keyword
https://read.qxmd.com/read/38526339/fluorescent-nanodiamond-immunosensors-for-clinical-diagnostics-of-tuberculosis
#1
JOURNAL ARTICLE
Trong-Nghia Le, Mhikee Janella N Descanzo, Wesley W-W Hsiao, Po-Chi Soo, Wen-Ping Peng, Huan-Cheng Chang
Fluorescent nanodiamonds (FNDs) are carbon nanoparticles containing a dense ensemble of nitrogen-vacancy defects as color centers. These centers have exceptional photostability and unique quantum properties, making them useful for ultrasensitive biosensing applications. This work employed FNDs conjugated with antibodies as magneto-optical immunosensors for tuberculosis (TB) diagnostics using competitive spin-enhanced lateral flow immunoassay (SELFIA). ESAT6 (6-kDa early secretory antigenic target) of Mycobacterium tuberculosis is a clinical marker of TB...
March 25, 2024: Journal of Materials Chemistry. B, Materials for Biology and Medicine
https://read.qxmd.com/read/38520322/novel-ultrasensitive-immunoassay-for-the-selective-quantification-of-tau-oligomers-and-related-soluble-aggregates
#2
JOURNAL ARTICLE
Tohidul Islam, Hlin Kvartsberg, Anuradha Sehrawat, Przemysław R Kac, Bruno Becker, Maria Olsson, Eric E Abrahamson, Henrik Zetterberg, Milos D Ikonomovic, Kaj Blennow, Thomas K Karikari
INTRODUCTION: Tau aggregation into paired helical filaments and neurofibrillary tangles is characteristic of Alzheimer's disease (AD) and related disorders. However, biochemical assays for the quantification of soluble, earlier-stage tau aggregates are lacking. We describe an immunoassay that is selective for tau oligomers and related soluble aggregates over monomers. METHODS: A homogeneous (single-antibody) immunoassay was developed using a novel anti-tau monoclonal antibody and validated with recombinant and brain tissue-derived tau...
March 23, 2024: Alzheimer's & Dementia: the Journal of the Alzheimer's Association
https://read.qxmd.com/read/38499431/ultrasensitive-detection-of-zearalenone-based-on-electrochemiluminescent-immunoassay-with-zr-mof-nanoplates-and-au-mos-2-nanoflowers
#3
JOURNAL ARTICLE
Lu Peng, Xinyue Qian, Ya Jin, Xiangyang Miao, Anping Deng, Jianguo Li
In this work, an effective competitive-type electrochemiluminescence (ECL) immunosensor was constructed for zearalenone determination by using Zr-MOF nanoplates as the ECL luminophore and Au@MoS2 nanoflowers as the substrate material. Zr-MOF have an ultra-thin sheet-like structure that accelerates the transfer of electrons, ions and co-reactant intermediates, which exhibited strong and stable anodic luminescence. The three-dimensional Au@MoS2 nanoflowers would form a thin film modification layer on the glassy carbon electrode (GCE)...
April 22, 2024: Analytica Chimica Acta
https://read.qxmd.com/read/38495999/a-prototype-of-ultrasensitive-time-resolved-fluoroimmunoassay-for-the-quantitation-of-lead-in-plasma-using-a-fluorescence-enhanced-europium-chelate-label-for-the-detection-system
#4
JOURNAL ARTICLE
Ibrahim A Darwish, Kenzi Suzuki, Hiroshi Ogawa, Zongzhi Wang
This study describes the prototype of a novel ultra-sensitive time-resolved fluoroimmunoassay (TRFIA) for the quantification of lead (Pb) in plasma. The assay procedures were conducted in 96-microwell plates and involved the competitive binding format. The assay used a mouse monoclonal antibody, designated as 2C33, that specifically recognized the diethylenetriamine pentaacetic acid chelate of Pb (Pb-DTPA) but did not recognize Pb-free DTPA chelator. The antigen used for coating onto the inner surfaces of assay plate microwells was Pb-DTPA conjugated with bovine serum albumin protein (Pb-DTPA-BSA)...
