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https://www.readbyqxmd.com/read/28644516/hemostatic-efficiency-of-modern-topical-sealants-comparative-evaluation-after-liver-resection-and-splenic-laceration-in-a-swine-model
#1
Hamidreza Fonouni, Arash Kashfi, Ali Majlesara, Oliver Stahlheber, Lukas Konstantinidis, Negin Gharabaghi, Thomas W Kraus, Arianeb Mehrabi, Hani Oweira
Parenchymal transection during hepatobiliary surgery can disrupt small vasculature or bile ducts, which could be managed difficultly. Sealants are helpful tools to achieve better hemostasis. The aim of this study is to analyze the hemostatic efficiency of four modern sealants in a porcine model. In this study, 40 landrace pigs were assigned equally to the control (without sealant) and four sealant groups. Standardized liver resection and splenic lesions were performed and left without using sealant (control) or treated with one of the following sealants: TachoSil(®) , Tissucol Duo(®) , Coseal(®) , and FloSeal(®) ...
June 23, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28643045/validation-of-a-mathematical-model-for-laser-induced-thermotherapy-in-liver-tissue
#2
F Hübner, C Leithäuser, B Bazrafshan, N Siedow, T J Vogl
The purpose of the study was to develop a simulation approach for laser-induced thermotherapy (LITT) that is based on mathematical models for radiation transport, heat transport, and tissue damage. The LITT ablation was applied to ex vivo pig liver tissue. Experiments were repeated with different laser powers, i.e., 22-34 W, and flow rates of the cooling water in the applicator system, i.e., 47-92 ml/min. During the procedure, the temperature was measured in the liver sample at different distances to the applicator as well as in the cooling circuit using a fiber optic thermometer...
June 22, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28642489/post-exposure-treatment-of-non-human-primates-lethally-infected-with-ebola-virus-with-ebotab-a-purified-ovine-igg-product
#3
Stuart D Dowall, Frédéric Jacquot, John Landon, Emma Rayner, Graham Hall, Caroline Carbonnelle, Hervé Raoul, Delphine Pannetier, Ian Cameron, Ruth Coxon, Ibrahim Al Abdulla, Roger Hewson, Miles W Carroll
Despite sporadic outbreaks of Ebola virus (EBOV) over the last 4 decades and the recent public health emergency in West Africa, there are still no approved vaccines or therapeutics for the treatment of acute EBOV disease (EVD). In response to the 2014 outbreak, an ovine immunoglobulin therapy was developed, termed EBOTAb. After promising results in the guinea pig model of EBOV infection, EBOTAb was tested in the cynomolgus macaque non-human primate model of lethal EBOV infection. To ensure stringent therapeutic testing conditions to replicate likely clinical usage, EBOTAb was first delivered 1, 2 or 3 days post-challenge with a lethal dose of EBOV...
June 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28642298/does-acid-base-equilibrium-correlate-with-remnant-liver-volume-during-stepwise-liver-resection
#4
Mohammad Golriz, Sepehr Abbasi, Parham Fathi, Ali Majlesara, Thorsten Brenner, Arianeb Mehrabi
Small for size and flow syndrome (SFSF) is one of the most challenging complications following extended hepatectomy (EH). After EH, hepatic artery flow decreases and portal vein flow increases per 100 gram remnant liver volume (RLV). This causes hypoxia followed by metabolic acidosis. A correlation between acidosis and post-hepatectomy liver failure has been postulated, but not studied systematically in a large animal model or clinical setting. In our study, we performed stepwise liver resections on nine pigs to defined SFSF limits as follows: step 1: segment II/III resection, step 2: segment IV resection, step 3: segment V/VIII resection (RLV: 75%, 50% and 25% respectively)...
June 22, 2017: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/28633128/involvement-of-udp-glucuronosyltransferases-in-higenamine-glucuronidation-and-the-gender-and-species-differences-in-liver
#5
Zhi Liang, Chang Xu, Lingna Dong, Yile Fu, Qiong Wu, Jie Zhao, Ling Ye, Zheng Cai, Menghua Liu, Bijun Xia, Lan Tang, Zhongqiu Liu
OBJECTIVES: Higenamine (HG), an active ingredient of Aconite root in Chinese herbal medicine, is mainly metabolized by UDP-glucuronosyltransferases (UGT). However, the systematic glucuronidation of HG in humans remains unclear. The purpose of this study was to investigate the glucuronidation of HG. METHODS: 12 recombinant human UGT (rUGT) isozymes were used to characterize the HG glucuronidation. Liver microsomes from male and female mice, rats, guinea pigs, dogs, and humans were used to determine the species and gender differences using liquid chromatography-mass spectrometry...
