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Ultrasensitive immunoassay

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https://www.readbyqxmd.com/read/28931875/ultrasensitive-nano-rt-ipcr-for-determination-of-polybrominated-diphenyl-ethers-in-natural-samples
#1
Xiaohan Zhang, Xianyin Ping, Huisheng Zhuang
Extensive polybrominated diphenyl ethers (PBDEs) use has resulted in its increasingly widespread presence in the environment. PBDEs release from existing products can still persist and accumulate in the environment as well as in human and wildlife magnifying through the food web. Due to its ultra-trace amount in the environment, a novel ultrasensitive nano-rt-iPCR assay has been developed to determine polybrominated diphenyl ethers in natural samples. Numerous amino-DNA and polyclonal antibody (anti-PBDE) were immobilized onto the single-walled carbon nanotubes (SWCNTs) to form antibody-SWCNTs-DNA signal amplifier used in the proposed immunoassay system...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28877338/a-switchable-linker-based-immunoassay-for-ultrasensitive-visible-detection-of-salmonella-in-tomatoes
#2
Jungwoo Hahn, Eunghee Kim, Young Sang You, Sundaram Gunasekaran, Seokwon Lim, Young Jin Choi
On-site detection for sensitive identification of foodborne pathogens on fresh produce with minimal use of specialized instrumentation is crucial to the food industry. A switchable linker (SL)-based immunoassay was designed for ultrasensitive on-site detection of Salmonella in tomato samples. The assay is based on large-scale aggregation of gold nanoparticles (GNPs), induced by a quantitative relationship among the biotinylated Salmonella polyclonal antibody (b-Ab) used as the SL, the functionalized GNPs, and Salmonella...
September 6, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28867489/optomechanical-devices-for-deep-plasma-cancer-proteomics
#3
REVIEW
Priscila M Kosaka, Montserrat Calleja, Javier Tamayo
Most of the cancer deaths could be avoided by early detection of the tumor when it is confined to its primary site and it has not metastasized. To this aim, one of the most promising strategies is the discovery and detection of protein biomarkers shed by the young tumor to the bloodstream. Proteomic technologies, mainly mass spectrometry and multiplexed immunoassays, have rapidly developed during last years with improved limits of detection and multiplexing capability. Unfortunately, these developments together major investments and large international efforts have not resulted into new useful protein biomarkers...
August 31, 2017: Seminars in Cancer Biology
https://www.readbyqxmd.com/read/28866979/quantification-of-plasma-phosphorylated-tau-to-use-as-a-biomarker-for-brain-alzheimer-pathology-pilot-case-control-studies-including-patients-with-alzheimer-s-disease-and-down-syndrome
#4
Harutsugu Tatebe, Takashi Kasai, Takuma Ohmichi, Yusuke Kishi, Tomoshi Kakeya, Masaaki Waragai, Masaki Kondo, David Allsop, Takahiko Tokuda
BACKGROUND: There is still a substantial unmet need for less invasive and lower-cost blood-based biomarkers to detect brain Alzheimer's disease (AD) pathology. This study is aimed to determine whether quantification of plasma tau phosphorylated at threonine 181 (p-tau181) is informative in the diagnosis of AD. METHODS: We have developed a novel ultrasensitive immunoassay to quantify plasma p-tau181, and measured the levels of plasma p-tau181 in three cohorts. RESULTS: In the first cohort composed of 20 AD patients and 15 age-matched controls, the plasma levels of p-tau181 were significantly higher in the AD patients than those in the controls (0...
September 4, 2017: Molecular Neurodegeneration
https://www.readbyqxmd.com/read/28852782/ultrasensitive-sers-immunoassay-based-on-diatom-biosilica-for-detection-of-interleukins-in-blood-plasma
#5
Agnieszka Kamińska, Myroslav Sprynskyy, Katarzyna Winkler, Tomasz Szymborski
An ultrasensitive surface-enhanced Raman scattering (SERS) immunoassay based on diatom biosilica with integrated gold nanoparticles (AuNPs) for the detection of interleukin 8 (IL-8) in blood plasma has been developed. The SERS sensing originates from unique features of the diatom frustules, which are capable of enhancing the localized surface-plasmon resonance of metal nanostructures. The SERS immune tags ware fabricated by functionalizing 70-nm Au nanoparticles with DTNB (i.e., 5,5'-dithiobis(2-nitrobenzoic acid)), which acted as a Raman reporter molecule, as well as the specific antibodies...
