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liver decellularization

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https://www.readbyqxmd.com/read/29701314/reconstruction-of-structure-and-function-in-tissue-engineering-of-solid-organs-toward-simulation-of-natural-development-based-on-decellularization
#1
REVIEW
Chen-Xi Zheng, Bing-Dong Sui, Cheng-Hu Hu, Xin-Yu Qiu, Pan Zhao, Yan Jin
Failure of solid organs, such as heart, liver and kidney, remains a major cause of the world's mortality due to critical shortage of donor organs. Tissue engineering, which uses elements including cells, scaffolds and growth factors to fabricate functional organs in vitro, is a promising strategy to mitigate the scarcity of transplantable organs. Within recent years, different construction strategies which guide the combination of tissue engineering elements have been applied in solid organ tissue engineering and have achieved much progress...
April 27, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29678581/molecular-and-histological-study-on-the-effects-of-non-thermal-irreversible-electroporation-on-the-liver
#2
Yanfang Zhang, Chenang Lyu, Yu Liu, Yanpeng Lv, Tammy T Chang, Boris Rubinsky
Non-thermal irreversible electroporation (NTIRE) is a biophysical phenomenon in which certain electric fields delivered across the cell membrane in tissue, cause cell death, without affecting the extracellular matrix. "Minimally invasive regenerative surgery" is a new medical modality for treatment of end-stage organ or tissue failure in which exogenous cells are implanted in a decellularized niche in tissue, formed by the delivery of NTIRE electric fields across a targeted volume of tissue. We anticipate that the success of the procedure will depend on the time of implantation relative to the application of NTIRE...
June 7, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29605076/future-approaches-and-therapeutic-modalities-for-acute-liver-failure
#3
REVIEW
Pavan Patel, Nneoma Okoronkwo, Nikolaos T Pyrsopoulos
The current gold standard for the management of acute liver failure is liver transplantation. However, because of organ shortages, other modalities of therapy are necessary as a possible bridge. This article discusses the current modalities as well as the future management of acute liver failure. Liver assist devices, hepatocyte transplantation, stem cell transplant, organogenesis, and repopulation of decellularized organs are discussed.
May 2018: Clinics in Liver Disease
https://www.readbyqxmd.com/read/29587547/silver-nanoparticles-improve-structural-stability-and-biocompatibility-of-decellularized-porcine-liver
#4
Tarek Saleh, Ebtehal Ahmed, Lina Yu, Kamal Hussein, Kyung-Mee Park, Yun-Suk Lee, Byung-Jae Kang, Ki-Young Choi, Sooyoung Choi, Kyung-Sun Kang, Heung-Myong Woo
No ideal cross-linking agent has been identified for decellularized livers (DLs) yet. In this study, we evaluated structural improvements and biocompatibility of porcine DLs after cross-linking with silver nanoparticles (AgNPs). Porcine liver slices were decellularized and then loaded with AgNPs (100 nm) after optimization of the highest non-toxic concentration (5 µg/mL) using Human hepatocellular carcinoma (HepG2) and EAhy926 human endothelial cell lines. The cross-linking effect of AgNPs was evaluated and compared to that of glutaraldehyde and ethyl carbodiimide hydrochloride and N-hydroxysuccinimide...
March 27, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29390876/decellularization-and-solubilization-of-porcine-liver-for-use-as-a-substrate-for-porcine-hepatocyte-culture-method-optimization-and-comparison
#5
Ramon E Coronado, Maria Somaraki-Cormier, Shanmugasundaram Natesan, Robert J Christy, Joo L Ong, Glenn A Halff
Biologic substrates, prepared by decellularizing and solubilizing tissues, have been of great interest in the tissue engineering field because of the preservation of complex biochemical constituents found in the native extracellular matrix (ECM). The integrity of the ECM is critical for cell behavior, adhesion, migration, differentiation, and proliferation that in turn affect homeostasis and tissue regeneration. Previous studies have shown that various processing methods have a distinctive way of affecting the composition of the decellularized ECM...
