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Molecular allergen

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https://www.readbyqxmd.com/read/28516510/proteomic-analysis-of-lupinus-angustifolius-var-zeus-and-bojar-and-lupinus-luteus-var-lord-and-parys-seed-proteins-and-their-hydrolysates
#1
Jaroslaw Czubinski, Magdalena Montowska, Edward Pospiech, Eleonora Lampart-Szczapa
BACKGROUND: Proteins enzymatic digestion is a very complex process, during which some components are degraded, while others remain in an unchanged form. Moreover, the enzymatic hydrolysis is one of the most popular methods used to reduce the allergenicity of food proteins. In this study, the efficiency of enzymatic hydrolysis of lupin seeds proteins was assessed by proteomic analysis performed by two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry identification...
May 18, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28507730/application-of-the-adverse-outcome-pathway-aop-concept-to-structure-the-available-in-vivo-and-in-vitro-mechanistic-data-for-allergic-sensitization-to-food-proteins
#2
REVIEW
Jolanda H M van Bilsen, Edyta Sienkiewicz-Szłapka, Daniel Lozano-Ojalvo, Linette E M Willemsen, Celia M Antunes, Elena Molina, Joost J Smit, Barbara Wróblewska, Harry J Wichers, Edward F Knol, Gregory S Ladics, Raymond H H Pieters, Sandra Denery-Papini, Yvonne M Vissers, Simona L Bavaro, Colette Larré, Kitty C M Verhoeckx, Erwin L Roggen
BACKGROUND: The introduction of whole new foods in a population may lead to sensitization and food allergy. This constitutes a potential public health problem and a challenge to risk assessors and managers as the existing understanding of the pathophysiological processes and the currently available biological tools for prediction of the risk for food allergy development and the severity of the reaction are not sufficient. There is a substantial body of in vivo and in vitro data describing molecular and cellular events potentially involved in food sensitization...
2017: Clinical and Translational Allergy
https://www.readbyqxmd.com/read/28503403/diagnostics-in-hymenoptera-venom-allergy-current-concepts-and-developments-with-special-focus-on-molecular-allergy-diagnostics
#3
Thilo Jakob, David Rafei-Shamsabadi, Edzard Spillner, Sabine Müller
BACKGROUND: The high rate of asymptomatic sensitization to Hymenoptera venom, difficulty in correctly identifying Hymenoptera and loss of sensitization over time make an accurate diagnosis of Hymenoptera venom allergy challenging. Although routine diagnostic tests encompassing skin tests and the detection of venom-specific IgE antibodies with whole venom preparations are reliable, they offer insufficient precision in the case of double sensitized patients or in those with a history of sting anaphylaxis, in whom sensitization cannot be proven or only to the presumably wrong venom...
2017: Allergo Journal International
https://www.readbyqxmd.com/read/28502749/a-relevant-ige-reactive-28kda-protein-identified-from-salsola-kali-pollen-extract-by-proteomics-is-a-natural-degradation-product-of-an-integral-47kda-polygalaturonase
#4
Salvador Mas, Carmen Oeo-Santos, Javier Cuesta-Herranz, Araceli Díaz-Perales, Carlos Colás, Javier Fernández, Domingo Barber, Rosalía Rodríguez, V de Los Ríos, Rodrigo Barderas, Mayte Villalba
A highly prevalent IgE-binding protein band of 28kDa is observed when Salsola kali pollen extract is incubated with individual sera from Amaranthaceae pollen sensitized patients. By an immunoproteomic analysis of S. kali pollen extract, we identified this protein band as an allergenic polygalacturonase enzyme. The allergen, named Sal k 6, exhibits a pI of 7.14 and a molecular mass of 39,554.2Da. It presents similarities to Platanaceae, Poaceae, and Cupressaceae allergenic polygalacturonases. cDNA-encoding sequence was subcloned into the pET41b vector and produced in bacteria as a His-tag fusion recombinant protein...
May 10, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28502519/-blomia-tropicalis-a-house-dust-mite-in-the-tropics
#5
REVIEW
L Guilleminault, C Viala-Gastan
INTRODUCTION: Blomia tropicalis is a mite that belongs to the superfamily of Glycyphagidae. Initially described as a storage mite, it is now considered as a house dust mite of tropical and sub-tropical areas. STATE OF THE ART: Sensitization to this mite is very common in South America and Southeast Asia. Epidemiological studies have also found sensitization to this mite in Africa and Central America. Blo t 5 is the major allergen of B. tropicalis. Co-sensitization to other house dust mites such as Dermatophagoides pteronyssinus (Der p) and Dermatophagoides farinae (Der f) is very common...
