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https://www.readbyqxmd.com/read/29141238/rationale-redesign-of-type-iii-secretion-systems-toward-the-development-of-non-pathogenic-e-coli-for-in-vivo-delivery-of-therapeutic-payloads
#1
REVIEW
Coral González-Prieto, Cammie F Lesser
Transkingdom secretion systems that bacteria use to inject proteins directly into the cytosol of mammalian host cells play an essential role in the virulence of many Gram-negative bacterial pathogens. Current efforts are underway to repurpose these machines as novel therapeutics; type III secretion systems as vectors for the delivery of proteins of therapeutic value including heterologous antigens for vaccine development and type IV secretion systems as vectors for DNA. While initial studies focused on the use of attenuated or replication incompetent pathogens, the recent development of non-pathogenic Escherichia coli that encode programmable type III secretion systems expands possibilities for the in vivo directed delivery of therapeutic payloads...
November 12, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/29141107/metal-transformation-as-a-strategy-for-bacterial-detoxification-of-heavy-metals
#2
Ashraf M M Essa, Mohamed A Al Abboud, Sayeed I Khatib
Microorganisms can modify the chemical and physical characters of metals leading to an alteration in their speciation, mobility, and toxicity. Aqueous heavy metals solutions (Hg, Cd, Pb, Ag, Cu, and Zn) were treated with the volatile metabolic products (VMPs) of Escherichia coli Z3 for 24 h using aerobic bioreactor. The effect of the metals treated with VMPs in comparison to the untreated metals on the growth of E. coli S1 and Staphylococcus aureus S2 (local isolates) was examined. Moreover, the toxic properties of the treated and untreated metals were monitored using minimum inhibitory concentration assay...
November 15, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/29141051/molecular-and-epidemiological-characterization-of-carbapenemase-producing-enterobacteriaceae-in-norway-2007-to-2014
#3
Ørjan Samuelsen, Søren Overballe-Petersen, Jørgen Vildershøj Bjørnholt, Sylvain Brisse, Michel Doumith, Neil Woodford, Katie L Hopkins, Bettina Aasnæs, Bjørg Haldorsen, Arnfinn Sundsfjord
The prevalence of carbapenemase-producing Enterobacteriaceae (CPE) is increasing worldwide. Here we present associated patient data and molecular, epidemiological and phenotypic characteristics of all CPE isolates in Norway from 2007 to 2014 confirmed at the Norwegian National Advisory Unit on Detection of Antimicrobial Resistance. All confirmed CPE isolates were characterized pheno- and genotypically, including by whole genome sequencing (WGS). Patient data were reviewed retrospectively. In total 59 CPE isolates were identified from 53 patients...
2017: PloS One
https://www.readbyqxmd.com/read/29140975/natural-variation-of-a-sensor-kinase-controlling-a-conserved-stress-response-pathway-in-escherichia-coli
#4
Manuela Roggiani, Srujana S Yadavalli, Mark Goulian
Previous studies have shown that exponentially growing Escherichia coli can detect mild acidity (~pH 5.5) and, in response, synthesize enzymes that protect against severe acid shock. This adaptation is controlled by the EvgS/EvgA phosphorelay, a signal transduction system present in virtually every E. coli isolate whose genome has been sequenced. Here we show that, despite this high level of conservation, the EvgS/EvgA system displays a surprising natural variation in pH-sensing capacity, with some strains entirely non-responsive to low pH stimulus...
November 15, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/29140786/selection-of-an-anticalin%C3%A2-against-the-membrane-form-of-hsp70-via-bacterial-surface-display-and-its-theranostic-application-in-tumour-models
#5
Lars Friedrich, Petra Kornberger, Claudia T Mendler, Gabriele Multhoff, Markus Schwaiger, Arne Skerra
We describe the selection of Anticalins against a common tumour surface antigen, human Hsp70, using functional display on live E. coli cells as fusion with a truncated EspP autotransporter. While found intracellularly in normal cells, Hsp70 is frequently exposed in a membrane-bound state on the surface of tumour cells and, even more pronounced, in metastases or after radiochemotherapy. Employing a recombinant Hsp70 fragment comprising residues 383-548 as target, Anticalins were selected from a naive bacterial library...
