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https://www.readbyqxmd.com/read/29053419/enhanced-sanitation-standard-operating-procedures-have-limited-impact-on-listeria-monocytogenes-prevalence-in-retail-delis
#1
Andrea J Etter, Susan R Hammons, Sherry Roof, Courtenay Simmons, Tongyu Wu, Peter W Cook, Alex Katubig, Matthew J Stasiewicz, Emily Wright, Steven Warchocki, Jill Hollingworth, Hilary S Thesmar, Salam A Ibrahim, Martin Wiedmann, Haley F Oliver
In a recent longitudinal surveillance study in 30 U.S. retail delicatessens, 9.7% of environmental surfaces were positive for Listeria monocytogenes, and we found substantial evidence of persistence. In this study, we aimed to reduce the prevalence and persistence of L. monocytogenes in the retail deli environment by developing and implementing practical and feasible intervention strategies (i.e., sanitation standard operating procedures; SSOPs). These SSOPs were standardized across the 30 delis enrolled in this study...
October 20, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/29045986/impact-of-residency-training-redesign-on-residents-clinical-knowledge
#2
Elaine Waller, M Patrice Eiff, Eve Dexter, Jason C B Rinaldo, Miguel Marino, Roger Garvin, Alan B Douglass, Robert Phillips, Larry A Green, Patricia A Carney
BACKGROUND AND OBJECTIVES: The In-training Examination (ITE) is a frequently used method to evaluate family medicine residents' clinical knowledge. We compared family medicine ITE scores among residents who trained in the 14 programs that participated in the Preparing the Personal Physician for Practice (P4) Project to national averages over time, and according to educational innovations. METHODS: The ITE scores of 802 consenting P4 residents who trained in 2007 through 2011 were obtained from the American Board of Family Medicine...
October 2017: Family Medicine
https://www.readbyqxmd.com/read/29044017/-crispr-cas9-and-gene-therapy
#3
Luciana E Giono
The development of techniques that allow the precise and efficient edition of the genome of living cells is one of the main goals of biomedical research. Over the last few decades, a number of genome editing tools have been developed, the most prominent being the CRISPR/Cas9 system, a bacterial defense mechanism that has been redesigned for its use in other cellular systems. The accessibility, both technical and economical, and the enormous potential of CRISPR/Cas9 have contributed to an almost unprecedented revolution in the biomedical sciences and represent an important step forward in the field of gene therapy that needs, however, to be taken cautiously...
2017: Medicina
https://www.readbyqxmd.com/read/29043470/comprehensive-analysis-of-nmr-data-using-advanced-line-shape-fitting
#4
Markus Niklasson, Renee Otten, Alexandra Ahlner, Cecilia Andresen, Judith Schlagnitweit, Katja Petzold, Patrik Lundström
NMR spectroscopy is uniquely suited for atomic resolution studies of biomolecules such as proteins, nucleic acids and metabolites, since detailed information on structure and dynamics are encoded in positions and line shapes of peaks in NMR spectra. Unfortunately, accurate determination of these parameters is often complicated and time consuming, in part due to the need for different software at the various analysis steps and for validating the results. Here, we present an integrated, cross-platform and open-source software that is significantly more versatile than the typical line shape fitting application...
October 17, 2017: Journal of Biomolecular NMR
https://www.readbyqxmd.com/read/29040681/20-years-of-the-smart-protein-domain-annotation-resource
#5
Ivica Letunic, Peer Bork
SMART (Simple Modular Architecture Research Tool) is a web resource (http://smart.embl.de) for the identification and annotation of protein domains and the analysis of protein domain architectures. SMART version 8 contains manually curated models for more than 1300 protein domains, with approximately 100 new models added since our last update article (1). The underlying protein databases were synchronized with UniProt (2), Ensembl (3) and STRING (4), doubling the total number of annotated domains and other protein features to more than 200 million...