May 12, 2017: Revue des Maladies Respiratoires
https://www.readbyqxmd.com/read/28496122/structural-insights-into-a-20-8-kda-tegumental-allergen-like-tal-protein-from-clonorchis-sinensis
#6
Chang Hwa Jo, Jonghyeon Son, Sulhee Kim, Takashi Oda, Jaehoon Kim, Myoung-Ro Lee, Mamoru Sato, Hyun Tae Kim, Satoru Unzai, Sam-Yong Park, Kwang Yeon Hwang
Survival of Clonorchis sinensis, a cause of human clonorchiasis, requires tegument proteins, which are localized to the tegumental outer surface membrane. These proteins play an important role in a host response and parasite survival. Thus, these proteins are interesting molecular targets for vaccine and drug development. Here, we have determined two crystal structures of the calmodulin like domain (amino acid [aa] positions 1-81) and dynein light chain (DLC)-like domain (aa 83-177) of a 20.8-kDa tegumental-allergen-like protein from Clonorchis sinensis (CsTAL3)...
May 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28494206/component-resolved-evaluation-of-the-content-of-major-allergens-in-therapeutic-extracts-for-specific-immunotherapy-of-honeybee-venom-allergy
#7
Simon Blank, Stefanie Etzold, Ulf Darsow, Maximilian Schiener, Bernadette Eberlein, Dennis Russkamp, Sara Wolf, Anke Graessel, Tilo Biedermann, Markus Ollert, Carsten B Schmidt-Weber
Allergen-specific immunotherapy is the only curative treatment for honeybee venom (HBV) allergy, which is able to protect against further anaphylactic sting reactions. Recent analyses on a molecular level have demonstrated that HBV represents a complex allergen source that contains more relevant major allergens than formerly anticipated. Moreover, allergic patients show very diverse sensitization profiles with the different allergens. HBV-specific immunotherapy is conducted with HBV extracts which are derived from pure venom...
May 11, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28479600/a-crucial-role-of-the-pd-1h-coinhibitory-receptor-in-suppressing-experimental-asthma
#8
Huafeng Liu, Xin Li, Li Hu, Min Zhu, Bailin He, Liqun Luo, Lieping Chen
Programmed death one homolog (PD-1H) is a cell surface molecule of the B7/CD28 immune modulatory gene family. Although PD-1H has been shown to function as a coinhibitory receptor on T cells to limit naive T-cell activation and proliferation, its role in the regulation of the T-cell response to allergens is unknown. We report here that genetic ablation or blockade of PD-1H drastically promotes pulmonary inflammation with massive accumulation of eosinophils in a mouse model of experimental asthma, indicating a suppressive function of PD-1H in allergic inflammation...
May 8, 2017: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/28479335/allergen-specific-igg-antibodies-signaling-via-fc%C3%AE-riib-promote-food-tolerance
#9
Oliver T Burton, Jaciel M Tamayo, Amanda J Stranks, Kyle J Koleoglou, Hans C Oettgen
BACKGROUND: Food-allergic subjects produce high-titer IgE antibodies that bind to mast cells via FcεRI and trigger immediate hypersensitivity reactions upon antigen encounter. Food-specific IgG antibodies arise in the setting of naturally resolving food allergy and accompany the acquisition of food allergen unresponsiveness in oral immunotherapy (OIT). OBJECTIVE: In this study, we sought to delineate the effects of IgG and its inhibitory Fc receptor, FcγRIIb, on both de novo allergen sensitization in naïve animals and on established immune responses in the setting of pre-existing food allergy...
May 4, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28479330/glycans-and-glycan-specific-ige-in-clinical-and-molecular-allergology-sensitization-diagnostics-and-clinical-symptoms
#10
Arne Homann, Gabriele Schramm, Uta Jappe
Glycan-specific IgE antibodies are cross-reacting with highly similar or even identical carbohydrate structures on a variety of different natural allergens, the so-called cross-reactive carbohydrate determinants (CCDs). In clinical practice, CCDs often interfere with the specificity of in vitro allergy diagnostics, thus impairing allergy therapy decisions for individual patients. Strikingly, these IgE antibodies directed against CCD often do not cause clinically relevant allergy symptoms. Opposed to that, the IgE-binding glycan allergen galactose-α-(1,3)-galactose (α-Gal) is associated with IgE-mediated delayed anaphylaxis in meat allergy...
