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Molecular allergen

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https://www.readbyqxmd.com/read/27921412/impacts-of-thermal-treatments-on-major-and-minor-allergens-of-sea-snail-cerithidea-obtusa-obtuse-horn-shell
#1
Rosmilah Misnan, Norazlin Salahudin Abd Aziz, Zailatul Hani Mohamad Yadzir, Faizal Bakhtiar, Noormalin Abdullah, Shahnaz Murad
Snail is one of the worst causes of food allergy. Thus, the aim of this study was to identify the major and minor allergens of the local marine snail (Cerithidea obtusa) and subsequently to investigate the impacts of heat treatment on the IgE-binding activity of snail allergens. Proteins from raw and heat-treated snails (boiled, roasted and fried) were extracted and then resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Immunoblotting of all extracts were then performed using sera from patients with snail allergy...
August 2016: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/27917622/identification-and-characterization-of-main-allergic-proteins-in-cooked-wolf-herring-fish
#2
Mohsen Mohamadi, Reza Falak, Kobra Mokhtarian, Mohammad Reza Khoramizadeh, Esmaeil Sadroddiny, Gholam Ali Kardar
Our aim in this study was to identify and characterize allergic proteins in cooked wolf herring fish. We heated the crude extract alternatively at 50, 60, 70, 80, 90, and 100°C for one hour and results were compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Also, proteins were immunoblotted with fish-sensitive patients' sera. The major allergenic proteins were identified via mass spectrometry. These allergenic proteins were then purified by anion exchange chromatography and the IgE-immunoreactivity of the fractions was compared with the crude extracts via disk enzyme-linked immunosorbent assay (ELISA)...
October 2016: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/27911466/transparent-nanofibrous-mesh-self-assembled-from-molecular-legos-for-high-efficiency-air-filtration-with-new-functionalities
#3
Varun Kumar Singh, Sai Kishore Ravi, Wanxin Sun, Swee Ching Tan
Alarming levels of particulate matter pollution in air pose a serious health threat in several countries, therefore intriguing a strong need for an economic and a viable technology of air filtration. Current air purification technology is rather expensive with certain types even having the risk of emitting hazardous by-products. The authors have developed a multifunctional air filter inspired from the nasal hairs possessing an ability to specifically trap/exhale the foreign particles and allergens while still letting the air flow...
December 2, 2016: Small
https://www.readbyqxmd.com/read/27887635/anisakis-pegreffii-nematoda-anisakidae-products-modulate-oxidative-stress-and-apoptosis-related-biomarkers-in-human-cell-lines
#4
Concetta Maria Messina, Federica Pizzo, Andrea Santulli, Ivana Bušelić, Mate Boban, Stjepan Orhanović, Ivona Mladineo
BACKGROUND: In countries with elevated prevalence of zoonotic anisakiasis and high awareness of this parasitosis, a considerable number of cases that associate Anisakis sp. (Nematoda, Anisakidae) and different bowel carcinomas have been described. Although neoplasia and embedded larvae were observed sharing the common site affected by chronic inflammation, no association between the nematode and malignancy were directly proved. Similarly, no data are available about the effect of secretory and excretory products of infecting larvae at the host's cellular level, except in respect to allergenic interaction...
November 25, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27873335/lip-b-1-is-a-novel-allergenic-protein-isolated-from-the-booklouse-liposcelis-bostrychophila
#5
O Ishibashi, K Sakuragi, Y Fukutomi, Y Kawakami, Y Kamata, M Sakurai, S Nakayama, H Uchiyama, H Kobayashi, H Kojima, T Inui
BACKGROUND: Booklice, belonging to the order Psocoptera, are small household insect pests that are distributed worldwide. Liposcelis bostrychophila (L. bostrychophila), a common home-inhabiting species of booklouse, infests old books, sheets of paper and stored food. Recent entomological and serological studies demonstrated that L. bostrychophila accounted for the majority of detectable insects in house dust and could be a potent inducer of respiratory allergy. Our recent proteomic analysis identified a potent allergenic protein from L...
