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claudin-5 gene

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https://www.readbyqxmd.com/read/29747348/-cerebral-cavernous-malformation-3-gene-deficiency-promotes-early-changes-in-alzheimer-disease-like-lesions-induced-by-low-lead-exposure
#1
S Wu, D Xia, H X Liu, H P Zhao, X H Wang, Y F Gao, Z Q Zhao, C Xiao, X M Xing, Y He
Objective: To investigate the effects of cerebral cavernous malformation 3 (CCM3) gene knockout on the lead exposure-induced blood-brain barrier malfunction in mice brain, and the relationship between CCM3 knockout and the Alzheimer's disease (AD). Methods: Wide type (WT) mice and CCM3(+)/- mice were divided into 4 groups, control group and lead exposed group in WT as well as CCM3(+/-) mice. Lead exposed groups were treated with 0.05% lead acetate in drinking water for 12 weeks, while control group drink deionized water freely...
May 6, 2018: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/29745557/-effects-of-silencing-snail1-gene-on-the-expression-of-tight-junction-proteins-and-the-migration-ability-of-hep-2-cells
#2
Shuangfeng Liu, Yang Shen, Tang Feng, Xiaoheng Liu
To investigate the effects of Snail1 gene silence on the expression of tight junction proteins and the migration ability of Hep-2 cells, Hep-2 cells were transfected with plasmids which is containing the shRNA of Snail1 gene, and cultured till the cells could be passaged stably (named Sh-snail1 cells). The expression of tight junction proteins (ZO-1, Occludin, Claudin-5) were detected by Western blot. The migration ability of Sh-snail1 cells was investigated by wound healing assay, and the protein expression of members of RhoGTPase family (RhoA, Cdc42) was detected by Western blot, which is closely related to the migration ability...
August 1, 2017: Sheng Wu Yi Xue Gong Cheng Xue za Zhi, Journal of Biomedical Engineering, Shengwu Yixue Gongchengxue Zazhi
https://www.readbyqxmd.com/read/29731129/early-onset-pediatric-atopic-dermatitis-is-characterized-by-t-h-2-t-h-17-t-h-22-centered-inflammation-and-lipid-alterations
#3
Patrick M Brunner, Ariel Israel, Ning Zhang, Alexandra Leonard, Huei-Chi Wen, Thy Huynh, Gary Tran, Sarah Lyon, Giselle Rodriguez, Supriya Immaneni, Annette Wagner, Xiuzhong Zheng, Yeriel D Estrada, Hui Xu, James G Krueger, Amy S Paller, Emma Guttman-Yassky
BACKGROUND: Although atopic dermatitis (AD) often starts in early childhood, detailed tissue profiling of early-onset AD in children is lacking, hindering therapeutic development for this patient population with a particularly high unmet need for better treatments. OBJECTIVE: We sought to globally profile the skin of infants with AD compared with that of adults with AD and healthy control subjects. METHODS: We performed microarray, RT-PCR, and fluorescence microscopy studies in infants and young children (<5 years old) with early-onset AD (<6 months disease duration) compared with age-matched control subjects and adults with longstanding AD...
April 19, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29722942/-role-of-tgf-%C3%AE-1-in-sertoli-cells-and-tight-junction
#4
Jiang-Tao Lou, Ren-Xiong Wei, Lang-Lang Yu, Jian-Wei Chen, Yun Cui
Objective: To explore the role of TGF-β1 in the proliferation and apoptosis of Sertoli cells and its effect on the expressions of tight junction-related proteins and genes in rats. METHODS: Rat Sertoli cells were isolated in vitro, primarily cultured, and divided into groups A (blank control), B (TGF-β1 receptor blocker), C (TGF-β1), and D (TGF-β1 + receptor blocker). The proliferation and apoptosis of the cells were detected by CCK-8 and flow cytometry, respectively...
