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https://www.readbyqxmd.com/read/29147091/evaluation-of-the-digestibility-of-korean-hanwoo-beef-cuts-using-the-in-vitro-physicochemical-upper-gastrointestinal-system
#1
Ji-Hye Jeon, Michelle Yoo, Tae-Hwan Jung, Woo-Min Jeon, Kyoung-Sik Han
The aim of this study was to investigate the digestibility of different Korean Hanwoo beef cuts using an in vitro digestion model, in vitro physicochemical upper gastrointestinal system (IPUGS). The four most commonly consumed cuts - tenderloin, sirloin, brisket and flank, and bottom round - were chosen for this study. Beef samples (75 g) were cooked and ingested into IPUGS, which was composed of mouth, esophagus, and stomach, thereby simulating the digestion conditions of humans. Digested samples were collected every 15 min for 4 h of simulation and their pH monitored...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/29143646/validation-of-immune-complex-dissociation-methods-for-use-with-heartworm-antigen-tests
#2
Melissa J Beall, Andrea Arguello-Marin, Jan Drexel, Jiayou Liu, Ramaswamy Chandrashekar, A Rick Alleman
BACKGROUND: Antigen testing is routinely used to diagnose canine Dirofilaria immitis infections. Immune complex dissociation (ICD) methods, which were employed in the original heartworm antigen tests to release antigen that was bound by endogenous canine antibodies, were discontinued with improvements in assay reagents. The purpose of this study was to evaluate different ICD methods for detection of heartworm antigen by microtiter plate ELISA and assess the performance in samples from pet dogs...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29135822/premature-infants-have-lower-gastric-digestion-capacity-for-human-milk-proteins-than-term-infants
#3
Veronique Demers-Mathieu, Yunyao Qu, Mark A Underwood, Robyn Borghese, David Charles Dallas
OBJECTIVES: Whether premature infants have lower gastric protein digestive capacity than term infants and the extent to which human milk proteases contribute to overall gastric digestion are unknown and were investigated in this study. METHODS: Human milk and infant gastric samples were collected from 16 preterm (24-32 wk gestational age (GA)) and 6 term (38-40 wk GA) mother-infant pairs within a range of 5-42 days postnatal age. For each pair, an aliquot of human milk was adjusted to pH 4...
November 10, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/29115567/preparation-and-characterization-of-acellular-adipose-tissue-matrix-using-a-combination-of-physical-and-chemical-treatments
#4
Mei Song, Yi Liu, Ling Hui
Decellularized adipose extracellular matrix (ECM) has been used in the clinic to support the regeneration of adipose tissues. The methods used to produce adipose tissue ECM scaffolds exhibit distinct effects upon the structural and functional components of the resultant scaffold material. The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion...
October 24, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29114652/pepsin-egg-white-hydrolysate-ameliorates-metabolic-syndrome-in-high-fat-high-dextrose-fed-rats
#5
S Moreno-Fernández, M Garcés-Rimón, C González, J A Uranga, V López-Miranda, G Vera, M Miguel
The aim of this study was to examine the effect of a pepsin egg white hydrolysate (EWH) on metabolic complications using a high-fat/high-dextrose diet-induced Metabolic Syndrome (MetS) experimental model. Male Wistar rats were divided into 4 groups which received: standard diet and water (C), standard diet and a solution with 1 g kg(-1) day(-1) of EWH (CH), high-fat/high-dextrose diet and water (MS), and high-fat/high-dextrose diet and a solution with 1 g kg(-1) day(-1) of EWH (MSH). EWH consumption normalized body weight gain; abdominal obesity and peripheral neuropathy developed in MetS animals, and adipose tissue and liver weight, as well as plasma glucose were reduced...
November 8, 2017: Food & Function
https://www.readbyqxmd.com/read/29108629/spontaneous-pepsin-c-catalyzed-activation-of-human-pepsinogen-c-in-transgenic-rice-cell-suspension-culture-production-and-characterization-of-human-pepsin-c
#6
Md Reyazul Islam, Nan-Sun Kim, Jae-Wan Jung, Hyo-Boon Kim, So-Chon Han, Moon-Sik Yang
A human pepsinogen C (hPGC) gene was synthesized with rice-optimized codon usage and cloned into a rice expression vector containing the promoter, signal peptide, and terminator derived from the rice α-amylase 3D (Ramy3D) gene. In addition, a 6-His tag was added to the 3' end of the synthetic hPGC gene for easy purification. The plant expression vector was introduced into rice calli (Oryza sativa L. cv. Dongjin) mediated by Agrobacterium tumefaciens. The integration of the hPGC gene into the chromosome of the transgenic rice callus and hPGC expression in transgenic rice cell suspensions was verified via genomic DNA polymerase chain reaction amplification and Northern blot analysis...
