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https://www.readbyqxmd.com/read/29793112/3d-printed-membrane-as-an-alternative-to-amniotic-membrane-for-ocular-surface-conjunctival-defect-reconstruction-an-in-vitro-in-vivo-study
#1
Shima Dehghani, Morteza Rasoulianboroujeni, Hamed Ghasemi, Saeed Heidari Keshel, Zohreh Nozarian, Mohammad Naser Hashemian, Mehran Zarei-Ghanavati, Golshan Latifi, Reza Ghaffari, Zhanfeng Cui, Hua Ye, Lobat Tayebi
BACKGROUND: The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries. METHODS: 3D printing technique was employed to fabricate the membrane based on gradient design. Prior to printing, rheometry was employed to optimize the ink composition...
May 11, 2018: Biomaterials
https://www.readbyqxmd.com/read/29793104/uptake-elimination-and-biotransformation-of-n-ethyl-perfluorooctane-sulfonamide-n-etfosa-by-the-earthworms-eisenia-fetida-after-in-vivo-and-in-vitro-exposure
#2
Shuyan Zhao, Bohui Wang, Lingyan Zhu, Tiankun Liang, Meng Chen, Liping Yang, Jingping Lv, Lifen Liu
N-ethyl perfluorooctane sulfonamide (N-EtFOSA) is commonly known as the active ingredient of sulfluramid. It can be degraded to perfluorooctane sulfonic acid (PFOS) in biota and environment. Earthworms (Eisenia fetida) were exposed with N-EtFOSA to examine the bioaccumulation, elimination and metabolism of N-EtFOSA by the earthworms after in vivo and in vitro exposure. N-EtFOSA could be biodegraded in quartz sands to perfluorooctane sulfonamide (FOSA) and PFOS. In the in vivo tests, in addition to parent N-EtFOSA, three metabolites, including perfluorooctane sulfonamide acetate (FOSAA), FOSA and PFOS also accumulated in earthworms as a result of N-EtFOSA biotransformation, with FOSA as the predominant metabolite...
May 21, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29793091/effects-of-surface-modification-of-as-2-o-3-loaded-plga-nanoparticles-on-its-anti-liver-cancer-ability-an-in-vitro-and-in-vivo-study
#3
Xiaoli Song, Juan You, Hongxia Shao, Caifeng Yan
As2 O3 -loaded nanoparticles (NPs) based on poly (lactic-co-glycolic acid) (PLGA) were prepared by double emulsion-solvent evaporation method. Then Lactose acid (LA) and/or PEG were modified onto the NPs by chemical covalent coupling through -NH2 and -COOH. The FTIR results showed that LA and PEG could be successfully modified onto the surface of the NPs. All the As2 O3 -loaded PLGA NPs (As2 O3 @PLGA NPs) presented suitable physical stability, favorable size, and spherical shape. The in vitro release rate of As2 O3 from the NPs depended on the surface of the NPs...
May 21, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29793089/gold-and-gold-silver-alloy-nanoparticles-enhance-the-myogenic-differentiation-of-myoblasts-through-p38-mapk-signaling-pathway-and-promote-in-vivo-skeletal-muscle-regeneration
#4
Juan Ge, Kai Liu, Wen Niu, Mi Chen, Min Wang, Yumeng Xue, Chuanbo Gao, Peter X Ma, Bo Lei
Under the severe trauma condition, the skeletal muscles regeneration process is inhibited by forming fibrous scar tissues. Understanding the interaction between bioactive nanomaterials and myoblasts perhaps has important effect on the enhanced skeletal muscle tissue regeneration. Herein, we investigate the effect of monodispersed gold and gold-silver nanoparticles (AuNPs and Au-AgNPs) on the proliferation, myogenic differentiation and associated molecular mechanism of myoblasts (C2C12), as well as the in vivo skeletal muscle tissue regeneration...
May 18, 2018: Biomaterials
https://www.readbyqxmd.com/read/29793088/decellularized-and-matured-esophageal-scaffold-for-circumferential-esophagus-replacement-proof-of-concept-in-a-pig-model
#5
Guillaume Luc, Guillaume Charles, Caroline Gronnier, Magali Cabau, Charlotte Kalisky, Mallory Meulle, Reine Bareille, Samantha Roques, Lionel Couraud, Johanna Rannou, Laurence Bordenave, Denis Collet, Marlène Durand
Surgical resection of the esophagus requires sacrificing a long portion of it. Its replacement by the demanding gastric pull-up or colonic interposition techniques may be avoided by using short biologic scaffolds composed of decellularized matrix (DM). The aim of this study was to prepare, characterize, and assess the in vivo remodeling of DM and its clinical impact in a preclinical model. A dynamic chemical and enzymatic decellularization protocol of porcine esophagus was set up and optimized. The resulting DM was mechanically and biologically characterized by DNA quantification, histology, and histomorphometry techniques...
