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anti-BP IgE

Ariadne Hadjikyriacou Saniklidou, Patrick J Tighe, Lucy C Fairclough, Ian Todd
Bullous pemphigoid (BP) is the most common autoimmune skin disease of blistering character. The underlying pathophysiological mechanism involves an immune attack, usually by IgG class autoantibodies, on the autoantigen BP 180/BPAg2, which is a type XVII collagen (COL17) protein acting as the adhesion molecule between the epidermis and the basement membrane of the dermis. About 40 years ago, following consistent findings of elevated total serum IgE levels in BP patients, it was hypothesized that IgE may be involved in the pathophysiology of BP...
October 25, 2017: Archives of Dermatological Research
Nina van Beek, Nadine Lüttmann, Franziska Huebner, Andreas Recke, Ingolf Karl, Franziska Sophie Schulze, Detlef Zillikens, Enno Schmidt
Importance: Bullous pemphigoid (BP) is by far the most frequent autoimmune blistering disease. The presence of IgE autoantibodies against the transmembrane protein BP antigen 2 (BP180, type XVII collagen) has previously been reported in 22% to 100% of BP serum samples, and the pathogenic relevance of anti-BP180 IgE has been suggested in various experimental models and by the successful use of omalizumab in individual patients with BP. Objectives: To determine the rate of anti-BP180-reactive IgE in BP, to evaluate the diagnostic relevance of anti-BP180 IgE in BP, and to correlate anti-BP180 IgE with disease activity and the clinical phenotype of patients with BP...
January 1, 2017: JAMA Dermatology
T Hashimoto, A Ohzono, K Teye, S Numata, S Hiroyasu, D Tsuruta, T Hachiya, K Kuroda, M Hashiguchi, T Kawakami, N Ishii
BACKGROUND: IgE autoantibodies are considered to be involved in the pathogenesis of bullous pemphigoid (BP), particularly inflammatory and erythematous phenotypes. OBJECTIVES: To develop reliable enzyme-linked immunosorbent assays (ELISAs) for the detection of IgE autoantibodies to both BP180 and BP230 in BP sera, and to compare the ELISA results with clinical features. METHODS: We used commercially available IgG ELISAs to develop IgE ELISAs for both BP180 and BP230...
July 2017: British Journal of Dermatology
G Balakirski, A Alkhateeb, H F Merk, M Leverkus, M Megahed
BACKGROUND: Bullous pemphigoid (BP) is an autoimmune blistering disease that is characterized by formation of subepidermal bullae due to functional disturbance of the hemidesmosomal proteins on the keratinocytes at the basal membrane zone. In recent years, several studies have emphasized the important role of IgE autoantibodies in the pathogenesis of BP. Consequently, a therapeutic approach using IgE depleting antibodies, such as a humanized monoclonal anti-IgE antibody (e.g. omalizumab) may represent a new option for treatment of this autoimmune disease...
October 2016: Journal of the European Academy of Dermatology and Venereology: JEADV
Angelika Wagner, Irma Schabussova, Mirjana Drinic, Johnnie Akgün, Gerhard Loupal, Michael Kundi, Anja Joachim, Ursula Wiedermann
INTRODUCTION: Previously, we have shown that oral infection with Toxoplasma gondii oocysts prevented type I allergy in mice. Here we investigated whether the application of a T. gondii oocyst lysate antigen (OLA) could also reduce allergy development. BALB/c mice were immunised twice with OLA followed by sensitisation with the major birch pollen (BP) allergen Bet v 1 and an aerosol challenge with BP extract. METHODS: First, we tested OLA in vitro. Stimulation of splenocytes and bone marrow-derived dendritic cells (BMDC) with OLA led to the production of pro-inflammatory and regulatory cytokines such as IL-6, IFN-γ and IL-10...
2016: PloS One
Huihui Xing, Hui Shao, Xiuqin Cao, Zhiwei Yang
OBJECTIVE: To induce the expression of human soluble Fc epsilon receptor I alpha (sFcepsilonR1α) in a prokaryotic expression vector, purify the recombinant human sFcepsilonR1α protein, detect its binding affinity for human serum IgE antibodies and detect the levels of sFcepsilonR1α, sFcepsilonR1α-IgE and FcepsilonR1α antibodies. METHODS: The FcepsilonR1α extracellular region gene was amplified using nested polymerase chain reaction (PCR) and was expressed in a prokaryotic expression vector pET-sFcepsilonR1α using recombinant DNA technology under optimal conditions...
May 2016: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Liu Bing, Zhou Xiping, Li Li, Peng Jun, Wang Yi-Xia, Yang Min, Liu Qing, Sun Qiu-Ning, Jin Hong-Zhong, Zuo Ya-Gang
Bullous pemphigoid (BP), a common autoimmune skin disease, is associated with IgG autoantibodies against the hemidesmosomal proteins, BP180 and BP230. In addition to IgG, IgE has been shown to play a role in the disease. However, the association between disease activity and IgE specific to the NC16A domain of BP180 in blister fluid remains unclear. Our objective was to evaluate the correlation between BP disease activity and BP180 NC16A-specific IgE sera and blister fluid titers, and to analyze changes during treatment...
