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Massi melanoma

Gongda Xue, Emanuela Romano, Daniela Massi, Mario Mandalà
WNT signaling regulates embryonic development and tissue homeostasis in the adult stage. Evolutionarily, activation of the WNT pathway is triggered by a large family of cytokines and activates a broad spectrum of downstream targets through two independent branches mediated by β-catenin (defined as canonical pathway) or PLC and small GTPase (defined as non-canonical pathway), respectively. Recent studies revealed the crucial role of WNT in the maintenance of cell metabolism and stemness as well as its deregulation in tumourigenesis and malignant transformation through oncogenic reprogramming, which contributes to cancer cell proliferation and differentiation, survival, stress response and resistance...
September 2016: Cancer Treatment Reviews
Anna Falanga, Marina Marchetti, Daniela Massi, Barbara Merelli, Cristina Verzeroli, Laura Russo, Eliana Rulli, Carlo Tondini, Lorenzo Legramandi, Romina Nassini, Cristian Scatena, Francesco De Logu, Laura Cattaneo, Mario Mandalà
No abstract text is available yet for this article.
June 2016: Journal of the American Academy of Dermatology
Sara Gandini, Daniela Massi, Mario Mandalà
BACKGROUND: Despite the success of immunotherapy directed at inhibiting of programmed death-1 (PD-1)/PD-ligand (L)1 signaling, it is not established whether PD-L1 expression correlates with the clinical response and outcome in different tumors. The present meta-analysis investigates whether the PD-L1 status, detected by immunohistochemistry, is associated with clinical response and mortality in patients treated with anti-PD-1/PD-L1 therapy. METHODS: A systematic literature search and quantitative analysis were planned, conducted and reported following CONSORT and QUORUM checklists, up to December 2015, to identify clinical trials with information on cancer outcome by PD-L1 immunohistochemical expression in tumor tissues...
April 2016: Critical Reviews in Oncology/hematology
Francesca M Bosisio, James S Wilmott, Nathalie Volders, Marjorie Mercier, Jasper Wouters, Marguerite Stas, Willeke Am Blokx, Daniela Massi, John F Thompson, Richard A Scolyer, Nicolas van Baren, Joost J van den Oord
Melanoma is not only one of the most immunogenic cancers but also one of the most effective cancers at subverting host immunity. The role of T lymphocytes in tumor immunity has been extensively studied in melanoma, whereas less is known about the importance of B lymphocytes. The effects of plasma cells (PCs), in particular, are still obscure. The aim of this study was to characterize pathological features and clinical outcome of primary cutaneous melanomas associated with PCs. Moreover, we investigated the origins of the melanoma-associated PCs...
April 2016: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
Francesca Salvianti, Claudio Orlando, Daniela Massi, Vincenzo De Giorgi, Marta Grazzini, Mario Pazzagli, Pamela Pinzani
Solid tumor release into the circulation cell-free DNA (cfDNA) and circulating tumor cells (CTCs) which represent promising biomarkers for cancer diagnosis. Circulating tumor DNA may be studied in plasma from cancer patients by detecting tumor specific alterations, such as genetic or epigenetic modifications. Ras association domain family 1 isoform A (RASSF1A) is a tumor suppressor gene silenced by promoter hypermethylation in a variety of human cancers including melanoma. The aim of the present study was to assess the diagnostic performance of a tumor-related methylated cfDNA marker in melanoma patients and to compare this parameter with the presence of CTCs...
2015: Frontiers in Molecular Biosciences
D Massi, D Brusa, B Merelli, C Falcone, G Xue, A Carobbio, R Nassini, G Baroni, E Tamborini, L Cattaneo, V Audrito, S Deaglio, M Mandalà
BACKGROUND: BRAF inhibitors (BRAFi) improve survival in metastatic melanoma patients (MMP) but the duration of clinical benefit is limited by development of drug resistance. Here, we investigated whether the expression of programmed death-ligand 1 (PD-L1) and the density of tumor-infiltrating mononuclear cells (TIMC) predict the occurrence of resistance, hence affecting the clinical outcome in BRAFi-treated MMP. METHODS: PD-L1 expression (cutoff 5%) was analyzed by immunohistochemistry with two different antibodies in BRAF(V600)-mutated formalin-fixed and paraffin-embedded samples from 80 consecutive MMP treated with BRAFi at a single institution...
