keyword
MENU ▼
Read by QxMD icon Read
search

succinate

keyword
https://www.readbyqxmd.com/read/28926680/formic-acid-as-a-secondary-substrate-for-succinic-acid-production-by-metabolically-engineered-mannheimia-succiniciproducens
#1
Jung Ho Ahn, Junho Bang, Won Jun Kim, Sang Yup Lee
There has been much effort exerted to reduce one carbon (C1) gas emission to address climate change. As one promising way to more conveniently utilize C1 gas, several technologies have been developed to convert C1 gas into useful chemicals such as formic acid (FA). In this study, systems metabolic engineering was utilized to engineer Mannheimia succiniciproducens to efficiently utilize FA. (13) C isotope analysis of M. succiniciproducens showed that FA could be utilized through formate dehydrogenase (FDH) reaction and/or the reverse reaction of pyruvate formate lyase (PFL)...
September 19, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28924001/diagnosis-of-endocrine-disease-sdhx-mutations-beyond-pheochromocytomas-and-paragangliomas
#2
Massimo Mannelli, Letizia Canu, Tonino Ercolino, Elena Rapizzi, Serena Martinelli, Gabriele Parenti, Giuseppina De Filpo, Gabriella Nesi
Mutations in one of the five genes encoding the succinate dehydrogenase (SDHx) or mitochondrial complex II cause the corresponding family syndromes characterized by the occurrence of pheochromocytomas (PHEO) and paragangliomas (PGL). Recently, other solid growths, such as gastrointestinal stromal tumors (GISTs), renal cell carcinomas (RCCs) and pituitary adenomas (PAs) have been associated with these syndromes. In the absence of prospective studies assessing their frequency, at present, their occurrence seems too infrequent to suggest systematic screening for SDHx mutation carriers...
September 18, 2017: European Journal of Endocrinology
https://www.readbyqxmd.com/read/28920748/investigation-of-the-transport-and-absorption-of-angelica-sinensis-polysaccharide-through-gastrointestinal-tract-both-in-vitro-and-in-vivo
#3
Kaiping Wang, Fang Cheng, Xianglin Pan, Tao Zhou, Xiqiu Liu, Ziming Zheng, Li Luo, Yu Zhang
To investigate the absorption and delivery of ASP in gastrointestinal (GI) tract, cASP was successfully synthesized by chemically modifying with succinic anhydride and then conjugating with a near infrared fluorescent dye Cy5.5. Then, the capacity of oral absorption of cASP was evaluated. The results demonstrated that cASP had low toxicity and no disruption on the integrity of cell membrane. The endocytosis of cASP into the epithelial cells was time- and energy-dependent, which was mediated by macropinocytosis pathway and clathrin- and caveolae (or lipid raft)-related routes...
November 2017: Drug Delivery
https://www.readbyqxmd.com/read/28919252/mutation-in-the-peroxin-coding-gene-pex22-contributing-to-high-malate-production-in-saccharomyces-cerevisiae
#4
Hiroaki Negoro, Mitsuru Sakamoto, Atsushi Kotaka, Kengo Matsumura, Yoji Hata
Saccharomyces cerevisiae produces organic acids such as succinate, acetate, and malate during alcoholic fermentation. Since malate contributes to the pleasant taste of sake (a Japanese alcoholic beverage), various methods for breeding high-malate-producing yeast strains have been developed. Here, a high-malate-producing yeast strain F-701H was isolated. This mutant was sensitive to dimethyl succinate (DMS) and harbored a nonsense mutation in the peroxin gene PEX22, which was identified as the cause of high malate production by comparative genome analysis...
September 14, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28918749/effect-of-low-temperature-on-globin-expression-respiratory-metabolic-enzyme-activities-and-gill-structure-of-litopenaeus-vannamei
#5
Meng Wu, Nan Chen, Chun-Xiao Huang, Yan He, Yong-Zhen Zhao, Xiao-Han Chen, Xiu-Li Chen, Huan-Ling Wang
Low temperature frequently influences growth, development, and even survival of aquatic animals. In the present study, physiological and molecular responses to low temperature in Litopenaeus vannamei were investigated. The cDNA sequences of two oxygen-carrying proteins, cytoglobin (Cygb) and neuroglobin (Ngb), were isolated. Protein structure analysis revealed that both proteins share a globin superfamily domain. Real-time PCR analysis indicated that Cygb and Ngb mRNA levels gradually increased during decrease in temperatures from 25 to 15°C and then decreased at 10°C in muscle, brain, stomach, and heart, except for a continuing increase in gills, whereas they showed a different expression trend in the hepatopancreas...
