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https://www.readbyqxmd.com/read/28231686/evaluation-of-the-amoxicillin-concentrations-in-amniotic-fluid-placenta-umbilical-cord-blood-and-maternal-serum-two-hours-after-intravenous-administration
#1
(no author information available yet)
OBJECTIVES: The aims of this study were to evaluate amoxicillin concentrations in amniotic fluid, placenta, umbilical cord blood and maternal blood two hours after intravenous administration to assess obstetric and non-obstetric factors that could have influences on the penetration of the antibiotic into the examined tissues and to analyze the sensitivity to amoxicillin of the most common pathogens isolated from the genital tract. METHODS: A total of 35 full-term pregnant women who qualified for elective Caesarean delivery were included in the study...
October 24, 2016: Neuro Endocrinology Letters
https://www.readbyqxmd.com/read/28231311/handwashing-and-ebola-virus-disease-outbreaks-a-randomized-comparison-of-soap-hand-sanitizer-and-0-05-chlorine-solutions-on-the-inactivation-and-removal-of-model-organisms-phi6-and-e-coli-from-hands-and-persistence-in-rinse-water
#2
Marlene K Wolfe, Karin Gallandat, Kyle Daniels, Anne Marie Desmarais, Pamela Scheinman, Daniele Lantagne
To prevent Ebola transmission, frequent handwashing is recommended in Ebola Treatment Units and communities. However, little is known about which handwashing protocol is most efficacious. We evaluated six handwashing protocols (soap and water, alcohol-based hand sanitizer (ABHS), and 0.05% sodium dichloroisocyanurate, high-test hypochlorite, and stabilized and non-stabilized sodium hypochlorite solutions) for 1) efficacy of handwashing on the removal and inactivation of non-pathogenic model organisms and, 2) persistence of organisms in rinse water...
2017: PloS One
https://www.readbyqxmd.com/read/28231167/antibacterial-activity-of-fructus-forsythia-essential-oil-and-the-application-of-eo-loaded-nanoparticles-to-food-borne-pathogens
#3
Na Guo, Qing-Yan Gai, Jiao Jiao, Wei Wang, Yuan-Gang Zu, Yu-Jie Fu
Fructus forsythia essential oil (FEO) with excellent antibacterial activity was rarely reported. The objective of the present study was to investigate the antibacterial activity and the antibacterial mechanism of FEO against two food-borne pathogenic bacteria, Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) in vitro. When treated FEO, the zones of inhibition (ZOI) of E. coli (20.5 ± 0.25 mm) and S. aureus (24.3 ± 0.21 mm) were much larger than control (p < 0.05). The minimum inhibitory concentrations (MICs) of FEO were 3...
October 29, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28231137/evaluation-of-electrolytically-generated-hypochlorous-acid-electrolyzed-water-for-sanitation-of-meat-and-meat-contact-surfaces
#4
Shawnna Veasey, Peter M Muriana
'Electrolyzed water' generators are readily available in the food industry as a renewable source of hypochlorous acid that eliminates the need for workers to handle hazardous hypochlorite concentrates. We applied electrolyzed water (EW) directly to multi-strain cocktails of Listeria monocytogenes, E. coli O157:H7, and Salmonella sp. at 250 ppm free available chlorine (FAC) and achieved greater than 6-log reductions in 2 min. Lower EW values were examined as antimicrobial interventions for fresh meat (beef carcasses), processed meats (frankfurters), and food contact surfaces (slicing blades)...
June 13, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28230975/tuning-of-recombinant-protein-expression-in-escherichia-coli-by-manipulating-transcription-translation-initiation-rates-and-incorporation-of-non-canonical-amino-acids
#5
Orr Schlesinger, Yonatan Chemla, Mathias Heltberg, Eden Ozer, Ryan Marshall, Vincent Noireaux, Mogens Hogh Jensen, Lital Alfonta
Protein synthesis in cells has been thoroughly investigated and characterized over the past 60 years. However, some fundamental issues remain unresolved, including the reasons for genetic code redundancy and codon bias. In this study, we changed the kinetics of the E. coli transcription and translation processes by mutating the promoter and ribosome binding domains and by using genetic code expansion. The results expose a counterintuitive phenomenon, whereby an increase in the initiation rates of transcription and translation lead to a decrease in protein expression...
