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https://www.readbyqxmd.com/read/28938611/dickkopf-1-induces-angiogenesis-via-vegf-receptor-2-regulation-independent-of-the-wnt-signaling-pathway
#1
Sung Hoon Choi, Hyemi Kim, Hyun Gyu Lee, Beom Kyung Kim, Jun Yong Park, Do Young Kim, Sang Hoon Ahn, Kwang-Hyub Han, Seung Up Kim
Tumor angiogenesis is essential for invasive tumor growth and metastasis. Dickkopf-1 (DKK-1), an antagonist of Wnt signaling, participates in tumor development and progression. We evaluated whether DKK-1 stimulation induces angiogenesis and the endothelial-mesenchymal transition (EnMT). Human umbilical vein endothelial cells (HUVECs) were stimulated with recombinant DKK-1 (rDDK-1) or conditioned medium from a culture of DKK-1-transfected 293 cells. Following stimulation, the expression levels of angiogenesis-related factors and EnMT related markers were determined by immunoblot assays...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938544/sialyllactose-suppresses-angiogenesis-by-inhibiting-vegfr-2-activation-and-tumor-progression
#2
Tae-Wook Chung, Eun-Young Kim, Seok-Jo Kim, Hee-Jung Choi, Se Bok Jang, Keuk-Jun Kim, Sun-Hyung Ha, Fukushi Abekura, Choong-Hwan Kwak, Cheorl-Ho Kim, Ki-Tae Ha
The oligosaccharides in human milk have various biological functions. However, the molecular mechanism(s) underlying the anti-angiogenic action of sialylated human milk oligosaccharides (HMOs) are still unclear. Here, we show that siallylactose (SL) found in human milk can inhibit the activation of vascular endothelial growth factor (VEGF)-mediated VEGF receptor-2 (VEGFR-2) by binding to its VEGF binding site (second and third IgG-like domains), thus blocking downstream signal activation. SL also inhibits growth of VEGF-stimulated endothelial cells...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938391/all-internal-segmental-bone-transport-and-optional-lengthening-with-a-newly-developed-universal-cylinder-kombi-tube-module-for-motorized-nails-description-of-a-surgical-technique
#3
Christian Krettek, Ashraf El Naga
Segmental transport is an effective method of treatment for segmental defects, but the need for external fixation during the transport phase is a disadvantage. To avoid external fixation, we have developed a Cylinder-Kombi-Tube Segmental Transport (CKTST) module for combination with a commercially available motorized lengthening nail. This CKTST module allows for an all-internal segmental bone transport and also allows for optional lengthening if needed. The concept and surgical technique of CKTST are described and illustrated with a clinical case...
October 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28938199/determination-of-lead-at-trace-levels-in-mussel-and-sea-water-samples-using-vortex-assisted-dispersive-liquid-liquid-microextraction-slotted-quartz-tube-flame-atomic-absorption-spectrometry
#4
Sezin Erarpat, Gözde Özzeybek, Dotse Selali Chormey, Sezgin Bakırdere
In this study, dispersive liquid-liquid microextraction (DLLME) and slotted quartz tube (SQT) were coupled to flame atomic absorption spectrometry (FAAS) to increase the sensitivity of lead. Conditions such as the formation of the lead-dithizone complex, efficiency of the DLLME method and the output of the SQT were systematically optimized to improve the detection limit for the analyte. The conventional FAAS system was improved upon by about 3.0 times with SQT-FAAS, 32 times with DLLME-FAAS and 142 times with DLLME-SQT-FAAS...
