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https://www.readbyqxmd.com/read/29908505/a-green-soundscape-index-gsi-the-potential-of-assessing-the-perceived-balance-between-natural-sound-and-traffic-noise
#1
Pablo Kogan, Jorge P Arenas, Fernando Bermejo, María Hinalaf, Bruno Turra
Urban soundscapes are dynamic and complex multivariable environmental systems. Soundscapes can be organized into three main entities containing the multiple variables: Experienced Environment (EE), Acoustic Environment (AE), and Extra-Acoustic Environment (XE). This work applies a multidimensional and synchronic data-collecting methodology at eight urban environments in the city of Córdoba, Argentina. The EE was assessed by means of surveys, the AE by acoustic measurements and audio recordings, and the XE by photos, video, and complementary sources...
June 13, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29908272/a-novel-solvent-tolerant-esterase-of-gdsgg-motif-subfamily-from-solar-saltern-through-metagenomic-approach-recombinant-expression-and-characterization
#2
G Jayanath, Sowmya P Mohandas, Bhavya Kachiprath, Solly Solomon, T P Sajeevan, I S Bright Singh, Rosamma Philip
A novel esterase, designated as EstSP was identified by function based screening from a soil metagenomic fosmid library of solar saltern of Goa. EstSP gene of 1065 bp encoding a putative esterase of 354 amino acids showing 55% identity to esterase from gamma proteobacterium HIMB55 was identified. The enzyme EstSP belongs to family IV hormone sensitive lipase with novel sequence characteristics and a unique motif GDSGG. EstSP was expressed in Escherichia coli and purified protein with molecular mass of 58...
June 13, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29908270/complete-mitochondrial-genome-of-parasesarma-affine-brachyura-sesarmidae-gene-rearrangements-in-sesarmidae-and-phylogenetic-analysis-of-the-brachyura
#3
Zhengfei Wang, Ziqian Wang, Xuejia Shi, Qiong Wu, Yitao Tao, Huayun Guo, Chenyao Ji, Yuze Bai
In this study, the complete mitochondrial DNA (mtDNA) sequence of the crab Parasesarma affine is determined, characterized and compared with other decapod crustaceans. The P. affine mitochondrial genome (mitogenome) is 15,638 bp in size, and contains 13 protein-coding genes (PCGs), 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes and a control region (CR). Then, 23 of the 37 genes are encoded by the heavy (+) strand while 14 are encoded by the light (-) strand. All PCGs are initiated by ATN codons and 4 of the 13 PCGs harbored the incomplete termination codon by T or TA...
June 13, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29908246/refractory-ceramic-fibers-fiber-characteristics-potential-health-effects-and-clinical-observations
#4
Mark J Utell, L Daniel Maxim
Refractory ceramic fibers (RCFs) are amorphous fibers that belong to a class of materials termed synthetic vitreous fibers (SVFs), also called man-made mineral fibers (MMMFs), which includes alkaline earth silicate wool, glass wool, rock (stone) wool, slag wool, and special-purpose glass fibers. RCFs are more durable and biopersistent than several other SVFs, although very much less biopersistent than either amosite or crocidolite asbestos. Chronic inhalation studies indicated that rats and hamsters exposed to RCF fibers developed fibrosis and tumors...
June 13, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29908185/design-and-development-of-pegylated-liposomal-formulation-of-her2-blocker-lapatinib-for-enhanced-anticancer-activity-and-diminshed-cardiotoxicity
#5
Richa Shrivastava, Shruti Trivedi, Pankaj Kumar Singh, Mohammad Asif, Manish Kumar Chourasia, Amit Khanna, Smrati Bhadauria
Breast cancer is most frequently diagnosed cancer and fifth leading cause of death in women. About 20-30% of all breast cancers overexpress HER2/neu receptors. Lapatinib is a dual tyrosin kinase inhibitor of EGFR and HER2. It exhibits its anticancer effect via blocking intracellular domain of HER2 receptor in breast cancer. Lapatinib belongs to class II of BSC classification due to its poor solubility restricting its clinical application. Due to presence of HER2 receptor on cardiomyocytes, it is associated with generation of cardiotoxicity...
