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https://www.readbyqxmd.com/read/28634118/identification-of-chickpea-seed-proteins-resistant-to-simulated-in-vitro-human-digestion
#1
I C Ribeiro, C C Leclercq, N Simões, A Toureiro, I Duarte, J B Freire, M M Chaves, J Renaut, C Pinheiro
Proteins and peptides able to resist gastrointestinal digestion and reach the intestinal mucosa have the potential to influence human health. Chickpea (Cicer arietinum L.) seed proteins are able to resist cooking (86.9% total protein) and/or in vitro simulated human digestion (15.9% total protein resists soaking, cooking and digestion with pepsin and pancreatin). To identify and characterize proteins resisting digestion we made use of different MS methodologies. The efficiency of several proteases (trypsin, AspN, chymotrypsin and LysC) was tested, and two technologies were employed (MALDI-TOF-TOF and LC-nESI-MS/MS)...
June 17, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28633975/efficacy-of-inactivation-of-viral-contaminants-in-hyperimmune-horse-plasma-against-botulinum-toxin-by-low-ph-alone-and-combined-with-pepsin-digestion
#2
Amram Torgeman, Nurit Mador, Marina Dorozko, Aliza Lifshitz, Naomi Eschar, Moshe D White, Dana G Wolf, Eyal Epstein
Assuring viral safety of horse plasma-derived products is fundamental for ethical and regulatory reasons. We previously demonstrated the ability of pepsin digestion at low pH to inactivate West Nile and Sindbis viruses in horse plasma. The present study further examined the efficiency of pepsin digestion to inactivate four additional viruses: HSV-1 and BVDV (lipid-enveloped), BPV and Reo-3 (nonenveloped). These viruses were spiked into hyperimmunized horse plasma against botulinum toxin and subjected to low pH (3...
June 17, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28627065/evaluation-of-an-in%C3%A2-vitro-system-to-simulate-equine-foregut-digestion-and-the-influence-of-acidity-on-protein-and-fructan-degradation-in-the-horse-s-stomach
#3
S Strauch, B Wichert, J M Greef, D Hillegeist, A Zeyner, A Liesegang
The aim of this study was to improve an in vitro system in order to gather optimized information on the digestion of different forages in the horse's upper gastrointestinal tract. Therefore, foregut digestion of several forages was simulated in vitro (Part 1). The effect of different pH values on in vitro fructan degradation of two selected grasses (Part 2) was tested subsequently. Part 1: We hypothesized that our system produces representative results simulating digestive processes in the upper alimentary tract, but neglects microbial fermentation...
June 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28615865/epidemiological-studies-on-trichinellosis-in-pigs-sus-scofa-in-india
#4
Pranita Konwar, Balbir Bagicha Singh, Jatinder Paul Singh Gill
The present study was conducted to determine the prevalence of trichinellosis in domestic pigs (Sus scrofa) in India. A total of 319 tissue and 279 serum samples were collected from 279 slaughter pigs. The samples were collected from small slaughter shops located in Assam, Chandigarh (Union territory), Punjab and Uttarakhand states of the country. The presence of Trichinella spp. antibodies in serum was determined using a commercially available enzyme-linked immunosorbent assay (ELISA) kit and tissue samples were digested using the OIE recommended pepsin-HCl digestion method...
June 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28602552/intensity-fading-maldi-tof-mass-spectrometry-and-functional-proteomics-assignments-to-identify-protease-inhibitors-in-marine-invertebrates
#5
Giovanni Covaleda, Sebastian A Trejo, Emir Salas-Sarduy, Maday Alonso Del Rivero, Maria Angeles Chavez, Francesc X Aviles
Proteases and their inhibitors have become molecules of increasing fundamental and applicative value. Here we report an integrated strategy to identify and analyze such inhibitors from Caribbean marine invertebrates extracts by a fast and sensitive functional proteomics-like approach. The strategy works in three steps: i) multiplexed enzymatic inhibition kinetic assays, ii) Intensity Fading MALDI-TOF MS to establish a link between inhibitory molecules and the related MALDI signal(s) detected in the extract(s), and iii) ISD-CID-T(3) MS fragmentation on the parent MALDI signals selected in the previous step, enabling the partial or total top-down sequencing of the molecules...
