keyword
MENU ▼
Read by QxMD icon Read
search

Hepg2 cells

keyword
https://www.readbyqxmd.com/read/28212581/the-inhibiting-effect-of-the-transcription-factor-p53-on-dengue-virus-infection-by-activating-the-type-i-interferon
#1
Yan-Ling Hu, Xiao-Shan Li, Shu Xiong, Qiang Ma, Dan Liu, Zhong-Quan Shi, Jing Tang, Xian-Cai Rao, Fu-Quan Hu, Guo-Li Li
To investigate the role of the transcription factor p53 in the course of the dengue virus (DV) infection. The human hepatocellular carcinoma cell strain HepG2 with a low expression level of p53 was built by using the retroviral-mediated RNA interference technology, and was detected by Western blot. The wild group and the interference group were respectively infected by the type 2 DV. The viral titration was detected by the Vero plaque assay, the viral multiplication was detected by the immunofluorescence, the cell apoptosis after virus infection was detected by FCM and the level of IFN-β was analyzed by ELISA...
February 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28209522/the-role-of-alkaline-phosphatase-in-intracellular-lipid-accumulation-in-the-human-hepatocarcinoma-cell-line-hepg2
#2
George M Chirambo, Chantal van Niekerk, Nigel J Crowther
Inhibition of tissue non-specific alkaline phosphatase (TNALP) decreases intracellular lipid accumulation in human preadipocytes and the murine preadipocyte cell line, 3T3-L1. Therefore, the current study was performed to determine if TNALP is required for intracellular lipid deposition in the human hepatocyte cell line, HepG2. Intracellular lipid accumulation, TNALP activity and peroxisome proliferator activated receptor (PPAR) γ gene expression were measured in HepG2 and 3T3-L1 cells in the presence and absence of the TNALP inhibitors levamisole and histidine...
February 13, 2017: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/28208247/quantum-dots-labeling-strategy-for-counting-and-visualization-of-hepg2-cells
#3
Bin Yang, Beibei Chen, Man He, Bin Hu
We report a sensitive, selective, simple, and reliable magnetic immunoassay protocol for detection and imaging of HepG2 cells. After being captured by Cs-doped multicore magnetic nanoparticles (MMNPs), HepG2 cells were labeled by CdSe/ZnS quantum dots (QDs), which could be visualized by fluorescence imaging using the photoluminescence property of QDs, and subsequently, they can be counted by inductively coupled plasma mass spectrometry (ICP-MS) with Cd/Cs as elemental tag. Because of the superior photoluminescence properties and the large quantities of detectable Cd atoms contained in the QDs core, QDs play a dual function role in this assay, making the method easier and more comprehensive than other similar approaches...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28208244/fluorescein-based-chromogenic-and-ratiometric-fluorescence-probe-for-highly-selective-detection-of-cysteine-and-its-application-in-bioimaging
#4
Zhen-Hai Fu, Xiao Han, Yongliang Shao, Jianguo Fang, Zhi-Hong Zhang, Ya-Wen Wang, Yu Peng
A dual mode fluorescent probe, which is based on an integration of fluorescein and coumarin fluorophores, was developed for the discrimination of Cys from Hcy and GSH. This probe (2) shows the advantage of quick reaction (5 min) with Cys, resulting in a strong fluorescence turn-on response when excited at 450 nm. Notably, it also demonstrates the ratiometric fluorescence property while excited by a shorter wavelength (332 nm). All of results suggest probe 2 has a high selectivity toward Cys even in the presence of other amino acids, cations and anions...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28206952/identification-of-sphingosine-kinase-1-sphk1-as-a-primary-target-of-icaritin-in-hepatocellular-carcinoma-cells
#5
Pei-Hua Lu, Min-Bin Chen, Yuan-Yuan Liu, Mian-Hua Wu, Wen-Ting Li, Mu-Xin Wei, Chao-Ying Liu, Shu-Kui Qin
Hepatocellular carcinoma (HCC) is a highly aggressive neoplasm. We aim to explore the anti-HCC activity by a natural prenylflavonoid icaritin. Icaritin was cytotoxic and pro-apoptotic when added to established (HepG2, KYN-2 and Huh-7 lines) and primary human HCC cells. At the signaling level, icaritin inhibited sphingosine kinase 1 (SphK1) activity in HCC cells, which led to pro-apoptotic ceramide production and JNK1 activation. SphK1 inhibition or silence (by shRNA/microRNA) mimicked icaritin-mediated cytotoxicity, and almost nullified icaritin's activity in HepG2 cells...
