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Candida albicans biofilm

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https://www.readbyqxmd.com/read/27902776/inhibition-of-candida-albicans-biofilm-formation-by-the-synthetic-lactoferricin-derived-peptide-hlf1-11
#1
Paola Morici, Roberta Fais, Cosmeri Rizzato, Arianna Tavanti, Antonella Lupetti
The aim of this study was to evaluate the in vitro activity of the synthetic peptide hLF1-11 against biofilm produced by clinical isolates of Candida albicans with different fluconazole susceptibility. The antibiofilm activity of the peptide hLF1-11 was assessed in terms of reduction of biofilm cellular density, metabolic activity and sessile cell viability. The extent of morphogenesis in hLF1-11 treated and untreated biofilms was also investigated microscopically. Transcription levels of genes related to cell adhesion, hyphal development and extracellular matrix production were analysed by qRT-PCR in hLF1-11 treated and untreated biofilms...
2016: PloS One
https://www.readbyqxmd.com/read/27900840/peptoid-efficacy-against-polymicrobial-biofilms-determined-by-using-propidium-monoazide-modified-quantitative-pcr
#2
Yu Luo, Hannah L Bolt, Gabriela A Eggimann, Danny F McAuley, Ronan McMullan, Tanya Curran, Mei Zhou, Professor Colin A B Jahoda, Steven L Cobb, Fionnuala T Lundy
Biofilms containing Candida albicans are responsible for a wide variety of clinical infections. The protective effects of the biofilm matrix, the low metabolic activity of microorganisms within a biofilm and their high mutation rate, significantly enhance the resistance of biofilms to conventional antimicrobial treatments. Peptoids are peptide-mimics that share many features of host defence antimicrobial peptides but have increased resistance to proteases and therefore have better stability in vivo. The activity of a library of peptoids was tested against monospecies and polymicrobial bacterial/fungal biofilms...
November 7, 2016: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/27896598/bacterial-and-fungal-biofilm-formation-on-anodized-titanium-alloys-with-fluorine
#3
Concepcion Perez-Jorge, Maria-Angeles Arenas, Ana Conde, Juan-Manuel Hernández-Lopez, Juan-Jose de Damborenea, Steve Fisher, Alessandra M Agostinho Hunt, Jaime Esteban, Garth James
Orthopaedic device-related infections are closely linked to biofilm formation on the surfaces of these devices. Several modified titanium (Ti-6Al-4V) surfaces doped with fluorine were studied in order to evaluate the influence of these modifications on biofilm formation by Gram-positive and Gram-negative bacteria as well as a yeast. The biofilm studies were performed according to the standard test method approved by ASTM (Designation: E2196-12) using the Rotating Disk Reactor. Four types of Ti-6Al-4V samples were tested; chemically polished (CP), two types of nanostructures containing fluorine, nanoporous (NP) and nanotubular (NT), and non-nanostructured fluorine containing samples (fluoride barrier layers, FBL)...
January 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/27888610/camp-independent-signal-pathways-stimulate-hyphal-morphogenesis-in-candida-albicans
#4
Salvatore M Parrino, Haoyu Si, Shamoon Naseem, Kevin Groudan, Justin Gardin, James B Konopka
The fungal pathogen Candida albicans can transition from budding to hyphal growth, which promotes biofilm formation and invasive growth into tissues. Stimulation of adenylyl cyclase to form cAMP induces hyphal morphogenesis. The failure of cells lacking adenylyl cyclase (cyr1Δ) to form hyphae has suggested that cAMP signaling is essential for hyphal growth. However, cyr1Δ mutants also grow slowly and have defects in morphogenesis, making it unclear whether hyphal inducers must stimulate cAMP, or if normal basal levels of cAMP are required to maintain cellular health needed for hyphal growth...
November 26, 2016: Molecular Microbiology
https://www.readbyqxmd.com/read/27879329/phenotypic-profiling-reveals-that-candida-albicans-opaque-cells-represent-a-metabolically-specialized-cell-state-compared-to-default-white-cells
#5
Iuliana V Ene, Matthew B Lohse, Adrian V Vladu, Joachim Morschhäuser, Alexander D Johnson, Richard J Bennett
: The white-opaque switch is a bistable, epigenetic transition affecting multiple traits in Candida albicans including mating, immunogenicity, and niche specificity. To compare how the two cell states respond to external cues, we examined the fitness, phenotypic switching, and filamentation properties of white cells and opaque cells under 1,440 different conditions at 25°C and 37°C. We demonstrate that white and opaque cells display striking differences in their integration of metabolic and thermal cues, so that the two states exhibit optimal fitness under distinct conditions...
