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https://www.readbyqxmd.com/read/29029216/diversity-and-distribution-of-cryptic-species-of-the-bemisia-tabaci-hemiptera-aleyrodidae-complex-in-pakistan
#1
Mariyam Masood, Imran Amin, Ishtiaq Hassan, Shahid Mansoor, Judith K Brown, Rob W Briddon
Bemisia tabaci (Gennadius; Hempitera: Aleyrodidae) is considered to be a cryptic (sibling) species complex, the members of which exhibit morphological invariability while being genetically and behaviorally distinct. Members of the complex are agricultural pests that cause direct damage by feeding on plants, and indirectly by transmitting viruses that cause diseases leading to reduced crop yield and quality. In Pakistan, cotton leaf curl disease, caused by multiple begomovirus species, is the most economically important viral disease of cotton...
October 4, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/29028503/unveiling-the-stimulatory-effects-of-tartrazine-on-human-and-bovine-serum-albumin-fibrillogenesis-spectroscopic-and-microscopic-study
#2
Nasser Abdulatif Al-Shabib, Javed Masood Khan, Mohammad A Alsenaidy, Abdulrahman M Alsenaidy, Mohd Shahnawaz Khan, Fohad Mabood Husain, Mohammad Rashid Khan, Mohammad Naseem, Priyankar Sen, Parvez Alam, Rizwan Hasan Khan
Amyloid fibrils are playing key role in the pathogenesis of various neurodegenerative diseases. Generally anionic molecules are known to induce amyloid fibril in several proteins. In this work, we have studied the effect of anionic food additive dye i.e., tartrazine (TZ) on the amyloid fibril formation of human serum albumins (HSA) and bovine serum albumin (BSA) at pHs7.4 and 3.5. We have employed various biophysical methods like, turbidity measurements, Rayleigh Light Scattering (RLS), Dynamic Light Scattering (DLS), intrinsic fluorescence, Congo red assay, far-UV CD, transmission electron microscopy (TEM) and atomic force microscopy (AFM) to decipher the mechanism of TZ-induce amyloid fibril formation in both the serum albumins at pHs7...
September 22, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29026803/primary-health-care-an-important-approach-for-health-sector-missed-in-iran-s-health-system-evolution-plan
#3
Masoud Behzadifar, Masood Taheri Mirghaed, Aidin Aryankhesal
No abstract text is available yet for this article.
September 2017: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/29026676/spinal-epidural-abscess-report-on-27-cases
#4
Nayil Khursheed, Sultan Dar, Altaf Ramzan, Bashir Fomda, Nisar Humam, Wani Abrar, Sarbjit Singh, Arif Sajad, Masood Mahek, Shoaib Yawar
BACKGROUND: Spinal epidural abscess, although an uncommon disease, often correlates with a high morbidity owing to significant delay in diagnosis. METHODS: In a prospective 5-year study, the clinical and magnetic resonance (MR) findings, treatment protocols, microbiology, and neurological outcomes were analyzed for 27 patients with spinal epidural abscess. RESULTS: Patients were typically middle-aged with underlying diabetes and presented with lumbar abscesses...
2017: Surgical Neurology International
https://www.readbyqxmd.com/read/29026664/supratentorial-extraparenchymal-schwannoma-mimicking-parasagittal-meningioma-a-rare-case-report
#5
Uday S Raswan, Irfan Bhat, Nuzhat Samoon, Sajad H Arif, Masood Laharwal, Sarbjit S Chhiber, Altaf Umar Ramzan
BACKGROUND: Intracranial schwannomas not related to cranial nerves are very rare. Young age, no known history of neurofibromatosis, and seizure as initial symptom have been reported to be associated with intraparenchymal schwannoma. CASE DESCRIPTION: We report a case of supratentorial parasagittal schwannoma in the right frontal region presenting with seizure episode in a 70-year-old man. Computed tomography and magnetic resonance imaging showed a right frontal solid, enhancing extra-axial lesion based on anterior and middle third junction of superior sagittal sinus...