May 17, 2018: Biomaterials
https://www.readbyqxmd.com/read/29793074/cross-linked-electrospun-cartilage-acellular-matrix-poly-caprolactone-co-lactide-co-glycolide-nanofiber-as-an-antiadhesive-barrier
#6
Jin Woo Lee, Joon Yeong Park, Seung Hun Park, Min Ju Kim, Bo Ram Song, Hee-Woong Yun, Tae Woong Kang, Hak Soo Choi, Young Jick Kim, Byoung Hyun Min, Moon Suk Kim
In this work, we chose cartilage acellular matrix (CAM) as a promising antiadhesive material because CAM effectively inhibits the formation of blood vessels, and we used electrospinning to prepare antiadhesive barriers. Additionally, we synthesized N-hydroxysuccinimide (NHS)-poly(caprolactone-co-lactide-co-glycolide)-NHS (MP) copolymers (to tune degradation) as a cross-linking agent for CAM. This is the first report on the development of electrospun cross-linked (Cx) CAM/MP (CA/P) nanofiber (NF) (Cx-CA/P-NF) with a tunable degradation period as an antiadhesive barrier...
May 21, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29793073/bubble-generating-polymersomes-loaded-with-both-indocyanine-green-and-doxorubicin-for-effective-chemotherapy-combined-with-photothermal-therapy
#7
Dunwan Zhu, Fan Fan, Chenlu Huang, Zhiming Zhang, Yu Qin, Li Lu, Hai Wang, Xu Jin, Hanxue Zhao, Hu Yang, Chao Zhang, Jun Yang, Zhipeng Liu, Hongfan Sun, Xigang Leng, Deling Kong, Linhua Zhang
The combination of chemotherapy and photothermaltherapy (PTT) via stimuli-responsive nanovesicles has great potential in tumor treatment. In the present study, bubble-generating polymersomes, which can generate bubbles in response to low pH or hyperthermia, were fabricated to simultaneously encapsulate chemotherapeutic drug and photosensitizing agent for the synergistic chemo-photothermal tumor therapy. Photosensitizer indocyanine green (ICG) was encapsulated into the bilayer of polymersomes formed by amphiphilic triblock copolymer PCL8000 -PEG8000 -PCL8000 through thin film re-hydration method, while chemotherapeutic doxorubicin (DOX) was loaded into the hydrophilic lumen using a transmembrane ammonium bicarbonate gradient loading procedure...
May 21, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29793062/functional-mri-of-brain-physiology-in-aging-and-neurodegenerative-diseases
#8
J Jean Chen
Brain aging and associated neurodegeneration constitute a major societal challenge as well as one for the neuroimaging community. A full understanding of the physiological mechanisms underlying neurodegeneration still eludes medical researchers, fuelling the development of in vivo neuroimaging markers. Hence it is increasingly recognized that our understanding of neurodegenerative processes likely will depend upon the available information provided by imaging techniques. At the same time, the imaging techniques are often developed in response to the desire to observe certain physiological processes...
May 21, 2018: NeuroImage
https://www.readbyqxmd.com/read/29793061/microstructural-imaging-of-the-human-brain-with-a-super-scanner-10-key-advantages-of-ultra-strong-gradients-for-diffusion-mri
#9
D K Jones, D C Alexander, R Bowtell, M Cercignani, F Dell'Acqua, D J McHugh, K L Miller, M Palombo, G J M Parker, U S Rudrapatna, C M W Tax
The key component of a microstructural diffusion MRI 'super-scanner' is a dedicated high-strength gradient system that enables stronger diffusion weightings per unit time compared to conventional gradient designs. This can, in turn, drastically shorten the time needed for diffusion encoding, increase the signal-to-noise ratio, and facilitate measurements at shorter diffusion times. This review, written from the perspective of the National Facility for In Vivo MR Imaging of Human Tissue Microstructure, an initiative to establish a shared 300 mT/m-gradient facility amongst the microstructural imaging community, describes ten advantages of ultra-strong gradients for microstructural imaging...
May 21, 2018: NeuroImage
https://www.readbyqxmd.com/read/29793054/bacillus-clausii-inhibits-bone-loss-by-skewing-treg-th17-cell-equilibrium-in-postmenopausal-osteoporotic-mice-model
#10
Hamid Y Dar, Subhashis Pal, Prashant Shukla, Pradyumna K Mishra, Geetanjali B Tomar, Naibedya Chattopadhyay, Rupesh K Srivastava
OBJECTIVES: Postmenopausal osteoporosis is one of most commonly occurring skeletal diseases leading to bone loss and fragility. Probiotics have been associated with various immunomodulatory properties and thus can be exploited to enhance bone health. In the present study, we report, to our knowledge for the first time, that oral administration of Bacillus clausii (BC) in postmenopausal osteoporotic (OVX) mice model enhances bone health. METHODS: BC was selected as probiotic of choice due to its established immunomodulatory properties...
