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https://www.readbyqxmd.com/read/28449274/a-dna-barcode-library-for-germany-s-mayflies-stoneflies-and-caddisflies-ephemeroptera-plecoptera-trichoptera
#1
Jérôme Morinière, Lars Hendrich, Michael Balke, Arne J Beermann, Tobias König, Monika Hess, Stefan Koch, Reinhard Müller, Florian Leese, Paul D N Hebert, Axel Hausmann, Christoph F Schubart, Gerhard Haszprunar
Mayflies, stoneflies and caddisflies (Ephemeroptera, Plecoptera and Trichoptera) are prominent representatives of aquatic macroinvertebrates, commonly used as indicator organisms for water quality and ecosystem assessments. However, unambiguous morphological identification of EPT species, especially their immature life stages, is a challenging, yet fundamental task. However, a comprehensive DNA barcode library based upon taxonomically well-curated specimens can overcome the problematic identification. Once available, this library will support the implementation of fast, cost-efficient, and reliable DNA-based identifications and assessments of ecological status...
April 27, 2017: Molecular Ecology Resources
https://www.readbyqxmd.com/read/28448743/circular-rnas-with-hammerhead-ribozymes-encoded-in-eukaryotic-genomes-the-enemy-at-home
#2
Marcos de la Peña, Amelia Cervera
A new family of non-autonomous retrotransposons with self-cleaving hammerhead ribozymes, the so called retrozymes, has recently been found encoded in a number of plant genomes. These retroelements can be actively transcribed, and their RNAs accumulate in the cells as abundant non-coding circular RNAs (circRNAs) of small size (600-1000 nt). Related circRNAs with self-cleaving ribozymes had already been described in plants, and belong to a group of infectious RNA agents with an uncertain origin: the viroids and viroid-like satellites of plant RNA viruses...
April 27, 2017: RNA Biology
https://www.readbyqxmd.com/read/28448616/abundance-profiling-of-specific-gene-groups-using-precomputed-gut-metagenomes-yields-novel-biological-hypotheses
#3
Konstantin Yarygin, Alexander Tyakht, Andrey Larin, Elena Kostryukova, Sergei Kolchenko, Vilgelm Bitner, Dmitry Alexeev
The gut microbiota is essentially a multifunctional bioreactor within a human being. The exploration of its enormous metabolic potential provides insights into the mechanisms underlying microbial ecology and interactions with the host. The data obtained using "shotgun" metagenomics capture information about the whole spectrum of microbial functions. However, each new study presenting new sequencing data tends to extract only a little of the information concerning the metabolic potential and often omits specific functions...
2017: PloS One
https://www.readbyqxmd.com/read/28448583/characterization-of-the-fecal-microbiome-during-neonatal-and-early-pediatric-development-in-puppies
#4
Blake C Guard, Hanna Mila, Jörg M Steiner, Claire Mariani, Jan S Suchodolski, Sylvie Chastant-Maillard
Limited information is available describing the development of the neonatal fecal microbiome in dogs. Feces from puppies were collected at 2, 21, 42, and 56 days after birth. Feces were also collected from the puppies' mothers at a single time point within 24 hours after parturition. DNA was extracted from fecal samples and 454-pyrosequencing was used to profile 16S rRNA genes. Species richness continued to increase significantly from 2 days of age until 42 days of age in puppies. Furthermore, microbial communities clustered separately from each other at 2, 21, and 42 days of age...
2017: PloS One
https://www.readbyqxmd.com/read/28447504/placental-growth-factor-plgf-as-an-angiogenic-inflammatory-switcher-lesson-from-early-pregnancy-losses
#5
Hamid Reza Nejabati, Zeinab Latifi, Tohid Ghasemnejad, Amir Fattahi, Mohammad Nouri
Placental growth factor (PlGF) is an angiogenic factor which belongs to vascular endothelial growth factor (VEGF) family. In addition to the angiogenic function of PlGF, in some conditions such as preeclampsia and early pregnancy losses, it can induce inflammatory reactions which could be accompanied with reduced angiogenesis. Hence, it is crucial to investigate inflammatory and angiogenic switching states and understand underlying mechanisms. PlGF is expressed in endometrium, placenta and trophoblast cells and is involved in maturation of uterine NK cells...