2017: Surgical Neurology International
https://www.readbyqxmd.com/read/29023946/a-novel-recombinant-anti-egfr-peptide-vaccine-capable-of-active-immunization-and-reduction-of-tumor-volume-in-mice-model
#6
Majid Asadi-Ghalehni, Mohamad Javad Rasaee, Masoumeh RajabiBazl, Masood Khosravani, Majid Motaghinejad, Masoud Javanmardi, Saeed Khalili, Helmout Modjtahedi, Esmaeil Sadroddiny
Over-expression of the Epidermal Growth Factor Receptor (EGFR) has been reported in a number of human malignancies. High levels of expression of this receptor have been associated with poor survival in many such patients. Active immunizations that elicit antibodies of the desired type could be an appealing alternative for conventional passive immunization. In this regard, we decide to construct a novel recombinant peptide vaccine capable of prophylactic and therapeutic effects. A novel fusion recombinant peptide base vaccine consisting of L2 domain of murine ECD-EGFR and EGFR mimotope (EM-L2) was constructed and its prophylactic and therapeutic effects on the Lewis lung carcinoma mice (C57/BL6) model were evaluated...
October 10, 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/29020652/negatively-charged-food-additive-dye-allura-red-rapidly-induces-sds-soluble-amyloid-fibril-in-beta-lactoglobulin-protein
#7
Nasser Abdulatif Al-Shabib, Javed Masood Khan, Ajamaluddin Malik, Abdulrahman M Alsenaidy, Mohammad A Alsenaidy, Fohad Mabood Husain, Monis Bilal Shamsi, Syed Hidayathulla, Rizwan Hasan Khan
Recent studies have led to an increased interest to categorize small molecular inhibitors of protein fibrillation. In this study, we used spectroscopy, microscopy and gel electrophoresis techniques that provides an elaborated description of the Allura Red-induced amyloid fibrillation in the β-LG protein at two pHs (7.4 and 3.5). The spectroscopy results show that β-LG protein form aggregates in the presence of Allura Red (0.04 to 15.0mM) at pH 3.5 due to electrostatic and hydrophobic interactions. However, at pH 7...
October 8, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29017667/supervisors-perspective-on-electronic-logbook-system-for-postgraduate-medical-residents-of-cpsp
#8
Khalid Masood Gondal, Uzma Iqbal, Arslan Ahmed, Junaid Sarfraz Khan
OBJECTIVE: To find out the perspective of the supervisors about the role of electronic logbook (E-Logbook) of College of Physicians and Surgeons, Pakistan (CPSP) in monitoring the training of postgraduate medical residents of CPSP. STUDY DESIGN: Descriptive cross-sectional study. PLACE AND DURATION OF STUDY: College of Physicians and Surgeons Pakistan (CPSP), Karachi, from May to October 2015. METHODOLOGY: An electronic computer-based questionnaire designed in Hypertext Preprocessor (PHP) language was distributed to the registered CPSPsupervisors through the e-log system...
September 2017: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://www.readbyqxmd.com/read/28979320/the-effect-of-silver-nanoparticles-on-wounds-contaminated-with-pseudomonas-aeruginosa-in-mice-an-experimental-study
#9
Malahat Ahmadi, Masood Adibhesami
The microorganisms have been usually noted as the major cause of delayed wound healing. Pseudomonas aeruginosa is the most common pathogen causing these infections. Silver nanoparticles (AgNPs) show ample antibacterial activities. In present study, the effect of AgNPs alone and in combination with tetracycline investigated on inoculated wounds with Pseudomonas aeruginosa in mice. Twenty mice anesthetized and full-thickness skin wounds created on back of them and the bacterial suspension added to each wound bed...
2017: Iranian Journal of Pharmaceutical Research: IJPR
https://www.readbyqxmd.com/read/28957691/differential-rubisco-content-and-photosynthetic-efficiency-of-rol-gene-integrated-vinca-minor-transgenic-plant-correlating-factors-associated-with-morpho-anatomical-changes-gene-expression-and-alkaloid-productivity
#10
Priyanka Verma, Shamshad Ahmad Khan, Nusrat Masood, N Manika, Abhishek Sharma, Neha Verma, Suaib Luqman, Ajay K Mathur
Transgenic plants obtained from a hairy root line (PVG) of Vinca minor were characterized in relation to terpenoid indole alkaloids (TIAs) pathway gene expression and vincamine production. The hairy roots formed callus with green nodular protuberances when transferred onto agar-gelled MS medium containing 3.0mg/l zeatin. These meristematic zones developed into shoot buds on medium with 1.0mg/l 2, 4-dichlorophenoxyacetic acid and 40mg/l ascorbic acid. These shoot buds subsequently formed rooted plants when shifted onto a hormone-free MS medium with 6% sucrose...