June 13, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29908169/a-modified-pcr-rflp-method-to-determine-genetic-diversity-of-giardia-lamblia-human-isolates-based-on-triosephosphate-isomerase-tpi-gene
#6
Ferial Rahimian, Javid Sadraei, Majid Pirestani, Fatemeh Ghaffarifar
An infection of digestive system, Giardiasis, caused by tiny parasites called Giardia lamblia (also known Giardia intestinalis or Giardia duodenalis). Giardia sp. is the most common intestinal parasite of humans and other animals throughout the world. Isolates of G. lamblia are classified into eight assemblages based on isoenzyme and DNA analyses. Assemblages A and B infect humans and a broad range of other hosts. The purpose of this study was to genotype human isolates of G. lamblia by PCR-RFLP in Karaj city...
June 13, 2018: Acta Tropica
https://www.readbyqxmd.com/read/29908130/designed-macrocyclic-peptides-as-nanomolar-amyloid-inhibitors-based-on-minimal-recognition-elements
#7
Anna Spanopoulou, Luzia Heidrich, Hong-Ru Chen, Christina Frost, Dean Hrle, Eleni Malideli, Kathleen Hille, Alexandros Grammatikopoulos, Jürgen Bernhagen, Martin Zacharias, Gerhard Rammes, Aphrodite Kapurniotu
Amyloid self-assembly is linked to the pathogenesis of Alzheimer's disease (AD) and type 2 diabetes (T2D), but so far none of the anti-amyloid molecules has reached the clinic. Macrocyclic peptides belong to the most attractive drug candidates. Here we present macrocyclic peptides (MCIPs) designed using minimal IAPP-derived recognition elements as a novel class of nanomolar amyloid inhibitors of both Abeta40(42) and IAPP or Abeta40(42) alone and show that chirality controls inhibitor selectivity. Sequence optimization led to the discovery of an Abeta40(42)-selective MCIP exhibiting high proteolytic stability in human plasma and human blood-brain-barrier (BBB) crossing ability in a cell model, two highly desirable properties for anti-amyloid drugs in AD...
June 16, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29907938/analysis-of-the-gut-microbial-diversity-of-dairy-cows-during-peak-lactation-by-pacbio-single-molecule-real-time-smrt-sequencing
#8
Weicheng Li, Qiangchuan Hou, Yanjie Wang, Huimin Ma, Yahua Liu, Feiyan Zhao, Jing Li, Lai-Yu Kwok, Jie Yu, Zhihong Sun, Tiansong Sun
BACKGROUND: The gut microbes of dairy cows are strongly associated with their health, but the relationship between milk production and the intestinal microbiota has seldom been studied. Thus, we explored the diversity of the intestinal microbiota during peak lactation of dairy cows. METHODS: The intestinal microbiota of nine dairy cows at peak lactation was evaluated using the Pacific Biosciences single-molecule real-time (PacBio SMRT) sequencing approach. RESULTS: A total of 32,670 high-quality 16S rRNA gene sequences were obtained, belonging to 12 phyla, 59 families, 107 genera, and 162 species...
June 15, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29907816/fish-mitigate-trophic-depletion-in-marine-cave-ecosystems
#9
Simona Bussotti, Antonio Di Franco, Carlo Nike Bianchi, Pierre Chevaldonné, Lea Egea, Emanuela Fanelli, Christophe Lejeusne, Luigi Musco, Carlos Navarro-Barranco, Alexis Pey, Serge Planes, Jean Vincent Vieux-Ingrassia, Paolo Guidetti
Dark marine habitats are often characterized by a food-limited condition. Peculiar dark habitats include marine caves, characterized by the absence of light and limited water flow, which lead to reduced fluxes of organic matter for cave-dwelling organisms. We investigated whether the most abundant and common cave-dwelling fish Apogon imberbis has the potential to play the role of trophic vector in Mediterranean marine caves. We first analysed stomach contents to check whether repletion changes according to a nycthemeral cycle...
June 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29907776/tobacco-smoking-related-prevalence-of-methanogens-in-the-oral-fluid-microbiota
#10
Ghiles Grine, Elodie Terrer, Mahmoud Abdelwadoud Boualam, Gérard Aboudharam, Hervé Chaudet, Raymond Ruimy, Michel Drancourt
The oral fluid microbiome comprises an important bacterial diversity, yet the presence of archaea has not been reported so far. In order to quest for the presence of methanogenic archaea (methanogens) in oral fluid, we used a polyphasic approach including PCR-sequencing detection, microscopic observation by fluorescence in-situ hybridization, isolation and culture, molecular identification and genotyping of methanogens in 200 oral fluid specimens. In the presence of negative controls, 64/200 (32%) prospectively analysed oral fluid specimens were PCR-positive for methanogens, all identified as Methanobrevibacter oralis by sequencing...
