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https://www.readbyqxmd.com/read/29353456/in-vivo-transient-and-partial-cell-reprogramming-to-pluripotency-as-a-therapeutic-tool-for-neurodegenerative-diseases
#1
REVIEW
S Tamanini, G P Comi, S Corti
In theory, human diseases in which a specific cell type degenerates, such as neurodegenerative diseases, can be therapeutically addressed by replacement of the lost cells. The classical strategy for cell replacement is exogenous cell transplantation, but now, cell replacement can also be achieved with in situ reprogramming. Indeed, many of these disorders are age-dependent, and "rejuvenating" strategies based on cell epigenetic modifications are a possible approach to counteract disease progression. In this context, transient and/or partial reprogramming of adult somatic cells towards pluripotency can be a promising tool for neuroregeneration...
January 20, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29353415/investigation-of-lactic-acid-bacteria-isolated-from-giant-panda-feces-for-potential-probiotics-in-vitro
#2
Qian Liu, Xueqin Ni, Qiang Wang, Zhirong Peng, Lili Niu, Meiling Xie, Yicen Lin, Yi Zhou, Hao Sun, Kangcheng Pan, Bo Jing, Dong Zeng
The present study aimed to isolate an optimal lactic acid bacterial strain from the feces of healthy giant pandas. The strain exhibited good stability at low pH and high bile salt concentrations, activity against pathogens relevant to pandas, and antibiotic susceptibility. In the current study, 25 isolates were obtained from de Man, Rogosa, and Sharpe agar. Two (E21 and G83) and eight (E1, E2, E16, E18, E21, E69, E70, and G83) isolates demonstrated good performance at pH 2.0 and bile 2% (w/v), respectively...
January 20, 2018: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29353230/a-model-of-dormant-emergent-metastatic-breast-cancer-progression-enabling-exploration-of-biomarker-signatures
#3
Amanda M Clark, Manu Kumar, Sarah Wheeler, Carissa Young, Raman Venkataramanan, Donna Stolz, Linda Griffith, Douglas A Lauffenburger, Alan Wells
Breast cancer mortality predominantly results from dormant micrometastases that emerge as fatal outgrowths years after initial diagnosis. In order to gain insights concerning factors associated with emergence of liver metastases, we recreated spontaneous dormancy in an all-human ex vivo hepatic microphysiological system (MPS). Seeding this MPS with small numbers (<0.05% by cell count) of the aggressive MDA-MB-231 breast cancer cell line, two populations formed: actively proliferating ('growing'; EdU+), and spontaneously quiescent ('dormant'; EdU-)...
January 20, 2018: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29353209/mir-182-promotes-prostate-cancer-progression-through-activating-wnt-%C3%AE-catenin-signal-pathway
#4
Dawei Wang, Guoliang Lu, Yuan Shao, Da Xu
Although prostate cancer can be surgical excised and effectively treated by androgen-deprivation therapy, radiotherapy, or chemotherapy, management of patients with advanced or drug-resistance prostate cancer stills remains a big trouble. Accumulated evidence indicated that miR-182 and Wnt/β-catenin function as tumor oncogene in the progression of a variety of tumors. However, little is known about how miR-182 regulates β-catenin signal molecular and impacts on the tumorigenesis of human prostate cancer. In this study, employing the analyses of qRT-PCR, we found that prostate cancer tissues expressed much more miR-182 than non-cancer tissues did...
January 17, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29353203/the-water-extract-of-tutsan-hypericum-androsaemum-l-red-berries-exerts-antidepressive-like-effects-and-in-vivo-antioxidant-activity-in-a-mouse-model-of-post-stroke-depression
#5
Seyed Mohammad Nabavi, Seyed Fazel Nabavi, Antoni Sureda, Giovanni Caprioli, Romilde Iannarelli, Arold Jorel Tsetegho Sokeng, Nady Braidy, Sedigheh Khanjani, Akbar Hajizadeh Moghaddam, Atanas G Atanasov, Maria Daglia, Filippo Maggi
Hypericum androsaemum L., commonly known as 'tutsan' or 'shrubby St. John's Wort', is a member of the Hypericum genus found growing spontaneously in the Mediterranean area and is cultivated extensively as an ornamental plant due to the showy color variation in its fresh berry-like capsules, which turn from red to shiny black as they ripen. Tutsan has also been used in Portuguese and Spanish folk medicine to treat depression. In this study, we assessed the beneficial role of the water extract of H. androsaemum red berries (WE) in an experimental animal model of post-stroke depression...