November 22, 2016: Allergy
https://www.readbyqxmd.com/read/27864150/effect-of-malondialdehyde-on-the-ovalbumin-structure-and-its-interactions-with-t84-epithelial-cells
#6
Jasna Nikolić, Andrijana Nešić, Milena Čavić, Neda Đorđević, Uroš Anđelković, Marina Atanasković-Marković, Branko Drakulić, Marija Gavrović-Jankulović
BACKGROUND: Protein oxidation can occur as a consequence of lipid peroxidation during food processing. The aim of this work was to explore the effect of malondialdehyde (MDA) modification of ovalbumin (OVA) on its interaction with T84 intestinal cells. METHODS: Molecular dynamics simulation was employed for the prediction of MDA modification in the OVA, while introduced structural changes were evaluated by measurement of carbonyl group content, fluorescence spectra, MS/MS analysis, and IgE reactivity...
November 15, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27853507/developing-food-allergy-a-potential-immunologic-pathway-linking-skin-barrier-to-gut
#7
REVIEW
Yui-Hsi Wang
Immunoglobulin E (IgE)-mediated food allergy is an adverse reaction to foods and is driven by uncontrolled type-2 immune responses. Current knowledge cannot explain why only some individuals among those with food allergy are prone to develop life-threatening anaphylaxis. It is increasingly evident that the immunologic mechanisms involved in developing IgE-mediated food allergy are far more complex than allergic sensitization. Clinical observations suggest that patients who develop severe allergic reactions to food are often sensitized through the skin in early infancy...
2016: F1000Research
https://www.readbyqxmd.com/read/27845281/patterns-of-sensitization-by-food-and-inhalant-components-in-georgian-patients
#8
N Lomidze, M Gotua
Molecular-based allergy (MA) diagnostics is a novel approach used to map the allergen sensitization of a patient at a molecular level, using purified natural or recombinant allergen components instead of allergen extracts. The aim of our study was to: 1) investigate sensitization patterns to food and inhalant allergen components in different age groups of Georgian allergic patients 2) evaluate patient's potential risk of systemic reaction and manage dietary modifications. 3) Study food and inhalant components in different age groups in correlation with clinical manifestations of allergic disease...
October 2016: Georgian Medical News
https://www.readbyqxmd.com/read/27826019/molecular-cloning-expression-and-ige-immunoreactivity-of-phospholipase-a1-a-major-allergen-from-polybia-paulista-hymenoptera-vespidae-venom
#9
Amilcar Perez-Riverol, Franco Dani Campos Pereira, Alexis Musacchio Lasa, Luis Gustavo Romani Fernandes, José Roberto Aparecido Dos Santos-Pinto, Débora Lais Justo-Jacomini, Gabriel Oliveira de Azevedo, Murilo Luiz Bazon, Mario Sergio Palma, Ricardo de Lima Zollner, Márcia Regina Brochetto-Braga
Polybia paulista (Hymenoptera: Vespidae) is a clinically relevant social wasp that frequently causes stinging accidents in southeast Brazil. To date, diagnosis and specific immunotherapy (SIT) of allergy are based on the use of crude venom extracts. Production of recombinant forms of major allergens from P. paulista venom will improve diagnosis and SIT of allergic patients by reducing the incidence of cross-reactivity and non-specific sensitization. Here, we describe the molecular cloning, heterologous expression, purification and IgE-mediated immunodetection of phospholipase A1 (Poly p 1), a major allergen from P...
December 15, 2016: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/27820941/functions-of-exosomes-and-microbial-extracellular-vesicles-in-allergy-and-contact-and-delayed-type-hypersensitivity
#10
Katarzyna Nazimek, Krzysztof Bryniarski, Philip W Askenase
Extracellular vesicles, such as exosomes, are newly recognized intercellular conveyors of functional molecular mechanisms. Notably, they transfer RNAs and proteins between different cells that can then participate in the complex pathogenesis of allergic and related hypersensitivity responses and disease mechanisms, as described herein. This review highlights this important new appreciation of the in vivo participation of such extracellular vesicles in the interactions between allergy-mediating cells. We take into account paracrine epigenetic exchanges mediated by surrounding stromal cells and the endocrine receipt of exosomes from distant cells via the circulation...