April 27, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28447383/manganese-transport-is-essential-for-n2-fixation-by-rhizobium-leguminosarum-in-bacteroids-from-galegoid-but-not-phaseoloid-nodules
#6
Graham Hood, Vinoy Ramachandran, Alison East, J Allan Downie, Philip S Poole
Rhizobium leguminosarum has two high-affinity Mn(2+) transport systems encoded by sitABCD and mntH. In symbiosis, sitABCD and mntH were expressed throughout nodules and also strongly induced in Mn(2+) -limited cultures of free-living cells. Growth of a sitA mntH double mutant was severely reduced under Mn(2+) limitation and sitA and mntH single mutants were more sensitive to oxidative stress. The double sitA mntH mutant of R. leguminosarum was unable to fix nitrogen (Fix(-) ) with legumes belonging to the galegoid clade (Pisum sativum, Vicia faba and Vicia hirsuta)...
April 26, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28447266/in-depth-characterization-of-bacterial-and-archaeal-communities-present-in-the-abandoned-kettara-pyrrhotite-mine-tailings-morocco
#7
Odile Bruneel, N Mghazli, R Hakkou, I Dahmani, A Filali Maltouf, L Sbabou
In Morocco, pollution caused by closed mines continues to be a serious threat to the environment, like the generation of acid mine drainage. Mine drainage is produced by environmental and microbial oxidation of sulfur minerals originating from mine wastes. The fundamental role of microbial communities is well known, like implication of Fe-oxidizing and to a lesser extent S-oxidizing microorganism in bioleaching. However, the structure of the microbial communities varies a lot from one site to another, like diversity depends on many factors such as mineralogy, concentration of metals and metalloids or pH, etc...
April 26, 2017: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/28447161/prediction-of-graft-versus-host-disease-a-biomarker-panel-based-on-lymphocytes-and-cytokines
#8
Holger Budde, Susanne Papert, Jens-Holger Maas, Holger M Reichardt, Gerald Wulf, Justin Hasenkamp, Joachim Riggert, Tobias J Legler
Graft-versus-host disease (GvHD) still belongs to the major challenges after allogeneic hematopoietic stem cell transplantation (HSCT). Immune-suppressive therapy against GvHD is a double-edged sword due to risk of infections and relapse. The ability to adapt prophylactic treatment according to the probability of severe GvHD would be an essential advantage for the patients. We analyzed different biomarkers for their potential to predict the development of GvHD in 28 patients who underwent allogeneic HSCT. Blood was taken once directly after hematopoietic engraftment...
April 26, 2017: Annals of Hematology
https://www.readbyqxmd.com/read/28447046/genetic-diversity-analysis-of-morchella-sp-by-rapd
#9
Muhammad Irfan, Shuang Yang, Luo Yuxin, Jia-Xing Sun
This study investigated the genetic diversity of morchella species using randomly amplified polymorphic DNA markers. Morchella species are an important group of edible mushrooms belonging to the family Helveliaceae with medicinal and economical significance. In this study we have developed an efficient method of genomic DNA isolation which was amplified by eight RAPD primers to test the polymorphism in three species of morchella. Out of all eight primers tested in current study, one of them (B8) resulted 100% polymorphism among the three studied species...
March 2017: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28447044/molecular-and-phylogenetic-characterization-of-thelohanellus-boggoti-qadri-1962-cnidaria-myxosporea-bivalvulida-infecting-the-fin-of-indian-minor-carp-labeo-dero-hamilton-1822
#10
Harpreet Kaur, Aditya Gupta
A myxozoan parasite belonging to the genus Thelohanellus Kudo, 1933 (Cnidaria, Myxosporea, Bivalvulida) was isolated from the fins of Labeo dero inhabiting Ranjit Sagar Wetland, Punjab, India. The plasmodium was 0.5-0.7 mm in diameter each containing 80- 100 number of myxospores. Myxospores were egg shaped to ovoidal in valvular view having bluntly pointed anterior and broad rounded posterior end measuring 8.36x 4.77 µm. Polar capsule was single, broadly pyriform in shape, measuring 4.77x3.98 µm in size containing a polar filament coiled perpendicular to the longitudinal axis of myxospore body making 7-9 turns...
