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https://www.readbyqxmd.com/read/28732043/investigation-of-horizontal-gene-transfer-of-pathogenicity-islands-in-escherichia-coli-using-next-generation-sequencing
#1
Maxim Messerer, Wolfgang Fischer, Sören Schubert
Horizontal gene transfer (HGT) contributes to the evolution of bacteria. All extraintestinal pathogenic Escherichia coli (ExPEC) harbour pathogenicity islands (PAIs), however relatively little is known about the acquisition of these PAIs. Due to these islands, ExPEC have properties to colonize and invade its hosts efficiently. Even though these PAIs are known to be acquired by HGT, only very few PAIs do carry mobilization and transfer genes required for the transmission by HGT. In this study, we apply for the first time next-generation sequencing (NGS) and in silico analyses in combination with in vitro experiments to decipher the mechanisms of PAI acquisition in ExPEC...
2017: PloS One
https://www.readbyqxmd.com/read/28731845/rho-protein-roles-and-mechanisms
#2
Pallabi Mitra, Gairika Ghosh, Md Hafeezunnisa, Ranjan Sen
At the end of the multistep transcription process, the elongating RNA polymerase (RNAP) is dislodged from the DNA template either at specific DNA sequences, called the terminators, or by a nascent RNA-dependent helicase, Rho. In Escherichia coli, about half of the transcription events are terminated by the Rho protein. Rho utilizes its RNA-dependent ATPase activities to translocate along the mRNA and eventually dislodges the RNAP via an unknown mechanism. The transcription elongation factor NusG facilitates this termination process by directly interacting with Rho...
July 21, 2017: Annual Review of Microbiology
https://www.readbyqxmd.com/read/28731528/improving-succinate-productivity-by-engineering-a-cyanobacterial-co2-concentrating-system-ccm-in-escherichia-coli
#3
Mengyong Xiao, Xinna Zhu, Changhao Bi, Yanhe Ma, Xueli Zhang
Biologically fixation of CO2 has great potential as a significant carbon source for biosynthesis, which is also a major way to reduce CO2 accumulation in atmosphere. Phosphoenolpyruvate (PEP) carboxylation is the key step of anaerobic succinate production in Escherichia coli. In this reaction, one mole CO2 is assimilated with PEP to form oxaloacetate by PEP carboxykinase (PCK). The preferred substrate of PCK is CO2 , which is very limited in cytoplasm. In this study, the carbon concentration mechanism (CCM) of cyanobacteria was introduced into E...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28731324/using-infrared-spectroscopy-and-multivariate-analysis-to-detect-antibiotics-resistant-escherichia-coli-bacteria
#4
Uraib Sharaha, Eladio Rodriguez-Diaz, Klaris Riesenberg, Irving J Bigio, Mahmoud Huleihel, Ahmad Salman
Bacterial pathogens are one of the primary causes of human morbidity worldwide. Historically, antibiotics have been highly effective against most bacterial pathogens; however, the increasing resistance of bacteria to a broad spectrum of commonly used antibiotics has become a global health-care problem. Early and rapid determination of bacterial susceptibility to antibiotics has become essential in many clinical settings, and, sometimes, can save lives. Currently classical procedures require at least 48 hours for determining bacterial susceptibility, which can constitute a life-threatening delay for effective treatment...
