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Ultrasensitive immunoassay

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https://www.readbyqxmd.com/read/29324284/a-novel-cytosensor-based-on-pt-ag-nanoflowers-and-aunps-acetylene-black-for-ultrasensitive-and-highly-specific-detection-of-circulating-tumor-cells
#1
Sitian Tang, Huawei Shen, Yixiong Hao, Zhenglan Huang, Yiyi Tao, Yang Peng, Yongcan Guo, Guoming Xie, Wenli Feng
Circulating tumor cells (CTCs), as the cellular origin of metastasis, are cancer cells that break away from a primary tumor and circulate in the peripheral blood. And they provide a wealth of information about tumor phenotype. Here, this work reported a novel ultrasensitive immunoassay protocol for the detection of CTCs by using Pt@Ag nanoflowers (Pt@AgNFs) and AuNPs/Acetylene black (AuNPs/AB) nanomaterial. In the established approach, AuNPs/AB nanomaterial was used as substrate material to increase the specific surface area and enhance the conductivity of the gold electrode...
January 4, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29310262/nano-biotinylated-liposome-based-immunoassay-for-the-ultrasensitive-detection-of-protein-biomarker-in-urine
#2
Peng Liu, Xueen Fang, Hongmei Cao, Mingli Gu, Jilie Kong, Anmei Deng
With the development of proteomics and the continuous discovery of biomarkers of trace proteins, it is important to accurately quantify low abundance protein, especially in urine for clinical diagnostics. In this paper, we reported a novel nano-biotinylated liposome-based immuno-loop-mediated isothermal amplification (LI-LAMP) for the ultrasensitive detection of REG1A (a biomarker for pancreatic ductal adenocarcinoma (PDAC) in urine) with high specificity. The detection range was 1µg/mL to 1fg/mL, with a detection limit of 1fg/mL, and no cross-reactivity was observed to occur in this assay...
March 1, 2018: Talanta
https://www.readbyqxmd.com/read/29299908/fluorescent-immunoassay-for-the-detection-of-pathogenic-bacteria-at-the-single-cell-level-using-carbon-dots-encapsulated-breakable-organosilica-nanocapsule-as-labels
#3
Lu Yang, Wenfang Deng, Chang Cheng, Yueming Tan, Qingji Xie, Shouzhuo Yao
Herein, carbon dots (CDs)-encapsulated breakable organosilica nanocapsules (BONs) were facilely prepared and used as advanced fluorescent labels for ultrasensitive detection of Staphylococcus Aureus (S. Aureus). CDs were entrapped in organosilica shells by co-hydrolyzation of tetraethylorthosilicate and bis[3-(triethoxysilyl)propyl]disulfide to form core-shell CDs@BONs, where hundreds of CDs are encapsulated in each nanocapsule. Immunofluorescent nanocapsules, i.e. anti-S. Aureus antibody-conjugated CDs@BONs were prepared to specifically recognize S...
January 4, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29299559/ultrasensitive-electrochemical-immunoassay-for-melanoma-cells-using-mesoporous-polyaniline
#4
M U Anu Prathap, Carlos Iván Rodríguez, Omer Sadak, Jiehao Guan, Vijayasaradhi Setaluri, Sundaram Gunasekaran
We report the development of an antibody (anti-MC1R antibody)-functionalized polyaniline nanofibers modified screen-printed electrode capable of efficient electrochemical detection of melanoma cells at levels (1 cell per mL) not readily achieved by other methods. This immunosensor is highly selective in its detection of melanoma cells over normal human cells.
January 4, 2018: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29289826/a-sandwich-type-electrochemical-immunoassay-for-ultrasensitive-detection-of-non-small-cell-lung-cancer-biomarker-cyfra21-1
#5
Yan Zeng, Jing Bao, Yanan Zhao, Danqun Huo, Mei Chen, Yanli Qi, Mei Yang, Huanbao Fa, Changjun Hou
Many studies confirm that the aberrant expression of Cytokeratin 19 fragment 21-1 (CYFRA21-1) is highly correlated with non-small cell lung cancer (NSCLC), especially for squamous cell carcinoma. Herein, we report a sandwich-type electrochemical immunosensor based on signal amplification strategy of multiple nanocomposites to test CYFRA21-1 selectively and sensitively. The proposed immunosensor fabricated by three-dimensional graphene (3D-G), chitosan (CS) and glutaraldehyde (GA) composite on the glass carbon electrode (GCE) with a large surface area is prepared to immobilize primary antibodies (Ab1) and provide excellent conductivity...
