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https://www.readbyqxmd.com/read/28651342/emissions-of-volatile-odorous-metabolites-by-clostridium-perfringens-in-vitro-study-using-two-broth-cultures
#1
Nishchal K Sharma, Chuck Keerqin, Shu-Biao Wu, Mingan Choct, Robert A Swick
An in vitro study was carried out to investigate the volatile odorous metabolites produced by necrotic enteritis inducing Clostridium perfringens (Cp) type A field strain (EHE-NE18) cultured in two broth media (thioglycollate broth, brain heart infusion broth) at three levels (0, 103 cfu/mL, 106 cfu/mL) using 2 × 3 factorial arrangement of treatments, with each replicated three times. The culture media with and without Cp was freshly prepared on the day of measurement and the culture headspace concentration of odorants was measured using selected ion-flow tube mass spectrometry (SIFT-MS)...
June 23, 2017: Poultry Science
https://www.readbyqxmd.com/read/28651263/quality-and-dose-optimized-ct-trauma-protocol-recommendation-from-a-university-level-i-trauma-center
#2
Johannes Kahn, David Kaul, Georg Böning, Roman Rotzinger, Patrick Freyhardt, Philipp Schwabe, Martin H Maurer, Diane Miriam Renz, Florian Streitparth
Purpose As a supra-regional level-I trauma center, we evaluated computed tomography (CT) acquisitions of polytraumatized patients for quality and dose optimization purposes. Adapted statistical iterative reconstruction [(AS)IR] levels, tube voltage reduction as well as a split-bolus contrast agent (CA) protocol were applied. Materials and Methods 61 patients were split into 3 different groups that differed with respect to tube voltage (120 - 140 kVp) and level of applied ASIR reconstruction (ASIR 20 - 50 %)...
June 26, 2017: RöFo: Fortschritte Auf Dem Gebiete der Röntgenstrahlen und der Nuklearmedizin
https://www.readbyqxmd.com/read/28650807/simultaneous-ultrasound-therapy-and-monitoring-of-microbubble-seeded-acoustic-cavitation-using-a-single-element-transducer
#3
Sophie V Heymans, Christine F Martindale, Andrej Suler, Antonios N Pouliopoulos, Robert J Dickinson, James J Choi
Ultrasound-driven microbubble activity is used in therapeutic applications such as blood clot dissolution and targeted drug delivery. The safety and performance of these technologies are linked to the type and distribution of microbubble activities produced within the targeted area, but controlling and monitoring these activities in vivo and in real-time has proven to be difficult. As therapeutic pulses are often ms-long, microbubble monitoring currently requires a separate transducer used in a passive reception mode...
June 22, 2017: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
https://www.readbyqxmd.com/read/28650726/maternal-health-related-behaviours-during-pregnancy-a-critical-public-health-issue
#4
Aleksandra Górniaczyk, Barbara Czech-Szczapa, Maciej Sobkowski, Karolina Chmaj-Wierzchowska
AIM: The aim of the study was to investigate the health-related behaviours of pregnant women. Maternal health-related habits, use of psychoactive medicines, diet, prevention of neural tube defects, oral cavity hygiene, chronic diseases and physical activity were analysed in a group of pregnant women attending antenatal classes. METHODS: Hundred pregnant women recruited from various antenatal classes in Poznań, Poland, were investigated using a questionnaire based on the Pregnancy Risk Assessment Monitoring System...
June 26, 2017: European Journal of Contraception & Reproductive Health Care
https://www.readbyqxmd.com/read/28650463/type-vi-secretion-tssk-baseplate-protein-exhibits-structural-similarity-with-phage-receptor-binding-proteins-and-evolved-to-bind-the-membrane-complex
#5
Van Son Nguyen, Laureen Logger, Silvia Spinelli, Pierre Legrand, Thi Thanh Huyen Pham, Thi Trang Nhung Trinh, Yassine Cherrak, Abdelrahim Zoued, Aline Desmyter, Eric Durand, Alain Roussel, Christine Kellenberger, Eric Cascales, Christian Cambillau
The type VI secretion system (T6SS) is a multiprotein machine widespread in Gram-negative bacteria that delivers toxins into both eukaryotic and prokaryotic cells. The mechanism of action of the T6SS is comparable to that of contractile myophages. The T6SS builds a tail-like structure made of an inner tube wrapped by a sheath, assembled under an extended conformation. Contraction of the sheath propels the inner tube towards the target cell. The T6SS tail is assembled on a platform-the baseplate-which is functionally similar to bacteriophage baseplates...