January 16, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29353143/contractile-effect-of-rifaximin-on-bovine-uterus-in-the-presence-of-steroid-hormone-antagonists
#6
R L Sciorsci, M Piccinno, A Rizzo
This in vitro study investigated the modulatory effect of rifaximin on bovine uterus contractility, in both phases of the oestrous cycle, with and without the steroid hormones that are predominant in the respective phases: oestrogen in the follicular phase and progesterone in the luteal phase. The procedures were conducted in an isolated organ bath by using rifaximin alone (10-4 M) and in association with the steroid hormone antagonists (10-5 M) tamoxifen (oestrogen antagonist) in the follicular phase and mifepristone (progesterone antagonist) in the luteal phase...
January 10, 2018: Theriogenology
https://www.readbyqxmd.com/read/29353142/mirna-185-regulates-the-vegfa-signaling-pathway-in-dairy-cows-with-retained-fetal-membranes
#7
REVIEW
C Y Zheng, X Zou, H J Lin, B C Zhao, M L Zhang, C H Luo, S X Fu
Retention of fetal membranes (RFM) of cows is an important reproductive disturbance, and is related to miRNAs. Vascular endothelial growth factor (VEGF)A, regulated by miRNA-185, can activate arachidonic acid (ARA) release via the VEGFA signaling pathway, which influences RFM. The aim of this study was to explore the pathogenic mechanism of RFM by investigating the regulatory relationship between miRNA-185 and the VEGFA signaling pathway. Serum samples of healthy Holstein dairy cows (n = 20) and RFM cows (n = 12), with a similar age, parity, weight, and milk yield, were collected to detect VEGFA and ARA concentrations at 6, 12, and 24 h after calving...
January 9, 2018: Theriogenology
https://www.readbyqxmd.com/read/29353104/galactosylated-iodine-based-small-molecule-i-v-ct-contrast-agent-for-bile-duct-imaging
#8
Yeonjin Jung, Hee Sook Hwang, Kun Na
Computed tomography (CT) with contrast plays an important role as a clinical diagnostic tool but still has a limited diagnostic range. In this work, we developed a novel injectable iodine-based small molecule CT contrast agent, even can be used for bile duct diagnostics. The bile duct diagnosable CT contrast agent (BDICA) is synthesized with 5-amino-2,4,6-triiodoisophthaloyl dichloride (ATIPC), tromethamine and lactobionic acid (LBA) for asialoglycoprotein receptor (ASGPR) targeted delivery via receptor-mediated endocytosis and transport to the bile canaliculi...
January 12, 2018: Biomaterials
https://www.readbyqxmd.com/read/29353068/mitochondrial-atp-sensitive-potassium-channel-regulates-mitochondrial-dynamics-to-participate-in-neurodegeneration-of-parkinson-s-disease
#9
Kaige Peng, Jun Hu, Jingsong Xiao, Guorong Dan, Likui Yang, Feng Ye, Zhongmin Zou, Jia Cao, Yan Sai
Parkinson's disease (PD) is the second most common age-related neurodegenerative disease. Mitochondrial dysfunction has been the focus of the pathogenesis of PD. The mitochondrial ATP-sensitive potassium channel (mitoKATP) plays a significant role in mitochondrial physiology and has been extensively shown to protect against ischemic and brain reperfusion injury. However, there have long been controversies regarding its role in Parkinson's disease. We investigated the role of mitoKATP channels in rotenone-induced PD model in vivo and vitro and the interactions of mitoKATP channels, mitochondrial dynamics and PD...
January 15, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29353059/ask-family-kinases-are-required-for-optimal-nlrp3-inflammasome-priming
#10
David E Place, Parimal Samir, Rajendra Karki, Benoit Briard, Peter Vogel, Thirumala-Devi Kanneganti
Activation of the multimeric inflammasome complex leads to inflammatory responses to biotic and abiotic triggers. The inflammasome sensor, Nod-like receptor family Pyrin domain containing 3 (NLRP3), is activated by a range of stimuli and is tightly regulated to restrict excessive inflammation. Because NLRP3 responds broadly to cellular insults and regulates cell death similar to the stress-activated apoptosis signal-regulating kinases 1 and 2 (ASK1/2), we hypothesized that ASK1/2 may regulate NLRP3 activity...
