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Ultrasensitive immunoassay

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https://www.readbyqxmd.com/read/28443852/a-biosensor-based-on-self-clickable-aiegen-a-signal-amplification-strategy-for-ultrasensitive-immunoassays
#1
Youyong Yuan, Wenbo Wu, Shidang Xu, Bin Liu
A signal amplified fluorogenic ELISA based on self-clickable fluorogens with aggregation-induced emission characteristics (AIEgen) as a substrate was developed for ultrasensitive immunoassay.
April 26, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28438806/emerging-affinity-based-proteomic-technologies-for-large-scale-plasma-profiling-in-cardiovascular-disease
#2
REVIEW
J Gustav Smith, Robert E Gerszten
Plasma biomarkers that reflect molecular states of the cardiovascular system are central for clinical decision making. Routinely used plasma biomarkers include troponins, natriuretic peptides, and lipoprotein particles, yet interrogate only a modest subset of pathways relevant to cardiovascular disease. Systematic profiling of a larger portion of circulating plasma proteins (the plasma proteome) will provide opportunities for unbiased discovery of novel markers to improve diagnostic or predictive accuracy. In addition, proteomic profiling may inform pathophysiological understanding and point to novel therapeutic targets...
April 25, 2017: Circulation
https://www.readbyqxmd.com/read/28432339/multifunctional-substrate-of-label-free-electrochemical-immunosensor-for-ultrasensitive-detection-of-cytokeratins-antigen-21-1
#3
Huiqiang Wang, Xin Gao, Zhanfang Ma
Poly(thionine)-Au, a novel multifunctional substrate with excellent redox signal, enzyme-like activity, and easy antibody immobilisation, was synthesised using HAuCl4 as the oxidising agent and thionine as the monomer. The prepared poly(thionine)-Au composite exhibited an admirable electrochemical redox signal at -0.15 V and excellent H2O2 catalytic ability. In addition, gold nanoparticles in this composite were found to directly immobilise antibodies and further improve conductivity. In addition, a label-free electrochemical immunosensor was developed using poly(thionine)-Au as the sensing substrate for ultrasensitive detection of cytokeratin antigen 21-1 (CYFRA 21-1), an immunoassay found in human serum...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28411532/one-pot-loading-high-content-thionine-on-polydopamine-functionalized-mesoporous-silica-nanosphere-for-ultrasensitive-electrochemical-immunoassay
#4
Guosong Lai, Min Zheng, Wenjie Hu, Aimin Yu
A thionine (TH)-doped mesoporous silica nanosphere (MSN)/polydopamine (PDA) nanocomposite was synthesized for developing a new signal transduction strategy of electrochemical immunoassay. This nanocomposite was synthesized through one-pot loading of TH and in situ formation of PDA-coating on MSN. After antibody labeling, the obtained nanoprobe was used for the signal tracing of sandwich immunoassay at a magnetic bead-assay platform. Based on the specific capture of the MSN-TH/PDA nanoprobes through sandwich immunoreaction to form a magnetic immunocomplex and the following treatment of the immunocomplex with a NaOH solution, the PDA film coated on MSN was destroyed to release TH tags from the nanoprobe...
April 11, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28411223/modified-bacteriophage-s16-long-tail-fiber-proteins-for-rapid-and-specific-immobilization-and-detection-of-salmonella-cells
#5
Jenna M Denyes, Matthew Dunne, Stanislava Steiner, Maximilian Mittelviefhaus, Agnes Weiss, Herbert Schmidt, Jochen Klumpp, Martin J Loessner
Bacteriophage-based assays and biosensors rival traditional antibody-based immunoassays for detection of low-level Salmonella contaminations. In this study, we harnessed the binding specificity of the long tail fiber (LTF) from bacteriophage S16 as an affinity molecule for immobilization, enrichment, and detection of Salmonella We demonstrate that paramagnetic beads (MBs) coated with recombinant gp37-gp38 LTF complexes (LTF-MBs) are highly effective tools for rapid affinity magnetic separation and enrichment of Salmonella Within 45 minutes, the LTF-MBs consistently captured over 95% of Salmonella Typhimurium cells from suspensions containing 10-10(5) CFU...
