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https://www.readbyqxmd.com/read/27918241/detection-of-oil-palm-root-penetration-by-agrobacterium-mediated-transformed-ganoderma-boninense-expressing-green-fluorescent-protein
#1
Nisha Thopla Govender, Mui-Yun Wong
A highly efficient and reproducible Agrobacterium-mediated transformation protocol for Ganoderma boninense was developed to facilitate observation of the early stage infection of basal stem rot (BSR). The method was proven amenable to different explants (basidiospore, protoplast and mycelium) of G. boninense. The transformation efficiency was highest (62%) under a treatment combination of protoplast explants and Agrobacterium strain LBA 4404 with successful expression of hygromycin (hyg) marker gene and gus-gfp fusion gene, under the control of heterologous p416 gpd promoter...
December 5, 2016: Phytopathology
https://www.readbyqxmd.com/read/27902793/snp-discovery-and-qtl-mapping-of-sclerotinia-basal-stalk-rot-resistance-in-sunflower-using-genotyping-by-sequencing
#2
Zahirul I Talukder, Gerald J Seiler, Qijian Song, Guojia Ma, Lili Qi
Basal stalk rot (BSR), caused by the ascomycete fungus (Lib.) de Bary, is a serious disease of sunflower ( L.) in the cool and humid production areas of the world. Quantitative trait loci (QTL) for BSR resistance were identified in a sunflower recombinant inbred line (RIL) population derived from the cross HA 441 × RHA 439. A genotyping-by-sequencing (GBS) approach was adapted to discover single nucleotide polymorphism (SNP) markers. A genetic linkage map was developed comprised of 1053 SNP markers on 17 linkage groups (LGs) spanning 1401...
November 2016: Plant Genome
https://www.readbyqxmd.com/read/27898824/genome-wide-association-study-of-brown-stem-rot-resistance-in-soybean-across-multiple-populations
#3
Keith Rincker, Alexander E Lipka, Brian W Diers
Genetic resistance to brown stem rot (BSR) of soybean [ (L.) Merr.], caused by (Allington & D.W. Chamb.) T.C. Harr. & McNew, has been identified and mapped with biparental populations. Although nearly 400 accessions have been identified with BSR resistance, this trait has been mapped in only 12 sources, and just two, PI84946-2 and PI88788, have been used to develop BSR resistant cultivars. Thus, there is a serious need to improve our knowledge of the genetic basis of BSR resistance in soybean so that resistance genes in cultivars can be diversified and markers close to resistance genes can be identified and used in marker-assisted selection (MAS)...
July 2016: Plant Genome
https://www.readbyqxmd.com/read/27898763/fine-mapping-of-resistance-genes-from-five-brown-stem-rot-resistance-sources-in-soybean
#4
Keith Rincker, Glen L Hartman, Brian W Diers
Brown stem rot (BSR) of soybean [ (L.) Merr.] caused by (Allington & Chamb.) T.C. Harr. & McNew can be controlled effectively with genetic host resistance. Three BSR resistance genes , , and , have been identified and mapped to a large region on chromosome 16. Marker-assisted selection (MAS) will be more efficient and gene cloning will be facilitated with a narrowed genomic interval containing an gene. The objective of this study was to fine map the positions of genes from five sources. Mapping populations were developed by crossing the resistant sources 'Bell', PI 84946-2, PI 437833, PI 437970, L84-5873, and PI 86150 with either the susceptible cultivar Colfax or Century 84...
March 2016: Plant Genome
https://www.readbyqxmd.com/read/27888533/advances-in-understanding-broad-spectrum-resistance-to-pathogens-in-rice
#5
Yinggen Ke, Hanqing Deng, Shiping Wang
Rice diseases caused by multiple pathogen species are a major obstacle to achieving optimal yield. Using host pathogen species-nonspecific broad-spectrum resistance (BSR) for rice improvement is an efficient way to control diseases. Recent advances in rice genomics and improved understanding of the mechanisms of rice-pathogen interactions have shown that using a single gene to improve rice BSR to multiple pathogen species is technically possible and the necessary resources exist. A variety of rice genes, including major disease resistance genes and defense-responsive genes, which function in pattern-triggered immunity signaling, effector-triggered immunity signaling, or quantitative resistance, can mediate BSR to two or more pathogen species independently...