November 2015: Archives of Dermatological Research
Hideyuki Ujiie
Bullous pemphigoid (BP) is a common autoimmune blistering skin disease in which two hemidesmosomal components--the transmembrane collagen XVII (BP180 or BPAG2) and the plakin family protein BP230 (BPAG1)--are targeted by autoimmunity. Of these, collagen XVII (COL17) is thought to be a major autoantigen, and vital roles of IgG autoantibodies in blister formation have been elucidated. However, BP shows distinct features, including pruritic urticarial erythema and eosinophilic infiltration, which may be independent of IgG-mediated autoimmunity...
April 2015: Journal of Dermatological Science
Monika Kalowska, Olga Ciepiela, Cezary Kowalewski, Urszula Demkow, Robert A Schwartz, Katarzyna Wozniak
Bullous pemphigoid (BP) is characterized by IgG and IgE autoantibodies to the NC16a domain of BP180. This study evaluated the correlation between body surface area (BSA), total serum IgE and enzyme-linked immunoassay (ELISA) for IgG and IgE anti-NC16a in 77 patients with BP in the active stage, and the degree of conversion to negative of the studied parameters in clinical remission. Statistically positive correlations were observed between BSA and examined parameters (correlation index 0.2548, 0.2491, 0.311, respectively)...
February 2016: Acta Dermato-venereologica
G Balakirski, H F Merk, M Megahed
BACKGROUND: Bullous pemphigoid is an autoimmune blistering disease that is associated with appearance of subepidermal blisters. IgG antibodies against components of the epithelial basement membrane (BP 180 and 230 antigens) can be typically found in serum of patients. Direct immunofluorescence reveals usually a linear deposition of IgG and/or C3 along the basement membrane, but other immunoglobulins may also be present. CASE PRESENTATION: Our patient had no detectable formation of tense blisters of his skin; instead, the clinical picture was rather compatible with a prurigo simplex subacuta or a pruritic variant of atopic dermatitis...
December 2014: Der Hautarzt; Zeitschrift Für Dermatologie, Venerologie, und Verwandte Gebiete
Ayşe Akman-Karakaş, Arzu Didem Yalcin, Saliha Koç, Saadet Gumuslu, Erkan Ergun, Gizem Esra Genc, Gozde Ongut, Soner Uzun, Erkan Alpsoy
BACKGROUND: CD200 is a novel immunosuppressive molecule, existing both as cell membrane bound and as a soluble form in serum (sCD200), which acts to regulate inflammatory and acquired immune responses. Recently, our study group showed that sCD200 was found in serum and blister fluid in a patient with bullous pemphigoid and that anti-IgE therapy impacted those levels. We therefore planned this study to evaluate the soluble serum CD200 levels of bullous pemphigoid patients and compare it with that of healthy controls...
2014: Clinical Laboratory
Kenneth K Yu, Ashley B Crew, Kelly A N Messingham, Janet A Fairley, David T Woodley
BACKGROUND: Bullous pemphigoid (BP) responds to a variety of immunosuppressive agents and usually controls, but does not cure, the disease. Omalizumab, Food and Drug Administration-approved for asthma, selectively suppresses the activity of IgE, an important immunoglobulin in the pathogenesis of BP. OBJECTIVE: We wished to determine if systemic omalizumab would have a therapeutic effect in patients with BP. METHODS: We treated 6 patients with BP using omalizumab and followed up their disease for up to 42 months...
September 2014: Journal of the American Academy of Dermatology
K A N Messingham, H M Holahan, J A Fairley
Bullous pemphigoid (BP), a cutaneous autoimmune blistering disease, has provided a useful model to elucidate a role for IgE in autoimmunity. IgE antibodies specific for the BP180 autoantigen are detected in sera and biopsy samples from the majority of BP patients. In BP biopsies, both IgE and BP180 antigen localize to the surface of mast cells, and incubation of circulating basophils from these patients with BP180 protein triggered degranulation. The in vivo pathogenicity of BP180-specific IgE was confirmed in mouse models, where injection of purified BP IgE into human skin grafted onto nu/nu mice replicated the early phase of lesion development, including mast cell degranulation, eosinophil infiltration and development of urticarial plaques...
August 2014: Immunologic Research
P Xavier-Elsas, C L C A Silva, L Pinto, T Queto, B M Vieira, M G Aranha, B De Luca, D Masid-de-Brito, R A Luz, R S Lopes, R Ferreira, M I Gaspar-Elsas
Allergic airway inflammation is attenuated by oral tolerization (oral exposure to allergen, followed by conventional sensitization and challenge with homologous antigen), which decreases airway allergen challenge-induced eosinophilic infiltration of the lungs and bone marrow eosinophilia. We examined its effects on bone marrow eosinophil and neutrophil production. Mice of wild type (BP-2, BALB/c, and C57BL/6) and mutant strains (lacking iNOS or CD95L) were given ovalbumin (OVA) or water (vehicle) orally and subsequently sensitized and challenged with OVA (OVA/OVA/OVA and H2O/OVA/OVA groups, resp...