April 14, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28391867/ultrasensitive-immunoassay-for-monocrotaline-using-monoclonal-antibody-produced-by-n-n-carbonyldiimidazole-mediated-hapten-carrier-protein-conjugates
#6
Seiichi Sakamoto, Rika Nagamitsu, Gorawit Yusakul, Tomofumi Miyamoto, Hiroyuki Tanaka, Satoshi Morimoto
Monocrotaline (MCT) is a naturally occurring pyrrolizidine alkaloid mainly produced in plant genus Crotalaria (Fabaceae), in which various toxicities have been reported. Recently, demand of quantitative analysis for determination/detection of MCT has increased because MCT in plants can be transferred to human by food chain, leading to intoxication. In this study, monoclonal antibody (MAb) against MCT (MAb 4B8) was produced by unique one-pot conjugation between hydrolysate of MCT, retronecine (RN) and consecutive lysine residues of bovine-serum albumin (BSA) through N,N'-carbonyldiimidazole (CDI) mediated method...
June 1, 2017: Talanta
https://www.readbyqxmd.com/read/28382160/sensitive-detection-of-protein-biomarkers-using-silver-nanoparticles-enhanced-immunofluorescence-assay
#7
Li-Jun Zhao, Ru-Jia Yu, Wei Ma, Huan-Xing Han, He Tian, Ruo-Can Qian, Yi-Tao Long
Detection of biomarkers is extremely important for the early diagnosis of diseases. Here, we developed an easy and highly sensitive fluorescence detection system for the determination of biomarkers by combining the rapid separation of magnetic beads and silver nanoparticles labeled antibodies. An ultrasensitive silver ions fluorescence probe 3', 6'-bis (diethylamino)-2-(2-iodoethyl) spiro[isoindoline-1, 9'-xanthen]-3-one (Ag(+)-FP) was applied to immunoassay. A significant signal amplification was achieved as the AgNPs can be dissolved by H2O2 and generate numerous Ag(+), which would turn "on" the fluorescence of Ag(+)-FP...
2017: Theranostics
https://www.readbyqxmd.com/read/28335966/a-concentration-dependent-multicolor-conversion-strategy-for-ultrasensitive-colorimetric-immunoassay-with-the-naked-eye
#8
Yingshuai Liu, Zeying Zhang, Jie Yu, Jin Xie, Chang Ming Li
Colorimetric immunoassays have been attracting more attention for use in practical applications, especially in point-of-care diagnostics. In comparison with a single color immunoassay, the dose-dependent multicolor strategy greatly improves the detection resolution and accuracy of visual inspection. In the current study, a concentration-dependent multicolor conversion strategy was developed based on gold nanoparticle (AuNP)-mediated copper deposition for signal amplification and Prussian blue for color generation...
April 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28317772/ultrasensitive-detection-of-clenbuterol-by-a-covalent-imprinted-polymer-as-a-biomimetic-antibody
#9
Yiwei Tang, Jingwen Gao, Xiuying Liu, Xue Gao, Tao Ma, Xiaonan Lu, Jianrong Li
As an ideal biomimetic antibody, molecularly imprinted polymer (MIP) has shown great promise in immunoassays. Here, we developed a covalent imprinting method to prepare MIP artificial antibody towards clenbuterol on the well surface of a microtiter plate. Template molecule (clenbuterol) was splited by hydrolysis with functional monomer and removed by methanol/acetic acid solution, and then three-dimensional cavities were fabricated in the MIP, which can rebind template molecule via hydrogen bond interaction...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28315213/enzyme-linked-immunosorbent-assay-elisa
#10
George N Konstantinou
Food allergy is a public health concern especially after recognizing its constantly increased prevalence and severity. Despite careful reading of food ingredient statements, food allergic individuals may experience reactions caused by "hidden", "masked", or "contaminated" proteins that are known major allergens. Many techniques have been developed to detect even small traces of food allergens, for clinical or laboratory purposes. Enzyme-linked immunosorbent assay (ELISA) is one of the best validated and most routinely used immunoassay in allergy research, in allergy diagnosis in allergy-related quality control in various industries...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28290193/molecularly-imprinted-plasmonic-substrates-for-specific-and-ultrasensitive-immunoassay-of-trace-glycoproteins-in-biological-samples
#11
Pir Muhammad, Xueying Tu, Jia Liu, Yijia Wang, Zhen Liu
Assays of glycoproteins hold significant biological importance and clinical values, for which immunoassay has been the workhorse tool. As immunoassays are associated with disadvantages such as poor availability of high-specificity antibodies, limited stability of biological reagents, and tedious procedure, innovative alternatives that can overcome these drawbacks are highly desirable. Plasmonic immunosandwich assay (PISA) has emerged as an appealing alternative to immunoassay for fast and sensitive determination of trace glycoproteins in biosamples...
