keyword
https://read.qxmd.com/read/38493438/development-of-a-sensor-for-disulfide-bond-formation-in-diverse-bacteria
#1
JOURNAL ARTICLE
Dyotima, Sally Abulaila, Jocelyne Mendoza, Cristina Landeta
UNLABELLED: In bacteria, disulfide bonds contribute to the folding and stability of proteins important for processes in the cellular envelope. In Escherichia coli , disulfide bond formation is catalyzed by DsbA and DsbB enzymes. DsbA is a periplasmic protein that catalyzes disulfide bond formation in substrate proteins, while DsbB is an inner membrane protein that transfers electrons from DsbA to quinones, thereby regenerating the DsbA active state. Actinobacteria including mycobacteria use an alternative enzyme named VKOR, which performs the same function as DsbB...
March 13, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38492832/aristolochia-bracteolata-flower-extract-based-phytosynthesis-and-characterization-of-agnps-antimicrobial-antidiabetic-and-antioxidant-activities-potential-assessment
#2
JOURNAL ARTICLE
Anh-Tuan Le, Hai-Anh Ha, Mysoon Al-Ansari, Kasber Elankathirselvan, Latifah A Al-Humaid
The study was carried out to evaluate the effectiveness of the Aristolochia bracteolata water flower extract-mediated AgNPs synthesis and assess their antimicrobial potential. According to the experimental and analytical results, A. bracteolata flower extract can produce valuable AgNPs. The characteristic features of these AgNPs were assessed with UV-visible spectrophotometer, Fourier transform-infrared spectroscopy, Transmission Electron Microscope, Scanning Electron Microscopy, as well as. Under UV-vis. spectrum results, showed major peak at 430 nm and recorded essential functional groups responsible for reducing, capping, and stabilizing AgNPs by FT-IR analysis...
March 14, 2024: Environmental Research
https://read.qxmd.com/read/38492643/factors-associated-with-the-prevalence-of-salmonella-generic-escherichia-coli-and-coliforms-in-florida-s-agricultural-soils
#3
JOURNAL ARTICLE
Clara M Diekman, Camryn Cook, Laura K Strawn, Michelle D Danyluk
Limited data exist on the environmental factors that impact pathogen prevalence in the soil. The prevalence of foodborne pathogens, Salmonella and Listeria monocytogenes, and the prevalence and concentration of generic E. coli in Florida's agricultural soils was evaluated to understand the potential risk of microbial contamination at the pre-harvest level. For all organisms but L. monocytogenes, a longitudinal field study was performed in three geographically distributed agricultural areas across Florida. At each location, 20 unique 5x5 m field sampling sites were selected, soil was collected and evaluated for Salmonella presence (25 g) and E...
March 14, 2024: Journal of Food Protection
https://read.qxmd.com/read/38492480/to-what-extent-do-water-reuse-treatments-reduce-antibiotic-resistance-indicators-a-comparison-of-two-full-scale-systems
#4
JOURNAL ARTICLE
Ishi Keenum, Jeanette Calarco, Haniyyah Majeed, E Eldridge Hager-Soto, Charles Bott, Emily Garner, Valerie J Harwood, Amy Pruden
Water reuse is an essential strategy for reducing water demand from conventional sources, alleviating water stress, and promoting sustainability, but understanding the effectiveness of associated treatment processes as barriers to the spread of antibiotic resistance is an important consideration to protecting human health. We comprehensively evaluated the reduction of antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria (ARB) in two field-operational water reuse systems with distinct treatment trains, one producing water for indirect potable reuse (ozone/biologically-active carbon/granular activated carbon) and the other for non-potable reuse (denitrification-filtration/chlorination) using metagenomic sequencing and culture...