September 2015: Annals of Oncology: Official Journal of the European Society for Medical Oncology
Daniela Massi, Carlo Tomasini, Rebecca Senetta, Milena Paglierani, Francesca Salvianti, Maria Elena Errico, Vittoria Donofrio, Paola Collini, Gabrina Tragni, Angela Rita Sementa, Franco Rongioletti, Renata Boldrini, Andrea Ferrari, Claudio Gambini, Maria Cristina Montesco
BACKGROUND: Diagnosis and proper management of atypical Spitz tumors in pediatric age are still controversial. OBJECTIVE: We sought to investigate the clinicopathological and molecular features of atypical Spitz tumors in patients aged 18 years or younger. METHODS: We performed a retrospective clinicopathological and fluorescence in situ hybridization study on 50 pediatric atypical Spitz tumors. RESULTS: Parameters that were significantly correlated with a diagnosis of atypical Spitz tumors over Spitz nevus included asymmetry, level IV/V, lack of maturation, solid growth, nuclear pleomorphism, high nuclear-cytoplasmic ratio, atypical and deep mitoses, and more than 6 mitoses/mm(2)...
January 2015: Journal of the American Academy of Dermatology
Raffaella Santi, Lisa Simi, Rossella Fucci, Milena Paglierani, Monica Pepi, Pamela Pinzani, Barbara Merelli, Marco Santucci, Gerardo Botti, Carmelo Urso, Daniela Massi
BACKGROUND: Mucosal melanomas (MM) represent a heterogeneous tumour population that exhibits site-specific molecular profiles. AIMS: In a multicentre retrospective study, we investigated KIT aberrations in primary anorectal (AR) melanomas compared with melanoma metastatic to the gastrointestinal (GI) tract. METHODS: Primary AR MM (n=31) and GI metastatic melanoma (n=27) were studied for KIT mutations on exons 11, 13, 17 and 18 by high-resolution melting analysis, direct sequencing and c-KIT expression by immunohistochemistry...
February 2015: Journal of Clinical Pathology
Daniela Massi, Lisa Simi, Elisa Sensi, Gianna Baroni, Gongda Xue, Cristian Scatena, Adele Caldarella, Pamela Pinzani, Gabriella Fontanini, Alessandra Carobbio, Carmelo Urso, Mario Mandalà
Testing for NRAS is now integral part in the assessment of metastatic melanoma patients because there is evidence that NRAS-mutated patients may be sensitive to MEK inhibitors, and RAS mutation is a common mechanism of acquired resistance during treatment with BRAF inhibitors. This study evaluated the sensitivity and specificity of immunohistochemical analysis using an N-Ras (Q61R) antibody to detect the presence of the NRASQ61R mutation in melanoma patients. A total of 98 primary cutaneous melanomas that have undergone examination of NRAS mutation were retrieved from a multicentric database...
April 2015: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
I Savarese, F Papi, A D'Errico, A Gori, M Grazzini, M Vannucchi, D Massi, V De Giorgi
An 85-year-old woman presented with a lesion on the sole of her right foot, which was histologically confirmed as acral lentiginous melanoma. Because of the large field involved and because the patient refused any invasive or painful treatment, topical treatment with imiquimod was commenced. At the 20-month follow-up, the patient was still continuing treatment with topical imiquimod, and no metastases to the lymph nodes or viscera were found, either clinically or in imaging studies. We believe that the success of the treatment cannot be explained only by the stimulation of the immune system induced by imiquimod...
