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https://www.readbyqxmd.com/read/27921337/extended-spectrum-cephalosporin-resistance-among-clinical-isolates-of-enterobacteriaceae-in-west-norway-during-2006-2013-a-prospective-surveillance-study
#1
Haima Mylvaganam, Helge Kolstad, Rebecca Irene Breistein, Grete Lind, Dag Harald Skutlaberg
Routine surveillance of resistance to broad-spectrum cephalosporins in Enterobacteriaceae and phenotypic identification of underlying mechanisms using a simple strategy was commenced in 2006 at our laboratory, serving West Norway. This report focuses on the results until 2013. The classical plasmid-mediated extended spectrum beta-lactamase (ESBLA ) among clinically relevant Escherichia coli isolates showed an increase from 0.6% to 4.3% during the surveillance period, while prevalence for other mechanisms remained stable, below 0...
December 6, 2016: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/27921270/halogenated-indoles-eradicate-bacterial-persister-cells-and-biofilms
#2
Jin-Hyung Lee, Yong-Guy Kim, Giyeon Gwon, Thomas K Wood, Jintae Lee
The emergence of antibiotic resistance has necessitated new therapeutic approaches to combat recalcitrant bacterial infections. Persister cells, often found in biofilms, are metabolically dormant, and thus, are highly tolerant to all traditional antibiotics and represent a major drug resistance mechanism. In the present study, 36 diverse indole derivatives were investigated with the aim of identifying novel compounds that inhibit persisters and biofilm formation by Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus...
December 2016: AMB Express
https://www.readbyqxmd.com/read/27921105/ultrasmall-silver-nanoclusters-highly-efficient-antibacterial-activity-and-their-mechanisms
#3
Jian-Cheng Jin, Xiao-Juan Wu, Juan Xu, Bei-Bei Wang, Feng-Lei Jiang, Yi Liu
Silver materials have been widely used as antimicrobial agents. Notably, silver nanoparticles have emerged as a new generation of nanoproducts for biomedical and environmental applications in recent years. However, ultrasmall silver nanoclusters (NCs) (∼2 nm) have rarely been used to kill bacteria and their antibacterial mechanisms have not yet been fully elucidated. Herein, we studied the antibacterial activities of bifunctional fluorescent DHLA-AgNCs against three types of bacteria. The results showed that DHLA-AgNCs exhibited excellent antibacterial activities against Gram-negative E...
December 6, 2016: Biomaterials Science
https://www.readbyqxmd.com/read/27921008/age-related-differences-in-the-luminal-and-mucosa-associated-gut-microbiome-of-broiler-chickens-and-shifts-associated-with-campylobacter-jejuni-infection
#4
Wageha A Awad, Evelyne Mann, Monika Dzieciol, Claudia Hess, Stephan Schmitz-Esser, Martin Wagner, Michael Hess
Despite the importance of gut microbiota for broiler performance and health little is known about the composition of this ecosystem, its development and response towards bacterial infections. Therefore, the current study was conducted to address the composition and structure of the microbial community in broiler chickens in a longitudinal study from day 1 to day 28 of age in the gut content and on the mucosa. Additionally, the consequences of a Campylobacter (C.) jejuni infection on the microbial community were assessed...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27920887/preliminary-study-on-cost-effective-l-tryptophan-production-from-indole-and-l-serine-by-e-coli-cells
#5
Tahereh Sadeghiyan-Rizi, Jamshid Fooladi, Sima Sadrai
BACKGROUND: L-tryptophan is used widespread in the pharmaceutical industry. The majority of L-Trp production depends on microbial processes that produce L-tryptophan from indole and L-serine. These processes are very costly due to the costs of precursors, especially L-serine. Use of inexpensive substitutions as the L-serine source of L-tryptophan production enables us to reach a cost-effective process. In this paper, effect of Triton X-100 on L-Trp production and the ability to use Iranian cane molasses as inexpensive L-serine source was investigated...