January 2018: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/29098470/lysine-and-methionine-supplementation-ameliorates-high-inclusion-of-soybean-meal-inducing-intestinal-oxidative-injury-and-digestive-and-antioxidant-capacity-decrease-of-yellow-catfish
#7
Jun Jiang, Shangxiao Xu, Lin Feng, Yang Liu, Weidan Jiang, Pei Wu, Yan Wang, Ye Zhao, Xiaoqiu Zhou
The yellow catfish Pelteobagrus fulvidraco with initial average weight 16.6 ± 0.17 g were fed three extruded diets for 56 days. Fish meal (FM) diet was formulated as the normal control with 380 g FM and 200 g soybean meal (SBM) kg(-1) diet. The SBM diet was prepared with 220 g FM and 360 g SBM kg(-1) diet without Lys or Met supplementation. The SBM supplement (SBMS) diet was similar to SBM diet and supplemented with Lys and Met to ensure their levels similar to FM diet. The results showed fish fed SBM diet had lower percent weight gain and specific growth rate than the other two groups (P < 0...
November 2, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29096438/exploration-of-potentially-bioactive-peptides-generated-from-the-enzymatic-hydrolysis-of-hempseed-proteins
#8
Gilda Aiello, Carmen Lammi, Giovanna Boschin, Chiara Zanoni, Anna Arnoldi
The seed of industrial hemp is an underexploited protein source. In view of a possible use in functional foods, a hempseed protein concentrate was hydrolyzed with pepsin, trypsin, pancreatin, or a mixture of these enzymes. A detailed peptidomic analysis using data-dependent acquisition showed that the numbers of peptides identified ranged from 90 belonging to 33 parent proteins in the peptic hydrolysate to 9 belonging to 6 proteins in the pancreatin digest. The peptic and tryptic hydrolysates resulted to be the most efficient inhibitors of 3-hydroxymethyl-coenzyme A reductase activity, when tested on the catalytic domain of the enzyme...
November 2, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29092869/testing-placement-of-gastric-feeding-tubes-in-infants
#9
Norma A Metheny, Ann Pawluszka, Melanie Lulic, Leslie J Hinyard, Kathleen L Meert
BACKGROUND: Inadvertent positioning of a nasogastric tube in the lung can cause serious complications, so identifying methods to detect improperly inserted tubes is imperative. OBJECTIVES: To compare the sensitivity, specificity, and negative and positive predictive values of 4 pH cut points (< 4.0, < 4.5, < 5.0, and < 5.5) in differentiating gastric and tracheal aspirates under various treatment conditions and to explore the utility of a pepsin assay for distinguishing between gastric and tracheal aspirates...
November 2017: American Journal of Critical Care: An Official Publication, American Association of Critical-Care Nurses
https://www.readbyqxmd.com/read/29091918/software-aided-approach-to-investigate-peptide-structure-and-metabolic-susceptibility-of-amide-bonds-in-peptide-drugs-based-on-high-resolution-mass-spectrometry
#10
Tatiana Radchenko, Andreas Brink, Yves Siegrist, Christopher Kochansky, Alison Bateman, Fabien Fontaine, Luca Morettoni, Ismael Zamora
Interest in using peptide molecules as therapeutic agents due to high selectivity and efficacy is increasing within the pharmaceutical industry. However, most peptide-derived drugs cannot be administered orally because of low bioavailability and instability in the gastrointestinal tract due to protease activity. Therefore, structural modifications peptides are required to improve their stability. For this purpose, several in-silico software tools have been developed such as PeptideCutter or PoPS, which aim to predict peptide cleavage sites for different proteases...
2017: PloS One
https://www.readbyqxmd.com/read/29090713/pepsin-pancreatin-hydrolysis-reduced-the-ability-of-lunasin-enriched-material-to-inhibit-activation-of-the-inflammasomes-in-thp-1-human-macrophages
#11
Samuel James Price, Philipus Pangloli, Vermont P Dia
Inflammation caused by the NLRP3 inflammasome has been linked to many diseases. Lunasin is a bioactive peptide from soybeans with reported anti-inflammatory properties. The objective of this work was to determine the effect of pepsin-pancreatin hydrolysis (PPH) on the ability of lunasin-enriched preparation (LEP) to inhibit inflammasome activation in differentiated THP-1 human macrophages. THP-1 macrophages were treated with different concentrations of LEP (0.0625 to 0.25 mg mL(-1)), primed with 1 μg mL(-1) lipopolysaccharide for 6 h and activated by 5 mM adenosine triphosphate for 1 h...