June 17, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28630420/functional-evaluation-of-a-bioartificial-liver-support-system-using-immobilized-hepatocyte-spheroids-in-a-porcine-model-of-acute-liver-failure
#6
Ji-Hyun Lee, Doo-Hoon Lee, Sanghoon Lee, Choon Hyuck David Kwon, Jae-Nam Ryu, Jeong-Kwon Noh, In Keun Jang, Hey-Jung Park, Hee-Hoon Yoon, Jung-Keug Park, Young-Jin Kim, Sung-Koo Kim, Suk-Koo Lee
Bioartificial livers (BAL) may offer acute liver failure (ALF) patients an opportunity for cure without liver transplantation. We evaluated the efficacy of a spheroid-based BAL system, containing aggregates of porcine hepatocytes, in a porcine model of ALF. ALF pigs were divided into three groups. The control group consisted of treatment naïve pigs (n = 5), blank group consisted of pigs that were attached to the BAL system not containing hepatocytes for 12 hours (n = 5) and BAL group consisted of pigs that were attached to the BAL containing hepatocytes for 12 hours (n = 5)...
June 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28630341/pig-model-mimicking-chronic-hepatitis-e-virus-infection-in-immunocompromised-patients-to-assess-immune-correlates-during-chronicity
#7
Dianjun Cao, Qian M Cao, Sakthivel Subramaniam, Danielle M Yugo, C Lynn Heffron, Adam J Rogers, Scott P Kenney, Debin Tian, Shannon R Matzinger, Christopher Overend, Nicholas Catanzaro, Tanya LeRoith, Heng Wang, Pablo Piñeyro, Nicole Lindstrom, Sherrie Clark-Deener, Lijuan Yuan, Xiang-Jin Meng
Chronic hepatitis E virus (HEV) infection is a significant clinical problem in immunocompromised individuals such as organ transplant recipients, although the mechanism remains unknown because of the lack of an animal model. We successfully developed a pig model of chronic HEV infection and examined immune correlates leading to chronicity. The conditions of immunocompromised patients were mimicked by treating pigs with an immunosuppressive regimen including cyclosporine, azathioprine, and prednisolone. Immunocompromised pigs infected with HEV progressed to chronicity, because 8/10 drug-treated HEV-infected pigs continued fecal virus shedding beyond the acute phase of infection, whereas the majority (7/10) of mock-treated HEV-infected pigs cleared fecal viral shedding at 8 wk postinfection...
June 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28606764/transcriptional-profiling-of-male-f344-rats-suggests-the-involvement-of-calcium-signaling-in-the-mode-of-action-of-acrylamide-induced-thyroid-cancer
#8
Nikolai L Chepelev, Rémi Gagné, Timothy Maynor, Byron Kuo, Cheryl A Hobbs, Leslie Recio, Carole L Yauk
Acrylamide (AA) exposure in 2-year cancer bioassays leads to thyroid, but not liver, adenomas and adenocarcinomas in rats. Hypothesized modes of action (MOAs) include genotoxicity/mutagenicity, or thyroid hormone dysregulation. To examine the plausibility of these two, or any alternative MOAs, RNA-sequencing was performed on the thyroids and livers of AA-exposed rats, in parallel with measurement of genotoxicity (blood micronucleus and Pig-a mutant frequency) and serum thyroid hormone levels, following the exposure of male Fischer 344/DuCrl rats to 0...
June 9, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28588496/clinical-efficacy-and-residue-depletion-of-10-enrofloxacin-enteric-coated-granules-in-pigs
#9
Zhixin Lei, Qianying Liu, Bing Yang, Jincheng Xiong, Kun Li, Saeed Ahmed, Liping Hong, Pin Chen, Qigai He, Jiyue Cao
A new, more palatable formulation of 10% enrofloxacin enteric-coated granules was investigated to evaluate the pharmacokinetic effect in plasma, the residue elimination in tissues and the clinical efficacy against Actinobacillus pleuropneumonia (APP) and Mycoplasam suis (MS) in pigs. In this study, the enrofloxacin concentrations in plasma and tissues were detected using high-performance liquid chromatography with phosphate buffer (pH = 3) and acetonitrile. The pharmacokinetics and elimination of enrofloxacin enteric-coated granules were performed after oral administration at a single dose of 10 mg/kg body weight (bw) and 5 mg/kg twice per day for 5 consecutive days, respectively...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28585425/cide-gene-expression-in-adipose-tissue-liver-and-skeletal-muscle-from-obese-and-lean-pigs
#10
Yue-Qin Qiu, Xue-Fen Yang, Xian-Yong Ma, Yun-Xia Xiong, Zhi-Mei Tian, Qiu-Li Fan, Li Wang, Zong-Yong Jiang