July 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28918353/antitumor-platinum-iv-derivatives-of-carboplatin-and-the-histone-deacetylase-inhibitor-4-phenylbutyric-acid
#6
Awatif Rashed Z Almotairy, Valentina Gandin, Liam Morrison, Cristina Marzano, Diego Montagner, Andrea Erxleben
Five new platinum(IV) derivatives of carboplatin each incorporating the histone deacetylase inhibitor 4-phenylbutyrate in axial position were synthesized and characterized by (1)H and (195)Pt NMR spectroscopy, electrospray ionization mass spectrometry and elemental analysis, namely cis,trans-[Pt(CBDCA)(NH3)2(PBA)(OH)] (1), cis,trans-[Pt(CBDCA)(NH3)2(PBA)2] (2), cis,trans-[Pt(CBDCA)(NH3)2(PBA)(bz)] (3), cis,trans-[Pt(CBDCA)(NH3)2(PBA)(suc)] (4) and cis,trans-[Pt(CBDCA)(NH3)2)(PBA)(ac)] (5) (PBA=4-phenylbutyrate, CBDCA=1,1-cyclobutane dicarboxylate, bz=benzoate, suc=succinate and ac=acetate)...
September 10, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28917891/pickering-emulsifiers-based-on-hydrophobically-modified-small-granular-starches-part-i-manufacturing-and-physico-chemical-characterization
#7
A Marefati, B Wiege, N U Haase, M Matos, M Rayner
Small granular starches from rice, quinoa and amaranth were hydrophobized by esterification with octenyl succinic anhydride (OSA) in an aqueous alkaline slurry to obtain series of modified starches at defined intervals (i.e. 0.6, 1.2, 1.8, 2.4, 3.0%). The physical and the physico-chemical properties of the starch particles were characterized by proximate analysis including protein level, amylose level and dry matter. The shape and size of the starch granules were characterized by scanning electron microscopy and light scattering...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917877/effects-of-hydrothermal-alkali-and-freezing-thawing-pre-treatments-on-modification-of-corn-starch-with-octenyl-succinic-anhydride
#8
Shujun Wang, Tiangui Li, Shuo Wang, Les Copeland
Pre-treatments by freezing/thawing (F/T) and hydrothermal-alkali (HA) were used in the modification of starch with octenyl succinic anhydride (OSA). In comparison with OSA-modified starch prepared without pre-treatment (degree of substitution, DS=0.0172; reaction efficiency, RE=73.9%), HA pre-treatment increased the DS of OSA-starch and RE of subsequent OSA modification (0.0185 and 79.7%, respectively). In contrast, F/T pre-treatment gave the opposite results (0.0152 and 65.5%, respectively). OSA modification markedly increased the viscosities but decreased the gelatinization parameters of native starch...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28916886/construction-of-fibrous-bed-bioreactor-for-enhanced-succinic-acid-production-using-wastewater-of-dextran-fermentation
#9
Peng-Cheng Chen, Xin-Hua Zha, Pu Zheng
A new applicability of wastewater of dextran fermentation (WWDF) for biological production of succinic acid with A. succinogenes CCTCC M2012036 was reported in this work for the first time. Notably, K2CO3 was used instead of MgCO3 in the pH regulating process for operational feasibility and a cell immobilization methodology by attaching cells on cotton fibrous matrix was adopted for cell recycle. The initial sugar concentration as well as matrix usage was optimized by investigating the cell growth, succinic acid concentration and yield...
September 15, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28916561/succinate-transport-is-not-essential-for-symbiotic-nitrogen-fixation-by-sinorhizobium-meliloti-nor-rhizobium-leguminosarum
#10
Michael J Mitsch, George C diCenzo, Alison Cowie, Turlough M Finan
Symbiotic nitrogen fixation (SNF) is an energetically expensive process performed by bacteria during endosymbiotic relationships with plants. The bacteria require the plant to provide a carbon source for generation of reductant to power SNF. While C4-dicarboxylates (succinate, fumarate, malate) appear to be the primary, if not sole, carbon source provided to the bacteria, the contribution of each C4-dicarboxylate is not known. We address this issue using a genetic and systems-level analyses. Expression of a malate specific transporter (MaeP) in Sinorhizobium meliloti Rm1021 dct mutants unable to transport C4-dicarboxylates resulted in malate import rates up to 30% that of wild type...
