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https://www.readbyqxmd.com/read/29334612/tempo-oxidized-bacterial-cellulose-pellicle-with-silver-nanoparticles-for-wound-dressing
#1
Chun-Nan Wu, Shih-Chang Fuh, Shin-Ping Lin, Yen-Yi Lin, Hung-Yueh Chen, Jui-Ming Liu, Kuan-Chen Cheng
Biocompatible bacterial cellulose pellicle (BCP) is a candidate for biomedical material such as wound dressing. However, due to lack of antibacterial activity, to grant BCP with the property is crucial for its biomedical application. In the present study, BCP was modified by 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation using TEMPO/NaClO/NaBr system at pH 10 to form TEMPO-oxidized BCP (TOBCP) with anionic C6 carboxylate groups. The TOBCP was subsequently ion-exchanged in AgNO3 solution and silver nanoparticles (AgNP) with diameter of ~16...
January 15, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29334190/the-effects-of-low-levels-of-trivalent-ions-on-a-standard-strain-of-escherichia-coli-atcc-11775-in-aqueous-solutions
#2
Can Deng, Xinpeng Li, Xinkai Xue, Richard M Pashley
Considering the ever-growing usage of trivalent salts in water treatment, for example, lanthanum salts in rare earth, AlCl3 and FeCl3 , the effects of different trivalent cations on the bacterium Escherichia coli (E. coli) ATCC 11775 strain have been studied in aqueous solutions. From colony incubation studies, the colony-forming unit (CFU) densities were found to decrease significantly in the presence of even low levels (10-5  mol/L) of lanthanum chloride. This level of reduction in CFU number is comparable to the results obtained using the known bacteriocidal cationic surfactant, C14 TAB...
January 15, 2018: MicrobiologyOpen
https://www.readbyqxmd.com/read/29332999/an-integrated-electrolysis-electrospray-ionization-antimicrobial-platform-using-engineered-water-nanostructures-ewns-for-food-safety-applications
#3
Nachiket Vaze, Yi Jiang, Lucas Mena, Yipei Zhang, Dhimiter Bello, Stephen S Leonard, Anna M Morris, Mary Eleftheriadou, Georgios Pyrgiotakis, Philip Demokritou
Engineered water nanostructures (EWNS) synthesized utilizing electrospray and ionization of water, have been, recently, shown to be an effective, green, antimicrobial platform for surface and air disinfection, where reactive oxygen species (ROS), generated and encapsulated within the particles during synthesis, were found to be the main inactivation mechanism. Herein, the antimicrobial potency of the EWNS was further enhanced by integrating electrolysis, electrospray and ionization of de-ionized water in the EWNS synthesis process...
March 2018: Food Control
https://www.readbyqxmd.com/read/29332998/high-pressure-epr-spectroscopy-studies-of-the-e-coli-lipopolysaccharide-transport-proteins-lpta-and-lptc
#4
Kathryn M Schultz, Candice S Klug
The use of pressure is an advantageous approach to the study of protein structure and dynamics because it can shift the equilibrium populations of protein conformations toward higher energy states that are not of sufficient population to be observable at atmospheric pressure. Recently, the Hubbell group at the University of California, Los Angeles, reintroduced the application of high pressure to the study of proteins by electron paramagnetic resonance (EPR) spectroscopy. This methodology is possible using X-band EPR spectroscopy due to advances in pressure intensifiers, sample cells, and resonators...
December 2017: Applied Magnetic Resonance
https://www.readbyqxmd.com/read/29332592/construction-of-an-m1gs-ribozyme-for-targeted-and-rapid-mrna-cleavage-application-on-the-ets-2-oncogene
#5
Chrisavgi Toumpeki, Dimitrios Anastasakis, Ioannis Panagoulias, Vassiliki Stamatopoulou, Tassos Georgakopoulos, Sofia Kallia-Raftopoulos, Athanasia Mouzaki, Denis Drainas
Background RNase P-mediated cleavage of target RNAs has been proposed as a promising tool for gene silencing. Ets-2 proto-oncogene controls the expression of a wide variety of genes involved in cancer and immunity. Objective Construction of a functional RNase P-based ribozyme (M1GS303) that targets Ets-2 mRNA Method The accessible sites for targeting of Ets-2 mRNA were identified by footprinting analysis. M1GS303 ribozyme was constructed by cloning. The activity of the ribozyme in the presence or absence of spiramysin in E...