June 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29907537/first-report-on-giardia-duodenalis-assemblage-f-in-slovakian-children-living-in-poor-environmental-conditions
#11
Jana Pipiková, Ingrid Papajová, Viktória Majláthová, Jindřich Šoltys, Júlia Bystrianska, Ingrid Schusterová, Veronika Vargová
BACKGROUND: Giardiasis is one of the most common gastrointestinal infections of humans and animals attributable to complex of eight morphologically identical genetic assemblages, further divided into sub-assemblages. Disease is common for a wide range of hosts and genetic characterization is needed for better understanding of multifaceted epidemiology for this protozoan parasite. The aim of this study was to identify genetic heterogeneity in assemblages and sub-assemblages of Giardiaduodenalis circulating among the children population living in deprived socioeconomic conditions...
June 2, 2018: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/29907496/-where-do-we-stand-with-benign-prostatic-hyperplasia-day-case-surgery-a-laser-effect
#12
L Gury, G Robert, H Bensadoun
INTRODUCTION AND OBJECTIVES: Despite its feasibility has been proven, Benign Prostatic Hyperplasia (BPH) day-case surgery remains uncommon. Our objective was to describe the evolution of BPH day-case surgery in France according to the surgical technique employed. MATERIALS AND METHODS: We extracted data from the Information System of Medicalization Program (PMSI) including all of the hospital stays in France from 2010 to 2016. Patients belonging to the transurethral prostatectomy homogeneous group of patients (GHM 12C04) and having as a main diagnosis prostatic hyperplasia (N40) or benign prostatic tumor (D291) were included in the analysis...
June 12, 2018: Progrès en Urologie
https://www.readbyqxmd.com/read/29907390/clinical-characteristics-of-citrobacter-meningitis-in-adults-high-incidence-in-patients-with-a-postneurosurgical-state-and-strains-not-susceptible-to-third-generation-cephalosporins
#13
Chia-Yi Lien, Jun-Jun Lee, Chun-Chih Chien, Chi-Ren Huang, Cheng-Hsien Lu, Wen-Neng Chang
Adult bacterial meningitis (ABM) caused by Citrobacter (C.) infection is very uncommon and the clinical characteristics of this specific infectious syndrome have not been analyzed in the literature. The clinical characteristics of six Citrobacter ABM patients collected during a study period of 30 years (1986-2015) were enrolled, and they accounted for 1.1% (6/540) of our ABM patients. In this study, a total of 14 patients with Citrobacter ABM (six collected from our hospital and eight from the literature) were included for analysis...
June 12, 2018: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/29907302/novel-transcription-factors-pvbmy1-and-pvbmy3-increase-biomass-yield-in-greenhouse-grown-switchgrass-panicum-virgatum-l
#14
Madana M R Ambavaram, Aminat Ali, Kieran P Ryan, Oliver Peoples, Kristi D Snell, Maria N Somleva
Increasing crop yield requires the coordination of multiple metabolic pathways spanning photosynthetic carbon fixation, central carbon metabolism, and finally targeted carbon deposition to end product. In this study, we used a transcriptome-based gene regulatory association network to search for transcription factor genes that could play a role in increasing carbon flow through pathways associated with these processes to increase biomass yield in switchgrass. Two novel switchgrass transcription factors, PvBMY1 (BioMass Yield 1, belonging to the APETALA2/Ethylene Response Factor family of transcription factors) and PvBMY3 (BioMass Yield 3, a member of the Nuclear-Factor Y family of transcription factors), with predicted roles in the regulation of photosynthesis and related metabolism were identified...
August 2018: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29907282/study-of-a-complex-secondary-metabolites-with-potent-anti-radical-activity-by-two-dimensional-tlc-hplc-coupled-to-electrospray-ionization-time-of-flight-mass-spectrometry-and-bioautography
#15
Krzysztof Kamil Wojtanowski, Tomasz Mroczek
The high performance liquid chromatography (HPLC) coupled to electrospray ionization (ESI) octopole orthogonal acceleration time-of-flight (TOF) mass spectrometry (MS) was developed to separate secondary metabolites belonging to different groups of compounds with a wide range of polarity, i.e. phenolic acids, flavonoids, sesquiterpenes and others present in minute amounts, in a single run. Moreover, application of ESI-TOF-MS with a simple in source type of fragmentation and high mass accuracy measurements (around 2 ppm) in both positive and negative ionization modes with different and optimized fragmentor voltages, enabled the identification of each single compound based on proposed fragmentation patterns...
