Calum Forteath, Ify Mordi, Raid Nisr, Erika J Gutierrez-Lara, Noor Alqurashi, Iain R Phair, Amy R Cameron, Craig Beall, Ibrahim Bahr, Mohapradeep Mohan, Aaron K F Wong, Adel Dihoum, Anwar Mohammad, Colin N A Palmer, Douglas Lamont, Kei Sakamoto, Benoit Viollet, Marc Foretz, Chim C Lang, Graham Rena
OBJECTIVE: Unexplained changes in regulation of branched chain amino acids (BCAA) during diabetes therapy with metformin have been known for years. Here we have investigated mechanisms underlying this effect. METHODS: We used cellular approaches, including single gene/protein measurements, as well as systems-level proteomics. Findings were then cross-validated with electronic health records and other data from human material. RESULTS: In cell studies, we observed diminished uptake/incorporation of amino acids following metformin treatment of liver cells and cardiac myocytes...
August 2023: Molecular Metabolism