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Mohamed A Farag, Amira R Khattab, Anja Ehrlich, Matthias Kropf, Andreas G Heiss, Ludger A Wessjohann
Lens culinaris and several Lupinus species are two legumes regarded as potential protein resources aside from their richness in phytochemicals. Characterization of their metabolite composition is warranted as a sustainable functional food. This study presents a discriminatory-holistic approach for metabolites profiling in accessions of four lentil cultivars and four lupinus species via gas chromatography-mass spectrometry. A total of 107 metabolites were identified encompassing organic and amino acids, sugars and sterols along with anti-nutrients viz...
March 21, 2018: Journal of Agricultural and Food Chemistry
Diego de Souza Gonçalves, Marina da Silva Ferreira, Susie Coutinho Liedke, Kamilla Xavier Gomes, Gabriel Afonso de Oliveira, Pedro Ernesto Lopes Leão, Gabriele Vargas Cesar, Sergio H Seabra, Juliana Reis Cortines, Arturo Casadevall, Leonardo Nimrichter, Gilberto Barbosa Domont, Magno Rodrigues Junqueira, Jose Mauro Peralta, Allan J Guimaraes
Acanthamoeba castellanii (Ac) are ubiquitously distributed in nature, and by contaminating medical devices such as heart valves and contact lenses, they cause a broad range of clinical presentations to humans. Although several molecules have been described to play a role in Ac pathogenesis, including parasite host-tissue invasion and escaping of host-defense, little information is available on their mechanisms of secretion. Herein, we describe the molecular components secreted by Ac, under different protein availability conditions to simulate host niches...
March 21, 2018: Virulence
Fazeela Mahaboob Begum S M, Kalai Chitra, Benin Joseph, Raji Sundararajan, Hemalatha S
BACKGROUND: Lung adenocarcinoma is the most common subtype of Non small cell lung cancer in which the PI3K/Akt cascade is frequently deregulated. The ubiquitous expression of the PI3K and the frequent inactivation of PTEN accounts for the prolonged survival, evasion of apoptosis and metastasis in cancer. This has led to the development of PI3K inhibitors in the treatment of cancer. Synthetic PI3K inhibitors undergoing clinical and preclinical studies are toxic in animals. Hence, there is a critical need to identify PI3K inhibitor(s) of natural origin...
March 20, 2018: BMC Complementary and Alternative Medicine
Dai Long Vu, Karolína Ranglová, Jan Hájek, Pavel Hrouzek
Quantification of selenated amino-acids currently relies on methods employing inductively coupled plasma mass spectrometry (ICP-MS). Although very accurate, these methods do not allow the simultaneous determination of standard amino-acids, hampering the comparison of the content of selenated versus non-selenated species such as methionine (Met) and selenomethionine (SeMet). This paper reports two approaches for the simultaneous quantification of Met and SeMet. In the first approach, standard enzymatic hydrolysis employing Protease XIV was applied for the preparation of samples...
March 9, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Alfonso Méndez-Bravo, Elvis Marian Cortazar-Murillo, Edgar Guevara-Avendaño, Oscar Ceballos-Luna, Benjamín Rodríguez-Haas, Ana L Kiel-Martínez, Orlando Hernández-Cristóbal, José A Guerrero-Analco, Frédérique Reverchon
Rhizobacteria associated with crops constitute an important source of potentially beneficial microorganisms with plant growth promoting activity or antagonistic effects against phytopathogens. In this study, we evaluated the plant growth promoting activity of 11 bacterial isolates that were obtained from the rhizosphere of healthy avocado trees and from that of avocado trees having survived root rot infestations. Seven bacterial isolates, belonging to the genera Bacillus, Pseudomonas and Arthrobacter, promoted in vitro growth of Arabidopsis thaliana...
2018: PloS One
Parva Nasimi, Mohammad Reza Tabandeh, Sayad Roohi
Here, we studied the protective effects of Satureja Khuzestanica essential oil (SKEO) as a potent anti-oxidant, against damage caused by chemotherapy with busulfan in testis and epididymal sperm of adult mice. The NMRI adult mice were assigned: G1: control, G2: was treated with busulfan (4 days, 3.2 mg/kg), G3: was treated with busulfan (4 days, 3.2 mg/kg) and SKEO (28 days, 225 mg/kg) at the same time, and G4: was pre-treated with SKEO (7 days, 225 mg/kg) and subsequently co-treated with busulfan (4 days, 3...
March 20, 2018: Systems Biology in Reproductive Medicine
Ram Chandra, Vineet Kumar, Sonam Tripathi
The extracted sugarcane molasses-melanoidins showed the presence of Mn (8.20), Cr (2.97), Zn (16.61), Cu (2.55), Fe (373.95), Pb (2.59), and Ni (4.18 mg L-1 ) along with mixture of other organic compounds which have endocrine-disrupting chemicals (EDCs) properties. A consortium of aerobic bacteria comprising Klebsiella pneumoniae (KU321273), Salmonella enteric (KU726954), Enterobacter aerogenes (KU726955), and Enterobacter cloacae (KU726957) showed the optimum decolourisation of molasses-melanoidins up to 81% through co-metabolism in the presence of glucose (1...
