keyword
MENU ▼
Read by QxMD icon Read
search

Pepsin

keyword
https://www.readbyqxmd.com/read/28533197/incomplete-antibodies-may-reduce-abo-cross-match-incompatibility-a-pilot-study
#1
Mehmet Özen, Soner Yılmaz, Tülin Özkan, Yeşim Özer, Aliye Aysel Pekel, Asuman Sunguroğlu, Günhan Gürman, Önder Arslan
OBJECTIVE: Any erythrocyte transfusion among humans having type A or B blood groups is impossible due to antibodies causing fatal transfusion complications. A cross-match test is performed to prevent immune transfusion complications before transfusion. Our hypothesis is that the Fragment antibody (Fab) part of antibody (incomplete antibody) may be used to prevent an immune stimulus related to complete antibody. Therefore, we designed a pilot study to evaluate the effectiveness of these incomplete antibodies with using cross-match tests...
May 23, 2017: Turkish Journal of Haematology: Official Journal of Turkish Society of Haematology
https://www.readbyqxmd.com/read/28532019/characterization-of-bladder-acellular-matrix-hydrogel-with-inherent-bioactive-factors
#2
Dan Jiang, Jianwen Huang, Huili Shao, Xuechao Hu, Lujie Song, Yaopeng Zhang
Bladder acellular matrix (BAM) hydrogel may have great potential in tissue engineering due to outstanding biocompatibility and the presence of inherent bioactive factors in BAM. In this study, we prepared the BAM hydrogel by the method of enzymatic solubilization with pepsin and characterize the microrheological properties of the BAM precursor solution. The structures of the BAM hydrogel were characterized by scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC)...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28522132/salivary-pepsin-test-useful-and-simple-tool-for-the-laryngopharyngeal-reflux-diagnosis
#3
Luz Barona-Lleó, Claudia Duval, Rafael Barona-de Guzmán
INTRODUCTION AND OBJECTIVES: Laryngopharyngeal Reflux (LPR) is a disease characterized by the presence of symptoms, signs and tissue damage caused by retrograde flow of gastric contents to the upper aerodigestive tract. It represents up to 10% of otolaryngology consultations. The aim of the study is to describe the findings obtained by applying the salivary pepsin test (PEP-test) in a sample of patients with the clinical suspicion of LPR. MATERIAL AND METHODS: Our descriptive clinical study included 142 subjects with symptoms suggestive of LPR and a score above 13 on the RSI scale...
May 15, 2017: Acta Otorrinolaringológica Española
https://www.readbyqxmd.com/read/28506800/behaviour-of-silver-nanoparticles-in-simulated-saliva-and-gastrointestinal-fluids
#4
Lucie Pinďáková, Věra Kašpárková, Kristina Kejlová, Markéta Dvořáková, Daniel Krsek, Dagmar Jírová, Lucie Kašparová
Continuously increasing application of silver nanoparticles (AgNPs) requires information on their safety and performance under biological conditions. Assessment of AgNPs in biological systems is also related to availability of robust toxicological methods for evaluation of toxic potential of AgNPs and information on their physicochemical state. Silver nanoparticles were subjected to action of simulated saliva, gastric and intestinal fluids, appropriately supplemented with digestive enzymes pepsin or pancreatin...
May 12, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28503709/morphological-and-molecular-characterization-of-sarcocystis-arctica-like-sarcocysts-from-the-arctic-fox-vulpes-lagopus-from-alaska-usa
#5
Camila K Cerqueira-Cézar, Peter C Thompson, Shiv Kumar Verma, Joseph Mowery, Rafael Calero-Bernal, Fernando H Antunes Murata, David R Sinnett, Caroline Van Hemert, Benjamin M Rosenthal, Jitender P Dubey
The muscles of herbivores commonly harbor sarcocysts of parasites belonging to species in the genus Sarcocystis, but such muscle parasites are rare in carnivores. Here, we report Sarcocystis arctica-like sarcocysts in muscles of Arctic foxes (Vulpes lagopus) from Alaska, USA, for the first time. The tongues of 56 foxes were examined for Sarcocystis infection using several methods. Sarcocystis bradyzoites were detected in pepsin digests of 13 (23.2%), and sarcocysts were found in histological sections stained with hematoxylin and eosin (HE) of 9 (16...