September 17, 2017: Chemosphere
https://www.readbyqxmd.com/read/28938000/podoplanin-increases-the-migration-of-human-fibroblasts-and-affects-the-endothelial-cell-network-formation-a-possible-role-for-cancer-associated-fibroblasts-in-breast-cancer-progression
#5
Jaroslaw Suchanski, Anna Tejchman, Maciej Zacharski, Aleksandra Piotrowska, Jedrzej Grzegrzolka, Grzegorz Chodaczek, Katarzyna Nowinska, Janusz Rys, Piotr Dziegiel, Claudine Kieda, Maciej Ugorski
In our previous studies we showed that in breast cancer podoplanin-positive cancer-associated fibroblasts correlated positively with tumor size, grade of malignancy, lymph node metastasis, lymphovascular invasion and poor patients' outcome. Therefore, the present study was undertaken to assess if podoplanin expressed by fibroblasts can affect malignancy-associated properties of breast cancer cells. Human fibroblastic cell lines (MSU1.1 and Hs 578Bst) overexpressing podoplanin and control fibroblasts were co-cultured with breast cancer MDA-MB-231 and MCF7 cells and the impact of podoplanin expressed by fibroblasts on migration and invasiveness of breast cancer cells were studied in vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28937901/pulp-calcification-in-traumatized-primary-teeth-classification-clinical-and-radiographic-aspects
#6
Anna Carolina Volpi Mello-Moura, Ana Maria Antunes Santos, Gabriela Azevedo Vasconcelos Cunha Bonini, Cristina Giovannetti Del Conte Zardetto, Cacio Moura-Netto, Marcia Turolla Wanderley
OBJECTIVE: The aim of this study was to standardize the nomenclature of pulp alteration to pulp calcification (PC) and to classify it according to type, quantity and location, as well as relate it to clinical and radiographic features. STUDY DESIGN: The dental records of 946 patients from the Research and Clinical Center for Dental Trauma in Primary Teeth were studied. Two hundred and fifty PC-traumatized upper deciduous incisors were detected. RESULTS: According to radiographic analysis of the records, 62...
September 22, 2017: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28937596/synthesis-of-multi-walled-carbon-nanotubes-from-plastic-waste-using-a-stainless-steel-cvd-reactor-as-catalyst
#7
Pranav K Tripathi, Shane Durbach, Neil J Coville
The disposal of non-biodegradable plastic waste without further upgrading/downgrading is not environmentally acceptable and many methods to overcome the problem have been proposed. Herein we indicate a simple method to make high-value nanomaterials from plastic waste as a partial solution to the environmental problem. Laboratory-based waste centrifuge tubes made of polypropylene were chosen as a carbon source to show the process principle. In the process, multi-walled carbon nanotubes (MWCNTs) were synthesized from plastic waste in a two-stage stainless steel 316 (SS 316) metal tube that acted as both reactor vessel and catalyst...
September 22, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28937481/optimization-of-computed-tomography-coronary-angiography-for-improved-plaque-detection
#8
Hany Kashani, Graham Wright, Ali Ursani, Garry Liu, Masoud Hashemi, Narinder S Paul
OBJECTIVE: The study aims to optimize visualization of the coronary wall during computed tomography coronary angiography. METHODS: A coronary plaque phantom was scanned on a wide-volume computed tomography scanner. Spatial resolution, contrast resolution, and vessel wall thickness were measured at different x-ray tube currents and voltages. RESULTS: Spatial resolution ranged from 0.385 to 0.625 mm and was significantly lower at higher currents...
September 20, 2017: Journal of Computer Assisted Tomography
https://www.readbyqxmd.com/read/28937288/pain-and-side-effects-associated-with-4-dimensional-hysterosalpingo-contrast-sonography-for-evaluating-of-the-fallopian-tubes-patency
#9
Hongbo Li, MeiMei Zhang, Ye Qiang, YunFei Ma, ShuXia Mao, Hao Zhang
4-Dimensional hysterosalpingo-contrast sonography (4 D HyCoSy) using SonoVue is regarded as a really good option available for evaluating fallopian tubal patency. This study was designed to assess the pain and adverse effects incurred by women undergoing 4 D HyCoSy. Through evaluating the pelvic pain immediately after 4 D HyCoSy and 30 min after 4 D HyCoSy, the circumstances of the pain relief was also observed. The predictive factors of pain were assessed simultaneously. 827 consecutive women as part of infertility evaluation were included...