November 27, 2017: Biological Chemistry
https://www.readbyqxmd.com/read/29140746/inactivation-kinetics-of-pathogens-during-thermal-processing-in-acidified-broth-and-tomato-pur%C3%A3-e-ph-4-5
#6
Evann L Dufort, Jonathan Sogin, Mark R Etzel, Barbara H Ingham
Thermal inactivation kinetics for single strains of Shiga toxin-producing Escherichia coli (STEC), Listeria monocytogenes, and Salmonella enterica were measured in acidified tryptic soy broth (TSB; pH 4.5) heated at 54°C. Inactivation curves also were measured for single-pathogen five-strain cocktails of E. coli O157:H7, L. monocytogenes, and S. enterica heated in tomato purée (pH 4.5) at 52, 54, 56, and 58°C. Inactivation curves were fit using log-linear and nonlinear (Weibull) models. The Weibull model yields the time for a 5-log reduction (t*) and a curve shape parameter (β)...
November 15, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/29140539/cross-regulation-among-arabinose-xylose-and-rhamnose-utilization-systems-in-e-coli
#7
Debika Choudhury, Supreet Saini
Bacteria frequently encounter multiple sugars in their natural surroundings. While the dynamics of utilization of glucose containing sugar mixtures have been well investigated, there are few reports addressing regulation of utilization of glucose-free mixtures particularly pentoses. These sugars comprise a considerable fraction in hemicellulose which can be converted by suitable biocatalysts to biofuels and other value-added products. Hence understanding of transcriptional cross-regulation among different pentose sugar utilization systems is essential for successful development of industrial strains...
November 15, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29140461/a-bacterial-three-hybrid-assay-detects-escherichia-coli-hfq-srna-interactions-in-vivo
#8
Katherine E Berry, Ann Hochschild
The interaction of RNA molecules with proteins is a critical aspect of gene regulation across all domains of life. Here, we report the development of a bacterial three-hybrid (B3H) assay to genetically detect RNA-protein interactions. The basis for this three-hybrid assay is a transcription-based bacterial two-hybrid assay that has been used widely to detect and dissect protein-protein interactions. In the three-hybrid assay, a DNA-bound protein with a fused RNA-binding moiety (the coat protein of bacteriophage MS2 (MS2CP)) is used to recruit a hybrid RNA upstream of a test promoter...
November 11, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29140285/chitosan-cyclodextrin-tpp-nanoparticles-loaded-with-quercetin-as-novel-bacterial-quorum-sensing-inhibitors
#9
Hao Thanh Nguyen, Francisco M Goycoolea
The widespread emergence of antibiotic-resistant bacteria has highlighted the urgent need of alternative therapeutic approaches for human and animal health. Targeting virulence factors that are controlled by bacterial quorum sensing (QS), seems a promising approach. The aims of this study were to generate novel nanoparticles (NPs) composed of chitosan (CS), sulfo-butyl-ether-β-cyclodextrin (Captisol(®)) and/or pentasodium tripolyphosphate using ionotropic gelation technique, and to evaluate their potential capacity to arrest QS in bacteria...
November 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29139529/lab-on-capillary-a-rapid-simple-and-quantitative-genetic-analysis-platform-integrating-nucleic-acid-extraction-amplification-and-detection
#10
Yu Fu, Xiaoming Zhou, Da Xing
In this work, we describe for the first time a genetic diagnosis platform employing a polydiallyldimethylammonium chloride (PDDA)-modified capillary and a liquid-based thermalization system for rapid, simple and quantitative DNA analysis with minimal user interaction. Positively charged PDDA is modified on the inner surface of the silicon dioxide capillary by using an electrostatic self-assembly approach that allows the negatively charged DNA to be separated from the lysate in less than 20 seconds. The capillary loaded with the PCR mix is incorporated in the thermalization system, which can achieve on-site real-time PCR...