October 2016: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/27920886/evaluation-of-recombinant-human-growth-hormone-secretion-in-e-coli-using-the-l-asparaginase-ii-signal-peptide
#6
Mozhdeh Zamani, Navid Nezafat, Younes Ghasemi
BACKGROUND: In the recent years, there has been an increasing interest in secretory production of recombinant proteins, due to its various advantages compared with intracellular expression. Signal peptides play a critical role in prosperous secretion of recombinant proteins. Accordingly, different signal peptides have been assessed for their ability to produce secretory proteins by trial-and-error experiments. The aim of this study was to evaluate the effect of L-asparaginase II signal peptide on the recombinant human Growth Hormone (hGH) protein secretion in the Escherichia coli (E...
October 2016: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/27920885/cloning-and-expression-of-soluble-recombinant-hiv-1-crf35-protease-hp-thioredoxin-fusion-protein
#7
Asaad Azarnezhad, Zohreh Sharifi, Rahmatollah Seyedabadi, Arshad Hosseini, Behrooz Johari, Mahsa Sobhani Fard
BACKGROUND: As a drug target and an antigenic agent, HIV-1 protease (HIV-1 PR) is at the center of attention for designing anti-AIDS inhibitors and diagnostic tests. In previous studies, the production of the recombinant protease has been faced with several difficulties; therefore, the aims of this study were the easy production, purification of the soluble form of protease in E. coli and investigation of its immunoreactivity. METHODS: Protease coding region was isolated from the serum of an infected individual, amplified by RT-PCR and cloned into PTZ57R using TA-cloning...
October 2016: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/27920826/expression-and-purification-of-truncated-diphtheria-toxin-dt386-in-escherichia-coli-an-attempt-for-production-of-a-new-vaccine-against-diphtheria
#8
Fatemeh Shafiee, Mohammad Rabbani, Mahdi Behdani, Ali Jahanian-Najafabadi
The aim of this study was to produce a recombinant protein consisting of the catalytic and translocation domains of diphtheria toxin for its later application as a vaccine candidate against Corynebacterium diphtheria. To achieve this goal, at first, the amino acid sequence of DT386 was used for prediction of T and B cell epitopes using on-line servers. The DT386 coding sequence was synthesized and subcloned into the NcoI and XhoI sites of pET28a plasmid and recombinant pET28a plasmid was used to transform Escherichia coli BL21 (DE3) host cells...
October 2016: Research in Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27920824/cloning-expression-and-molecular-analysis-of-iranian-brucella-melitensis-omp25-gene-for-designing-a-subunit-vaccine
#9
Soheil Yousefi, Mojtaba Tahmoorespur, Mohammad Hadi Sekhavati
Brucellosis is a well-known domestic animal infectious disease, which is caused by Brucella bacterium. The outer membrane protein 25 kDa (Omp25) gene plays an important role in simulating of TNF-α, IFN-α, macrophage, and cytokines cells. In the current study molecular cloning and expression analysis of Omp25 gene for designing a subunit vaccine against Brucella was investigated. Amplifying the full length of candidate gene was performed using specific primers. Sub-cloning of this gene conducted using pTZ57R/T vector in TOP10F strain of Escherichia coli(E...
October 2016: Research in Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27920818/exposition-of-hepatitis-b-surface-antigen-hbsag-on-the-surface-of-hek293t-cell-and-evaluation-of-its-expression
#10
Mina Mirian, Razieh Taghizadeh, Hossein Khanahmad, Mansour Salehi, Ali Jahanian-Najafabadi, Hojjat Sadeghi-Aliabadi, Shirin Kouhpayeh
Hepatitis B virus (HBV) is considered as a global health concern and hepatitis B surface antigen (HBsAg) is the most immunogenic protein of HBV. The purpose of this study was to evaluate the expression of HBsAg on the cell surface of human embryonic kidney cell line (HEK293T). After transformation of expression vector pcDNA/HBsAg to E.coli TOP10F', plasmid was extracted and digested with BglII. Afterwards, the linearized vector was transfected to cells and treated with hygromycin B for 5 weeks to expand the resulted clonies...