August 29, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28800507/ultrasensitive-electrochemiluminescence-immunoassay-for-simultaneous-determination-of-ca125-and-ca15-3-tumor-markers-based-on-pamam-sulfanilic-acid-ru-bpy-3-2-and-pamam-cdte-cds-nanocomposite
#6
Bahareh Babamiri, Rahman Hallaj, Abdollah Salimi
A multiplex ultrasensitive electrochemiluminescence (ECL) immunoassay was developed for the simultaneous determination of two different tumor markers, cancer antigen 153 (CA 15-3) and cancer antigen 125 (CA 125) using polyamidoamine dendrimer-quantum dots (PAMAM-QDs) and PAMAM-sulfanilic acid-Ru(bpy)3(2+) as the signal probes and Fe3O4-SiO2 as a magnetic bead. The CdTe@CdS- QDs and Ru(bpy)3(2+) at the presence of tripropyl amine (TPA) as coreactant generate ECL at an applied voltage of + 1.2V (vs Ag/AgCl) in two different wavelengths 500 and 620nm, respectively...
July 28, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28734353/streptavidin-biotin-peroxidase-nanocomplex-amplified-microfluidics-immunoassays-for-simultaneous-detection-of-inflammatory-biomarkers
#7
Jing Wu, Yiping Chen, Mingzhu Yang, Yu Wang, Cheng Zhang, Mo Yang, Jiashu Sun, Mengxia Xie, Xingyu Jiang
Simultaneous, sensitive and quantitative detection of biomarkers in infectious disease is crucial for guiding antimicrobial treatment and predicting prognosis. This work reported an ultrasensitive and quantitative microfluidic immunoassay combined with the streptavidin-biotin-peroxidase (SA-B-HRP) nanocomplex-signal amplification system (MIS) to detect two inflammatory biomarkers, procalcitonin (PCT, for discriminating bacterial infections from nonbacterial infections) and interleukin-6 (IL-6, for monitoring the kinetics of infectious disease) simultaneously...
August 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28724286/deep-dive-targeted-quantification-for-ultrasensitive-analysis-of-proteins-in-nondepleted-human-blood-plasma-serum-and-tissues
#8
Song Nie, Tujin Shi, Thomas L Fillmore, Athena A Schepmoes, Heather Brewer, Yuqian Gao, Ehwang Song, Hui Wang, Karin D Rodland, Wei-Jun Qian, Richard D Smith, Tao Liu
Mass spectrometry-based targeted proteomics (e.g., selected reaction monitoring, SRM) is emerging as an attractive alternative to immunoassays for protein quantification. Recently we have made significant progress in SRM sensitivity for enabling quantification of low nanograms per milliliter to sub-naograms per milliliter level proteins in nondepleted human blood plasma/serum without affinity enrichment. However, precise quantification of extremely low abundance proteins (e.g., ≤ 100 pg/mL in blood plasma/serum) using targeted proteomics approaches still remains challenging, especially for these samples without available antibodies for enrichment...
September 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28723149/cyclewise-operation-of-printed-mos2-transistor-biosensors-for-rapid-biomolecule-quantification-at-femtomolar-levels
#9
Byunghoon Ryu, Hongsuk Nam, Bo-Ram Oh, Yujing Song, Pengyu Chen, Younggeun Park, Wenjie Wan, Katsuo Kurabayashi, Xiaogan Liang
Field-effect transistors made from MoS2 and other emerging layered semiconductors have been demonstrated to be able to serve as ultrasensitive biosensors. However, such nanoelectronic sensors still suffer seriously from a series of challenges associated with the poor compatibility between electronic structures and liquid analytes. These challenges hinder the practical biosensing applications that demand rapid, low-noise, highly specific biomolecule quantification at femtomolar levels. To address such challenges, we study a cyclewise process for operating MoS2 transistor biosensors, in which a series of reagent fluids are delivered to the sensor in a time-sequenced manner and periodically set the sensor into four assay-cycle stages, including incubation, flushing, drying, and electrical measurement...