February 23, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28230769/hypoallergenic-variant-of-the-major-egg-white-allergen-gal-d-1-produced-by-disruption-of-cysteine-bridges
#6
Pathum Dhanapala, Dulashi Withanage-Dona, Mimi L K Tang, Tim Doran, Cenk Suphioglu
Gal d 1 (ovomucoid) is the dominant allergen in the chicken egg white. Hypoallergenic variants of this allergen can be used in immunotherapy as an egg allergy treatment approach. We hypothesised that disruption of two of the nine cysteine-cysteine bridges by site-directed mutagenesis will allow the production of a hypoallergenic variant of the protein; Methods: Two cysteine residues at C192 and C210 in domain III of the protein were mutated to alanine using site-directed mutagenesis, to disrupt two separate cysteine-cysteine bridges...
February 21, 2017: Nutrients
https://www.readbyqxmd.com/read/28230642/strategies-to-obtain-diverse-and-specific-human-monoclonal-antibodies-from-transgenic-animals
#7
Marianne Brüggemann, Michael J Osborn, Biao Ma, Roland Buelow
Techniques to obtain large quantities of antigen-specific monoclonal antibodies, mAbs, were first established in the 1970s when Georges Köhler and César Milstein immortalized antibody-producing mouse B-lymphocytes by fusion with myeloma cells (http://www.whatisbiotechnology.org/exhibitions/milstein). Combined with the expression of human antibodies in transgenic animals, this technique allowed upon immunization the generation of highly specific fully human mAbs for therapeutic applications. Apart from being extremely beneficial, mAbs are a huge success commercially...
February 23, 2017: Transplantation
https://www.readbyqxmd.com/read/28230345/antibacterial-performance-of-a-pcl-pdmaema-blend-nanofiber-based-scaffold-enhanced-with-immobilized-silver-nanoparticles
#8
Fernanda Grandzolli Santos, Leticia Caroline Bonkovoski, Francielle P Garcia, Thelma Sley Pacheco Cellet, Maria Alice Witt, Celso Vataru Nakamura, Adley Forti Rubira, Edvani Curti Muniz
In the present study, nanofiber meshes (NFs), composed of polycaprolactone and poly[(2-dimethylamino) ethyl methacrylate] at 80/20 and 50/50 PCL/PDMAEMA blend ratios, were obtained through electrospinning. Silver nanoparticles (AgNPs) formed in situ were then immobilized on NF surfaces through adsorption processes, at different pHs. It was possible to observe that the amount of NF-AgNPs can be tuned by changing the pH of AgNPs-immobilization and the PCL/PDMAEMA ratio in the blend. The neat NF and NF-AgNPs were characterized with respect to their morphology and mechanical properties...
February 23, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28230084/highly-potent-antimicrobial-peptides-from-n-terminal-membrane-binding-region-of-e-coli-mreb
#9
Karabi Saikia, Yalavarthi Durga Sravani, Vibin Ramakrishnan, Nitin Chaudhary
Microbial pathogenesis is a serious health concern. The threat escalates as the existing conventional antimicrobials are losing their efficacy against the evolving pathogens. Peptides hold promise to be developed into next-generation antibiotics. Antimicrobial peptides adopt amphipathic structures that could selectively bind to and disrupt the microbial membranes. Interaction of proteins with membranes is central to all living systems and we reasoned that the membrane-binding domains in microbial proteins could be developed into efficient antimicrobials...
February 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28229589/production-of-cinnamyl-alcohol-glucoside-from-glucose-in-escherichia-coli
#10
Wei Zhou, Huiping Bi, Yibin Zhuang, Qinglin He, Hua Yin, Tao Liu, Yanhe Ma
Rosin, a cinnamyl alcohol glucoside, is one of the important ingredients in Rhodiola rosea, which is a valuable medicinal herb used for centuries. Rosin displayed multiple biological activities. The traditional method for producing rosin and derivatives is direct extraction from R. rosea, which suffers from limited availability of natural resources and complicated purification procedure. In this work, we achieved de novo biosynthesis of rosin in Escherichia coli. Firstly, a biosynthetic pathway of aglycon cinnamyl alcohol from phenylalanine was constructed...
