keyword
MENU ▼
Read by QxMD icon Read
search

dihydroethidium

keyword
https://www.readbyqxmd.com/read/28319651/mediterranean-tomato-based-sofrito-protects-against-vascular-alterations-in-obese-zucker-rats-by-preserving-no-bioavailability
#1
Rosalia Rodriguez-Rodriguez, Francesc Jiménez-Altayó, Laia Alsina, Yara Onetti, Jose F de Alvarenga, Carmen Claro, Elena Ogalla, Nuria Casals, Rosa M Lamuela-Raventos
SCOPE: Sofrito, a key component of the Mediterranean diet, provides nutritional interest due to its high content in bioactive compounds from tomato and olive oil, and especially to the lipid matrix in which these compounds are found. In this study, the potential beneficial effects of dietary intake of sofrito on obesity-related vascular alterations were explored in obese Zucker rats. METHODS AND RESULTS: Obese and lean rats were fed a control diet supplemented or not with 2% (w/w) sofrito for 8 weeks...
March 20, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28316022/suppression-of-inner-mitochondrial-membrane-peptidase-2-like-immp2l-gene-exacerbates-hypoxia-induced-neural-death-under-high-glucose-condition
#2
Yi Ma, Zijing Zhang, Zhirong Chen, Nina Ma, Shihui Sun, Jingwen Zhang, Xinli Ni, Jianzhong Zhang, P Andy Li
It is known that diabetes hyperglycemia enhances cerebral ischemia and reperfusion induced damage. We have previously shown that mutation of inner mitochondrial membrane peptidase 2-like (IMMP2L) increases brain damage caused by transient cerebral ischemia. In this study, we attempt to examine the impact of IMMP2L deficiency on an in vitro model that mimics the diabetic hypoxic conditions. Normal IMMP2L wild type and IMMP2L gene deleted HT22 cells were cultured. Hypoxia was induced under high glucose and acidic conditions with 4 h of oxygen deprivation...
March 18, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28260010/astragaloside-iv-inhibits-isoprenaline%C3%A2-induced-cardiac-fibrosis-by-targeting-the-reactive-oxygen-species-mitogen%C3%A2-activated-protein-kinase-signaling-axis
#3
Hongliang Dai, Guizhi Jia, Meili Lu, Chunguang Liang, Yue Wang, Hongxin Wang
Cardiac fibrosis is considered an important pathological mechanism in the progression of cardiac remodeling and heart failure. Astragaloside IV (AsIV) is a major active ingredient in Astragalus membranaceus. In a preliminary experiment, it was demonstrated that this naturally occurring substance exhibited cardioprotective effects via preventing cardiomyocyte hypertrophy and apoptosis. The present study aimed to investigate the effects of AsIV on β‑adrenergic receptor (β‑AR)‑mediated cardiac fibrosis, and the associated mechanism...
February 17, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28259164/n-butanol-extracts-of-morinda-citrifolia-suppress-advanced-glycation-end-products-age-induced-inflammatory-reactions-in-endothelial-cells-through-its-anti-oxidative-properties
#4
Yuji Ishibashi, Takanori Matsui, Fumiyuki Isami, Yumi Abe, Tatsuya Sakaguchi, Yuichiro Higashimoto, Sho-Ichi Yamagishi
BACKGROUND: Advanced glycation end products (AGEs), senescent macroprotein derivatives formed during a normal aging process and acceleratedly under diabetic conditions, play a role in atherosclerotic cardiovascular disease. AGEs cause endothelial cell (EC) damage, an initial trigger for atherosclerosis through the interaction with a receptor for AGEs (RAGE). We have previously shown that n-butanol extracts of Morinda citrifolia (noni), a plant belonging to the family Rubiaceae, block the binding of AGEs to RAGE in vitro...
March 4, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28246278/renal-denervation-in-the-acute-phase-of-ischemic-stroke-provides-brain-protection-in-hypertensive-rats
#5
Yu Hasegawa, Takashi Nakagawa, Kunihiko Matsui, Shokei Kim-Mitsuyama
BACKGROUND AND PURPOSE: Renal denervation (RD) may protect against cardiovascular diseases regardless of blood pressure (BP)-lowering effect. We hypothesized that RD can improve the outcome of acute ischemic stroke (AIS) in hypertensive rats. METHODS: An AIS model of spontaneously hypertensive stroke-prone rats (SHRSPs) was prepared by 90-minute middle cerebral artery occlusion followed by reperfusion. At 30 minutes poststroke, the SHRSPs were subjected to (1) sham operation or (2) RD to determine the beneficial effects of RD posttreatment...
February 28, 2017: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/28245300/anti-inflammatory-and-antioxidative-effects-of-camellia-japonica-on-human-corneal-epithelial-cells-and-experimental-dry-eye-in-vivo-and-in-vitro-study
#6
Hyo Seok Lee, Joo-Hee Choi, Lian Cui, Ying Li, Jee Myung Yang, Je-Jung Yun, Ji Eun Jung, Won Choi, Kyung Chul Yoon
Purpose: To analyze the anti-inflammatory and antioxidative effects of Camellia japonica (CJ) on human corneal epithelial (HCE) cells and its therapeutic effects in a mouse model of experimental dry eye (EDE). Methods: Camellia japonica extracts of varying concentrations (0.001%, 0.01%, and 0.1%) were used to treat HCE cells. Dichlorofluorescein diacetate (DCF-DA) and dihydroethidium (DHE) assays were performed. The production of peroxiredoxin (PRX) 1-6 and manganese-dependent superoxide dismutase (MnSOD) in HCE cells was assessed using Western blot analysis...
