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https://www.readbyqxmd.com/read/29142128/interaction-between-a-unique-minor-protein-and-a-major-capsid-protein-of-bluetongue-virus-controls-virus-infectivity
#1
Eiko Matsuo, Kiyoshi Yamazaki, Hiroki Tsuruta, Polly Roy
Among the Reoviridae family of double-stranded RNA viruses, only members of the Orbivirus genus possess a unique structural protein, termed VP6, within their particles. Bluetongue virus (BTV), an important livestock pathogen, is the prototype Orbivirus The BTV VP6 is an ATP-dependent RNA helicase and it is indispensable for virus replication. In this report, we investigated how VP6 might be recruited to the virus capsid and whether the BTV structural protein VP3, which forms the internal layer of the virus capsid core, is involved in VP6 recruitment...
November 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29132085/how-clear-cutting-affects-fire-severity-and-soil-properties-in-a-mediterranean-ecosystem
#2
Marcos Francos, Paulo Pereira, Jorge Mataix-Solera, Victoria Arcenegui, Meritxell Alcañiz, Xavier Úbeda
Forest management practices in Mediterranean ecosystems are frequently employed to reduce both the risk and severity of wildfires. However, these pre-fire treatments may influence the effects of wildfire events on soil properties. The aim of this study is to examine the short-term effects of a wildfire that broke out in 2015 on the soil properties of three sites: two exposed to management practices in different years - 2005 (site M05B) and 2015 (site M15B) - and one that did not undergo any management (NMB) and to compare their properties with those recorded in a plot (Control) unaffected by the 2015 wildfire...
November 10, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/29029272/bsr-and-bhpr-guideline-for-the-prescription-and-monitoring-of-non-biologic-disease-modifying-anti-rheumatic-drugs
#3
Jo Ledingham, Nicola Gullick, Katherine Irving, Rachel Gorodkin, Melissa Aris, Jean Burke, Patrick Gordon, Dimitrios Christidis, Sarah Galloway, Eranga Hayes, Andrew Jeffries, Scott Mercer, Janice Mooney, Sander van Leuven, James Galloway
No abstract text is available yet for this article.
September 28, 2017: Rheumatology
https://www.readbyqxmd.com/read/28977764/comparative-genomic-and-plasmid-analysis-of-beer-spoiling-and-non-beer-spoiling-lactobacillus-brevis-isolates
#4
Jordyn Bergsveinson, Barry Ziola
Beer-spoilage-related lactic acid bacteria (BSR LAB) belong to multiple genera and species; however, beer-spoilage capacity is isolate-specific and partially acquired via horizontal gene transfer within the brewing environment. Thus, the extent to which genus-, species-, or environment- (i.e., brewery-) level genetic variability influences beer-spoilage phenotype is unknown. Publicly available Lactobacillus brevis genomes were analyzed via BlAst Diagnostic Gene findEr (BADGE) for BSR genes and assessed for pangenomic relationships...
October 4, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28944759/optimization-of-an-ultralow-dose-high-resolution-pediatric-pet-scanner-design-based-on-monolithic-scintillators-with-dual-sided-digital-sipm-readout-a-simulation-study
#5
Ekaterina Mikhaylova, Valerio Tabacchini, Giacomo Borghi, Pieter Mollet, Ester D'Hoe, Dennis R Schaart, Stefaan Vandenberghe
The goal of this simulation study is the performance evaluation and comparison of six potential designs for a time-of-flight PET scanner for pediatric patients of up to about 12 years of age. It is designed to have a high sensitivity and provide high-contrast and high-resolution images. The simulated pediatric PET is a full ring scanner, consisting of 32  ×  32 mm(2) monolithic LYSO:Ce crystals coupled to digital silicon photomultiplier arrays. The six considered designs differ in axial lengths (27...
October 19, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28920155/distributions-of-recent-gullies-on-hillslopes-with-different-slopes-and-aspects-in-the-black-soil-region-of-northeast-china
#6
Dichen Wang, Haoming Fan, Xiangguo Fan
Gully erosion is an important environmental problem worldwide and the main process by which water and soil losses occur in the Black Soil Region (BSR) of Northeast China. At the end of 2012, 295,663 gullies were present in this region. However, few studies have examined the gullies of the Black Soil Region as a whole. Studying the distribution of recent gullies can reveal the pattern of gully distribution and can help predict their spatial development according to the soil and water conservation regionalization of China...