February 23, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28229045/occurrence-of-giardia-cryptosporidium-and-entamoeba-in-wild-rhesus%C3%A2-macaques-macaca-mulatta-living-in-urban-and-semi-rural-north-west-india
#11
John J Debenham, Kristoffer Tysnes, Sandhya Khunger, Lucy J Robertson
Giardia duodenalis, Cryptosporidium spp., and Entamoeba spp. are intestinal protozoa capable of infecting a range of host species, and are important causes of human morbidity and mortality. Understanding their epidemiology is important, both for public health and for the health of the animals they infect. This study investigated the occurrence of these protozoans in rhesus macaques (Macaca mulatta) in India, with the aim of providing preliminary information on the potential for transmission of these pathogens between macaques and humans...
April 2017: International Journal for Parasitology. Parasites and Wildlife
https://www.readbyqxmd.com/read/28228648/spondyloarthropathies-e-coli-links-ibd-to-spondyloarthritis
#12
Dario Ummarino
No abstract text is available yet for this article.
February 23, 2017: Nature Reviews. Rheumatology
https://www.readbyqxmd.com/read/28228322/anti-tumour-research-of-recombinant-bcg-using-bzlf1-and-hgm-csf-fusion-genes
#13
Qing-Jie Xue, Yun-Qing Li, Chun-Qing Yang, Ting Chen, Xiu-Zhen Li, Baohua Cheng, Chun-Mei Wang
The random primer Oligo(dT)15 was used in RT-PCR to obtain cDNA from the human granulocyte macrophage colony stimulating factor (hGM-CSF) gene and the Epstein-Barr virus (EBV) gene BZLF1. Then, the sequence splicing overlap extension method was used to obtain a GCBF fusion gene containing a linker sequence that encoded the polypeptide (Gly4Ser)3. The GCBF fusion gene was inserted into pMV261, which was then transformed into competent E. coli DH5 alpha cells, and positive cells were selected based on kanamycin resistance on LB plates...
February 19, 2017: Vaccine
https://www.readbyqxmd.com/read/28228181/characterization-and-functional-analysis-of-serpin-1-like-gene-from-oak-silkworm-antheraea-pernyi
#14
H M Yu, B J Zhu, Y Sun, G Q Wei, L Wang, C Qian, M Nadeem Abbas, C L Liu
Serpins are a broadly distributed family of proteases found in various organisms that play an important role in regulating the immune response. Here, we identified a serpin-1 gene from Antheraea pernyi that encodes a 279 amino acid protein with a molecular weight of 30.8 kDa. We expressed the recombinant Ap-serpin-1 protein in Escherichia coli and used the purified protein to prepare rabbit anti-Ap-serpin-1 polyclonal antibodies. We calculated the enzyme-linked immunosorbent assay titer of the antibody as 1:128000...
February 23, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28227908/a-boolean-approach-to-bacterial-chemotaxis
#15
Anuj Deshpande, Sibendu Samanta, Haimabati Das, Ritwik Kumar Layek, Anuj Deshpande, Sibendu Samanta, Haimabati Das, Ritwik Kumar Layek, Sibendu Samanta, Anuj Deshpande, Haimabati Das, Ritwik Kumar Layek
Bacterium such as Escherichia coli (E. coli) show biased Brownian motion in different chemical concentration gradients. This chemical sensitive motility or chemotaxis has gained considerable interest among scientists for some remarkable features such as chemo-sensory dynamic range, adaptation, diffusion and drift. A Boolean model of the whole chemotaxis process has been developed in this manuscript. The response of the circuit is in accordance with the experimental results available in the literature, providing indirect validation of the model...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226208/tailoring-escherichia-coli-for-the-l-rhamnose-pbad-promoter-based-production-of-membrane-and-secretory-proteins
#16
Anna Hjelm, Alexandros Karyolaimos, Zhe Zhang, Edurne Rujas, David Vikström, Dirk Jan Slotboom, Jan-Willem de Gier
Membrane and secretory protein production in Escherichia coli requires precisely controlled production rates to avoid the deleterious saturation of their biogenesis pathways. Based on this requirement, the E. coli L-rhamnose PBAD promoter (PrhaBAD) is often used for membrane and secretory protein production since PrhaBAD is thought to regulate protein production rates in an L-rhamnose concentration dependent manner. By monitoring protein production in real-time in E. coli wild-type and an L-rhamnose catabolism deficient mutant, we demonstrate that the L-rhamnose concentration-dependent tunability of PrhaBAD-mediated protein production is actually due to L-rhamnose consumption rather than regulating production rates...