March 2017: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28446899/clonal-spread-of-16s-rrna-methyltransferase-producing-klebsiella-pneumoniae-st37-with-high-prevalence-of-esbls-from-companion-animals-in-china
#11
Jing Xia, Liang-Xing Fang, Ke Cheng, Guo-Hao Xu, Xi-Ran Wang, Xiao-Ping Liao, Ya-Hong Liu, Jian Sun
We screened 30 Klebsiella pneumoniae isolates from dogs and cats at a single animal hospital in Guangdong Province, China. Among them, 12 K. pneumoniae strains possessed high-level resistance to amikacin and gentamicin and these were screened for 16S rRNA methyltransferase (16S-RMTase) genes. And then the genes positive isolates were detected for ESBLs (extended spectrum β-lactamases) and analyzed by pulsed-field gel electrophoresis, multilocus sequence typing, PCR-based replicon typing and plasmid analysis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28446825/vaidyavallabha-an-authoritative-work-on-ayurveda-therapeutics
#12
REVIEW
Arhanth Kumar Jain, Shreevathsa Manjunath
The text "Vaidyavallabha" is an authoritative work on Ayurvedic therapeutics written by Hastiruci, a Jain scholar. It belongs to the time period of 1673-1726 CE. Different physical and mental ailments with their treatments are addressed in the 274 verses spanned over eight chapters in this work. In this text many unique, special and simple medicinal preparations for different diseases are given. Many drugs which were easily available in the local area are given much more importance in the treatment. Added to this, method and uniqueness of naming the diseases in the text stand differently when compared to other texts...
October 2016: Ancient Science of Life
https://www.readbyqxmd.com/read/28446742/work-and-health-care-in-milan-1864-1874
#13
Alessandro Porro, Lorenzo Lorusso, Bruno Falconi, Antonia Francesca Franchini
In 1864 in Milan, Giovanni Rosmini (1832-1896) opened an ophthalmic dispensary for workers. In 1874 this dispensary was transformed into the first ophthalmic hospital of the city. This hospital still exists today. The authors analyze a document that belonged to the lawyer Enrico Rosmini (1828-1898), brother of Giovanni, which helps to piece together the early years of the dispensary, where about 4,000 surgeries were carried out in the first four years of activity. This historical document is valuable as it sheds light on one of the first healthcare institution for workers in Milan...
April 21, 2017: La Medicina del Lavoro
https://www.readbyqxmd.com/read/28446675/comparative-genomics-of-chrysochromulina-ericina-virus-cev-and-other-microalgae-infecting-large-dna-viruses-highlight-their-intricate-evolutionary-relationship-with-the-established-mimiviridae-family
#14
Lucie Gallot-Lavallee, Guillaume Blanc, Jean-Michel Claverie
Chrysochromulina Ericina Virus CeV-01B (CeV) was isolated from Norwegian coastal waters in 1998. Its icosahedral particle is 160 nm in diameter and encloses a 474-kb dsDNA genome. This virus, although infecting a microalgae (the haptophyceae Haptolina ericina, formerly Chrysochromulina ericina), is phylogenetically related to members of the Mimiviridae family, initially established with the acanthamoeba-infecting Mimivirus and Megavirus as prototypes. This family was latter split into two genera (Mimivirus and Cafetariavirus) following the characterization of a virus infecting the heterotrophic stramenopile Cafeteria roenbergensis (CroV)...
April 26, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28446379/group-a-rotavirus-genotypes-in-hospital-acquired-gastroenteritis-in-italy-2012-14
#15
G Ianiro, R Delogu, L Fiore, M Monini, F M Ruggeri
BACKGROUND: Group A rotaviruses (RVA) are the leading cause of acute gastroenteritis (AGE) in young (aged <5 years) children, causing ∼250,000 deaths worldwide, mostly in developing countries. Differences on nucleotide sequences of VP7 (G-type) and VP4 (P-type) genes are the basis for the binary RVA nomenclature. Although at least 32 G-types and 47 P-types of rotavirus are presently known, most RVA infections in humans worldwide are related to five major G/P combinations: G1P[8], G2P[4], G3P[8], G4P[8], and G9P[8]...
April 8, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28446250/nanoparticles-based-on-essential-metals-and-their-phytotoxicity
#16
REVIEW
Branislav Ruttkay-Nedecky, Olga Krystofova, Lukas Nejdl, Vojtech Adam
Nanomaterials in agriculture are becoming popular due to the impressive advantages of these particles. However, their bioavailability and toxicity are key features for their massive employment. Herein, we comprehensively summarize the latest findings on the phytotoxicity of nanomaterial products based on essential metals used in plant protection. The metal nanoparticles (NPs) synthesized from essential metals belong to the most commonly manufactured types of nanomaterials since they have unique physical and chemical properties and are used in agricultural and biotechnological applications, which are discussed...