November 26, 2016: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/27867719/monitoring-cerebral-oxygenation-and-local-field-potential-with-a-variation-of-isoflurane-concentration-in-a-rat-model
#6
Dong-Hyuk Choi, Teo Jeon Shin, Seonghyun Kim, Jayyoung Bae, Dongrae Cho, Jinsil Ham, Ji-Young Park, Hyoung-Ihl Kim, Seongwook Jeong, Boreom Lee, Jae Gwan Kim
We aimed to investigate experimentally how anesthetic levels affect cerebral metabolism measured by near-infrared spectroscopy (NIRS) and to identify a robust marker among NIRS parameters to discriminate various stages of anesthetic depth in rats under isoflurane anesthesia. In order to record the hemodynamic changes and local field potential (LFP) in the brain, fiber-optic cannulae and custom-made microelectrodes were implanted in the frontal cortex of the skull. The NIRS and LFP signals were continuously monitored before, during and after isoflurane anesthesia...
October 1, 2016: Biomedical Optics Express
https://www.readbyqxmd.com/read/27848024/-current-guidelines-on-anca-associated-vasculitides-common-features-and-differences
#7
B Hellmich
The results of a number of prospective randomized controlled clinical trials have led to changes in established strategies for the treatment of antineutrophil cytoplasmic antibody (ANCA) associated vasculitides (AAV) in recent years. Since 2014, a total of 4 scientific societies and study groups have systematically reviewed the new data and have formulated evidence-based recommendations for the management of AAV based on the analysis. These recommendations contain information on diagnosis, treatment (induction and maintenance), supportive care and monitoring of disease activity and resulting damage...
November 15, 2016: Zeitschrift Für Rheumatologie
https://www.readbyqxmd.com/read/27842488/genetic-architecture-of-the-maize-kernel-row-number-revealed-by-combining-qtl-mapping-using-a-high-density-genetic-map-and-bulked-segregant-rna-sequencing
#8
Changlin Liu, Qiang Zhou, Le Dong, Hui Wang, Fang Liu, Jianfeng Weng, Xinhai Li, Chuanxiao Xie
BACKGROUND: The maize kernel row number (KRN) is a key component that contributes to grain yield and has high broad-sense heritability (H (2) ). Quantitative trait locus/loci (QTL) mapping using a high-density genetic map is a powerful approach to detecting loci that are responsible for traits of interest. Bulked segregant ribonucleic acid (RNA) sequencing (BSR-seq) is another rapid and cost-effective strategy to identify QTL. Combining QTL mapping using a high-density genetic map and BSR-seq may dissect comprehensively the genetic architecture underlying the maize KRN...
November 14, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27823860/contributions-and-source-identification-of-biogenic-and-anthropogenic-hydrocarbons-to-secondary-organic-aerosols-at-mt-tai-in-2014
#9
Yanhong Zhu, Lingxiao Yang, Kimitaka Kawamura, Jianmin Chen, Kaori Ono, Xinfeng Wang, Likun Xue, Wenxing Wang
Ambient fine particulate matter (PM2.5) and volatile organic compounds (VOCs) collected at Mt. Tai in summer 2014 were analysed and the data were used to identify the contribution of biogenic and anthropogenic hydrocarbons to secondary organic aerosols (SOA) and their sources and potential source areas in high mountain regions. Compared with those in 2006, the 2014 anthropogenic SOA tracers in PM2.5 aerosols and VOC species related to vehicular emissions exhibited higher concentrations, whereas the levels of biogenic SOA tracers were lower, possibly due to decreased biomass burning...
January 2017: Environmental Pollution
https://www.readbyqxmd.com/read/27797497/sulfur-cycling-related-biogeochemical-processes-of-arsenic-mobilization-in-the-western-hetao-basin-china-evidence-from-multiple-isotope-approaches
#10
Huaming Guo, Yinzhu Zhou, Yongfeng Jia, Xiaohui Tang, Xiaofeng Li, Mengmeng Shen, Hai Lu, Shuangbao Han, Chao Wei, Stefan Norra, Fucun Zhang
The role of sulfur cycling in arsenic behavior under reducing conditions is not well-understood in previous investigations. This study provides observations of sulfur and oxygen isotope fractionation in sulfate and evaluation of sulfur cycling-related biogeochemical processes controlling dissolved arsenic groundwater concentrations using multiple isotope approaches. As a typical basin hosting high arsenic groundwater, the western Hetao basin was selected as the study area. Results showed that, along the groundwater flow paths, groundwater δ34SSO4, δ18OSO4 and δ13CDOC increased with increases in arsenic, dissolved iron, hydrogen sulfide and ammonium concentrations, while δ13CDIC decreased with decreasing Eh and sulfate/chloride...