February 22, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28226206/exploration-of-the-innate-immune-system-of-styela-clava-zn-2-binding-enhances-the-antimicrobial-activity-of-the-tunicate-peptide-clavanin-a
#17
Samuel A Juliano, Scott Pierce, James A DeMayo, Marcy J Balunas, Alfredo M Angeles-Boza
Tunicates have been used as primitive models for understanding cell mediated and humoral immunity. Clavanin A (ClavA) is a potent antimicrobial peptide produced by the solitary tunicate Styela clava. In this work, we demonstrate that ClavA utilizes Zn(2+) ions to potentiate its antimicrobial activity. A reduced concentration at which the peptide inhibits the growth of bacteria and an increase in the rate of killing is observed in the presence of Zn(2+). Membrane depolarization, β-galactosidase leakage and potassium leakage assays indicate that ClavA is membrane active, forms small pores, but induces cell death by targeting an intracellular component...
February 22, 2017: Biochemistry
https://www.readbyqxmd.com/read/28225615/structural-characterization-and-in-vitro-fermentation-of-%C3%AE-mannooligosaccharides-produced-from-locust-bean-gum-by-gh-26-endo-%C3%AE-1-4-mannanase-manb-1601
#18
Praveen Kumar Srivastava, Deepesh Panwar, Harish Prashanth Kv, Mukesh Kapoor
Size exclusion chromatography of β-mannooligosaccharides (β-MOS) mixture, obtained from ManB-1601 hydrolysis of locust bean gum, resulted in separation of oligosaccharides with varied degree of polymerization (DP 2, 3 and 5). The oligosaccharides were structurally [ESI-MS, FTIR, XRD, TGA and NMR (1H and 13C)] and functionally (in vitro fermentation) characterized. DP2 oligosaccharide was composed of two species (A) mannopyranose β-1,4 mannopyranose and (B) α-1,6-galactosyl-mannopyranose, while DP3 oligosaccharide showed presence of only one species i...
February 22, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28225595/colorimetric-and-electrochemical-bacteria-detection-using-printed-paper-and-transparency-based-analytic-devices
#19
Jaclyn A Adkins, Katherine Boehle, Colin Friend, Briana Chamberlain, Bledar Bisha, Charles S Henry
The development of transparency-based electrochemical and paper-based colorimetric analytic detection platforms is presented as complimentary methods for food and waterborne bacteria detection from a single assay. Escherichia coli and Enterococcus species, both indicators of fecal contamination, were detected using substrates specific to enzymes produced by each species. β-galactosidase (β-gal) and β-glucosidase (β-gluco) are both produced by E. coli, while β-glucosidase (β-gluco) is produced by Enterococcus spp...
February 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28225132/an-unexpected-use-of-ferrocene-a-scanning-electrochemical-microscopy-study-of-a-toll-like-receptor-array-and-its-interaction-with-e-coli
#20
Zhe She, Kristin Topping, Bin Dong, Mohtashim H Shamsi, Heinz-Bernhard Kraatz
Imaging of toll-like receptor microarrays was achieved using scanning electrochemical microscopy with the successful integration of two ferrocene derivatives in order to enhance the background contrast. This investigation has resulted in the novel fabrication of a tuneable, multiplex, broad-spectrum bacterial sensor for the interrogation of conserved microbial stimuli.
February 22, 2017: Chemical Communications: Chem Comm
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