March 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28225095/high-index-hk0-faceted-platinum-concave-nanocubes-with-enhanced-peroxidase-like-activity-for-an-ultrasensitive-colorimetric-immunoassay-of-the-human-prostate-specific-antigen
#12
Zhuangqiang Gao, Shuzhen Lv, Mingdi Xu, Dianping Tang
Developing simple, high-efficiency non-enzyme bioassays is of great importance for modern analytical systems, but remains a significant challenge. One promising route is to utilize highly efficient nanocatalysts with the exposure of active crystal facets. Herein, we for the first time propose a novel colorimetric immunoassay for the ultrasensitive detection of the human prostate-specific antigen (PSA) based on using a unique type of nanolabel - high-index {hk0} faceted platinum concave nanocubes (HIF-Pt-CNCs)...
March 13, 2017: Analyst
https://www.readbyqxmd.com/read/28208287/highly-uniform-gold-nanobipyramids-for-ultrasensitive-colorimetric-detection-of-influenza-virus
#13
Shaohua Xu, Wenjun Ouyang, Peisi Xie, Yi Lin, Bin Qiu, Zhenyu Lin, Guonan Chen, Longhua Guo
Gold nanoparticles (AuNPs) have been frequently utilized for the construction of diverse colorimetric biosensors. Normally, AuNPs with sharp edges could have better sensitivity. However, the poor monodipersity of AuNPs with sharp edges seriously confines their utility for colorimetric biosensing. Herein, we demonstrate the utility of highly uniform gold nanobipyramids (Au NBPs) for ultrasensitive colorimetric detection of H5N1 virus. The proposed method is based on the fact that alkaline phosphatase (ALP) could catalyze the decomposition of 4-aminophenyl phosphate (4-APP) to generate 4-aminophenol (4-AP), which would then reduce silver nitrate to metal silver and then deposited on Au NBPs...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28199410/ultrasensitive-detection-of-hiv-1-p24-antigen-by-a-hybrid-nanomechanical-optoplasmonic-platform-with-potential-for-detecting-hiv-1-at-first-week-after-infection
#14
Priscila M Kosaka, Valerio Pini, Montserrat Calleja, Javier Tamayo
Early detection of HIV infection is the best way to prevent spread of the disease and to improve the efficiency of the antiretroviral therapy. Nucleic acid amplification tests (NAAT) have become the gold-standard for detecting low-concentrations of the virus in blood. However, these methods are technically demanding and cost-prohibitive in developing countries. Immunoassays are more affordable and can be more easily adapted for point-of-care diagnosis. However, the sensitivity so far of these methods has been too low...
