Read by QxMD icon Read


Peipei Wu, Jingzhong Xie, Jinghuang Hu, Dan Qiu, Zhiyong Liu, Jingting Li, Miaomiao Li, Hongjun Zhang, Li Yang, Hongwei Liu, Yang Zhou, Zhongjun Zhang, Hongjie Li
Powdery mildew resistance gene Pm4b , originating from Triticum persicum , is effective against the prevalent Blumeria graminis f. sp. tritici ( Bgt ) isolates from certain regions of wheat production in China. The lack of tightly linked molecular markers with the target gene prevents the precise identification of Pm4b during the application of molecular marker-assisted selection (MAS). The strategy that combines the RNA-Seq technique and the bulked segregant analysis (BSR-Seq) was applied in an F2:3 mapping population (237 families) derived from a pair of isogenic lines VPM1/7∗ Bainong 3217 F4 (carrying Pm4b ) and Bainong 3217 to develop more closely linked molecular markers...
2018: Frontiers in Plant Science
Veera Mohana Rao Kakita, Kanaka Mahalakshmi Jerripothula, Sahithya Phani Babu Vemulapalli, Jagadeesh Bharatam
JHH scalar couplings carry rich structural information and their measurements are fundamental in the1 H NMR based elucidation of small and medium molecules, which, however are hampered in the presence of large J-coupling network. Further, enhanced spectral resolution is often essential for precise determination of a specific set of1 H-1 H J-couplings amongst the complex J-multiplets. In the light of the recent advancements in homodecoupling pure shift strategies, here we report absorption mode, band-selective refocused pure shift spin-echo (BSR-PSSE) method, which helps in determining1 H-1 H J-couplings from crowded spectral regions...
February 27, 2018: Magnetic Resonance in Chemistry: MRC
Qiaomei Wu, Mingyang Ding, Chuanfeng Li, Guangqing Liu, Zongyan Chen
Novel duck reovirus (NDRV), the prototype strain of the species Avian orthoreovirus (ARV), is currently an infectious agent for ducks. Studies on NDRV replication and pathogenesis have been hampered by the lack of an available reverse-genetics system. In this study, a plasmid-based reverse-genetics system that is free of helper viruses has been developed. In this system, 10 full-length gene segments of wild-type NDRV TH11 strain are transfected into BSR-T7/5 cells that express bacteriophage T7 RNA polymerase...
February 26, 2018: Journal of General Virology
Michelle E Kurt-Mangold, Caleb V Grieme, Matthew D Krasowski, Nancy S Rosenthal
BACKGROUND: Blood smear reviews by pathologists (BSR) are ordered frequently at our institution, take time to evaluate, and result in a written report. Minimal research has been done regarding the amount of novel data reported and its clinical utility. METHODS: Detailed chart review was performed on BSR orders from January 1, 2015 to March 31, 2015 to assess reasons for smear review, if results were mentioned in the chart, if laboratory-driven reviews were already performed, and if novel, clinically influential data was reported...
January 1, 2018: Clinical Laboratory
Lin Chen, To-Hung Tsui, George A Ekama, Hamish R Mackey, Tianwei Hao, Guanghao Chen
The determination of organics biodegradability and corresponding biodegradation kinetics provides valuable information on the optimal design and operation of anaerobic biotechnology especially for sulfidogenesis. This study proposes a deterministic method, i.e. a biochemical sulfide potential (BSP) test, and compares it to the conventional biochemical methane potential (BMP) test in terms of their ability to characterize sulfate-laden organic waste biodegradability. It demonstrated 1.48 times higher degradation of volatile suspended solids (VSS) and 2...
February 9, 2018: Water Research
Xiaogang Zhou, Haicheng Liao, Mawsheng Chern, Junjie Yin, Yufei Chen, Jianping Wang, Xiaobo Zhu, Zhixiong Chen, Can Yuan, Wen Zhao, Jing Wang, Weitao Li, Min He, Bingtian Ma, Jichun Wang, Peng Qin, Weilan Chen, Yuping Wang, Jiali Liu, Yangwen Qian, Wenming Wang, Xianjun Wu, Ping Li, Lihuang Zhu, Shigui Li, Pamela C Ronald, Xuewei Chen
Crops carrying broad-spectrum resistance loci provide an effective strategy for controlling infectious disease because these loci typically confer resistance to diverse races of a pathogen or even multiple species of pathogens. Despite their importance, only a few crop broad-spectrum resistance loci have been reported. Here, we report the identification and characterization of the rice bsr-k1 (broad-spectrum resistance Kitaake-1) mutant, which confers broad-spectrum resistance against Magnaporthe oryzae and Xanthomonas oryzae pv oryzae with no major penalty on key agronomic traits...
