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https://www.readbyqxmd.com/read/28737824/inhibitory-effects-of-1-25-oh-2d3-on-the-proliferation-of-hepatocellular-carcinoma-cells-through-the-downregulation-of-hdac2
#1
Jian Huang, Guozhen Yang, Yunzhu Huang, Shu Zhang
The inhibitory effects of 1,25(OH)2D3 on the proliferation of a variety of cancer cell lines have been extensively reported. However, the underlying mechanisms remain largely unknown. In the present study, the effects of 1,25(OH)2D3 on the in vitro proliferation of human hepatocellular carcinoma HepG2 cells and the mechanism involved were investigated. Flow cytometry and MTT assay revealed that 1,25(OH)2D3 inhibited cell proliferation in vitro. Western blotting and real-time PCR indicated that 1,25(OH)2D3 upregulated the expression of phosphatase and tensin homologue deleted on chromosome 10 (PTEN) and attenuated that of histone deacetylase 2 (HDAC2)...
July 24, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28737815/isofraxidin-a-coumarin-component-improves-high-fat-diet-induced-hepatic-lipid-homeostasis-disorder-and-macrophage-inflammation-in-mice
#2
Jian Li, Xiaofei Li, Zhike Li, Lu Zhang, Yonggang Liu, Hong Ding, Shanye Yin
Isofraxidin (IF) is a coumarin compound produced in the functional foods Siberian ginseng and Apium graveolens. The first objective of this study was to investigate the protective effects and putative methods of IF in combating lipotoxicity in vitro and in vivo. Oleic acid was used to induce lipid turbulence in human hepatoma cells (HepG2). Alterations in triglyceride metabolism, inflammation and oxidative status were monitored. Results show that IF mainly reduced triglyceride accumulation, TNF-α release and ROS activation in metabolic disordered cells...
July 24, 2017: Food & Function
https://www.readbyqxmd.com/read/28737396/azacyclo-indoles-and-phenolics-from-the-flowers-of-juglans-regia
#3
Qian Li, An-Jun Deng, Li Li, Lian-Qiu Wu, Ming Ji, Hai-Jing Zhang, Zhi-Hong Li, Lin Ma, Zhi-Hui Zhang, Xiao-Guang Chen, Hai-Lin Qin
Seven new azacyclo-indoles and phenolics and four known alkaloids were isolated from the flowers of Juglans regia. Spectroscopic and chromatographic data revealed that the structures of the new compounds are 5,6,11,12-tetrahydropyrrolo[1',2':1,2]azepino[4,5-b]indole-3-carbaldehyde (1), (±)-5,6,7,11c-tetrahydro-1H-indolizino[7,8-b]indol-3(2H)-one (2), (±)-9-hydroxy-5-oxo-2,3,4,5-tetrahydro-1H-benzo[b]azepine-2-carboxamide (3), 5-(ethoxymethyl)-1-(4-hydroxyphenethyl)-1H-pyrrole-2-carbaldehyde (4), (±)-5,8-dihydroxy-4-(1H-indol-3-yl)-3,4-dihydronaphthalen-1(2H)-one (5), (±)-4-(6-amino-9H-purin-9-yl)-5,8-dihydroxy-3,4-dihydronaphthalen-1(2H)-one (6), and (±)-4-(6-amino-9H-purin-9-yl)-5-hydroxy-3,4-dihydronaphthalen-1(2H)-one (7)...
July 24, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28736283/multicellular-dosimetric-chain-for-molecular-radiotherapy-exemplified-with-dose-simulations-on-3d-cell-spheroids
#4
Vappu Reijonen, Liisa K Kanninen, Eero Hippeläinen, Yan-Ru Lou, Eero Salli, Alexey Sofiev, Melina Malinen, Timo Paasonen, Marjo Yliperttula, Antti Kuronen, Sauli Savolainen
PURPOSE: Absorbed radiation dose-response relationships are not clear in molecular radiotherapy (MRT). Here, we propose a voxel-based dose calculation system for multicellular dosimetry in MRT. We applied confocal microscope images of a spherical cell aggregate i.e. a spheroid, to examine the computation of dose distribution within a tissue from the distribution of radiopharmaceuticals. METHODS: A confocal microscope Z-stack of a human hepatocellular carcinoma HepG2 spheroid was segmented using a support-vector machine algorithm and a watershed function...