January 15, 2018: American Journal of Pathology
https://www.readbyqxmd.com/read/29353042/ginkgolic-acid-induces-interplay-between-apoptosis-and-autophagy-regulated-by-ros-generation-in-colon-cancer
#11
Yuxia Liu, Bin Yang, Lirong Zhang, Xianling Cong, Zhen Liu, Yu Hu, Jing Zhang, Haixia Hu
Presently, developing effective anti-colon cancer drugs still remains to be important. Ginkgolic acids (GA), as a botanical drug extracted from the seed coat of Ginkgo biloba L., possess various bioactive properties. Our findings, for the first time, indicated that GA suppressed colon cancer cell proliferation, migration and invasion. GA led to cell death through G0/G1 phase arrest. In addition, apoptosis was significantly induced by GA treatment. The intrinsic apoptosis pathway was included, proved by the release of cytochrome c (Cyto-c) from the mitochondria into the cytosol...
January 15, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29353038/cereblon-crbn-deletion-reverses-streptozotocin-induced-diabetic-osteoporosis-in-mice
#12
Cong Yao, Xiong Guo, Wan-Xia Yao, Chun Zhang
Diabetes mellitus is a major cause to induce osteoporosis. Though the pathogenesis of osteoporosis progression has been well investigated, its still not fully understood. Recently, cereblon (CRBN) was considered as a negative modulator of adenosine monophosphate-activated protein kinase (AMPK) in vitro and in vivo. Here, we presented results indicating that CRBN could effectively regulate osteoporosis development. In STZ-induced wild type (WT) mice with diabetes, the osteoclasts were highly increased along with the deterioration of bone structure...
January 15, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29352993/comparison-of-chorioretinal-layers-in-rhesus-macaques-using-spectral-domain-optical-coherence-tomography-and-high-resolution-histological-sections
#13
Glenn Yiu, Zhe Wang, Christian Munevar, Eric Tieu, Bradley Shibata, Brittany Wong, David Cunefare, Sina Farsiu, Jeffrey Roberts, Sara Thomasy
Nonhuman primates are important preclinical models of retinal diseases because they uniquely possess a macula similar to humans. Ocular imaging technologies such as spectral-domain optical coherence tomography (SD-OCT) allow noninvasive, in vivo measurements of chorioretinal layers with near-histological resolution. However, the boundaries are based on differences in reflectivity, and detailed correlations with histological tissue layers have not been explored in rhesus macaques, which are widely used for biomedical research...
January 15, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29352982/inhibition-of-cyp3a-by-antimalarial-piperaquine-and-its-metabolites-in-human-liver-microsomes-with-iviv-extrapolation
#14
Mohd Yusmaidie Aziz, Kurt-Jürgen Hoffmann, Michael Ashton
The potential of the antimalarial piperaquine and its metabolites to inhibit CYP3A was investigated in pooled human liver microsomes. CYP3A activity was measured by LC-MS/MS as the rate of 1'-hydroxymidazolam formation. Piperaquine was found to be a reversible, potent inhibitor of CYP3A with the following parameter estimates (%CV): IC50 = 0.76 μM (29), Ki = 0.68 μM (29). In addition, piperaquine acted as a time-dependent inhibitor (TDI) with IC50 declining to 0.32 μM (28) during 30 min pre-incubation. TDI estimates were kinact = 0...
January 15, 2018: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29352974/chrysin-a-natural-and-biologically-active-flavonoid-suppresses-tumor-growth-of-mouse-b16f10-melanoma-cells-in-vitro-and-in-vivo-study
#15
Aïcha Sassi, Mouna Maatouk, Dorra El Gueder, Imen Mokdad Bzéouich, Saïda Abdelkefi-Ben Hatira, Saloua Jemni-Yacoub, Kamel Ghedira, Leila Chekir-Ghedira
Chrysin (5,7-dihydroxyflavone) is a natural and biologically active compound which has many biological activities as an anticancer agent. The current report is aimed at finding out whether the antitumor potential of chrysin, evidenced in vitro and in vivo, is linked or not to its effect on immunological mechanisms of melanoma-bearing mice. Chrysin-treated B16F10 cells were analyzed for their metabolic rate and apoptotic potentials. In vivo, BALB/c mice received a subcutaneous injection of B16F10 melanoma cells prior to antitumor treatments with chrysin (50 mg/kg b...