February 9, 2017: Oncotarget
https://www.readbyqxmd.com/read/28205610/cr-laao-an-l-amino-acid-oxidase-from-calloselasma-rhodostoma-venom-as-a-potential-tool-for-developing-novel-immunotherapeutic-strategies-against-cancer
#6
Tássia R Costa, Danilo L Menaldo, Karina F Zoccal, Sandra M Burin, Alexandre F Aissa, Fabíola A de Castro, Lúcia H Faccioli, Lusânia M Greggi Antunes, Suely V Sampaio
L-amino acid oxidases from snake venoms have been described to possess various biological functions. In this study, we investigated the inflammatory responses induced in vivo and in vitro by CR-LAAO, an L-amino acid oxidase isolated from Calloselasma rhodostoma venom, and its antitumor potential. CR-LAAO induced acute inflammatory responses in vivo, with recruitment of neutrophils and release of IL-6, IL-1β, LTB4 and PGE2. In vitro, IL-6 and IL-1β production by peritoneal macrophages stimulated with CR-LAAO was dependent of the activation of the Toll-like receptors TLR2 and TLR4...
February 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28203489/effects-of-anti-repulsive-guidance-molecule-c-rgmc-hemojuvelin-antibody-on-hepcidin-and-iron-in-mouse-liver-and-tumor-xenografts
#7
S V Torti, E Lemler, B K Mueller, A Popp, F M Torti
OBJECTIVE: Hepcidin is a peptide hormone produced by the liver that regulates systemic iron homeostasis. Hepcidin is also synthesized by tumors, where it contributes to tumor growth by increasing the tumoral retention of iron. Targeted reduction of hepcidin may therefore be useful in reducing tumor growth. H5F9-AM8 is an antibody in preclinical development for the anemia of chronic disease that reduces hepcidin synthesis by binding to RGMc, a co-receptor involved in the transcriptional induction of hepcidin by BMP6...
November 2016: Clinical & Experimental Pharmacology
https://www.readbyqxmd.com/read/28198103/synthesis-of-5%C3%AE-8%C3%AE-ergosterol-peroxide-3-carbamate-derivatives-and-fluorescent-mitochondria-targeting-conjugate-for-enhanced-anticancer-activities
#8
Ming Bu, Tingting Cao, Hongxia Li, Mingzhou Guo, Burton B Yang, Chengchu Zeng, Liming Hu
By inspiration of significant anticancer activity of our previously screened natural ergosterol peroxide (1), a series of novel ergosterol peroxide 3-carbamate derivatives were synthesized and characterized. The anti-proliferative activity of synthesized compounds against human hepatocellular carcinoma cells (HepG2, SK-Hep1) and human breast cancer cells (MCF-7, MDA-MB231) were investigated. Compound 3d, 3f and their hydrochloride exhibited significant in vitro anti-proliferative activity against the tested tumor cell lines, with IC50 values ranging from 0...
February 15, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28197966/signaling-pathway-involved-in-the-inhibitory-effect-of-fty720p-on-the-na-k-atpase-in-hepg2-cells
#9
Nadine Al Alam, Sawsan Ibrahim Kreydiyyeh
The Na(+)/K(+) ATPase modulates the activity of many transporters in the liver, and maintains the ionic constancy of the intracellular milieu, preserving thus normal functioning of hepatocytes. Previous work showed that FTY720P, a sphingosine one phosphate receptor agonist used in the treatment of multiple sclerosis, exerts in HepG2 cells, an inhibitory effect on the activity of the ATPase, mediated via PGE2. This study is an attempt to identify the signaling molecules involved downstream of the prostaglandin...
February 14, 2017: Journal of Cell Communication and Signaling
https://www.readbyqxmd.com/read/28197625/different-effect-of-resveratrol-to-induction-of-apoptosis-depending-on-the-type-of-human-cancer-cells
#10
Michinori Takashina, Sayaka Inoue, Kei Tomihara, Kengo Tomita, Kohshi Hattori, Qing-Li Zhao, Tokiko Suzuki, Makoto Noguchi, Wakana Ohashi, Yuichi Hattori
The effect of resveratrol on various human cancer cells was investigated with special focus on apoptotic cell death, in an attempt to further characterize its mechanism of action. There were great differences in the anti-viability effect of resveratrol between different types of human cancer cells. While the inhibition of cell viability by resveratrol was marked in U937 and MOLT-4 leukemia cells, resveratrol moderately inhibited cell viability in MCF-7 breast, HepG2 liver, and A549 lung cancer cells, and the effect was slight on cell viability in Caco-2, HCT116, and SW480 colon cancer cells...