March 5, 2024: Water Research
https://read.qxmd.com/read/38491992/high-carriage-of-plasmid-mediated-quinolone-resistance-pmqr-genes-by-esbl-producing-and-fluoroquinolone-resistant-escherichia-coli-recovered-from-animal-waste-dumps
#5
JOURNAL ARTICLE
Elizabeth Omokoshi Joel, Olabisi Comfort Akinlabi, Adedolapo Victoria Olaposi, Temitayo Omotunde Olowomofe, Abimbola Olumide Adekanmbi
BACKGROUND: There has been a rise in the consumption of fluoroquinolones in human and veterinary medicine recently. This has contributed to the rising incidence of quinolone resistance in bacteria. This study aimed at the determination of the antibiotic resistance profile of ESBL-producing and fluoroquinolone-resistant E. coli (FQEC) isolated from animal waste obtained from the waste dumps of an agricultural farm and their carriage of genes encoding PMQR. METHODS AND RESULTS: Isolation of ESBL-producing E...
March 16, 2024: Molecular Biology Reports
https://read.qxmd.com/read/38491556/derivation-of-a-novel-antimicrobial-peptide-from-the-red-sea-brine-pools-modified-to-enhance-its-anticancer-activity-against-u2os-cells
#6
JOURNAL ARTICLE
Mona Elradi, Ahmed I Ahmed, Ahmed M Saleh, Khaled M A Abdel-Raouf, Lina Berika, Yara Daoud, Asma Amleh
Cancer associated drug resistance is a major cause for cancer aggravation, particularly as conventional therapies have presented limited efficiency, low specificity, resulting in long term deleterious side effects. Peptide based drugs have emerged as potential alternative cancer treatment tools due to their selectivity, ease of design and synthesis, safety profile, and low cost of manufacturing. In this study, we utilized the Red Sea metagenomics database, generated during AUC/KAUST Red Sea microbiome project, to derive a viable anticancer peptide (ACP)...
March 15, 2024: BMC Biotechnology
https://read.qxmd.com/read/38491194/phyto-fabricated-zno-nanoparticles-for-anticancer-photo-antimicrobial-effect-on-carbapenem-resistant-sensitive-pseudomonas-aeruginosa-and-removal-of-tetracycline
#7
JOURNAL ARTICLE
Gopinath Venkatraman, Priyadarshini Sakthi Mohan, Maryam Mohammed Mashghan, Kar-Cheng Wong, Puteri Shafinaz Abdul-Rahman, Kumutha Malar Vellasamy, Abdurahman Hajinur Hirad, Abdullah A Alarfaj, Shifa Wang
Alternanthera sessilis (AS) leaf extract was used to synthesize zinc oxide nanoparticles (ZnO NPs). Bioanalytical characterization techniques such as X-ray diffraction (XRD) and field emission scanning electron microscope (FESEM) confirmed the formation of crystalline ZnO NPs with average sizes of 40 nm. The AS-ZnO NPs antimicrobial activity was analyzed under dark (D) and white light (WL) conditions. The improved antimicrobial activity was observed against Escherichia coli, Staphylococcus aureus and Bacillus subtilis at the minimal inhibitory concentration (MIC) of 125 and 62...
March 16, 2024: Bioprocess and Biosystems Engineering
https://read.qxmd.com/read/38491116/phytochemical-composition-and-in-vitro-antioxidant-and-antimicrobial-activities-of-bersama-abyssinica-f-seed-extracts
#8
JOURNAL ARTICLE
Belayhun Alemu, Meseret Derbew Molla, Hiwot Tezera, Aman Dekebo, Tadesse Asmamaw
Medicinal plants can be potential sources of therapeutic agents. Traditional healers use a medicinal plant from Ethiopia, Bersama abyssinica Fresen, to treat various diseases. This study aimed to investigate the phytochemical components and antioxidant and antimicrobial activities of B. abyssinica seed extracts (BASE). Gas chromatography coupled to mass spectroscopy (GC-MS) analysis was used to determine the phytochemical compositions of BASE. The antioxidant activities were assessed by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, thiobarbituric acid-reactive species (TBARS) assay, ferric chloride reducing assay and hydroxyl scavenging capacity assay...