November 13, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/29260556/digital-single-virus-electrochemical-enzyme-linked-immunoassay-for-ultrasensitive-h7n9-avian-influenza-virus-counting
#6
Zhen Wu, Wen-Jing Guo, Yi-Yan Bai, Li Zhang, Jiao Hu, Dai-Wen Pang, Zhi-Ling Zhang
Electrochemistry has been widely used to explore fundamental properties of single molecules due to its fast response and high specificity. However, the lack of efficient signal amplification strategies and quantitative method limit its clinical application. Here, we proposed a digital single virus electrochemical enzyme-linked immunoassay (digital ELISA) for H7N9 avian influenza virus (H7N9 AIV) counting by integration of digital analysis, bifunctional fluorescence magnetic nanospheres (bi-FMNs) with monolayer gold nanoparticles (Au NPs) modified microelectrode array (MA)...
December 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29258827/a-carbon-nanotube-enhanced-real-time-immuno-pcr-for-ultrasensitive-detection-of-ahtn-in-water
#7
Xiaohan Zhang, Huisheng Zhuang
Polycyclic musks (PCMs) in the aquatic environment have become an emerging environmental issue because of their potential risk. The most commonly used method for analysis of PCMs is gas chromatography-mass spectrometer (GC-MS) with different sample extractions, which are somewhat expensive to operate, laborious and complex. In this paper, a carbon nanotube-enhanced real time immuno-PCR was developed for ultrasensitive detection of AHTN in water for the first time. The SWCNTs were used to immobilize numerous amino-DNA and polyclonal antibody to form polyclonal antibody-CNTs-DNA conjugates, which were used as a signal-amplifier in the proposed immunoassay system...
December 16, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29236046/advances-in-proteomic-techniques-for-cytokine-analysis-focus-on-melanoma-research
#8
REVIEW
Helena Kupcova Skalnikova, Jana Cizkova, Jakub Cervenka, Petr Vodicka
Melanoma is a skin cancer with permanently increasing incidence and resistance to therapies in advanced stages. Reports of spontaneous regression and tumour infiltration with T-lymphocytes makes melanoma candidate for immunotherapies. Cytokines are key factors regulating immune response and intercellular communication in tumour microenvironment. Cytokines may be used in therapy of melanoma to modulate immune response. Cytokines also possess diagnostic and prognostic potential and cytokine production may reflect effects of immunotherapies...
December 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29223051/ultrasensitive-colorimetric-immunoassay-for-hcg-detection-based-on-dual-catalysis-of-au-pt-core-shell-nanoparticle-functionalized-by-horseradish-peroxidase
#9
Weiguo Wang, Yake Zou, Jinwu Yan, Jing Liu, Huixiong Chen, Shan Li, Lei Zhang
In this paper, an ultrasensitive colorimetric biosensor for human chorionic gonadotrophin (hCG) detection was designed from bottom-up method based on the dual catalysis of the horseradish peroxidase (HRP) and Au@Pt nanoparticles (NPs) relative to H2O2-TEM system. HRP and monoclonal mouse anti-hCG antibody (β-submit, mAb1) were co-immobilized onto the Au@Pt NP surface to improve catalytic efficiency and specificity, which formed a dual functionalized Au@Pt-HRP probe with the mean size of 42.8nm (D50). The colorimetric immunoassay was developed for the hCG detection, and the Au@Pt-HRP probe featured a higher sensitivity in the concentration range of 0...
December 5, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29215864/platinum-nanocatalyst-amplification-redefining-the-gold-standard-for-lateral-flow-immunoassays-with-ultra-broad-dynamic-range
#10
Colleen N Loynachan, Michael R Thomas, Eleanor R Gray, Daniel A Richards, Jeongyun Kim, Benjamin S Miller, Jennifer Clare Brookes, Shweta Agarwal, Vijay Chudasama, Rachel A McKendry, Molly M Stevens
Paper-based lateral flow immunoassays (LFIAs) are one of the most widely used point-of-care (PoC) devices, however, their application in early disease diagnostics is often limited due to insufficient sensitivity for the requisite sample sizes and the short timeframes of PoC testing. To address this, we developed a serum-stable, nanoparticle catalyst-labelled LFIA with a sensitivity surpassing that of both current commercial and published sensitivities for paper-based detection of p24, one of the earliest and most conserved biomarkers of HIV...
December 7, 2017: ACS Nano
https://www.readbyqxmd.com/read/29204292/serum-neurofilament-light-chain-in-nmosd-and-related-disorders-comparison-according-to-aquaporin-4-and-myelin-oligodendrocyte-glycoprotein-antibodies-status
#11
Mariotto S, Farinazzo A, Monaco S, Gajofatto A, Zanusso G, Schanda K, Capra R, Mancinelli C, Bonora A, Bombardi R, Reindl M, Ferrari S
Background: Neurofilament light chain (NF-L) levels reflect axonal damage in different conditions, including demyelinating disorders. Objectives: We aimed to compare serum NF-L levels in patients with aquaporin-4 antibodies (AQP4-Ab), myelin oligodendrocyte antibodies (MOG-Ab) and seronegative cases with neuromyelitis optica spectrum disorders and related disorders. Methods: We analysed AQP4-Ab and MOG-Ab with cell-based assay and NF-L with ultrasensitive electrochemiluminescence immunoassay...
