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https://www.readbyqxmd.com/read/28920155/distributions-of-recent-gullies-on-hillslopes-with-different-slopes-and-aspects-in-the-black-soil-region-of-northeast-china
#1
Dichen Wang, Haoming Fan, Xiangguo Fan
Gully erosion is an important environmental problem worldwide and the main process by which water and soil losses occur in the Black Soil Region (BSR) of Northeast China. At the end of 2012, 295,663 gullies were present in this region. However, few studies have examined the gullies of the Black Soil Region as a whole. Studying the distribution of recent gullies can reveal the pattern of gully distribution and can help predict their spatial development according to the soil and water conservation regionalization of China...
September 17, 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28905113/influence-of-increased-heart-rate-and-aortic-pressure-on-resting-indices-of-functional-coronary-stenosis-severity
#2
Lorena Casadonte, Bart-Jan Verhoeff, Jan J Piek, Ed VanBavel, Jos A E Spaan, Maria Siebes
Baseline assessment of functional stenosis severity has been proposed as a practical alternative to hyperemic indices. However, intact autoregulation mechanisms may affect intracoronary hemodynamics. The aim of this study was to investigate the effect of changes in aortic pressure (Pa) and heart rate (HR) on baseline coronary hemodynamics and functional stenosis assessment. In 15 patients (55 ± 3% diameter stenosis) Pa, intracoronary pressure (Pd) and flow velocity were obtained at control, and during atrial pacing at 120 bpm, increased Pa (+30 mmHg) with intravenous phenylephrine (PE), and elevated Pa while pacing at sinus heart rate (PE + sHR)...
September 13, 2017: Basic Research in Cardiology
https://www.readbyqxmd.com/read/28894454/fine-mapping-of-a-clubroot-resistance-gene-in-chinese-cabbage-using-snp-markers-identified-from-bulked-segregant-rna-sequencing
#3
Zhen Huang, Gary Peng, Xunjia Liu, Abhinandan Deora, Kevin C Falk, Bruce D Gossen, Mary R McDonald, Fengqun Yu
Clubroot, caused by Plasmodiophora brassicae, is an important disease of canola (Brassica napus) in western Canada and worldwide. In this study, a clubroot resistance gene (Rcr2) was identified and fine mapped in Chinese cabbage cv. "Jazz" using single-nucleotide polymorphisms (SNP) markers identified from bulked segregant RNA sequencing (BSR-Seq) and molecular markers were developed for use in marker assisted selection. In total, 203.9 million raw reads were generated from one pooled resistant (R) and one pooled susceptible (S) sample, and >173,000 polymorphic SNP sites were identified between the R and S samples...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28888655/the-matrix-protein-of-vesicular-stomatitis-virus-inhibits-host-directed-transcription-of-target-genes-via-interaction-with-the-tfiih-subunit-p8
#4
Wei Pan, Deguang Song, Wenqi He, Huijun Lu, Yungang Lan, Juanzhen Tong, Feng Gao, Kui Zhao
In response to viral infection, the host innate antiviral response is elicited to limit viral replication. Many viruses have evolved various strategies to circumvent the host antiviral response. It has been reported that matrix (M) protein of vesicular stomatitis virus (VSV) can inhibit host gene expression to evade the host innate immune response. However, the molecular mechanism remains unclear. Here, we demonstrated that VSV M protein inhibited transcription of a reporter gene transfected into BSR-T7/5 cells...
September 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28879499/impaired-dopamine-dependent-locomotory-behavior-of-c-elegans-neuroligin-mutants-depends-on-the-catechol-o-methyltransferase-comt-4
#5
Ángel Rodríguez-Ramos, M Mar Gámez-Del-Estal, Montserrat Porta-de-la-Riva, Julián Cerón, Manuel Ruiz-Rubio
Neurexins and neuroligins are neuronal membrane adhesion molecules that have been involved in neuropsychiatric and neurodevelopmental disorders. The nrx-1 and nlg-1 genes of Caenorhabditis elegans encode NRX-1 and NLG-1, orthologue proteins of human neurexins and neuroligins, respectively. Dopaminergic and serotoninergic signalling control the locomotory rate of the nematode. When well-fed animals are transferred to a plate with food (bacterial lawn), they reduce the locomotory rate. This behavior, which depends on dopamine, is known as basal slowing response (BSR)...
