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liver decellularization

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https://www.readbyqxmd.com/read/28600910/dried-human-skin-fibroblasts-as-a-new-substratum-for-functional-culture-of-hepatic-cells
#1
Agnieszka Wencel, Karolina Ewa Zakrzewska, Anna Samluk, Bartłomiej Henryk Noszczyk, Dorota Genowefa Pijanowska, Krzysztof Dariusz Pluta
The primary hepatocytes culture is still one of the main challenges in toxicology studies in the drug discovery process, development of in vitro models to study liver function, and cell-based therapies. Isolated hepatocytes display a rapid decline in viability and liver-specific functions including albumin production, conversion of ammonia to urea, and activity of the drug metabolizing enzymes. A number of methods have been developed in order to maintain hepatocytes in their highly differentiated state in vitro...
2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/28556592/the-effect-of-riboflavin-uva-cross-linking-on-anti-degeneration-and-promoting-angiogenic-capability-of-decellularized-liver-matrix
#2
Junxi Xiang, Peng Liu, Xinglong Zheng, Dinghui Dong, Shujuan Fan, Jian Dong, Xufeng Zhang, Xuemin Liu, Bo Wang, Yi Lv
Weak mechanical property and unstable degradation rate limited the application of decellularized liver matrix in tissue engineering. The aim of this study was to explore a new method for improving the mechanical properties, anti-degeneration and angiogenic capability of decellularized liver matrix. This was achieved by a novel approach using riboflavin/ultraviolet A treatment to induce collagen cross-linking of decellularized matrix. Histological staining and scanning electron microscope showed that the diameter of cross-linked fibers significantly increased compared with the control group...
May 27, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28543403/decellularized-human-liver-is-too-heterogeneous-for-designing-a-generic-extracellular-matrix-mimic-hepatic-scaffold
#3
Giorgio Mattei, Chiara Magliaro, Andrea Pirone, Arti Ahluwalia
Decellularized human livers are considered the perfect extracellular matrix (ECM) surrogate because both three-dimensional architecture and biological features of the hepatic microenvironment are thought to be preserved. However, donor human livers are in chronically short supply, both for transplantation or as decellularized scaffolds, and will become even scarcer as life expectancy increases. It is hence of interest to determine the structural and biochemical properties of human hepatic ECM to derive design criteria for engineering biomimetic scaffolds...
May 23, 2017: Artificial Organs
https://www.readbyqxmd.com/read/28510115/application-of-decellularized-tissue-scaffolds-in-ovarian-tissue-transplantation
#4
Enes Taylan, Kutluk Oktay
In tissue engineering, decellularized scaffolds have been proved to have remarkable capacity to promote regeneration in various organs such as kidney, heart, lung, and liver. Marrying the field of cryobiology and reproductive medicine resulted in considerable progress and breakthroughs, which led to the emergence of ovarian tissue cryopreservation and transplantation as a promising option for fertility preservation. Here we describe an innovative application of decellularized tissue scaffolds as a regenerative platform for reconstruction of ovarian grafts for auto-transplantation...
May 17, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28463580/tissue-specific-extracellular-matrix-enhances-skeletal-muscle-precursor-cell-expansion-and-differentiation-for-potential-application-in-cell-therapy
#5
Deying Zhang, Yong Zhang, Yuanyuan Zhang, Hualin Yi, Zhan Wang, Rongpei Wu, Dawei He, Guanghui Wei, Shicheng Wei, Yun Hu, Junhong Deng, Tracy Criswell, James J Yoo, Yu Zhou, Anthony Atala
Skeletal muscle precursor cells (MPCs) are considered a key candidate for cell therapy in the treatment of skeletal muscle dysfunction due to injury, disease or age. However, expansion of a sufficient number of functional skeletal muscle cells in vitro from a small tissue biopsy has been challenging due to changes in phenotypic expression of these cells under traditional culture conditions. Thus, the aim of the study was to develop a better culture system for the expansion and myo-differentiation of MPCs that could further be used for therapy...