March 14, 2024: RSC Advances
https://read.qxmd.com/read/38491108/novel-electrochemical-platform-based-on-c-3-n-4-graphene-composite-for-the-detection-of-neuron-specific-enolase-as-a-biomarker-for-lung-cancer
#5
JOURNAL ARTICLE
Zhang Junping, Wei Zheng, Tang ZhengFang, L I Ji Yue, An PengHang, Zhang Mingli, An Hongzhi
Lung cancer remains the leading cause of cancer mortality worldwide. Small cell lung cancer (SCLC) accounts for 10-15% of cases and has an overall 5-years survival rate of only 15%. Neuron-specific enolase (NSE) has been identified as a useful biomarker for early SCLC diagnosis and therapeutic monitoring. This work reports an electrochemical immunosensing platform based on a graphene-graphitic carbon nitride (g-C3 N4 ) nanocomposite for ultrasensitive NSE detection. The g-C3 N4 nanosheets and graphene nanosheets were synthesized via liquid exfoliation and integrated through self-assembly to form the nanocomposite...
March 16, 2024: Scientific Reports
https://read.qxmd.com/read/38490023/ultrasensitive-protein-and-exosome-analysis-based-on-a-rolling-circle-amplification-assisted-crispr-cas12a-strategy
#6
JOURNAL ARTICLE
Jingjing Shi, Chao Lei, Wenjiao Fan, Yuanyuan Sun, Chenghui Liu
CRISPR/Cas12a system has attracted extensive concern in biosensing due to its high specificity and programmability. Nevertheless, existing Cas12a-based assays mainly focus on nucleic acid detection and have limitations in non-nucleic acid biomarker analysis. To broaden the application prospect of the CRISPR/Cas technology, a cascade Cas12a biosensing platform is reported by combining dual-functionalized gold nanoparticles (FGNPs)-assisted rolling circle amplification (RCA) and Cas12a trans-cleavage activity (GAR-Cas) for ultrasensitive protein and exosome analysis...
March 11, 2024: Talanta
https://read.qxmd.com/read/38471066/dual-modal-colorimetric-and-surface-enhanced-raman-scattering-sers-based-lateral-flow-immunoassay-for-ultrasensitive-detection-of-sars-cov-2-using-a-plasmonic-gold-nanocrown
#7
JOURNAL ARTICLE
Supriya Atta, Yuanhao Zhao, Joy Qiaoyi Li, Tuan Vo-Dinh
The 2019 coronavirus disease (COVID-19) outbreak created an unprecedented need for rapid, sensitive, and cost-effective point-of-care diagnostic tests to prevent and mitigate the spread of the SARS-CoV-2 virus. Herein, we demonstrated an advanced lateral flow immunoassay (LFIA) platform with dual-functional [colorimetric and surface-enhanced Raman scattering (SERS)] detection of the spike 1 (S1) protein of SARS-CoV-2. The nanosensor was integrated with a specially designed core-gap-shell morphology consisting of a gold shell decorated with external nanospheres, a structure referred to as gold nanocrown (GNC), labeled with a Raman reporter molecule 1,3,3,1',3',3'-hexamethyl-2,2'-indotricarbocyanine iodide (HITC) to produce a strong colorimetric signal as well as an enhanced SERS signal...
March 12, 2024: Analytical Chemistry
https://read.qxmd.com/read/38469186/a-novel-ultrasensitive-chemiluminescence-enzyme-immunoassay-by-employment-of-a-signal-enhancement-of-horseradish-peroxidase-luminol-hydrogen-peroxide-reaction-for-the-quantitation-of-atezolizumab-a-monoclonal-antibody-used-for-cancer-immunotherapy
#8
JOURNAL ARTICLE
Ibrahim A Darwish, Mohammad A H Ali, Mohammed S Alsalhi, Daohong Zhang
This study describes, for the first time, the development and validation of a novel ultrasensitive chemiluminescence enzyme immunoassay (CLEIA) for the quantification of atezolizumab (ATZ), a monoclonal antibody approved by the FDA for treatment of different types of cancer. The assay involved the non-competitive binding of ATZ to its specific antigen (PD-L1 protein). The immune complex of PD-L1/ATZ formed on the internal surface of the plate wells was quantified by a novel chemiluminescence (CL)-producing horseradish peroxidase (HRP) reaction...
