicellulose digestion, xylan breakdown, proteolytic action, propionic acid production, lactate utilization and ruminal biohydrogenation in cows supplemented with rumen-protected Methionine (SM; Smartamine M, Adisseo NA, Alpharetta, GA, USA) from -23 through 30 d relative to parturition...
2018: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/29445366/the-effect-of-dna-extraction-methods-on-observed-microbial-communities-from-fibrous-and-liquid-rumen-fractions-of-dairy-cows
#16
Jueeli D Vaidya, Bartholomeus van den Bogert, Joan E Edwards, Jos Boekhorst, Sanne van Gastelen, Edoardo Saccenti, Caroline M Plugge, Hauke Smidt
DNA based methods have been widely used to study the complexity of the rumen microbiota, and it is well known that the method of DNA extraction is a critical step in enabling accurate assessment of this complexity. Rumen fluid (RF) and fibrous content (FC) fractions differ substantially in terms of their physical nature and associated microorganisms. The aim of this study was therefore to assess the effect of four DNA extraction methods (RBB, PBB, FDSS, PQIAmini) differing in cell lysis and/or DNA recovery methods on the observed microbial diversity in RF and FC fractions using samples from four rumen cannulated dairy cows fed 100% grass silage (GS100), 67% GS and 33% maize silage (GS67MS33), 33% GS and 67% MS (GS33MS67), or 100% MS (MS100)...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29444092/the-impact-of-early-life-nutrition-and-housing-on-growth-and-reproduction-in-dairy-cattle
#17
G Curtis, C McGregor Argo, D Jones, D Grove-White
Contentious issues in calf rearing include milk feeding practices and single versus group housing. The current study was performed on a high producing 170 Holstein cow dairy farm, to investigate the impact of nutrition and housing on growth and reproduction. Heifer calves (n = 100) were allocated in birth order to one of two commonly used management strategies. All calves received 3-4 litres of dam specific colostrum within 6 hours of birth. Group A calves were group housed from birth and fed milk replacer (MR) ad libitum via a computerised machine utilising a single teat, with weaning commencing at 63 days of age...
2018: PloS One
https://www.readbyqxmd.com/read/29442457/dietary-patterns-and-breast-or-lung-cancer-risk-a-pooled-analysis-of-2-case-control-studies-in-north-eastern-poland
#18
Beata Krusińska, Iwona Hawrysz, Małgorzata A Słowińska, Lidia Wądołowska, Maciej Biernacki, Anna Czerwińska, Janusz J Gołota
BACKGROUND: Breast cancer in women and lung cancer in men are the most prevalent cancers in Poland and worldwide. Evidence of the impact of food groups and nutrients on the risk of breast and lung cancer is limited and lacking conclusions. Studies on food consumption and breast or lung cancer are limited. OBJECTIVES: Assessment of the association between dietary patterns and the prevalence of breast and lung cancers in adult Poles. MATERIAL AND METHODS: The study involved a pooled analysis of 2 case-control studies on 320 subjects aged 50-70 years from north-eastern Poland (160 women, 160 men)...
December 2017: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/29442254/heat-stress-and-effect-of-shade-materials-on-hormonal-and-behavior-response-of-dairy-cattle-a-review
#19
REVIEW
Reena Kamal, Triveni Dutt, Manjunath Patel, Amitava Dey, Panch Kishore Bharti, Poolangulam Chinnakkan Chandran
In tropical countries, at high temperatures, several physiological rearrangements occur in cows as they attempt to facilitate heat dissipation and/or reduce metabolic heat production. Following exposure to heat, cattle appear to acclimatize within 2-7 weeks. The failure of homeostasis at high temperatures may lead to reduced productivity or even death. The situation is even worse when humidity adds to high temperature. Livestock with elevated body temperature exhibit lower DMI and growth with less efficiency, reducing profitability for dairy farms in hot and humid climates...
February 13, 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29441408/macronutrient-composition-and-food-groups-associated-with-gestational-weight-gain-the-gusto-study
#20
Jun S Lai, Shu E Soh, See Ling Loy, Marjorelee Colega, Michael S Kramer, Jerry K Y Chan, Thiam Chye Tan, Lynnette P C Shek, Fabian K P Yap, Kok Hian Tan, Keith M Godfrey, Yap Seng Chong, Mary F F Chong
PURPOSE: To examine the associations of energy, macronutrient and food intakes with GWG on 960 pregnant women from the Growing Up in Singapore Towards healthy Outcomes (GUSTO) mother-offspring cohort. METHODS: Dietary intake was assessed at 26-28 weeks' gestation with a 24-hour recall and 3-day food diary. GWG z-scores were calculated from first (4-13 weeks' gestation) and last (30-40 weeks gestation) measured weights; inadequate and excessive GWG were defined using the Institute of Medicine recommendations based on weights between 15 and 35 weeks' gestation...
February 13, 2018: European Journal of Nutrition
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