April 26, 2017: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/28445987/context-dependent-mir-204-and-mir-211-affect-the-biological-properties-of-amelanotic-and-melanotic-melanoma-cells
#17
Marianna Vitiello, Andrea Tuccoli, Romina D'Aurizio, Samanta Sarti, Laura Giannecchini, Simone Lubrano, Andrea Marranci, Monica Evangelista, Silvia Peppicelli, Chiara Ippolito, Ivana Barravecchia, Elena Guzzolino, Valentina Montagnani, Michael Gowen, Elisa Mercoledi, Alberto Mercatanti, Laura Comelli, Salvatore Gurrieri, Lawrence W Wu, Omotayo Ope, Keith Flaherty, Genevieve M Boland, Marc R Hammond, Lawrence Kwong, Mario Chiariello, Barbara Stecca, Gao Zhang, Alessandra Salvetti, Debora Angeloni, Letizia Pitto, Lido Calorini, Giovanna Chiorino, Marco Pellegrini, Meenhard Herlyn, Iman Osman, Laura Poliseno
Despite increasing amounts of experimental evidence depicting the involvement of non-coding RNAs in cancer, the study of BRAFV600E-regulated genes has thus far focused mainly on protein-coding ones. Here, we identify and study the microRNAs that BRAFV600E regulates through the ERK pathway.By performing small RNA sequencing on A375 melanoma cells and a vemurafenib-resistant clone that was taken as negative control, we discover miR-204 and miR-211 as the miRNAs most induced by vemurafenib. We also demonstrate that, although belonging to the same family, these two miRNAs have distinctive features...
April 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28445829/the-role-of-peu-mir164-and-its-target-penac-genes-in-response-to-abiotic-stress-in-populus-euphratica
#18
Xin Lu, Hui Dun, Conglong Lian, Xiaofei Zhang, Weilun Yin, Xinli Xia
Plant miR164 family is highly conserved and miR164 members regulate conserved targets belonging to NAC transcription factors. Our previous studies have revealed that peu-miR164a-e and its target gene POPTR_0007s08420 participate in abiotic stress response in Populus euphratica according to deep sequencing and degradome sequencing. In this study, miR164 family comprises six members that generate two mature products (miR164a-e and miR164f) and target seven NAC genes in P. euphratica. Co-expression in Nicotiana benthamiana and 5' RACE confirmed that peu-miR164 directs PeNAC070, PeNAC012 and PeNAC028 mRNAs cleavage...
April 8, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28445776/antimicrobial-resistance-in-the-bacteroides-fragilis-group-in-faecal-samples-from-patients-receiving-broad-spectrum-antibiotics
#19
Kia Cirkeline Møller Hansen, Simon A F Schwensen, Daniel Pilsgaard Henriksen, Ulrik Stenz Justesen, Thomas Vognbjerg Sydenham
Members of the Bacteroides fragilis group are opportunistic pathogens and cause severe infections including bacteraemia. As increased levels of antimicrobial resistance in B. fragilis group bacteria can be detected years after administration of specific antibiotics, monitoring antimicrobial susceptibility in the gut microbiota could be important. The objectives of this study were to 1) investigate the distribution of species and the occurrence of reduced antimicrobial susceptibility in the B. fragilis group from patients treated at departments with a high level of antibiotic use, 2) to determine the prevalence of the carbapenem resistance gene cfiA in B...
April 23, 2017: Anaerobe
https://www.readbyqxmd.com/read/28445701/prevalence-antimicrobial-susceptibility-and-multiplex-pcr-serotyping-of-listeria-monocytogenes-isolated-from-humans-foods-and-livestock-in-iran
#20
Lida Lotfollahi, Ardalan Chaharbalesh, Mohammad Ahangarzadeh Rezaee, Alka Hasani
Listeria monocytogenes is a foodborne pathogen causing listeriosis, which potentially affects all individuals, especially pregnant women and immunocompromised persons. The present study investigated the prevalence, antimicrobial susceptibility and serotypes distribution of the isolated L. monocytogenes from Iran. Twenty two (4.97%) of 442 human, food and livestock samples were found to be positive for L. monocytogenes. L. monocytogenes was identified in 8.8% of 125 human samples, 2.99% of 267 food and 6% of 50 livestock samples...
April 23, 2017: Microbial Pathogenesis
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