2013: BioMed Research International
Bing Liu, Ya-gang Zuo, Xi-ping Zhou, Chun-xia He, Jun Li, Duerna Tie, Li Li, Qiu-ning Sun
OBJECTIVE: To establish a method of detecting circulating immunoglobulin E (IgE) autoantibodies for BP180NC16A and evaluate its significance in bullous pemphigoid (BP). METHODS: GST-NC16A fusion proteins were expressed in Escherichia coli using the pGEX-2T expression system and purified by glutathione affinity chromatography.For optimal working conditions of enzyme-linked immunoabsorbent assay (ELISA), checkerboard titrations were performed with serial dilutions of antigen...
July 23, 2013: Zhonghua Yi Xue za Zhi [Chinese medical journal]
Yong-xia Zhang, Bao-cheng Wang, Xin Yu, Yun-jian Dai, Yong-zhi He, Cong Cong, Yong Xia, Ming-rong Wang
Total mRNA was extracted from lymphocytes separated from the peripheral blood of allergic patients, and then variable region of heavy chain (VH) and variable region of light chain (VL) cDNA library were constructed by RT-PCR. Human scFv templates for rabbit reticulocyte lysate ribosome display were assembled by primers and linker peptide (Gly4Ser)3. mRNA bound in antibody-ribosome-mRNA complexes was recovered using in-situ single primer RT-PCR, and three rounds of anti-IgE scFv DNA were enriched. The target DNA fragments were double enzyme digested and ligated into plasmid pET22b (+), followed by transformation in E...
October 2012: Yao Xue Xue Bao, Acta Pharmaceutica Sinica
Hai-Wang Shen, Min-Jie Cao, Qiu-Feng Cai, Mi-Mi Ruan, Hai-Yan Mao, Wen-Jin Su, Guang-Ming Liu
Arginine kinase (AK) is an important enzyme participating in energy metabolism in invertebrates, but, to date, there have been no reports that AK from octopus is an allergen. In this study, octopus AK was purified, and its molecular biological, immunological, and physicochemical characterizations were analyzed. The results showed that octopus AK was purified and confirmed by mass spectrometry for the first time, and its molecular mass was 38 kDa. The full-length gene sequence of octopus AK encompassed 1209 bp and was predicted to encode a protein with 348 amino acid residues...
March 7, 2012: Journal of Agricultural and Food Chemistry
Elliott C Lasser
A long history of searching for the etiology of X-ray contrast material (CM) reactions has led to the understanding that the CM do not produce anti-CM antigens. Since CM reactions are anaphylactoid in nature, however, a source for mast cell activation was sought. This resulted in the finding that concentrated CM could suppress mast cell activation by attachment to the Fc portion of IgE and IgG. This is presumed to be a steric hindrance effect. In a study of the effects of CM on BP and a study of the effects of CM in sensitized rats, it was concluded that less concentrated CM activated mast cells and that this mechanism was best explained by bridging of adjacent IgE molecules via attachment to their Fc segments...
2011: Journal of Allergy
Lei Wan, Jiun-Bo Chen, Hsih Hsin Chen, Janice Huang, Hui-Ming Yu, Shue-Fen Luo, Fuu Jen Tsai, Tse Wen Chang
The epsilon chain of membrane-bound IgE (mIgE) is expressed predominantly as a "long" isoform, containing an extra segment of 52 amino acid (a.a.) residues, referred to as C epsilon mX, between the CH4 domain and the C-terminal membrane-anchoring transmembrane peptide. C epsilon mX results from an alternative splicing of the epsilon RNA transcript at 156-bp upstream of the splicing acceptor site used by the "short" isoform. Here, based on an analysis of the C epsilon mX genomic DNA sequences of 320 subjects residing in Taiwan, we report that single-nucleotide polymorphisms have been found at two positions, namely, G/T at #46 and A/G at #93 (along the 156 bp of C epsilon mX), with the former creating an amino acid change from Val to Leu at #16 (along the 52 a...
May 2010: Immunogenetics
Kelly A N Messingham, Megan H Noe, Marisa A Chapman, George J Giudice, Janet A Fairley
Bullous pemphigoid (BP) is a humoral autoimmune disease directed predominantly against the non-collagenous NC16A domain of the BP180 hemidesmosomal protein. Our laboratory has recently shown, using a mouse xenograft model, that passive transfer of IgE autoantibodies from BP sera induces a skin phenotype that recapitulates the early phases of the disease. Herein, we describe the development of a highly specific and sensitive ELISA to detect circulating IgE autoantibodies that recognize BP180-NC16A. Using this assay, we detected NC16A-specific IgE-class autoantibodies in 77% of BP sera...
July 31, 2009: Journal of Immunological Methods
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