September 17, 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28905113/influence-of-increased-heart-rate-and-aortic-pressure-on-resting-indices-of-functional-coronary-stenosis-severity
#7
Lorena Casadonte, Bart-Jan Verhoeff, Jan J Piek, Ed VanBavel, Jos A E Spaan, Maria Siebes
Baseline assessment of functional stenosis severity has been proposed as a practical alternative to hyperemic indices. However, intact autoregulation mechanisms may affect intracoronary hemodynamics. The aim of this study was to investigate the effect of changes in aortic pressure (Pa) and heart rate (HR) on baseline coronary hemodynamics and functional stenosis assessment. In 15 patients (55 ± 3% diameter stenosis) Pa, intracoronary pressure (Pd) and flow velocity were obtained at control, and during atrial pacing at 120 bpm, increased Pa (+30 mmHg) with intravenous phenylephrine (PE), and elevated Pa while pacing at sinus heart rate (PE + sHR)...
September 13, 2017: Basic Research in Cardiology
https://www.readbyqxmd.com/read/28894454/fine-mapping-of-a-clubroot-resistance-gene-in-chinese-cabbage-using-snp-markers-identified-from-bulked-segregant-rna-sequencing
#8
Zhen Huang, Gary Peng, Xunjia Liu, Abhinandan Deora, Kevin C Falk, Bruce D Gossen, Mary R McDonald, Fengqun Yu
Clubroot, caused by Plasmodiophora brassicae, is an important disease of canola (Brassica napus) in western Canada and worldwide. In this study, a clubroot resistance gene (Rcr2) was identified and fine mapped in Chinese cabbage cv. "Jazz" using single-nucleotide polymorphisms (SNP) markers identified from bulked segregant RNA sequencing (BSR-Seq) and molecular markers were developed for use in marker assisted selection. In total, 203.9 million raw reads were generated from one pooled resistant (R) and one pooled susceptible (S) sample, and >173,000 polymorphic SNP sites were identified between the R and S samples...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28888655/the-matrix-protein-of-vesicular-stomatitis-virus-inhibits-host-directed-transcription-of-target-genes-via-interaction-with-the-tfiih-subunit-p8
#9
Wei Pan, Deguang Song, Wenqi He, Huijun Lu, Yungang Lan, Juanzhen Tong, Feng Gao, Kui Zhao
In response to viral infection, the host innate antiviral response is elicited to limit viral replication. Many viruses have evolved various strategies to circumvent the host antiviral response. It has been reported that matrix (M) protein of vesicular stomatitis virus (VSV) can inhibit host gene expression to evade the host innate immune response. However, the molecular mechanism remains unclear. Here, we demonstrated that VSV M protein inhibited transcription of a reporter gene transfected into BSR-T7/5 cells...
September 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28879499/impaired-dopamine-dependent-locomotory-behavior-of-c-elegans-neuroligin-mutants-depends-on-the-catechol-o-methyltransferase-comt-4
#10
Ángel Rodríguez-Ramos, M Mar Gámez-Del-Estal, Montserrat Porta-de-la-Riva, Julián Cerón, Manuel Ruiz-Rubio
Neurexins and neuroligins are neuronal membrane adhesion molecules that have been involved in neuropsychiatric and neurodevelopmental disorders. The nrx-1 and nlg-1 genes of Caenorhabditis elegans encode NRX-1 and NLG-1, orthologue proteins of human neurexins and neuroligins, respectively. Dopaminergic and serotoninergic signalling control the locomotory rate of the nematode. When well-fed animals are transferred to a plate with food (bacterial lawn), they reduce the locomotory rate. This behavior, which depends on dopamine, is known as basal slowing response (BSR)...