March 20, 2018: Nutrition
https://www.readbyqxmd.com/read/29792946/investigation-of-nonalcoholic-fatty-liver-disease-induced-drug-metabolism-by-comparative-global-toxicoproteomics
#11
Ann-Yae Na, Jung Jae Jo, Ok Kwang Kwon, Riya Shrestha, Piljoung Cho, Kyu Min Kim, Sung Hwan Ki, Tae Hee Lee, Tae Won Jeon, Tae Cheon Jeong, Sangkyu Lee
Non-alcoholic fatty liver disease (NAFLD) includes conditions such as steatosis, non-alcoholic steatohepatitis, and ultimately hepatocellular carcinoma. Although the pathology of NAFLD is well-established, NAFLD-induced drug metabolism mediated by cytochrome P450 (CYP) in the liver has remained largely unexplored. Therefore, we investigated NAFLD-induced drug metabolism mediated by CYP by quantitative toxicoproteomics analysis. After administration of a methionine-choline deficient (MCD) diet to induce development of NAFLD, tandem mass tags-based liquid chromatography-tandem mass spectrometry analysis was conducted to investigate the dynamics of hepatic proteins...
May 21, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29792920/inner-ear-drug-delivery-recent-advances-challenges-and-perspective
#12
Jinsong Hao, S Kevin Li
Effective and safe treatment of auditory and vestibular diseases has become increasingly dependent on inner ear drug delivery systems. This review highlights recent advances in inner ear drug delivery research and technologies. The focus is on strategies to overcome delivery barriers and to improve drug residence and targeting, with special attention to in vivo animal and human studies. The research in gene and stem cell delivery to the inner ear is briefly reviewed. Newly developed research tools to address experimental challenges and safety issues are discussed...
May 21, 2018: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29792889/ros-triggered-degradable-iron-chelating-nanogels-safely-improving-iron-elimination-in-vivo
#13
Zhi Liu, Jing Qiao, Tamas Nagy, May P Xiong
Iron-mediated generation of highly toxic Reactive Oxygen Species (ROS) plays a major role in the process leading to iron overload-related diseases. The long-term subcutaneous administration of Deferoxamine (DFO) is currently clinically-approved to improve patient symptoms and survival. However, non-specific toxicity and short circulation times of the drug in humans often leads to poor patient compliance. Herein, thioketal-based ROS-responsive polymeric nanogels containing DFO moieties (rNG-DFO) were designed to chelate iron and to degrade under oxidative stimuli into fragments <10 nm to enhance excretion of iron-bound chelates...
May 21, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29792878/deoxyelephantopin-ameliorates-lipopolysaccharides-lps-induced-memory-impairments-in-rats-evidence-for-its-anti-neuroinflammatory-properties
#14
Shathiswaran N Andy, Vijayapandi Pandy, Zazali Alias, Habsah Abdul Kadir
AIM: Neuroinflammation is a critical pathogenic mechanism of most neurodegenerative disorders especially, Alzheimer's disease (AD). Lipopolysaccharides (LPS) are known to induce neuroinflammation which is evident from significant upsurge of pro-inflammatory mediators in in vitro BV-2 microglial cells and in vivo animal models. In present study, we investigated anti-neuroinflammatory properties of deoxyelephantopin (DET) isolated from Elephantopus scaber in LPS-induced neuroinflammatory rat model...
May 21, 2018: Life Sciences
https://www.readbyqxmd.com/read/29792863/identification-of-immunodominant-cd8-epitope-in-the-stalk-domain-of-influenza-b-viral-hemagglutinin
#15
Abenaya Muralidharan, Caroline Gravel, Amparo Duran, Louise Larocque, Changgui Li, Adrian Zetner, Gary Van Domselaar, Lisheng Wang, Xuguang Li
Human infections by type B influenza virus constitute about 25% of all influenza cases. The viral hemagglutinin is comprised of two subunits, HA1 and HA2. While HA1 is constantly evolving in an unpredictable fashion, the HA2 subunit is highly conserved, making it a potential candidate for a universal vaccine. However, immunodominant epitopes in the HA2 subunit remain largely unknown. To delineate MHC Class I epitopes, we first identified 9-mer H-2Kd -restricted CD8 T cell epitopes in the HA2 domain by in silico analyses, followed by evaluating the immunodominance of these peptides in mice challenged with the virus...
