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https://www.readbyqxmd.com/read/27883045/acidic-mammalian-chitinase-is-a-proteases-resistant-glycosidase-in-mouse-digestive-system
#1
Misa Ohno, Masahiro Kimura, Haruko Miyazaki, Kazuaki Okawa, Riho Onuki, Chiyuki Nemoto, Eri Tabata, Satoshi Wakita, Akinori Kashimura, Masayoshi Sakaguchi, Yasusato Sugahara, Nobuyuki Nukina, Peter O Bauer, Fumitaka Oyama
Chitinases are enzymes that hydrolyze chitin, a polymer of β-1, 4-linked N-acetyl-D-glucosamine (GlcNAc). Chitin has long been considered as a source of dietary fiber that is not digested in the mammalian digestive system. Here, we provide evidence that acidic mammalian chitinase (AMCase) can function as a major digestive enzyme that constitutively degrades chitin substrates and produces (GlcNAc)2 fragments in the mouse gastrointestinal environment. AMCase was resistant to endogenous pepsin C digestion and remained active in the mouse stomach extract at pH 2...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27882566/angiotensin-converting-enzyme-inhibitory-and-antioxidant-peptides-from-digestion-of-larvae-and-pupae-of-asian-weaver-ant-oecophylla-smaragdina-fabucious
#2
Werawich Pattarayingsakul, Arjaree Nilavongse, Onrapak Reamtong, Pamorn Chittavanich, Idsada Mungsantisuk, Yuvadee Mathong, Wipoo Prasitwuttisak, Watanalai Panbangred
BACKGROUND: Mixed larvae and pupae of weaver ant (Oecophylla smaragdina) are widely used as an important food ingredient in regions of Thailand. They have high nutritional values and comprise 53% protein and 13% lipid. Peptides derived from food proteins have been shown to possess biological activities. RESULTS: Peptides derived from pepsin and trypsin digestion of these weaver ant larvae and pupae were purified based on ACE inhibitory and antioxidant activities, and their amino acid sequences identified by LC-MS/MS...
November 23, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27876148/fc-specific-biotinylation-of-antibody-using-an-engineered-photoactivatable-z-biotin-and-its-biosensing-application
#3
Hong-Ming Yang, Ru-Meng Bao, Chang-Mei Yu, Yan-Na Lv, Wei-Fen Zhang, Jin-Bao Tang
The development of a site-specific and covalent attachment methodology is crucial for antibody-biotin conjugates to preserve the antigen-binding ability of antibodies and yield homogeneous products. In this study, an engineered photoactivatable Z-domain variant [an UV-active amino acid benzoylphenylalanine (Bpa) was genetically incorporated into the Z-domain] carrying one biotin molecule (ZBpa-Biotin) was prepared by employing aminoacyl-tRNA synthetase/suppressor tRNA and Avitag/BirA techniques. The site-specific and covalent attachment of IgG-biotin conjugates, viz...
January 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27867770/soymilk-residue-okara-as-a-natural-immobilization-carrier-for-lactobacillus-plantarum-cells-enhances-soymilk-fermentation-glucosidic-isoflavone-bioconversion-and-cell-survival-under-simulated-gastric-and-intestinal-conditions
#4
Xia Xiudong, Wang Ying, Liu Xiaoli, Li Ying, Zhou Jianzhong
Cell immobilization is an alternative to microencapsulation for the maintenance of cells in a liquid medium. However, artificial immobilization carriers are expensive and pose a high safety risk. Okara, a food-grade byproduct from soymilk production, is rich in prebiotics. Lactobacilli could provide health enhancing effects to the host. This study aimed to evaluate the potential of okara as a natural immobilizer for L. plantarum 70810 cells. The study also aimed to evaluate the effects of okara-immobilized L...
