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https://www.readbyqxmd.com/read/28330310/diversity-and-bioactive-potentials-of-culturable-heterotrophic-bacteria-from-the-surficial-sediments-of-the-arabian-sea
#1
Abdulaziz Anas, Charulatha Nilayangod, C Jasmin, Saradavey Vinothkumar, P S Parameswaran, Shanta Nair
Marine sediments accommodate plethora of diverse microorganisms with varying ecological functions. In the present study, we isolated bacteria from surficial sediments of south east Arabian Sea (AS) and evaluated their bioactive potentials. A total of 131 isolates belonging to the phylum: γ-Proteobacteria (63%), Bacillales (34%) and Micrococcaceae (3%) were isolated. Among these, about 40% of the isolates showed the presence of secondary metabolite biosynthetic genes such as PKS or NRPS or both. Organic extracts of nearly 50% of these organisms were cytotoxic to human breast cancer MCF-7 cells and were bactericidal to human pathogens, Escherichia coli and Pseudomonas sp...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330309/molecular-characterization-of-phytoplasmas-of-clover-proliferation-group-associated-with-three-ornamental-plant-species-in-india
#2
Ekta Khasa, Gopala, Aido Taloh, T Prabha, Madhupriya, G P Rao
Suspected phytoplasma symptoms of little leaf, yellowing, chlorosis, phyllody, witches' broom, and stunting were observed on ten different ornamental plant species at New Delhi, Andhra Pradesh, Haryana, Bengaluru, and Pune, India, during March to July 2016. To investigate the possibility of phytoplasma etiology, PCR assays were performed using universal primer pairs (P1/P7 followed by 3Far/3Rev) specific to the phytoplasma 16Sr RNA gene. First round PCR amplification with primer pair P1/P7 did not yield expected 1...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330299/detection-of-antimicrobial-traits-in-fluorescent-pseudomonads-and-molecular-characterization-of-an-antibiotic-pyoluteorin
#3
J U Vinay, M K Naik, R Rangeshwaran, G Chennappa, Sohel S Shaikh, R Z Sayyed
Thirty isolates of fluorescent pseudomonads were obtained from rhizosphere of different crops in Raichur, India. The fluorescent pseudomonad strains were characterized in vitro for biochemical traits, antimicrobial traits, and pyoluteorin antibiotic production. All the isolates that showed fluorescent pigment production under UV light were rod shaped, Gram negative, positive for oxidase, catalase and citrate utilization tests, and negative for indole test. Out of 30 isolates, 07 isolates were positive for HCN production, 15 isolates were positive for H2S production, and all the isolates were positive for siderophore production...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330280/culturable-endophytic-bacteria-associated-with-medicinal-plant-ferula-songorica-molecular-phylogeny-distribution-and-screening-for-industrially-important-traits
#4
Yong-Hong Liu, Jian-Wei Guo, Nimaichand Salam, Li Li, Yong-Guang Zhang, Jian Han, Osama Abdalla Mohamad, Wen-Jun Li
Xinjiang, a region of high salinity and drought, is a host to many arid and semi-arid plants. Many of these plants including Ferula spp. have indigenous pharmaceutical histories. As many of the medicinal properties of plants are in tandem with the associated microorganisms residing within the plant tissues, it is advisable to explore the endophytic potential of such plants. In the present study, diversity of culturable bacteria isolated from medicinal plants Ferula songorica collected from Hebukesaier, Xinjiang were analyzed...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330269/identification-of-the-traditional-and-non-traditional-sulfate-reducing-bacteria-associated-with-corroded-ship-hull
#5
Kiana Alasvand Zarasvand, V Ravishankar Rai
Pitting corrosion due to microbial activity is the most severe type of corrosion that occurs in ship hull. Since biogenic sulfide produced by sulfate-reducing bacteria (SRB) is involved in the acceleration of pitting corrosion of marine vessels, so it is important to collect information about SRB community involved in maritime vessel failure. We investigated the SRB community on corroded hull portion of the ship. With the use of common cultural method and 16S rDNA sequencing, ten bacteria with sulfate reduction ability were isolated and identified...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330254/enhanced-production-of-an-anti-malarial-compound-artesunate-by-hairy-root-cultures-and-phytochemical-analysis-of-artemisia-pallens-wall