July 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28730473/expression-of-recombinant-phosphoproteins-for-signal-transduction-studies
#5
Karl W Barber, Jesse Rinehart
Complex signaling cascades are difficult to study in vitro without phosphorylated proteins. Here, we describe a technique for the routine production of recombinant phosphoproteins by directly incorporating phosphoserine as a nonstandard amino acid. This protocol utilizes an optimized phosphoserine orthogonal translation system and an engineered strain of E. coli containing no genomic amber codons. This approach has been used to generate a variety of phosphorylated proteins to understand the role of protein phosphorylation in cell signaling...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28730370/the-n-terminal-and-central-domain-of-colicin-a-enables-phage-lysin-to-lyse-escherichia-coli-extracellularly
#6
Guangmou Yan, Jianfang Liu, Qiang Ma, Rining Zhu, Zhimin Guo, Chencheng Gao, Shuang Wang, Ling Yu, Jingmin Gu, Dongliang Hu, Wenyu Han, Rui Du, Junling Yang, Liancheng Lei
Multidrug-resistant Escherichia coli has seriously threatened antibiotic resources and international public health. Bacteriophage lysin preparations have been widely considered as valid agents for solving multidrug resistances. Many lysins have been derived to treat diseases caused by Gram-positive bacteria, but only a few lysin preparations have been found that successively treat diseases caused by Gram-negative bacteria. The outer membrane of Gram-negative bacteria effectively blocks the interactions between peptidoglycan in the periplasmic space and bacteriophage lysins, which therefore hampers the antimicrobial effects of bacteriophage lysins...
July 20, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28730135/clinical-characteristics-and-ultrasonographic-findings-of-acute-bacterial-enterocolitis-in-children
#7
Peter Chun, Taek Jin Lim, Eun Ha Hwang, Sang Wook Mun, Yeoun Joo Lee, Jae Hong Park
PURPOSE: This study clarified the bacterial pathogens currently causing acute infectious enterocolitis (AIE) in children and evaluated the clinical characteristics and ultrasonographic findings according to the different pathogens. METHODS: Medical records regarding age, sex, clinical symptoms, laboratory data, identified enteropathogens, ultrasonographic findings, treatment, and outcome of 34 patients who were diagnosed with AIE via stool examination using multiplex polymerase chain reaction (PCR) or culture, were retrospectively reviewed...
June 2017: Pediatric Gastroenterology, Hepatology & Nutrition
https://www.readbyqxmd.com/read/28729761/designing-of-a-penta-peptide-against-drug-resistant-e-coli
#8
Sachin Nagra, Deepak Kumar, Rajasri Bhattacharyya, Dibyajyoti Banerjee, Tapan Mukherjee
Drug resistant pathogens are vibrant global problem. Penicillin binding protein 5 (PBP5) plays important role in bacterial cell wall biosynthesis. Mutation in PBP5 is a well-known mechanism for development of drug resistant strain of bacteria. In this context we have designed a peptide that fits better at the ligand-binding site of mutant PBP5 compared to wild type PBP5. It is expected that the designed peptide will halt the growth of drug resistant pathogen harboring mutant variety of PBP5. We have recommended experimental validation of the above concept...
2017: Bioinformation
https://www.readbyqxmd.com/read/28729676/proteomic-profiling-of-the-weed-feverfew-a-neglected-pollen-allergen-source
#9
Isabel Pablos, Stephanie Eichhorn, Peter Briza, Claudia Asam, Ulrike Gartner, Martin Wolf, Christof Ebner, Barbara Bohle, Naveen Arora, Stefan Vieths, Fatima Ferreira, Gabriele Gadermaier
Feverfew (Parthenium hysterophorus), an invasive weed from the Asteraceae family, has been reported as allergen source. Despite its relevance, knowledge of allergens is restricted to a partial sequence of a hydroxyproline-rich glycoprotein. We aimed to obtain the entire sequence for recombinant production and characterize feverfew pollen using proteomics and immunological assays. Par h 1, a defensin-proline fusion allergen was obtained by cDNA cloning and recombinantly produced in E. coli. Using two complementary proteomic strategies, a total of 258 proteins were identified in feverfew pollen among those 47 proteins belonging to allergenic families...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28729216/self-assembled-aunps-for-ingrain-pigmentation-of-silk-fabrics-with-antibacterial-potency
#10
Hanan B Ahmed, Nancy S El-Hawary, Hossam E Emam
Nanogold was self-assembled for ingrain pigmentation of silk fabric with antibacterial properties. The functional groups of silk macromolecular polymeric chains were exploited for generation and stabilization of nanogold. Hydrogen peroxide was used for activation of silk molecular chains through increasing their reactivity and accessibility to be functionalized as manufacturer of nanogold without using any external agents. Effect of procedure sequencing was studied through addition of peroxide firstly (group A) and with Au salt (group B)...