September 6, 2017: Behavior Genetics
https://www.readbyqxmd.com/read/28842906/application-of-split-gfp-reassembly-assay-to-study-myogenesis-and-myofusion-in-vitro
#6
Manami Kodaka, Xiaoyin Xu, Zeyu Yang, Junichi Maruyama, Yutaka Hata
Green fluorescent protein (GFP) is composed of 11 β-strands, and loses GFP signals, when divided into the N-terminal ten β-strands (GFP1-10) and the C-terminal last β-strand (GFP11). However, when GFP1-10 and GFP11 encounter, they reassemble into the fluorescent GFP. We expressed GFP1-10 and blasticidin resistance gene product-fused GFP11 (BSR-GFP11) in C2C12 cells. Both the cell lines do not show GFP but when they undergo myogenesis and myofusion, GFP1-10 and BSR-GFP11 form the fluorescent complex in multi-nuclear myotubes, so that GFP signals reflect myogenesis and myofusion...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28822816/genetically-modified-rabies-virus-vectored-ebola-virus-disease-vaccines-are-safe-and-induce-efficacious-immune-responses-in-mice-and-dogs
#7
Lei Shuai, Xijun Wang, Zhiyuan Wen, Jinying Ge, Jinliang Wang, Dandan Zhao, Zhigao Bu
Ebola viruses (EBOVs) are zoonotic pathogens that cause EBOV disease (EVD) with high case fatality in humans. Currently, EVD vaccines are still under development in several countries. Here, we generated two recombinant rabies viruses (RABVs), rERAG333E/ZGP and rERAG333E/SGP, expressing the Zaire EBOV glycoprotein (ZGP) or Sudan EBOV glycoprotein (SGP) gene based on a modified ERA vaccine strain (rERAG333E) vector platform. The recombinant RABVs retained growth properties similar to those of the vector virus in BSR cell culture and efficiently expressed ZGP or SGP...
August 16, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28736352/the-effect-of-biocomposite-screws-on-bone-regeneration-in-a-rat-osteoporosis-model
#8
Je Hoon Jeong, Eun-Sun Jin, Ji Yeon Kim, Bora Lee, JoongKee Min, Sang Ryong Jeon, Minsu Lee, Kyoung Hyo Choi
OBJECTIVE: To evaluate the efficacy of biocomposite screws used in ovariectomy (OVX)-induced osteoporotic rats. METHODS: Twenty-four female Wistar rats (250-300 g, 12 weeks old) were divided into 4 groups: sham group (control), OVX-induced osteoporosis group (OVX), OVX and biodegradable poly(lactic-co-glycolic acid) (PLGA) without tricalcium phosphate (β-TCP) screw insertion group (OVX/BSR), and OVX and biocomposite (PLGA with β-TCP) screw insertion group (OVX/CSR)...
July 20, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28726621/characterization-of-fitzroy-river-virus-and-serologic-evidence-of-human-and-animal-infection
#9
Cheryl A Johansen, Simon H Williams, Lorna F Melville, Jay Nicholson, Roy A Hall, Helle Bielefeldt-Ohmann, Natalie A Prow, Glenys R Chidlow, Shani Wong, Rohini Sinha, David T Williams, W Ian Lipkin, David W Smith
In northern Western Australia in 2011 and 2012, surveillance detected a novel arbovirus in mosquitoes. Genetic and phenotypic analyses confirmed that the new flavivirus, named Fitzroy River virus, is related to Sepik virus and Wesselsbron virus, in the yellow fever virus group. Most (81%) isolates came from Aedes normanensis mosquitoes, providing circumstantial evidence of the probable vector. In cell culture, Fitzroy River virus replicated in mosquito (C6/36), mammalian (Vero, PSEK, and BSR), and avian (DF-1) cells...
August 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28711956/mapping-stripe-rust-resistance-gene-yrzh22-in-chinese-wheat-cultivar-zhoumai-22-by-bulked-segregant-rna-seq-bsr-seq-and-comparative-genomics-analyses
#10
Yong Wang, Jingzhong Xie, Huaizhi Zhang, Bingmin Guo, Shunzong Ning, Yongxing Chen, Ping Lu, Qiuhong Wu, Miaomiao Li, Deyun Zhang, Guanghao Guo, Yan Zhang, Dengcai Liu, Shaokui Zou, Jianwei Tang, Hong Zhao, Xicheng Wang, Jun Li, Wuyun Yang, Tingjie Cao, Guihong Yin, Zhiyong Liu
A stripe rust resistance gene YrZH22 was mapped by combined BSR-Seq and comparative genomics analyses to a 5.92 centimorgan (cM) genetic interval spanning a 4 Mb physical genomic region on wheat chromosome 4BL1. Stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most destructive diseases of wheat and severely threatens wheat production worldwide. The widely grown Chinese wheat cultivar Zhoumai 22 is highly resistant to the current prevailing PST race CYR34 (V26). Genetic analysis of F5:6 and F6:7 recombinant inbred line (RIL) populations indicated that adult-plant stripe rust resistance in Zhoumai 22 is controlled by a single gene, temporarily designated YrZH22...