2016: PeerJ
https://www.readbyqxmd.com/read/27864476/anti-hinge-antibodies-recognize-igg-subclass-and-protease-restricted-neoepitopes
#5
Willem J J Falkenburg, Dirkjan van Schaardenburg, Pleuni Ooijevaar-de Heer, Michel W P Tsang-A-Sjoe, Irene E M Bultink, Alexandre E Voskuyl, Arthur E H Bentlage, Gestur Vidarsson, Gertjan Wolbink, Theo Rispens
Anti-hinge Abs (AHAs) target neoepitopes exposed after proteolytic cleavage of IgG. In this study, we explored the diversity of protease- and IgG subclass-restricted AHAs and their potential as immunological markers in healthy donors (HDs) and patients with rheumatoid arthritis (RA) or systemic lupus erythematosus (SLE). AHA reactivity against IgG-degrading enzyme of Streptococcus pyogenes (IdeS)- or pepsin-generated F(ab')2 fragments of all four human IgG subclasses was determined. AHA reactivity against one or more out of eight F(ab')2 targets was found in 68% (68 of 100) of HDs, 69% (68 of 99) of SLE patients, and 81% (79 of 97) of RA patients...
November 18, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27857543/isolation-and-characterization-of-pepsin-soluble-collagens-from-bones-skins-and-tendons-in-duck-feet
#6
Hyun-Wook Kim, In-Jun Yeo, Ko-Eun Hwang, Dong-Heon Song, Yong-Jae Kim, Youn-Kyung Ham, Tae-Jun Jeong, Yun-Sang Choi, Cheon-Jei Kim
The objectives of this study were conducted to characterize pepsin-soluble collagen (PSC) extracted from bones (PSC-B), skins (PSC-S), and tendons (PSC-T) of duck feet and to determine their thermal and structural properties, for better practical application of each part of duck feet as a novel source for collagen. PSC was extracted from each part of duck feet by using 0.5 M acetic acid containing 5% (w/w) pepsin. Electrophoretic patterns showed that the ratio between α1 and α2 chains, which are subunit polypeptides forming collagen triple helix, was approximately 1:1 in all PSCs of duck feet...
October 31, 2016: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/27855907/effects-of-temperature-ph-and-sugar-binding-on-the-structures-of-lectins-ebulin-f-and-selfd
#7
Celia Carrillo, Damián Cordoba-Diaz, Manuel Cordoba-Diaz, Tomás Girbés, Pilar Jiménez
Ebulin f and SELfd are two lectins of Sambucus ebulus L. that show different stability and digestibility properties in gastric fluid due to their structural differences which may explain their different toxicological profiles. The main aim was to determine the effects of pH, temperature and sugar binding on the intrinsic structures of both proteins by fluorescence analyses. Quenching experiments were conducted, under different pH and temperature conditions, with acrylamide (uncharged) and iodide (charged), to study the possible changes of their intrinsic fluorescence...
April 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27855881/designing-dairy-desserts-for-weight-management-structure-physical-properties-and-in-vitro-gastric-digestion
#8
Jennifer Borreani, Empar Llorca, Amparo Quiles, Isabel Hernando
The first aim of this study was to observe the effect of adding dairy proteins and reducing the cream content in order to obtain healthier dairy desserts for use in weight management. The extra-whey protein low-cream sample had the densest, firmest matrix, which is related to increased satiety. The second aim was to investigate the in vitro gastric digestion behavior of whey and casein proteins in a heat-treated semisolid real food. The extra-casein protein sample matrix broke down more slowly than the others because the caseins clotted at the gastric pH...
April 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27854344/unfolding-refolding-study-on-collagen-from-sea-cucumber-based-on-2d-fourier-transform-infrared-spectroscopy
#9
Lei Qin, Jing-Ran Bi, Dong-Mei Li, Meng Dong, Zi-Yuan Zhao, Xiu-Ping Dong, Da-Yong Zhou, Bei-Wei Zhu
We aimed to explore the differences of thermal behaviors between insoluble collagen fibrils (ICFs) and pepsin-solubilized collagens (PSCs) from sea cucumber Stichopus japonicus. The unfolding/refolding sequences of secondary structures of ICFs and PSCs during the heating and cooling cycle (5 → 70 → 5 °C) were identified by Fourier transform infrared spectrometry combined with curve-fitting and 2D correlation techniques. ICFs showed a higher proportion of α-helical structures and higher thermostability than PSCs, and thus had more-stable triple helical structures...
November 16, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27847428/effect-of-boiling-on-the-antidiabetic-property-of-enzyme-treated-sheep-milk-casein
#10
Farhath Jan, Santosh Kumar, Richa Jha
AIM: Sheep milk-born bioactive peptides have been found to exhibit various physiological activities. The present work was designed with the aim to evaluate the effect of boiling on antidiabetic property of peptides derived from sheep milk caseinate on hydrolysis with three different proteases. MATERIALS AND METHODS: In this investigation, casein prepared from raw and boiled sheep milk was hydrolyzed by three commercially available proteases (trypsin, pepsin, and chymotrypsin)...