July 20, 2017: Physica Medica: PM
https://www.readbyqxmd.com/read/28735729/protective-effects-of-flavonoids-isolated-from-korean-milk-thistle-cirsium-japonicum-var-maackii-maxim-matsum-on-tert-butyl-hydroperoxide-induced-hepatotoxicity-in-hepg2-cells
#5
Hyun Ah Jung, Qudeer Ahmed Abdul, Jeong Su Byun, Eun-Ji Joung, Wi-Gyeong Gwon, Min-Sup Lee, Hyeung Rak Kim, Jae Sue Choi
ETHNOPHARMACOLOGICAL RELEVANCE: Milk thistle leaves and flowers have been traditionally used as herbal remedy to alleviate liver diseases for decades. Korean milk thistle, Cirsium japonicum var. maackii (Maxim.) Matsum has been employed in traditional folk medicine as diuretic, antiphlogistic, hemostatic, and detoxifying agents. AIM OF THE STUDY: The aim of current investigation was to evaluate hepatoprotective properties of the MeOH extract of the roots, stems, leaves and flowers of Korean milk thistle as well as four isolated flavonoids, luteolin, luteolin 5-O-glucoside, apigenin and apigenin 7-O-glucuronide during t-BHP-induced oxidative stress in HepG2 cells...
July 20, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28734833/transforming-growth-factor-beta-promotes-liver-tumorigenesis-in-mice-via-upregulation-of-snail
#6
Hyuk Moon, Hye-Lim Ju, Sook In Chung, Kyung Joo Cho, Jung Woo Eun, Suk Woo Nam, Kwang-Hyub Han, Diego F Calvisi, Simon Weonsang Ro
BACKGROUND & AIMS: Transforming growth factor beta (TGFB) suppresses early stages of tumorigenesis, but also contributes to migration and metastasis of cancer cells. A large number of human tumors contain mutations that inactivate its receptors, or downstream proteins such as Smad transcription factors, indicating that the TGFB signaling pathway prevents tumor growth. We investigated the effects of TGFB inhibition on liver tumorigenesis in mice. METHODS: C57BL/6 mice received hydrodynamic tail injections of transposons encoding HRAS(G12V) and a short hairpin RNA (shRNA) to downregulate p53, or those encoding HRAS(G12V) and MYC, or those encoding HRAS(G12V) and TAZ(S89), to induce liver tumor formation; mice were also given injections of transposons encoding SMAD7 or shRNA against SMAD2, SMAD3, SMAD4, or SNAI1 (Snail), with or without ectopic expression of Snail...
July 19, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28734040/microrna-374a-promotes-hepatocellular-carcinoma-cell-proliferation-by-targeting-mitogen-inducible-gene-6-mig-6
#7
Hui Li, Huicheng Chen, Haibin Wang, Yilong Dong, Min Yin, Liang Zhang, Jia Wei
Hepatocellular carcinoma (HCC) is a dreadful disease with poor prognosis rates and ineffective therapeutic options. Previous studies have reported the involvement of mitogeninducible gene 6 (MIG-6) as a negative regulator in tumor formation. MicroRNAs (miRNAs) play crucial roles in the development of different types of cancer. However, the underlying mechanisms of miRNAs in HCC are poorly understood. This study was aimed to investigate the role of miR-374a in HCC and its role in regulation of expression of MIG-6...