October 2017: Multiple Sclerosis Journal—Experimental, Translational and Clinical
https://www.readbyqxmd.com/read/29197558/clinical-utility-of-an-ultrasensitive-late-night-salivary-cortisol-assay-by-tandem-mass-spectrometry
#12
Lillian R Sturmer, Dylan Dodd, Christina S Chao, Run-Zhang Shi
BACKGROUND: Late night salivary cortisol measurement is a clinically important and convenient screening test for Cushing's syndrome. Tandem mass spectrometry (LC-MS/MS) assays have superior sensitivity and specificity compared to immunoassays. Our goal was to improve a LC-MS/MS method to measure salivary cortisol in both adult and pediatric patients and to characterize its analytical performance by method validation and clinical performance by chart review. METHODS: We improved a LC-MS/MS method originally developed for urine cortisol to measure low level salivary cortisol...
November 29, 2017: Steroids
https://www.readbyqxmd.com/read/29195715/utilization-of-a-lateral-flow-colloidal-gold-immunoassay-strip-based-on-surface-enhanced-raman-spectroscopy-for-ultrasensitive-detection-of-antibiotics-in-milk
#13
Qiaoqiao Shi, Jie Huang, Yaning Sun, Mengqi Yin, Mei Hu, Xiaofei Hu, Zhijun Zhang, Gaiping Zhang
An ultrasensitive method for the detection of antibiotics in milk is developed based on inexpensive, simple, rapid and portable lateral flow immunoassay (LFI) strip, in combination with high sensitivity surface-enhanced Raman spectroscopy (SERS). In our strategy, an immunoprobe was prepared from colloidal gold (AuNPs) conjugated with both a monoclonal antibody against neomycin (NEO-mAb) and a Raman probe molecule 4-aminothiophenol (PATP). The competitive interaction with immunoprobe between free NEO and the coated antigen (NEO-OVA) resulted in the change of the amount of the immobilized immunoprobe on the paper substrate...
November 21, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29190730/increased-levels-of-plasma-total-tau-in-adult-down-syndrome
#14
Takashi Kasai, Harutsugu Tatebe, Masaki Kondo, Ryotaro Ishii, Takuma Ohmichi, Wing Tung Esther Yeung, Masafumi Morimoto, Tomohiro Chiyonobu, Naoto Terada, David Allsop, Masanori Nakagawa, Toshiki Mizuno, Takahiko Tokuda
Down syndrome (DS) is the most prevalent chromosomal abnormality. Early-onset dementia with the pathology of Alzheimer's disease (AD) frequently develops in DS. Reliable blood biomarkers are needed to support the diagnosis for dementia in DS, since positron emission tomography or cerebrospinal fluid sampling is burdensome, particularly for patients with DS. Plasma t-tau is one of the established biomarkers for the diagnosis of AD, suggesting the potential value of t-tau as a biomarker for dementia in DS. The aim of this study was to assess and compare plasma levels of t-tau in adults with DS and in an age-matched control population...
2017: PloS One
https://www.readbyqxmd.com/read/29162692/phosphorylation-of-huntingtin-at-residue-t3-is-decreased-in-huntington-s-disease-and-modulates-mutant-huntingtin-protein-conformation
#15
Cristina Cariulo, Lucia Azzollini, Margherita Verani, Paola Martufi, Roberto Boggio, Anass Chiki, Sean M Deguire, Marta Cherubini, Silvia Gines, J Lawrence Marsh, Paola Conforti, Elena Cattaneo, Iolanda Santimone, Ferdinando Squitieri, Hilal A Lashuel, Lara Petricca, Andrea Caricasole
Posttranslational modifications can have profound effects on the biological and biophysical properties of proteins associated with misfolding and aggregation. However, their detection and quantification in clinical samples and an understanding of the mechanisms underlying the pathological properties of misfolding- and aggregation-prone proteins remain a challenge for diagnostics and therapeutics development. We have applied an ultrasensitive immunoassay platform to develop and validate a quantitative assay for detecting a posttranslational modification (phosphorylation at residue T3) of a protein associated with polyglutamine repeat expansion, namely Huntingtin, and characterized its presence in a variety of preclinical and clinical samples...
November 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29149995/facile-synthesis-of-polyaniline-polythionine-redox-hydrogel-conductive-antifouling-and-enzyme-linked-material-for-ultrasensitive-label-free-amperometric-immunosensor-toward-carcinoma-antigen-125
#16
Lihua Zhao, Zhanfang Ma
Sensitivity enhancement and proteins adsorption are the common challenges faced in protein immunoassays. In this work, an ultrasensitive and protein-resistant label-free amperometric immunosening platform for carcinoma antigen-125 (CA125) based on redox polyaniline-polythionine hydrogel (PANI-PThi gel) was developed. The as-prepared hydrogel, which was facilely synthesized by electropolymerization, exhibited good conductivity and strong hydrophilicity while the sensitivity and specificity of the immunosensor can be enhanced...