March 6, 2024: RSC Advances
https://read.qxmd.com/read/38462975/click-reaction-mediated-fluorescent-immunosensor-based-on-cu-mof-nanoparticles-for-ultrasensitive-and-high-throughput-detection-of-aflatoxin-b-1-in-food-samples
#9
JOURNAL ARTICLE
Feng Hong, Yongkun Zhao, Shixing Pan, Liangqiong Ren, Feng Jiang, Long Wu, Yiping Chen
Due to the high toxicity of aflatoxin B1 and its risks to human health, we developed a click reaction-mediated automated fluorescent immunosensor (CAFI) for sensitive detection of aflatoxin B1 based on the Cu(I)-catalyzed click reaction. With its large specific surface area, a copper-based metal-organic framework (Cu-MOF) was synthesized to adsorb and enrich the copper ion (Cu(II)) and then load the complete antigen (BSA-AFB1 ). After the immunoreaction, Cu(II) inside the Cu-MOF-Antigen conjugate would be reduced to Cu(I) in the presence of sodium ascorbate, which triggered the click reaction between the fluorescent donor-modified DNA and the receptor-modified complementary DNA to lead to a fluorescence signal readout...
March 11, 2024: Journal of Agricultural and Food Chemistry
https://read.qxmd.com/read/38436786/development-of-a-flow-injection-chemiluminescence-immunoassay-based-on-des-mediated-cuco-2-o-4-nanoenzyme-for-ultrasensitive-detection-of-zearalenone-in-foods
#10
JOURNAL ARTICLE
Xinziwei Zeng, Jiao Li, Lingyun Xu, Anping Deng, Jianguo Li
Nanoenzymes have been widely used to construct biosensors because of their cost-effectiveness, high stability, and easy modification. At the same time, the discovery of deep eutectic solvents (DES) was a great breakthrough in green chemistry, and their combination with different materials can improve the sensing performance of biosensors. In this work, we report an immunosensor using CuCo2 O4 nanoenzyme combined with flow injection chemiluminescence immunoassay for the automated detection of zearalenone (ZEN)...
March 4, 2024: Mikrochimica Acta
https://read.qxmd.com/read/38415592/-tecoma-stans-floral-extract-based-biosynthesis-for-enhanced-surface-plasmon-coupled-emission-and-a-preliminary-study-on-fluoroimmunoassay
#11
JOURNAL ARTICLE
Sriram Rathnakumar, Seemesh Bhaskar, Venketesh Sivaramakrishnan, Naga Sai Visweswar Kambhampati, Venkatesh Srinivasan, Sai Sathish Ramamurthy
We demonstrate the synthesis of biogenic supported silver spiked star architectures and their application to increase the electromagnetic field intensity at its tips that enhance plasmon-coupled emission. Tecoma stans floral extract has been used to synthesize silver nanocubes and spiked stars. We observe ∼445-fold and ∼680-fold enhancements in spacer and cavity configurations, respectively, in the SPCE platform. The hotspot intensity and Purcell factor are evaluated by carrying out finite-difference time-domain (FDTD) simulations...
February 28, 2024: Analytical Chemistry
https://read.qxmd.com/read/38382271/dnazyme-based-ultrasensitive-immunoassay-recent-advances-and-emerging-trends
#12
REVIEW
Meng Wang, Zhe Liu, Chang Liu, Wanghong He, Dui Qin, Minli You
Immunoassay, as the most commonly used method for protein detection, is simple to operate and highly specific. Sensitivity improvement is always the thrust of immunoassays, especially for the detection of trace quantities. The emergence of artificial enzyme, i.e., DNAzyme, provides a novel approach to improve the detection sensitivity of immunoassay. Simultaneously, its advantages of simple synthesis and high stability enable low cost, broad applicability and long shelf life for immunoassay. In this review, we summarized the recent advances in DNAzyme-based immunoassay...
