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https://www.readbyqxmd.com/read/28421278/an-efficient-method-to-enrich-for-knock-out-and-knock-in-cellular-clones-using-the-crispr-cas9-system
#1
Francesca Niccheri, Riccardo Pecori, Silvestro G Conticello
Clustered Regularly Interspaced Short Palindromic Repeats-associated protein 9 nuclease (CRISPR/Cas9) and Transcription Activator-Like Effector Nucleases (TALENs) are versatile tools for genome editing. Here we report a method to increase the frequency of Cas9-targeted cellular clones. Our method is based on a chimeric construct with a Blasticidin S Resistance gene (bsr) placed out-of-frame by a surrogate target sequence. End joining of the CRISPR/Cas9-induced double-strand break on the surrogate target can place the bsr in frame, thus providing temporary resistance to Blasticidin S: this is used to enrich for cells where Cas9 is active...
April 18, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28365881/a-comparison-between-constitutive-and-inducible-transgenic-expression-of-the-phrip-i-gene-for-broad-spectrum-resistance-against-phytopathogens-in-potato
#2
Romel Gonzales-Salazar, Bianca Cecere, Michelina Ruocco, Rosa Rao, Giandomenico Corrado
OBJECTIVES: To engineer broad spectrum resistance in potato using different expression strategies. RESULTS: The previously identified Ribosome-Inactivating Protein from Phytolacca heterotepala was expressed in potato under a constitutive or a wound-inducible promoter. Leaves and tubers of the plants constitutively expressing the transgene were resistant to Botrytis cinerea and Rhizoctonia solani, respectively. The wound-inducible promoter was useful in driving the expression upon wounding and fungal damage, and conferred resistance to B...
April 1, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28339817/bsr-and-bhpr-guideline-for-the-prescription-and-monitoring-of-non-biologic-disease-modifying-anti-rheumatic-drugs
#3
Jo Ledingham, Nicola Gullick, Katherine Irving, Rachel Gorodkin, Melissa Aris, Jean Burke, Patrick Gordon, Dimitrios Christidis, Sarah Galloway, Eranga Hayes, Andrew Jeffries, Scott Mercer, Janice Mooney, Sander van Leuven, James Galloway
No abstract text is available yet for this article.
February 27, 2017: Rheumatology
https://www.readbyqxmd.com/read/28283005/-comparison-of-maldi-tof-and-16s-rrna-methods-in-identification-of-viridans-group-streptococci
#4
Serap Süzük Yıldız, Banu Kaşkatepe, Salih Altınok, Mustafa Çetin, Alper Karagöz, Sümeyra Savaş
Accurate identification of viridans group streptococci (VGS) frequently encountered as a causative agent of infective endocarditis is always a challenge for the clinical microbiology laboratory. Clinical microbiology laboratories generally use semi automatic/full automatic systems, molecular methods and also conventional methods for the identification of these bacteria. There are recent published studies that have used MALDI-TOF (Matrix Assisted Laser Ionization Mass Spectrometry-Time of Flight) systems in the identification of VGS...
January 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28267609/the-culicoides-sonorensis-inhibitor-of-apoptosis-1-protein-protects-mammalian-cells-from-apoptosis-induced-by-infection-with-african-horse-sickness-virus-and-bluetongue-virus
#5
Elaine Vermaak, Francois F Maree, Jacques Theron
African horse sickness virus (AHSV) and bluetongue virus (BTV) are arboviruses of the genus Orbivirus that are transmitted to their vertebrate hosts by Culicoides biting midges. These orbiviruses exhibit lytic infection (apoptosis) in mammalian cells, but cause persistent infection with no cytopathic effects in Culicoides sonorensis cells. Although regulation of apoptosis could thus be integral for establishing persistent virus infection in midge cells, nothing is known about the presence and function of apoptosis pathways in Culicoides midges and their derived cell lines...