June 5, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28601742/protein-tannic-acid-multilayer-films-a-multifunctional-material-for-microencapsulation-of-food-derived-bioactives
#6
Hooi Hong Lau, Regan Murney, Nikolai L Yakovlev, Marina V Novoselova, Su Hui Lim, Nicole Roy, Harjinder Singh, Gleb B Sukhorukov, Brendan Haigh, Maxim V Kiryukhin
The benefits of various functional foods are often negated by stomach digestion and poor targeting to the lower gastrointestinal tract. Layer-by-Layer assembled protein-tannic acid (TA) films are suggested as a prospective material for microencapsulation of food-derived bioactive compounds. Bovine serum albumin (BSA)-TA and pepsin-TA films demonstrate linear growth of 2.8±0.1 and 4.2±0.1nm per bi-layer, correspondingly, as shown by ellipsometry. Both multilayer films are stable in simulated gastric fluid but degrade in simulated intestinal fluid...
June 2, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28585488/is-pepsin-a-reliable-marker-of-laryngopharyngeal-reflux-a-systematic-review
#7
Christian Calvo-Henríquez, Alberto Ruano-Ravina, Pedro Vaamonde, Gabriel Martínez-Capoccioni, And Carlos Martín-Martín
Objective Laryngopharyngeal reflux (LPR) is a common illness of otolaryngology visits. Over the past few years, pepsin has become a promising marker of LPR. The objective of the present research is to analyze the existing literature using pepsin as a diagnostic tool of LPR through a systematic review. Data Sources PubMed (Medline), Trip Database, Cochrane Library, EMBASE, SUMsearch, and Web of Science. Review Methods The outcome assessed was the presence of pepsin in LPR patients. We included articles in which pepsin was studied in LPR patients (clinically suspected or with confirmed diagnosis)...
May 1, 2017: Otolaryngology—Head and Neck Surgery
https://www.readbyqxmd.com/read/28585158/properties-of-probiotics-kocuria-sm1-and-rhodococcus-sm2-isolated-from-fish-guts
#8
S M Sharifuzzaman, Hafizur Rahman, Dawn A Austin, B Austin
This study characterized probiotics Kocuria SM1 and Rhodococcus SM2, which were recovered from the intestinal microbiota of rainbow trout (Oncorhynchus mykiss, Walbaum). The cultures were Gram-positive, non-motile, catalase-positive and oxidase-negative cocci or rods. Cell multiplication of SM1 and SM2 was observed at 4-37 °C (45 °C for SM1), in 0-20% (w/v) NaCl and at pH 2-11. The viability was not affected when exposed to pepsin at pH 2.0 and 3.0, and pancreatin at pH 8.0. Neither isolates were chrome azurol S-positive for siderophore production...
June 5, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28583182/tissue-specific-bioactivity-of-soluble-tendon-derived-and-cartilage-derived-extracellular-matrices-on-adult-mesenchymal-stem-cells
#9
Benjamin B Rothrauff, Guang Yang, Rocky S Tuan
BACKGROUND: Biological scaffolds composed of tissue-derived extracellular matrix (ECM) can promote homologous (i.e., tissue-specific) cell differentiation through preservation of biophysical and biochemical motifs found in native tissues. Solubilized ECMs derived from decellularized tendon and cartilage have recently been promoted as tissue-specific biomaterials, but whether tissue-specific bioactivity is preserved following solubilization is unknown. This study explored the tissue-specific bioactivity of soluble decellularized tendon and cartilage ECMs on human bone marrow-derived mesenchymal stem cells (MSCs) presented across different culture microenvironments, including two-dimensional (2D) tissue culture plastic, aligned electrospun nanofibers, cell pellets, and cell-seeded photocrosslinkable hydrogels...
June 5, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28579027/molecular-detection-of-toxoplasma-gondii-in-the-slaughter-sheep-and-goats-from-north-india
#10
Deepali Kalambhe, J P S Gill, Balbir Bagicha Singh
Toxoplasma gondii is an obligate intracellular parasite that infects almost all the warm blooded animals, including human beings. The disease usually remains asymptomatic but is a serious concern for pregnant women, developing foetus and immuno-compromised individuals. We collected 400 cardiac/skeletal muscle tissue samples from slaughter sheep (177) and goat (223) intended for human consumption from Punjab, Uttar Pradesh and Chandigarh states/union territory in North India. The samples were pepsin-HCl digested and DNA was extracted from all the digested samples...