June 2017: Zhonghua Nan Ke Xue, National Journal of Andrology
https://www.readbyqxmd.com/read/29696536/transgenic-up-regulation-of-claudin-6-decreases-fine-diesel-particulate-matter-dpm-induced-pulmonary-inflammation
#5
Joshua B Lewis, Jared S Bodine, Jason R Gassman, Samuel Arce Muñoz, Dallin C Milner, Todd M Dunaway, Kaleb M Egbert, Troy D Monson, Dallin S Broberg, Juan A Arroyo, Paul R Reynolds
Claudin-6 (Cldn6) is a tetraspanin transmembrane protein that contributes to tight junctional complexes and has been implicated in the maintenance of lung epithelial barriers. In the present study, we tested the hypothesis that genetic up-regulation of Cldn-6 influences inflammation in mice exposed to short-term environmental diesel particulate matter (DPM). Mice were subjected to ten exposures of nebulized DPM (PM2.5) over a period of 20 days via a nose-only inhalation system (Scireq, Montreal, Canada). Using real-time RT-PCR, we discovered that the Cldn6 gene was up-regulated in control mice exposed to DPM and in lung-specific transgenic mice that up-regulate Cldn-6 (Cldn-6 TG)...
April 25, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29689364/reversible-renal-glomerular-dysfunction-in-guinea-pigs-exposed-to-glutaraldehyde-polymerized-cell-free-hemoglobin
#6
Xiaoyuan Zhang, Matthew C Williams, Otgonchimeg Rentsendorj, Felice D'Agnillo
Chemically modified hemoglobin (Hb)-based oxygen carriers are promising oxygen replacement therapeutics however their potential renal effects are not fully understood. Using a guinea pig exchange transfusion model, we examined the effects of glutaraldehyde-polymerized bovine hemoglobin (HbG) on the permeability and integrity of the glomerular filtration barrier (GFB), which is comprised of podocytes, fenestrated endothelium, and the glomerular basement membrane. HbG induced marked proteinuria characterized in part by the loss of high molecular weight proteins, including albumin, immunoglobulin, and transferrin, at 4 and 12 h post-infusion that resolved by 72 h...
April 22, 2018: Toxicology
https://www.readbyqxmd.com/read/29664376/rickettsia-asembonensis-characterization-by-multilocus-sequence-typing-of-complete-genes-peru
#7
Steev Loyola, Carmen Flores-Mendoza, Armando Torre, Claudine Kocher, Melanie Melendrez, Alison Luce-Fedrow, Alice N Maina, Allen L Richards, Mariana Leguia
While studying rickettsial infections in Peru, we detected Rickettsia asembonensis in fleas from domestic animals. We characterized 5 complete genomic regions (17kDa, gltA, ompA, ompB, and sca4) and conducted multilocus sequence typing and phylogenetic analyses. The molecular isolate from Peru is distinct from the original R. asembonensis strain from Kenya.
May 2018: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29659957/dietary-fermentable-fibers-attenuate-chronic-kidney-disease-in-mice-by-protecting-the-intestinal-barrier
#8
Tran Van Hung, Takuya Suzuki
Background: Chronic kidney disease (CKD) is a worldwide health problem. Although the pathogenesis of CKD is still unclear, recent studies suggest that systemic inflammation caused by a dysregulated microflora and an impaired intestinal barrier is involved in CKD development. Objective: We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice...
April 1, 2018: Journal of Nutrition
https://www.readbyqxmd.com/read/29652749/sera-from-children-after-cardiopulmonary-bypass-reduces-permeability-of-capillary-endothelial-cell-barriers
#9
Richard W Pierce, Riad Abou Zahr, Sarah Kandil, E Vincent S Faustino, Jordan S Pober
OBJECTIVES: Children undergoing cardiopulmonary bypass develop clinically impactful capillary leak of unclear etiology. A widely held hypothesis that exposure of circulating cells to the cardiopulmonary bypass circuit induces the release of inflammatory mediators that act to disrupt intercellular junctions of capillary endothelial cells inducing paracellular capillary leak either directly or through new gene expression. DESIGN: Cohort study. SETTING: Tertiary pediatric hospital...