January 11, 2018: Medicinal Chemistry
https://www.readbyqxmd.com/read/29331911/enhanced-photocatalytic-degradation-of-sulfamethoxazole-by-zinc-oxide-photocatalyst-in-the-presence-of-fluoride-ions-optimization-of-parameters-and-toxicological-evaluation
#6
Amir Mirzaei, Laleh Yerushalmi, Zhi Chen, Fariborz Haghighat, Jianbo Guo
The presence of antibiotics in water bodies has received increasing attention since they are continuously introduced and detected in the environment and may cause unpredictable environmental hazards and risks. The photocatalytic degradation of sulfamethoxazole (SMX) by ZnO in the presence of fluoride ions (F-ZnO) was evaluated. The effects of operating parameters on the efficiency of SMX removal were investigated by using response surface methodology (RSM). Under the optimum condition, i.e. photocatalyst dosage = 1...
January 11, 2018: Water Research
https://www.readbyqxmd.com/read/29331783/comparative-study-of-novel-in-situ-decorated-porous-chitosan-selenium-scaffolds-and-porous-chitosan-silver-scaffolds-towards-antimicrobial-wound-dressing-application
#7
Dhee P Biswas, Neil M O'Brien-Simpson, Eric C Reynolds, Andrea J O'Connor, Phong A Tran
Dermal defects caused by trauma or disease are challenging to treat due to difficult-to-treat infections that impair wound healing. Due to the widespread emergence of drug-resistant bacteria and dwindling discoveries of new antibiotics, there is currently an urgent need to introduce novel antimicrobials effective against antibiotic-resistant bacteria without causing damage to host tissues. As selenium (Se) and silver (Ag) are known for their antimicrobial properties, we investigated the separate loading of these materials into porous chitosan/PVA (CS) scaffolds through a simple in situ deposition method to create two distinct wound dressing materials (CS-Se and CS-Ag)...
January 5, 2018: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29331487/curecuma-cationic-curcuminoids-with-improved-properties-and-enhanced-antimicrobial-photodynamic-activity
#8
Andreas Spaeth, Andre Graeler, Tim Maisch, Kristjan Plaetzer
The naturally occurring photosensitizer curcumin has excellent biocompatibility, but its antimicrobial photodynamic efficacy is limited by (i) weak adherence to Gram(-) cell walls, (ii) low (photo-)stability and (iii) limited solubility in water. In this study novel curcuminoids bearing cationic substituents were prepared by different synthetic routes. The derivatives exhibit excellent water solubility, improved photostability and low aggregation. All novel curcuminoids showed antibacterial photodynamic effects (>3 log10 reduction of CFU) against Escherichia coli and Staphylococcus aureus upon blue light illumination...
October 21, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29331374/alternative-pathway-of-h2s-and-polysulfides-production-from-sulfurated-catalytic-cysteine-of-reaction-intermediates-of-3-mercaptopyruvate-sulfurtransferase
#9
Noriyuki Nagahara, Shin Koike, Takashi Nirasawa, Hideo Kimura, Yuki Ogasawara
It has been known that hydrogen sulfide and/or polysulfides are produced from a (poly)sulfurated sulfur-acceptor substrate of 3-mercaptopyruvate sulfurtransferase (MST) via thioredoxin (Trx) reduction in vitro. In this study, we used thiosulfate as the donor substrate and the catalytic reaction was terminated on the formation of a persulfide or polysulfides. We can present alternative pathway of production of hydrogen sulfide and/or polysulfides from (poly)sulfurated catalytic-site cysteine of reaction intermediates of MST via Trx reduction...
January 10, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29331350/construction-of-pogoduet-an-inducible-bicistronic-vector-for-synthesis-of-recombinant-proteins-in-corynebacterium-glutamicum
#10
Oliver Goldbeck, Gerd M Seibold
The Gram-positive Corynebacterium glutamicum is widely known for its application in the industrial production of amino acids and as a non-pathogenic model organism for cell wall biosynthesis in the group of CMN bacteria. For biotechnological and physiological studies often co-expression of recombinant genes is required, however for C. glutamicum no vector for the independent co-expression of two genes was described. We here created the novel expression vector pOGOduet for C. glutamicum, which carries the ColE1 replicon of E...