May 4, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28472641/recombinant-allergy-vaccines-based-on-allergen-derived-b-cell-epitopes
#11
REVIEW
Rudolf Valenta, Raffaela Campana, Verena Niederberger
Immunoglobulin E (IgE)-associated allergy is the most common immunologically-mediated hypersensitivity disease. It affects more than 25% of the population. In IgE-sensitized subjects, allergen encounter can causes a variety of symptoms ranging from hayfever (allergic rhinoconjunctivitis) to asthma, skin inflammation, food allergy and severe life-threatening anaphylactic shock. Allergen-specific immunotherapy (AIT) is based on vaccination with the disease-causing allergens. AIT is an extremely effective, causative and disease-modifying treatment...
May 1, 2017: Immunology Letters
https://www.readbyqxmd.com/read/28463786/innate-immunity-as-the-orchestrator-of-allergic-airway-inflammation-and-resolution-in-asthma
#12
REVIEW
Despoina Thiriou, Ioannis Morianos, Georgina Xanthou, Konstantinos Samitas
The respiratory system is constantly in direct contact with the environment and, has therefore, developed strong innate and adaptive immune responses to combat pathogens. Unlike adaptive immunity which is mounted later in the course of the immune response and is naive at the outset, innate immunity provides the first line of defense against microbial agents, while also promoting resolution of inflammation. In the airways, innate immune effector cells mainly consist of eosinophils, neutrophils, mast cells, basophils, macrophages/monocytes, dendritic cells and innate lymphoid cells, which attack pathogens directly or indirectly through the release of inflammatory cytokines and antimicrobial peptides, and coordinate T and B cell-mediated adaptive immunity...
April 29, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28455109/bronchoprotective-mechanisms-for-specialized-pro-resolving-mediators-in-the-resolution-of-lung-inflammation
#13
REVIEW
Melody G Duvall, Thayse R Bruggemann, Bruce D Levy
Bronchi are exposed daily to irritants, microbes and allergens as well as extremes of temperature and acid. The airway mucosal epithelium plays a pivotal role as a sentinel, releasing alarmins when danger is encountered. To maintain homeostasis, an elaborate counter-regulatory network of signals and cellular effector mechanisms are needed. Specialized pro-resolving mediators (SPMs) are chemical mediators that enact resolution programs in response to injury, infection or allergy. SPMs are enzymatically derived from essential polyunsaturated fatty acids with potent cell-type specific immunoresolvent properties...
May 17, 2017: Molecular Aspects of Medicine
https://www.readbyqxmd.com/read/28453304/mold-allergy-is-it-real-and-what-do-we-do-about-it
#14
Amanda Rudert, Jay Portnoy
fungi produce substances that contain pathogen-associated molecular patterns (pamps) and damage-associated molecular patterns (damps) which bind to pattern recognition receptors, stimulating innate immune responses in humans. they also produce allergens that induce production of specific ige. Areas covered: In this review we cover both innate and adaptive immune responses to fungi. Some fungal products can activate both innate and adaptive responses and in doing so, cause an intense and complex health effects...
May 17, 2017: Expert Review of Clinical Immunology
https://www.readbyqxmd.com/read/28451054/molecular-fingerprinting-of-complex-grass-allergoids-size-assessments-reveal-new-insights-in-epitope-repertoires-and-functional-capacities
#15
S Starchenka, A J Bell, J Mwange, M A Skinner, M D Heath
BACKGROUND: Subcutaneous allergen immunotherapy (SCIT) is a well-documented treatment for allergic disease which involves injections of native allergen or modified (allergoid) extracts. The use of allergoid vaccines is a growing sector of the allergy immunotherapy market, associated with shorter-course therapy. The aim of this study was the structural and immunological characterisation of group 1 (Lol p 1) IgG-binding epitopes within a complex mix grass allergoid formulation containing rye grass...