December 2017: Cell Transplantation
https://www.readbyqxmd.com/read/29377912/recellularization-of-rat-liver-an-in-vitro-model-for-assessing-human-drug-metabolism-and-liver-biology
#6
Matthew J Robertson, Benjamin Soibam, Jacqueline G O'Leary, Luiz C Sampaio, Doris A Taylor
Liver-like organoids that recapitulate the complex functions of the whole liver by combining cells, scaffolds, and mechanical or chemical cues are becoming important models for studying liver biology and drug metabolism. The advantages of growing cells in three-dimensional constructs include enhanced cell-cell and cell-extracellular matrix interactions and preserved cellular phenotype including, prevention of de-differentiation. In the current study, biomimetic liver constructs were made via perfusion decellularization of rat liver, with the goal of maintaining the native composition and structure of the extracellular matrix...
2018: PloS One
https://www.readbyqxmd.com/read/29338127/establishment-of-practical-recellularized-liver-graft-for-blood-perfusion-using-primary-rat-hepatocytes-and-liver-sinusoidal-endothelial-cells
#7
H Kojima, K Yasuchika, K Fukumitsu, T Ishii, S Ogiso, Y Miyauchi, R Yamaoka, T Kawai, H Katayama, E Y Yoshitoshi-Uebayashi, S Kita, K Yasuda, N Sasaki, J Komori, S Uemoto
Tissue decellularization produces a three-dimensional scaffold that can be used to fabricate functional liver grafts following recellularization. Inappropriate cell distribution and clotting during blood perfusion hinder the practical use of recellularized livers. Here we aimed to establish a seeding method for the optimal distribution of parenchymal and endothelial cells, and to evaluate the effect of liver sinusoidal endothelial cells (LSECs) in the decellularized liver. Primary rat hepatocytes and LSECs were seeded into decellularized whole-liver scaffolds via the biliary duct and portal vein, respectively...
January 16, 2018: American Journal of Transplantation
https://www.readbyqxmd.com/read/29247536/three-dimensional-liver-derived-extracellular-matrix-hydrogel-promotes-liver-organoids-function
#8
Mona Saheli, Mohammadmajid Sepantafar, Behshad Pournasr, Zahra Farzaneh, Massoud Vosough, Abbas Piryaei, Hossein Baharvand
An important advantage of employing extracellular matrix (ECM)-derived biomaterials in tissue engineering is the ability to tailor the biochemical and biophysical microenvironment of the cells. This study aims to assess whether three-dimensional (3D) liver-derived ECM hydrogel (LEMgel) promotes physiological function of liver organoids generated by self-organization of human hepatocarcinoma cells together with human mesenchymal and endothelial cells. We have optimized the decellularization method to fabricate liver ECM derived from sheep to preserve the greatest content of glycosaminoglycans, collagen, laminin, and fibronectin in produced LEMgel...
December 16, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29222839/decellularized-human-liver-extracellular-matrix-hdlm-mediated-hepatic-differentiation-of-human-induced-pluripotent-stem-cells-hipscs
#9
Maria Jaramillo, Heidi Yeh, Martin L Yarmush, Basak E Uygun
Liver tissue engineering has emerged as a promising approach in organ transplantation but has been hampered by the lack of a reliable and readily available cell source. Human induced pluripotent stem cells hiPSCs have been highlighted as a desirable source, due to their differentiation potential, ability to self-renew, and the possibility of making patient-specific cells. We developed a decellularization protocol that efficiently removes cellular material while retaining extracellular matrix components. Subsequently, hiPSCs were differentiated on decellularized human liver extracellular matrix (hDLM) scaffolds using an established hepatic differentiation protocol...
December 8, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29221151/collagen-i-promotes-hepatocellular-carcinoma-cell-proliferation-by-regulating-integrin-%C3%AE-1-fak-signaling-pathway-in-nonalcoholic-fatty-liver
#10
Xinglong Zheng, Wenyan Liu, Junxi Xiang, Peng Liu, Mengyun Ke, Bo Wang, Rongqian Wu, Yi Lv
Nonalcoholic fatty liver disease (NAFLD) has become a major risk factor for hepatocellular carcinoma (HCC) worldwide. However, the underlying mechanism remains insufficiently elucidated. The expression of Collagen I, an important component of extracellular matrix (ECM), was increased during the progression from simple steatosis to NASH. The purpose of this study was to investigate the role of Collagen I in NAFLD-related HCC. To study this, the decellularized liver matrix, which preserves the pathological changes of ECM, was prepared from the human fatty liver (FLM) and human normal liver (NLM)...
