keyword
https://read.qxmd.com/read/38486799/light-sensitive-peg-hydrogel-with-antibacterial-performance-for-pacemaker-pocket-infection-prevention
#21
JOURNAL ARTICLE
Yurong Xiong, Qingyun Zhang, Juan Li, Nan Zhang, Xiaoshu Cheng, Quanbin Dong, Huihui Bao
Prevention of cardiovascular implantable electronic devices (CIED) infection is crucial for successful outcomes. In this study, we report an adhesive and antibacterial hydrogel coating for CIED infection treatment, by immobilizing polyethylene glycol (PEG) and 2'- O -hydroxypropyl trimethyl ammonium chloride chitosan (HAC) on Ti surface. Initial alkali and APTES treatment caused the formation of -NH2 to enhance the adhesion of the hydrogel coating to Ti implants, followed by immobilizing a photo-cross-linkable PEG/2'- O -HTACCS hydrogel on Ti/OH/NH2 surface...
April 2024: Materials today. Bio
https://read.qxmd.com/read/38486784/tracking-enteric-pathogen-contamination-from-on-site-sanitation-facilities-to-groundwater-in-selected-rural-areas-of-vhembe-district%C3%A2-municipality-limpopo-province-south-africa
#22
JOURNAL ARTICLE
Jeridah Matlhokha Sekgobela, Colette Mmapenya Khabo-Mmekoa, Maggy Ndombo Benteke Momba
Groundwater is valued as a source of potable water, although it is vulnerable to environmental pollution. The aim of this study was to track enteric pathogen contamination from on-site sanitation (OSS) facilities to 70 household boreholes used by four villages of the Vhembe District Municipality. Two objectives were pursued: to measure the lateral distance between the borehole and the sanitation facilities in household yards, and to track the enteric pathogens. The borehole abstraction point and OSS system distance were determined using a steel measuring tape...
March 15, 2024: Heliyon
https://read.qxmd.com/read/38486475/a-novel-immunoinformatics-approach-for-developing-a-poly-epitope-vaccine-targeting-foot-and-mouth-disease-virus-exploiting-structural-vp-proteins
#23
JOURNAL ARTICLE
Muhammad Saad Bhutta, Muhammad Awais, Sahar Sadaqat, Fernando Berton Zanchi, Naila Shahid, Abdul Qayyum Rao
Foot and mouth Disease virus (FMDV) belongs to Picornaviridae family and Aphthovirus genus causing Foot and mouth disease (FMD) in cloven-hoofed animals. FMDV, a prevalent virus induces both acute and chronic infections with high mutation rates resulting in seven primary serotypes, making vaccine development indispensable. Due to time and cost effectiveness of the immunoinformatic approach, we designed in-silico polyepitope vaccine (PEV) for the curtailment of FMDV. Structural and immunogenic parts of FMDV (Viral Protein 1 (VP1), Viral Protein 2 (VP2), Viral Protein 3 (VP3), and Viral Protein 4 (VP4)) were used to design the cytotoxic T Lymphocyte (CTL), Helper T Lymphocyte (HTL), and B-cell epitopes, followed by screening for antigenic, non-allergenic, Interferon (IFN) simulator, and non-toxicity, which narrowed down to 7 CTL, 3 HTL, and 12 B-cell epitopes...
March 15, 2024: Journal of Biomolecular Structure & Dynamics
https://read.qxmd.com/read/38486355/enhancing-a-multi-purpose-artificial-urine-for-culture-and-gene-expression-studies-of-uropathogenic-escherichia-coli-strains
#24
JOURNAL ARTICLE
Patricia T Rimbi, Nicky O'Boyle, Gillian R Douce, Mariagrazia Pizza, Roberto Rosini, Andrew J Roe
AIMS: The main objective of this study was to modify a recently reported multi-purpose artificial urine (MP-AU) for culture and gene expression studies of uropathogenic Escherichia coli (UPEC) strains. METHODS AND RESULTS: We used liquid chromatography mass spectrometry (LC-MS) to identify and adjust the metabolic profile of MP-AU closer to that of pooled human urine (PHU). Modification in this way facilitated growth of UPEC strains with growth rates similar to those obtained in PHU...