October 2016: Veterinary World
https://www.readbyqxmd.com/read/27846786/systematic-comparison-of-protocols-for-the-preparation-of-human-articular-cartilage-for-use-as-scaffold-material-in-cartilage-tissue-engineering
#11
Cornelia Schneider, Johannes Lehmann, Gerjo van Osch, Florian Hildner, Andreas Herbert Teuschl, Xavier Monforte, David Miosga, Patrick Heimel, Eleni Priglinger, Heinz Redl, Susanne Wolbank, Sylvia Nürnberger
Natural extracellular matrix (ECM) derived biomaterials from decellularized allogenic tissues are of increasing interest for tissue engineering since their structure and composition provide a complexity that is not achievable with current manufacturing techniques. The prerequisite to bring allogenic tissue from bench to bedside as a functional biomaterial is the full removal of cells while preserving most of its native characteristics such as structure and composition. The exceptionally dense structure of articular cartilage, however, poses a special challenge for decellularisation, scaffold preparation and reseeding...
November 15, 2016: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27845023/antimicrobial-activity-of-buttermilk-and-lactoferrin-peptide-extracts-on-poultry-pathogens
#12
Catherine Jean, Martine Boulianne, Michel Britten, Gilles Robitaille
Antibiotics are commonly used in poultry feed as growth promoters. This practice is questioned given the arising importance of antibiotic resistance. Antimicrobial peptides can be used as food additives for a potent alternative to synthetic or semi-synthetic antibiotics. The objective of this study was to develop a peptide production method based on membrane adsorption chromatography in order to produce extracts with antimicrobial activity against avian pathogens (Salmonella enterica var. Enteritidis, Salmonella enterica var...
November 2016: Journal of Dairy Research
https://www.readbyqxmd.com/read/27842869/isolation-and-biochemical-characterisation-of-a-bacteriocin-like-substance-produced-by-bacillus-amyloliquefaciens-an6
#13
Hanen Ben Ayed, Hana Maalej, Noomen Hmidet, Moncef Nasri
This study focuses on the isolation and characterisation of a peptide with bacteriocin-like properties from Bacillus amyloliquefaciens An6. Incubation conditions were optimised, and the effects of the incubation period and of carbon and nitrogen sources were investigated. The produced bacteriocin was partially purified with ammonium sulphate precipitation, dialysis and ultrafiltration and was then biochemically characterised. Maximum bacteriocin production was achieved after 48h of incubation in a culture medium containing 20g/L starch and 10g/L yeast extract, with an initial pH 8...
December 2015: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27836720/effect-of-dietary-poly-%C3%AE-hydroxybutyrate-phb-on-growth-performance-intestinal-health-status-and-body-composition-of-pacific-white-shrimp-litopenaeus-vannamei-boone-1931
#14
Yafei Duan, Yue Zhang, Hongbiao Dong, Xiaoting Zheng, Yun Wang, Hua Li, Qingsong Liu, Jiasong Zhang
In the present study, the effect of dietary supplementation of poly-β-hydroxybutyrate (PHB) on the growth performance, intestinal digestive and immune function, intestinal short-chain fatty acids (SCFA) content and body composition of Pacific white shrimp Litopenaeus vannamei (Boone, 1931) was evaluated. The shrimp was fed for 35 days with four different diets: 0%, 1%, 3% and 5% PHB supplemented feed. The results indicated that supplementation of PHB significantly increased the growth performance of the shrimp, and the feed conversion rate (FCR) in 3%PHB treatment group was significantly lower than the control (P < 0...
November 8, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27832513/collagen-hydrolysates-of-skin-shavings-prepared-by-enzymatic-hydrolysis-as-a-natural-flocculant-and-their-flocculating-property
#15
Ruijie Fu, Kai Yao, Qisheng Zhang, Dongying Jia, Jiayuan Zhao, Yuanlong Chi
A series of collagen hydrolysates (CHs) were prepared from pigskin shavings by using pepsin (PCH), trypsin (TCH), Alcalase (ACH), HCl (HCH), and NaOH (NCH). Their physicochemical properties, including degree of collagen hydrolysis, molecular weight distribution, electric charge, and microstructure, were investigated, and their flocculation performance was evaluated in a kaolin suspension, at varied pHs and concentrations. PCH exhibited high flocculation capability under acidic and neutral conditions, and its efficiency for removing suspended particles was approximately 80% at a concentration of 0...