May 2, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28439901/biomimetic-strategies-to-recapitulate-organ-specific-microenvironments-for-studying-breast-cancer-metastasis
#6
REVIEW
Akshay A Narkhede, Lalita A Shevde, Shreyas S Rao
The progression of breast cancer from the primary tumor setting to the metastatic setting is the critical event defining stage IV disease, no longer considered curable. The microenvironment at specific organ sites is known to play a key role in influencing the ultimate fate of metastatic cells; yet microenvironmental mediated-molecular mechanisms underlying organ specific metastasis in breast cancer are not well understood. This review discusses biomimetic strategies employed to recapitulate metastatic organ microenvironments, particularly, bone, liver, lung and brain to elucidate the mechanisms dictating metastatic breast cancer cell homing and colonization...
April 25, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28437180/a-drug-induced-hybrid-electrospun-poly-capro-lactone-cell-derived-extracellular-matrix-scaffold-for-liver-tissue-engineering
#7
Rhiannon Grant, David C Hay, Anthony Callanan
Liver transplant is the only treatment option for patients with end-stage liver failure, however, there are too few donor livers available for transplant. Whole organ tissue engineering presents a potential solution to the problem of rapidly escalating donor liver shortages worldwide. A major challenge for liver tissue engineers is the creation of a hepatocyte microenvironment; a niche in which liver cells can survive and function optimally. While polymers and decellularized tissues pose an attractive option for scaffold manufacturing, neither alone has thus far proved sufficient...
May 3, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28372287/organ-reconstruction-dream-or-reality-for%C3%A2-the%C3%A2-future
#8
J-F Stoltz, L Zhang, J S Ye, N De Isla
The relevance of research on reconstructed organs is justified by the lack of organs available for transplant and the growing needs for the ageing population. The development of a reconstructed organ involves two parallel complementary steps: de-cellularization of the organ with the need to maintain the structural integrity of the extracellular matrix and vascular network and re-cellularization of the scaffold with stem cells or resident cells.Whole organ engineering for liver, heart, lung or kidneys, is particularly difficult because of the structural complexity of organs and heterogeneity of cells...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28372286/research-progress-in-liver-tissue-engineering
#9
Lei Zhang, Zheng Guan, Jun-Song Ye, Yan-Feng Yin, Jean-François Stoltz, Natalia de Isla
Liver transplantation is the definitive treatment for patients with end-stage liver diseases (ESLD). However, it is hampered by shortage of liver donor. Liver tissue engineering, aiming at fabricating new livers in vitro, provides a potential resolution for donor shortage. Three elements need to be considered in liver tissue engineering: seeding cell resources, scaffolds and bioreactors. Studies have shown potential cell sources as hepatocytes, hepatic cell line, mesenchymal stem cells and others. They need scaffolds with perfect biocompatiblity, suitable micro-structure and appropriate degradation rate, which are essential charateristics for cell attachment, proliferation and secretion in forming extracellular matrix...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28277649/development-of-liver-decellularized-extracellular-matrix-bioink-for-three-dimensional-cell-printing-based-liver-tissue-engineering
#10
Hyungseok Lee, Wonil Han, Hyeonji Kim, Dong-Heon Ha, Jinah Jang, Byoung Soo Kim, Dong-Woo Cho
The liver is an important organ and plays major roles in the human body. Because of the lack of liver donors after liver failure and drug-induced liver injury, much research has focused on developing liver alternatives and liver in vitro models for transplantation and drug screening. Although numerous studies have been conducted, these systems cannot faithfully mimic the complexity of the liver. Recently, three-dimensional (3D) cell printing technology has emerged as one of a number of innovative technologies that may help to overcome this limitation...
March 21, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28162155/-three-dimensional-circulation-perfusion-culture-of-hepatocytes-in-the-liver-decellularizedscaffold
#11
L Wang, P C Zhou, S S Zhu, Y Wang, X J Fan, M Y Zhu, Z W Wang, H X Qian
Objective: The intact rat liver decellularized scaffolds were preparedand repopulated hepatocytes by continuous perfusion technology.Toprovideexperimental support for the application of decellularized liver scaffolds in liver engineering. Method: Decellularized liver scaffolds were obtained by perfusing method. The composition and structure was examined by HE, Masson, Sirius red stain and immunofluorescence. The ultrastructure was examined by scanning electron microscope (SEM). DNA content was used to confirm the effect of decellularization...