The expression of the cell death-inducing DNA fragmentation factor α-like effector (CIDE) family including Cidea, Cideb, and Cidec was significantly increased in mouse and human models of obesity. However, there was less information on these genes' expression in pigs. Here, we hypothesized that different fat accumulation between lean (Duroc×Landrace×Yorkshire gilts, DLY) and obese (Lantang) pigs was attributed to porcine CIDE-modulating lipid metabolism. Our data showed that Cidea and Cidec were expressed at a high level in adipose tissue, and at a relatively high level in skeletal muscle, whereas Cideb was mainly expressed in the liver in both breeds of pig...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28572613/mesenchymal-stem-cells-correct-haemodynamic-dysfunction-associated-with-liver-injury-after-extended-resection-in-a-pig-model
#11
Hans-Michael Tautenhahn, Sandra Brückner, Christiane Uder, Silvio Erler, Madlen Hempel, Martin von Bergen, Janine Brach, Sandra Winkler, Franziska Pankow, Claudia Gittel, Manja Baunack, Undine Lange, Johannes Broschewitz, Matthias Dollinger, Michael Bartels, Uta Pietsch, Kerstin Amann, Bruno Christ
In patients, acute kidney injury (AKI) is often due to haemodynamic impairment associated with hepatic decompensation following extended liver surgery. Mesenchymal stem cells (MSCs) supported tissue protection in a variety of acute and chronic diseases, and might hence ameliorate AKI induced by extended liver resection. Here, 70% liver resection was performed in male pigs. MSCs were infused through a central venous catheter and haemodynamic parameters as well as markers of acute kidney damage were monitored under intensive care conditions for 24 h post-surgery...
June 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28568482/diffusion-limited-pbpk-model-for-predicting-pulmonary-pharmacokinetics-of-florfenicol-in-pig
#12
M R Qian, Q Y Wang, H Yang, G Z Sun, X B Ke, L L Huang, J D Gao, J J Yang, B Yang
For most bacterial lung infections, the concentration of unbound antimicrobial agent in lung interstitial fluid has been thought to be responsible for antimicrobial efficacy. In this study, a diffusion-limited physiologically based pharmacokinetic (PBPK) model was developed to predict the pulmonary pharmacokinetics of florfenicol (FF) in pigs. The model included separate compartments corresponding to blood, diffusion-limited lung, flow-limited muscle, liver, and kidney and an extra compartment representing the remaining carcass...
June 1, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28566885/artificial-liver-support-in-pigs-with-acetaminophen-induced-acute-liver-failure
#13
Guo-Lin He, Lei Feng, Lei Cai, Chen-Jie Zhou, Yuan Cheng, Ze-Sheng Jiang, Ming-Xin Pan, Yi Gao
AIM: To establish a reversible porcine model of acute liver failure (ALF) and treat it with an artificial liver system. METHODS: Sixteen pigs weighing 30-35 kg were chosen and administered with acetaminophen (APAP) to induce ALF. ALF pigs were then randomly assigned to either an experimental group (n = 11), in which a treatment procedure was performed, or a control group (n = 5). Treatment was started 20 h after APAP administration and continued for 8 h. Clinical manifestations of all animals, including liver and kidney functions, serum biochemical parameters and survival times were analyzed...
May 14, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28564593/the-occurrence-of-l-lactate-dehydrogenase-in-the-inner-mitochondrial-compartment-of-pig-liver
#14
Gianluca Paventi, Roberto Pizzuto, Salvatore Passarella
Although pig represents a model species in biomedical research including studies dealing with liver patho-physiology, some aspects of liver metabolism need to be addressed. In particular, whether and how pig mitochondria can metabolize l-lactate remains to be established. We show here that pig liver mitochondria (PLM) possess their own l-lactate dehydrogenase (mL-LDH). This was shown both via immunological analysis and by assaying photometrically the L-LDH reaction in solubilised PLM. The mL-LDH reaction shows hyperbolic dependence on the substrate concentration, it is inhibited by oxamate and proves to differ from the cytosolic activity (cL-LDH), as revealed by the difference found in both pH profiles and temperature dependence of m- and cL-LDH...
May 28, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28562823/transcolonic-perirectal-notes-access-pna-a-feasibility-study-with-survival-in-swine-model
#15
André L A Oliveira, Ricardo Zorron, Flavio M M DE Oliveira, Marcelo B Dos Santos, Jussara P Scheffer, Marcelo Rios, Fernanda Antunes
Transrectal access still has some unsolved issues such as spatial orientation, infection, access and site closure. This study presents a simple technique to perform transcolonic access with survival in a swine model series. A new technique for NOTES perirectal access to perform retroperitoneoscopy, peritoneoscopy, liver and lymphnode biopsies was performed in 6 pigs, using Totally NOTES technique. The specimens were extracted transanally. The flexible endoscope was inserted through a posterior transmural incision and the retrorectal space...
