keyword
MENU ▼
Read by QxMD icon Read
search

Molecular allergen

keyword
https://www.readbyqxmd.com/read/29350763/molecular-allergen-profiling-in-horses-by-microarray-reveals-fag-e-2-from-buckwheat-as-a-frequent-sensitizer
#1
L Einhorn, G Hofstetter, S Brandt, E K Hainisch, I Fukuda, K Kusano, A Scheynius, I Mittermann, Y Resch-Marat, S Vrtala, R Valenta, E Marti, C Rhyner, R Crameri, R Satoh, R Teshima, A Tanaka, H Sato, H Matsuda, I Pali-Schöll, E Jensen-Jarolim
BACKGROUND: Companion animals are also affected by IgE-mediated allergies, but the eliciting molecules are largely unknown. We aimed at refining an allergen microarray to explore sensitization in horses and compare it to the human IgE reactivity profiles. METHODS: Custom-designed allergen microarray was produced on the basis of the IimmunoCAP ISAC technology containing 131 allergens. Sera from 51 horses derived from Europe or Japan were tested for specific IgE reactivity...
January 19, 2018: Allergy
https://www.readbyqxmd.com/read/29348555/novel-immunoinformatics-approaches-to-design-multi-epitope-subunit-vaccine-for-malaria-by-investigating-anopheles-salivary-protein
#2
Rajan Kumar Pandey, Tarun Kumar Bhatt, Vijay Kumar Prajapati
Malaria fever has been pervasive for quite a while in tropical developing regions causing high morbidity and mortality. The causal organism is a protozoan parasite of genus Plasmodium which spreads to the human host by the bite of hitherto infected female Anopheles mosquito. In the course of biting, a salivary protein of Anopheles helps in blood feeding behavior and having the ability to elicit the host immune response. This study represents a series of immunoinformatics approaches to design multi-epitope subunit vaccine using Anopheles mosquito salivary proteins...
January 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29348379/the-differences-in-serum-quantitative-specific-ige-levels-induced-by-dermatophagoides-pteronyssinus-dermatophagoides-farinae-and-blomia-tropicalis-sensitization-in-intermittent-and-persistent-allergic-asthma
#3
Agus Joko Susanto, Iris Rengganis, Cleopas M Rumende, Kuntjoro Harimurti
BACKGROUND: house dust mites (HDM) are an important inhalant allergen in allergic asthma. However, molecular diagnostic study of specific IgE to HDM allergens has not been done in Indonesia. In addition, the association of quantitative specific IgE measurement with asthma severity has not been investigatedd. This study aimed to investigate the difference of serum quantitative specific IgE levels induced by Dermatophagoides (D.) pteronyssinus, D. farinae and Blomia tropicalis sensitization in intermittent and persistent allergic asthma...
October 2017: Acta Medica Indonesiana
https://www.readbyqxmd.com/read/29344948/glycation-of-ovalbumin-after-high-intensity-ultrasound-pretreatment-effects-on-conformation-igg-ige-binding-ability-and-antioxidant-activity
#4
Wenhua Yang, Zongcai Tu, Hui Wang, Lu Zhang, Qidong Song
BACKGROUND: Ovalbumin (OVA), a protein with excellent nutritional and processing properties, is the major allergen of hen egg white. Previous study showed that high-intensity ultrasound treatment increased the IgG and IgE binding abilities by unfolding the conformational structure of OVA. It may allow modifying the IgG and IgE binding of OVA by combining high-intensity ultrasound with other methods, such as glycation, a promising method for the improvement of protein properties. RESULTS: Glycation with mannose (M) after ultrasound pretreatment at 0-600 W significantly reduced the IgG and IgE binding abilities and dramatically enhanced the antioxidant activity of OVA-M conjugates, with the lowest values of IgG and IgE binding and highest values of antioxidant capacity observed at 600W...
January 18, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29342409/development-and-characterization-of-an-allergoid-of-cat-dander-for-immunotherapy
#5
J P Sola, Y Pedreño, A Cerezo, M Peñalver-Mellado
BACKGROUND: Allergy to cats is a frequent cause of sensitization to indoor allergens and currently there are few alternatives to specific immunotherapy with cat native extracts. The objective is to develop and characterize a new allergoid to increase the tools available for use in clinical practice. METHODS: The allergoid cat dander extract (ACD) was developed from a native cat dander extract (NCD) by modification with glutaraldehyde, and the optimal process control was determined by SDS-PAGE, DOT BLOT and determination of free amine groups...