October 10, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29039722/instructional-insights-restructuring-the-fieldwork-experience-one-clinic-s-journey
#6
Sheila Wilson
Although there are standards for occupational therapy education, there are no universal standards for the affiliated fieldwork settings, only guidelines from the educational institutions. As a result, a student learning experience often varies dramatically from facility to facility. Several factors that may affect the clinical experience include the preparedness of a student, the willingness of a clinic to work through potential student challenges, and the theoretical foundation a clinic has built for disseminating occupational therapy practice skills...
October 17, 2017: Occupational Therapy in Health Care
https://www.readbyqxmd.com/read/29038317/change-in-non-alcoholic-beverage-sales-following-a-10-pence-levy-on-sugar-sweetened-beverages-within-a-national-chain-of-restaurants-in-the-uk-interrupted-time-series-analysis-of-a-natural-experiment
#7
Laura Cornelsen, Oliver T Mytton, Jean Adams, Antonio Gasparrini, Dalia Iskander, Cecile Knai, Mark Petticrew, Courtney Scott, Richard Smith, Claire Thompson, Martin White, Steven Cummins
BACKGROUND: This study evaluates changes in sales of non-alcoholic beverages in Jamie's Italian, a national chain of commercial restaurants in the UK, following the introduction of a £0.10 per-beverage levy on sugar-sweetened beverages (SSBs) and supporting activity including beverage menu redesign, new products and establishment of a children's health fund from levy proceeds. METHODS: We used an interrupted time series design to quantify changes in sales of non-alcoholic beverages 12 weeks and 6 months after implementation of the levy, using itemised electronic point of sale data...
November 2017: Journal of Epidemiology and Community Health
https://www.readbyqxmd.com/read/29037112/peer-led-transformative-learning-approaches-increase-classroom-engagement-in-care-self-management-classes-during-inpatient-rehabilitation-of-individuals-with-spinal-cord-injury
#8
Julie Gassaway, Michael L Jones, W Mark Sweatman, Tamara Young
OBJECTIVE: Evaluate effects of revised education classes on classroom engagement during inpatient rehabilitation for individuals with spinal cord injury/disease (SCI/D). DESIGN: Multiple-baseline, quasi-experimental design with video recorded engagement observations during conventional and revised education classes; visual and statistical analysis of difference in positive engagement responses observed in classes using each approach. PARTICIPANTS/SETTING: 81 patients (72% male, 73% white, mean age 36 SD 15...
October 16, 2017: Journal of Spinal Cord Medicine
https://www.readbyqxmd.com/read/29037087/a-label-free-technique-for-accurate-detection-of-nucleic-acid-based-self-avoiding-molecular-recognition-systems-supplemented-multiple-cross-displacement-amplification-and-nanoparticles-based-biosensor
#9
Yi Wang, Yan Wang, Hong Wang, Jianguo Xu, Changyun Ye
Here, we devised a novel isothermal technique on the basis of standard multiple cross-displacement amplification (MCDA), which is assisted with self-avoiding molecular recognition system (SAMRS) components and antarctic thermal-sensitive uracil-DNA-glycosylase enzyme (AUDG), termed AUDG-SAMRS-MCDA. To enable product detection on the dipsticks, we firstly developed an analysis strategy, which did not require the labelled primers or probes, and thus, the analysis system avoids the false-positive results arising from undesired hybridization (between two labelled primers, or the labelled probe and primer)...
October 16, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29034339/a-method-for-the-production-of-large-volumes-of-waf-and-cewaf-for-dosing-mesocosms-to-understand-marine-oil-snow-formation
#10
Terry L Wade, Maya Morales-McDevitt, Gopal Bera, Dawai Shi, Stephen Sweet, Binbin Wang, Gerado Gold-Bouchot, Antonietta Quigg, Anthony H Knap
Marine oil snow (MOS) formation is a mechanism to transport oil from the ocean surface to sediments. We describe here the use of 110L mesocosms designed to mimic oceanic parameters during an oil spill including the use of chemical dispersants in order to understand the processes controlling MOS formation. These experiments were not designed to be toxicity tests but rather to illustrate mechanisms. This paper focuses on the development of protocols needed to conduct experiments under environmentally relevant conditions to examine marine snow and MOS...