May 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28562413/renal-adiposity-confounds-quantitative-assessment-of-markers-of-renal-diffusion-with-mri-a-proposed-correction-method
#16
Behzad Ebrahimi, Ahmed Saad, Kai Jiang, Christopher M Ferguson, Hui Tang, John R Woollard, James F Glockner, Stephen C Textor, Lilach O Lerman
OBJECTIVES: Recent studies have indicated that excessive fat may confound assessment of diffusion in organs with high fat content, such as the liver and breast. However, the extent of this effect in the kidney, which is not considered a major fat deposition site, remains unclear. This study tested the hypothesis that renal fat may impact diffusion-weighted imaging (DWI) parameters, and proposes a 3-compartment model (TCM) to circumvent this effect. METHODS: Using computer simulations, we investigated the effect of fat on assessment of apparent diffusion coefficient (ADC), intravoxel incoherent motion (IVIM), and TCM-derived pure-diffusivity...
May 29, 2017: Investigative Radiology
https://www.readbyqxmd.com/read/28557354/modeling-liver-electrical-conductivity-during-hypertonic-injection
#17
Quim Castellví, Patricia Sánchez-Velázquez, Xavier Moll, Enrique Berjano, Anna Andaluz, Fernando Burdío, Bart Bijnens, Antoni Ivorra
Metastases in the liver frequently grow as scattered tumor nodules which neither can be removed by surgical resection nor focally ablated. Previously, we have proposed a novel technique based on irreversible electroporation which may be able to simultaneously treat all nodules in the liver while sparing healthy tissue. The proposed technique requires increasing the electrical conductivity of healthy liver by injecting a hypersaline solution through the portal vein. Aiming to assess the capability of increasing the global conductivity of the liver by means of hypersaline fluids, here it is presented a mathematical model which estimates the NaCl distribution within the liver and the resulting conductivity change...
May 30, 2017: International Journal for Numerical Methods in Biomedical Engineering
https://www.readbyqxmd.com/read/28549200/effects-of-cell-death-inducing-dff45-like-effector-b-on-meat-quality-traits-in-berkshire-pigs
#18
G E Yu, S Kwon, J H Hwang, S M An, D H Park, D G Kang, T W Kim, I-S Kim, H C Park, J Ha, C W Kim
Cell death-inducing DFF45-like effector (CIDE) B is a member of the CIDE family of apoptosis-inducing factors. In the present study, we detected a single nucleotide polymorphism (SNP), c.414G>A, which corresponds to the synonymous SNP 414Arg, in CIDE-B in the Berkshire pigs. We also analyzed the relationships between the CIDE-B SNP and various meat quality traits. The SNP was significantly associated with post-mortem pH24h, water-holding capacity (WHC), fat content, protein content, drip loss, post-mortem temperature at 12 h (T12) and 24 h (T24) in a co-dominant model (P < 0...
May 25, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28547928/application-of-endobronchial-ultrasonography-in-laparoscopic-liver-segmentectomy-in-an-animal-model
#19
Tomoyuki Abe, Tsuyoshi Kobayashi, Seiichi Shimizu, Michinori Hamaoka, Hiroshi Iwako, Masakazu Hashimoto, Yoshihiro Mikuriya, Shintaro Kuroda, Hirotaka Tashiro, Hideki Ohdan
INTRODUCTION: This study investigated whether laparoscopic ultrasound-guided segment staining and real-time ultrasound-guided hepatectomy, with endobronchial ultrasonography equipped with a guide sheath, would be useful for laparoscopic liver segmentectomy in a porcine model. MATERIAL AND SURGICAL TECHNIQUE: The abdominal cavity (in two pigs) was reached via a 12-mm umbilical trocar. An artificial tumor was created by radiofrequency ablation within the intended resection area...
May 2017: Asian Journal of Endoscopic Surgery
https://www.readbyqxmd.com/read/28507824/non-invasive-radiofrequency-field-treatment-to-produce-hepatic-hyperthermia-efficacy-and-safety-in-swine
#20
Jason C Ho, Lam Nguyen, Justin J Law, Matthew J Ware, V Keshishian, N C Lara, Trac Nguyen, Steven A Curley, Stuart J Corr
The Kanzius non-invasive radio-frequency hyperthermia system (KNiRFH) has been investigated as a treatment option for hepatic hyperthermia cancer therapy. The treatment involves exposing the patient to an external high-power RF (13.56 MHz) electric field, whereby the propagating waves penetrate deep into the tumor causing targeted heating based on differential tissue dielectric properties. However, a comprehensive examination of the Kanzius system alongside any associated toxicities and its ability to induce hepatic hyperthermia in larger animal models, such as swine, are the subjects of the work herein...
2017: IEEE Journal of Translational Engineering in Health and Medicine
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