February 24, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28662470/multiple-functional-strategies-for-amplifying-sensitivity-of-amperometric-immunoassay-for-tumor-markers-a-review
#10
REVIEW
Zhongxue Tang, Zhanfang Ma
Multiple functional strategies have shown great potential in ultrasensitive amperometric immunoassays for tumor markers, which promote conductivity and signal multiple amplification. The sensitivity of amperometric immunoassays is significantly affected by the conductivity and specific area of the sensing interface as well as the electrochemical activity of redox species. Thus, these strategies are generally based on integrating various materials together and endowing immunosensing systems with many advantages, such as large specific area, high electrochemical activity, good conductivity, biocompatibility, and catalytic performance...
June 21, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28650005/sub-picomolar-label-free-detection-of-thrombin-using-electrochemical-impedance-spectroscopy-of-aptamer-functionalized-mos2
#11
Kai-Chun Lin, Badrinath Jagannath, Sriram Muthukumar, Shalini Prasad
An ultrasensitive aptasensor for the label free non-faradaic detection of thrombin has been demonstrated on molybdenum disulphide (MoS2) nanosheets. These nanosheets were physiochemically immobilized onto a silicon micro-electrode platform. Thrombin detection was achieved through the charge modulation of the electrical double layer due to the specific and dose dependent binding of thrombin to the surface of thiol terminated ssDNA aptamer functionalized MoS2 nanosheets. Electrical double layer charge modulation associated with thrombin binding was characterized using electrochemical impedance spectroscopy...
July 24, 2017: Analyst
https://www.readbyqxmd.com/read/28627218/performance-evaluation-of-three-platforms-with-ultrasensitive-ligand-binding-assay-potential
#12
Kyra Juliana Cowan, Albert Geiger, Hans Hornauer, Robyn Rourick, Patricia Yanira Siguenza
AIM: We evaluated three immunoassay-based technologies and their biomarker kits, by determining precision, parallelism and detectability of analytes of interest. MATERIALS & METHODS: We compared ultrasensitive assays for three biomarkers: interleukins IL-6, IL-13 and IL-17A using kits obtained from Roche (IMPACT platform - proprietary platform), Singulex (Erenna(®)) and Quanterix (Simoa(™)). We defined the true LLOD as the LLOQs, and provided disease-specific parallelism results and detectability levels for endogenous analyte, which were good across platforms, though they varied from analyte to analyte...
June 2017: Bioanalysis
https://www.readbyqxmd.com/read/28550286/ultrasensitive-multiplexed-immunoassay-of-autophagic-biomarkers-based-on-au-rgo-and-au-nanocages-amplifying-electrochemcial-signal
#13
Guannan Wang, Yankun Li, Jinlei Liu, Yajing Yuan, Zhaoliang Shen, Xifan Mei
A novel sandwich-assay electrochemical immunosensor for simultaneous determination of autophagic biomarkers was introduced for the first time, the gold-reduced grapheme oxide nanocomposite (Au/r-GO) set as a good conductive platform with super high specific area, and provided more binding sites for the both antibodies of Beclin-1 and LC3B-II. While Au nanocages (AuNCs) served as good conductive platform to encapsulate a large amount of redox probe and secondary antibodies for signal amplification, due to the abundant reactive oxygen functional groups on its surface...
May 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28505563/ultrasensitive-magnetic-field-assisted-surface-plasmon-resonance-immunoassay-for-human-cardiac-troponin-i
#14
Qiong Wu, Ying Sun, Di Zhang, Shuo Li, Yue Zhang, Pinyi Ma, Yang Yu, Xinghua Wang, Daqian Song
An ultrasensitive surface plasmon resonance (SPR) immunoassay was developed for the specific detection of human cardiac troponin I (cTnI), a principle diagnostic marker for myocardial damage. The thin gold film evaporated on a glass slate, which was employed as the SPR sensing film, was modified by hollow gold nanoparticles (HGNPs) and polydopamine (PDA) sequentially, and then was immobilized with antibodies for specific recognition of target analyte. Electronic coupling of the surface plasmon waves originating from the HGNPs and the gold film leads to the remarkable amplification of SPR response...
May 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28495100/ultrasensitive-direct-competitive-flisa-using-highly-luminescent-quantum-dot-beads-for-tuning-affinity-of-competing-antigens-to-antibodies
#15
Sicheng Xiong, Yaofeng Zhou, Xiaolin Huang, Ruijin Yu, Weihua Lai, Yonghua Xiong
Herein, for the first time we report a novel direct competitive fluorescence-linked immunosorbent assay (dcFLISA) for the ultrasensitive detection of ochratoxin A (OTA) by introducing a large size polymer beads loaded with quantum dots (QBs) as carrier of competing antigen for decreasing binding affinity to antibody and enhancing the fluorescent signal intensity. When using 255 nm QBs as carrier of competing antigen, the equilibrium dissociation constant of QB based competing antigen to antibodies can be tuned to 100 times higher than that of the horseradish peroxidase (HRP) based competing antigen by controlling labeled amounts of antigen on the surface of QBs...