March 4, 2017: Virus Research
https://www.readbyqxmd.com/read/28262590/the-implications-of-biologic-therapy-for-elective-foot-and-ankle-surgery-in-patients-with-rheumatoid-arthritis
#6
REVIEW
Ross Diaper, Ernest Wong, Stuart A Metcalfe
INTRODUCTION: Rheumatoid arthritis (RA) is one of a number of inflammatory arthropathies resulting in foot pain and deformity. Patients with this disease may require surgical intervention as part of their management. Many of these patients are now taking biologic agents which pose several risks to patients in the perioperative phase. The surgical team therefore need to be aware of these associated complications and how to manage these cases. AIM: This paper aims to review the current literature about perioperative needs (foot and ankle surgery) associated with patients with rheumatoid arthritis receiving biologic therapy...
March 2017: Foot
https://www.readbyqxmd.com/read/28223685/finger-posture-modulates-structural-body-representations
#7
Luigi Tamè, Elanah Dransfield, Thomas Quettier, Matthew R Longo
Patients with lesions of the left posterior parietal cortex commonly fail in identifying their fingers, a condition known as finger agnosia, yet are relatively unimpaired in sensation and skilled action. Such dissociations have traditionally been interpreted as evidence that structural body representations (BSR), such as the body structural description, are distinct from sensorimotor representations, such as the body schema. We investigated whether performance on tasks commonly used to assess finger agnosia is modulated by changes in hand posture...
February 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28214114/effects-of-salvage-logging-on-soil-properties-and-vegetation-recovery-in-a-fire-affected-mediterranean-forest-a-two-year-monitoring-research
#8
F García-Orenes, V Arcenegui, K Chrenková, J Mataix-Solera, J Moltó, A B Jara-Navarro, M P Torres
Post-fire management can have an additional impact on the ecosystem; in some cases, even more severe than the fire. Salvage logging (SL) is a common practice in most fire-affected areas. The management of burnt wood can determine microclimatic conditions and seriously affect soil properties. In some cases, the way of doing it, using heavy machinery, and the vulnerability of soils to erosion and degradation can make this management potentially aggressive to soil. Research was done in "Sierra de Mariola Natural Park" (E Spain)...
May 15, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28210929/effect-of-boswellia-serrata-resin-supplementation-on-basic-chemical-and-mineral-element-composition-in-the-muscles-and-liver-of-broiler-chickens
#9
A R M Al-Yasiry, B Kiczorowska, W Samolińska
Supplementation of broiler chicken diets with resin rich in bioactive components, such as different boswellic acids, could improve productivity, chemical composition, and nutritive value of produced meat. The aim of the study was to assess the effect of different levels of Boswellia serrata (BSR) supplementation in broiler chicken diet on the basic chemical composition and the Ca, P, Mg, Fe, Zn, and Cu contents in the breast and drumstick muscles and liver. The analyses involved 200 Ross 308 chickens. The broiler chickens were fed with diets containing 0 (BSR0), 1...
February 16, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28192287/improved-image-quality-using-monolithic-scintillator-detectors-with-dual-sided-readout-in-a-whole-body-tof-pet-ring-a-simulation-study
#10
Valerio Tabacchini, Suleman Surti, Giacomo Borghi, Joel S Karp, Dennis R Schaart
We have recently built and characterized the performance of a monolithic scintillator detector based on a 32 mm  ×  32 mm  ×  22 mm LYSO:Ce crystal read out by digital silicon photomultiplier (dSiPM) arrays coupled to the crystal front and back surfaces in a dual-sided readout (DSR) configuration. The detector spatial resolution appeared to be markedly better than that of a detector consisting of the same crystal with conventional back-sided readout (BSR). Here, we aim to evaluate the influence of this difference in the detector spatial response on the quality of reconstructed images, so as to quantify the potential benefit of the DSR approach for high-resolution, whole-body time-of-flight (TOF) positron emission tomography (PET) applications...