April 2018: 3 Biotech
Zahra Heidari, Ali Salehzadeh, Seyed Ataollah Sadat Shandiz, Sara Tajdoost
This study highlights the anti-oxidant and anti-cancer activities of bio-functionalized Thymus vulgaris silver nanoparticles (TVAgNPs) and bioactive compounds were compared using the human breast cancer T47D cell line. The aqueous ethanolic extract of T. vulgaris evaluated for chemical composition using the gas chromatography-mass spectrometer (GC-MS) analysis. The prepared TVAgNPs were determined by means of UV-Vis spectroscopy, FTIR spectroscopy, zeta potential, scanning electron microscopy, transmission electron microscopy, and energy-dispersed spectroscopy analysis...
March 2018: 3 Biotech
R Arunadevi, R Susila, S Murugammal, S Divya
BACKGROUND: Increasing popularity of Mathan Tailam (mattan tailam, pachai ennai) for the treatment of diabetic foot ulcer necessitated standardization and quality control of this medicated oil both in large scale production and quality check in marketed drug. OBJECTIVE: Present study aims to develop standard operating procedure for the preparation of Mathan Tailam as per Siddha Formulary of India and its standardization using suitable analytical techniques. MATERIALS AND METHODS: Mathan Tailam was prepared as per Siddha Formulary of India...
March 16, 2018: Journal of Ayurveda and Integrative Medicine
Buchun Si, Jiaming Li, Zhangbing Zhu, Mengmeng Shen, Jianwen Lu, Na Duan, Yuanhui Zhang, Qiang Liao, Yun Huang, Zhidan Liu
One critical challenge of hydrothermal liquefaction (HTL) is its complex aqueous product, which has a high concentration of organic pollutants (up to 100gCOD/L) and diverse fermentation inhibitors, such as furfural, phenolics and N-heterocyclic compounds. Here we report continuous anaerobic digestion of HTL wastewater via an up-flow anaerobic sludge bed reactor (UASB) and packed bed reactor (PBR). Specifically, we investigated the transformation of fermentation inhibitors and microbial response. GC-MS identified the complete degradation of furfural and 5-hydroxymethylfurfural (5-HMF), and partial degradation (54...
March 1, 2018: Science of the Total Environment
Mohammed Mansour Quradha, Rasool Khan, Mujeeb-Ur- Rehman, Abdulrahman Abohajeb
Chemical composition, antioxidant, anticancer, and antimacrobial activities of essential oil obtained from leaves of Rumex nervosus has been evaluated here for the first time. GC/MS analysis reveals the presence of Palmitoleic Acid (28.35%) and Palmitic acid, (25. 37%) as their methyl ester as major components. The essential oil showed significant DPPH radical scavenging activity (94.907 ± 0.1089% and 94.003 ± 0.0749%) at concentration (100 and 80) μg/mL respectively. The oil showed promising activity against staph aureus, while showed weak activity against (Hela and 3T3) cell lines...
March 19, 2018: Natural Product Research
Wilber Escorcia, Dana L Ruter, James Nhan, Sean P Curran
Caenorhabditis elegans is an exceptional model organism in which to study lipid metabolism and energy homeostasis. Many of its lipid genes are conserved in humans and are associated with metabolic syndrome or other diseases. Examination of lipid accumulation in this organism can be carried out by fixative dyes or label-free methods. Fixative stains like Nile red and oil red O are inexpensive, reliable ways to quantitatively measure lipid levels and to qualitatively observe lipid distribution across tissues, respectively...
March 5, 2018: Journal of Visualized Experiments: JoVE
Alan J Bergmann, Gary L Points, Richard P Scott, Glenn Wilson, Kim A Anderson
With hundreds of thousands of chemicals in the environment, effective monitoring requires high-throughput analytical techniques. This paper presents a quantitative screening method for 1550 chemicals based on statistical modeling of responses with identification and integration performed using deconvolution reporting software. The method was evaluated with representative environmental samples. We tested biological extracts, low-density polyethylene, and silicone passive sampling devices spiked with known concentrations of 196 representative chemicals...
March 19, 2018: Analytical and Bioanalytical Chemistry
Ting Deng, Lei Wu, Jun-Min Gao, Bin Zhou, Ya-Li Zhang, Wen-Nan Wu, Zhuo-Heng Tang, Wen-Chao Jiang, Wei-Lin Huang
The occurrence and health risks of organotins (OTs) in the waterworks and source water in the Three Gorges Reservoir Region (TGRR), China were assessed in this study. Water samples were collected at four waterworks (A, B, C, and D) in March and July 2012 to analyze butyltins (BTs) and phenyltins (PTs) using a gas chromatography-mass spectrometry (GC-MS) system. Our results showed that both the waterworks and their nearby water sources were polluted by OTs, with PTs being the most dominant species. Monobutyltin (MBT), monophenyltin (MPT), diphenyltin (DPT), and triphenyltin (TPT) were detected in most of the analyzed water samples...