March 14, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38486350/smelly-shark-smelly-ray-what-is-infecting-you
#25
JOURNAL ARTICLE
Maíra Duarte Cardoso, Oswaldo Luiz de C Maciel, André Luiz Medeiros de Souza, Emily Moraes Roges, Verônica Dias Gonçalves, Salvatore Siciliano, Dália Dos Prazeres Rodrigues, Rachel Ann Hauser-Davis
AIMS: Although elasmobranchs are consumed worldwide, bacteriological assessments for this group are still sorely lacking. In this context, this study assessed bacteria of sharks and rays from one of the most important landing ports along the Rio de Janeiro coast. METHODS AND RESULTS: Bacteria were isolated from the cloacal swabs of the sampled elasmobranchs. They were cultured and Vibrio, Aeromonas and Enterobacterales were isolated and identified. The isolated bacteria were then biochemically identified and antimicrobial susceptibility assays were performed...
March 14, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38486187/phytoconstituent-analysis-anti-inflammatory-antimicrobial-and-anticancer-effects-of-nano-encapsulated-convolvulus-arvensis-l-extracts
#26
JOURNAL ARTICLE
Ezzat E A Osman, Mohamed A Shemis, El-Sayed S Abdel-Hameed, Abdullah E Gouda, Hanem Hassan, Nahla Atef, Samah Mamdouh
BACKGROUND: The Convolvulus genus is distributed all over the world and has a long history in traditional medicine. As nanotechnology expands its reach into areas like drug delivery and biomedicine, this study intends to assess the potential of Convolvulus arvensis L. extracts as anti-bacterial, anti-inflammatory and anti-cancer agents, along with chemical profiling of the methanolic (MeOH) extract active ingredients. METHODS: The chemical composition of an 85% MeOH extract was investigated by liquid chromatography with an electrospray source connected to mass spectrometry (LC-ESI-MS)...
March 14, 2024: BMC complementary medicine and therapies
https://read.qxmd.com/read/38486152/isolation-and-characterization-of-lytic-bacteriophages-from-various-sources-in-addis-ababa-against-antimicrobial-resistant-diarrheagenic-escherichia-coli-strains-and-evaluation-of-their-therapeutic-potential
#27
JOURNAL ARTICLE
Tamirat Salile Sada, Tesfaye Sisay Tessema
BACKGROUND: Escherichia coli is a common fecal coliform, facultative aerobic, gram-negative bacterium. Pathogenic strains of such microbes have evolved to cause diarrhea, urinary tract infections, and septicemias. The emergence of antibiotic resistance urged the identification of an alternative strategy. The use of lytic bacteriophages against the control of pathogenic E. coli in clinics and different environmental setups (waste and drink water management) has become an alternative therapy to antibiotic therapy...
March 14, 2024: BMC Infectious Diseases
https://read.qxmd.com/read/38485761/characterisation-of-colistin-resistance-in-gram-negative-microbiota-of-pregnant-women-and-neonates-in-nigeria
#28
JOURNAL ARTICLE
E A R Portal, K Sands, C Farley, I Boostrom, E Jones, M Barrell, M J Carvalho, R Milton, K Iregbu, F Modibbo, S Uwaezuoke, C Akpulu, L Audu, C Edwin, A H Yusuf, A Adeleye, A S Mukkadas, D Maduekwe, S Gambo, J Sani, T R Walsh, O B Spiller
A mobile colistin resistance gene mcr was first reported in 2016 in China and has since been found with increasing prevalence across South-East Asia. Here we survey the presence of mcr genes in 4907 rectal swabs from mothers and neonates from three hospital sites across Nigeria; a country with limited availability or history of colistin use clinically. Forty mother and seven neonatal swabs carried mcr genes in a range of bacterial species: 46 Enterobacter spp. and single isolates of; Shigella, E. coli and Klebsiella quasipneumoniae...