January 13, 2018: Allergologia et Immunopathologia
https://www.readbyqxmd.com/read/29337221/venom-characterization-of-the-amazonian-scorpion-tityus-metuendus
#6
C V F Batista, J G Martins, R Restano-Cassulini, F I V Coronas, F Z Zamudio, R Procópio, L D Possani
The soluble venom from the scorpion Tityus metuendus was characterized by various methods. In vivo experiments with mice showed that it is lethal. Extended electrophysiological recordings using seven sub-types of human voltage gated sodium channels (hNav1.1 to 1.7) showed that it contains both α- and β-scorpion toxin types. Fingerprint analysis by mass spectrometry identified over 200 distinct molecular mass components. At least 60 sub-fractions were recovered from HPLC separation. Five purified peptides were sequenced by Edman degradation, and their complete primary structures were determined...
January 11, 2018: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/29333852/synthesis-of-mip-nanoparticles-for-%C3%AE-casein-detection-using-spr-as-a-milk-allergen-sensor
#7
Jon Ashley, Yunus Shukor, Roberta D'Aurelio, Linda Trinh, Thomas Lawrence Rodgers, Jeff Temblay, Mike Pleasants, Ibtisam E Tothill
Food recalls due to undeclared allergens or contamination are costly to the food manufacturing industry worldwide. As the industry strives for better manufacturing efficiencies over a diverse range of food products, there is a need for the development of new analytical techniques to improve monitoring the presence of unintended food allergens during the food manufacturing process. In particular, the monitoring of wash samples from cleaning in place systems (CIP), used in the cleaning of food processing equipment, would allow for the effective removal of allergen containing ingredients in between food batches...
January 15, 2018: ACS Sensors
https://www.readbyqxmd.com/read/29331514/employment-of-proteomic-and-immunological-based-methods-for-the-identification-of-catalase-as-novel-allergen-from-banana
#8
Jasna Nikolić, Andrijana Nešić, Skadi Kull, Frauke Schocker, Uta Jappe, Marija Gavrović-Jankulović
Diagnostic reagents based on food allergen extracts often lack sufficient sensitivity. The introduction of well characterized food allergens in molecular allergy diagnosis has been recognized as valid approach to circumvent unstandardized allergen extracts. Banana fruit (Musa acuminata) is a well-established allergen source which besides six characterized allergens, contains unidentified IgE reactive proteins whose clinical relevance remains undefined. By employment of a combinatorial peptide ligand library (CPLL) methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis, a novel allergen from banana fruit was detected in banana as catalase...
January 10, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29331065/component-resolved-diagnostics-to-direct-in-venom-immunotherapy-important-steps-towards-precision-medicine
#9
Simon Blank, Maria Beatrice Bilò, Markus Ollert
Stings of Hymenoptera can induce IgE-mediated systemic and even fatal allergic reactions. Venom-specific immunotherapy (VIT) is the only disease-modifying and curative treatment of venom allergy. However, choosing the correct venom for VIT represents a necessary prerequisite for efficient protection against further anaphylactic sting reactions after VIT. In the past, therapeutic decisions based on the measurement of specific IgE (sIgE) levels to whole venom extracts were not always straightforward, especially when the patient was not able to identify the culprit insect...
January 13, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29323160/glutathione-s-transferase-gst-of-american-cockroach-periplaneta-americana-classes-isoforms-and-allergenicity
#10
Nitat Sookrung, Onrapak Reamtong, Rojana Poolphol, Nitaya Indrawattana, Watee Seesuay, Nawannaporn Saelim, Pongsakorn Tantilipikorn, Chaweewan Bunnag, Wanpen Chaicumpa, Anchalee Tungtrongchitr
Insect glutathione S-transferases (GSTs) play important roles in insecticide/drug resistance and stress response. Medically, GSTs of house dust mites (Dermatophagoides pteronyssinus and Blomia tropicalis) and German cockroach (Blattella germanica) are human allergens. In this study, classes, isoforms and B-cell and allergenic epitopes of GST of American cockroach, Periplaneta americana, the predominant species in the tropics and subtropics were investigated for the first time. Enzymatically active native and recombinant P...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29319884/similar-localization-of-conformational-ige-epitopes-on-the-house-dust-mite-allergens-der-p-5-and-der-p-21-despite-limited-ige-cross-reactivity
#11
M Curin, T Garmatiuk, Y Resch-Marat, K W Chen, G Hofer, K Fauland, W Keller, W Hemmer, S Vrtala, M Focke-Tejkl, R Valenta
BACKGROUND: Due to high IgE recognition frequency and high allergenic activity, Der p 5 and Der p 21 are clinically important house dust mite (HDM) allergens. The objective of this study was to characterise the immunodominant IgE epitopes of Der p 5 and Der p 21 responsible for their high allergenic activity. METHODS: A panel of 12 overlapping peptides spanning the Der p 5 and Der p 21 sequence were synthesized to search for sequential IgE epitopes by direct testing for allergic patients ' IgE reactivity...