November 15, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/29139345/herbicide-ingredients-change-salmonella-enterica-sv-typhimurium-and-escherichia-coli-antibiotic-responses
#11
Brigitta Kurenbach, Paddy S Gibson, Amy M Hill, Adam S Bitzer, Mark W Silby, William Godsoe, Jack A Heinemann
Herbicides are frequently released into both rural and urban environments. Commercial herbicide formulations induce adaptive changes in the way bacteria respond to antibiotics. Salmonella enterica sv. Typhimurium and Escherichia coli were exposed to common co-formulants of formulations, and S. enterica sv. Typhimurium was exposed to active ingredients dicamba, 2,4-D and glyphosate to determine what ingredients of the commercial formulations caused this effect. Co-formulants Tween80 and carboxymethyl cellulose induced changes in response, but the pattern of the responses differed from the active ingredients, and effect sizes were smaller...
November 17, 2017: Microbiology
https://www.readbyqxmd.com/read/29139344/aeropyrum-pernix-membrane-topology-of-protein-vkor-promotes-protein-disulfide-bond-formation-in-two-subcellular-compartments
#12
Stijntje Hibender, Cristina Landeta, Mehmet Berkmen, Jon Beckwith, Dana Boyd
Disulfide bonds confer stability and activity to proteins. Bioinformatic approaches allow predictions of which organisms make protein disulfide bonds and in which subcellular compartments disulfide bond formation takes place. Such an analysis, along with biochemical and protein structural data, suggests that many of the extremophile Crenarachaea make protein disulfide bonds in both the cytoplasm and the cell envelope. We have sought to determine the oxidative folding pathways in the sequenced genomes of the Crenarchaea, by seeking homologues of the enzymes known to be involved in disulfide bond formation in bacteria...
November 15, 2017: Microbiology
https://www.readbyqxmd.com/read/29139018/vaccination-against-hydatidosis-molecular-cloning-and-optimal-expression-of-the-eg95nc-recombinant-antigen-in-escherichia-coli
#13
M Jazouli, M Lightowlers, C G Gauci, K Tadlaoui, A Belmlih, M M Ennaji, M Elharrak
Cystic echinococcosis (CE) is a widely distributed zoonosis that is highly endemic in the Mediterranean basin. The disease represents a serious public health threat and causes economic losses. The parasite life-cycle involves dogs and ruminants as definitive and intermediate hosts; humans are accidently infected, causing serious clinical issues. Vaccination of ruminants and dog treatments represent the most efficient measures to prevent parasite transmission. The recombinant protein vaccine, EG95, has been used successfully in sheep vaccine trials against CE in several countries...
November 14, 2017: Protein Journal
https://www.readbyqxmd.com/read/29138983/cloning-expression-analysis-and-enzyme-activity-assays-of-the-%C3%AE-carbonic-anhydrase-gene-from-chlamydomonas-sp-ice-l
#14
Changfeng Qu, Yingying He, Zhou Zheng, Meiling An, Lulu Li, Xixi Wang, Xiaodong He, Yibin Wang, Fangming Liu, Jinlai Miao
The α-carbonic anhydrase (α-CA) is a zinc ion-containing enzyme that catalyzes the hydration of carbon dioxide. In this paper, a full-length α-CA gene was cloned from Chlamydomonas sp. ICE-L using RT-PCR and RACE-PCR for bioinformatic analysis. The α-CA open reading frame obtained by PCR was cloned into a vector and transformed into Escherichia coli to generate α-CA-producing bacteria. The α-CA was highly expressed upon induction with isopropyl-β-d-thiogalactoside (IPTG) at a final concentration of 0...
November 14, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/29138692/transcriptomics-driven-lipidomics-tdl-identifies-the-microbiome-regulated-targets-of-ileal-lipid-metabolism
#15
Anirikh Chakrabarti, Mathieu Membrez, Delphine Morin-Rivron, Jay Siddharth, Chieh Jason Chou, Hugues Henry, Stephen Bruce, Sylviane Metairon, Frederic Raymond, Bertrand Betrisey, Carole Loyer, Scott J Parkinson, Mojgan Masoodi
The gut microbiome and lipid metabolism are both recognized as essential components in the maintenance of metabolic health. The mechanisms involved are multifactorial and (especially for microbiome) poorly defined. A strategic approach to investigate the complexity of the microbial influence on lipid metabolism would facilitate determination of relevant molecular mechanisms for microbiome-targeted therapeutics. E. coli is associated with obesity and metabolic syndrome and we used this association in conjunction with gnotobiotic models to investigate the impact of E...