July 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28578031/biophysical-evaluation-of-milk-clotting-enzymes-processed-by-high-pressure
#11
Bruno Ricardo de Castro Leite Júnior, Alline Artigiani Lima Tribst, Nicholas J Grant, Rickey Y Yada, Marcelo Cristianini
High pressure processing (HPP) is able to promote changes in enzymes structure. This study evaluated the effect of HP on the structural changes in milk-clotting enzymes processed under activation conditions for recombinant camel chymosin (212MPa/5min/10°C), calf rennet (280MPa/20min/25°C), bovine rennet (222MPa/5min/23°C), and porcine pepsin (50MPa/5min/20°C) and under inactivation conditions for all enzymes (600MPa/10min/25°C) including the protease from Rhizomucor miehei. In general, it was found that the HPP at activation conditions was able to increase the intrinsic fluorescence of samples with high pepsin concentration (porcine pepsin and bovine rennet), increase significantly the surface hydrophobicity and induce changes in secondary structure of all enzymes...
July 2017: Food Research International
https://www.readbyqxmd.com/read/28576050/extraction-and-characterization-of-collagen-from-antarctic-and-sub-antarctic-squid-and-its-potential-application-in-hybrid-scaffolds-for-tissue-engineering
#12
Rui C G Coelho, Ana L P Marques, Sara M Oliveira, Gabriela S Diogo, Rogério P Pirraco, Joana Moreira-Silva, José C Xavier, Rui L Reis, Tiago H Silva, João F Mano
Collagen is the most abundant protein found in mammals and it exhibits a low immunogenicity, high biocompatibility and biodegradability when compared with others natural polymers. For this reason, it has been explored for the development of biologically instructive biomaterials with applications for tissue substitution and regeneration. Marine origin collagen has been pursued as an alternative to the more common bovine and porcine origins. This study focused on squid (Teuthoidea: Cephalopoda), particularly the Antarctic squid Kondakovia longimana and the Sub-Antarctic squid Illex argentinus as potential collagen sources...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28576004/preparation-of-poly-lactic-co-glycolic-acid-and-chitosan-composite-nanocarriers-via-electrostatic-self-assembly-for-oral-delivery-of-insulin
#13
Bin Xu, Guohua Jiang, Weijiang Yu, Depeng Liu, Yongkun Liu, Xiangdong Kong, Juming Yao
To improve insulin bioavailability and overcome multiple barriers for oral delivery of insulin, the composite nanocarriers (PLGA/FA-CS) prepared from poly(lactide-co-glycoside) (PLGA) and folic acid modified chitosan (FA-CS) were fabricated via electrostatic self-assembly method. The resultant composite nanocarriers exhibited low cytotoxicity against HT-29 cells and excellent stability against protein solution. The chemical stability of loaded insulin against digestive enzyme were established in presence of simulated gastric fluid (SGF) containing pepsin and simulated intestinal fluid (SIF) containing pancreatin, respectively...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28568309/effect-of-soybean-husk-supplementation-on-the-fecal-fermentation-metabolites-and-microbiota-of-dogs
#14
Htun Myint, Yu Iwahashi, Satoshi Koike, Yasuo Kobayashi
In vitro fermentation and in vivo feeding experiments were conducted to characterize the effects of soybean (Glycine max) husk on the fecal fermentation metabolites and microbiota of dogs. An in vitro fermentation study using feces from three Toy Poodle dogs (6.5 ± 3.5 months in age and 2.9 ± 0.4 kg in body weight) revealed that the fecal inoculum was able to ferment soybean husk (supplemented at 0.01 g/mL culture) and increased levels of short chain fatty acids (SCFA) and Bifidobacterium, irrespective of pre-digestion of the husk by pepsin and pancreatin...
June 1, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28566735/interaction-processes-of-ciprofloxacin-with-graphene-oxide-and-reduced-graphene-oxide-in-the-presence-of-montmorillonite-in-simulated-gastrointestinal-fluids
#15
Shuai Ma, Yang Si, Fei Wang, Lei Su, CongCong Xia, Jun Yao, Huilun Chen, Xingyu Liu
This study investigated the interaction processes of ciprofloxacin (CIP) with graphene oxide (GO) and reduced GO (rGO) in presence of montmorillonite (Mont) in simulated gastrointestinal fluids. The order of CIP adsorption affinity was rGO+Mont > GO+Mont > rGO+Mont+pepsin > rGO > GO+Mont+pepsin > Mont > Mont+pepsin > GO > rGO+pepsin > GO+pepsin in simulated gastric fluid. Mont enhanced the adsorption of CIP on GO and rGO due to hydrated Si species coating on GO and rGO in the simulated gastric fluid...