September 20, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28955088/effects-of-virtual-reality-training-on-mobility-and-physical-function-in-stroke
#11
Arshad Nawaz Malik, Tahir Masood
Stroke is a common disabling condition which declines the functional and mobility level. The purpose of the case series was to determine the effect of virtual reality training on sensorimotor function and mobility level in stroke patients. Ten male (40-60 year) patients of stroke (08 Infarction, 02 Haemorrhagic) were selected from Physiotherapy department of Pakistan Railway Hospital, Rawalpindi. The additional virtual reality training (15-20 minutes) was provided 03 days per week for 06weeks along with task oriented training...
October 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28954252/binding-studies-of-guggulsterone-e-to-calf-thymus-dna-by-multi-spectroscopic-calorimetric-and-molecular-docking-studies
#12
Shoeb Ikhlas, Masood Ahmad
Guggulsterone, a sterol found in plants is used as an ayurvedic medicine for many diseases such as obesity, internal tumors, ulcers etc. E and Z are two isoforms of guggulsterone, wherein guggulsterone-E (GUGE) has also been shown to have anticancer potential. Most of the anticancer drugs target nucleic acids. Therefore, we studied the mode of interaction between ctDNA and GUGE using UV-Vis, fluorescence and CD spectroscopy, isothermal calorimetry along with molecular docking studies. Hoechst 3325, ethidium bromide and rhodamine-B displacement experiments confirms that GUGE binds in the minor groove of DNA...
September 21, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28951301/spider-s-venom-phospholipases-d-a-structural-review
#13
REVIEW
Rehana Masood, Kifayat Ullah, Hamid Ali, Ijaz Ali, Christian Betzel, Anwar Ullah
Spider venoms are complex mixtures of proteins, peptides and small organic and inorganic molecules. Among the proteins, phospholipases D (PLDs) present the major portion, and till now they are the most studied enzymes in spider venom. These PLDs have been divided into two classes, I and II, based on their primary and tertiary structure. Currently, crystal structures of both classes of these enzymes are available in the Protein Data Bank (PDB). Their three-dimensional structure is composed of eight α-helices and eight β-strands forming the ubiquitous fold called triosephosphate isomerase (TIM) barrel...
September 23, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28942500/evaluation-of-immunostimulatory-and-immunotherapeutic-effects-of-tropical-mushroom-lentinus-edodes-against-eimeriasis-in-chicken
#14
Muhammad Irfan Ullah, Masood Akhtar, Mian Muhammad Awais, Muhammad Irfan Anwar, Kashfa Khaliq
Mushrooms (Lentinus edodes) were processed for hot water (HWE), methanolic (ME), and polysaccharide (PSE) extracts. Polysaccharides were isolated through ion exchange (DEAE cellulose) and size exclusion (Sephadex G-100) chromatography. Monosaccharides including maltose (0.282%), glucose (0.113%), and mannose (0.451%) were identified, qualitatively and quantitatively, from the isolated polysaccharides through high-performance liquid chromatography. The whole study was divided into two experiments. Experiment 1 was meant for the evaluation of HWE and ME; whereas, experiment 2 was meant for the evaluation of PSE for immunostimulatory and immunotherapeutic activities...
September 24, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28936377/not-a-common-rectosigmoid-perforation
#15
Rizwan Ishtiaq, Khurram Niaz, Darakshan Masood, Daniyal Ishtiaq, Shafay Yasin, Umer H Chaudhry, Anam Ashraf
Gastrointestinal perforation is a common complication arising due to homicidal injuries, trauma or intake of medications like aspirin. Intestinal perforation caused by chronic intake of mud, clay or soil is a rare phenomenon and very few cases have been reported in the literature. We hereby present the first case of rectosigmoid perforation from Pakistan which was caused by chronic mud intake in a female patient. Diagnosis of this condition in its early stage is important because it can be fatal if not addressed urgently...