#6
Zarna Pala, Vishnu Shukla, Anshu Alok, Subhash Kudale, Neetin Desai
Artemisinin and its derivatives are still one of the most effective drugs for the treatment of malaria. Artemisia pallens commonly known as Dhavanam, is an aromatic herb belonging to the family Asteraceae. Increasing the artemisinin content of A. pallens by genetic engineering would improve the availability of this much needed drug. In the present study, Agrobacterium rhizogenes (strain NCIM 5140) mediated genetic transformation of Artemisia pallens were carried out for hairy root induction. The effect of different media (Half MS, MS, MS along with BAP 0...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330244/elucidation-of-impact-of-heavy-metal-pollution-on-soil-bacterial-growth-and-extracellular-polymeric-substances-flexibility
#7
Muniswamy David, Paidi Murali Krishna, Jeybalan Sangeetha
Metal bioaccessibility is an alarming issue in croplands of mining sites due to overloading of toxic metals. Hence, the present study is aimed to determine the overloading of toxic metal in croplands across the Tawag village, Hutti, Raichur, India. Correspondingly, to identify the soil bacterial growth, physiological oxidative stress enzyme activity and surface macromolecular functional group evolution were analysed in and around the toxic metal contaminated sites through FT-IR and FT-Raman spectrometry. The evaluated results attribute that the study area is heavily polluted with the toxic metals such as arsenic, cadmium, chromium, lead and zinc...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330241/purification-characterization-gene-cloning-and-expression-of-gh-10-xylanase-penicillium-citrinum-isolate-hzn13
#8
Zabin K Bagewadi, Sikandar I Mulla, Harichandra Z Ninnekar
An extracellular thermostable xylanase (Xyl-IIb) produced by Penicillium citrinum isolate HZN13 was purified to homogeneity using DEAE-Sepharose, Sephadex G-100 and Bio-Gel P-60 chromatography with specific activity of 6272.7 U/mg and 19.6-fold purification. The purification revealed the occurrence of multiple forms of xylanases (Xyl-I, Xyl-IIa, Xyl-IIb and Xyl-III). The molecular mass of highly purified Xyl-IIb was ~31 kDa with SDS-PAGE. The enzyme was cellulase-free, thermostable (55-75 °C) and acidophilic (3...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330174/salicornia-evaluating-the-halophytic-extremophile-as-a-food-and-a-pharmaceutical-candidate
#9
REVIEW
Seema Patel
Food insecurity is a major issue in current scenario where a large section of mankind is at risk of insufficient diet. As food productivity has its limits, the prospecting of unutilized or underutilized flora as food candidates is collectively recognized as a sustainable option. In the past decade, a number of obscure plants have been identified to be rich in dietary components and deemed fit for integration into the food platter. This review discusses a candidate Salicornia, belonging to family Amaranthaceae...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330160/characterization-of-cla-producing-butyrivibrio-spp-reveals-strain-specific-variations
#10
S K Asraf Hussain, Anima Srivastava, Ashish Tyagi, Umesh Kumar Shandilya, Ashwani Kumar, Sachin Kumar, Surbhi Panwar, Amrish Kumar Tyagi
Conjugated Linoleic Acid (CLA), a fatty acid with high nutraceutical value is produced in rumen by resident bacterial species, especially Butyrivibrio spp. The present study was undertaken to examine the diversity of indigenous Butyrivibrio spp. from rumen liquor of Indian ruminants. The isolates were screened for their CLA production capability at different level of linoleic acid (LA) (0, 200, 400, 600, 800 μg/ml) at different time intervals (0, 2, 4, 6, 12, and 24 h). A total of more than 300 anaerobic cultures were isolated and 31 of them were identified as Butyrivibrio spp...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330150/phylogenetic-relationships-of-fluorescent-pseudomonads-deduced-from-the-sequence-analysis-of-16s-rrna-pseudomonas-specific-and-rpod-genes
#11
Asmita Rajwar, Manvika Sahgal