2016: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/27818326/an-update-on-lymphocyte-subtypes-in-asthma-and-airway-disease
#11
REVIEW
Daniel M Moldaver, Mark Larché, Christopher D Rudulier
Inflammation is a hallmark of many airway diseases. Improved understanding of the cellular and molecular mechanisms of airway disease will facilitate the transition in our understanding from phenotypes to endotypes, thereby improving our ability to target treatments based on pathophysiology. For example, allergic asthma has long been considered to be driven by an allergen-specific Th2 response. However, clinical and mechanistic studies have begun to shed light on the role of other cell subsets in the pathogenesis and regulation of lung inflammation...
November 3, 2016: Chest
https://www.readbyqxmd.com/read/27818052/scaffolds-for-oriented-and-close-packed-immobilization-of-immunoglobulins
#12
REVIEW
Masumi Iijima, Shun'ichi Kuroda
Immunosensing is a widely used technique that detects the interactions between antibodies and antigens such as biochemical markers, pathogens, allergens, and tumor-associated antigens. Since target antigens are often of high molecular mass and their binding affinities are sometimes weak, the spatial arrangement of immunoglobulin Gs (IgGs) on immunosensing probes should be optimized by presenting them in as close-packed a manner as possible and reducing steric hindrance around the antigen-binding Fv regions...
October 5, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27816475/proteomics-analysis-of-dendritic-cell-activation-by-contact-allergens-reveals-possible-biomarkers-regulated-by-nrf2
#13
Franz Mussotter, Janina Melanie Tomm, Zeina El Ali, Marc Pallardy, Saadia Kerdine-Römer, Mario Götz, Martin von Bergen, Andrea Haase, Andreas Luch
Allergic contact dermatitis is a widespread disease with high clinical relevance affecting approximately 20% of the general population. Typically, contact allergens are low molecular weight electrophilic compounds which can activate the Keap1/Nrf2 pathway. We performed a proteomics study to reveal possible biomarkers for dendritic cell (DC) activation by contact allergens and to further elucidate the role of Keap1/Nrf2 signaling in this process. We used bone marrow derived dendritic cells (BMDCs) of wild-type (nrf2(+/+)) and Nrf2 knockout (nrf2(-/-)) mice and studied their response against the model contact sensitizers 2,4-dinitrochlorobenzene (DNCB), cinnamaldehyde (CA) and nickel(II) sulfate by 2-dimensional polyacrylamide gel electrophoresis (2D-PAGE) in combination with electrospray ionization tandem mass spectrometry (ESI-MS/MS)...
December 15, 2016: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/27795547/food-allergy-immune-mechanisms-diagnosis-and-immunotherapy
#14
Wong Yu, Deborah M Hussey Freeland, Kari C Nadeau
Food allergy is a pathological, potentially deadly, immune reaction triggered by normally innocuous food protein antigens. The prevalence of food allergies is rising and the standard of care is not optimal, consisting of food-allergen avoidance and treatment of allergen-induced systemic reactions with adrenaline. Thus, accurate diagnosis, prevention and treatment are pressing needs, research into which has been catalysed by technological advances that are enabling a mechanistic understanding of food allergy at the cellular and molecular levels...
December 2016: Nature Reviews. Immunology
https://www.readbyqxmd.com/read/27789064/allergenicity-of-vertebrate-tropomyosins-challenging-an-immunological-dogma
#15
J González-Fernández, A Daschner, C Cuéllar
With the exception of tilapia tropomyosin, other anecdotic reports of tropomyosin recognition of vertebrate origin are generally not accompanied by clinical significance and a dogmatic idea is generally accepted about the inexistence of allergenicity of vertebrate tropomyosins, based mainly on sequence similarity evaluations with human tropomyosins. Recently, a specific work-up of a tropomyosin sensitised patient with seafood allergy, demonstrated that the IgE-recognition of tropomyosin from different fish species can be clinically relevant...