November 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29211918/decellularized-human-placenta-supports-hepatic-tissue-and-allows-rescue-in-acute-liver-failure
#11
Zurab Kakabadze, Anne Kakabadze, David Chakhunashvili, Lika Karalashvili, Ekaterine Berishvili, Yogeshwar Sharma, Sanjeev Gupta
Tissue engineering with scaffolds to form transplantable organs is of wide interest. Decellularized tissues have been tested for this purpose, although supplies of healthy donor tissues, vascular recellularization for perfusion and tissue homeostasis in engineered organs pose challenges. We hypothesized decellularized human placenta will be suitable for tissue engineering. The universal availability and unique structures of placenta for accommodating tissue, including presence of embedded vessels, were major attractions...
December 6, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29210278/decellularized-liver-matrix-modified-cryogel-scaffolds-as-potential-hepatocyte-carriers-in-bioartificial-liver-support-systems-and-implantable-liver-constructs
#12
Apeksha Damania, Anupam Kumar, Arun K Teotia, Haruna Kimura, Masamichi Kamihira, Hiroyuki Ijima, Shiv Kumar Sarin, Ashok Kumar
Recent progress in the use of decellularized organ scaffolds as regenerative matrices for tissue engineering holds great promise in addressing the issue of donor organ shortage. Decellularization preserves the mechanical integrity, composition, and microvasculature critical for zonation of hepatocytes in the liver. Earlier studies have reported the possibility of repopulating decellularized matrices with hepatic cell lines or stem cells to improve liver regeneration. In this work, we study the versatility of the decellularized liver matrix as a substrate coating of three-dimensional cryogel scaffolds...
January 10, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29204185/bioengineering-considerations-in-liver-regenerative-medicine
#13
REVIEW
Ogechi Ogoke, Janet Oluwole, Natesh Parashurama
Background: Liver disease contributes significantly to global disease burden and is associated with rising incidence and escalating costs. It is likely that innovative approaches, arising from the emerging field of liver regenerative medicine, will counter these trends. Main body: Liver regenerative medicine is a rapidly expanding field based on a rich history of basic investigations into the nature of liver structure, physiology, development, regeneration, and function...
2017: Journal of Biological Engineering
https://www.readbyqxmd.com/read/29157906/novel-decellularized-liver-matrix-alginate-hybrid-gel-beads-for-the-3d-culture-of-hepatocellular-carcinoma-cells
#14
Dongsheng Sun, Yang Liu, Huihui Wang, Fei Deng, Ying Zhang, Shan Zhao, Xiaojun Ma, Huijian Wu, Guangwei Sun
Developing reliable three-dimensional (3D) cell culture systems that can mimic native tumor microenvironments is necessary for investigating the mechanism of hepatocellular carcinoma (HCC) metastasis and screen therapeutic drugs. In the present study, we developed decellularized liver matrix-alginate (DLM-ALG) hybrid gel beads. DLM powder was prepared by optimized decellularization methods and liquid nitrogen grinding. DLM-ALG beads were generated by dropping alginate solution containing DLM powder into a gelling bath...
April 1, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29152658/hepatic-differentiation-of-mouse-bone-marrow%C3%A2-derived-mesenchymal-stem-cells-using-a-novel-3d-culture-system
#15
Qiong Wu, Jing Tang, Yi Li, Li Li, Yujia Wang, Ji Bao, Hong Bu
The development of novel culture systems that mimic the in vivo microenvironment may be beneficial for inducing the differentiation of stem cells and promoting liver function. In the present study, spheroid cultures and decellularized liver scaffolds (DLSs) were utilized to obtain differentiated hepatocyte‑like cells. Mouse bone marrow (BM)‑derived mesenchymal stem cells (MSCs) self‑aggregated into spheroids under low‑attachment conditions and implanted into the DLSs via a negative pressure suction device...