November 1, 2017: Food & Function
https://www.readbyqxmd.com/read/29089856/pepsin-assisted-transglutaminase-modification-of-%C3%A2-functional-properties-of-a-protein-isolate-obtained-%C3%A2-from-industrial-sunflower-meal
#12
Petya Ivanova, Vesela I Chalova, Hristo Kalaydzhiev, Mariana Perifanova-Nemska, Turid Rustad, Lidia Koleva
The utilization of industrial sunflower meal to produce protein-rich products for the food industry is an alternative approach for better and more efficient use of this agricultural by-product. Sunflower meal proteins possess specific functional properties, which however need improvement to broaden their potential as supplements for delivering high--quality products for human nutrition. The aim of the study is to evaluate the combined influence of low-degree pepsin hydrolysis and transglutaminase (TG) modification on industrial sunflower meal protein isolate functionality at pH=2 to 10...
September 2017: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/29083493/bovine-hemoglobin-derived-peptide-asn-phe-gly-lys-inhibits-pancreatic-cancer-cells-metastasis-by-targeting-secreted-hsp90%C3%AE
#13
Yu Wang, Ting Zhang, Hongyi Zhang, Haixia Yang, Yanju Li, Yiqun Jiang
Pancreatic cancer is a digestive system malignant carcinoma with poor prognosis. The majority of patients are diagnosed with metastatic disease, which is also the leading cause of pancreatic cancer death. The aim of this study was to investigate the antimetastatic effect of Asn-Phe-Gly-Lys (NFGK), a tetrapeptide derived from bovine hemoglobin pepsin hydrolysate, on human pancreatic cancer cell line MIAPaCa-2. Wound healing assay and transwell invasion assay results showed that NFGK inhibited MIAPaCa-2 cell migration and invasion dose-dependently...
October 30, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/29082478/production-and-characterization-of-f-ab-2-fragments-obtained-by-enzymatic-digestion-from-murine-anti-mrsa-pbp2a-monoclonal-antibodies
#14
Anna Erika Vieira de Araujo, Natalia Plinio de Souza, Alvaro Paiva Braga de Sousa, Flavio Alves Lara, Jose Procopio Moreno Senna
Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are a worldwide health problem. In a previous study, a murine monoclonal antibody (mMAB), capable of binding to PBP2a within MRSA strains, was generated. F(ab')2 antibody fragments are widely described in the literature as immunochemical tools and reagents for diagnostics and therapeutics, particularly because of their low immunogenicity and rapid pharmacokinetics. In this study, F(ab')2 fragments from mMAB were generated by enzymatic digestion, using pepsin...
October 30, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29080817/production-and-structural-characterization-of-exopolysaccharides-from-newly-isolated-probiotic-lactic-acid-bacteria
#15
Yousra Abid, Angela Casillo, Houda Gharsallah, Ichrak Joulak, Rosa Lanzetta, Maria Michela Corsaro, Hamadi Attia, Samia Azabou
In this work, four exopolysaccharide-producing lactic acid bacteria (LAB) strains, newly isolated from Tunisian spontaneously fermented foods and beverages, namely bovine and turkey meat sausages (BMS and TMS), date palm sap (DPS) and cow milk (CM), were identified as Leuconostoc citreum-BMS, Leuconostoc mesenteroides-TMS, Pediococcus pentosaceus-DPS and Leuconostoc pseudomesenteroides-CM, respectively. The isolated strains showed the ability to withstand simulated human gastrointestinal (GI) tract conditions (low pH, lysozyme, bile salts, pepsin and pancreatin) and showed high surface hydrophobicity (79-90%), besides their ability to act against Escherichia coli and Listeria monocytogenes and to produce exopolysaccharides (EPS)...