February 5, 2018: Proceedings of the National Academy of Sciences of the United States of America
Ana M Bossa-Castro, Cheick Tekete, Chitra Raghavan, Emily E Delorean, Alexis Dereeper, Karim Dagno, Ousmane Koita, Gloria Mosquera, Hei Leung, Valérie Verdier, Jan E Leach
Quantitative trait loci (QTL) that confer broad-spectrum resistance (BSR), or resistance that is effective against multiple and diverse plant pathogens, have been elusive targets of crop breeding programs. Multi-parent Advanced Generation Inter-Cross (MAGIC) populations, with their diverse genetic composition and high levels of recombination, are potential resources for identification of QTL for BSR. In this study, a rice MAGIC population was used to map QTL conferring BSR to two major rice diseases, bacterial leaf streak (BLS) and bacterial blight (BB), caused by Xanthomonas oryzae pathovars (pv...
February 6, 2018: Plant Biotechnology Journal
Arthy Surendran, Yasmeen Siddiqui, Nusaibah Syd Ali, Sivakumar Manickam
AIM: Ganoderma sp, the causal pathogen of the basal stem rot (BSR) disease of oil palm, secrets extracellular hydrolytic enzymes. Which play an important role in the pathogenesis of BSR by nourishing the pathogen through the digestion of cellulose and hemicellulose of the host tissue. Active suppression of hydrolytic enzymes secreted by G.boninense by various naturally occurring phenolic compounds and estimation of their efficacy on pathogen suppression is focused in this study. METHODS AND RESULTS: Ten naturally occurring phenolic compounds were assessed for their inhibitory effect on the hydrolytic enzymes of G...
February 5, 2018: Journal of Applied Microbiology
Jacob A Lasala, Colin R Hughes, Jeanette Wyneken
Species that display temperature-dependent sex determination are at risk as a result of increasing global temperatures. For marine turtles, high incubation temperatures can skew sex ratios towards females. There are concerns that temperature increases may result in highly female-biased offspring sex ratios, which would drive a future sex ratio skew. Studying the sex ratios of adults in the ocean is logistically very difficult because individuals are widely distributed and males are inaccessible because they remain in the ocean...
2018: PloS One
(no author information available yet)
Gout is the most common form of inflammatory arthritis and its incidence in the UK has steadily increased from 1.5% in 1997 to 2.5% in 2012.1,2 It is characterised by deposition of monosodium urate crystals in joints and tissues and usually presents with intermittent painful attacks followed by long periods of remission.3 It has been suggested that the management of gout in the UK remains suboptimal.1 In 2004, we concluded that there was a woeful lack of evidence to guide treatment or prophylaxis for gout, particularly with regard to choice of drug or doses...
January 2018: Drug and Therapeutics Bulletin
Adina R Bujold, Andrew E Shure, Rui Liu, Andrew M Kropinski, Janet I MacInnes
Actinobacillus spp. are Gram-negative bacteria associated with mucosal membranes. While some are commensals, others can cause important human and animal diseases. A. pleuropneumoniae causes severe fibrinous hemorrhagic pneumonia in swine but not systemic disease whereas other species invade resulting in septicemia and death. To understand the invasive phenotype of Actinobacillus spp., complete genomes of eight isolates were obtained and pseudogenomes of five isolates were assembled and annotated. Phylogenetically, A...
January 6, 2018: Genomics
Isshadiba Mustafa, Mohd Zobir Hussein, Bullo Saifullah, Abu Seman Idris, Nur Hailini Zainol Hilmi, Sharida Fakurazi
Fungicide, namely hexaconazole was successfully intercalated into the intergalleries of zinc/aluminium-layered double hydroxide (ZALDH) using ion exchange method. Due to the intercalation of hexaconazole, the basal spacing of the ZALDH was increased from 8.7 Å in ZALDH to 29.45 Ǻ in hexaconazole-intercalated ZALDH (HZALDH). The intercalation of hexaconazole into the interlayer of the nanocomposite was confirmed using the Fourier-transform infrared (FTIR) study. This supramolecular chemistry intercalation process enhanced the thermal stability of the hexaconazole moiety...
December 28, 2017: Journal of Agricultural and Food Chemistry
Eiko Matsuo, Kiyoshi Yamazaki, Hiroki Tsuruta, Polly Roy
Among the Reoviridae family of double-stranded RNA viruses, only members of the Orbivirus genus possess a unique structural protein, termed VP6, within their particles. Bluetongue virus (BTV), an important livestock pathogen, is the prototype Orbivirus The BTV VP6 is an ATP-dependent RNA helicase and it is indispensable for virus replication. In this report, we investigated how VP6 might be recruited to the virus capsid and whether the BTV structural protein VP3, which forms the internal layer of the virus capsid core, is involved in VP6 recruitment...