April 12, 2018: Pediatric Critical Care Medicine
https://www.readbyqxmd.com/read/29650692/microrna-mediated-therapy-modulating-blood-brain-barrier-disruption-improves-vascular-cognitive-impairment
#10
Kensuke Toyama, Joshua M Spin, Alicia C Deng, Ting-Ting Huang, Ke Wei, Markus U Wagenhäuser, Takuya Yoshino, Huy Nguyen, Joscha Mulorz, Soumajit Kundu, Uwe Raaz, Matti Adam, Isabel N Schellinger, Ann Jagger, Philip S Tsao
OBJECTIVE: There are currently no effective treatments for the prevention of dementia associated with vascular cognitive impairment. MicroRNAs regulate gene expression at the post-transcriptional level and play key roles in vascular disorders. TNFα (tumor necrosis factor-α) regulates blood-brain barrier breakdown through modification of cerebral tight junctions. Here, we sought key TNFα-responsive microRNAs that might influence blood-brain barrier breakdown via cerebral tight junction disruption in vascular cognitive impairment...
April 12, 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29622432/sirtuin-5-as-a-novel-target-to-blunt-blood-brain-barrier-damage-induced-by-cerebral-ischemia-reperfusion-injury
#11
Candela Diaz-Cañestro, Mario Merlini, Nicole R Bonetti, Luca Liberale, Patricia Wüst, Sylvie Briand-Schumacher, Jan Klohs, Sara Costantino, Melroy Miranda, Gabriele Schoedon-Geiser, Gerd A Kullak-Ublick, Alexander Akhmedov, Francesco Paneni, Jürg H Beer, Thomas F Lüscher, Giovanni G Camici
BACKGROUND: In acute ischemic stroke (AIS) patients, impaired blood-brain barrier (BBB) integrity is associated with hemorrhagic transformation and worsened outcome. Yet, the mechanisms underlying these relationships are poorly understood and consequently therapeutic strategies are lacking. This study sought to determine whether SIRT5 contributes to BBB damage following I/R brain injury. METHODS AND RESULTS: SIRT5 knockout (SIRT5-/- ) and wild type (WT) mice underwent transient middle cerebral artery (MCA) occlusion (tMCAO) followed by 48h of reperfusion...
June 1, 2018: International Journal of Cardiology
https://www.readbyqxmd.com/read/29550680/effect-of-different-dietary-non-starch-fiber-fractions-on-growth-performance-nutrient-digestibility-and-intestinal-development-in-weaned-pigs
#12
Xiying Wu, Daiwen Chen, Bing Yu, Yuheng Luo, Ping Zheng, Xiangbing Mao, Jie Yu, Jun He
OBJECTIVES: This study explored the effects of different fiber fractions on growth performance, nutrient digestibility and intestinal development in a porcine model. METHODS: Blood and tissue samples were collected from thirty-two weaned pigs fed with control diet (CON) or diet containing 5 % cellulose (CEL), xylan (XYL) or β-glucan (GLU). RESULTS: This reserch showed that GLU supplementation decreased the growth performance of pigs (P <0...
February 6, 2018: Nutrition
https://www.readbyqxmd.com/read/29533123/protective-effects-of-sulforaphane-in-experimental-vascular-cognitive-impairment-contribution-of-the-nrf2-pathway
#13
Leilei Mao, Tuo Yang, Xin Li, Xia Lei, Yang Sun, Yongfang Zhao, Wenting Zhang, Yanqin Gao, Baoliang Sun, Feng Zhang
The major pathophysiological process of vascular cognitive impairment (VCI) is chronic cerebral ischemia, which causes disintegration of the blood-brain barrier (BBB), neuronal death, and white matter injury. This study aims to test whether sulforaphane (Sfn), a natural activator of nuclear factor erythroid 2-related factor 2 (Nrf2), reduces the chronic ischemic injury and cognitive dysfunction after VCI. Experimental VCI was induced in rats by permanent occlusion of both common carotid arteries for six weeks...