January 24, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28127796/construction-of-bioengineered-hepatic-tissue-derived-from-human-umbilical-cord-mesenchymal-stem-cells-via-aggregation-culture-in-porcine-decellularized-liver-scaffolds
#12
Yi Li, Qiong Wu, Yujia Wang, Li Li, Fei Chen, Yujun Shi, Ji Bao, Hong Bu
BACKGROUND: An individualized, tissue-engineered liver suitable for transplanting into a patient with liver disease would be of great benefit to the patient and the healthcare system. The tissue-engineered liver would possess the functions of the original healthy organ. Two fields of study, (i) using decellularized tissue as cell scaffolding, and (ii) stem cell differentiation into functional cells, are coming together to make this concept feasible. The decellularized liver scaffolds (DLS) can interact with cells to promote cell differentiation and signal transduction and three-dimensional (3D) stem cell aggregations can maintain the phenotypes and improve functions of stem cells after differentiation by undergoing cell-cell contact...
January 26, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/28102609/immunogenicity-of-hepatic-differentiated-human-umbilical-cord-mesenchymal-stem-cells-promoted-by-porcine-decellularized-liver-scaffolds
#13
Yi Li, Qiong Wu, Yujia Wang, Li Li, Fei Chen, Yujun Shi, Hong Bu, Ji Bao
Cell-based approaches, including hepatocyte transplantation and tissue-engineered livers, offer promising alternatives and are expected to help support patients with liver diseases until liver transplantation or recovery via regeneration of the damaged liver. However, the success of cell therapies remains dependent on how well the cells are accepted after transplantation and is directly related to their degree of immunogenicity. In this study, hepatic differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) was induced in the traditional monolayer (2D) culture and newly established three-dimensional (3D) aggregation culture with the porcine decellularized liver scaffold (DLS) system (3D-DLS)...
January 19, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/28030865/recent-advances-in-decellularization-and-recellularization-for-tissue-engineered-liver-grafts
#14
REVIEW
Yujia Wang, Clara T Nicolas, Harvey S Chen, Jeffery J Ross, Silvana B De Lorenzo, Scott L Nyberg
Liver transplantation from deceased or living human donors remains the only proven option for patients with end-stage liver disease. However, the shortage of donor organs is a significant clinical concern that has led to the pursuit of tissue-engineered liver grafts generated from decellularized liver extracellular matrix and functional cells. Investigative efforts on optimizing both liver decellularization and recellularization protocols have been made in recent decades. In the current review, we briefly summarize these advances, including the generation of high-quality liver extracellular matrix scaffolds, evaluation criteria for quality control, modification of matrix for enhanced properties, and reseeding strategies...
2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/28029279/recellularization-via-the-bile-duct-supports-functional-allogenic-and-xenogenic-cell-growth-on-a-decellularized-rat-liver-scaffold
#15
Wessam Hassanein, Mehmet C Uluer, John Langford, Jhade D Woodall, Arielle Cimeno, Urmil Dhru, Avraham Werdesheim, Joshua Harrison, Carlos Rivera-Pratt, Stephen Klepfer, Ali Khalifeh, Bryan Buckingham, Philip S Brazio, Dawn Parsell, Charlie Klassen, Cinthia Drachenberg, Rolf N Barth, John C LaMattina
Recent years have seen a proliferation of methods leading to successful organ decellularization. In this experiment we examine the feasibility of a decellularized liver construct to support growth of functional multilineage cells. Bio-chamber systems were used to perfuse adult rat livers with 0.1% SDS for 24 hours yielding decellularized liver scaffolds. Initially, we recellularized liver scaffolds using a human tumor cell line (HepG2, introduced via the bile duct). Subsequent studies were performed using either human tumor cells co-cultured with human umbilical vein endothelial cells (HUVECs, introduced via the portal vein) or rat neonatal cell slurry (introduced via the bile duct)...