November 22, 2016: MBio
https://www.readbyqxmd.com/read/27878654/coating-polypropylene-surfaces-with-protease-weakens-the-adhesion-and-increases-the-dispersion-of-candida-albicans-cells
#6
Eugenio Spadoni Andreani, Federica Villa, Francesca Cappitelli, Anna Krasowska, Piotr Biniarz, Marcin Łukaszewicz, Francesco Secundo
OBJECTIVES: To investigate the ability of the proteases, subtilisin and α-chymotrypsin (aCT), to inhibit the adhesion of Candida albicans biofilm to a polypropylene surface. RESULTS: The proteases were immobilized on plasma-treated polypropylene by covalently linking them with either glutaraldehyde (GA) or N'-diisopropylcarbodiimide (DIC) and N-hydroxysuccinimide (NHS). The immobilization did not negatively affect the enzyme activity and in the case of subtilisin, the activity was up to 640% higher than that of the free enzyme when using N-acetyl phenylalanine ethyl ester as the substrate...
November 23, 2016: Biotechnology Letters
https://www.readbyqxmd.com/read/27870871/functional-genomic-analysis-of-candida-albicans-adherence-reveals-a-key-role-for-the-arp2-3-complex-in-cell-wall-remodelling-and-biofilm-formation
#7
Jason A Lee, Nicole Robbins, Jinglin L Xie, Troy Ketela, Leah E Cowen
Fungal biofilms are complex, structured communities that can form on surfaces such as catheters and other indwelling medical devices. Biofilms are of particular concern with Candida albicans, one of the leading opportunistic fungal pathogens of humans. C. albicans biofilms include yeast and filamentous cells that are surrounded by an extracellular matrix, and they are intrinsically resistant to antifungal drugs such that resolving biofilm infections often requires surgery to remove the contaminated device. C...
November 2016: PLoS Genetics
https://www.readbyqxmd.com/read/27856271/antimicrobial-antitrypanosomal-and-antibiofilm-activity-of-equisetum-hyemale
#8
Camilla Filippi Dos Santos Alves, Pauline Cordenonsi Bonez, Marcia de Ebling de Souza, Ritiel Corrêa da Cruz, Aline Augusti Boligon, Mariana Piana, Thiele Facim Brum, Grazielle Guidolin Rossi, Roberta da Silva Jesus, Thirssa Helena Grando, Silvia Gonzalez Monteiro, Marli Matiko Anraku de Campos, Janice Luering Giongo, Roberto Christ Vianna Santos
This study evaluates, for the first time, the antibiofilm, antimicrobial and antiparasitic potential of crude extract and fractions of stems of Equisetum hyemale against several infectious agents (bacteria, fungi, Mycobacterium and Trypanosomes) by broth microdilution technique and investigates the phenolic composition of the plant by high performance liquid chromatography. The crude extract and fractions showed antimicrobial activity, as they were capable of inhibiting the growth of bacteria in minimal inhibitory concentrations (MICs) ranging from 52...
November 14, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27853317/high-mannose-specific-lectin-msl-mediates-key-interactions-of-the-vaginal-lactobacillus-plantarum-isolate-cmpg5300
#9
Shweta Malik, Mariya I Petrova, Nicole C E Imholz, Tine L A Verhoeven, Sam Noppen, Els J M Van Damme, Sandra Liekens, Jan Balzarini, Dominique Schols, Jos Vanderleyden, Sarah Lebeer
To characterize the interaction potential of the human vaginal isolate Lactobacillus plantarum CMPG5300, its genome was mined for genes encoding lectin-like proteins. cmpg5300.05_29 was identified as the gene encoding a putative mannose-binding lectin. Phenotypic analysis of a gene knock-out mutant of cmpg5300.05_29 showed that expression of this gene is important for auto-aggregation, adhesion to the vaginal epithelial cells, biofilm formation and binding to mannosylated glycans. Purification of the predicted lectin domain of Cmpg5300...