February 13, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28133396/a-comparative-simulation-study-of-ar-1-estimators-in-short-time-series
#11
Tanja Krone, Casper J Albers, Marieke E Timmerman
Various estimators of the autoregressive model exist. We compare their performance in estimating the autocorrelation in short time series. In Study 1, under correct model specification, we compare the frequentist r1 estimator, C-statistic, ordinary least squares estimator (OLS) and maximum likelihood estimator (MLE), and a Bayesian method, considering flat (Bf) and symmetrized reference (Bsr) priors. In a completely crossed experimental design we vary lengths of time series (i.e., T = 10, 25, 40, 50 and 100) and autocorrelation (from -0...
2017: Quality & Quantity
https://www.readbyqxmd.com/read/28088339/evidence-based-management-of-systemic-sclerosis-navigating-recommendations-and-guidelines
#12
REVIEW
Russell Edward Pellar, Janet Elizabeth Pope
OBJECTIVES: Systemic sclerosis (SSc) is a rare heterogeneous connective tissue disease. Recommendations addressing the major issues in the management of SSc including screening and treatment of organ complications are needed. METHODS: The updated European League Against Rheumatism/European Scleroderma Trial and Research (EULAR/EUSTAR) and the British Society of Rheumatology (BSR) and British Health Professionals in Rheumatology (BHPR) guidelines were compared and contrasted...
December 9, 2016: Seminars in Arthritis and Rheumatism
https://www.readbyqxmd.com/read/28060816/prediction-of-hexaconazole-concentration-in-the-top-most-layer-of-oil-palm-plantation-soil-using-exploratory-data-analysis-eda
#13
Zainol Maznah, Muhamad Halimah, Mahendran Shitan, Provash Kumar Karmokar, Sulaiman Najwa
Ganoderma boninense is a fungus that can affect oil palm trees and cause a serious disease called the basal stem root (BSR). This disease causes the death of more than 80% of oil palm trees midway through their economic life and hexaconazole is one of the particular fungicides that can control this fungus. Hexaconazole can be applied by the soil drenching method and it will be of interest to know the concentration of the residue in the soil after treatment with respect to time. Hence, a field study was conducted in order to determine the actual concentration of hexaconazole in soil...
2017: PloS One
https://www.readbyqxmd.com/read/27918241/detection-of-oil-palm-root-penetration-by-agrobacterium-mediated-transformed-ganoderma-boninense-expressing-green-fluorescent-protein
#14
Nisha Govender, Mui-Yun Wong
A highly efficient and reproducible Agrobacterium-mediated transformation protocol for Ganoderma boninense was developed to facilitate observation of the early stage infection of basal stem rot (BSR). The method was proven amenable to different explants (basidiospore, protoplast, and mycelium) of G. boninense. The transformation efficiency was highest (62%) under a treatment combination of protoplast explant and Agrobacterium strain LBA4404, with successful expression of an hyg marker gene and gus-gfp fusion gene under the control of heterologous p416 glyceraldehyde 3-phosphate dehydrogenase promoter...
April 2017: Phytopathology
https://www.readbyqxmd.com/read/27902793/snp-discovery-and-qtl-mapping-of-sclerotinia-basal-stalk-rot-resistance-in-sunflower-using-genotyping-by-sequencing
#15
Zahirul I Talukder, Gerald J Seiler, Qijian Song, Guojia Ma, Lili Qi
Basal stalk rot (BSR), caused by the ascomycete fungus (Lib.) de Bary, is a serious disease of sunflower ( L.) in the cool and humid production areas of the world. Quantitative trait loci (QTL) for BSR resistance were identified in a sunflower recombinant inbred line (RIL) population derived from the cross HA 441 × RHA 439. A genotyping-by-sequencing (GBS) approach was adapted to discover single nucleotide polymorphism (SNP) markers. A genetic linkage map was developed comprised of 1053 SNP markers on 17 linkage groups (LGs) spanning 1401...