March 18, 2018: Environmental Science and Pollution Research International
M Rath, T R Mitchell, S E Gold
Volatile organic compounds (VOCs) produced by Plant Growth Promoting Rhizobacteria have recently been investigated due to their role in plant growth promotion and defense. Whereas some bacterial VOCs like 3-hydroxy-2-butanone (acetoin) and 2,3-butanediol produced by strains of Bacillus subtilis and Bacillus amyloliquefaciens promote plant growth, others like hydrogen cyanide and 3-phenylpropionic acid are phytotoxic, inhibiting plant growth. Bacillus mojavensis, a close relative of B. subtilis, is an endophytic bacterium of maize that has been shown to have antagonistic activity against the mycotoxigenic phytopathogen Fusarium verticillioides and growth promotion activity on maize seedlings...
March 2018: Microbiological Research
Wandayi Emmanuel Amlabu, Ishaya Haruna Nock, Naveen Kumar Kaushik, Dinesh Mohanakrishnan, Jyoti Tiwary, Patrick Ahmadu Audu, Musbahu S Abubakar, Dinkar Sahal
The plant kingdom continues to hold great promise for the eradication of Malaria infection following the challenges of insecticide resistance by the vector mosquito, drug resistance by the parasite, and the development of a vaccine still being a mirage. Acalypha wilkesiana Muller Argoviensis, 1866 (family: Euphorbiaceae) leaves have the ethnopharmacological reputation for use as a remedy against dermal microbial infections in Nigeria. Here, we have studied the antiplasmodial potential of the extract of the leaves of this ornamental plant...
March 17, 2018: Parasitology Research
Masoud Ghader, Nader Shokoufi, Ali Es-Haghi, Kazem Kargosha
Vaccine production is a biological process in which variation in time and output is inevitable. Thus, the application of Process Analytical Technologies (PAT) will be important in this regard. Headspace solid - phase microextraction (HS-SPME) coupled with GC-MS can be used as a PAT for process monitoring. This method is suitable to chemical profiling of volatile organic compounds (VOCs) emitted from microorganisms. Tetanus is a lethal disease caused by Clostridium tetani (C. tetani) bacterium and vaccination is an ultimate way to prevent this disease...
February 6, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Ram Naresh Bharagava, Sujata Mani, Sikandar I Mulla, Ganesh Dattatraya Saratale
This study deals the biodegradation of crystal violet dye by a ligninolytic enzyme producing bacterium isolated from textile wastewater that was characterized and identified as Aeromonas hydrophila based on the 16 S rRNA gene sequence analysis. The degradation of crystal violet dye was studied under different environmental and nutritional conditions, and results showed that the isolated bacterium was effective to decolourize 99% crystal violet dye at pH 7 and temperature 35 °C in presence of sucrose and yeast extract as C and N source, respectively...
March 15, 2018: Ecotoxicology and Environmental Safety
Wen-Long Liu, Xi-Li Zhang, Shi-Qi Fan, Jun-Ping Zhu, Hui-Hui Liang, Yu-Tian Zhang, Mei-Feng Xiao, Yan Zeng, Hong He, Fu-Yuan He, Xiao-Qin Xiao
BACKGROUND: Drugs derived from botany have been playing essential role in both clinical treatment and pharmaceutical industry, unfortunately our worry is still that its quality and therapeutic efficacy are inconsistent. Recently many scientists launched a new project on quality (Q)-marker of medicinal herbs, this study was thus designed to generate a novel concept of quality (Q)-markers: molecular connectivity index (MCI), and to test and verify the new concept of molecular connectivity index (MCI)...
March 8, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
M Kim, M Kim, H J Yoo, A Lee, S Jeong, J H Lee
We aimed to evaluate the longitudinal interaction effects between the minor allele of FADS1 rs174547 and overweight on n-3 and n-6 long-chain polyunsaturated fatty acid (PUFA) levels and pulse wave velocity (PWV). Plasma PUFA levels were measured via GC-MS, and arterial stiffness was determined as brachial-ankle PWV (ba-PWV) at baseline and after a mean follow-up of 3 years. The FADS1 rs174547 T > C genotype was analyzed. At 3-years of follow-up, after adjustment for age, sex, smoking and drinking, there were interaction effects between the FADS1 rs174547 T > C genotype and baseline BMI on the changes (from baseline) in plasma arachidonic acid (AA) levels, in the eicosapentaenoic acid (EPA)/AA ratio, and in ba-PWV (p for interaction = 0...
March 2018: Prostaglandins, Leukotrienes, and Essential Fatty Acids
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