November 17, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27833605/lactobacillus-fermentum-atcc-23271-displays-in-vitro-inhibitory-activities-against-candida-spp
#10
Monique S do Carmo, Francisca M F Noronha, Mariana O Arruda, Ênnio P da Silva Costa, Maria R Q Bomfim, Andrea S Monteiro, Thiago A F Ferro, Elizabeth S Fernandes, Jorge A Girón, Valério Monteiro-Neto
Lactobacilli are involved in the microbial homeostasis in the female genital tract. Due to the high prevalence of many bacterial diseases of the female genital tract and the resistance of microorganisms to various antimicrobial agents, alternative means to control these infections are necessary. Thus, this study aimed to evaluate the probiotic properties of well-characterized Lactobacillus species, including L. acidophilus (ATCC 4356), L. brevis (ATCC 367), L. delbrueckii ssp. delbrueckii (ATCC 9645), L. fermentum (ATCC 23271), L...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27830437/temporal-profile-of-biofilm-formation-gene-expression-and-virulence-analysis-in-candida-albicans-strains
#11
Patrícia Pimentel de Barros, Rodnei Dennis Rossoni, Felipe De Camargo Ribeiro, Juliana Campos Junqueira, Antonio Olavo Cardoso Jorge
The characterization of Candida albicans strains with different degrees of virulence became very useful to understand the mechanisms of fungal virulence. Then, the objective of this study was to assess and compare the temporal profiles of biofilms formation, gene expression of ALS1, ALS3, HWP1, BCR1, EFG1, TEC1, SAP5, PLB2 and LIP9 and virulence in Galleria mellonella of C. albicans ATCC18804 and a clinical sample isolated from an HIV-positive patient (CA60). Although the CFU/mL counting was higher in biofilms formed in vitro by ATCC strain, the temporal profile of the analysis of the transcripts of the C...
November 9, 2016: Mycopathologia
https://www.readbyqxmd.com/read/27820691/bacterial-colonization-in-the-marginal-region-of-ceramic-restorations-effects-of-different-cement-removal-methods-and-polishing
#12
Smb Pereira, L C Anami, C A Pereira, Roa Souza, K Z Kantorski, M A Bottino, Aoc Jorge, L F Valandro
This study evaluated the effects of excess cement removal techniques, with or without subsequent polishing, on biofilm formation and micromorphology in the marginal region of the tooth/restoration. From bovine teeth, 96 dentin blocks (4 × 8 × 2 mm) were produced, molded, and reproduced in type IV gypsum, on which 96 pressed ceramic blocks (Vita PM9, Vita Zahnfabrik; 4 × 8 × 2 mm) were produced via the lost wax technique. The dentin blocks and their respective ceramic blocks were cemented with a self-adhesive resin cement (RelyX U200, 3M ESPE), and cement excess was removed from the margin using four different techniques, followed or not by polishing with silicone rubber tips: MBr, removal with microbrush and photoactivation; MBr-Pol, MBr + polishing; Br, removal with brush and photoactivation; Br-Pol, Br + polishing; Photo-Expl, 5 seconds of initial photoactivation, removal with explorer, and final curing; Photo-Expl-Pol, Photo-Expl + polishing; Photo-SB, 5 seconds of initial photoactivation, removal with scalpel, and final curing; and Photo-SB-Pol, Photo-SB + polishing...
November 2016: Operative Dentistry
https://www.readbyqxmd.com/read/27815078/ziziphora-tenuior-l-essential-oil-from-dana-biosphere-reserve-southern-jordan-chemical-characterization-and-assessment-of-biological-activities
#13
M S Abu-Darwish, C Cabral, M J Gonçalves, C Cavaleiro, M T Cruz, M Paoli, F Tomi, T Efferth, L Salgueiro
ETHNOPHARMACOLOGIC RELEVANCE: Ziziphora tenuior L. (Lamiaceae) is a medicinal plant in Jordan, which is included in various antimicrobial, antiseptic, expectorant and wound healing preparations. It is used for the treatment of cough, stomach ache, dysentery, fever, uterus infection, gut inflammation and painful menstruation. AIM OF THE STUDY: The aim of this study was to assess, for the first time, the chemical composition of the essential oil of Z. tenuior originated from southern Jordan and its antifungal effects against several yeasts...
November 1, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27812615/porosity-of-temporary-denture-soft-liners-containing-antifungal-agents
#14
Jozely Francisca Mello Lima, Janaína Gomes Maciel, Juliana Hotta, Ana Carolina Pero Vizoto, Heitor Marques Honório, Vanessa Migliorini Urban, Karin Hermana Neppelenbroek
Objective: To evaluate the porosity of a tissue conditioner (Softone) and a temporary resilient liner (Trusoft) modified by minimum inhibitory concentrations (MICs) of antifungal agents for Candida albicans biofilm. Material and Methods: The porosity was measured by water absorption, based on exclusion of the plasticizer effect. Initially, it was determined by sorption isotherms that the adequate storage solution for specimens (65×10×3.3 mm) of both materials was 50% anhydrous calcium chloride (S50)...
September 2016: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/27800147/the-role-of-isocitrate-lyase-icl1-in-the-metabolic-adaptation-of-candida-albicans-biofilms
#15
Oluwaseun Ayodeji Ishola, Seng Yeat Ting, Yasser M Tabana, Mowaffaq Adam Ahmed, Muhammad Amir Yunus, Rafeezul Mohamed, Leslie Thian Lung Than, Doblin Sandai
BACKGROUND: A major characteristic of Candida biofilm cells that differentiates them from free-floating cells is their high tolerance to antifungal drugs. This high resistance is attributed to particular biofilm properties, including the accumulation of extrapolymeric substances, morphogenetic switching, and metabolic flexibility. OBJECTIVES: This study evaluated the roles of metabolic processes (in particular the glyoxylate cycle) on biofilm formation, antifungal drug resistance, morphology, and cell wall components...