January 10, 2018: Allergy
https://www.readbyqxmd.com/read/29318625/consensus-document-on-dog-and-cat-allergy
#12
REVIEW
Ignacio Davila, Javier Domínguez-Ortega, Ana Mª Navarro Pulido, Alicia Alonso, Dario Antolin-Amerigo, Eloina González-Mancebo, Cristina Martin Garcia, Beatriz Nuñez-Acevedo, Nieves Prior, Marta Reche, Ana Rosado, Javier Ruiz-Hornillos, María Cesárea Sánchez, Miguel Torrecillas
The prevalence of sensitisation to dogs and cats varies by country, exposure time and predisposition to atopy. It is estimated that, 26% of European adults coming to the clinic for suspected allergy to inhalant allergens are sensitised to cats and 27% to dogs. This document is intended to be a useful tool for clinicians involved in the management of people with dog or cat allergy. It was prepared from a consensus process based on the RAND/UCLA method. Following a literature review, it proposes various recommendations concerning the diagnosis and treatment of these patients, grounded in evidence and clinical experience...
January 10, 2018: Allergy
https://www.readbyqxmd.com/read/29290503/-evaluation-of-sensitization-to-der-p-1-and-der-p-2-in-a-pediatric-population-of-the-north-of-portugal
#13
Sara Peixoto, Joana Soares, Tânia Monteiro, Marisa Carvalho, Marinela Santos, Carla Simões, Márcia Quaresma
INTRODUCTION: In Portugal, data on the role of Der p 1 and Der p 2 in patients with house dust mite (HDM) allergy are scarce. Allergen-specific immunotherapy (sIT) is the only treatment that improves symptoms, reduces the need for pharmacological therapy and modifies the natural history of the disease. With this study, the authors aim to understand the local epidemiology and to clarify if the molecular assay of major allergens is advantageous in deciding and/or modifying the decision to initiate sIT in children with clinical indication which are sensitized to Dermatophagoides pteronyssinus...
December 28, 2017: Anales de Pediatría: Publicación Oficial de la Asociación Española de Pediatría (A.E.P.)
https://www.readbyqxmd.com/read/29285938/novel-kunitz-like-peptides-discovered-in-the-zoanthid-palythoa-caribaeorum-through-transcriptome-sequencing
#14
Qiwen Liao, Shengnan Li, Shirley Weng In Siu, Binrui Yang, Chen Huang, Judy Yuet-Wa Chan, Jean-Étienne Morlighem, Clarence Tsun Ting Wong, Gandhi Radis-Baptista, Simon Ming-Yuen Lee
Palythoa caribaeorum (class Anthozoa) is a zoanthid that together jellyfishes, hydra, and sea anemones, which are venomous and predatory, belongs to the Phyllum Cnidaria. The distinguished feature in these marine animals is the cnidocytes in the body tissues, responsible for toxin production and injection that are used majorly for prey capture and defense. With exception for other anthozoans, the toxin cocktails of zoanthids have been scarcely studied and are poorly known. Here, based on the analysis of P. caribaeorum transcriptome, numerous predicted venom-featured polypeptides were identified, including allergens, neurotoxins, membrane-active and Kunitz-like peptides (PcKuz)...