July 15, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28676679/disinhibited-eating-mediates-differences-in-attachment-insecurity-between-bariatric-surgery-candidates-recipients-and-lean-controls
#11
L L Wilkinson, A C Rowe, C Sheldon, A Johnson, J M Brunstrom
Previous research has shown that attachment anxiety is a good predictor of body mass index (BMI). This relationship is significantly mediated by disinhibited (over-) eating and is likely to reflect a specific form of affect regulation. This study explored whether obese bariatric surgery candidates (BSC; N=34) and bariatric surgery recipients (BSR; N=15) would show higher levels of attachment insecurity (higher attachment anxiety and/or higher attachment avoidance) than a group of age and gender-matched lean controls (N=54)...
July 5, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/28672276/biological-stress-reactivity-as-an-index-of-the-two-polarities-of-the-experience-model
#12
Jaime R Silva, Anastassia Vivanco-Carlevari, Mauricio Barrientos, Claudio Martínez, Luis A Salazar, Mariane Krause
The two-polarities model of personality argues that experience is organized around two axes: interpersonal relatedness and self-definition. Differential emphasis on one of these poles defines adaptive and pathological experiences, generating anaclitic or introjective tendencies. The anaclitic pattern, on one hand, has been conceptually related with an exaggerated emphasis on interpersonal relatedness. On the other hand, the introjective pattern has been connected to high levels of self-criticism. The aim of this study was to investigate the psychophysiological basis for this relationship...
June 24, 2017: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/28666113/a-natural-allele-of-a-transcription-factor-in-rice-confers-broad-spectrum-blast-resistance
#13
Weitao Li, Ziwei Zhu, Mawsheng Chern, Junjie Yin, Chao Yang, Li Ran, Mengping Cheng, Min He, Kang Wang, Jing Wang, Xiaogang Zhou, Xiaobo Zhu, Zhixiong Chen, Jichun Wang, Wen Zhao, Bingtian Ma, Peng Qin, Weilan Chen, Yuping Wang, Jiali Liu, Wenming Wang, Xianjun Wu, Ping Li, Jirui Wang, Lihuang Zhu, Shigui Li, Xuewei Chen
Rice feeds half the world's population, and rice blast is often a destructive disease that results in significant crop loss. Non-race-specific resistance has been more effective in controlling crop diseases than race-specific resistance because of its broad spectrum and durability. Through a genome-wide association study, we report the identification of a natural allele of a C2H2-type transcription factor in rice that confers non-race-specific resistance to blast. A survey of 3,000 sequenced rice genomes reveals that this allele exists in 10% of rice, suggesting that this favorable trait has been selected through breeding...
June 29, 2017: Cell
https://www.readbyqxmd.com/read/28663795/temporal-artery-biopsy-in-the-diagnosis-of-giant-cell-arteritis-does-the-end-justify-the-means
#14
K Bowling, J Rait, J Atkinson, G Srinivas
BACKGROUND: Early temporal artery biopsy is recommended in all patients with suspected cranial GCA (Giant Cell Arteritis) by the BSR (British Society of Rheumatology) and BHPR (British Health Professionals in Rheumatology) guidelines. This should be performed within one week ideally. AIM: To assess ACR (American College of Rheumatology) score at presentation and whether temporal artery biopsy result affects clinical management of the clinically suspected GCA patient...