October 2016: Research in Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27920754/culture-based-screening-of-aerobic-microbiome-in-diabetic-foot-subjects-and-developing-non-healing-ulcers
#11
Saba Noor, Jamal Ahmad, Iqbal Parwez, Maaz Ozair
The study was carried on diabetic foot patients to deduce clinical attributes, the occurrence of the range of aerobic microbial flora and to assess their comparative in vitro susceptibility to the customarily used antimicrobials. We also studied the potential risk factors involved in the development of non-healing ulcers. A total of 87 organisms were isolated from 70 specimens, including Escherichia coli (19.5%) among the Gram-negative and Staphylococcus aureus (18.4%) among the Gram-positive as the predominant aerobes explored...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27920296/a-sulfur-oxygenase-from-the-haloalkaliphilic-bacterium-thioalkalivibrio-paradoxus-with-atypically-low-reductase-activity
#12
Patrick Rühl, Uwe Pöll, Johannes Braun, Andreas Klingl, Arnulf Kletzin
: Sequence comparisons showed that the sulfur oxygenase reductase (SOR) of the haloalkaliphilic bacterium Thioalkalivibrio paradoxus Arh 1 (TpSOR) is branching deeply within dendrograms of these proteins (29-34% identity). A synthetic gene encoding the TpSOR expressed in E. coli resulted in a protein of 14.7±0.9 nm in diameter and an apparent molecular mass of 556 kDa. Sulfite and thiosulfate were formed from elemental sulfur in a temperature range of 10-98°C (Topt≈80°C) and a pH range of 6-11...
December 5, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27920212/inhibition-of-antigen-specific-and-non-specific-stimulation-of-bovine-t-and-b-cells-by-lymphostatin-from-attaching-and-effacing-escherichia-coli
#13
Robin L Cassady-Cain, Elizabeth A Blackburn, Charlotte R Bell, Elizaveta Elshina, Jayne C Hope, Mark P Stevens
Enterohaemorrhagic E. coli (EHEC) and enteropathogenic E. coli (EPEC) are enteric bacterial pathogens of worldwide importance. Most EPEC and non-O157 EHEC strains express lymphostatin (also known as LifA), a chromosomally-encoded 365 kDa protein. We previously demonstrated that lymphostatin is a putative glycosyltransferase that is important in intestinal colonisation of cattle by EHEC serogroup O5, O111 and O26 strains. However, the nature and consequences of the interaction between lymphostatin and immune cells from the bovine host are ill-defined...
December 5, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27919895/plazomicin-activity-against-346-extended-spectrum-%C3%AE-lactamase-ampc-producing-escherichia-coli-urinary-isolates-related-to-aminoglycoside-modifying-enzymes-characterized
#14
Maria Del Carmen López-Diaz, Esther Culebras, Iciar Rodríguez-Avial, Esther Rios, José Manuel Viñuela-Prieto, Juan J Picazo, Carmen Rodríguez-Avial
The activity of plazomicin and clinically relevant aminoglycosides were tested against 346 ESBL/AmpC-producer Escherichia coli urinary isolates and the results were correlated with the presence of aminoglycoside-modifying enzymes (AMEs).Data showed that plazomicin was very active against all ESBL/AmpC-producing E. coli urinary isolates. Its activity was not related to the AME genes studied.
December 5, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27919890/prevalence-of-esbl-and-or-carbapenemase-producing-escherichia-coli-isolated-from-yellow-legged-gulls-from-barcelona-spain
#15
Andrea Vergara, Cristina Pitart, Tomás Montalvo, Ignasi Roca, Sara Sabaté, Juan Carlos Hurtado, Raquel Planell, Francesc Marco, Beatriz Ramírez, Víctor Peracho, Mercé de Simón, Jordi Vila
Seventy two (54.5%) out of 132 fecal samples were positive for either extended spectrum beta-lactamases (ESBL) (51.5%), carbapenemase (1.5%) or cephamycinase (1.5%) producing Escherichia coli from a group of yellow-legged gulls in Barcelona, Spain. The isolation of two carbapenemase-producing E. coli strains is a matter of concern.