January 24, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28197320/synthesis-biological-evaluation-and-autophagy-mechanism-of-12n-substituted-sophoridinamines-as-novel-anticancer-agents
#11
Chongwen Bi, Na Zhang, Peng Yang, Cheng Ye, Yanxiang Wang, Tianyun Fan, Rongguang Shao, Hongbin Deng, Danqing Song
A series of 12N-substituted sophoridinamine derivatives were synthesized and evaluated for their cytotoxic activities in human HepG2 hepatoma cells. Structure-activity relationship revealed that introduction of a suitable arylidene or arylethyl at the N'-end could greatly enhance antiproliferation potency. Among them, compound 6b possessing a N'-trimethoxyphenyl methylene exhibited potent antiproliferation effect against three human tumor cell lines including HepG2, leukemia (K562), and breast cancer (HMLE), with IC50 between 0...
February 9, 2017: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28197098/in-vitro-antiproliferative-and-apoptosis-inducing-effect-of-allium-atroviolaceum-bulb-extract-on-breast-cervical-and-liver-cancer-cells
#12
Somayeh Khazaei, Norhaizan M Esa, Vasudevan Ramachandran, Roslida A Hamid, Ashok K Pandurangan, Ali Etemad, Patimah Ismail
Natural products are considered potent sources for novel drug discovery and development. The multiple therapeutic effects of natural compounds in traditional medicine motivate us to evaluate the cytotoxic activity of bulb of Allium atroviolaceum in MCF7 and MDA-MB-231, HeLa and HepG2 cell lines. The bulb methanol extract of A. atroviolaceum was found to be an active cell proliferation inhibitor at the time and dose dependent manner. Determination of DNA content by flow cytometry demonstrated S and G2/M phase arrest of MCF-7 cell, correlated to Cdk1 downregulation, S phase arrest in MDA-MB-231 which is p53 and Cdk1-dependent, sub-G0 cell cycle arrest in HeLa aligned with Cdk1 downregulation, G0/G1, S, G2/M phase arrest in HepG2 which is p53-dependent...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28196955/identification-of-human-junctional-adhesion-molecule-1-as-a-functional-receptor-for-the-hom-1-calicivirus-on-human-cells
#13
Stanislav V Sosnovtsev, Carlos Sandoval-Jaime, Gabriel I Parra, Christine M Tin, Ronald W Jones, Jo Soden, Donna Barnes, Jim Freeth, Alvin W Smith, Kim Y Green
The Hom-1 vesivirus was reported in 1998 following the inadvertent transmission of the animal calicivirus San Miguel sea lion virus to a human host in a laboratory. We characterized the Hom-1 strain and investigated the mechanism by which human cells could be infected. An expression library of 3,559 human plasma membrane proteins was screened for reactivity with Hom-1 virus-like particles, and a single interacting protein, human junctional adhesion molecule 1 (hJAM1), was identified. Transient expression of hJAM1 conferred susceptibility to Hom-1 infection on nonpermissive Chinese hamster ovary (CHO) cells...
February 14, 2017: MBio
https://www.readbyqxmd.com/read/28194454/in-vitro-genotoxicity-of-carcinogenic-asarone-isomers
#14
S Haupenthal, K Berg, M Gründken, S Vallicotti, M Hemgesberg, K Sak, D Schrenk, M Esselen
The present study focused on genotoxic properties of the carcinogenic phenylpropanoids α-asarone and β-asarone, which are found in several herbs and spices, such as Acorus calamus or Acorus gramineus. Cytotoxic and genotoxic effects were determined in human liver carinoma HepG2 cells, in hamster lung fibroblast V79 cells and in human cytochrome P450 1A2 and human sulfotransferase 1C2 transfected V79 cells (tV79). The Alamar blue assay was used to measure cytotoxicity of both isomers prior to the identification of DNA damaging properties by single cell gel electrophoresis (comet assay)...
February 14, 2017: Food & Function
https://www.readbyqxmd.com/read/28194021/type-i-ifn-augments-il-27-dependent-trim25-expression-to-inhibit-hbv-replication
#15
Guangyun Tan, Qingfei Xiao, Hongxiao Song, Feng Ma, Fengchao Xu, Di Peng, Na Li, Xiaosong Wang, Junqi Niu, Pujun Gao, F Xiao-Feng Qin, Genhong Cheng
Hepatitis B virus (HBV) can cause chronic hepatitis B, which may lead to cirrhosis and liver cancer. Type I interferon (IFN) is an approved drug for the treatment of chronic hepatitis B. However, the fundamental mechanisms of antiviral action by type I IFN and the downstream signaling pathway are unclear. TRIM25 is an IFN-stimulated gene (ISG) that has an important role in RIG-I ubiquitination and activation. Whether TRIM25 is induced in liver cells by type I IFN to mediate anti-HBV function remains unclear...