May 21, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29792850/microrna-182-inhibits-hcmv-replication-through-activation-of-type-i-ifn-response-by-targeting-foxo3
#16
Xia He, Junfang Teng, Can Cui, Dongrui Li, Lijun Wen
Human cytomegalovirus (HCMV) has led to kinds of clinical disorders and great morbidity worldwide, such as sensorineural hearing loss (SNHL), mental retardation, and developmental delays in immunocompromised individuals. Previous studies reported that HCMV has evolved a variety of mechanisms to evade the immune system, such as dysregulation of miRNAs. However, reports concerning the role of miRNA in HCMV infection are limited. Here, we reported that a host microRNA, miR-182, was significantly up-regulated by HCMV infection in U-251MG and NPCs cells...
May 21, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29792833/self-diffusion-of-water-soluble-fullerene-derivatives-in-mouse-erythrocytes
#17
Irina Avilova, Ekaterina Khakina, Ol''ga Kraevaya, Alexander Kotelnikov, Raisa Kotelnikova, Pavel Troshin, Vitaliy Volkov
Self-diffusion of water-soluble fullerene derivative (WSFD) C60 [S(CH2 )3 SO3 Na]5 H in mouse red blood cells (RBC) was characterized by 1 H pulsed field gradient NMR technique. It was found that a fraction of fullerene molecules (~13% of the fullerene derivative added in aqueous RBC suspension) shows a self-diffusion coefficient of (5.5 ± 0.8)·10-12  m2 /s, which is matching the coefficient of the lateral diffusion of lipids in the erythrocyte membrane (DL  = (5.4 ± 0.8)·10-12  m2 /s). This experimental finding evidences the absorption of the fullerene derivative by RBC...
May 21, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29792808/eradication-of-established-tumors-by-chemically-self-assembled-nanoring-csan-targeted-t-cells
#18
Jacob Petersburg, Jingjing Shen, Clifford M Csizmar, Katherine A Murphy, Justin Spanier, Kari Gabrielse, Thomas S Griffith, Brian Fife, Carston R Wagner
Our laboratory has developed chemically self-assembled nanorings (CSANs) as prosthetic antigen receptors (PARs) for the non-genetic modification of T-cell surfaces. PARs have been successfully employed in vitro to activate T cells for the selective killing of leukemia cells. However, PAR efficacy has yet to be evaluated in vivo or against solid tumors. Therefore, we developed bispecific PARs that selectively target the human CD3 receptor and human Epithelial Cell Adhesion Molecule (EpCAM), which is overexpressed on multiple carcinomas and cancer stem cells...
May 24, 2018: ACS Nano
https://www.readbyqxmd.com/read/29792806/fate-of-fluorescence-labels-their-adsorption-and-desorption-kinetics-to-silver-nanoparticles
#19
Claudia Kästner, Linda Böhmert, Albert Braeuning, Alfonso Lampen, Andreas F Thünemann
Silver nanoparticles are among the most widely used und produced nanoparticles. Due to their frequent application in consumer products, the assessment of their toxicological potential has seen a renewed importance. A major difficulty is the traceability of nanoparticles in in vitro and in vivo experiments. Even if the particles are labeled, for example by a fluorescent marker, the dynamic exchange of ligands often prohibits their spatial localization. Our study provides an insight into the adsorption and desorption kinetics of two different fluorescent labels on silver nanoparticles with a core radius of 3 nm by dynamic light scattering (DLS), small-angle X-ray scattering (SAXS) and fluorescence spectroscopy...
May 24, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29792799/in-vivo-evaluation-of-reduction-responsive-alendronate-hyaluronan-curcumin-polymer-drug-conjugates-for-targeted-therapy-of-bone-metastatic-breast-cancer
#20
Kaili Wang, Chunjing Guo, Xue Dong, Yueming Yu, Bingjie Wang, Wanhui Liu, Daquan Chen
Many cancers such as human breast cancer and lung cancer easily metastasize to bones, leading to the formation of secondary tumors in advanced stage. Based on the CD44-targeted effect of oHA and bone-targeted effect of ALN, we prepared a reduction-responsive, CD44 receptor-targeting and bone-targeting nano-micelle, called CUR-loaded ALN-oHA-S-S-CUR micelles. In this study, we aimed to evaluate the antitumor activity and bone-targeting ability of CUR-loaded ALN-oHA-S-S-CUR micelles. The in vivo experiment results showed that a larger number of micelles was gathered in the bone metastatic tumor tissue and reduced the bone destruction...
May 24, 2018: Molecular Pharmaceutics
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