May 15, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28497137/identification-of-two-novel-antioxidant-peptides-from-edible-bird-s-nest-aerodramus-fuciphagus-protein-hydrolysates
#6
Masomeh Ghassem, Keizo Arihara, Salimeh Mohammadi, Norrakiah Abdullah Sani, Abdul Salam Babji
Edible bird's nest (EBN) is widely consumed as a delicacy and traditional medicine amongst the Chinese. In the present study, for the first time, the antioxidant properties of an EBN pepsin-trypsin hydrolysate of the swiftlet species Aerodramus fuciphagus and its ultrafiltration fractions were investigated. Thirteen peptides with molecular weights between 514.29 and 954.52 Da were identified in the EBN fraction with the use of mass spectrometry. Two novel pentapeptides Pro-Phe-His-Pro-Tyr and Leu-Leu-Gly-Asp-Pro, corresponding to f134-138 and f164-168 of cytochrome b of A...
May 24, 2017: Food & Function
https://www.readbyqxmd.com/read/28495303/the-characteristics-of-salivary-pepsin-in-patients-with-severe-motor-and-intellectual-disabilities
#7
Naoki Hashizume, Suguru Fukahori, Kimio Asagiri, Shinji Ishii, Nobuyuki Saikusa, Naruki Higashidate, Motomu Yoshida, Daisuke Masui, Saki Sakamoto, Yoshiaki Tanaka, Minoru Yagi, Yushiro Yamashita
PURPOSE: The aim of the present study was to determine the utility of measuring the salivary pepsin level (SPL) as an objective assessment of gastroesophageal reflux disease (GERD) in severe motor and intellectual disabilities (SMID) patients. SUBJECTS AND METHODS: This prospective study included 26 SMID patients who underwent simultaneous 24-h multichannel intraluminal impedance pH measurement (pH/MII) and SPL evaluation. The enrolled patients were divided into GERD (+) or GERD (-) groups according to the pH/MII findings...
May 8, 2017: Brain & Development
https://www.readbyqxmd.com/read/28494009/postnatal-changes-and-sexual-dimorphism-in-collagen-expression-in-mouse-skin
#8
Koji Y Arai, Takuya Hara, Toyofumi Nagatsuka, Chikako Kudo, Sho Tsuchiya, Yoshihiro Nomura, Toshio Nishiyama
To investigate sexual dimorphism and postnatal changes in skin collagen expression, mRNA levels of collagens and their regulatory factors in male and female skin were examined during the first 120 days of age by quantitative realtime PCR. Levels of mRNAs encoding extracellular matrices did not show any differences between male and female mice until day 15. Col1a1 and Col1a2 mRNAs noticeably increased at day 30 and remained at high levels until day 120 in male mice, while those in female mice remained at low levels during the period...
2017: PloS One
https://www.readbyqxmd.com/read/28486810/a-facile-strategy-to-prepare-enzyme-responsive-mussel-mimetic-coating-for-drug-delivery-based-on-mesoporous-silica-nanoparticles
#9
Chunlin Hu, Xinling Wang, Ping Huang, Zhen Zheng, Zhibiao Yang
Surface functional mesoporous silicon nanoparticles (MSNs) have been used widely as promosing materials for drug delivery. Herein, we reported a facile strategy to construct MSNs coated by enzyme-resposive polylysine-dopamine (PLDA) films through self-polymerization of dopamine derivative lysine-dopamine, in which the drug could be loaded and delivered efficiently. In details, RhB or DOX was used as a drug model and loaded in functional MSNs via one-pot procedure among MSNs, drug and lysine-dopamine (LDA) under basic condition...
May 9, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28486778/efficacy-of-thermoresponsive-photocrosslinkable-hydrogels-derived-from-decellularized-tendon-and-cartilage-extracellular-matrix-for-cartilage-tissue-engineering
#10
Benjamin B Rothrauff, Luca Coluccino, Riccardo Gottardi, Luca Ceseracciu, Silvia Scaglione, Luca Goldoni, Rocky S Tuan
Tissue engineering using adult mesenchymal stem cells (MSCs), a promising approach for cartilage repair, is highly dependent on the nature of the matrix scaffold. Thermoresponsive, photocrosslinkable hydrogels were fabricated by functionalizing pepsin-soluble decellularized tendon and cartilage extracellular matrices (ECM) with methacrylate groups. Methacrylated gelatin hydrogels served as controls. When seeded with human bone marrow mesenchymal stem cells (MSCs) and cultured in chondrogenic medium, methacrylated ECM hydrogels experienced less cell-mediated contraction, as compared against non-methacrylated ECM hydrogels...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28475143/hydrolysates-of-fish-skin-collagen-an-opportunity-for-valorizing-fish-industry-byproducts
#11
María Blanco, José Antonio Vázquez, Ricardo I Pérez-Martín, Carmen G Sotelo
During fish processing operations, such as skinning and filleting, the removal of collagen-containing materials can account for up to 30% of the total fish byproducts. Collagen is the main structural protein in skin, representing up to 70% of dry weight depending on the species, age and season. It has a wide range of applications including cosmetic, pharmaceutical, food industry, and medical. In the present work, collagen was obtained by pepsin extraction from the skin of two species of teleost and two species of chondrychtyes with yields varying between 14...