December 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29104526/-tissue-papers-from-organ-specific-decellularized-extracellular-matrices
#16
Adam E Jakus, Monica M Laronda, Alexandra S Rashedi, Christina M Robinson, Chris Lee, Sumanas W Jordan, Kyle E Orwig, Teresa K Woodruff, Ramille N Shah
Using an innovative, tissue-independent approach to decellularized tissue processing and biomaterial fabrication, the development of a series of "tissue papers" derived from native porcine tissues/organs (heart, kidney, liver, muscle), native bovine tissue/organ (ovary and uterus), and purified bovine Achilles tendon collagen as a control from decellularized extracellular matrix particle ink suspensions cast into molds is described. Each tissue paper type has distinct microstructural characteristics as well as physical and mechanical properties, is capable of absorbing up to 300% of its own weight in liquid, and remains mechanically robust ( E = 1-18 MPa) when hydrated; permitting it to be cut, rolled, folded, and sutured, as needed...
September 13, 2017: Advanced Functional Materials
https://www.readbyqxmd.com/read/29101679/simple-and-quick-method-to-obtain-a-decellularized-functional-liver-bioscaffold
#17
Matteo Ghiringhelli, Alessandro Zenobi, Stefano Brizzola, Fulvio Gandolfi, Valentino Bontempo, Sandro Rossi, Tiziana A L Brevini, Fabio Acocella
The development of new approaches for organ transplantation has become crucial in the last years. In particular, organ engineering, involving the preparation of acellular matrices that provide a natural habitat for reseeding with an appropriate population of cells, is an attractive although technically demanding approach. We here describe a method that allows for the derivation of functional in vitro hepatic organoids and that does not require a previous selection of all the parenchymal hepatocytes and non-parenchymal cells, namely, Kupffer cells, liver endothelial cells, and hepatic stellate cells...
November 4, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29101678/liver-bioengineering-using-decellularized-whole-liver-scaffolds
#18
Iris Pla-Palacín, Pilar Sainz-Arnal, Sara Morini, Manuel Almeida, Pedro M Baptista
Currently, due to the progress made in the field of regenerative medicine, whole-organ bioengineering is becoming a valid alternative to cope with the shortages of organs for transplantation. In this chapter, we describe the main techniques carried out for pig liver bioengineering, which serves as an essential model for future human liver bioengineering. These include porcine whole-liver decellularization, endothelial and mesenchymal stem cell isolation, porcine ES-derived hepatoblasts, and scaffold recellularization using a bioreactor perfusion system...
November 4, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29052367/decellularized-caprine-liver-extracellular-matrix-as-a-2d-substrate-coating-and-3d-hydrogel-platform-for-vascularized-liver-tissue-engineering
#19
Tarun Agarwal, Rajan Narayan, Somnath Maji, Sudip Kumar Ghosh, Tapas Kumar Maiti
Development of a vascularized liver tissue construct is a need of an hour to circumvent the current demand of liver transplantation in health care sector. An appropriate matrix must support liver cell viability, functionality, and development of microvasculature. With this perspective, here, we report the use of decellularized caprine liver extracellular matrix (CLECM) derived hydrogel for tissue engineering applications. First, CLECM was used as a substrate coating material for 2D hepatocyte culture. HepG2 cells cultured on CLECM-coated surface showed higher albumin, urea, glycogen, and GAGs synthesis in comparison with collagen-coated surface (taken as control for the study)...
March 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29017397/liver-scaffolds-support-survival-and-metabolic-function-of-multilineage-neonatal-allogenic-cells
#20
Wessam Hassanein, Arielle Cimeno, Avraham Werdesheim, Bryan Buckingham, Joshua Harrison, Mehmet C Uluer, Ali Khalifeh, Carlos Rivera-Pratt, Stephen Klepfer, Jhade D Woodall, Urmil Dhru, Elliot Bromberg, Dawn Parsell, Cinthia Drachenberg, Rolf N Barth, John C LaMattina
Organ scaffold bioengineering is currently limited by the inability to effectively repopulate the scaffold with appropriately distributed functional cells. We examined the feasibility of a decellularized liver scaffold to support the growth and function of multilineage allogenic cells derived from either adult or neonatal liver cells. Cell slurries from neonatal and adult rat livers containing hepatocytes, cholangiocytes, and endothelial cells were introduced into decellularized adult rat liver scaffolds via the bile duct...
November 30, 2017: Tissue Engineering. Part A
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