2017: NPJ Systems Biology and Applications
https://www.readbyqxmd.com/read/29138582/once-daily-intramuscular-amikacin-for-outpatient-treatment-of-lower-urinary-tract-infections-caused-by-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-in-children
#16
Meltem Polat, Soner Sertan Kara
Background: The rise in community-acquired urinary tract infections (UTIs) with extended-spectrum β-lactamase (ESBL)-producing Escherichia coli strains raises the question of how to treat these infections effectively in pediatric outpatients. Amikacin has shown promising in vitro activity against ESBL-producing urinary isolates of E. coli; however, clinical data are limited. Objective: To investigate the clinical and microbiological outcomes of community-acquired lower UTIs caused by ESBL-producing E...
2017: Infection and Drug Resistance
https://www.readbyqxmd.com/read/29138562/dual-uv-irradiation-based-metal-oxide-nanoparticles-for-enhanced-antimicrobial-activity-in-escherichia-coli-and-m13-bacteriophage
#17
Su-Eon Jin, Woochul Hwang, Hyo Jung Lee, Hyo-Eon Jin
Metal oxide (MO) nanoparticles have been studied as nano-antibiotics due to their antimicrobial activities even in antibiotic-resistant microorganisms. We hypothesized that a hybrid system of dual UV irradiation and MO nanoparticles would have enhanced antimicrobial activities compared with UV or MO nanoparticles alone. In this study, nanoparticles of ZnO, ZnTiO3, MgO, and CuO were selected as model nanoparticles. A dual UV collimated beam device of UV-A and UV-C was developed depending upon the lamp divided by coating...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29138496/designing-ecofriendly-bionanocomposite-assembly-with-improved-antimicrobial-and-potent-on-site-zika-virus-vector-larvicidal-activities-with-its-mode-of-action
#18
Pramod C Mane, Ravindra D Chaudhari, Manish D Shinde, Deepali D Kadam, Chung Kil Song, Dinesh P Amalnerkar, Haiwon Lee
Dialyzed natural polymer, fibroin, from Bombyx mori was used to synthesize biocompatible silver and gold nanoparticles in-situ in dispersion form. The films of pure fibroin (PF), fibroin-silver nanocomposite (FSNC) and fibroin-gold nanocomposite (FGNC) were fabricated by drop casting method. The characterization of the resultant dispersion and films was performed by visual color change, UV-Vis spectroscopy and atomic force microscopy. The dispersions of PF, FSNC and FGNC were tested for antibacterial activity against E...
November 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29138066/modification-of-membrane-properties-and-fatty-acids-biosynthesis-related-genes-in-escherichia-coli-and-staphylococcus-aureus-implications-for-the-antibacterial-mechanism-of-naringenin
#19
Lang-Hong Wang, Xin-An Zeng, Man-Sheng Wang, Charles S Brennan, Deming Gong
In this work, modifications of cell membrane fluidity, fatty acid composition and fatty acid biosynthesis-associated genes of Escherichia coli ATCC 25922 (E. coli) and Staphylococcus aureus ATCC 6538 (S. aureus), during growth in the presence of naringenin (NAR), one of the natural antibacterial components in citrus plants, was investigated. Compared to Escherichia coli, the growth of Staphylococcus aureus was significantly inhibited by NAR in low concentrations. Combination of gas chromatography-mass spectrometry with fluorescence polarization analysis revealed that E...
November 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29137796/construction-of-proton-exchange-membranes-under-ultrasonic-irradiation-based-on-novel-fluorine-functionalizing-sulfonated-polybenzimidazole-cellulose-silica-bionanocomposite
#20
Sheida Esmaielzadeh, Hashem Ahmadizadegan
Novel sulfonated polybenzimidazole (s-PBI)/cellulose/silica bionanocomposite membranes were prepared from fluorine-containing s-PBI copolymer with a cellulose/silica precursor and a bonding agent. The introduction of the bonding agent results in the reinforcing interfacial interaction between s-PBI chains and the cellulose/silica nanoparticles. Commercially available silica nanoparticles were modified with biodegradable nanocellolose through ultrasonic irradiation technique. Transmission electron microscopy (TEM) analyses showed that the cellulose/silica composites were well dispersed in the s-PBI matrix on a nanometer scale...
March 2018: Ultrasonics Sonochemistry
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