September 22, 2017: Computer Assisted Surgery (Abingdon, England)
https://www.readbyqxmd.com/read/28936963/-application-of-self-made-guidance-for-difficult-gastric-tube-placement-in-patients-with-artificial-airway
#10
Xiaojie Chen, Jinchao Zhang, Wenxiu Li, Yiqing Jiang, Lingzhi Lin
OBJECTIVE: To use evidence-based nursing on patients with artificial airway to the practice of stomach tube, and to evaluate the self made guidance for difficult gastric tube placement in patients with artificial airway. METHODS: Forty patients with artificial airway and were difficult to put the tube, and admitted to Department of Critical Care Medicine of Harrison International Peace Hospital Affiliated to Hebei Medical University from April to December in 2016 were enrolled as observation group...
September 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28936962/-evaluation-of-the-effect-of-a-new-fixation-tape-for-tracheal-catheter-in-intensive-care-patients
#11
Limin Chen, Ruihong Liu, Biyu Shen, Lingling Wang, Zhiming Cui, Guanhua Xu
OBJECTIVE: To evaluate the effect of a new type of fixation tape for tracheal catheter in intensive care patients. METHODS: A purposive sampling study was conducted. Ninety patients experienced oral tracheal intubation for mechanical ventilation, and admitted to respiratory intensive care unit (ICU) of the Second Affiliated Hospital of Nantong University from November 2015 to February 2017 were enrolled. All the patients were randomly (random number) divided into the control group and the observation group with 45 patients in each group...
September 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28936951/-preliminary-evaluation-of-sequential-therapy-by-high-flow-nasal-cannula-oxygen-therapy-following-endotracheal-tube-extubation-in-mechanically-ventilated-patients
#12
Zhengfang Zhu, Yuhao Liu, Qixing Wang, Sheng Wang
OBJECTIVE: To evaluate the feasibility and effect of sequential treatment by the heated humidified high flow nasal cannula oxygen therapy (HFNC) in mechanically ventilated patients following endotracheal tube extubation. METHODS: A prospective randomized controlled trial was conducted. Forty-nine patients with the sequential treatment after tracheal intubation extraction admitted to Department of Critical Care Medicine of Shanghai Tenth People's Hospital from January 1st to December 31st 2016 were enrolled...
September 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28936659/transmission-electron-microscopy-tem-to-study-histology-of-pollen-and-pollen-tubes
#13
Peng-Fei Jia, Hong-Ju Li, Wei-Cai Yang
Here, we describe methods of transmission electron microscopy (TEM) based on conventional chemical fixation and high-pressure freezing (HPF) and freeze-substitution (FS) to examine the ultrastructure of Arabidopsis pollen grains and pollen tubes. Compared to other plant samples, such as root or leaf, pollen grains have thick pollen coat and cell wall which limit the permeation of fixative. Thus, it is difficult to obtain high-quality ultrastructural images of pollen. Moreover, pollen tube is very soft and the traditional procedure is too harsh to get an intact pollen tube sample...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936654/imaging-ca-2-dynamics-in-wild-type-and-nadph-oxidase-deficient-mutant-pollen-tubes-with-yellow-cameleon-and-confocal-laser-scanning-microscopy
#14
Christina Maria Franck, Jens Westermann, Aurélien Boisson-Dernier
While cytosolic calcium (Ca(2+)) plays a central role in a myriad of signaling pathways as a secondary messenger, how dynamic changes of cytosolic calcium relate to cell growth control remains poorly understood. The engineering and continuous improvements of genetically encoded calcium sensors such as the Yellow Cameleon (YC) sensors combined with advances in microscopy have allowed imaging with great resolution of the spatiotemporal characteristics of cytosolic [Ca(2+)]cyt in individual cells. An exciting new step consists therefore in cautiously studying calcium dynamics in mutant backgrounds that display disturbed cellular growth behavior to further enhance our understanding on growth-related processes...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936648/live-cell-imaging-of-f-actin-dynamics-during-fertilization-in-arabidopsis-thaliana
#15
Daichi Susaki, Daisuke Maruyama, Ramesh Yelagandula, Frederic Berger, Tomokazu Kawashima
Fertilization comprises a complex series of cellular processes leading to the fusion of a male and female gamete. Many studies have been carried out to investigate each step of fertilization in plants; however, our comprehensive understanding of all the sequential events during fertilization is still limited. This is largely due to difficulty in investigating events in the female gametophyte, which is deeply embedded in the maternal tissue. Recent advances in confocal microcopy assisted by fluorescent marker lines have contributed to visualizing subcellular dynamics in real time during fertilization in vivo...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936612/endotracheal-intubation-ultrasound-guided-versus-fiberscope-in-patients-with-cervical-spine-immobilization
#16
Moustafa Abdelaziz Moustafa, Emad A Arida, Ola M Zanaty, Sameh Fathy El-Tamboly
BACKGROUND: Ultrasound has growing applications in airway management during anesthesia. The aim of the present study was to evaluate the feasibility of real-time ultrasound-guided tracheal intubation in patients with cervical spine immobilization relative to fiberscope-guided tracheal intubation. PATIENTS AND METHODS: This randomized controlled study was carried out on 266 adult patients who have a rigid neck collar in place for cervical spine immobilization and were randomly allocated into two equal groups...