June 26, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28650462/haplotype-phasing-of-whole-human-genomes-using-bead-based-barcode-partitioning-in-a-single-tube
#6
Fan Zhang, Lena Christiansen, Jerushah Thomas, Dmitry Pokholok, Ros Jackson, Natalie Morrell, Yannan Zhao, Melissa Wiley, Emily Welch, Erich Jaeger, Ana Granat, Steven J Norberg, Aaron Halpern, Maria C Rogert, Mostafa Ronaghi, Jay Shendure, Niall Gormley, Kevin L Gunderson, Frank J Steemers
Haplotype-resolved genome sequencing promises to unlock a wealth of information in population and medical genetics. However, for the vast majority of genomes sequenced to date, haplotypes have not been determined because of cumbersome haplotyping workflows that require fractions of the genome to be sequenced in a large number of compartments. Here we demonstrate barcode partitioning of long DNA molecules in a single compartment using "on-bead" barcoded tagmentation. The key to the method that we call "contiguity preserving transposition" sequencing on beads (CPTv2-seq) is transposon-mediated transfer of homogenous populations of barcodes from beads to individual long DNA molecules that get fragmented at the same time (tagmentation)...
June 26, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/28650320/combination-of-exendin-4-and-dpp-4-silencing-promoted-angiogenesis-of-human-coronary-artery-endothelial-cells-via-activation-of-pi3k-akt-pathway
#7
Xuewen Qi, Haifeng Liu, Limei Mao, Peng Sun, Degui Kong
This study was aimed to explore the combined effects of Exendin-4 with dipeptidyl peptidase-IV (DPP-4) silencing on vascular endothelial growth factor (VEGF)-induced cell proliferation and angiogenesis in Human Coronary Artery Endothelial Cells (HCAECs), as well as the underlying molecular mechanisms which were involved in this process. HCAECs were treated by exendin-4, small interfering RNA (siRNA) targeting DPP-4 (DPP-4-siRNA) or exendin-4 plus DPP-4-siRNA, respectively. Cell migration, proliferation and angiogenesis in vitro were assessed by scratch-wound assay, MTT, tran swell assay, and matrigel tube formation, respectively...
March 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28650171/comprehensive-lipoprotein-characterization-using-lipidomics-analysis-of-human-plasma
#8
Nicolas Christinat, Mojgan Masoodi
Lipoproteins are responsible for the transport of lipids and other nutrients in the circulation and therefore play an important role in lipid metabolism and dyslipidemia. They have also been linked to multiple metabolic disorders including cardiovascular disease, thus understanding their lipid composition is of crucial importance. Characterization of lipoproteins is a challenging task due to their heterogeneity. In particular, their fractionation is often laborious and time-consuming, making large sets of clinical samples difficult to analyze...
June 26, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28650030/carbon-nanoscroll-silk-crystallite-hybrid-structures-with-controllable-hydration-and-mechanical-properties
#9
Yuan Cheng, Leng-Duei Koh, Fan Wang, Dechang Li, Baohua Ji, Jingjie Yeo, Guijian Guan, Ming-Yong Han, Yong-Wei Zhang
Hybrid structures of nanomaterials (e.g. tubes, scrolls, threads, cages) and biomaterials (e.g. proteins) hold tremendous potential for applications as drug carriers, biosensors, tissue scaffolds, cancer therapeutic agents, etc. However, in many cases, the interacting forces at the nano-bio interfaces and their roles in controlling the structures and dynamics of nano-bio-hybrid systems are very complicated but poorly understood. In this study, we investigate the structure and mechanical behavior of a protein-based hybrid structure, i...
June 26, 2017: Nanoscale
https://www.readbyqxmd.com/read/28649791/bioactivity-and-biomineralization-ability-of-calcium-silicate-based-pulp-capping-materials-after-subcutaneous-implantation
#10
G Hinata, K Yoshiba, L Han, N Edanami, N Yoshiba, T Okiji
AIM: To evaluate the abilities of three calcium silicate-based pulp-capping materials (ProRoot MTA, TheraCal LC, and a prototype tricalcium silicate cement) to produce apatite-like precipitates after being subcutaneously implanted into rats. METHODOLOGY: Polytetrafluoroethylene tubes containing each material were subcutaneously implanted into the backs of Wistar rats. At 7, 14, and 28 days post-implantation, the implants were removed together with the surrounding connective tissue, and fixed in 2...