September 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28916461/metabolic-engineering-of-escherichia-coli-for-the-synthesis-of-the-quadripolymer-poly-glycolate-co-lactate-co-3-hydroxybutyrate-co-4-hydroxybutyrate-from-glucose
#11
Zheng-Jun Li, Kangjian Qiao, Xue-Mei Che, Gregory Stephanopoulos
Escherichia coli was metabolically engineered to effectively produce a series of biopolymers consisted of four types of monomers including glycolate, lactate, 3-hydroxybutyrate and 4-hydroxybutyrate from glucose as the carbon source. The biosynthetic route of novel quadripolymers was achieved by the overexpression of a range of homologous and heterologous enzymes including isocitrate lyase, isocitrate dehydrogenase kinase/phosphatase, glyoxylate/hydroxypyruvate reductase, propionyl-CoA transferase, β-ketothiolase, acetoacetyl-CoA reductase, succinate semialdehyde dehydrogenase, 4-hydroxybutyrate dehydrogenase, CoA transferase and PHA synthase...
September 12, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28916229/japanese-leigh-syndrome-case-treated-with-epi-743
#12
Takeshi Kouga, Mariko Takagi, Akihiko Miyauchi, Hiroko Shimbo, Mizue Iai, Sumimasa Yamashita, Kei Murayama, Matthew B Klein, Guy Miller, Tomohide Goto, Hitoshi Osaka
BACKGROUND: Leigh syndrome is a mitochondrial disease caused by respiratory chain deficiency, and there are no proven effective therapies. EPI-743 is a potent cellular oxidative stress protectant and results of clinical trials for mitochondrial diseases are accumulating. CASE: At 5months, a girl presented with the scarce eye movement and diminished muscle tone. She was diagnosed with Leigh encephalopathy from blood and cerebrospinal fluid lactate elevation and MRI findings...
September 12, 2017: Brain & Development
https://www.readbyqxmd.com/read/28914292/ph-and-redox-dual-sensitive-polysaccharide-nanoparticles-for-the-efficient-delivery-of-doxorubicin
#13
Shengcai Yang, Zhaohui Tang, Dawei Zhang, Mingxiao Deng, Xuesi Chen
A pH and redox dual-sensitive biodegradable polysaccharide, succinic acid-decorated dextran-g-phenylalanine ethyl ester-g-cysteine ethyl ester (Dex-SA-l-Phe-l-Cys), was synthesized to load doxorubicin hydrochloride (DOX·HCl). The DOX-loaded nanoparticles, which were prepared in aqueous solution and free of organic solvent, could spontaneously self-assemble into uniform sizes. When loading DOX·HCl, mercapto Dex-SA-l-Phe-l-Cys was oxidized into a crosslinked disulfide linkage to form pH and redox dual-sensitive nanoparticles (DOX-S-NPs)...
September 15, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28914132/reverse-electron-transfer-results-in-a-loss-of-flavin-from-mitochondrial-complex-i-potential-mechanism-for-brain-ischemia-reperfusion-injury
#14
Anna Stepanova, Anja Kahl, Csaba Konrad, Vadim Ten, Anatoly S Starkov, Alexander Galkin
Ischemic stroke is one of the most prevalent sources of disability in the world. The major brain tissue damage takes place upon the reperfusion of ischemic tissue. Energy failure due to alterations in mitochondrial metabolism and elevated production of reactive oxygen species (ROS) is one of the main causes of brain ischemia-reperfusion (IR) damage. Ischemia resulted in the accumulation of succinate in tissues, which favors the process of reverse electron transfer (RET) when a fraction of electrons derived from succinate is directed to mitochondrial complex I for the reduction of matrix NAD(+)...
January 1, 2017: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/28912768/linseed-oil-supplementation-of-lambs-diet-in-early-life-leads-to-persistent-changes-in-rumen-microbiome-structure
#15
Tamsin Lyons, Tommy Boland, Sean Storey, Evelyn Doyle
Diet has been shown to have a significant impact on microbial community composition in the rumen and could potentially be used to manipulate rumen microbiome structure to achieve specific outcomes. There is some evidence that a window may exist in early life, while the microbiome is being established, where manipulation through diet could lead to long-lasting results. The aim of this study was to test the hypothesis that dietary supplementation in early life will have an effect on rumen microbial composition that will persist even once supplementation is ceased...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28912270/the-periplasmic-transaminase-ptaa-of-pseudomonas-fluorescens-converts-the-glutamic-acid-residue-at-the-pyoverdine-fluorophore-to-%C3%AE-ketoglutaric-acid
#16
Michael T Ringel, Gerald Draeger, Thomas Brueser
The periplasmic conversion of ferribactin to pyoverdine is essential for siderophore biogenesis in fluorescent pseudomonads, such as pathogenic Pseudomonas aeruginosa or plant growth promoting P. fluorescens The non-ribosomal peptide ferribactin undergoes cyclizations and oxidations that result in the fluorophore, and a strictly conserved fluorophore-bound glutamic acid residue is converted to a range of variants, including succinamide, succinic acid, and α-ketoglutaric acid residues. We recently discovered that the pyridoxal phosphate (PLP)-containing enzyme PvdN is responsible for the generation of the succinamide, which can be hydrolyzed to succinic acid...