Phylogenetic relationship of 22 FLPs was revealed on the basis of polymorphism in three genes namely 16S rDNA, Pseudomonas-specific and rpoD gene regions. The primers for 16S rDNA, Pseudomonas-specific region and rpoD gene region were amplifying a region of 1492, 990 and 760 bp, respectively, from all the isolates investigated. The RFLP analysis of the PCR products resulted in a classification of these fluorescent pseudomonads which was best answered by rpoD-based RFLP analysis. The 22 FLPs were placed in two major clusters and seven subclusters suggesting that these were genotypically heterogenous and might belong to several species within Pseudomonas sensu stricto...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330142/an-overview-of-physico-chemical-mechanisms-of-biogas-production-by-microbial-communities-a-step-towards-sustainable-waste-management
#12
REVIEW
Ramansu Goswami, Pritam Chattopadhyay, Arunima Shome, Sambhu Nath Banerjee, Amit Kumar Chakraborty, Anil K Mathew, Shibani Chaudhury
Biogas is a combination of methane, CO2, nitrogen, H2S and traces of few other gases. Almost any organic waste can be biologically transformed into biogas and other energy-rich organic compounds through the process of anaerobic digestion (AD) and thus helping in sustainable waste management. Although microbes are involved in each step of AD, knowledge about those microbial consortia is limited due to the lack of phylogenetic and metabolic data of predominantly unculturable microorganisms. However, culture-independent methods like PCR-based ribotyping has been successfully employed to get information about the microbial consortia involved in AD...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330127/diversity-of-toxic-and-phytopathogenic-fusarium-species-occurring-on-cereals-grown-in-karnataka-state-india
#13
H Nagaraja, G Chennappa, K Poorna Chandra Rao, G Mahadev Prasad, M Y Sreenivasa
A total of 198 cereal samples (53 maize, 54 sorghum, 37 paddy and 54 wheat) were collected from 11 districts of Karnataka to understand the percent infection (PI), relative density (RD) and their frequency (Fr) caused by Fusarium spp. All samples were screened by agar plating method using MGA 2.5 agar media and incubated at 25 ± 2 °C for 3-5 days. The study revealed the association of 10 different Fusarium species known trichothecene producers such as Fusarium acuminatum, F. avenaceum, F. crookwellense, F...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330119/degradation-of-fipronil-by-stenotrophomonas-acidaminiphila-isolated-from-rhizospheric-soil-of-zea-mays
#14
Shivani Uniyal, Rashmi Paliwal, R K Sharma, J P N Rai
Fipronil is a widely used insecticide in agriculture and can cause potential health hazards to non-target soil invertebrates and nearby aquatic systems. In the present study, a fipronil degrading bacterium was isolated from fipronil contaminated soil, i.e. rhizospheric zone of Zea mays. Morphological, biochemical and molecular characterization of strain indicated that it clearly belongs to Stenotrophomonas acidaminiphila (accession no. KJ396942). A three-factor Box-Behnken experimental design combined with response surface modeling was employed to predict the optimum conditions for fipronil degradation...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330116/novel-pathway-of-cypermethrin-biodegradation-in-a-bacillus-sp-strain-sg2-isolated-from-cypermethrin-contaminated-agriculture-field
#15
Pankaj, Anita Sharma, Saurabh Gangola, Priyanka Khati, Govind Kumar, Anjana Srivastava
Pesticides belonging to pyrethroid group are widely used in agricultural fields to check pest infestation in different crops for enhanced food production. In spite of beneficial effects, non-judicious use of pesticides imposes harmful effect on human health as their residues reach different food materials and ground water via leaching, percolation and bioaccumulation. Looking into the potential of microbial degradation of toxic compounds under natural environment, a cypermethrin-degrading Bacillus sp. was isolated from pesticide-contaminated soil of a rice field of Distt...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330093/exploration-of-nifh-gene-through-soil-metagenomes-of-the-western-indian-himalayas
#16
Ravindra Soni, Deep Chandra Suyal, Santosh Sai, Reeta Goel