January 10, 2018: Plasmid
https://www.readbyqxmd.com/read/29331014/biochemical-characterization-of-recombinant-thermostable-cohnella-sp-a01-%C3%AE-glucanase
#11
Meysam Rezaie, Saeed Aminzadeh, Farid Heidari, Masoud Mashhadi Akbar Boojar, Ali Asghar Karkhane
Background: Typically, non-cellulytic glucanase, including fungi and yeast cell wall hydrolyzing enzymes, are release by some symbiotic fungi and plants during the mycoparasitic fungi attack on plants. These enzymes are known as the defense mechanisms of plants. This study intends to investigate the biochemical properties of β-1,6-glucanase (bg16M) from native thermophilic bacteria, Cohnella A01. Methods: bg16M gene was cloned and expressed in E. coli BL21 (DE3)...
January 13, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29330529/the-interaction-network-of-the-yidc-insertase-with-the-secyeg-translocon-srp-and-the-srp-receptor-ftsy
#12
Narcis-Adrian Petriman, Benjamin Jauß, Antonia Hufnagel, Lisa Franz, Ilie Sachelaru, Friedel Drepper, Bettina Warscheid, Hans-Georg Koch
YidC/Oxa1/Alb3 are essential proteins that operate independently or cooperatively with the Sec machinery during membrane protein insertion in bacteria, archaea and eukaryotic organelles. Although the interaction between the bacterial SecYEG translocon and YidC has been observed in multiple studies, it is still unknown which domains of YidC are in contact with the SecYEG translocon. By in vivo and in vitro site-directed and para-formaldehyde cross-linking we identified the auxiliary transmembrane domain 1 of E...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29330519/large-scale-aggregation-analysis-of-eukaryotic-proteins-reveals-an-involvement-of-intrinsically-disordered-regions-in-protein-folding
#13
Eri Uemura, Tatsuya Niwa, Shintaro Minami, Kazuhiro Takemoto, Satoshi Fukuchi, Kodai Machida, Hiroaki Imataka, Takuya Ueda, Motonori Ota, Hideki Taguchi
A subset of the proteome is prone to aggregate formation, which is prevented by chaperones in the cell. To investigate whether the basic principle underlying the aggregation process is common in prokaryotes and eukaryotes, we conducted a large-scale aggregation analysis of ~500 cytosolic budding yeast proteins using a chaperone-free reconstituted translation system, and compared the obtained data with that of ~3,000 Escherichia coli proteins reported previously. Although the physicochemical properties affecting the aggregation propensity were generally similar in yeast and E...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29330189/targeted-synthesis-and-characterization-of-a-gene-cluster-encoding-nad-p-h-dependent-3%C3%AE-3%C3%AE-and-12%C3%AE-hydroxysteroid-dehydrogenases-from-eggerthella-cag-298-a-gut-metagenomic-sequence
#14
Sean M Mythen, Saravanan Devendran, Celia Méndez-García, Isaac Cann, Jason M Ridlon
Gut metagenomic sequences provide a rich source of microbial genes, the majority of which are annotated by homology or unknown. Genes and gene pathways that encode enzymes catalyzing biotransformation of host bile acids are important to identify in gut metagenomic sequences due to the importance of bile acids on gut microbiome structure and host physiology. Hydroxysteroid dehydrogenases (HSDH) are pyridine nucleotide-dependent enzymes with stereo-specificity and regio-specificity for bile acid and steroid hydroxyl groups...
January 12, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29330179/influence-of-type-i-fimbriae-and-fluid-shear-stress-on-bacterial-behavior-and-multicellular-architecture-of-early-escherichia-coli-biofilms-at-single-cell-resolution
#15
Liyun Wang, Robert Keatch, Qi Zhao, John A Wright, Clare E Bryant, Anna L Redmann, Eugene M Terentjev
Biofilm formation on abiotic surfaces in food and medical industry can cause severe contamination and infection, yet how biological and physical factors determine cellular architecture of early biofilms and bacterial behavior of the constituent cells remains largely unknown. In this study we examine the specific role of type-I fimbriae in nascent stages of biofilm formation and the response of micro-colonies to environmental flow shear at single-cell resolution. The results show that type-I fimbriae are not required for reversible adhesion from plankton, but critical for irreversible adhesion of Escherichia coli (E...