September 21, 2017: Journal of Anesthesia
https://www.readbyqxmd.com/read/28936561/artificial-sensory-organs-latest-progress
#17
REVIEW
Tatsuo Nakamura, Yuji Inada, Keiji Shigeno
This study introduces the latest progress on the study of artificial sensory organs, with a special emphasis on the clinical results of artificial nerves and the concept of in situ tissue engineering. Peripheral nerves have a strong potential for regeneration. An artificial nerve uses this potential to recover a damaged peripheral nerve. The polyglycolic acid collagen tube (PGA-C tube) is a bio-absorbable tube stuffed with collagen of multi-chamber structure that consists of thin collagen films. The clinical application of the PGA-C tube began in 2002 in Japan...
September 21, 2017: Journal of Artificial Organs: the Official Journal of the Japanese Society for Artificial Organs
https://www.readbyqxmd.com/read/28936271/comparison-of-the-volatile-organic-compound-recovery-rates-of-commercial-active-samplers-for-evaluation-of-indoor-air-quality-in-work-environments
#18
Yuichi Miyake, Masahiro Tokumura, Qi Wang, Zhiwei Wang, Takashi Amagai
The Industrial Safety and Health Law in Japan established administrative levels for volatile organic compounds (VOCs) in indoor air. In the present study, these 49 VOCs were extracted from the absorbents of commercial active samplers from Sibata Scientific Technology (carbon-bead active sampler), SKC Inc. (Anasorb CSC sorbent tube), and Gastec (bead-shaped activated carbon tube) using carbon disulfide, and the recovery rates were compared. The VOCs were added to the adsorbents at three concentration levels relative to the administrative levels (×0...
2017: Air Quality, Atmosphere, & Health
https://www.readbyqxmd.com/read/28936174/the-effect-of-velvet-antler-proteins-on-cardiac-microvascular-endothelial-cells-challenged-with-ischemia-hypoxia
#19
Xiang Xiao, Shuqiang Xu, Lin Li, Min Mao, Jinping Wang, Yanjun Li, Ziwei Wang, Fei Ye, Li Huang
Velvet antler (VA) is a precious traditional Chinese medicine that is capable of repeated regeneration. Based on the Chinese medicine theory of coordination the heart and kidneys, VA has been employed to treat heart diseases, including ischemic heart disease, heart failure, and arrhythmia. We examined the effects of VA proteins on primary cardiac microvascular endothelial cells (CMECs) that were subjected to ischemia-hypoxia (IH) to investigate their effects on and mechanism of action in the treatment of ischemic heart disease...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28936148/the-potential-anticoagulant-property-of-caulerpa-lentillifera-crude-extract
#20
Althea R Arenajo, Adrian P Ybañez, Maria Maichol P Ababan, Charlotte E Villajuan, May Rose M Lasam, Chiqui P Young, Julie Lynn A Reyes
BACKGROUND: Anticoagulants have been used in the treatment of several circulatory diseases and thrombotic disorders, and in the blood sampling for hematologic analysis. Sulfated polysaccharides (SP), which have anticoagulant properties, are found in most seaweeds, including Caulerpa spp. OBJECTIVE: The study generally aimed to evaluate the potential anticoagulant property of Caulerpa lentillifera. METHODOLOGY: The whole plant of fresh C. lentillifera was washed thoroughly with distilled water and manually expressed to obtain the extract...
July 2017: International Journal of Health Sciences
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