June 26, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28649488/hemoptysis-in-a-previously-healthy-elderly-patient-with-an-unrecognized-tracheal-bronchus-a-case-report
#11
Moayad Ismail, Paunel Vukasinov, Hung-I Liao, Parvez Mir
A 83-year-old healthy female with no past medical history presented with persistent hemoptysis and respiratory failure. She was found to have a tracheal bronchus on bronchoscopy. Patient underwent pulmonary lavage and sterile irrigation. Patient was stable, but developed excessive hemoptysis which prompted a repeat, emergent, bronchoscopy was performed to advance the ET tube to isolate the left lung from the bleeding right lung. Despite the effort taken, the patient became pulseless from the hypoxia caused by blood spillover into the entire tracheobronchial field...
2017: Respiratory Medicine Case Reports
https://www.readbyqxmd.com/read/28648816/how-light-affects-the-life-of-botrytis
#12
REVIEW
Julia Schumacher
Fungi, like other organisms, actively sense the environmental light conditions in order to drive adaptive responses, including protective mechanisms against the light-associated stresses, and to regulate development. Ecological niches are characterized by different light regimes, for instance light is absent underground, and light spectra from the sunlight are changed underwater or under the canopy of foliage due to the absorption of distinct wavelengths by bacterial, algal and plant pigments. Considering the fact that fungi have evolved to adapt to their habitats, the complexities of their 'visual' systems may vary significantly...
June 22, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28648664/high-dose-ivig-successfully-reduces-high-rhgaa-igg-antibody-titers-in-a-crim-negative-infantile-pompe-disease-patient
#13
Mugdha Rairikar, Zoheb B Kazi, Ankit Desai, Crista Walters, Amy Rosenberg, Priya S Kishnani
Alglucosidase alfa (rhGAA) has altered the course of an otherwise fatal outcome in classic infantile Pompe disease (IPD), which presents with cardiomyopathy and severe musculoskeletal involvement. However, the response to therapy is determined by several factors including the development of high and sustained antibody titers (HSAT) to rhGAA. Cross-reactive immunologic material (CRIM) negative patients are at the highest risk for development of HSAT. Immune tolerance induction (ITI) with methotrexate, rituximab, and intravenous immunoglobulin (IVIG) has been largely successful in preventing the immune response and in achieving tolerance when done in conjunction with enzyme replacement therapy (ERT) initiation...
May 18, 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/28648660/friction-mediates-scission-of-tubular-membranes-scaffolded-by-bar-proteins
#14
Mijo Simunovic, Jean-Baptiste Manneville, Henri-François Renard, Emma Evergren, Krishnan Raghunathan, Dhiraj Bhatia, Anne K Kenworthy, Gregory A Voth, Jacques Prost, Harvey T McMahon, Ludger Johannes, Patricia Bassereau, Andrew Callan-Jones
Membrane scission is essential for intracellular trafficking. While BAR domain proteins such as endophilin have been reported in dynamin-independent scission of tubular membrane necks, the cutting mechanism has yet to be deciphered. Here, we combine a theoretical model, in vitro, and in vivo experiments revealing how protein scaffolds may cut tubular membranes. We demonstrate that the protein scaffold bound to the underlying tube creates a frictional barrier for lipid diffusion; tube elongation thus builds local membrane tension until the membrane undergoes scission through lysis...
June 21, 2017: Cell
https://www.readbyqxmd.com/read/28648613/multiple-mechanisms-of-resistance-to-pyrethroids-in-anopheles-gambiae-s-l-populations-in-niger
#15
H Soumaila, M Idrissa, M Akgobeto, G Habi, H Jackou, I Sabiti, A Abdoulaye, A Daouda, I Souleymane, R Osse
INTRODUCTION: We performed a transversal study to map resistance of malaria vectors (Anopheles mosquitoes) to insecticides in Niger within the frame of the National Malaria Control Program funded by the World Health Organization (WHO). METHOD: Larvae of Anopheles gambiae s.l were collected from November to December 2013 in seven locations selected on the basis of different patterns of use of insecticides and environment. WHO susceptibility test tubes were used on females Anopheles to detect resistance to insecticides...