January 2015: Clinical and Experimental Dermatology
D Massi, D Brusa, B Merelli, M Ciano, V Audrito, S Serra, R Buonincontri, G Baroni, R Nassini, D Minocci, L Cattaneo, E Tamborini, A Carobbio, E Rulli, S Deaglio, M Mandalà
BACKGROUND: Programmed cell death ligand 1 (PD-L1) is a cell surface molecule that plays a critical role in suppressing immune responses, mainly through binding of the PD-1 receptor on T lymphocytes. PD-L1 may be expressed by metastatic melanoma (MM). However, its clinical and biological significance remains unclear. Here, we investigated whether expression of PD-L1 in MM identifies a biologically more aggressive form of the disease, carrying prognostic relevance. PATIENTS AND METHODS: PD-L1 expression was analyzed by immunohistochemistry using two different antibodies in primary tumors and paired metastases from 81 melanoma patients treated at a single institution...
December 2014: Annals of Oncology: Official Journal of the European Society for Medical Oncology
V De Giorgi, I Savarese, A D'Errico, A Gori, F Papi, M Colombino, M Cristina Sini, I Stanganelli, G Palmieri, D Massi
BACKGROUND: Patients who develop cutaneous melanoma are at increased risk of developing a second primary melanoma. There are many aetiological reasons by which the risk of a second melanoma increases. Among others, genetic factors may contribute to modulating this risk. The risk of identifying a CDKN2A germline mutation increases with the number of primary melanomas and with the presence of familial history of melanoma. Patients with melanoma are especially encouraged to have regular follow-up visits with their dermatologist to perform clinical and dermatoscopic examination...
March 2015: Journal of the European Academy of Dermatology and Venereology: JEADV
Federica Scarfì, Massimiliano Galeone, Andrea Bassi, Meena Arunachalam, Daniela Massi, Elisa M Difonzo
No abstract text is available yet for this article.
September 2014: International Journal of Dermatology
Mario Mandalà, Barbara Merelli, Daniela Massi
RAS belongs to the guanosine 5'-triphosphate (GTP)-binding proteins' family, and oncogenic mutations in codons 12, 13, or 61 of RAS family occur in approximately one third of all human cancers with N-RAS mutations found in about 15-20% of melanomas. The importance of RAS signaling as a potential target in cancer is emphasized not only by the prevalence of RAS mutations, but also by the high number of RAS activators and effectors identified in mammalian cells that places the RAS proteins at the crossroads of several, important signaling networks...
November 2014: Critical Reviews in Oncology/hematology
Sandro Pasquali, Maria Cristina Montesco, Chiara Ginanneschi, Gianna Baroni, Clelia Miracco, Carmelo Urso, Fabio Mele, Anna Rita Lombardi, Pietro Quaglino, Laura Cattaneo, Stefania Staibano, Gerardo Botti, Paolo Visca, Marina Zannoni, Giuseppe Soda, Barbara Corti, Luca Pilloni, Luca Anselmi, Amelia Lissia, Margherita Vannucchi, Cristina Manieli, Daniela Massi
BACKGROUND: Scalp/neck melanomas have a poor prognosis, possibly because of a rich vascular supply that prompts tumor cells' dissemination. METHODS: We compared the accuracy of immunohistochemical (IHC) staining with morphology for the identification of lymphovascular invasion in 156 scalp/neck melanomas. We then analyzed the association of vessel invasion and density with pathological features and survival. RESULTS: IHC-detected lymphatic vessel invasion (LVI) and blood vessel invasion (BVI) were identified in 34...