July 13, 2017: Curēus
https://www.readbyqxmd.com/read/28934524/associations-of-sleep-duration-and-disturbances-with-hypertension-in-metropolitan-cities-of-delhi-chennai-and-karachi-in-south-asia-cross-sectional-analysis-of-the-carrs-study
#16
Roopa Shivashankar, Dimple Kondal, Mohammed K Ali, Ruby Gupta, Rajendra Pradeepa, Viswanathan Mohan, Muhammad Masood Kadir, K M Venkat Narayan, Nikhil Tandon, Dorairaj Prabhakaran, Anne Peasey
Objectives: Sleep duration and disturbances may be risk factors for hypertension. Despite the high burden of hypertension in South Asia, little is known about this relationship in this region. Methods: We analyzed population-level cross-sectional data from the Centre for Cardiometabolic Risk Reduction in South Asia (CARRS) study that recruited representative samples of adults ≥ 20 years from three cities-Delhi, Chennai (India), and Karachi (Pakistan) during 2010-2011...
September 1, 2017: Sleep
https://www.readbyqxmd.com/read/28932372/acupuncture-and-botulinum-toxin-a-injection-in-the-treatment-of-chronic-migraine-a-randomized-controlled-study
#17
Bahram Naderinabi, Alia Saberi, Masood Hashemi, Mohammad Haghighi, Gelareh Biazar, Farid Abolhasan Gharehdaghi, Abbas Sedighinejad, Tahereh Chavoshi
BACKGROUND: Migraine is a common type of headache. Sometimes adequate pain relief is not achieved by conventional treatments. Acupuncture and botulinum toxin-A injection are known as non-pharmacological interventions for this purpose. The aim of this research was to compare the effect of acupuncture with botulinum toxin-A injection and pharmacological treatment in controlling chronic migraine. METHODS: This clinical trial was conducted on patients with chronic migraine in the North of Iran during 2014-2015...
2017: Caspian Journal of Internal Medicine
https://www.readbyqxmd.com/read/28927258/a-proteomics-based-approach-reveals-differential-regulation-of-visceral-adipose-tissue-proteins-between-metabolically-healthy-and-unhealthy-obese-patients
#18
Assim A Alfadda, Afshan Masood, Mohammed Y Al-Naami, Pierre Chaurand, Hicham Benabdelkamel
Obesity and the metabolic disorders that constitute metabolic syndrome are a primary cause of morbidity and mortality in the world. Nonetheless, the changes in the proteins and the underlying molecular pathways involved in the relevant pathogenesis are poorly understood. In this study a proteomic analysis of the visceral adipose tissue isolated from metabolically healthy and unhealthy obese patients was used to identify presence of altered pathway(s) leading to metabolic dysfunction. Samples were obtained from 18 obese patients undergoing bariatric surgery and were subdivided into two groups based on the presence or absence of comorbidities as defined by the International Diabetes Federation...
September 30, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28924291/varied-percutaneous-and-endovascular-management-of-large-iatrogenic-profunda-femoris-artery-pseudoaneurysm
#19
Wasey Jillani, Raza Sayani, Masood Umer, Muhammad Azeemuddin
The case of an elderly male diagnosed with a large iatrogenic pseudoaneurysm arising from the profunda femoris artery on ultrasound examination is presented. The immediate postoperative period was uneventful; however later he developed leg swelling and mild oozing at the incision site. In view of deranged renal function CT angiogram was not done and decision was made to treat the pseudoaneurysm with percutaneous thrombin injection. Almost complete thrombosis was achieved however a small residual portion remained patent near the neck...
September 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28924287/study-designs-use-of-statistical-tests-and-statistical-analysis-software-choice-in-2015-results-from-two-pakistani-monthly-medline-indexed-journals
#20
Masood Ali Shaikh
Assessment of research articles in terms of study designs used, statistical tests applied and the use of statistical analysis programmes help determine research activity profile and trends in the country. In this descriptive study, all original articles published by Journal of Pakistan Medical Association (JPMA) and Journal of the College of Physicians and Surgeons Pakistan (JCPSP), in the year 2015 were reviewed in terms of study designs used, application of statistical tests, and the use of statistical analysis programmes...
September 2017: JPMA. the Journal of the Pakistan Medical Association
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