October 31, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29907122/detection-of-bacterial-pathogens-in-clade-e-head-lice-collected-from-niger-s-refugees-in-algeria
#16
Meriem Louni, Nadia Amanzougaghene, Nassima Mana, Florence Fenollar, Didier Raoult, Idir Bitam, Oleg Mediannikov
BACKGROUND: Head lice, Pediculus humanus capitis, are obligate blood-sucking parasites. Phylogenetically, they occur in five divergent mitochondrial clades (A, D, B, C and E), each having a particular geographical distribution. Recent studies have revealed that head lice, as is the case of body lice, can act as a vector for louse-borne diseases. Here, we aimed to study the genetic diversity of head lice collected from Niger's refugees (migrant population) arriving in Algeria, northern Africa, and to look for louse-borne pathogens...
June 15, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29907093/effect-of-administration-of-a-probiotic-preparation-on-gut-microbiota-and-immune-response-in-healthy-women-in-india-an-open-label-single-arm-pilot-study
#17
Ankita Singh, Aditya N Sarangi, Amit Goel, Rajni Srivastava, Rajat Bhargava, Priyanka Gaur, Amita Aggarwal, Rakesh Aggarwal
BACKGROUND: Probiotics have been shown to be useful for the treatment of many disease conditions. These beneficial effects are believed to be mediated by change in the composition of gut microbiota and modulation of the host immune responses. However, the available data on the effect of probiotics on these parameters are quite limited. METHODS: We studied the composition of fecal microbiota, using 16S rRNA sequencing, and host immune responses in peripheral blood (plasma cytokine levels, T cell subsets and in vitro cytokine production after stimulation with anti-CD3/CD28 antibody or lipopolysaccharide) in a group of 14 healthy women at three time-points - before and after administration of a probiotic preparation (a capsule of VSL#3, each containing 112...
June 15, 2018: BMC Gastroenterology
https://www.readbyqxmd.com/read/29907090/fecal-carriage-of-multidrug-resistant-escherichia-coli-by-community-children-in-southern-taiwan
#18
I-Fei Huang, Wei-Yang Lee, Jiun-Ling Wang, Chih-Hsin Hung, Hong-Hsiang Hu, Wan-Yu Hung, Yun-Ju Hung, Wen-Chi Chen, Ying-Tso Shen, Ming-Fang Cheng
BACKGROUND: The emergence of multidrug-resistant (MDR) Escherichia coli (E. coli), particularly E. coli sequence type ST131, is becoming a global concern. Commensal bacteria, an important reservoir of antibiotic resistance genes, facilitate the spread of such genes to pathogenic bacterial strains. The objective of the study is to investigate the fecal carriage of MDR E. coli and ST131 E. coli in community children in Southern Taiwan. METHODS: In this prospective study, stool samples from children aged 0-18 years were obtained within 3 days of hospitalization from October 2013 to September 2014...
June 15, 2018: BMC Gastroenterology
https://www.readbyqxmd.com/read/29906976/magnetic-quasi-long-range-ordering-in-nematic-systems-due-to-competition-between-higher-order-couplings
#19
Milan Žukovič, Georgii Kalagov
Critical properties of the two-dimensional XY model involving solely nematic-like terms of the second and third orders are investigated by spin-wave analysis and Monte Carlo simulation. It is found that, even though neither of the nematic-like terms alone can induce magnetic ordering, their coexistence and competition leads to an extended phase of the magnetic quasi-long-range-order phase, wedged between the two nematic-like phases induced by the respective couplings. Thus, except for the multicritical point, at which all the phases meet, for any finite value of the coupling parameters ratio there are two phase transition: one from the paramagnetic phase to one of the two nematic-like phases followed by another one at lower temperatures to the magnetic phase...
May 2018: Physical Review. E
https://www.readbyqxmd.com/read/29906959/product-sum-universality-and-rushbrooke-inequality-in-explosive-percolation
#20
M M H Sabbir, M K Hassan
We study explosive percolation (EP) on an Erdös-Rényi network for product rule (PR) and sum rule (SR). Initially, it was claimed that EP describes discontinuous phase transition; now it is well accepted as a probabilistic model for thermal continuous phase transition (CPT). However, no model for CPT is complete unless we know how to relate its observable quantities with those of thermal CPT. To this end, we define entropy and specific heat, redefine susceptibility, and show that they behave exactly like their thermal counterparts...
May 2018: Physical Review. E
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