November 2015: Head & Neck
Maria Colombino, MariaCristina Sini, Amelia Lissia, Vincenzo De Giorgi, Ignazio Stanganelli, Fabrizio Ayala, Daniela Massi, Corrado Rubino, Antonella Manca, Panagiotis Paliogiannis, Susanna Rossari, Serena Magi, Laura Mazzoni, Gerardo Botti, Mariaelena Capone, Marco Palla, Paolo A Ascierto, Antonio Cossu, Giuseppe Palmieri
BACKGROUND: Alterations in key-regulator genes of disease pathogenesis (BRAF, cKIT, CyclinD1) have been evaluated in patients with multiple primary melanoma (MPM). METHODS: One hundred twelve MPM patients (96 cases with two primary melanomas, 15 with three, and 1 with four) were included into the study. Paired synchronous/asynchronous MPM tissues (N=229) were analyzed for BRAF mutations and cKIT/CyclynD1 gene amplifications. RESULTS: BRAF mutations were identified in 109/229 (48%) primary melanomas, whereas cKIT and CyclinD1 amplifications were observed in 10/216 (5%) and 29/214 (14%) tumor tissues, respectively...
2014: Journal of Translational Medicine
Camilla Fusi, Serena Materazzi, Daiana Minocci, Vincenza Maio, Teresa Oranges, Daniela Massi, Romina Nassini
A subgroup of the transient receptor potential (TRP) channels, including vanilloid 1 (TRPV1), TRPV2, TRPV3, TRPV4, and TRP ankyrin 1 (TRPA1), is expressed in cutaneous peptidergic somatosensory neurons, and has been found in skin non-neuronal cells, such as keratinocytes. Different cancer cells express TRPs, where they may exert either pro- or antitumorigenic roles. Expression and function of TRPs in skin cancers have been, however, poorly investigated. Here, we have studied the distribution and expression of TRPs by immunohistochemistry and messenger RNA (mRNA) in human healthy skin and human keratinocytic tumors, including intraepidermal proliferative disorders (solar keratosis (SK) and Bowen's disease), and non-melanoma skin cancer (NMSC; basal cell and squamous cell carcinomas)...
September 2014: Journal of Investigative Dermatology
Cinzia Mazzini, Pamela Pinzani, Francesca Salvianti, Cristian Scatena, Milena Paglierani, Francesca Ucci, Mario Pazzagli, Daniela Massi
Uveal melanoma is one of the most deadly diseases in ophthalmology for which markers able to predict the appearance of metastasis are needed. The study investigates the role of circulating tumor cells (CTC) as a prognostic factor in this disease. We report the detection of circulating tumor cells by Isolation by Size of Epithelial Tumor cells (ISET) in a cohort of 31 uveal melanoma patients: we identified single CTCs or clusters of cells in 17 patients, while the control population, subjects with choroidal nevi, showed no CTC in peripheral blood...
2014: Cancers
Mario Mandalà, Daniela Massi
Cutaneous melanoma (CM) causes the greatest number of skin cancer-related deaths worldwide. Predicting CM prognosis is important to determine the need for further investigation, counseling of patients, to guide appropriate management (particularly the need for postoperative adjuvant therapy), and for assignment of risk status in groups of patients entering clinical trials. Since recurrence rate is largely independent from stages defined by morphological and morphometric criteria, there is a strong need for identification of additional robust prognostic factors to support decision-making processes...
March 2014: Virchows Archiv: An International Journal of Pathology
Giovanni Ponti, Cristel Ruini, Daniela Massi, Giovanni Pellacani, Aldo Tomasi, Milena Paglierani, Pietro Loschi, Stefania Seidenari
Although the 'gold standard' for melanoma diagnosis remains histopathological analysis, presently dermoscopists play a significant role in the diagnostic process. However, even a combined approach may not allow a clear-cut judgment on equivocal melanocytic lesions. Fluorescence in-situ hybridization (FISH) can offer assistance in the evaluation of chromosome abnormalities associated with malignancies, and its role is emerging in melanoma diagnosis. The aim of this study was to evaluate the diagnostic role of the FISH in the assessment of controversial lesions, defined as those lesions showing discrepancies between dermatoscopic and histological evaluations...
December 2013: Melanoma Research
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