October 2017: Heliyon
https://www.readbyqxmd.com/read/29034148/does-a-grill-menu-redesign-influence-sales-nutrients-purchased-and-consumer-acceptance-in-a-worksite-cafeteria
#11
Maya K Vadiveloo, Vasanti S Malik, Donna Spiegelman, Walter C Willett, Josiemer Mattei
Worksite cafeterias are compelling venues to improve diet quality through environmental changes. We conducted a pre-post study to evaluate how a cafeteria-initiated grill menu redesign influenced sales, revenue, and nutrient content of foods purchased. Secondly, we evaluated consumer opinions about menu changes to inform practices for worksite environment interventions. Monthly sales data (2012-2015) were used to compute gross sales and revenue of entrées and side dishes pre-post menu changes. Alternative protein sources replaced red meat; nutrient composition and nutrients purchased were compared using Food Pro software...
December 2017: Preventive Medicine Reports
https://www.readbyqxmd.com/read/29033993/information-processing-in-nursing-information-systems-an-evaluation-study-from-a-developing-country
#12
Mahnaz Samadbeik, Nafiseh Shahrokhi, Marzieh Saremian, Ali Garavand, Mahdi Birjandi
BACKGROUND: In recent years, information technology has been introduced in the nursing departments of many hospitals to support their daily tasks. Nurses are the largest end user group in Hospital Information Systems (HISs). This study was designed to evaluate data processing in the Nursing Information Systems (NISs) utilized in many university hospitals in Iran. METHODS AND MATERIALS: This was a cross-sectional study. The population comprised all nurse managers and NIS users of the five training hospitals in Khorramabad city (N = 71)...
September 2017: Iranian Journal of Nursing and Midwifery Research
https://www.readbyqxmd.com/read/29032349/organizational-home-care-models-across-europe-a-cross-sectional-study
#13
Liza Van Eenoo, Henriëtte van der Roest, Graziano Onder, Harriet Finne-Soveri, Vjenka Garms-Homolova, Palmi V Jonsson, Stasja Draisma, Hein van Hout, Anja Declercq
BACKGROUND: Decision makers are searching for models to redesign home care and to organize health care in a more sustainable way. OBJECTIVES: The aim of this study is to identify and characterize home care models within and across European countries by means of structural characteristics and care processes at the policy and the organization level. DATA SOURCES: At the policy level, variables that reflected variation in health care policy were included based on a literature review on the home care policy for older persons in six European countries: Belgium, Finland, Germany, Iceland, Italy, and the Netherlands...
September 21, 2017: International Journal of Nursing Studies
https://www.readbyqxmd.com/read/29031180/design-of-silk-proteins-with-increased-heme-binding-capacity-and-fabrication-of-silk-heme-materials
#14
Trevor D Rapson, Jian-Wei Liu, Alagacone Sriskantha, Mustafa Musameh, Christopher J Dunn, Jeffrey S Church, Andrea Woodhead, Andrew C Warden, Mark J Riley, Jeffrey R Harmer, Christopher J Noble, Tara D Sutherland
In our previous studies, heme was bound into honeybee silk to generate materials that could function as nitric oxide sensors or as recoverable heterogeneous biocatalysts. In this study, we sought to increase the heme-binding capacity of the silk protein by firstly redesigning the heme binding site to contain histidine as the coordinating residue and secondly, by adding multiple histidine residues within the core of the coiled coil core region of the modified silk protein. We used detergent and a protein denaturant to confirm the importance of the helical structure of the silk for heme coordination...
September 4, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29024977/safer-paediatric-surgical-teams-a-5-year-evaluation-of-crew-resource-management-implementation-and-outcomes
#15
Carl Savage, F Andrew Gaffney, Laith Hussain-Alkhateeb, Pia Olsson Ackheim, Gunilla Henricson, Irini Antoniadou, Mats Hedsköld, Karin Pukk Härenstam
Objective: Evaluate longitudinal changes in technical and non-technical skills (teamwork, situation monitoring, communication and leadership), safety culture, and clinical outcomes before and after implementation of a crew resource management (CRM) safety program. Design: A multi-level prospective single case study in accordance with the SQUIRE-guidelines for reporting quality improvement efforts. Setting: Large university paediatric surgical service...