July 21, 2017: Oncology Research
https://www.readbyqxmd.com/read/28733231/tussilagonone-induced-nrf2-pathway-activation-protects-hepg2-cells-from-oxidative-injury
#8
Kyung-Mi Lee, Tae Yeon Kwon, Unwoo Kang, Eun Kyoung Seo, Ji Ho Yun, Chu Won Nho, Yeong Shik Kim
Tussilagonone is a compound derived from the medicinal plant Tussilago farfara L., which is used as a traditional medicine for respiratory diseases, including asthma and pneumonia. Recent reports suggest that tussilagonone exhibits anti-inflammatory effects; however, the scope of protective functions has not been elucidated yet. In this study, we demonstrate that tussilagonone enhances cellular detoxification by increasing quinone reductase activity in Hepa1c1c7 cells. In addition, tussilagonone decreased tert-butyl hydroperoxide(t-BHP)-induced ROS production and cell death, suggesting that it also acts as a potent antioxidant...
July 18, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28733206/omics-based-identification-of-the-combined-effects-of-idiosyncratic-drugs-and-inflammatory-cytokines-on-the-development-of-drug-induced-liver-injury
#9
J Jiang, K Mathijs, L Timmermans, S M Claessen, A Hecka, J Weusten, R Peters, J H van Delft, J C S Kleinjans, D G J Jennen, T M de Kok
The mechanisms of idiosyncratic drug-induced hepatotoxicity remain largely unclear. It has demonstrated that the drug idiosyncrasy is potentiated in the context of inflammation and intracellular ceramides may play a role in this process. To study the mechanisms, HepG2 cells were co-treated with high and low doses of three idiosyncratic (I) and three non-idiosyncratic (N) compounds, with (I+ and N+) or without (I- and N-) a cytokine mix. Microarray, lipidomics and flow cytometry were performed to investigate the genome-wide expression patterns, the intracellular ceramide levels and the induction of apoptosis...
July 18, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28733192/pharmacological-evaluation-of-phytochemicals-from-south-indian-black-turmeric-curcuma-caesia-roxb-to-target-cancer-apoptosis
#10
K S Mukunthan, R S Satyan, T N Patel
Curcuma caesia Roxb. (Black turmeric), a perennial herb of the family Zingiberaceae is indigenous to India. C. caesia is used as a spice, food preservative and coloring agent commonly in the Indian subcontinent. Functional parametric pharmacological evaluations like drug ability and toxicity profile of this endangered species is poorly documented. In our present study, among all the extracts of dried C. caesia rhizome viz- hexane, ethyl acetate, methanol and water tested for free radical scavenging capacity by total antioxidant activity (TAO) method, Hexane Rhizome Extract (HRE) wzs found to possess remarkable activity (1200mg ascorbic acid equivalent/100g)...
July 18, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28732920/a-rhamnan-type-sulfated-polysaccharide-with-novel-structure-from-monostroma-angicava-kjellm-chlorophyta-and-its-bioactivity
#11
Xue Liu, Jiejie Hao, Xiaoxi He, Shuyao Wang, Sujian Cao, Ling Qin, Wenjun Mao
A homogeneous polysaccharide was obtained from Monostroma angicava Kjellm by water extraction, preparative anion-exchange and size-exclusion chromatography. Results of chemical and spectroscopic analyses showed that the polysaccharide was a glucuronic acid-containing rhamnan-type sulfated polysaccharide. The backbone mainly consisted of →3)-α-l-Rhap-(1→ and →2)-α-l-Rhap-(1→ residues, partially sulfated at C-2 of →3)-α-l-Rhap-(1→ and C-3/C-4 of →2)-α-l-Rhap-(1→. The branching contained unsulfated or monosulfated 3-linked, 2-linked, 4-linked α-l-rhamnose and terminal β-d-glucuronic acid residues...
October 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28732914/spermine-modified-starch-based-carrier-for-gene-delivery-structure-transfection-activity-relationships
#12
Xiaoyi Huang, Xiaoxi Li, Ling Chen, Lin Li
This study aims at developing starch based gene carriers with low cytotoxicity and high transfection efficiency. Starch molecules with molecular weight of about 50kDa were cationically modified by spermine to obtain spermine modified starch (SMS) based gene carriers. Plasmid pAcGFP1-C1 (pDNA) was chosen as the model gene material and formed self-assembly nanocomplexes with SMS. The cytotoxicity and transfection efficiency of SMS/pDNA complexes were tested in HepG2 cell lines. Results showed that SMS/pDNA complexes formed by SMS-DS3 with the highest primary amine content (1...