2017: PloS One
https://www.readbyqxmd.com/read/28193039/molecularly-imprinted-polymer-based-plasmonic-immunosandwich-assay-for-fast-and-ultrasensitive-determination-of-trace-glycoproteins-in-complex-samples
#15
Xueying Tu, Pir Muhammad, Jia Liu, Yanyan Ma, Shuangshou Wang, Danyang Yin, Zhen Liu
Glycoproteins play significant roles in many biological processes. Assays of glycoproteins have significant biological importance and clinical values, for which immunoassay has been the workhorse tool. However, immunoassay suffers from some disadvantages, such as poor availability of high-specificity antibodies and limited stability of biological reagents. Herein, we present an antibody-free and enzyme-free approach, called molecularly imprinted polymer (MIP)-based plasmonic immunosandwich assay (PISA), for fast and ultrasensitive detection of trace glycoproteins in complex samples...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28193012/metal-organic-framework-nanomaterials-as-novel-signal-probes-for-electron-transfer-mediated-ultrasensitive-electrochemical-immunoassay
#16
Ting-Zhi Liu, Rong Hu, Xi Zhang, Kun-Lei Zhang, Yi Liu, Xiao-Bing Zhang, Ru-Yan Bai, Delei Li, Yun-Hui Yang
A novel and simple electrochemical immunoassay for C-reactive protein was developed using metal-organic frameworks (Au-MOFs) as signal unit. In this study, we found MOFs could be used as signal probe. And this new class of signal probe differs from traditional probe. The signal of the copper ions (Cu(2+)) from MOFs could be directly detected without acid dissolution and preconcentration, which would greatly simplify the detection steps and reduce the detection time. Moreover, MOFs contain large amounts of Cu(2+) ions, providing high electrochemical signals...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28182976/ultrasensitive-electrochemical-immunosensor-for-quantitative-detection-of-scca-using-co3o4-ceo2-au-pt-nanocomposite-as-enzyme-mimetic-labels
#17
Yueyun Li, Yihe Zhang, Faying Li, Jinhui Feng, Mingdang Li, Lei Chen, Yunhui Dong
Recently early diagnosis of squamous cell carcinoma antigen (SCCA) as a tumor maker of various cancers has increasingly attracted a lot of attention with heightening of incidence rate of cancer. The SCCA with low concentration in human serum should be diluted before detecting. Thus, an immunoassay with high sensitivity is significant for early detecting SCCA. Therefore, a nonenzymatic sandwich-type electrochemical immunosensor herein was conducted to quantitative detection of squamous cell carcinoma antigen (SCCA)...
June 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28155925/an-enzyme-induced-au-ag-core-shell-nanostructure-used-for-an-ultrasensitive-surface-enhanced-raman-scattering-immunoassay-of-cancer-biomarkers
#18
Lin Yang, Ming Xuan Gao, Lei Zhan, Min Gong, Shu Jun Zhen, Cheng Zhi Huang
Early detection of cancer is helpful for the control and prevention of diseases. Due to the low content of cancer biomarkers in the early disease phases, however, an ultrasensitive and selective method is critical. In this contribution, an ultrasensitive surface-enhanced Raman scattering (SERS) immunoassay is newly developed with the principle of introducing a common enzyme-induced deposition (EID) reaction to coat a silver layer on the surface of gold nanoparticles and to form a core-shell nanostructure of Au@Ag...
February 16, 2017: Nanoscale
https://www.readbyqxmd.com/read/28150210/an-ultrasensitive-biosensing-platform-employing-acetylcholinesterase-and-gold-nanoparticles
#19
Dingbin Liu, Xiaoyuan Chen
Enzyme-linked immunosorbent assay (ELISA) is a well-known strategy for biomarker detection with a color change, which can be seen by the naked eyes. However, the moderate sensitivity of conventional ELISA limits its applications in many cases where the concentrations of biomarker are very low, such as cancer diagnosis. Here we describe an ultrasensitive colorimetric assay based on acetylcholinesterase (AChE)-catalyzed hydrolysis reaction, whose products trigger the aggregation of gold nanoparticles (AuNPs), causing a distinct color change of the solution from red to purple...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28112761/au-ag-serrs-tags-coupled-to-a-lateral-flow-immunoassay-for-the-sensitive-detection-of-pneumolysin
#20
Lucía Blanco-Covián, Verónica Montes-García, Alexandre Girard, M Teresa Fernández-Abedul, Jorge Pérez-Juste, Isabel Pastoriza-Santos, Karen Faulds, Duncan Graham, M Carmen Blanco-López
Establishing a definitive diagnosis of pneumonia using conventional tests is difficult and expensive. Lateral flow immunoassays (LFIAs) are an advantageous point of care (POC) test option, but they have some limitations in terms of detection and quantification. In this work we have developed a lateral flow immunoassay for the ultrasensitive detection of penumolysin employing plasmonic Surface-Enhanced Resonance Raman Scattering (SERRS) tag as labelled probe. The combination of Au@Ag core-shell nanoparticles as plasmonic platform and Rhodamine B Isothiocyanate as Raman reporter has allowed us to fabricate a SERRS tag with high efficiency and reliability...
January 23, 2017: Nanoscale
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