January 2, 2017: Organogenesis
https://www.readbyqxmd.com/read/27957815/evolution-of-graft-morphology-and-function-after-recellularization-of-decellularized-rat-livers
#16
Antje Butter, Khalid Aliyev, Karl-Herbert Hillebrandt, Nathanael Raschzok, Martin Kluge, Nicolai Seiffert, Peter Tang, Hendrik Napierala, Muhammad Imtiaz Ashraf, Anja Reutzel-Selke, Andreas Andreou, Johann Pratschke, Igor Maximilian Sauer, Benjamin Struecker
Decellularization of livers is a well-established procedure. Data on different reseeding techniques or the functional evolution and re-organization processes of repopulated grafts remains limited. We established a proprietary, customized bioreactor to repopulate decellularized rat livers (n = 21) with primary rat hepatocytes (150 x 10(6) cells) via the hepatic artery and to subsequently evaluate graft morphology and function during seven days of ex vivo perfusion. Grafts were analyzed at 1 h, 6 h, 12 h, 24 h, 3d, 5d and 7d after recellularization (all n = 3) by immuno-histologic evaluation, hepatocyte-related enzyme (AST, ALT, LDH) and albumin measurement in the perfusate...
December 13, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27862079/discarded-livers-find-a-new-life-engineered-liver-grafts-using-hepatocytes-recovered-from-marginal-livers
#17
Basak E Uygun, Maria-Louisa Izamis, Maria Jaramillo, Yibin Chen, Gavrielle Price, Sinan Ozer, Martin L Yarmush
Treatment for end-stage liver failure is restricted by the critical shortage of donor organs; about 4000 people die in the USA while waiting for a transplantable organ. This situation has been a major driving force behind the rise of tissue engineering to build artificial tissues/organs. Recent advancements in creating transplantable liver grafts using decellularized liver scaffolds bring the field closer to clinical translation. However, a source of readily available and highly functional adult hepatocytes in adequate numbers for regenerative liver therapies still remains unclear...
November 8, 2016: Artificial Organs
https://www.readbyqxmd.com/read/27811394/sympathetic-neurotransmitters-promote-the-process-of-recellularization-in-decellularized-liver-matrix-via-activating-the-il-6-stat3-pathway
#18
Xudong Wen, Hongbo Huan, Xiaojun Wang, Xuejiao Chen, Lili Wu, Yujun Zhang, Weihui Liu, Ping Bie, Feng Xia
Recellularized liver, as an approach for hepatic tissue engineering, is an effective alternative to orthotopic liver transplantation for end-stage hepatic failure. When compared with normal liver, recellularized liver has a disparity in hepatocyte viability and function, owing to the difficulty of fully simulating the microenvironment of liver. Although the sympathetic nervous system (SNS) is considered an important constituent of liver function, few studies have examined the effect of the SNS on hepatic tissue engineering...
November 4, 2016: Biomedical Materials
https://www.readbyqxmd.com/read/27805597/study-of-viral-vectors-in-a-three-dimensional-liver-model-repopulated-with-the-human-hepatocellular-carcinoma-cell-line-hepg2
#19
Thomas Hiller, Viola Röhrs, Eva-Maria Dehne, Anke Wagner, Henry Fechner, Roland Lauster, Jens Kurreck
This protocol describes the generation of a three-dimensional (3D) ex vivo liver model and its application to the study and development of viral vector systems. The model is obtained by repopulating the extracellular matrix of a decellularized rat liver with a human hepatocyte cell line. The model permits studies in a vascularized 3D cell system, replacing potentially harmful experiments with living animals. Another advantage is the humanized nature of the model, which is closer to human physiology than animal models...
October 24, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27741254/fibronectin-extra-domain-a-promotes-liver-sinusoid-repair-following-hepatectomy
#20
Bridget Sackey-Aboagye, Abby L Olsen, Sarmistha M Mukherjee, Alexander Ventriglia, Yasuyuki Yokosaki, Linda E Greenbaum, Gi Yun Lee, Hani Naga, Rebecca G Wells
Liver sinusoidal endothelial cells (LSECs) are the main endothelial cells in the liver and are important for maintaining liver homeostasis as well as responding to injury. LSECs express cellular fibronectin containing the alternatively spliced extra domain A (EIIIA-cFN) and increase expression of this isoform after liver injury, although its function is not well understood. Here, we examined the role of EIIIA-cFN in liver regeneration following partial hepatectomy. We carried out two-thirds partial hepatectomies in mice lacking EIIIA-cFN and in their wild type littermates, studied liver endothelial cell adhesion on decellularized, EIIIA-cFN-containing matrices and investigated the role of cellular fibronectins in liver endothelial cell tubulogenesis...
2016: PloS One
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