June 22, 2017: Médecine et Maladies Infectieuses
https://www.readbyqxmd.com/read/28648485/effects-of-straw-phonation-through-tubes-of-varied-lengths-on-sustained-vowels-in-normal-voiced-participants
#16
Randal D Mills, Shawna Rivedal, Colten DeMorett, Grace Maples, Jack J Jiang
OBJECTIVE: To examine the immediate effects of straw phonation exercises in normal subjects while altering the effective length of the vocal tract. STUDY DESIGN: A nonrandomized comparison of semi-occluded vocal tract length during straw phonation exercises was carried out. METHODS: Oral pressure, mean airflow, aerodynamic resistance, and contact quotient were measured in 20 healthy subjects immediately before and after straw phonation exercises...
June 22, 2017: Journal of Voice: Official Journal of the Voice Foundation
https://www.readbyqxmd.com/read/28648364/differentiation-of-human-pluripotent-stem-cells-into-colonic-organoids-via-transient-activation-of-bmp-signaling
#17
Jorge O Múnera, Nambirajan Sundaram, Scott A Rankin, David Hill, Carey Watson, Maxime Mahe, Jefferson E Vallance, Noah F Shroyer, Katie L Sinagoga, Adrian Zarzoso-Lacoste, Jonathan R Hudson, Jonathan C Howell, Praneet Chatuvedi, Jason R Spence, John M Shannon, Aaron M Zorn, Michael A Helmrath, James M Wells
Gastric and small intestinal organoids differentiated from human pluripotent stem cells (hPSCs) have revolutionized the study of gastrointestinal development and disease. Distal gut tissues such as cecum and colon, however, have proved considerably more challenging to derive in vitro. Here we report the differentiation of human colonic organoids (HCOs) from hPSCs. We found that BMP signaling is required to establish a posterior SATB2+ domain in developing and postnatal intestinal epithelium. Brief activation of BMP signaling is sufficient to activate a posterior HOX code and direct hPSC-derived gut tube cultures into HCOs...
June 20, 2017: Cell Stem Cell
https://www.readbyqxmd.com/read/28647813/ghvln4-is-involved-in-cell-elongation-via-regulation-of-actin-organization
#18
Fenni Lv, Mingya Han, Dongdong Ge, Hui Dong, Xiaotong Zhang, Lifeng Li, Peipei Zhang, Zhongqi Zhang, Jing Sun, Kang Liu, Youlu Yuan
GhVLN4 exhibited activity of cross-linking actin filaments into bundles. Overexpression of GhVLN4 increased the abundance of thick actin bundles and resulted in longer cell phenotypes. Actin bundle is a dynamic, higher-order cytoskeleton structure that is essential for cell expansion. Villin is one of the major proteins responsible for crosslinking actin filaments into bundles. However, this kind of actin binding protein has rarely been investigated in cotton. In the present work, a cotton villin gene was molecularly cloned from Upland cotton and denominated as GhVLN4...
June 24, 2017: Planta
https://www.readbyqxmd.com/read/28647716/bilateral-recurrent-pyosalpinx-in-a-sexually-inactive-12-year-old-girl-secondary-to-rare-variant-of-mullerian-duct-anomaly
#19
Tareq Maraqa, Mohamed Mohamed, Daniel Coffey, Gul R Sachwani-Daswani, Crystal Alvarez, Leo Mercer
Pyosalpinx is a severe sequel of chronic pelvic inflammatory disease, whereby the fallopian tubes become filled with pus.(1 2) Pyosalpinx often affects sexually active women and rarely is seen in celibate adolescent girls.(3) We report a case of a 12-year-old girl with no prior sexual history who presented to our emergency department with complaints of severe right lower quadrant pain of 1-day duration. Ultrasonography and CT scan of the abdomen and pelvis revealed free fluid collections in the pelvis without visualisation of the appendix...
June 24, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28647563/in-vitro-comparison-of-holmium-lasers-evidence-for-shorter-fragmentation-time-and-decreased-retropulsion-using-a-modern-variable-pulse-laser
#20
John Roger Bell, Kristina L Penniston, Stephen Y Nakada
OBJECTIVE: To compare the performance of variable and fixed pulse lasers on stone phantoms in vitro. METHODS: Seven millimeter stone phantoms were made to simulate calcium oxalate monohydrate stones using BegoStone Plus. The in vitro setting was created with a clear PVC tube. For each trial, a stone phantom was placed at the open end of the tubing. The Cook Rhapsody H-30 variable pulse laser was tested on both long and short pulse settings and compared to the Dornier H-20 fixed pulse laser, five trials were conducted for each trial arm...
June 21, 2017: Urology
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