December 5, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27919692/improved-production-of-2-fucosyllactose-in-engineered-escherichia-coli-by-expressing-putative-%C3%AE-1-2-fucosyltransferase-wcfb-from-bacteroides-fragilis
#16
Young-Wook Chin, Ji-Yeong Kim, Jae-Han Kim, Sang-Min Jung, Jin-Ho Seo
2'-Fucosyllactose (2-FL) is one of most abundant oligosaccharides in human milk, which is involved in many biological functions for infant health. Since 2-FL has a great potential in application to functional food materials and pharmaceuticals, several microbial systems for mass production of 2-FL have been developed in recent years. Microbial production of 2-FL was suggested to be influenced by a number of factors including fucosylation activity of α-1,2-fucosyltransferase. In the present study, the wcfB gene coding for α-1,2-fucosyltransferase from Bacteroides fragilis was screened from eleven candidates of putative α-1,2-fucosyltransferase...
December 2, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27919686/structural-and-functional-study-of-chuy-from-escherichia-coli-strain-cft073
#17
Hun Kim, Akhilesh Kumar Chaurasia, Truc Kim, Jongkeun Choi, Sung Chul Ha, Doyoun Kim, Kyeong Kyu Kim
The uropathogenic Escherichia coli strain CFT073 contains multiple iron and heme transport systems, which facilitate infection of the host urinary tract. To elucidate the molecular and cellular function of ChuY, a hypothetical gene in the heme degradation/utilization pathway, we solved the crystal structure of ChuY at 2.4 Å resolution. ChuY has high structural homology with human biliverdin and flavin reductase. We confirmed that ChuY has flavin mononucleotide (FMN) reductase activity, using NAD(P)H as a cofactor, and shows porphyrin ring binding affinity...
December 2, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27919669/manothermosonication-mts-treatment-of-apple-carrot-juice-blend-for-inactivation-of-escherichia-coli-0157-h7
#18
Ozan Kahraman, Hyoungill Lee, Wei Zhang, Hao Feng
This study was performed to evaluate the responses of Escherichia coli 0157:H7 inoculated in an apple-carrot blended juice to manothermosonication (MTS) treatments. The MTS treatments were conducted in a continuous-flow MTS system. The juice samples were exposed to ultrasound treatment at combinations of three temperatures (60, 50 and 40°C) and three pressure levels (100, 200, and 300kPa) for five residence times (15, 30, 45, 60, and 75s). The results showed that higher treatment temperature (i.e. 60°C) and hydrostatic pressure in the MTS system significantly enhanced the microbial reduction...
November 24, 2016: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/27918986/hybrid-chitosan-polyaniline-polypyrrole-biomaterial-for-enhanced-adsorption-and-antimicrobial-activity
#19
Rajeev Kumar, Mohammad Oves, Talla Ameelbi, Naief H Al-Makishah, M A Barakat
In this work, chitosan (CS) functionalized polyaniline-polypyrrole (Pani-Ppy) copolymer (CS/Pani-Ppy) was synthesized applying a facile one pot method for the enhanced adsorption of Zn(II) and antimicrobial activity for E. coli and E. agglomerans. The synthesized materials were characterized using scanning electron microscopy, energy dispersive X-ray spectroscopy, Fourier transform inferred spectroscopy and X-ray photoelectron spectroscopy. The adsorption of the Zn(II) on the synthesized materials was highly dependent on the pH of the solution, the initial metal ion concentration, and temperature...
November 24, 2016: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/27918204/removal-of-listeria-monocytogenes-dual-species-biofilms-using-combined-enzyme-benzalkonium-chloride-treatments
#20
Pedro Rodríguez-López, Alba Carballo-Justo, Lorraine A Draper, Marta L Cabo
The effects of pronase (PRN), cellulase (CEL) or DNaseI alone or combined with benzalkonium chloride (BAC) against Listeria monocytogenes-carrying biofilms were assayed. The best removal activity against L. monocytogenes-Escherichia coli biofilms was obtained using DNaseI followed by PRN and CEL. Subsequently, a modified logistic model was used to quantify the combined effects of PRN or DNaseI with BAC. A better BAC performance after PRN compared to DNaseI eradicating L. monocytogenes was observed. In E. coli the effects were the opposite...
December 5, 2016: Biofouling
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