November 2016: Plant Genome
https://www.readbyqxmd.com/read/27898824/genome-wide-association-study-of-brown-stem-rot-resistance-in-soybean-across-multiple-populations
#16
Keith Rincker, Alexander E Lipka, Brian W Diers
Genetic resistance to brown stem rot (BSR) of soybean [ (L.) Merr.], caused by (Allington & D.W. Chamb.) T.C. Harr. & McNew, has been identified and mapped with biparental populations. Although nearly 400 accessions have been identified with BSR resistance, this trait has been mapped in only 12 sources, and just two, PI84946-2 and PI88788, have been used to develop BSR resistant cultivars. Thus, there is a serious need to improve our knowledge of the genetic basis of BSR resistance in soybean so that resistance genes in cultivars can be diversified and markers close to resistance genes can be identified and used in marker-assisted selection (MAS)...
July 2016: Plant Genome
https://www.readbyqxmd.com/read/27898763/fine-mapping-of-resistance-genes-from-five-brown-stem-rot-resistance-sources-in-soybean
#17
Keith Rincker, Glen L Hartman, Brian W Diers
Brown stem rot (BSR) of soybean [ (L.) Merr.] caused by (Allington & Chamb.) T.C. Harr. & McNew can be controlled effectively with genetic host resistance. Three BSR resistance genes , , and , have been identified and mapped to a large region on chromosome 16. Marker-assisted selection (MAS) will be more efficient and gene cloning will be facilitated with a narrowed genomic interval containing an gene. The objective of this study was to fine map the positions of genes from five sources. Mapping populations were developed by crossing the resistant sources 'Bell', PI 84946-2, PI 437833, PI 437970, L84-5873, and PI 86150 with either the susceptible cultivar Colfax or Century 84...
March 2016: Plant Genome
https://www.readbyqxmd.com/read/27888533/advances-in-understanding-broad-spectrum-resistance-to-pathogens-in-rice
#18
Yinggen Ke, Hanqing Deng, Shiping Wang
Rice diseases caused by multiple pathogen species are a major obstacle to achieving optimal yield. Using host pathogen species-nonspecific broad-spectrum resistance (BSR) for rice improvement is an efficient way to control diseases. Recent advances in rice genomics and improved understanding of the mechanisms of rice-pathogen interactions have shown that using a single gene to improve rice BSR to multiple pathogen species is technically possible and the necessary resources exist. A variety of rice genes, including major disease resistance genes and defense-responsive genes, which function in pattern-triggered immunity signaling, effector-triggered immunity signaling, or quantitative resistance, can mediate BSR to two or more pathogen species independently...
November 26, 2016: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/27867719/monitoring-cerebral-oxygenation-and-local-field-potential-with-a-variation-of-isoflurane-concentration-in-a-rat-model
#19
Dong-Hyuk Choi, Teo Jeon Shin, Seonghyun Kim, Jayyoung Bae, Dongrae Cho, Jinsil Ham, Ji-Young Park, Hyoung-Ihl Kim, Seongwook Jeong, Boreom Lee, Jae Gwan Kim
We aimed to investigate experimentally how anesthetic levels affect cerebral metabolism measured by near-infrared spectroscopy (NIRS) and to identify a robust marker among NIRS parameters to discriminate various stages of anesthetic depth in rats under isoflurane anesthesia. In order to record the hemodynamic changes and local field potential (LFP) in the brain, fiber-optic cannulae and custom-made microelectrodes were implanted in the frontal cortex of the skull. The NIRS and LFP signals were continuously monitored before, during and after isoflurane anesthesia...
October 1, 2016: Biomedical Optics Express
https://www.readbyqxmd.com/read/27848024/-current-guidelines-on-anca-associated-vasculitides-common-features-and-differences
#20
B Hellmich
The results of a number of prospective randomized controlled clinical trials have led to changes in established strategies for the treatment of antineutrophil cytoplasmic antibody (ANCA) associated vasculitides (AAV) in recent years. Since 2014, a total of 4 scientific societies and study groups have systematically reviewed the new data and have formulated evidence-based recommendations for the management of AAV based on the analysis. These recommendations contain information on diagnosis, treatment (induction and maintenance), supportive care and monitoring of disease activity and resulting damage...
November 15, 2016: Zeitschrift Für Rheumatologie
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