January 12, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29329972/enhanced-production-of-lactate-based-polyesters-in-escherichia-coli-from-a-mixture-of-glucose-and-xylose-by-mlc-mediated-catabolite-derepression
#16
Ryosuke Kadoya, Ken'ichiro Matsumoto, Kenji Takisawa, Toshihiko Ooi, Seiichi Taguchi
Lignocellulose-utilizing biorefinery is a promising strategy for the sustainable production of value-added products such as bio-based polymers. Simultaneous consumption of glucose and xylose in Escherichia coli was achieved by overexpression of the gene encoding Mlc, a multiple regulator of glucose and xylose uptake. This catabolite derepression gave the enhancement in the production of poly (15 mol% lactate-co-3-hydroxybutyrate), up to 65% from 50% (wild-type strain) in the cellular contents, of the Mlc-overexpressing strain of E...
January 9, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29329756/phospholipid-composition-of-the-outer-membrane-of-escherichia-coli-influences-its-susceptibility-against-antimicrobial-peptide-apidaecin-1b
#17
Rico Schmidt, Ding Yonghong, Ralf Hoffmann
Proline-rich antimicrobial peptides (PrAMPs) kill bacteria in a multimodal mechanism by inhibiting the 70S ribosome (i.e., protein translation) as dominant lethal mechanism besides inhibition of several other proteins, such as chaperone DnaK. PrAMPs pass the outer membrane of Gram-negative bacteria, probably by a self-promoted uptake followed by a transporter-mediated uptake from the periplasm. Mutation of transporter protein SbmA is a well-studied resistance mechanism observed in vitro by resistance induction with PrAMPs...
November 21, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29328997/synthesis-biological-evaluation-and-molecular-modeling-of-novel-thienopyrimidinone-and-triazolothienopyrimidinone-derivatives-as-dual-anti-inflammatory-antimicrobial-agents
#18
Adnan A Bekhit, Ahmed M Farghaly, Ragab M Shafik, Mona M A Elsemary, Alaa El-Din A Bekhit, Aida A Guemei, Mai S El-Shoukrofy, Tamer M Ibrahim
New thienopyrimidinone and triazolothienopyrimidinone derivatives have been synthesized. These compounds were subjected to anti-inflammatory and antimicrobial activity screening aiming to identify new candidates that have dual anti-inflammatory and antimicrobial activities. Compounds 5, 7 and 10a showed minimal ulcerogenic effect and high selectivity towards human recombinant COX-2 over COX-1 enzyme. Their docking outcome correlated with their biological activity and assured the high selectivity binding towards COX-2...
January 4, 2018: Bioorganic Chemistry
https://www.readbyqxmd.com/read/29328809/methods-for-the-control-of-foodborne-pathogens-in-low-moisture-foods
#19
Alma Fernanda Sánchez-Maldonado, Alvin Lee, Jeffrey M Farber
Low-moisture foods (LMFs) have been defined as those food products with a water activity (aw) less than 0.85 and are generally considered less susceptible to microbial spoilage and the growth of foodborne pathogens. However, in recent years, outbreaks linked to LMFs have increased, with Salmonella spp., Bacillus cereus, Cronobacter sakazakii, Clostridium spp., Escherichia coli O157:H7, non-O157 E. coli, and Staphylococcus aureus being the principal pathogens involved. Because of the new concerns raised as a result of recent outbreaks, new approaches need to be developed to control foodborne pathogens in LMFs...
January 12, 2018: Annual Review of Food Science and Technology
https://www.readbyqxmd.com/read/29328653/extending-half-life-of-h-ferritin-nanoparticle-by-fusing-albumin-binding-domain-for-doxorubicin-encapsulation
#20
Chunyue Wang, Chun Zhang, Zenglan Li, Shuang Yin, Qi Wang, Fangxia Guo, Yao Zhang, Rong Yu, Yongdong Liu, Zhiguo Su
Nanoparticles based on the heavy chain of the human ferritin (HFn) are arousing growing interest in the field of drug-delivery due to their exceptional characteristics. However, the unsatisfied plasma half-life of HFn substantially limits its application as a delivery platform for anti-tumor agents. Herein, we fused an albumin binding domain (ABD) variant which basically derives from the streptococcal protein G and possesses a long-acting characteristic in serum albumin to the N-terminus of the HFn for the aim of half-life extension...
January 12, 2018: Biomacromolecules
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