March 14, 2024: Nature Communications
https://read.qxmd.com/read/38485701/substrate-specificity-of-mycobacterium-tuberculosis-trna-terminal-nucleotidyltransferase-toxin-ment3
#29
JOURNAL ARTICLE
Jun Liu, Yuka Yashiro, Yuriko Sakaguchi, Tsutomu Suzuki, Kozo Tomita
Mycobacterium tuberculosis transfer RNA (tRNA) terminal nucleotidyltransferase toxin, MenT3, incorporates nucleotides at the 3'-CCA end of tRNAs, blocking their aminoacylation and inhibiting protein synthesis. Here, we show that MenT3 most effectively adds CMPs to the 3'-CCA end of tRNA. The crystal structure of MenT3 in complex with CTP reveals a CTP-specific nucleotide-binding pocket. The 4-NH2 and the N3 and O2 atoms of cytosine in CTP form hydrogen bonds with the main-chain carbonyl oxygen of P120 and the side chain of R238, respectively...
March 15, 2024: Nucleic Acids Research
https://read.qxmd.com/read/38485657/risk-factors-and-outcomes-of-bloodstream-infection-from-a-urinary-source-in-kidney-transplant-recipients
#30
JOURNAL ARTICLE
Emily M Eichenberger, Maja Wichhart Donzo, Rebecca Anderson, Geeta Karadkhele, Stephanie M Pouch, Christian P Larsen
BACKGROUND: Bacteriuria is common among kidney transplant recipients (KTR). Risk factors and outcomes associated with bloodstream infection due to a urinary source (BSIU) in KTR are poorly understood. METHODS: This single center case-control study from 2010 to 2022 compared KTR with BSIU to those with bacteria without bloodstream infection (BU). Multivariable logistic regression identified BSIU risk factors, and Cox models assessed its impact on graft failure. RESULTS: Among 3435 patients, who underwent kidney transplantation at Emory Hospital, 757 (22%) developed bacteriuria, among whom 142 (18...
March 2024: Clinical Transplantation
https://read.qxmd.com/read/38485092/cell-free-synthesis-of-the-salmonella-specific-broad-host-range-bacteriophage-felixo1
#31
JOURNAL ARTICLE
John A McFarlane, David Garenne, Vincent Noireaux, Steven D Bowden
Phage-based biocontrol of foodborne Salmonella is limited by the requisite use of Salmonella to propagate the phages. This limitation can be circumvented by producing Salmonella phages using a cell-free gene expression system (CFE) with a non-pathogenic chassis. Here, we produce the Salmonella phage felixO1 using an E. coli-based CFE system.
March 12, 2024: Journal of Microbiological Methods
https://read.qxmd.com/read/38485027/deciphering-the-behavior-and-potential-mechanism-of-biochar-at-different-pyrolysis-temperatures-to-alleviate-membrane-biofouling
#32
JOURNAL ARTICLE
Xiaohuan Yang, Silian Xia, Litu Hao, Duanyun Tian, Lianxu Wang, Rong Chen
Biofouling limits applications of membrane technology in wastewater treatment, but dosing additives to membrane tanks is an effective method to alleviate biofouling. In this study, biochar derived from corncob and pyrolyzed at 300, 500, and 700 °C was dosed to determine the underlying anti-biofouling mechanism. The effects of the biochar on the membrane properties and foulant behavior were systematically investigated. The results showed that biochar delayed the occurrence of the fouling transition (0...