May 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28561587/protective-effects-of-functional-chicken-liver-hydrolysates-against-liver-fibrogenesis-antioxidation-anti-inflammation-and-antifibrosis
#16
Po-Ju Chen, Jung-Kai Tseng, Yi-Ling Lin, Yi-Hsieng Samuel Wu, Yi-Tse Hsiao, Jr-Wei Chen, Yi-Chen Chen
Via an assay using an Amino Acid Analyzer, pepsin-digested chicken liver hydrolysates (CLHs) contain taurine (365.57 ± 39.04 mg/100 g), carnosine (14.03 ± 1.98 mg/100 g), and anserine (151.58 ± 27.82 mg/100 g). This study aimed to evaluate whether CLHs could alleviate thioacetamide (TAA)-induced fibrosis. A dose of 100 mg TAA/kg BW significantly increased serum liver damage indices and liver cytokine contents. Cell infiltration and monocytes/macrophages in livers of TAA-treated rats were illustrated by the H&E staining and immunohistochemical analysis of cluster of differentiation 68 (CD68, ED1), respectively...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28547657/compartmentalized-expression-patterns-of-pancreatic-and-gastric-related-genes-in-the-alimentary-canal-of-the-ascidian-ciona-intestinalis-evolutionary-insights-into-the-functional-regionality-of-the-gastrointestinal-tract-in-olfactores
#17
Satoshi Nakayama, Michio Ogasawara
Many heterotrophic animals have a one-way alimentary canal that is essential for their nutrition and sequential steps of the digestive system, namely ingestion, digestion, absorption and elimination, are widely shared among bilaterians. Morphological, functional and molecular knowledge of the alimentary canal has been obtained in particular from mammalian research but the shared features and evolution of these aspects of the highly diverged alimentary canal in the animal kingdom are still unclear. We therefore investigate spatial gene expression patterns of pancreatic- and gastric-related molecules of ascidians (a sister group of vertebrates) with special reference to the functional regionality of the gastrointestinal tract...
May 25, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28546787/pepsinogen-c-expression-regulation-and-its-relationship-with-cancer
#18
REVIEW
Shixuan Shen, Jingyi Jiang, Yuan Yuan
Pepsinogen C (PGC) belongs to the aspartic protease family and is secreted by gastric chief cells. PGC could be activated to pepsin C and digests polypeptides and amino acids, but as a zymogen PGC's functions is unclear. In normal physiological conditions, PGC is initially detected in the late embryonic stage and is mainly expressed in gastric mucosa. The in situ expression of PGC in gastric mucosa is decreased considerably in the process of superficial gastritis → atrophic gastritis → gastric cancer (GC), proving that PGC is a comparatively ideal negative marker of GC...
2017: Cancer Cell International
https://www.readbyqxmd.com/read/28533197/incomplete-antibodies-may-reduce-abo-cross-match-incompatibility-a-pilot-study
#19
Mehmet Özen, Soner Yılmaz, Tülin Özkan, Yeşim Özer, Aliye Aysel Pekel, Asuman Sunguroğlu, Günhan Gürman, Önder Arslan
OBJECTIVE: Any erythrocyte transfusion among humans having type A or B blood groups is impossible due to antibodies causing fatal transfusion complications. A cross-match test is performed to prevent immune transfusion complications before transfusion. Our hypothesis is that the Fragment antibody (Fab) part of antibody (incomplete antibody) may be used to prevent an immune stimulus related to complete antibody. Therefore, we designed a pilot study to evaluate the effectiveness of these incomplete antibodies with using cross-match tests...
May 23, 2017: Turkish Journal of Haematology: Official Journal of Turkish Society of Haematology
https://www.readbyqxmd.com/read/28532019/characterization-of-bladder-acellular-matrix-hydrogel-with-inherent-bioactive-factors
#20
Dan Jiang, Jianwen Huang, Huili Shao, Xuechao Hu, Lujie Song, Yaopeng Zhang
Bladder acellular matrix (BAM) hydrogel may have great potential in tissue engineering due to outstanding biocompatibility and the presence of inherent bioactive factors in BAM. In this study, we prepared the BAM hydrogel by the method of enzymatic solubilization with pepsin and characterize the microrheological properties of the BAM precursor solution. The structures of the BAM hydrogel were characterized by scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC)...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
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