March 15, 2024: Scientific Reports
https://read.qxmd.com/read/38491103/role-of-gut-derived-bacterial-lipopolysaccharide-and-peripheral-tlr4-in-immobilization-stress-induced-itch-aggravation-in-a-mouse-model-of-atopic-dermatitis
#9
JOURNAL ARTICLE
Da-Eun Cho, Joon-Pyo Hong, Yoongeun Kim, Ju Yeon Sim, Heenam Stanley Kim, Song-Rae Kim, Bombi Lee, Hyo-Sung Cho, Ik-Hyun Cho, Sooan Shin, Mijung Yeom, Soon-Kyeong Kwon, In-Seon Lee, Hijoon Park, Kyuseok Kim, Dae-Hyun Hahm
Psychological stress and intestinal leakage are key factors in atopic dermatitis (AD) recurrence and exacerbation. Here, we demonstrate the mechanism underlying bacterial translocation across intestinal epithelial barrier damaged due to stress and further aggravation of trimellitic anhydride (TMA)-induced itch, which remain unclear, in AD mice. Immobilization (IMO) stress exacerbated scratching bouts and colon histological damage, and increased serum corticosterone and lipopolysaccharide (LPS). Orally administered fluorescein isothiocyanate (FITC)-dextran and surgically injected (into the colon) Cy5...
March 15, 2024: Scientific Reports
https://read.qxmd.com/read/38491007/a-citric-acid-cycle-deficient-escherichia-coli-as-an-efficient-chassis-for-aerobic-fermentations
#10
JOURNAL ARTICLE
Hang Zhou, Yiwen Zhang, Christopher P Long, Xuesen Xia, Yanfen Xue, Yanhe Ma, Maciek R Antoniewicz, Yong Tao, Baixue Lin
Tricarboxylic acid cycle (TCA cycle) plays an important role for aerobic growth of heterotrophic bacteria. Theoretically, eliminating TCA cycle would decrease carbon dissipation and facilitate chemicals biosynthesis. Here, we construct an E. coli strain without a functional TCA cycle that can serve as a versatile chassis for chemicals biosynthesis. We first use adaptive laboratory evolution to recover aerobic growth in minimal medium of TCA cycle-deficient E. coli. Inactivation of succinate dehydrogenase is a key event in the evolutionary trajectory...
March 15, 2024: Nature Communications
https://read.qxmd.com/read/38490558/high-resolution-melting-real-time-pcr-assays-for-subtyping-of-five-diarrheagenic-escherichia-coli-by-a-single-well-in-milk
#11
JOURNAL ARTICLE
Shan Shan, Rui Li, Weicheng Xia, Xiaoyu Tong, Yanmei Huang, Yucheng Tan, Silu Peng, Chengwei Liu, Shuanglong Wang, Daofeng Liu
Diarrheagenic Escherichia coli (DEC) is a kind of foodborne pathogen that poses a significant threat to both food safety and human health. To address the current challenges of high prevalence and difficult subtyping of DEC, this study developed a method that combined multiplex polymerase chain reaction (PCR) with high resolution melting (HRM) analysis for subtyping 5 kinds of DEC. The target genes are amplified by multiplex PCR in a single well, and HRM curve analysis was applied for distinct amplicons based on different melting temperature (Tm) values...
March 13, 2024: Journal of Dairy Science
https://read.qxmd.com/read/38490435/differences-between-bacteria-and-eukaryotes-in-clamp-loader-mechanism-a-conserved-process-underlying-dna-replication
#12
JOURNAL ARTICLE
Jacob T Landeck, Joshua Pajak, Emily K Norman, Emma L Sedivy, Brian A Kelch
Clamp loaders are pentameric ATPases that place circular sliding clamps onto DNA, where they function in DNA replication and genome integrity. The central activity of a clamp loader is the opening of the ring-shaped sliding clamp, and the subsequent binding to primer-template (p/t)-junctions. The general architecture of clamp loaders is conserved across all life, suggesting that their mechanism is retained. Recent structural studies of the eukaryotic clamp loader Replication Factor C (RFC) revealed that it functions using a crab-claw mechanism, where clamp opening is coupled to a massive conformational change in the loader...