September 7, 2017: International Journal for Quality in Health Care
https://www.readbyqxmd.com/read/28993287/effectiveness-of-certified-diabetes-educators-following-pre-approved-protocols-to-redesign-diabetes-care-delivery-in-primary-care-results-of-the-remedies-4d-trial
#16
Janice C Zgibor, Maura A Maloney, Markku Malmi, Anthony Fabio, Shihchen Kuo, Francis X Solano, Debra Tilves, Lichuan Tu, Mayer B Davidson
OBJECTIVE: To evaluate the changes in HbA1c, blood pressure, and LDLc levels in participants from practices where certified diabetes educators (CDEs) implemented standardized protocols to intensify treatment compared with those receiveing usual care. RESEARCH DESIGN AND METHODS: This clustered, randomized, clinical trial was implemented in community-based primary care practices. Fifteen primary care practices and 240 patients with type 2 diabetes were randomized to the intervention (n=175) or usual care (n=65)...
October 6, 2017: Contemporary Clinical Trials
https://www.readbyqxmd.com/read/28991437/structural-insight-into-acyl-acp-thioesterase-towards-substrate-specificity-design
#17
Yanbin Feng, Yayue Wang, Jiao Liu, Yinghui Liu, Xupeng Cao, Song Xue
Acyl-ACP thioesterase (TE) catalyzes the hydrolysis of thioester bonds during type II fatty acid synthesis and directly determines fatty acid chain length. Most TEs are responsible for recognition of 16:0 and 18:1 substrates, while specific TEs interrupt acyl-ACP elongation at C8-C14. However, the acyl selection mechanism of TE has not been thoroughly elucidated to date. In this study, the crystal structure of the C12-specific thioesterase FatB from Umbellularia californica, which consists of two independent hotdog domains, was determined...
October 9, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28988568/does-the-accreditation-of-private-dental-practices-work-time-to-rethink-how-accreditation-can-improve-patient-safety
#18
Gillian Jean
Accreditation to demonstrate engagement with the National Safety and Quality Health Service Standards (Standards) is compulsory for most hospital and healthcare settings, but to date remains voluntary for private dental practices (PDPs). The regulatory framework governing the dental profession lacks a proactive element to drive improvements in quality and safety of care, and an accreditation scheme can strengthen existing regulation. The current model of accreditation operating in accordance with the Australian Health Service Safety and Quality Accreditation Scheme (Scheme) is based on the Standards, which were written for a hospital model of healthcare service...
October 9, 2017: Australian Health Review: a Publication of the Australian Hospital Association
https://www.readbyqxmd.com/read/28982829/development-and-evaluation-of-the-ohcities-instrument-assessing-alcohol-urban-environments-in-the-heart-healthy-hoods-project
#19
Xisca Sureda, Albert Espelt, Joan R Villalbí, Alba Cebrecos, Lucía Baranda, Jamie Pearce, Manuel Franco
OBJECTIVES: To describe the development and test-retest reliability of OHCITIES, an instrument characterising alcohol urban environment in terms of availability, promotion and signs of consumption. DESIGN: This study involved: (1) developing the conceptual framework for alcohol urban environment by means of literature reviewing and previous alcohol environment research experience; (2) pilot testing and redesigning the instrument; (3) instrument digitalisation; (4) instrument evaluation using test-retest reliability...
October 5, 2017: BMJ Open
https://www.readbyqxmd.com/read/28982645/workarounds-emerging-from-electronic-health-record-system-usage-consequences-for-patient-safety-effectiveness-of-care-and-efficiency-of-care
#20
Vincent Blijleven, Kitty Koelemeijer, Marijntje Wetzels, Monique Jaspers
BACKGROUND: Health care providers resort to informal temporary practices known as workarounds for handling exceptions to normal workflow unintendedly imposed by electronic health record systems (EHRs). Although workarounds may seem favorable at first sight, they are generally suboptimal and may jeopardize patient safety, effectiveness of care, and efficiency of care. OBJECTIVE: Research into the scope and impact of EHR workarounds on patient care processes is scarce...
October 5, 2017: JMIR Human Factors
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