November 15, 2017: Journal of Virology
Marcos Francos, Paulo Pereira, Jorge Mataix-Solera, Victoria Arcenegui, Meritxell Alcañiz, Xavier Úbeda
Forest management practices in Mediterranean ecosystems are frequently employed to reduce both the risk and severity of wildfires. However, these pre-fire treatments may influence the effects of wildfire events on soil properties. The aim of this study is to examine the short-term effects of a wildfire that broke out in 2015 on the soil properties of three sites: two exposed to management practices in different years - 2005 (site M05B) and 2015 (site M15B) - and one that did not undergo any management (NMB) and to compare their properties with those recorded in a plot (Control) unaffected by the 2015 wildfire...
November 10, 2017: Journal of Environmental Management
Jo Ledingham, Nicola Gullick, Katherine Irving, Rachel Gorodkin, Melissa Aris, Jean Burke, Patrick Gordon, Dimitrios Christidis, Sarah Galloway, Eranga Hayes, Andrew Jeffries, Scott Mercer, Janice Mooney, Sander van Leuven, James Galloway
No abstract text is available yet for this article.
December 1, 2017: Rheumatology
Jordyn Bergsveinson, Barry Ziola
Beer-spoilage-related lactic acid bacteria (BSR LAB) belong to multiple genera and species; however, beer-spoilage capacity is isolate-specific and partially acquired via horizontal gene transfer within the brewing environment. Thus, the extent to which genus-, species-, or environment- (i.e., brewery-) level genetic variability influences beer-spoilage phenotype is unknown. Publicly available Lactobacillus brevis genomes were analyzed via BlAst Diagnostic Gene findEr (BADGE) for BSR genes and assessed for pangenomic relationships...
October 4, 2017: Canadian Journal of Microbiology
Ekaterina Mikhaylova, Valerio Tabacchini, Giacomo Borghi, Pieter Mollet, Ester D'Hoe, Dennis R Schaart, Stefaan Vandenberghe
The goal of this simulation study is the performance evaluation and comparison of six potential designs for a time-of-flight PET scanner for pediatric patients of up to about 12 years of age. It is designed to have a high sensitivity and provide high-contrast and high-resolution images. The simulated pediatric PET is a full ring scanner, consisting of 32  ×  32 mm(2) monolithic LYSO:Ce crystals coupled to digital silicon photomultiplier arrays. The six considered designs differ in axial lengths (27...
October 19, 2017: Physics in Medicine and Biology
Dichen Wang, Haoming Fan, Xiangguo Fan
Gully erosion is an important environmental problem worldwide and the main process by which water and soil losses occur in the Black Soil Region (BSR) of Northeast China. At the end of 2012, 295,663 gullies were present in this region. However, few studies have examined the gullies of the Black Soil Region as a whole. Studying the distribution of recent gullies can reveal the pattern of gully distribution and can help predict their spatial development according to the soil and water conservation regionalization of China...
September 17, 2017: Environmental Monitoring and Assessment
Lorena Casadonte, Bart-Jan Verhoeff, Jan J Piek, Ed VanBavel, Jos A E Spaan, Maria Siebes
Baseline assessment of functional stenosis severity has been proposed as a practical alternative to hyperemic indices. However, intact autoregulation mechanisms may affect intracoronary hemodynamics. The aim of this study was to investigate the effect of changes in aortic pressure (Pa) and heart rate (HR) on baseline coronary hemodynamics and functional stenosis assessment. In 15 patients (55 ± 3% diameter stenosis) Pa, intracoronary pressure (Pd) and flow velocity were obtained at control, and during atrial pacing at 120 bpm, increased Pa (+30 mmHg) with intravenous phenylephrine (PE), and elevated Pa while pacing at sinus heart rate (PE + sHR)...
September 13, 2017: Basic Research in Cardiology
Zhen Huang, Gary Peng, Xunjia Liu, Abhinandan Deora, Kevin C Falk, Bruce D Gossen, Mary R McDonald, Fengqun Yu
Clubroot, caused by Plasmodiophora brassicae, is an important disease of canola (Brassica napus) in western Canada and worldwide. In this study, a clubroot resistance gene (Rcr2) was identified and fine mapped in Chinese cabbage cv. "Jazz" using single-nucleotide polymorphisms (SNP) markers identified from bulked segregant RNA sequencing (BSR-Seq) and molecular markers were developed for use in marker assisted selection. In total, 203.9 million raw reads were generated from one pooled resistant (R) and one pooled susceptible (S) sample, and >173,000 polymorphic SNP sites were identified between the R and S samples...
2017: Frontiers in Plant Science
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"