February 9, 2024: Biosensors & Bioelectronics
https://read.qxmd.com/read/38372378/palladium-nanoballs-coupled-with-smartphone-thermal-reader-for-photothermal-lateral-flow-immunoassay-of-a%C3%AE-1-40
#13
JOURNAL ARTICLE
Xiaoli Cai, Yangxing Luo, Yang Song
Amyloid beta 1-40 (Aβ 1-40) is one of the most abundant substances in the body with the capacity to form insoluble aggregates and is a universal biomarker for the prediction of Alzheimer's disease. Here, a palladium nanoball (PNB)-strip was developed and coupled with a smartphone-thermal reader as an ultrasensitive and cost-effective platform for Aβ 1-40 detection. In this study, PNB was synthesized and introduced into lateral flow strips as an alternative signal source to gold nanoparticles to improve sensitivity because the PNB has a better heat generation ability...
February 19, 2024: Journal of Materials Chemistry. B, Materials for Biology and Medicine
https://read.qxmd.com/read/38371773/colorimetric-and-reverse-fluorescence-dual-signal-readout-immunochromatographic-assay-for-the-sensitive-determination-of-sibutramine
#14
JOURNAL ARTICLE
Yun Gui, Yun Zhao, Pengyan Liu, Yulong Wang, Xinxin Mao, Chifang Peng, Bruce D Hammock, Cunzheng Zhang
Later flow immunochromatographic assay has been widely used in clinical, environmental, and other diagnostic applications owing to its high sensitivity and throughput. However, most immunoassays operate in the "turn-off" mode for detecting targets of low molecular weight. The signal intensity decreases as the analyte concentration increases, which poses a challenge for achieving ultrasensitive detection at low concentrations and is counterintuitive to new users. In this work, a fluorometric immunochromatographic assay (FICA) is developed to simultaneously read "turn-on" fluorescent and "turn-off" colorimetric signals, where ZnCdSe/ZnS quantum dots act as fluorescence donors and gold nanoparticles (AuNPs) act as quenchers...
February 13, 2024: ACS Omega
https://read.qxmd.com/read/38367555/cdte-based-water-soluble-fluorescent-probe-for-rapid-detection-of-zilpaterol-in-swine-urine-and-pork
#15
JOURNAL ARTICLE
Yihua Wei, Weihong Li, Yan Han, Yan Xiong, Yuanying Kuang, Jinyan Zhang
Zilpaterol hydrochloride (zilpaterol) is used in animal feed as it can increase the lean meat mass. However, consuming zilpaterol-containing animal products may damage human health. Therefore, rapid detection of zilpaterol is attracting increasing research attention. This study aimed to developed a fast, accurate, and ultrasensitive fluorescence immunoassay based on CdTe quantum dots (QDs). A CdTe QD fluorescence sensor was synthesized from thioglycolic acid using a simple hydrothermal method. The morphology and structure of the CdTe QDs were characterized using transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, and Fourier-transform infrared spectroscopy...
February 5, 2024: Food Chemistry
https://read.qxmd.com/read/38364565/ultrasensitive-fluorescence-microrna-biosensor-by-coupling-hybridization-initiated-exonuclease-i-protection-and-tyramine-signal-amplification
#16
JOURNAL ARTICLE
Fenghui Qi, Yong Ju, Yanian Xiong, Jianzhong Lu, Yuhao Zhang
Tyramine signal amplification (TSA) has made its mark in immunoassay due to its excellent signal amplification ability and short reaction time, but its application in nucleic acid detection is still very limited. Herein, an ultrasensitive microRNA (miRNA) biosensor by coupling hybridization-initiated exonuclease I (Exo I) protection and TSA strategy was established. Target miRNA is complementarily hybridized to the biotin-modified DNA probe to form a double strand, which protects the DNA probe from Exo I hydrolysis...