May 5, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28462718/rubiscolins-highly-potent-peptides-derived-from-plant-proteins
#12
Renata Perlikowska, Anna Janecka
Rubiscolins are two naturally occurring, linear peptides, isolated from the pepsin digests of D-ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) from spinach leaves. Their sequences are as follows: Tyr-Pro-Leu-Asp-Leu (YPLDL, rubiscolin-5) and Tyr-Pro-Leu-Asp-Leu-Phe (YPLDLF, rubiscolin-6, also known as rubixyl). Though their structure does not resemble typical opioid peptides, rubiscolins were found to exhibit high affinity and selectivity for the delta opioid receptor and an antinociceptive effect in mice after oral administration...
April 26, 2017: Mini Reviews in Medicinal Chemistry
https://www.readbyqxmd.com/read/28460976/in-vitro-versus-in-vivo-protein-digestibility-techniques-for-calculating-pdcaas-protein-digestibility-corrected-amino-acid-score-applied-to-chickpea-fractions
#13
Olga Luisa Tavano, Valdir Augusto Neves, Sinézio Inácio da Silva Júnior
Seven different in vitro methods to determine the protein digestibility for chickpea proteins were considered and also the application of these methodologies for calculating PDCAAS (protein digestibility-corrected amino acid score), seeking their correlations with the in vivo methodology. In vitro digestibility of raw and heated samples were determined using pepsin-pancreatin hydrolysis, considering soluble nitrogen via Kjeldahl (ppKJ) and hydrolysed peptide linkages using trinitrobenzenesulfonic acid and o-phthaldialdehyde...
November 2016: Food Research International
https://www.readbyqxmd.com/read/28460964/hypoallergenic-hydrolysates-of-egg-white-proteins-modulate-allergen-responses-induced-ex-vivo-on-spleen-cells-from-sensitized-mice
#14
Daniel Lozano-Ojalvo, Elena Molina, Rosina López-Fandiño
This study describes the in vivo allergenicity of enzymatic hydrolysates of egg white proteins (ovalbumin, lysozyme and ovomucoid) and explores the possibility that they could modulate T cell cytokine responses to egg allergens ex vivo, using splenocytes from BALB/c mice sensitized to individual egg proteins or to their mixtures in different proportions. The hydrolysate of ovalbumin with pepsin could be regarded as a good candidate for peptide-based immunotherapy on the grounds of its reduced ability to trigger allergic symptoms in a passive cutaneous anaphylaxis assay and its potential to reduce Th2 responses (release of IL-4 and IL-5) induced by egg allergens in the spleen cell cultures, but also to enhance Th1 responses (release of TNF-α and IFN-γ)...
November 2016: Food Research International
https://www.readbyqxmd.com/read/28456399/prior-lactose-glycation-of-caseinate-via-the-maillard-reaction-affects-in-vitro-activities-of-the-pepsin-trypsin-digest-toward-intestinal-epithelial-cells
#15
X P Wang, X H Zhao
The well-known Maillard reaction in milk occurs between lactose and milk proteins during thermal treatment, and its effects on milk nutrition and safety have been well studied. A lactose-glycated caseinate was prepared via this reaction and digested using 2 digestive proteases, pepsin and trypsin. The glycated caseinate digest was assessed for its in vitro activities on rat intestinal epithelial cells in terms of growth proliferation, anti-apoptotic effect, and differentiation induction using caseinate digest as reference, to verify potential effects of the Maillard reaction on these activities of caseinate digest to the cells...