March 13, 2024: Journal of Biological Chemistry
https://read.qxmd.com/read/38490287/study-on-the-therapeutic-mechanism-of-hj-granules-in-a-rat-model-of-urinary-tract-infection-caused-by-escherichia-coli
#13
JOURNAL ARTICLE
Shan Cao, Shuangrong Gao, Chen Ni, Yingli Xu, Bo Pang, Jingsheng Zhang, Yu Zhang, Yaxin Wang, Zihan Geng, Shurang Li, Ronghua Zhao, Bing Han, Xiaolan Cui, Yanyan Bao
ETHNOPHARMACOLOGICAL RELEVANCE: Urinary tract infections (UTIs) are globally prevalent infectious diseases, predominantly caused by uropathogenic Escherichia coli (UPEC). The misuse of antibiotics has led to the emergence of several drug-resistant strains. Traditional Chinese Medicine (TCM) has its own advantages in the treatment of UTIs. HJ granules is a herbal formula used for the treatment of UTIs. However, its mechanism of action is not clear. AIM OF THE STUDY: The aim of this study was to investigate the therapeutic efficacy and mechanism of action of HJ granules in a rat model of UTI caused by Escherichia coli (E coli) CFT073...
March 13, 2024: Journal of Ethnopharmacology
https://read.qxmd.com/read/38489878/carbon-dots-and-covalent-organic-frameworks-based-fret-immunosensor-for-sensitive-detection-of-escherichia-coli-o157-h7
#14
JOURNAL ARTICLE
Sunli Wang, Nini Liang, Xuetao Hu, Wenting Li, Ziang Guo, Xinai Zhang, Xiaowei Huang, Zhihua Li, Xiaobo Zou, Jiyong Shi
The combination of carbon dots (CDs) with covalent organic frameworks (COFs) was used to design an innovative sensor based on fluorescence resonance energy transfer (FRET) for the detection of Escherichia coli O157:H7 (E. coli O157:H7) in food samples. Carbon dots were used as fluorescence donors, covalent organic frameworks as fluorescence acceptors. The antibody (Ab) specific to E. coli O157:H7 was used to form a CD-Ab-COF immunosensor by linking CDs and COFs. The antibody was specifically bound with E. coli O157:H7, which caused the connection between CDs and COFs to be interrupted, and the carbon dots exhibited fluorescence restoration...
February 7, 2024: Food Chemistry
https://read.qxmd.com/read/38489813/the-identification-of-risk-factors-and-outcomes-of-cerebrospinal-fluid-shunt-infections-caused-by-carbapenem-resistant-gram-negative-bacteria-in-children-a-retrospective-cohort
#15
JOURNAL ARTICLE
Gizem Guner Ozenen, Zumrut Sahbudak Bal, Elif Bolat, Zuhal Umit, Nimet M Bilen, Sema Yildirim Arslan, Tuncer Turhan, Feriha Cilli, Zafer Kurugol
OBJECTIVE: Cerebrospinal fluid (CSF) shunt infections caused by gram-negative bacteria are difficult to treat given the limited treatment options and the emergence of carbapenem-resistant (CR) strains. This study aimed to evaluate the demographic and clinical characteristics of children with CSF shunt and external ventricular drain (EVD) infections caused by gram-negative bacteria, to identify the risk factors for acquiring CR CSF shunt infections, and to report on the clinical outcomes of these infections...
March 15, 2024: Journal of Neurosurgery. Pediatrics
https://read.qxmd.com/read/38489099/biofilm-producing-and-carbapenems-resistant-escherichia-coli-nosocomial-uropathogens-a-cross-sectional-study
#16
JOURNAL ARTICLE
Doaa Abo-Alella, Wessam Abdelmoniem, Enas Tantawy, Ahmed Asaad
OBJECTIVES: This cross-sectional study aims to determine the incidence and potential risk factors associated with biofilm-producing uropathogenic Escherichia coli (UPEC) nosocomial strains from a tertiary care hospital and to examine the prospective correlation between biofilm generation and antibiotic resistance phenotypes and genotypes. METHODS: A total of 130 UPEC nosocomial isolates were identified, their biofilm formation was quantified using a modified microtiter plate assay, and their antibiotic susceptibilities were assessed utilizing the disc diffusion method...