January 1, 2018: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/29508680/medium-chain-tag-improve-intestinal-integrity-by-suppressing-toll-like-receptor-4-nucleotide-binding-oligomerisation-domain-proteins-and-necroptosis-signalling-in-weanling-piglets-challenged-with-lipopolysaccharide
#14
Xiao Xu, Shaokui Chen, Haibo Wang, Zhixiao Tu, Shuhui Wang, Xiuying Wang, Huiling Zhu, Chunwei Wang, Jiadong Zhu, Yulan Liu
This study was conducted to evaluate whether medium-chain TAG (MCT) could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury by regulating intestinal epithelial inflammatory response, as well as necroptosis. A total of twenty-four weanling piglets were randomly allotted to one of four treatments in a 2×2 factorial arrangement including diet type (5 % maize oil v. 4 % MCT+1 % maize oil) and immune stress (saline v. E. coli LPS). The piglets were fed diets containing maize oil or MCT for 21 d...
May 2018: British Journal of Nutrition
https://www.readbyqxmd.com/read/29488037/severe-intestinal-inflammation-in-the-small-intestine-of-mice-induced-by-controllable-deletion-of-claudin-7
#15
Wen-Jing Li, Chang Xu, Kun Wang, Teng-Yan Li, Xiao-Nan Wang, Hui Yang, Tiaosi Xing, Wen-Xia Li, Yan-Hua Chen, Hong Gao, Lei Ding
BACKGROUND: As a potential tumor suppressor gene, Claudin-7 (Cldn7), which is a component of tight junctions, may play an important role in colorectal cancer occurrence and development. AIMS: To generate a knockout mouse model of inducible conditional Cldn7 in the intestine and analyze the phenotype of the mice after induction with tamoxifen. METHODS: We constructed Cldn7-flox transgenic mice and crossed them with Villin-CreERT2 mice. The Cldn7 inducible conditional knockout mice appeared normal and were well developed at birth...
May 2018: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/29482498/high-level-cytoplasmic-claudin-3-expression-is-an-independent-predictor-of-poor-survival-in-triple-negative-breast-cancer
#16
Anniina Jääskeläinen, Ylermi Soini, Arja Jukkola-Vuorinen, Päivi Auvinen, Kirsi-Maria Haapasaari, Peeter Karihtala
BACKGROUND: The subtype of claudin-low breast cancer can be reliably determined only by gene-expression profiling. Attempts have been made to develop immunohistochemical surrogates, which nearly always focus on membranous claudin expression. METHODS: We assessed the immunohistochemical expression of both membranous and cytoplasmic claudins 3, 4 and 7 in a series of 197 non-metastatic breast cancers, enriched with triple-negative breast cancers (TNBCs; 60%). The expression of epithelial-to-mesenchymal transition-regulating transcription factors Sip1, Zeb1 and vimentin had previously been determined in the same material...
February 27, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29470360/oral-supplementation-with-bovine-colostrum-prevents-septic-shock-and-brain-barrier-disruption-during-bloodstream-infection-in-preterm-newborn-pigs
#17
Anders Brunse, Päivi Worsøe, Susanne Elisabeth Pors, Kerstin Skovgaard, Per Torp Sangild
Preterm infants have increased risk of neonatal sepsis, potentially inducing brain injury, and they may benefit from early initiation of enteral milk feeding. Using preterm pigs as models, we hypothesized that early provision of bovine colostrum to parentally nourished newborns protects against sepsis and neuroinflammation during bloodstream infection. Preterm newborn pigs were administered 10 CFU/kg of intra-arterial Staphylococcus epidermidis (SE, an opportunistic pathogen often causing sepsis in preterm infants), followed by administration of total parenteral nutrition (TPN, SE + TPN, n = 15) or oral provision of bovine colostrum with supplementary parenteral nutrition (SE + COL, n = 14), and compared with uninfected, TPN-nourished controls (CON + TPN, n = 11)...