October 25, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29078848/partially-purified-gloriosa-superba-peptides-inhibit-colon-cancer-cell-viability-by-inducing-apoptosis-through-p53-upregulation
#16
Prapaphan Budchart, Ariya Khamwut, Chomdao Sinthuvanich, Sunanta Ratanapo, Yong Poovorawan, Nattanan Panjaworayan T-Thienprasert
BACKGROUND: Colon cancer is a major health problem worldwide. Available treatments such as surgery, chemotherapy, radiation and anticancer drugs are limited due to stage of cancer, side effects and altered biodistribution. The use of peptides extracted from natural products has appeared as a potential therapy. Gloriosa superba is known to contain colchicine and other alkaloids with anticancer activity. However, these peptides contained within the extracts have not been studied. This study, therefore, focuses on an investigation of anti-colon cancer activity from a partially purified protein hydrolysate of G superba rhizome...
October 2017: American Journal of the Medical Sciences
https://www.readbyqxmd.com/read/29076531/topical-protection-of-mice-laryngeal-mucosa-using-the-natural-product-cashew-gum
#17
Aline A Figueiredo, Ana P M Santana, Lucas A D Nicolau, Francisco J Batista-Lima, Deysi V T Wong, Larisse T Lucetti, Gabriela L P Batista, Natália A Caminha, Jand V R Medeiros, Durcilene A Silva, José R S A Leite, Armênio A Santos, Pedro M G Soares, Daniel Sifrim, Marcellus H L P Souza
OBJECTIVES/HYPOTHESIS: Evaluate the effect of in vitro exposure of mice laryngeal mucosa to solutions that simulated human gastric juice and to assess the topical protective effect of cashew gum on mice laryngeal mucosal integrity in vitro. STUDY DESIGN: Animal study. METHODS: Murine (Swiss) laryngeal samples were mounted in Ussing chambers. The luminal side of biopsies was exposed to solutions of different acidity with or without pepsin and/or taurodeoxycholic acid (TDC)...
October 27, 2017: Laryngoscope
https://www.readbyqxmd.com/read/29072742/in-silico-modeling-of-protein-hydrolysis-by-endoproteases-a-case-study-on-pepsin-digestion-of-bovine-lactoferrin
#18
Alberto Tonda, Anita Grosvenor, Stefan Clerens, Steven Le Feunteun
This paper presents a novel model of protein hydrolysis and release of peptides by endoproteases. It requires the amino-acid sequence of the protein substrate to run, and makes use of simple Monte-Carlo in silico simulations to qualitatively and quantitatively predict the peptides that are likely to be produced during the course of the proteolytic reaction. In the present study, the model is applied to the case of pepsin, the gastric protease. Unlike pancreatic proteases, pepsin has a low substrate specificity and therefore displays a stochastic behavior that is particularly challenging to model and predict...
October 26, 2017: Food & Function
https://www.readbyqxmd.com/read/29072674/comparison-of-impedance-and-pepsin-detection-in-the-laryngeal-mucosa-to-determine-impedance-values-that-indicate-pathological-laryngopharyngeal-reflux
#19
Martin Formánek, Debora Jančatová, Pavel Komínek, Radoslava Tomanová, Karol Zeleník
OBJECTIVE: Recently, a 24-h impedance was used to detect laryngopharyngeal reflux (LPR). However, not every case of LPR is pathological. Thus, pathological pharyngeal impedance values need to be clearly established to diagnose pathological LPR. The aim of our study was to establish pathological 24-h pharyngoesophageal impedance/pH values for the diagnosis of LPR. METHODS: The study was conducted in a tertiary care setting. A total of 30 patients who were referred to microlaryngoscopy for a laryngeal pathology that might be caused by LPR were included in this prospective study...
October 26, 2017: Clinical and Translational Gastroenterology
https://www.readbyqxmd.com/read/29068450/soy-peptide-aggregates-formed-during-hydrolysis-reduced-protein-extraction-without-decreasing-their-nutritional-value
#20
Yuanhong Zhang, Feibai Zhou, Mouming Zhao, Zhengxiang Ning, Dongxiao Sun-Waterhouse, Baoguo Sun
Upon enzymatic hydrolysis, soy protein isolates showed a strong tendency to aggregate, presenting a significant loss of valuable proteins. This study mainly focused on the large insoluble aggregates formed during proteolysis, and the influence of heating was further explored for a better understanding of the mechanism involved. The results from SDS-PAGE and amino acid analysis clearly showed that the insoluble aggregates formed upon hydrolysis were aggregated peptides, mainly attributed to the hydrophobic interactions between peptides with hydrophobic amino acids (Val, Ala, Leu, Ile, Tyr, Phe, and Pro) and sulfur-containing (Met and Cys) residues...
October 25, 2017: Food & Function
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