July 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28729208/detection-of-multidrug-resistant-mdr-bacteria-in-untreated-waste-water-disposals-of-hospitals-in-dhaka-city-bangladesh
#11
M A G Rabbani, Md Zakir Hossain Hawlader, Yearul Kabir
BACKGROUND: Emergence of MDR bacteria is a global problem and a major burden for treatment of various infectious diseases. This study was performed to detect antibiotic resistant bacteria in untreated hospital waste. METHOD: Waste water samples were collected from sewerage disposal points of two renowned hospital of Dhaka city and a total of 59 Escherichia coli and 29 Klebsiella pneumonia isolates were isolated. Antibiotic susceptibility were measured by disc diffusion method...
July 17, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28729206/antimicrobial-resistance-patterns-of-gram-negative-bacteria-isolated-from-bloodstream-infections-in-an-iranian-referral-pediatric-hospital-a-six-year-study
#12
Shima Mahmoudi, Masoumeh Mahzari, Maryam Banar, Babak Pourakbari, Mohammad Taghi Haghi Ashtiani, Mohsen Mohammadi, Sepideh Keshavarz Valian, Setareh Mamishi
INTRODUCTION: Bloodstream infections (BSIs) are considered as a major cause of morbidity and mortality in children all over the world. The aim of this study was to describe the epidemiology and antimicrobial resistance patterns of Gram-negative bacteria (GNB) isolated from bloodstream infections (BSIs) in children admitted to the Children Medical Center Hospital (CMC), Tehran, Iran. METHODS: Clinical and microbiological data of the patients with positive blood cultures was collected from March 2011 to September 2016...
July 17, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28728566/genomic-fingerprints-of-escherichia-coli-strains-isolated-from-surface-water-in-alborz-province-iran
#13
Reza Ranjbar, Parichehr Pezeshknejad, Faham Khamesipour, Kiumars Amini, Roohollah Kheiri
BACKGROUND: Consistent use of suitable diagnostic methods is essential to evaluate the genomic diversity of E. coli strains. Advance of efficient methods to discriminate the causes of E. coli in aquatic environments is important. This study aimed to describe the strain diversity of an E. coli population retrieved from surface water. METHODS: One hundred water samples were drawn within a period of 1 year, from May 2012 to May 2013, and E. coli bacteria have been isolated from water samples...
July 20, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28728309/escherichia-coli-outer-membrane-protein-f-ompf-an-immunogenic-protein-induces-cross-reactive-antibodies-against-escherichia-coli-and-shigella
#14
Xiao Wang, Da Teng, Qingfeng Guan, Ruoyu Mao, Ya Hao, Xiumin Wang, Junhu Yao, Jianhua Wang
Diarrhea caused by pathogenic Escherichia coli (E. coli) is one of the most serious infectious diseases in humans and animals. Due to antibiotics resistance and the lack of efficient vaccine, more attention should be paid to find potential versatile vaccine candidates to prevent diseases. In this study, the sequence homology analysis indicated that OmpF from E. coli CVCC 1515 shares a high identity (90-100%) with about half of the E. coli (46.7%) and Shigella (52.8%) strains. Then the recombinant OmpF was supposed to be developed as a versatile vaccine to prevent E...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28728277/-effects-of-pi3k%C3%AE-rhoa-pathway-on-phagocytosis-defect-of-alveolar-macrophages-in-a-mouse-model-of-chronic-obstructive-pulmonary-disease
#15
S T Hu, Q Xia, X L Zeng, H R Bao, X J Liu
Objective: To investigate effects of Phosphoinositide3-Kinases (PI3Kδ)-Ras homolog gene family member A(RhoA) pathway on phagocytosis deficiency of alveolar macrophages (AMs) in a mouse model of chronic obstructive pulmonary disease (COPD). Methods: Twenty mice were exposed to cigarette smoking to establish the COPD model, with 20 mice as the control group. AMs were isolated from lung tissue by discontinuous density gradient centrifugation and then divided into a healthy control group, a COPD group, a healthy IC87114 group and a COPD IC87114 group...