February 9, 2024: Talanta
https://read.qxmd.com/read/38358834/a-facile-strategy-for-multiplex-protein-detection-by-a-fluorescent-microsphere-based-digital-immunoassay
#17
JOURNAL ARTICLE
Feng Gong, Zhiyou Tan, Xiaoyun Shan, Yixia Yang, Songbai Tian, Fuxiang Zhou, Xinghu Ji, Zhike He
The digital immunoassay is a highly sensitive detection technique based on single-molecule counting and is widely used in the ultrasensitive detection of biomarkers. Herein, we developed a fluorescent microsphere-based digital immunoassay (FMDIA) by employing fluorescent microspheres as both the carriers for immunoreaction and fluorescent reports for imaging. In this approach, the target protein in the sample was captured by fluorescent microspheres to form a biotin-labeled sandwich immunocomplex, and then, the fluorescent microspheres containing the target protein molecules were captured by adding streptavidin-coated magnetic beads (SA-MBs)...
February 15, 2024: Analytical Chemistry
https://read.qxmd.com/read/38357784/dual-readout-ultrasensitive-lateral-flow-immunosensing-of-salmonella-typhimurium-in-dairy-products-by-doping-engineering-powered-nanoheterostructure-with-enhanced-photothermal-performance
#18
JOURNAL ARTICLE
Longhua Shi, Ziqi Wang, Yuechun Li, Jiamin Wang, Jinrui Shan, Junchen Zhuo, Xuechi Yin, Jing Sun, Daohong Zhang, Jianlong Wang
The photothermal lateral flow immunoassay (LFIA) is of great significance to suitable for on-site semiquantitative detection, which has the upper hand in further constructing detection methods for low-concentration targets. Herein, we presented a doping engineering-powered nanoheterostructure with an enhanced photothermal performance strategy, employing bimetallic nanocuboid Pt3 Sn (PSNCs) as a proof of concept. With the help of finite element simulation analysis, the contrast of direct temperature experiment, and the evaluation of photothermal conversion efficiency (η), the distinguished and enthusiastic photothermal feedback of PSNCs is proved...
February 15, 2024: Journal of Agricultural and Food Chemistry
https://read.qxmd.com/read/38335519/sub-second-electrochemiluminescence-imaging-assay-of-sars-cov-2-nucleocapsid-protein-based-on-reticulation-of-endo-coreactants
#19
JOURNAL ARTICLE
Yuansheng Li, Ying Wan, Xuanyu Fu, Jialiang Chen, Weihan Wu, Xuyu Feng, Tiantian Man, Yaqi Huang, Yuhao Piao, Longyi Zhu, Jianping Lei, Shengyuan Deng
The nonphotodriven electrochemiluminescence (ECL) imageology necessitates concentrated coreacting additives plus longtime exposures. Seeking biosafe and streamlined ensembles can help lower the bar for quality ECL bioimaging to which call the crystallized endo-coreaction in nanoreticula might provide a potent solution. Herein, an exo-coreactant-free ECL visualizer was fabricated out in one-pot, which densified the dyad triethylamine analogue: 1,4-diazabicyclo-[2.2.2]octane (DABCO) in the lamellar hive of 9,10-di( p -carboxyphenyl)anthracene (DPA)-Zn2+ ...
February 9, 2024: Analytical Chemistry
https://read.qxmd.com/read/38335120/addressing-signal-drift-and-screening-for-detection-of-biomarkers-with-carbon-nanotube-transistors
#20
JOURNAL ARTICLE
Faris M Albarghouthi, Daria Semeniak, Iman Khanani, James L Doherty, Brittany N Smith, Matthew Salfity, Quentin MacFarlane, Aneesh Karappur, Steven G Noyce, Nicholas X Williams, Daniel Y Joh, Joseph B Andrews, Ashutosh Chilkoti, Aaron D Franklin
Electrical biosensors, including transistor-based devices (i.e., BioFETs), have the potential to offer versatile biomarker detection in a simple, low-cost, scalable, and point-of-care manner. Semiconducting carbon nanotubes (CNTs) are among the most explored nanomaterial candidates for BioFETs due to their high electrical sensitivity and compatibility with diverse fabrication approaches. However, when operating in solutions at biologically relevant ionic strengths, CNT-based BioFETs suffer from debilitating levels of signal drift and charge screening, which are often unaccounted for or sidestepped (but not addressed) by testing in diluted solutions...
February 9, 2024: ACS Nano
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