January 15, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29352936/freeze-dried-chitosan-acetate-dressings-with-glycosaminoglycans-and-traxenamic-acid
#16
Francesca Saporito, Giuseppina Sandri, Silvia Rossi, Maria Cristina Bonferoni, Federica Riva, Lorenzo Malavasi, Carla Caramella, Franca Ferrari
Bleeding control plays an important role to increase survival in the early phase after a traumatic event. The aim of present work was the development of hemostatic sponge-like dressings based on chitosan, in association with glycosaminoglycans (GAG) (chondroitin sulfate or hyaluronic acid) and the improvement of their hemostatic performance by loading tranexamic acid (TA). The dressings were prepared by lyophilizazion and were characterized for mechanical, hydration, bioadhesion properties and morphology. Moreover, FTIR analysis was performed to understand the interactions between the different polyelectrolytes present in the dressings...
March 15, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29352928/thermo-sensitive-injectable-glycol-chitosan-based-hydrogel-for-treatment-of-degenerative-disc-disease
#17
Zhengzheng Li, Hyeeun Shim, Myeong Ok Cho, Ik Sung Cho, Jin Hyun Lee, Sun-Woong Kang, Bosun Kwon, Kang Moo Huh
The use of injectable hydrogel formulations have been suggested as a promising strategy for the treatment of degenerative disc disease to both restore the biomechanical function and reduce low back pain. In this work, a new thermo-sensitive injectable hydrogels with tunable thermo-sensitivity and enhanced stability were developed with N-hexanoylation of glycol chitosan (GC) for treatment of degenerative disc disease, and their physico-chemical and biological properties were evaluated. The sol-gel transition temperature of the hydrogels was controlled in a range of 23-56 °С, depending on the degree of hexanoylation and the polymer concentration...
March 15, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29352906/preparation-of-composite-hydroxybutyl-chitosan-sponge-and-its-role-in-promoting-wound-healing
#18
Shihao Hu, Shichao Bi, Dong Yan, Zhongzheng Zhou, Guohui Sun, Xiaojie Cheng, Xiguang Chen
In this work, a composite sponge was produced by physically mixing hydroxybutyl chitosan with chitosan to form a porous spongy material through vacuum freeze-drying. Hydrophilic and macroporous composite hydroxybutyl chitosan sponge was developed via the incorporation of chitosan into hydroxybutyl chitosan. The composite sponge showed higher porosity (about 85%), greater water absorption (about 25 times), better softness and lower blood-clotting index (BCI) than those of chitosan sponge and hydroxybutyl chitosan sponge...
March 15, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29352755/a-mos1-transposase-in-vivo-assay-to-screen-new-hiv-1-integrase-inhibitors
#19
Mariana Cancian, Elgion L S Loreto
The integrase and transposase enzymes of retrovirus and transposons, respectively, share the catalytic DDE domain. In vitro assays showed that inhibitors of HIV-1 integrase generally inhibit the mariner Mos1 transposase. Using a Drosophila strain in which the mobilisation of the mariner element can be quantified by mosaic eyes, we showed that flies maintained in medium containing 210 µM to 4 mM of raltegravir, or 1 or 2 mM of dolutegravir, which are HIV-1 integrase inhibitor used in AIDS treatment, have 23-33% less somatic mobilisation in mosaic eyes when treated with raltegravir and 28-32% when treated with dolutegravir...
January 19, 2018: Genetica
https://www.readbyqxmd.com/read/29352735/s-nitrosated-poly-propylene-sulfide-nanoparticles-for-enhanced-nitric-oxide-delivery-to-lymphatic-tissues
#20
Alex Schudel, Lauren F Sestito, Susan N Thomas
Nitric oxide (NO) is a therapeutic implicated for the treatment of diseases affecting lymphatic tissues, which range from infectious and cardiovascular diseases to cancer. Existing technologies available for NO therapy, however, provide poor bioactivity within lymphatic tissues. In this work, we address this technology gap with a NO encapsulation and delivery strategy leveraging the formation of S-nitrosothiols on lymphatic-targeting Pluronic-stabilized, poly(propylene sulfide)-core nanoparticles (SNO-NP). We evaluated in vivo the lymphatic versus systemic delivery of NO resulting from intradermal administration of SNO-NP benchmarked against a commonly used, commercially available small molecule S-nitrosothiol NO donor, examined signs of toxicity systemically as well as localized to the site of injection, and investigated SNO effects on lymphatic transport and NP uptake by LN-resident cells...
January 20, 2018: Journal of Biomedical Materials Research. Part A
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