June 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28488883/ultrasensitive-bioanalysis-current-status-and-future-trends
#16
Stéphanie Simon, Eric Ezan
Ligand-binding techniques such as immunoassays, the reference for clinical diagnosis, offer a wide range of innovative approaches based on signal DNA amplification, nanotechnologies or digital assays, which result in technologies with sensitivities more than 1000-times that of formats used 20 years ago. Providing that these technologies gain acceptance and translate into robust commercial platforms, we expect that several fields will be impacted in the near future, including the clinical diagnosis of cancer markers, the early detection of infectious diseases and the safety of biotherapeutics...
May 2017: Bioanalysis
https://www.readbyqxmd.com/read/28483527/immuno-pcr-with-digital-readout
#17
Hendrik Schröder, Maximilian Grösche, Michael Adler, Mark Spengler, Christof M Niemeyer
Immuno-PCR (IPCR) combines the versatile ELISA antigen detection with ultrasensitive PCR signal amplification, thereby enabling the highly sensitive detection of a broad range of targets with a typically very large dynamic detection range. The quantification of the antigen is usually achieved by real-time PCR, which provides a correlation between the target concentration and amplified DNA marker. We here report on the implementation of digital droplet PCR as a means for direct quantification of DNA copies to enable the highly sensitive detection of protein biomarkers...
June 24, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28467848/automated-microfluidic-droplet-based-sample-chopper-for-detection-of-small-fluorescence-differences-using-lock-in-analysis
#18
Jean T Negou, L Adriana Avila, Xiangpeng Li, Tesfagebriel M Hagos, Christopher J Easley
Fluorescence is widely used for small-volume analysis and is a primary tool for on-chip detection in microfluidic devices, yet additional expertise, more elaborate optics, and phase-locked detectors are needed for ultrasensitive measurements. Recently, we designed a microfluidic analog to an optical beam chopper (μChopper) that alternated formation of picoliter volume sample and reference droplets. Without complex optics, the device negated large signal drifts (1/f noise), allowing absorbance detection in a mere 27 μm optical path...
June 6, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28460522/nanobody-based-immunoassay-for-human-soluble-epoxide-hydrolase-detection-using-polymeric-horseradish-peroxidase-polyhrp-for-signal-enhancement-the-rediscovery-of-polyhrp
#19
Dongyang Li, Yongliang Cui, Christophe Morisseau, Shirley J Gee, Candace S Bever, Xiangjiang Liu, Jian Wu, Bruce D Hammock, Yibin Ying
Soluble epoxide hydrolase (sEH) is a potential pharmacological target for treating hypertension, vascular inflammation, cancer, pain, and multiple cardiovascular related diseases. A variable domain of the heavy chain antibody (termed single domain antibody (sdAb), nanobody, or VHH) possesses the advantages of small size, high stability, ease of genetic manipulation, and ability for continuous manufacture, making such nanobody a superior choice as an immunoreagent. In this work, we developed an ultrasensitive nanobody based immunoassay for human sEH detection using polymeric horseradish peroxidase (PolyHRP) for signal enhancement...
June 6, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28451145/competitive-method-based-electrochemiluminescent-assay-with-protein-nucleotide-conversion-for-ratio-detection-to-efficiently-monitor-the-drug-resistance-of-cancer-cells
#20
Wen-Bin Liang, Ming-Zhen Yang, Ying Zhuo, Ying-Ning Zheng, Cheng-Yi Xiong, Ya-Qin Chai, Ruo Yuan
A simple and highly-efficient approach to monitor the expression of P-glycoprotein (P-gp) in cells was urgently needed to demonstrate the drug resistance of cancer cells. Herein, a competitive method-based electrochemiluminescent (ECL) assay with a single ECL indicator was proposed for the first time to efficiently estimate the concentration ratio of two proteins. By converting the different proteins to partially coincident nucleotide sequences via a sandwich type immunoassay on magnetic beads, the concentration ratio related ECL signals could be obtained via competitive nucleotide hybridization on an electrode surface...
December 1, 2016: Chemical Science
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