October 24, 2016: Allergologia et Immunopathologia
https://www.readbyqxmd.com/read/27788883/urtica-dioica-pollen-allergy-clinical-biological-and-allergomics-analysis
#16
Angelica Tiotiu, Andrea Brazdova, Cyril Longé, Patrice Gallet, Martine Morisset, Virginie Leduc, Christiane Hilger, Cédric Broussard, Rémy Couderc, Jean-Pierre Sutra, Hélène Sénéchal, Pascal Poncet
BACKGROUND: The most emblematic members of Urticaceae at allergic risk level are wall pellitories (Parietaria), whereas nettle (Urtica) pollen is considered as poorly allergenic. No allergen from nettle pollen has yet been characterized, whereas 4 are listed for Parietaria pollen by the International Union of Immunological Societies. Clinical and biological profiles of 2 adult men who developed symptoms against nettle pollen and/or leaves were studied. OBJECTIVE: To characterize the allergic reaction and identify the potential nettle pollen sensitizing allergens...
November 2016: Annals of Allergy, Asthma & Immunology
https://www.readbyqxmd.com/read/27787580/-insect-venom-allergies-update-2016-for-otorhinolaryngologists
#17
L Klimek, N Dippold, A Sperl
Due to the increasing incidence of hymenoptera venom allergies and the potentially life-threatening reactions, it is important for otolaryngologists working in allergology to have an understanding of modern diagnostic and treatment standards for this allergic disease. Molecular diagnosis with recombinant single allergens from bee and wasp venom components improves the diagnostics of insect venom allergies, particularly in patients with double-positive extract-based test results. Detection of specific sensitizations to bee or wasp venom enables double sensitizations to be better distinguished from cross-reactivity...
December 2016: HNO
https://www.readbyqxmd.com/read/27784212/plant-pathogenesis-related-proteins-pr-10-and-pr-14-as-components-of-innate-immunity-system-and-ubiquitous-allergens
#18
Tatiana V Ovchinnikova, Ekaterina I Finkina, Daria N Melnikova, Ivan V Bogdanov
Pathogenesis-related (PR) proteins are components of innate immunity system in plants. They play an important role in plant defense against pathogens. Lipid transfer proteins (LTPs) and Bet v 1 homologues comprise two separate families of PR-proteins. Both LTPs (PR-14) and Bet v 1 homologues (PR-10) are multifunctional small proteins involving in plant response to abiotic and biotic stress conditions. The representatives of these PR-protein families do not show any sequence similarity but have other common biochemical features such as low molecular masses, the presence of hydrophobic cavities, ligand binding properties, and antimicrobial activities...
October 26, 2016: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/27755183/allergen-immunotherapy-for-the-polyallergic-patient
#19
Ulrich Wahn
PURPOSE OF REVIEW: Currently, dramatic changes in allergy treatment can be observed in many countries: The availability of monocomponent products with pharmaceutical quality for sublingual immunotherapy is likely to have a strong impact on all immunotherapy practices and the specialty in general. The market for patient-named products with allergenic mixtures can be expected to shrink. Allergists are concerned about the possibility that single-source products for immunotherapy might be insufficient to serve the needs of all 'polyallergic' patients...
December 2016: Current Opinion in Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27725242/constituents-from-oak-bark-quercus-robur-l-inhibit-degranulation-and-allergic-mediator-release-from-basophils-and-mast-cells-in-vitro
#20
Peter Lorenz, Miriam Heinrich, Manuel Garcia-Käufer, Franziska Grunewald, Silke Messerschmidt, Anja Herrick, Kim Gruber, Christiane Beckmann, Matthias Knoedler, Roman Huber, Carmen Steinborn, Florian C Stintzing, Carsten Gründemann
ETHNOPHARMACOLOGICAL RELEVANCE: Oak bark has been used since ancient times in Europaen ethnomedicine because of its adstringent, antimicrobial and hemostatic features, e.g. as a remedy for the treatment of wounds and skin diseases. PURPOSE: Oak bark tannins are considered as bioactive natural products, interacting with surface proteins of mucous membranes and might be beneficial for the treatment of allergic diseases. This study investigated the effect of an oak bark decoction (OBD) and isolated tannin fractions on the degranulation capacity and cytokine/chemokine release from rat basophilic cells and human mast cells in vitro, which are essential for the initiation of early- and late-phase allergic reactions...
October 7, 2016: Journal of Ethnopharmacology
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