March 15, 2024: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://read.qxmd.com/read/38488502/transient-binding-dynamics-of-complement-system-pattern-recognition-molecules-on-pathogens
#17
JOURNAL ARTICLE
Maximilian Peter Götz, Mario Alejandro Duque Villegas, Beatrice Fageräng, Aileen Kerfin, Mikkel-Ole Skjoedt, Peter Garred, Anne Rosbjerg
Previous studies of pattern recognition molecules (PRMs) of the complement system have revealed difficulties in observing binding on pathogens such as Aspergillus fumigatus and Escherichia coli, despite complement deposition indicative of classical and lectin pathway activation. Thus, we investigated the binding dynamics of PRMs of the complement system, specifically C1q of the classical pathway and mannose-binding lectin (MBL) of the lectin pathway. We observed consistently increasing deposition of essential complement components such as C4b, C3b, and the terminal complement complex on A...
March 15, 2024: Journal of Immunology
https://read.qxmd.com/read/38488375/spectrum-and-antibiotic-resistance-in-community-and-hospital-acquired-urinary-tract-infections-among-adults-experience-from-a-large-tertiary-care-center-in-a-developing-country
#18
JOURNAL ARTICLE
Aya Herbawi, Adham Abu Taha, Banan M Aiesh, Ali Sabateen, Sa'ed H Zyoud
BACKGROUND: It is important to note that the causative agents and patterns of antibiotic resistance vary between urinary tract infections (UTIs) acquired in the community and those acquired in a hospital setting. Therefore, the aim of this study was to compare the types of organisms and patterns of antibiotic resistance in adult patients with community-acquired urinary tract infections (CA-UTIs) and hospital-acquired urinary tract infections (HA-UTIs). METHODS: Retrospectively, we collected urine samples from patients at An-Najah National University Hospital who experienced nonrecurring urinary tract infections (UTIs) between January 2019 and December 2020...
March 15, 2024: Urologia
https://read.qxmd.com/read/38488359/comparison-of-metagenomic-and-traditional-methods-for-diagnosis-of-e-coli-enteric-infections
#19
JOURNAL ARTICLE
C Royer, N V Patin, K J Jesser, A Peña-Gonzalez, J K Hatt, G Trueba, K Levy, K T Konstantinidis
UNLABELLED: Diarrheagenic Escherichia coli , collectively known as DEC, is a leading cause of diarrhea, particularly in children in low- and middle-income countries. Diagnosing infections caused by different DEC pathotypes traditionally relies on the cultivation and identification of virulence genes, a resource-intensive and error-prone process. Here, we compared culture-based DEC identification with shotgun metagenomic sequencing of whole stool using 35 randomly drawn samples from a cohort of diarrhea-afflicted patients...
March 15, 2024: MBio
https://read.qxmd.com/read/38488061/a-capillary-based-centrifugal-indicator-equipped-with-in-situ-pathogenic-bacteria-culture-for-fast-antimicrobial-susceptibility-testing
#20
JOURNAL ARTICLE
Longyu Chen, Meijia Zhu, Zhiyong Wang, Hongliang Wang, Yongqiang Cheng, Ziwei Zhang, Xiaoxiao Qi, Yifan Shao, Xi Zhang, Hongwei Wang
Antimicrobial resistance has become a major global health threat due to the misuse and overuse of antibiotics. Rapid, affordable, and high-efficiency antimicrobial susceptibility testing (AST) is among the effective means to solve this problem. Herein, we developed a capillary-based centrifugal indicator (CBCI) equipped with an in situ culture of pathogenic bacteria for fast AST. The bacterial incubation and growth were performed by macro-incubation, which seamlessly integrated the capillary indicator. Through simple centrifugation, all the bacterial cells were confined at the nanoliter-level capillary column...
March 15, 2024: Analyst
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