March 12, 2024: Science of the Total Environment
https://read.qxmd.com/read/38485022/antibacterial-efficacy-mode-of-action-and-safety-of-a-novel-nano-antibiotic-against-antibiotic-resistant-escherichia-coli-strains
#33
JOURNAL ARTICLE
Chukwudi S Ubah, Lok R Pokhrel, Jordan E Williams, Shaw M Akula, Stephanie L Richards, Gregory D Kearney, Andre Williams
Globally rising antibiotic-resistant (AR) and multi-drug resistant (MDR) bacterial infections are of public health concern due to treatment failure with current antibiotics. Enterobacteria, particularly Escherichia coli, cause infections of surgical wound, bloodstream, and urinary tract, including pneumonia and sepsis. Herein, we tested in vitro antibacterial efficacy, mode of action (MoA), and safety of novel amino-functionalized silver nanoparticles (NH2 -AgNP) against the AR bacteria. Two AR E. coli strains (i...
March 12, 2024: Science of the Total Environment
https://read.qxmd.com/read/38484844/isolation-of-shiga-toxin-producing-escherichia-coli-from-the-surfaces-of-beef-carcasses-in-slaughterhouses-in-japan
#34
JOURNAL ARTICLE
Shunsuke Ikeuchi, Shouhei Hirose, Kohei Shimada, Ayako Koyama, Shoji Ishida, Naoto Katayama, Takehiko Suzuki, Akiko Tokairin, Mayumi Tsukamoto, Yuki Tsue, Kenichi Yamaguchi, Hideo Osako, Sachiko Hiwatashi, Yumi Chiba, Hiroshi Akiyama, Hideki Hayashidani, Yukiko Hara-Kudo
Shiga toxin-producing E. coli (STEC) is an important foodborne pathogen worldwide. It is necessary to control and prevent STEC contamination on beef carcasses in slaughterhouses because STEC infection is associated with beef consumption. However, the frequencies of STEC contamination of beef carcasses in various slaughterhouses in Japan are not well known. Herein, we investigated the contamination of beef carcasses with STEC in slaughterhouses to assess the potential risks of STEC. In total, 524 gauze samples were collected from the surfaces of beef carcasses at 12 domestic slaughterhouses from November 2020 to February 2023...
March 12, 2024: Journal of Food Protection
https://read.qxmd.com/read/38484816/flexible-cellulose-nanofibers-mxene-composite-films-for-uv-shielding-packaging
#35
JOURNAL ARTICLE
Meng He, Yujia Huang, Xinjiang Zhang, Wenyu Zhu, Wenjing Shao, Jinhua Wang, Dingfeng Xu, Wei Yao
Cellulose nanofibers (CNF) based films are promising packaging materials, but the lack of special functions (especially UV-shielding property) usually restrict their further applications. In this work, MXene was incorporated into the CNF film by a direct solvent volatilization induced film forming method to study its UV-shielding property for the first time, which avoided the using of a vacuum filtration equipment. The composite films containing glycerin could be folded repeatedly without breaking, showing good flexibility...
March 12, 2024: International Journal of Biological Macromolecules
https://read.qxmd.com/read/38484800/resolving-the-polycistronic-aftermath-essential-role-of-topoisomerase-ia-in-preventing-r-loops-in-leishmania
#36
JOURNAL ARTICLE
Payel Das, Arnab Hazra, Saradindu Saha, Sadhana Roy, Mandrita Mukherjee, Saugata Hazra, H K Majumdar, Somdeb BoseDasgupta
Kinetoplastid parasites are 'living bridges' in the evolution from prokaryotes to higher eukaryotes. The near-intronless genome of the kinetoplastid Leishmania, exhibit polycistronic transcription which can facilitate R-loop formation. Therefore, to prevent such DNA-RNA hybrids Leishmania has retained prokaryotic-like DNA Topoisomerase IA (LdTOPIA) in course of evolution. LdTOPIA is an essential enzyme which is expressed ubiquitously and is adapted for the compartmentalized eukaryotic form in harboring functional bipartite nuclear localization signals...