September 6, 2017: Behavior Genetics
https://www.readbyqxmd.com/read/28842906/application-of-split-gfp-reassembly-assay-to-study-myogenesis-and-myofusion-in-vitro
#11
Manami Kodaka, Xiaoyin Xu, Zeyu Yang, Junichi Maruyama, Yutaka Hata
Green fluorescent protein (GFP) is composed of 11 β-strands, and loses GFP signals, when divided into the N-terminal ten β-strands (GFP1-10) and the C-terminal last β-strand (GFP11). However, when GFP1-10 and GFP11 encounter, they reassemble into the fluorescent GFP. We expressed GFP1-10 and blasticidin resistance gene product-fused GFP11 (BSR-GFP11) in C2C12 cells. Both the cell lines do not show GFP but when they undergo myogenesis and myofusion, GFP1-10 and BSR-GFP11 form the fluorescent complex in multi-nuclear myotubes, so that GFP signals reflect myogenesis and myofusion...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28822816/genetically-modified-rabies-virus-vectored-ebola-virus-disease-vaccines-are-safe-and-induce-efficacious-immune-responses-in-mice-and-dogs
#12
Lei Shuai, Xijun Wang, Zhiyuan Wen, Jinying Ge, Jinliang Wang, Dandan Zhao, Zhigao Bu
Ebola viruses (EBOVs) are zoonotic pathogens that cause EBOV disease (EVD) with high case fatality in humans. Currently, EVD vaccines are still under development in several countries. Here, we generated two recombinant rabies viruses (RABVs), rERAG333E/ZGP and rERAG333E/SGP, expressing the Zaire EBOV glycoprotein (ZGP) or Sudan EBOV glycoprotein (SGP) gene based on a modified ERA vaccine strain (rERAG333E) vector platform. The recombinant RABVs retained growth properties similar to those of the vector virus in BSR cell culture and efficiently expressed ZGP or SGP...
August 16, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28736352/the-effect-of-biocomposite-screws-on-bone-regeneration-in-a-rat-osteoporosis-model
#13
Je Hoon Jeong, Eun-Sun Jin, Ji Yeon Kim, Bora Lee, JoongKee Min, Sang Ryong Jeon, Minsu Lee, Kyoung Hyo Choi
OBJECTIVE: To evaluate the efficacy of biocomposite screws used in ovariectomy (OVX)-induced osteoporotic rats. METHODS: Twenty-four female Wistar rats (250-300 g, 12 weeks old) were divided into 4 groups: sham group (control), OVX-induced osteoporosis group (OVX), OVX and biodegradable poly(lactic-co-glycolic acid) (PLGA) without tricalcium phosphate (β-TCP) screw insertion group (OVX/BSR), and OVX and biocomposite (PLGA with β-TCP) screw insertion group (OVX/CSR)...
October 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28726621/characterization-of-fitzroy-river-virus-and-serologic-evidence-of-human-and-animal-infection
#14
Cheryl A Johansen, Simon H Williams, Lorna F Melville, Jay Nicholson, Roy A Hall, Helle Bielefeldt-Ohmann, Natalie A Prow, Glenys R Chidlow, Shani Wong, Rohini Sinha, David T Williams, W Ian Lipkin, David W Smith
In northern Western Australia in 2011 and 2012, surveillance detected a novel arbovirus in mosquitoes. Genetic and phenotypic analyses confirmed that the new flavivirus, named Fitzroy River virus, is related to Sepik virus and Wesselsbron virus, in the yellow fever virus group. Most (81%) isolates came from Aedes normanensis mosquitoes, providing circumstantial evidence of the probable vector. In cell culture, Fitzroy River virus replicated in mosquito (C6/36), mammalian (Vero, PSEK, and BSR), and avian (DF-1) cells...
August 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28711956/mapping-stripe-rust-resistance-gene-yrzh22-in-chinese-wheat-cultivar-zhoumai-22-by-bulked-segregant-rna-seq-bsr-seq-and-comparative-genomics-analyses
#15
Yong Wang, Jingzhong Xie, Huaizhi Zhang, Bingmin Guo, Shunzong Ning, Yongxing Chen, Ping Lu, Qiuhong Wu, Miaomiao Li, Deyun Zhang, Guanghao Guo, Yan Zhang, Dengcai Liu, Shaokui Zou, Jianwei Tang, Hong Zhao, Xicheng Wang, Jun Li, Wuyun Yang, Tingjie Cao, Guihong Yin, Zhiyong Liu
A stripe rust resistance gene YrZH22 was mapped by combined BSR-Seq and comparative genomics analyses to a 5.92 centimorgan (cM) genetic interval spanning a 4 Mb physical genomic region on wheat chromosome 4BL1. Stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most destructive diseases of wheat and severely threatens wheat production worldwide. The widely grown Chinese wheat cultivar Zhoumai 22 is highly resistant to the current prevailing PST race CYR34 (V26). Genetic analysis of F5:6 and F6:7 recombinant inbred line (RIL) populations indicated that adult-plant stripe rust resistance in Zhoumai 22 is controlled by a single gene, temporarily designated YrZH22...