October 31, 2016: Environmental Science & Technology
https://www.readbyqxmd.com/read/27796927/an-in-vitro-study-of-the-antifungal-activity-of-trichoderma-virens-7b-and-a-profile-of-its-non-polar-antifungal-components-released-against-ganoderma-boninense
#11
Lee Pei Lee Angel, Mohd Termizi Yusof, Intan Safinar Ismail, Bonnie Tay Yen Ping, Intan Nur Ainni Mohamed Azni, Norman Hj Kamarudin, Shamala Sundram
Ganoderma boninense is the causal agent of a devastating disease affecting oil palm in Southeast Asian countries. Basal stem rot (BSR) disease slowly rots the base of palms, which radically reduces productive lifespan of this lucrative crop. Previous reports have indicated the successful use of Trichoderma as biological control agent (BCA) against G. boninense and isolate T. virens 7b was selected based on its initial screening. This study attempts to decipher the mechanisms responsible for the inhibition of G...
November 2016: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/27745113/passive-bioremediation-technology-incorporating-lignocellulosic-spent-mushroom-compost-and-limestone-for-metal-and-sulfate-rich-acid-mine-drainage
#12
Siti Nurjaliah Muhammad, Faradiella Mohd Kusin, Mohd Syakirin Md Zahar, Ferdaus Mohamat Yusuff, Normala Halimoon
Passive bioremediation of metal- and sulfate-containing acid mine drainage (AMD) has been investigated in a batch study. Multiple substrates were used in the AMD remediation using spent mushroom compost (SMC), limestone, activated sludge (AS), and woodchips (WC) under anoxic conditions suitable for bacterial sulfate reduction (BSR). Limestones used were of crushed limestone (CLS) and uncrushed limestone, provided at two different ratios in mixed substrates treatment and varied by the proportion of SMC and limestone...
October 17, 2016: Environmental Technology
https://www.readbyqxmd.com/read/27721689/control-of-basal-stem-rot-disease-in-oil-palm-by-supplementation-of-calcium-copper-and-salicylic-acid
#13
M Shahul Hamid Rahamah Bivi, Adamu Saidu Paiko, Ahmad Khairulmazmi, M S Akhtar, Abu Seman Idris
Continuous supplementation of mineral nutrients and salicylic acid (SA) as foliar application could improve efficacy in controlling basal stem rot (BSR) disease in oil palm seedling. It is revealed from the results that the highest disease severity index (58.3%) was recorded in T8 treatments at 9 months after inoculation. The best disease control was achieved by T7 treatments (calcium/copper/SA [Ca/Cu/SA]) (5.0%) followed by T1 (5.5%), T5 (5.8%), T3 (8.3%), T6 (8.3%), T4 (13.3%), and T2 (15.8%) treatments. Continuous supplementation of Ca/Cu/SA was found to be the most effective in controlling the disease and the high performance liquid chromatography results showed the detection of ergosterol at very low concentration in the treated samples...
October 2016: Plant Pathology Journal
https://www.readbyqxmd.com/read/27708345/characterization-of-maize-roothairless6-which-encodes-a-d-type-cellulose-synthase-and-controls-the-switch-from-bulge-formation-to-tip-growth
#14
Li Li, Stefan Hey, Sanzhen Liu, Qiang Liu, Colton McNinch, Heng-Cheng Hu, Tsui-Jung Wen, Caroline Marcon, Anja Paschold, Wesley Bruce, Patrick S Schnable, Frank Hochholdinger
Root hairs are tubular extensions of the epidermis. Root hairs of the monogenic recessive maize mutant roothairless 6 (rth6) are arrested after bulge formation during the transition to tip growth and display a rough cell surface. BSR-Seq in combination with Seq-walking and subsequent analyses of four independently generated mutant alleles established that rth6 encodes CSLD5 a plasma membrane localized 129 kD D-type cellulose synthase with eight transmembrane domains. Cellulose synthases are required for the biosynthesis of cellulose, the most abundant biopolymer of plant cell walls...
October 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27628445/bronchial-stump-recurrence-after-complete-resection-of-lung-cancer-effect-of-the-distance-between-the-tumor-and-bronchial-resection-margin
#15
Mehmet Zeki Gunluoglu, Celalettin Ibrahim Kocaturk, Adalet Demir, Halide Nur Urer, Seyyit Ibrahim Dincer, Mehmet Ali Bedirhan
Purpose The purpose of this study was to evaluate the following parameters after complete resection in established lung cancer patients: the frequency of bronchial stump recurrence (BSR), the effect of the distance between the tumor and bronchial resection margin (DBTM) on BSR, the survival of patients with BSR, and the effect of the DBTM on survival. Patients and Methods We retrospectively evaluated 553 consecutive lung cancer patients who underwent complete lung resection. The patients were classified as DBTM: ≤ 10 mm (group 1), 11 to 20 mm (group 2), and > 20 mm (group 3)...