2017: World Allergy Organization Journal
https://www.readbyqxmd.com/read/28434865/allergens-involved-in-the-cross-reactivity-of-aedes-aegypti-with-other-arthropods
#16
Jose Fernando Cantillo, Leonardo Puerta, Sylvie Lafosse-Marin, Jose Luis Subiza, Luis Caraballo, Enrique Fernandez-Caldas
BACKGROUND: Cross-reactivity between Aedes aegypti and mites, cockroaches, and shrimp has been previously suggested, but the involved molecular components have not been fully described. OBJECTIVE: To evaluate the cross-reactivity between A aegypti and other arthropods. METHODS: Thirty-four serum samples from patients with asthma and/or allergic rhinitis were selected, and specific IgE to A aegypti, Dermatophagoides pteronyssinus, Dermatophagoides farinae, Blomia tropicalis, Periplaneta americana...
April 20, 2017: Annals of Allergy, Asthma & Immunology
https://www.readbyqxmd.com/read/28428126/interactions-of-tetracyclines-with-ovalbumin-the-main-allergen-protein-from-egg-white-spectroscopic-and-electrophoretic-studies
#17
Maria Dayanne de A Dantas, Humberto de Araújo Tenório, Thiago Inácio B Lopes, Hugo Juarez V Pereira, Anita J Marsaioli, Isis M Figueiredo, Josué Carinhanha Caldas Santos
The interactions of tetracycline (TC), oxytetracycline (OTC) and chlortetracycline (CTC) with ovalbumin (OVA), the main allergen protein of egg white, were investigated by molecular spectroscopy and electrophoresis at three pH conditions (1.5, 4.6 and 7.4). Molecular and synchronous fluorescence, UV-vis spectroscopy, electrophoresis and (1)H NMR were used to study the interaction process. Tetracyclines interact with ovalbumin fluorescence by a static quenching mechanism with non-fluorescent complex formation changing the native protein structure...
April 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28423454/uk-neqas-survey-of-allergen-component-testing-across-the-united-kingdom-and-other-european-countries
#18
R Saleem, C Keymer, D Patel, W Egner, A W Rowbottom
The clinical utility of molecular diagnostic approaches in allergy investigation is being recognized increasingly to play a significant role in the management of allergic patients. Determining the sensitization pattern, which is best achieved through the use of component resolved diagnostics (CRD), allows effective risk stratification, appropriate treatment and patient selection for immunotherapy. In order to assess the diagnostic service provisions for in-vitro allergy testing across Europe, a survey was carried out via the total immunoglobulin (Ig)E and specific IgE external quality assurance schemes run by UK National External Quality Assessment Service (NEQAS) Immunology, Immunochemistry and Allergy...
June 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28417623/a-real-life-comparison-between-allergenic-extracts-and-allergenic-molecules
#19
Giorgio Ciprandi, Paola Comite, Francesca Ferrero, Michele Mussap
Serum IgE assay is a mainstay step in the allergy work up. Allergenic extracts and molecular components are available at present. This real life study compared the serum specific IgE levels against allergenic extracts with allergenic molecules in patients allergic to Parietaria, Betulaceae, and mites. This retrospective real life study included 489 subjects with respiratory allergy. Inclusion criteria were 1) documented diagnosis of allergic rhinitis (AR) and/or allergic asthma, and 2) documented allergy to Parietaria judaica (Par j) 2 (216 patients: 112 females, mean age 42 years), or to Betula verrucosa (Bet v) 1 (62 patients: 35 females, mean age 3 years), or Dermatophagoides pteronyssinus (Der p) 1 (211 patients: 107 females, mean age 34 years); and mono-allergy...
February 2017: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/28411163/development-of-a-multi-epitope-peptide-vaccine-inducing-robust-t-cell-responses-against-brucellosis-using-immunoinformatics-based-approaches
#20
Mahdiye Saadi, Ahmad Karkhah, Hamid Reza Nouri
Current investigations have demonstrated that a multi-epitope peptide vaccine targeting multiple antigens could be considered as an ideal approach for prevention and treatment of brucellosis. According to the latest findings, the most effective immunogenic antigens of brucella to induce immune responses are included Omp31, BP26, BLS, DnaK and L7-L12. Therefore, in the present study, an in silico approach was used to design a novel multi-epitope vaccine to elicit a desirable immune response against brucellosis...
April 11, 2017: Infection, Genetics and Evolution
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