October 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28676679/disinhibited-eating-mediates-differences-in-attachment-insecurity-between-bariatric-surgery-candidates-recipients-and-lean-controls
#16
L L Wilkinson, A C Rowe, C Sheldon, A Johnson, J M Brunstrom
Previous research has shown that attachment anxiety is a good predictor of body mass index (BMI). This relationship is significantly mediated by disinhibited (over-) eating and is likely to reflect a specific form of affect regulation. This study explored whether obese bariatric surgery candidates (BSC; N=34) and bariatric surgery recipients (BSR; N=15) would show higher levels of attachment insecurity (higher attachment anxiety and/or higher attachment avoidance) than a group of age and gender-matched lean controls (N=54)...
July 5, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/28672276/biological-stress-reactivity-as-an-index-of-the-two-polarities-of-the-experience-model
#17
Jaime R Silva, Anastassia Vivanco-Carlevari, Mauricio Barrientos, Claudio Martínez, Luis A Salazar, Mariane Krause
The two-polarities model of personality argues that experience is organized around two axes: interpersonal relatedness and self-definition. Differential emphasis on one of these poles defines adaptive and pathological experiences, generating anaclitic or introjective tendencies. The anaclitic pattern, on one hand, has been conceptually related with an exaggerated emphasis on interpersonal relatedness. On the other hand, the introjective pattern has been connected to high levels of self-criticism. The aim of this study was to investigate the psychophysiological basis for this relationship...
June 24, 2017: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/28666113/a-natural-allele-of-a-transcription-factor-in-rice-confers-broad-spectrum-blast-resistance
#18
Weitao Li, Ziwei Zhu, Mawsheng Chern, Junjie Yin, Chao Yang, Li Ran, Mengping Cheng, Min He, Kang Wang, Jing Wang, Xiaogang Zhou, Xiaobo Zhu, Zhixiong Chen, Jichun Wang, Wen Zhao, Bingtian Ma, Peng Qin, Weilan Chen, Yuping Wang, Jiali Liu, Wenming Wang, Xianjun Wu, Ping Li, Jirui Wang, Lihuang Zhu, Shigui Li, Xuewei Chen
Rice feeds half the world's population, and rice blast is often a destructive disease that results in significant crop loss. Non-race-specific resistance has been more effective in controlling crop diseases than race-specific resistance because of its broad spectrum and durability. Through a genome-wide association study, we report the identification of a natural allele of a C2H2-type transcription factor in rice that confers non-race-specific resistance to blast. A survey of 3,000 sequenced rice genomes reveals that this allele exists in 10% of rice, suggesting that this favorable trait has been selected through breeding...
June 29, 2017: Cell
https://www.readbyqxmd.com/read/28663795/temporal-artery-biopsy-in-the-diagnosis-of-giant-cell-arteritis-does-the-end-justify-the-means
#19
K Bowling, J Rait, J Atkinson, G Srinivas
BACKGROUND: Early temporal artery biopsy is recommended in all patients with suspected cranial GCA (Giant Cell Arteritis) by the BSR (British Society of Rheumatology) and BHPR (British Health Professionals in Rheumatology) guidelines. This should be performed within one week ideally. AIM: To assess ACR (American College of Rheumatology) score at presentation and whether temporal artery biopsy result affects clinical management of the clinically suspected GCA patient...
August 2017: Annals of Medicine and Surgery
https://www.readbyqxmd.com/read/28659961/bulked-segregant-rna-seq-reveals-differential-expression-and-snps-of-candidate-genes-associated-with-waterlogging-tolerance-in-maize
#20
Hewei Du, Jianxiong Zhu, Hang Su, Ming Huang, Hongwei Wang, Shuangcheng Ding, Binglin Zhang, An Luo, Shudong Wei, Xiaohai Tian, Yunbi Xu
Waterlogging has increasingly become one of the major constraints to maize productivity in some maize production zones because it causes serious yield loss. Bulked segregant RNA-seq (BSR-seq) has been widely applied to profile candidate genes and map associated Single Nucleotide Polymorphism (SNP) markers in many species. In this study, 10 waterlogging sensitive and eight tolerant inbred lines were selected from 60 maize inbred lines with waterlogging response determined and preselected by the International Maize and Wheat Improvement Center (CIMMYT) from over 400 tropical maize inbred lines...
2017: Frontiers in Plant Science
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