December 29, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29280148/bioactive-hydrolysates-from-casein-generation-identification-and-in-silico-toxicity-and-allergenicity-prediction-of-peptides
#15
Maolin Tu, Hanxiong Liu, Hui Chen, Fengjiao Fan, Pujie Shi, Xianbing Xu, Weihong Lu, Ming Du
BACKGROUND: Bioactive casein peptides have attracted considerable attention for their applications in industry. However, there is little clarity regarding mass spectrometric profiles for peptides in enzymatic hydrolysates of casein produced under varying conditions. In this study, the compositions of the peptides from casein hydrolysates were compared for different E/S and hydrolysis times. The toxicity, allergenicity and bioactivity of the identified peptides were assessed in silico...
December 27, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29277308/nrf2-as-regulator-of-innate-immunity-a-molecular-swiss-army-knife
#16
REVIEW
Maurizio Battino, Francesca Giampieri, Francesca Pistollato, Antoni Sureda, Marcos Roberto de Oliveira, Valeria Pittalà, Nikolay Mehterov, Victoria Sarafian, Francesca Fallarino, Seyed Fazel Nabavi, Atanas Georgiev Atanasov, Seyed Mohammad Nabavi
Organisms are constantly exposed to a broad range of pathological and stress-inducing agents, allergens and environmental chemicals that can induce infections, toxicity or other undesirable reactions. Our immune system has evolved over time in order to efficiently respond to these exogenous insults and maintain homeostasis. In particular, the innate immune system acts as primary barrier to prevent the entrance of invasive agents or allergens. This system is comprised of a diversity of cell types that are rapidly activated by recognition of common structures present in many potential pathogens known as pathogen-associated molecular patterns (PAMPs)...
December 22, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/29249755/-molecular-mechanisms-for-cutaneously-sensitized-food-allergy-experimental-evidences-for-dual-allergen-exposure-hypothesis
#17
Tomohiro Yoshimoto
No abstract text is available yet for this article.
2017: Arerugī, [Allergy]
https://www.readbyqxmd.com/read/29248116/molecular-diversity-and-allergenic-profiles-of-alternaria-spp-from-desert-environments-in-arizona
#18
Francesca Rotondo, Soon Gyu Hong, Tobin Peever, Barry M Pryor
This study examined the genetic diversity of small-spored Alternaria species in the southwest desert of the USA by sampling 552 isolates from different habitats (soil and plant debris) in different locations (urban and an undisturbed desert). To estimate the genetic diversity, Amplified Fragment Length Polymorphism (AFLP) fingerprinting analysis was performed for all isolates. Strains representative of the sampled genotypic diversity (n = 125) were further characterized according their sporulation pattern and the capability to produce allergens...
January 2018: Fungal Biology
https://www.readbyqxmd.com/read/29247241/beneficial-effects-of-melittin-on-ovalbumin-induced-atopic-dermatitis-in-mouse
#19
Woon-Hae Kim, Hyun-Jin An, Jung-Yeon Kim, Mi-Gyeong Gwon, Hyemin Gu, Minji Jeon, Woo Jung Sung, Sang Mi Han, Sok Cheon Pak, Min-Kyung Kim, Kwan-Kyu Park
Atopic dermatitis (AD) is an inflammatory skin disease characterized by intense pruritus and relapsable eczematous lesions. The hallmarks of AD are defects in the epidermal barrier and immunoglobulin E (IgE)-mediated sensitization to several environmental allergens, as well as an immune disorder mediated by an imbalance toward T-helper-2 response. Melittin, a major component of bee venom, has been studied in various inflammatory diseases. However, the beneficial effects of melittin on mouse with AD-like symptoms have not been explored...
December 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29228796/component-resolved-microarray-analysis-of-ige-sensitization-profiles-to-felis-catus-major-allergen-molecules-in-russian-cat-allergic-patients
#20
Anna Sergeevna Dolgova, Anna Evgenevna Sudina, Anna Sergeevna Cherkashina, Olga Alekseevna Stukolova
We aimed to determine the profile of IgE reactivity to three major cat allergens, Fel d 1, Fel d 2 and Fel d 4, in cat-allergic patients in the Moscow region in Russia. sIgE levels to recombinant proteins expressed in Escherichia coli (Fel d 1 and Fel d 4) and to Fel d 2 protein purified from cat serum were measured using a microarray method developed in our laboratory. Sera from 174 anonymous subjects with a positive reaction (≥0.35 IU/mL) to cat dander extract (e1, ImmunoCAP) and 56 negative controls were used for IgE testing...
December 11, 2017: Scandinavian Journal of Clinical and Laboratory Investigation
keyword
keyword
100378
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"