November 10, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27828767/comparative-risk-of-liver-and-intestinal-fluke-infection-from-either-wild-caught-or-cultured-fish-in-vietnam
#16
Thi Van Phan, Ngoc Thanh Bui, Van Ha Nguyen, Darwin Murrell
A survey for fish-borne zoonotic trematode (FZT) metacercariae in fish from the Northern Mountain Region of Vietnam was conducted from May to August 2014. The major objective was to evaluate the impact of water body types on the prevalence of FZT. A total of 846 fish (31 species) were collected from three water body types, including aquaculture ponds, rivers, and reservoirs. A pepsin digestion method was used for the recovery of metacercariae from fish. Overall, 29 of the 31 fish species were infected with FZTs...
December 2016: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/27812926/purification-and-characterization-of-a-novel-anti-campylobacter-bacteriocin-produced-by-lactobacillus-curvatus-dn317
#17
Mohamed Zommiti, Hamdan Almohammed, Mounir Ferchichi
The lactic acid bacteria (LAB) microbiota of Saudi chicken ceca was determined. From 60 samples, 204 isolates of lactic acid bacteria were obtained. Three isolates produced antimicrobial activities against Campylobacter jejuni, Listeria monocytogenes, and Bacillus subtilis. The isolate DN317, which had the highest activity against Campylobacter jejuni ATCC 33560, was identified as Lactobacillus curvatus (GenBank accession numbers: KX353849 and KX353850). Full inhibitory activity was observed after a 2-h incubation with the supernatant at pH values between 4 and 8...
November 3, 2016: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/27804059/collagen-extract-from-marine-finfish-scales-as-a-potential-mosquito-larvicide
#18
K Muthumari, M Anand, M Maruthupandy
Collagen is a peptide being utilized in medical, health care, nutrient and decorative industry. Marine fish scales are one of the good sources of collagen, which is extracted using the advanced enzymatic digestion method. Scales of Sardinella longiceps (Oil Sardine) have a high proportion of collagen. This product is well absorbed with broad adaptive values that encourage the inclusion of nutriments. In this paper, we have performed the isolation and characterization of collagen from S. longiceps fish scales...
December 2016: Protein Journal
https://www.readbyqxmd.com/read/27800131/determination-of-acquired-resistance-profiles-of-pseudomonas-aeruginosa-isolates-and-characterization-of-an-effective-bacteriocin-like-inhibitory-substance-blis-against-these-isolates
#19
Dariush Shokri, Mohammad Rabbani Khorasgani, Saeideh Zaghian, Seyed Masih Fatemi, Milad Mohkam, Younes Ghasemi, Asghar Taheri-Kafrani
BACKGROUND: The emergence of pan-drug resistant strains (PDR) of Pseudomonas aeruginosa has led to renewed efforts to identify alternative agents, such as bacteriocins and bacteriocin-like inhibitory substances (BLISs). OBJECTIVES: The aims of this study were to determine the acquired resistance profiles of multidrug-resistant (MDR), extensively drug-resistant (XDR), and PDR P. aeruginosa isolates based on the revised definitions of the CDC and ECDC and to screen and characterize effective BLISs against these isolates...
August 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27795886/biological-potency-and-characterization-of-antibacterial-substances-produced-by-lactobacillus-pentosus-isolated-from-hentak-a-fermented-fish-product-of-north-east-india
#20
Chirom Aarti, Ameer Khusro, Mariadhas Valan Arasu, Paul Agastian, Naïf Abdullah Al-Dhabi
Lactic acid bacteria (LAB) isolated from various foods are important due to their potential to inhibit microorganisms, including drug-resistant bacteria. The objectives of this investigation were to isolate and identify antibacterial substances producing LAB from Hentak, a traditional fermented fish product of Manipur (North-East India), and to optimize the production of antagonistic substances present in cell free neutralized supernatant (CFNS) against enteric bacterial pathogens using the 'one factor at a time' (OFAT) method...
2016: SpringerPlus
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