September 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28910984/genomic-and-transcriptomic-analysis-of-saccharomyces-cerevisiae-isolates-with-focus-in-succinic-acid-production
#17
Ricardo Franco-Duarte, Daniela Bessa, Filipa Gonçalves, Rosa Martins, António César Silva-Ferreira, Dorit Schuller, Paula Sampaio, Célia Pais
Succinic acid is a platform chemical that plays an important role as precursor for the synthesis of many valuable bio-based chemicals. Its microbial production from renewable resources has seen great developments, specially exploring the use of yeasts to overcome the limitations of using bacteria. The objective of the present work was to screen for succinate-producing isolates, using a yeast collection with different origins and characteristics. Four strains were chosen, two as promising succinic acid producers, in comparison with two low producers...
September 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28910980/neuroprotective-effect-of-deferoxamine-on-n-methyl-d-aspartate-induced-excitotoxicity-in-rgc-5-cells
#18
Ying Tian, Ye He, Weitao Song, Endong Zhang, Xiaobo Xia
Many N-methyl-d-aspartate (NMDA) receptor antagonists have been used to treat neurodegenerative diseases induced by glutamate excitotoxicity in clinics. However, the universality of the glutamic acid neurotransmitter system makes the glutamic acid receptor blockers inefficient and unsafe. Thus, regulating the downstream signaling pathway in the excitotoxicity of glutamic acid may be a more effective and safer way to antagonize the glutamic acid receptor. In this study, we investigated the effect of deferoxamine (DFO), an iron chelator, on the NMDA-induced excitotoxicity...
September 1, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28904860/regulation-of-neisseria-meningitidis-cytochrome-bc1-components-by-nrrf-a-fur-controlled-small-noncoding-rna
#19
Yvonne Pannekoek, Robert Huis In 't Veld, Kim Schipper, Sandra Bovenkerk, Gertjan Kramer, Dave Speijer, Arie van der Ende
NrrF is a small regulatory RNA of the human pathogen Neisseria meningitidis. NrrF was previously shown to repress succinate dehydrogenase (sdhCDAB) under control of the ferric uptake regulator (Fur). Here, we provide evidence that cytochrome bc1 , encoded by the polycistronic mRNA petABC, is a NrrF target as well. We demonstrated differential expression of cytochrome bc1 comparing wild-type meningococci and meningococci expressing NrrF when sufficient iron is available. Using a gfp-reporter system monitoring translational control and target recognition of sRNA in Escherichia coli, we show that interaction between NrrF and the 5' untranslated region of the petABC mRNA results in its repression...
September 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28903185/mixed-micelles-for-encapsulation-of-doxorubicin-with-enhanced-in-vitro-cytotoxicity-on-breast-and-ovarian-cancer-cell-lines-versus-doxil-%C3%A2
#20
Maximiliano Cagel, Ezequiel Bernabeu, Lorena Gonzalez, Eduardo Lagomarsino, Marcela Zubillaga, Marcela A Moretton, Diego A Chiappetta
Doxorubicin (DOX) is used as a "first-line" antineoplastic drug in ovarian and metastatic breast cancer. However, serious side effects, such as cardiotoxicity have been reported after DOX intravenous administration. Hence, we investigated different micelle-former biomaterials, as Soluplus(®), Pluronic F127, Tetronic T1107 and d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) to develop a potential mixed micellar nanocarrier for DOX delivery. Since DOX hydrochloride is a poor candidate to be encapsulated inside the hydrophobic core of the mixed micelles, we assayed a hydrophobic complex between DOX and sodium deoxycholate (NaDC) as an excellent candidate to be encapsulated within polymeric micelles...
September 9, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
keyword
keyword
100287
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"