April 26, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28441786/application-of-pineapple-juice-in-the-fish-digestion-process-for-carcinogenic-liver-fluke-metacercaria-collection
#16
Panupan Sripan, Ratchadawan Aukkanimart, Thidarut Boonmars, Pranee Sriraj, Jiraporn Songsri, Parichart Boueroy, Sukhonthip Khueangchaingkhwang, Benjamabhorn Pumhirunroj, Atchara Artchayasawat
Pepsin is common digestive enzyme used for fish digestion in the laboratory to collect trematode metacercariae. In a field study, to survey the infected fish is needed a huge yield of pepsin and it is very expensive. Therefore, our purpose of this study was to investigate the candidate enzyme from pineapple juice which has a digestive enzyme called bromelain, a mixture of proteolytic enzymes, to digest fish in order to harvest metacercariae. Fish were divided into 2 groups: one group in which metacercariae were harvested using acid pepsin as a control and other groups in which the fish was digested using fresh pineapple juices...
March 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28441731/polyphenolic-compounds-and-digestive-enzymes-in-vitro-non-covalent-interactions
#17
REVIEW
Alejandra I Martinez-Gonzalez, Ángel G Díaz-Sánchez, Laura A de la Rosa, Claudia L Vargas-Requena, Ismael Bustos-Jaimes, And Emilio Alvarez-Parrilla
The digestive enzymes-polyphenolic compounds (PCs) interactions behind the inhibition of these enzymes have not been completely studied. The existing studies have mainly analyzed polyphenolic extracts and reported inhibition percentages of catalytic activities determined by UV-Vis spectroscopy techniques. Recently, pure PCs and new methods such as isothermal titration calorimetry and circular dichroism have been applied to describe these interactions. The present review focuses on PCs structural characteristics behind the inhibition of digestive enzymes, and progress of the used methods...
April 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28437072/the-single-disulfide-directed-%C3%AE-hairpin-fold-dynamics-stability-and-engineering
#18
Balasubramanyam Chittoor, Bankala Krishnarjuna, Rodrigo A V Morales, Christopher A MacRaild, Maiada Sadek, Eleanor W W Leung, Samuel D Robinson, Michael W Pennington, Raymond S Norton
Grafting bioactive peptide sequences onto small cysteine-rich scaffolds is a promising strategy for enhancing their stability and value as novel peptide-based therapeutics. However, correctly folded disulfide-rich peptides can be challenging to produce by either recombinant or synthetic means. The single disulfide-directed β-hairpin (SDH) fold, first observed in contryphan-Vc1, provides a potential alternative to complex disulfide-rich scaffolds. We have undertaken recombinant production of full-length contryphan-Vc1 (rCon-Vc1[Z1Q]) and a truncated analogue (rCon-Vc11-22[Z1Q]), analyzed the backbone dynamics of rCon-Vc1[Z1Q], and probed the conformational and proteolytic stability of these peptides to evaluate the potential of contryphan-Vc1 as a molecular scaffold...
May 2, 2017: Biochemistry
https://www.readbyqxmd.com/read/28430776/aminoazo-dye-protein-adduct-enhances-inhibitory-effect-on-digestibility-and-damages-to-gastro-duodenal-hepatic-axis
#19
Li-Yun Lin, Chiung-Chi Peng, Yeh Chen, Boa-Chan Huang, Chun Chao Chang, Robert Y Peng
4-Dimethylaminoazobenzene (DAB, methyl yellow, or butter yellow), a human carcinogen, has been banned for use in foods since 1988. In 2014, DAB adulteration in Tofu occurred in Taiwan. We hypothesize that DAB can form [DAB•SBP]adduct adduct with soybean protein (SBP) which could damage Gastro-Duodenal-Hepatic axis. Sprague-Dawley rats gavage fed [DAB•SBP]adduct adduct revealed severely reduced body weight and damaged duodenum, liver, hepatic mitochondria, and spleen. Hepatic levels of glutathione and ATP were severely reduced...
2017: PloS One
https://www.readbyqxmd.com/read/28426073/ameliorative-effects-of-pepsin-digested-chicken-liver-hydrolysates-on-development-of-alcoholic-fatty-livers-in-mice
#20
Yi-Ling Lin, Szu-Yun Tai, Jr-Wei Chen, Chung-Hsi Chou, Shih-Guei Fu, Yi-Chen Chen
With developments in economics and increasing work loads, alcohol abuse becomes more and more severe, leading to occurrences of alcoholic liver disease (ALD). Pepsin-digested chicken liver hydrolysates (CLHs) contain high amounts of glutamic acid, leucine, lysine, and alanine while the contents of taurine, anserine, and carnosine are also elevated after pepsin hydrolyzation. The objectives of this study were to evaluate the protective effects of CLHs against chronic alcohol consumption. The results indicated that the enlarged (p < 0...
May 24, 2017: Food & Function
keyword
keyword
100568
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"