October 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28732911/toxicity-of-native-and-oxovanadium-iv-v-galactomannan-complexes-on-hepg2-cells-is-related-to-impairment-of-mitochondrial-functions
#13
Monique Meyenberg Cunha-de Padua, Silvia Maria Suter Correia Cadena, Carmen Lucia de Oliveira Petkowicz, Glaucia Regina Martinez, Maria Eliane Merlin Rocha, Ana Lucia Ramalho Mercê, Guilhermina Rodrigues Noleto
Polysaccharides and vanadium compounds have been studied due to their antitumor potential. In this study, the cytotoxic effects of galactomannan preparations on HepG2 cells were investigated. Native galactomannan from S. amazonicum (SAGM) and its modified form (MSAGM) were complexed with oxovanadium resulting in SAGM:VO and MSAGM:VO, respectively. The complexation was confirmed by NMR, FTIR, and AAS. SAGM and MSAGM:VO (250μg/mL) after 72h decreased viability by 51% and 58%, respectively, while the inhibition of the HepG2 cell proliferation was of ∼27% and ∼46%, respectively...
October 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28732860/structure-features-and-in-vitro-hypoglycemic-activities-of-polysaccharides-from-different-species-of-maidong
#14
Yajun Gong, Jie Zhang, Fei Gao, Jiewen Zhou, Zhinan Xiang, Chenggao Zhou, Luosheng Wan, Jiachun Chen
Structures and in vitro hypoglycemic activities of polysaccharides from different species of Maidong were studied. The primary structures of polysaccharides were elucidated on the basis of GC, GC-MS, infrared, NMR and periodate oxidation-Smith degradation. Liriope spicata polysaccharide (LSP), Ophiopogon japonicus polysaccharide (OJP) and Liriope muscari polysaccharide (LMP) were composed of β-fructose and α-glucose. The average molecular weights of LSP, OJP and LMP were 4742, 4925 and 4138Da with polydispersity indexes of 1...
October 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28732858/optimization-of-microwave-assisted-extraction-of-sargassum-thunbergii-polysaccharides-and-its-antioxidant-and-hypoglycemic-activities
#15
Beibei Ren, Chun Chen, Chao Li, Xiong Fu, Lijun You, Rui Hai Liu
In the present work, optimization of microwave-assisted extraction of polysaccharides from Sargassum thunbergii was investigated. The physicochemical properties, structural characteristics, and in vitro antioxidant and hypoglycemic activities were determined. The optimal extraction conditions with a yield of 2.84±0.09% for extraction of polysaccharides (STP-1) were extraction time 23min, microwave power 547W, extraction temperature 80°C, and the ratio of raw material to water 1:27g/mL. STP-1 contained 32...
October 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28732575/beraprost-sodium-a-prostacyclin-analogue-reduces-fructose-induced-hepatocellular-steatosis-in-mice-and-in-vitro-via-the-microrna-200a-and-sirt1-signaling-pathway
#16
Pengyuan Zhang, Lijuan Xu, Hongyu Guan, Liehua Liu, Juan Liu, Zhimin Huang, Xiaopei Cao, Zhihong Liao, Haipeng Xiao, Yanbing Li
PURPOSE: To determine whether beraprost sodium, a prostacyclin analogue, could reduce hepatic lipid accumulation induced by fructose in mice and cultured human hepatocytes, and to investigate the expression of microRNAs and the sirtuin 1 (SIRT1) pathway. METHODS: Male C57BL/6JNju mice were divided into three groups and fed one of the following diets: a normal diet, a high fructose diet, or a high fructose diet with beraprost sodium treatment. In addition, human-derived HepG2 cells were cultured and treated with fructose (25mmol/L) with or without beraprost sodium (10μmol/L) for 24h, and transfected with small interfering RNA (siRNA) against SIRT1, miR-200a mimic, or miR-200a inhibitor for 48h...