March 12, 2024: Journal of Biological Chemistry
https://read.qxmd.com/read/38484709/invade-to-evade-e-%C3%A2-coli-s-gutsy-survival-strategies
#37
JOURNAL ARTICLE
Yuval Mulla, Tobias Bollenbach
Antibiotic resistance is often studied in vitro, limiting the understanding of in vivo mechanisms that affect antibiotic treatment. In this issue of Cell Host & Microbe, Rodrigues et al. show that specific mutations allow bacteria to invade intestinal cells in a mouse model, thereby evading antibiotic treatment.
March 13, 2024: Cell Host & Microbe
https://read.qxmd.com/read/38484499/quantum-dots-qds-attached-magnetic-beads-mbs-for-on-chip-efficient-capture-and-detection-of-bacteria-in-ready-to-eat-rte-foods
#38
JOURNAL ARTICLE
Yunxing Li, Yihong Xu, Winnie C Soko, Hongyan Bi
In this study, we established a versatile and simple magnetic-assisted microfluidic method for fast bacterial detection. Quantum dots (QDs) were loaded onto magnetic beads (MBs) to construct performance enhanced on-chip capture of bacteria. Escherichia coli (E. coli), as a model bacterium was studied. CdSe QDs were deposited onto the surface of Fe3 O4 MBs through layer-by-layer self-assembly to enhance the loading of antibodies (Abs). MBs functionalized with anti-E. coli antibody molecules in a micropillar-based microfluidic chip were utilized to capture E...
March 8, 2024: Talanta
https://read.qxmd.com/read/38484476/rapid-diagnostic-of-multidrug-resistant-sepsis-pathogens-directly-from-blood-culture-bottles-using-maldi-tof-and-the-eucast-rast
#39
JOURNAL ARTICLE
Jesus G M Pariona, Flavio A Oliveira, Patrícia Helena Scoton, Heli J Barrón-Pastor, Eva M M Pariona, Tania R Zaccariotto, Nilton Lincopan, Carlos E Levy
In this study, rapid diagnostic of multidrug-resistant (MDR) sepsis pathogens, directly from positive blood culture (BC) bottles, was evaluated by combining MALDI-TOF and the EUCAST Rapid Antimicrobial Susceptibility Testing (RAST). Carbapenemase production in Escherichia coli and Klebsiella pneumoniae isolates was also evaluated by RAST. From 171 positive BC bottles analyzed, 79 (46 %) MDR species, including E. coli (4/34, 12 %), K. pneumoniae (33/48, 69 %), Pseudomonas aeruginosa (12/12, 100 %), Acinetobacter baumannii (15/15, 100 %), and Staphylococcus aureus (14/37, 38 %) displaying resistance to beta-lactams, fluoroquinolones, aminoglycosides, and/or trimethoprim/sulphamethoxazole, were identified...
March 8, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38484349/molecular-analysis-of-multidrug-resistant-e-coli-in-pediatric-utis-findings-from-a-nigerian-hospital
#40
JOURNAL ARTICLE
Eunice Damilola Wilkie, Chidinma Vivian Ezeani, Deborah Oluwasola Olasehinde, Omolanke Temitope Oyedemi, Jude Oluwapelumi Alao, Anthonia Olufunke Oluduro, Oluwapelumi Breakthrough Oyetunde
INTRODUCTION: This study aimed to isolate and characterize antibiotic-resistant Escherichia coli from urine samples of children at the Mother and Child Hospital in Ondo State, Nigeria, assessing antibiogram profiling and resistance genes. METHODOLOGY: Three hundred urine samples (158 females, 142 males), aged 3-5 years, were collected, transported on ice, and analyzed bacteriologically. E. coli and Gram-negative bacteria were isolated using Eosin Methylene Blue agar and identified through colony morphology and biochemical tests...
February 29, 2024: Journal of Infection in Developing Countries
keyword
keyword
100494
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.