September 13, 2016: Thoracic and Cardiovascular Surgeon
https://www.readbyqxmd.com/read/27616984/ventral-midbrain-nmda-receptor-blockade-from-enhanced-reward-and-dopamine-inactivation
#16
Giovanni Hernandez, Marie-Pierre Cossette, Peter Shizgal, Pierre-Paul Rompré
Glutamate stimulates ventral midbrain (VM) N-Methyl-D-Aspartate receptors (NMDAR) to initiate dopamine (DA) burst firing activity, a mode of discharge associated with enhanced DA release and reward. Blockade of VM NMDAR, however, enhances brain stimulation reward (BSR), the results can be explained by a reduction in the inhibitory drive on DA neurons that is also under the control of glutamate. In this study, we used fast-scan cyclic voltammetry (FSCV) in anesthetized animals to determine whether this enhancement is associated with a change in phasic DA release in the nucleus accumbens...
2016: Frontiers in Behavioral Neuroscience
https://www.readbyqxmd.com/read/27585859/chronic-recurrent-multifocal-osteomyelitis-in-association-with-pyoderma-gangraenosum
#17
Matthias Christian Wurm, Ines Brecht, Michael Lell, Kathrin Brunner, Konstantinos Theodorou Mitsimponas, Martin Chada, Julia Jahn, Friedrich-Wilhelm Neukam, Cornelius von Wilmowsky
BACKGROUND: Chronic recurrent multifocal osteomyelitis (CRMO) is a rare acquired inflammatory skeletal disorder of unknown origin. CRMO was first described by Gideon in 1972 and mainly affects children and young adults of female gender. The CRMO is part of the clinical picture of non-bacterial Osteomyelitis (NBO) and typically presents a relapsing recurring course with both remission and spontaneous exacerbation. CRMO is typically encountered in the limbs and the metaphysis of long bones in particular...
2016: BMC Oral Health
https://www.readbyqxmd.com/read/27558584/bsr-and-bhpr-guideline-for-the-treatment-of-axial-spondyloarthritis-including-ankylosing-spondylitis-with-biologics
#18
REVIEW
Louise Hamilton, Nick Barkham, Ashok Bhalla, Robin Brittain, Debbie Cook, Gareth Jones, Kirsten Mackay, David Marshall, Helena Marzo-Ortega, Daniel Murphy, Claire Riddell, Raj Sengupta, Stefan Siebert, Liz Van Rossen, Karl Gaffney
No abstract text is available yet for this article.
August 24, 2016: Rheumatology
https://www.readbyqxmd.com/read/27497184/african-horse-sickness-virus-infects-bsr-cells-through-macropinocytosis
#19
Elaine Vermaak, Andelé M Conradie, Francois F Maree, Jacques Theron
Cellular pathways involved in cell entry by African horse sickness virus (AHSV), a member of the Orbivirus genus within the Reoviridae family, have not yet been determined. Here, we show that acidic pH is required for productive infection of BSR cells by AHSV-4, suggesting that the virus is likely internalized by an endocytic pathway. We subsequently analyzed the major endocytic routes using specific inhibitors and determined the consequences for AHSV-4 entry into BSR cells. The results indicated that virus entry is dynamin dependent, but clathrin- and lipid raft/caveolae-mediated endocytic pathways were not used by AHSV-4 to enter and infect BSR cells...
October 2016: Virology
https://www.readbyqxmd.com/read/27477701/split-belt-locomotion-in-parkinson-s-disease-links-asymmetry-dyscoordination-and-sequence-effect
#20
Alfonso Fasano, Christian Schlenstedt, Jan Herzog, Meir Plotnik, Franziska E M Rose, Jens Volkmann, Günther Deuschl
BACKGROUND: The pathophysiology behind gait impairments seen in Parkinson's disease (PD), in particular freezing of gait (FOG), is not fully understood. Here we study the interplay between several gait features related to FOG during different split-belt treadmill (SBTM) conditions. METHODS: We investigated the spatiotemporal properties, the phenomenon of sequence effect and the inter-limb symmetry and temporal coordination of gait during different split-belt conditions in 20 patients with advanced Parkinson's disease and different severities of freezing...
July 2016: Gait & Posture
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