January 2, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29140390/a-carboxylated-graphene-nanodisks-glucose-oxidase-nanotags-and-mn-cds-tio2-matrix-based-dual-signal-amplification-strategy-for-ultrasensitive-photoelectrochemical-detection-of-tumor-markers
#17
Jie Song, Shuo Wu, Xinlan Yang, Jingli Yuan
Graphene nanodisks with good conductivity and plenty of edge sites were synthesized to load glucose oxidase (GRD-GOD) and coupled with a Mn(2+) doped CdS quantum dot (QD) modified TiO2 electrode (CdS:Mn/TiO2) for a highly sensitive photoelectrochemical (PEC) immunoassay. The specific immune-recognition behaviour can bring the GRD-GOD labelled antigen into the antibody immobilized CdS:Mn/TiO2 interface and dramatically enhance the photocurrent response via a dual signal amplification strategy. First, graphene nanodisks with a strong electron transfer capacity can improve the conductivity of both the insulating protein layers and the CdS:Mn/TiO2 matrix, thus facilitating the regeneration of trapped carriers and hot electrons in the CdS:Mn QD films and enhancing the PEC performance...
November 15, 2017: Analyst
https://www.readbyqxmd.com/read/29126472/a-novel-visible-light-driven-photoelectrochemical-immunosensor-based-on-multi-amplification-strategy-for-ultrasensitive-detection-of-microcystin-lr
#18
Jie Wei, Aori Qileng, Yun Yan, Hongtao Lei, Shengsen Zhang, Weipeng Liu, Yingju Liu
An ultrasensitive photoelectrochemical (PEC) immunoassay based on multiple signal amplification strategy was fabricated for the detection of microcystin-LR (MC-LR). The CdS/TiO2 nanorod arrays (CdS/TiO2 NRAs) modified FTO electrode, which can weaken the self-oxidation by photogenerated holes of CdS nanoparticles, and limit the recombination of electron-hole pairs and broaden optical absorption of TiO2 NRAs, was used as a visible-light driven material to immobilize antigens. Then, Fe3O4 nanoparticles/polydopamine (Fe3O4@PDA) was used as the carrier to load secondary antibody (Ab2) and horseradish peroxidase (HRP), where Fe3O4 nanoparticles and HRP can synergistically accelerate the oxidation of 4-chloro-1-naphthol (4-CN) by H2O2 to produce biocatalytic precipitation (BCP) on the surface of modified electrode...
November 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29125493/validation-of-ultrasensitive-mutant-huntingtin-detection-in-human-cerebrospinal-fluid-by-single-molecule-counting-immunoassay
#19
Valentina Fodale, Roberto Boggio, Manuel Daldin, Cristina Cariulo, Maria Carolina Spiezia, Lauren Mary Byrne, Blair R Leavitt, Edward Wild, Douglas Macdonald, Andreas Weiss, Alberto Bresciani
BACKGROUND: The measurement of disease-relevant biomarkers has become a major component of clinical trial design, but in the absence of rigorous clinical and analytical validation of detection methodology, interpretation of results may be misleading. In Huntington's disease (HD), measurement of the concentration of mutant huntingtin protein (mHTT) in cerebrospinal fluid (CSF) of patients may serve as both a disease progression biomarker and a pharmacodynamic readout for HTT-lowering therapeutic approaches...
November 9, 2017: Journal of Huntington's Disease
https://www.readbyqxmd.com/read/29096364/an-ultrasensitive-electrochemiluminescent-immunosensor-based-on-graphene-oxide-coupled-graphite-like-carbon-nitride-and-multiwalled-carbon-nanotubes-gold-for-the-detection-of-diclofenac
#20
Liuyi Hu, Jing Zheng, Kang Zhao, Anping Deng, Jianguo Li
In this study, a novel competition-type electrochemiluminescent (ECL) immunosensor for detecting diclofenac (DCF) was fabricated with graphene oxide coupled graphite-like carbon nitride (GO-g-C3N4) as signal probe for the first time. The ECL intensity of carboxylated g-C3N4 was significantly enhanced after being combined with graphene oxide (GO) which exhibited excellent charge-transport property. The sensing platform was constructed by multiwalled carbon nanotubes and gold nanoparticles (MWCNTs-AuNPs), which not only provided an effective matrix for immobilizing a large amount of coating antigen but also facilitated the electronic transmission rate to enhance the ECL intensity...
October 19, 2017: Biosensors & Bioelectronics
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