August 2017: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/28732404/influence-of-the-stabilizers-on-the-toxicity-of-metallic-nanomaterials-in-aquatic-organisms-and-human-cell-lines
#17
Tatiana Andreani, Verónica Nogueira, Vera V Pinto, Maria José Ferreira, Maria Graça Rasteiro, Amélia M Silva, Ruth Pereira, Carlos M Pereira
In this study, following a systematic approach, we used aquatic species (bacteria Vibrio fischeri and microalgae Raphidocelis subcapitata) and different human cell lines (Caco-2, HepG2, SV-80 and HaCaT) representing different tissues and exposure pathways, to investigate how two organic stabilizers (PVA and DMSO) used for NMs dispersion influence their physicochemical properties, the persistence of metals in suspension and the toxicity/ecotoxicity of two metallic NMs (nano-Ag and nano-Cu). Although the stabilizers are expected to contribute to improve the dispersion and stability of NMs, the results obtained clearly showed that no similar changes in toxicity and morphological properties of the nano-Ag can be expected after its stabilization with PVA...
July 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28732393/microrna-30a-suppresses-tumor-progression-by-blocking-ras-raf-mek-erk-signaling-pathway-in-hepatocellular-carcinoma
#18
Kun Zhou, Xiaoyu Luo, Yu Wang, Dachun Cao, Gang Sun
Emerging reports suggest microRNAs (miRNAs) play a vital role in the progression of malignant tumors. MiR-30a is downregulated in a variety of cancers and acts as a tumor suppressing gene. However, the molecular mechanisms of miRNA-30a in hepatocellular carcinoma (HCC) are still unclear. Hereby, in this study, we detected that miR-30a expression was significantly down-regulated in both HCC tissues compared with adjacent non-cancerous liver tissues, and we also observed that miR-30a expression was lower in HCC cell lines than that of normal controls...
July 17, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28731233/integration-of-cellular-and-molecular-endpoints-to-assess-the-toxicity-of-polycyclic-aromatic-hydrocarbons-in-hepg2-cell-line
#19
Patrícia I Morgado, Sílvia Jose, Riccardo Wanke, Alexandra M M Antunes, Ana Sofia Cardoso, Luisa Jordao
Polycyclic aromatic hydrocarbons (PAHs) are persistent pollutants present in the environment with known mutagenic and carcinogenic properties. In the present study the effect of exposure to single or multiple doses of benzo[a]anthracene (BaA), pyrene (Pyr) and three halogenated derivatives of these compounds (1-ClPyr; 1-BrPyr and 7-ClBaA) were evaluated in a liver-derived human cell line (HepG2). Cytotoxicity as assessed by the classic MTT and neutral red assays showed a mild toxic effect in response to single or multiple dose exposure for up to 72h; except for multiple dose exposure to BaA and 7-ClBaA (1μM per day during 4 days) and single exposure to 10 μM BaA...
July 21, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28731159/ganoderic-acid-a-inhibits-proliferation-and-invasion-and-promotes-apoptosis-in-human-hepatocellular-carcinoma-cells
#20
Xu Wang, Di Sun, Jiandong Tai, Lei Wang
Ganoderic acid A (GA‑A), a triterpenoid, has been demonstrated to suppress cell proliferation in various cancers, including breast cancer and osteosarcoma. However, its effect on human hepatocellular carcinoma (HCC) remains to be elucidated. The present study aimed to investigate the effect of GA‑A on HCC cells in vitro. The HepG2 and SMMC7721 human HCC cell lines were treated with differing concentrations of GA‑A for 24, 48 and 72 h. The cell growth rate, cell cycle and apoptosis, migration and invasion were determined using a Cell Counting Kit‑8, flow cytometry and transwell assays, respectively...
July 21, 2017: Molecular Medicine Reports
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