February 13, 2017: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/28193566/in-vitro-evaluation-of-ruthenium-complexes-for-photodynamic-therapy
#16
Wenna Li, Qiang Xie, Linglin Lai, Zhentao Mo, Xiaofang Peng, Ennian Leng, Dandan Zhang, Hongxia Sun, Yiqi Li, Wenjie Mei, Shuying Gao
BACKGROUND: Photodynamic therapy (PDT) is a promising new anti-tumor treatment strategy. Photosensitizer is one of the most important components of PDT. In this work, the anticancer activities of PDTs mediated by six new ruthenium porphyrin complexes were screened. The mechanisms of the most efficacious candidate were investigated. METHODS: Photocytotoxicity of the six porphyrins was tested. The most promising complex, Rup-03, was further investigated using Geimsa staining, which indirectly detects reactive oxygen species (ROS) and subcellular localization...
February 10, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28193314/celecoxib-mediated-activation-of-endoplasmic-reticulum-stress-induces-de-novo-ceramide-biosynthesis-and-apoptosis-in-hepatoma-hepg2-cells
#17
Hyo Jin Maeng, Jae-Hwi Song, Goon-Tae Kim, Yoo-Jeong Song, Kangpa Lee, Jae-Young Kim, Tae-Sik Park
Ceramides are the major sphingolipid metabolites involved in cell survival and apoptosis. When HepG2 hepatoma cells were treated with celecoxib, the expression of the genes in de novo sphingolipid biosynthesis and sphingomyelinase pathway was upregulated and cellular ceramide was elevated. In addition, celecoxib induced endoplasmic reticulum (ER) stress in a time-dependent manner. SPTLC2, a subunit of serine palmitoyltransferase, was overexpressed by adenovirus. Adenoviral overexression of SPTLC2 (AdSPTLC2) decreased cell viability of HEK293 and HepG2 cells...
February 10, 2017: BMB Reports
https://www.readbyqxmd.com/read/28193229/involvement-of-fatty-acid-synthase-in-dengue-virus-infection
#18
Natthida Tongluan, Suwipa Ramphan, Phitchayapak Wintachai, Janthima Jaresitthikunchai, Sarawut Khongwichit, Nitwara Wikan, Supoth Rajakam, Sutee Yoksan, Nuttaporn Wongsiriroj, Sittiruk Roytrakul, Duncan R Smith
BACKGROUND: The mosquito transmitted Dengue virus (DENV) remains a significant public health problem in many tropical and subtropical countries. Increasing evidence has suggested that during the infection process cellular lipids play important roles at several stages of the replication cycle. This study sought to characterize the changes in lipid metabolism gene expression and investigated the role of one enzyme, fatty acid synthase, in DENV infection. METHODS: Transcriptional profiles of genes associated with lipid metabolism were evaluated by real-time PCR after infection of different cell lines (HepG2 and HEK293T/17) and with different DENVs (laboratory adapted and low passage)...
February 13, 2017: Virology Journal
https://www.readbyqxmd.com/read/28193057/development-of-aptes-decorated-hepg2-cancer-stem-cell-membrane-chromatography-for-screening-active-components-from-salvia-miltiorrhiza
#19
Xuan Ding, Yan Cao, Yongfang Yuan, Zhirong Gong, Yue Liu, Liang Zhao, Lei Lv, Guoqing Zhang, Dongyao Wang, Dan Jia, Zhenyu Zhu, Zhanying Hong, Xiaofei Chen, Yifeng Chai
Cell membrane chromatography (CMC) is an ideal method for screening potential active components acting on target cell membranes from a complex system, such as herbal medicines. But due to the decay and falling-off of membranes, the CMC column suffers from short life span and low reproducibility. This has greatly limited the application of this model, especially when the cell materials are hard to obtain. To solve this problem, a novel type of (3-aminopropyl)triethoxysilane (APTES)-decorated silica gel was employed...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28193024/evaluating-tumor-associated-activity-of-extracellular-sulfatase-by-analyzing-naturally-occurring-substrate-in-tumor-microenvironment-of-hepatocellular-carcinoma
#20
Yue Yu, Hao Li, Yucai Yang, Yitao Ding, Zhaoxia Wang, Genxi Li
The progress of cancer is intimately connected with the activity of the extracellular matrix (ECM) enzymes. To evaluate the promoting effect of these enzymes on tumor development in a pathological biocontext, we propose in this work to analyze their natural substrates in the ECM. This strategy is demonstrated by studying heparan sulfate (HS), the substrate of ECM sulfatase, in the development of hepatocellular carcinoma (HCC). An assay is designed to study the abundance and sulfation of HS and to evaluate the interactions between HS and the growth factors, such as fibroblast growth factor 2 (FGF2)...
December 20, 2016: Analytical Chemistry
keyword
keyword
100525
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"