September 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27796932/the-in-vitro-and-in-vivo-efficacy-of-fluconazole-in-combination-with-farnesol-against-candida-albicans-isolates-using-a-murine-vulvovaginitis-model
#16
Aliz Bozó, Marianna Domán, László Majoros, Gábor Kardos, István Varga, Renátó Kovács
Farnesol is a quorum-sensing molecule that inhibits biofilm formation in Candida albicans. Previous in vitro data suggest that, in combination with certain antifungals, farnesol may have an adjuvant anti-biofilm agent. However, the in vivo efficacy of farnesol is very questionable. Therefore, the in vitro and in vivo activity of fluconazole combined with farnesol was evaluated against C. albicans biofilms using fractional inhibitory concentration index (FICI) determination, time-kill experiments and a murine vulvovaginitis model...
November 2016: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/27795383/screening-the-pathogen-box-for-the-identification-of-candida-albicans-biofilm-inhibitors
#17
Taissa Vila, Jose L Lopez-Ribot
Candida albicans remains the main causative agent of candidiasis, one of the most frequent nosocomial infections, with unacceptably high mortality rates. Biofilm formation is a major risk factor for invasive candidiasis as Candida biofilms display high level resistance to most antifungal agents. In this work we have screened the Pathogen Box® chemical library (Medicines for Malaria Venture - MMV, Switzerland) in search for inhibitors of C. albicans biofilm formation. Our initial screen identified seven hits, and additional dose-response assays confirmed the biofilm-inhibitory activity of six of these small molecules...
October 24, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27793789/inhibition-of-candida-albicans-biofilm-by-pure-selenium-nanoparticles-synthesized-by-pulsed-laser-ablation-in-liquids
#18
Grégory Guisbiers, Humberto H Lara, Ruben Mendoza-Cruz, Guillermo Naranjo, Brandy A Vincent, Xomalin G Peralta, Kelly L Nash
Selenoproteins play an important role in the human body by accomplishing essential biological functions like oxido-reductions, antioxidant defense, thyroid hormone metabolism and immune response; therefore, the possibility to synthesize selenium nanoparticles free of any contaminants is exciting for future nano-medical applications. This paper reports the first synthesis of selenium nanoparticles by femtosecond pulsed laser ablation in de-ionized water. Those pure nanoparticles have been successfully used to inhibit the formation of Candida albicans biofilms...
October 25, 2016: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/27773854/fabrication-of-antimicrobial-silver-doped-carbon-structures-by-combinatorial-pulsed-laser-deposition
#19
Ion N Mihailescu, Dorota Bociaga, Gabriel Socol, George E Stan, Mariana-Carmen Chifiriuc, Coralia Bleotu, Marius A Husanu, Gianina Popescu-Pelin, Liviu Duta, Catalin R Luculescu, Irina Negut, Claudiu Hapenciuc, Cristina Besleaga, Irina Zgura, Florin Miculescu
We report on the selection by combinatorial pulsed laser deposition of Silver-doped Carbon structures with reliable physical-chemical characteristics and high efficiency against microbial biofilms. The investigation of the films was performed by scanning electron microscopy, high resolution atomic force microscopy, energy dispersive X-Ray Spectroscopy, X-ray diffraction, Raman spectroscopy, bonding strength "pull-out" tests, and surface energy measurements. In vitro biological assays were carried out using a large spectrum of bacterial and fungal strains, i...
October 20, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/27771586/antifungal-activity-mode-of-action-and-anti-biofilm-effects-of-laurus-nobilis-linnaeus-essential-oil-against-candida-spp
#20
Larissa Rangel Peixoto, Pedro Luiz Rosalen, Gabriela Lacet Silva Ferreira, Irlan Almeida Freires, Fabíola Galbiatti de Carvalho, Lúcio Roberto Castellano, Ricardo Dias de Castro
OBJECTIVE: The present study demonstrated the antifungal potential of the chemically characterized essential oil (EO) of Laurus nobilis L. (bay laurel) against Candida spp. biofilm adhesion and formation, and further established its mode of action on C. albicans. METHODS: L. nobilis EO was obtained and tested for its minimum inhibitory and fungicidal concentrations (MIC/MFC) against Candida spp., as well as for interaction with cell wall biosynthesis and membrane ionic permeability...
October 18, 2016: Archives of Oral Biology
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