February 21, 2018: Shock
https://www.readbyqxmd.com/read/29467620/mir-429-regulated-by-endothelial-monocyte-activating-polypeptide-ii-emap-ii-influences-blood-tumor-barrier-permeability-by-inhibiting-the-expressions-of-zo-1-occludin-and-claudin-5
#18
Liangyu Chen, Yixue Xue, Jian Zheng, Xiaobai Liu, Jing Liu, Jiajia Chen, Zhen Li, Zhuo Xi, Hao Teng, Ping Wang, Libo Liu, Yunhui Liu
The blood-tumor barrier (BTB) hinders delivery of chemotherapeutic drugs to tumors in the brain; previous studies have shown that the BTB can be selectively opened by endothelial monocyte activating polypeptide-II (EMAP-II), but the specific mechanism involved remains elusive. In this study, we found that microRNA-429 (miR-429) expression in glioma vascular endothelial cells (GECs) was far lower than in human brain microvascular endothelial cells (ECs). miR-429 had lower expression in GECs and glioma tissues compared to ECs or normal tissues of the brain...
2018: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/29424664/chemotaxis-like-pathway-of-azorhizobium-caulinodans-controls-flagella-driven-motility-which-regulates-biofilms-formation-exopolysaccharide-biosynthesis-and-competitive-nodulation
#19
Zhihong Xie, Wei Liu, Yu Sun, Rimin Shen, Xiaoxiao Dang, Xiaolin Liu, Fu Sui, Yan Li, Zhenpeng Zhang, Gladys Alexandre, Claudine Elmerich
The genome of the Azorhizobium caulinodans ORS571 contains a unique chemotaxis gene cluster (che) including 5 chemotaxis genes: cheAWY1BR. Analysis of the role of the chemotaxis cluster of A. caulinodans, using deletion mutant strains revealed that CheA or Che signaling pathway controls chemotaxis behavior and flagella-driven motility, and plays important roles in biofilms formation and production of extracellular polysaccharides. Furthermore, the deletion mutants (ΔcheA and ΔcheA-R) were defective in competitive adsorption and colonization on the root surface of host plants...
February 9, 2018: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/29422769/a-genome-wide-association-study-of-corneal-astigmatism-the-cream-consortium
#20
Rupal L Shah, Qing Li, Wanting Zhao, Milly S Tedja, J Willem L Tideman, Anthony P Khawaja, Qiao Fan, Seyhan Yazar, Katie M Williams, Virginie J M Verhoeven, Jing Xie, Ya Xing Wang, Moritz Hess, Stefan Nickels, Karl J Lackner, Olavi Pärssinen, Juho Wedenoja, Ginevra Biino, Maria Pina Concas, André Uitterlinden, Fernando Rivadeneira, Vincent W V Jaddoe, Pirro G Hysi, Xueling Sim, Nicholas Tan, Yih-Chung Tham, Sonoko Sensaki, Albert Hofman, Johannes R Vingerling, Jost B Jonas, Paul Mitchell, Christopher J Hammond, René Höhn, Paul N Baird, Tien-Yin Wong, Chinfsg-Yu Cheng, Yik Ying Teo, David A Mackey, Cathy Williams, Seang-Mei Saw, Caroline C W Klaver, Jeremy A Guggenheim, Joan E Bailey-Wilson
Purpose: To identify genes and genetic markers associated with corneal astigmatism. Methods: A meta-analysis of genome-wide association studies (GWASs) of corneal astigmatism undertaken for 14 European ancestry (n=22,250) and 8 Asian ancestry (n=9,120) cohorts was performed by the Consortium for Refractive Error and Myopia. Cases were defined as having >0.75 diopters of corneal astigmatism. Subsequent gene-based and gene-set analyses of the meta-analyzed results of European ancestry cohorts were performed using VEGAS2 and MAGMA software...
2018: Molecular Vision
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