August 2017: Annals of Medicine and Surgery
https://www.readbyqxmd.com/read/28659961/bulked-segregant-rna-seq-reveals-differential-expression-and-snps-of-candidate-genes-associated-with-waterlogging-tolerance-in-maize
#15
Hewei Du, Jianxiong Zhu, Hang Su, Ming Huang, Hongwei Wang, Shuangcheng Ding, Binglin Zhang, An Luo, Shudong Wei, Xiaohai Tian, Yunbi Xu
Waterlogging has increasingly become one of the major constraints to maize productivity in some maize production zones because it causes serious yield loss. Bulked segregant RNA-seq (BSR-seq) has been widely applied to profile candidate genes and map associated Single Nucleotide Polymorphism (SNP) markers in many species. In this study, 10 waterlogging sensitive and eight tolerant inbred lines were selected from 60 maize inbred lines with waterlogging response determined and preselected by the International Maize and Wheat Improvement Center (CIMMYT) from over 400 tropical maize inbred lines...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28651190/temporal-dynamics-of-the-compositions-and-activities-of-soil-microbial-communities-post-application-of-the-insecticide-chlorantraniliprole-in-paddy-soils
#16
Meng Wu, Jia Liu, Weitao Li, Ming Liu, Chunyu Jiang, Zhongpei Li
Chlorantraniliprole (CAP) is a newly developed insecticide widely used in rice fields in China. There has been few studies evaluating the toxicological effects of CAP on soil-associated microbes. An 85-day microcosm experiment was performed to reveal the dissipation dynamics of CAP in three types of paddy soils in subtropical China. The effects of CAP on microbial activities (microbial biomass carbon-MBC, basal soil respiration-BSR, microbial metabolic quotient-qCO2, acid phosphatase and sucrose invertase activities) in the soils were periodically evaluated...
June 23, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28611468/insights-into-enterotoxigenic-escherichia-coli-diversity-in-bangladesh-utilizing-genomic-epidemiology
#17
Jason W Sahl, Jeticia R Sistrunk, Nabilah Ibnat Baby, Yasmin Begum, Qingwei Luo, Alaullah Sheikh, Firdausi Qadri, James M Fleckenstein, David A Rasko
Enterotoxigenic Escherichia coli (ETEC) cause more than 500,000 deaths each year in the developing world and are characterized on a molecular level by the presence of genes that encode the heat-stable (ST) and/or heat-labile (LT) enterotoxins, as well as surface structures, known as colonization factors (CFs). Genome sequencing and comparative genomic analyses of 94 previously uncharacterized ETEC isolates demonstrated remarkable genomic diversity, with 28 distinct sequence types identified in three phylogenomic groups...
June 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28592651/identification-of-nitrogen-consumption-genetic-variants-in-yeast-through-qtl-mapping-and-bulk-segregant-rna-seq-analyses
#18
Francisco A Cubillos, Claire Brice, Jennifer Molinet, Sebastién Tisné, Valentina Abarca, Sebastián M Tapia, Christian Oporto, Verónica García, Gianni Liti, Claudio Martínez
Saccharomyces cerevisiae is responsible for wine must fermentation. In this process, nitrogen represents a limiting nutrient and its scarcity results in important economic losses for the wine industry. Yeast isolates use different strategies to grow in poor nitrogen environments and their genomic plasticity enables adaptation to multiple habitats through improvements in nitrogen consumption. Here, we used a highly recombinant S. cerevisiae multi-parent population (SGRP-4X) derived from the intercross of four parental strains of different origins to identify new genetic variants responsible for nitrogen consumption differences during wine fermentation...
June 7, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28589507/biliary-stone-removal-through-the-percutaneous-transhepatic-biliary-drainage-route-focusing-on-the-balloon-sphincteroplasty-flushing-technique-a-single-center-study-with-916-patients
#19
Jong Soo Shin, Hyung Jin Shim, Byung Kook Kwak, Hyun-Ki Yoon
PURPOSE: To present our experience in biliary stone removal (BSR) through the percutaneous transhepatic biliary drainage (PTBD) route in 916 patients, and discuss its clinical usefulness. MATERIALS AND METHODS: From 2001 to 2015, 916 patients (479 male patients and 437 female patients; age range, 22-92 years; mean age, 67 years) with 52 recurring cases, so a total of 968 cases, were enrolled in this study and retrospectively reviewed. PTBD was performed in all patients...
August 2017: Japanese Journal of Radiology
https://www.readbyqxmd.com/read/28562673/analysis-of-parb-centromere-interactions-by-multiplex-spr-imaging-reveals-specific-patterns-for-binding-parb-in-six-centromeres-of-burkholderiales-chromosomes-and-plasmids
#20
Flavien Pillet, Fanny Marie Passot, Franck Pasta, Véronique Anton Leberre, Jean-Yves Bouet
Bacterial centromeres-also called parS, are cis-acting DNA sequences which, together with the proteins ParA and ParB, are involved in the segregation of chromosomes and plasmids. The specific binding of ParB to parS nucleates the assembly of a large ParB/DNA complex from which ParA-the motor protein, segregates the sister replicons. Closely related families of partition systems, called Bsr, were identified on the chromosomes and large plasmids of the multi-chromosomal bacterium Burkholderia cenocepacia and other species from the order Burkholeriales...
2017: PloS One
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