July 12, 2017: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28728058/high-prevalence-of-esbls-in-retail-chicken-meat-despite-reduced-use-of-antimicrobials-in-chicken-production-france
#16
Tiago Casella, Mara Correa Lelles Nogueira, Estelle Saras, Marisa Haenni, Jean-Yves Madec
Extended-spectrum cephalosporins (ESCs) are critically important antibiotics for humans and their use in animals poses a potential threat for public health. Chicken represents an increasing part of the human diet and has also been regarded as a source of ESC-resistant Enterobacteriaceae because of the worldwide off-label use of ceftiofur, a broad-spectrum cephalosporin. Thus, numerous studies pointed out chicken as a reservoir of ESBL/pAmpC genes, plasmids and/or clones at risk for humans. In France, late 2011, strong political pressure led to a drastic reduction of ceftiofur use and all other antibiotics in chicken production...
July 10, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28728044/atomic-force-microscopy-study-of-morphological-modifications-induced-by-different-decontamination-treatments-on-escherichia-coli
#17
Florina Lucica Zorila, Cristina Ionescu, Liviu Stefan Craciun, Bogdan Zorila
In this paper we used atomic force microscopy (AFM) to investigate the surface morphology of Escherichia coli, after being subjected to decontamination treatments, at sub-MICs levels (minimal inhibitory concentrations), with different disinfectants used in hospitals, pharmaceutical, food industry and even in our home, as an essential means to prevent the spreading of microorganisms. This article focuses on different morphological modifications adopted by E. coli cells as responses to the different modes of action of these substances...
July 14, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/28728009/bridged-rebar-graphene-functionalized-aptasensor-for-pathogenic-e-coli-o78-k80-h11-detection
#18
Harmanjit Kaur, Munish Shorie, Manju Sharma, Ashok K Ganguli, Priyanka Sabherwal
We report a novel fabrication method of functionalised Bridged Rebar Graphene (BRG) onto newly designed nanostructured aptasensor for label free impedimetric sensing of pathogenic bacteria E. coli O78:K80:H11. The chemical facilitated unscrolling of MWCNT and subsequent bridging with terephthalaldehyde (TPA) to form 3D-hierarchical BRG nanoconstruct exhibited synergistic effect by combining enhanced electrical properties and facile chemical functionality for stable bio-interface. The bacteria-DNA interactions were captured on BRG nanostructured electrode by using specific anti-E...
July 5, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28727911/ultrasound-processing-of-liquid-system-s-and-its-antimicrobial-mechanism-of-action
#19
David Spiteri, Claire Chot-Plassot, Julie Sclear, Kimon Andreas Karatzas, Chris Scerri, Vasilis Valdramidis
Ultrasound creates cavitation phenomena, resulting in the formation of several free radicals, namely OH• and H•, due to the breakdown of the H2 O molecule. These radicals affect the cellular integrity of the bacteria, causing the inactivation of several processes, and thus it is important to unravel the mechanism of action of this technology. This research looks into the application and mechanism of action of ultrasound technology as a means of disinfection by acoustic cavitation. Sterile water and synthetic waste water were inoculated with different mutants of E...
July 20, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28727781/escherichia-coli-mastitis-strains-in-vitro-phenotypes-and-severity-of-infection-in-vivo
#20
Perrine Roussel, Adeline Porcherie, Maryline Répérant-Ferter, Patricia Cunha, Christophe Gitton, Pascal Rainard, Pierre Germon
Mastitis remains a major infection of dairy cows and an important issue for dairy farmers and the dairy industry, in particular infections due to Escherichia coli strains. So far, properties specific to E. coli causing mastitis remain ill defined. In an attempt to better understand the properties required for E. coli to trigger mastitis, we used a range of in vitro assays to phenotypically characterize four E. coli strains, including the prototypical E. coli mastitis strain P4, possessing different relative abilities to cause mastitis in a mouse model...
2017: PloS One
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