This group has previously highlighted the prevalence of Csp genes from cold Himalayan environments. However, this study has explored the uncultured diazotrophs from metagenomes of western Indian Himalayas. The metagenomic nifH gene clone library was constructed from the Temperate, Subtropical and Tarai soils of Western Himalaya, India followed by polymerase chain reaction (PCR) amplification. After preliminary screening, selected clones were sequenced. In silico analysis of the clones was done, which documented 83...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330085/bioprospecting-and-biotechnological-applications-of-fungal-laccase
#17
REVIEW
Pooja Upadhyay, Rahul Shrivastava, Pavan Kumar Agrawal
Laccase belongs to a small group of enzymes called the blue multicopper oxidases, having the potential ability of oxidation. It belongs to enzymes, which have innate properties of reactive radical production, but its utilization in many fields has been ignored because of its unavailability in the commercial field. There are diverse sources of laccase producing organisms like bacteria, fungi and plants. In fungi, laccase is present in Ascomycetes, Deuteromycetes, Basidiomycetes and is particularly abundant in many white-rot fungi that degrade lignin...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330077/diversity-and-antagonistic-potential-of-marine-microbes-collected-from-south-west-coast-of-india
#18
S Sinimol, A R Sarika, A Jayakumaran Nair
The diversity of some of the culturable microorganisms associated with marine flora and fauna collected off Vizhinjam and Mulloor coast of South India was evaluated and their bioactive production potential determined. From a total of 24 bacteria, 4 actinomycetes and 8 fungi isolated from diverse marine sources, five bacterial species-BLM3, BSP2, BCS1, BCS4 and BMA6 showed inhibitory activity against at least one of the tested pathogens viz., Klebsiella pneumonia KU1, Pseudomonas aeruginosa VL3, Salmonella enterica typhimurium MTCC 98, Escherichia coli MTCC 40, Micrococcus luteus MTCC 105, Staphylococcus simulans MTCC 3610, Proteus vulgaris MTCC 426, Vibrio fluvialis, Vibrio sp...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330012/mechanical-thrombectomy-for-minor-and-mild-stroke-patients-harboring-large-vessel-occlusion-in-the-anterior-circulation-a-multicenter-case-control-study
#19
Cyril Dargazanli, Caroline Arquizan, Arturo Consoli, Benjamin Gory, Omer Eker, Xavier Ayrignac, Jean-Pierre Decroix, Astrid Corlobé, Isabelle Mourand, Grégory Gascou, Mahmoud Charif, Julien Labreuche, Alain Duhamel, Paul-Emile Labeyrie, Hocine Redjem, Gabriele Ciccio, Stanislas Smajda, Carlos Riquelme, Oguzhan Coskun, Jean-Philippe Desilles, Frédéric Bourdain, Roberto Riva, Mikael Obadia, Francis Turjman, Georges Rodesch, Pierre Labauge, Alain Bonafé, Mikael Mazighi, Vincent Costalat, Michel Piotin, Raphaël Blanc, Bertrand Lapergue
BACKGROUND AND PURPOSE: Proximal large vessel occlusion (LVO) is present in up to 30% of minor strokes. There is no proven effectiveness of MT in the subgroup of minor stroke with LVO in the anterior circulation and data about mechanical thrombectomy (MT) in this subgroup of patients are sparse, with optimal management of these patients being yet not definitely addressed. The purpose of this case-control study was to evaluate MT in patients suffering from acute ischemic stroke (AIS) and LVO in the anterior circulation, presenting with minor to mild stroke symptoms (NIHSS<8)...
March 2017: Journal of Neuroradiology. Journal de Neuroradiologie
https://www.readbyqxmd.com/read/28329910/nucleosome-mobility-and-the-regulation-of-gene-expression-insights-from-single-molecule-studies
#20
REVIEW
Sergei Rudnizky, Omri Malik, Adaiah Bavly, Lilach Pnueli, Philippa Melamed, Ariel Kaplan
Nucleosomes at the promoters of genes regulate the accessibility of the transcription machinery to DNA, and function as a basic layer in the complex regulation of gene expression. Our understanding of the role of the nucleosome's spontaneous, thermally driven position changes in modulating expression is lacking. This is the result of the paucity of experimental data on these dynamics, at high-resolution, and for DNA sequences that belong to real, transcribed genes. We have developed an assay that uses partial, reversible unzipping of nucleosomes with optical tweezers to repeatedly probe a nucleosome's position over time...
March 22, 2017: Protein Science: a Publication of the Protein Society
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