February 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28238401/flavin-containing-enzymes-as-a-source-of-reactive-oxygen-species-in-hema-induced-apoptosis
#7
Helmut Schweikl, Marialucia Gallorini, Markus Forstner, Christine Petzel, Carola Bolay, Karl-Anton Hiller, Amelia Cataldi, Stephanie Krifka, Wolfgang Buchalla
OBJECTIVE: Oxidative stress induced by compounds of dental composites like 2-hydroxyethyl methacrylate (HEMA) due to excess formation of reactive oxygen species (ROS) disturbs vital cell functions leading to apoptosis. The sources of ROS in cells exposed to resin monomers are unknown. The present study investigates functions of flavin-containing ROS and RNS (reactive nitrogen species) producing enzymes in cells exposed to HEMA. METHODS: The formation of oxidative stress in RAW264...
February 18, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28203527/superoxide-increases-angiotensin-ii-at1-receptor-function-in-human-kidney-2-cells
#8
Mohammad Saleem, Indira Pokkunuri, Mohammad Asghar
The redox-sensitive Sp family transcription factor has been linked to the regulation of angiotensin II type 1 receptor (AT1R). However, the exact mechanism of AT1R regulation in renal cells is poorly understood. We tested the specificity of reactive oxygen species (ROS), superoxide vs. hydrogen peroxide (H2O2), and the specific role of Sp3 transcription factor, if any, in the regulation of AT1R in human kidney cells (HK2 cells). Superoxide dismutase (SOD) inhibitor diethyldithiocarbamate (DETC), but not H2O2 treatment, increased fluorescence levels of superoxide probe dihydroethidium (DHE)...
December 2016: FEBS Open Bio
https://www.readbyqxmd.com/read/28192190/%C3%AE-7-integrin-and-madcam-1-play-opposing-roles-during-the-development-of-non-alcoholic-steatohepatitis
#9
Hannah K Drescher, Angela Schippers, Thomas Clahsen, Hacer Sahin, Heidi Noels, Mathias Hornef, Norbert Wagner, Christian Trautwein, Konrad L Streetz, Daniela C Kroy
BACKGROUND & AIMS: Non-alcoholic steatohepatitis (NASH) is a leading cause of chronic liver disease in Western countries. It is unclear how infiltrating leukocytes affect NASH-development. Our study aims to investigate the role of the homing/receptor, pair mucosal addressin cell adhesion molecule-1 (MAdCAM-1)/β7-Integrin, on immune cell recruitment and disease progression in a steatohepatitis model. METHODS: Constitutive β7-Integrin deficient (β7(-/-)) and MAdCAM-1 deficient (MAdCAM-1(-/-)) mice were fed a high fat diet (HFD) for 26weeks or methionine-choline-deficient-diet (MCD) for 4weeks...
February 10, 2017: Journal of Hepatology
https://www.readbyqxmd.com/read/28116721/measurement-of-superoxide-production-and-nadph-oxidase-activity-by-hplc-analysis-of-dihydroethidium-oxidation
#10
Denise C Fernandes, Renata C Gonçalves, Francisco R M Laurindo
The fluorogenic probe dihydroethidium (DHE) is widely used for detecting intracellular superoxide. DHE oxidation by superoxide generates specifically the compound 2-hydroxyethidium (2-E(+)OH), so that 2-E(+)OH detection confers specificity to superoxide assessment among many other reactive oxygen species. However, DHE oxidation in biological systems leads to formation of other fluorescent products, particularly ethidium, usually formed at higher quantities than 2-E(+)OH. Since both 2-E(+)OH and ethidium are fluorescent, their identification and quantification is possible only after their physical separation by HPLC...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28099583/swimming-training-prevents-coronary-endothelial-dysfunction-in-ovariectomized-spontaneously-hypertensive-rats
#11
E R G Claudio, S A Almeida, V Mengal, G A Brasil, C H Santuzzi, R V Tiradentes, S A Gouvea, N S Bissoli, R L Santos, G R Abreu
Estrogen deficiency and hypertension are considered major risk factors for the development of coronary heart disease. On the other hand, exercise training is considered an effective form to prevent and treat cardiovascular diseases. However, the effects of swimming training (SW) on coronary vascular reactivity in female ovariectomized hypertensive rats are not known. We aimed to evaluate the effects of SW on endothelium-dependent coronary vasodilation in ovariectomized hypertensive rats. Three-month old spontaneously hypertensive rats (SHR, n=50) were divided into four groups: sham (SH), sham plus swimming training (SSW), ovariectomized (OVX), and ovariectomized plus swimming training (OSW)...
January 16, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28039404/free-radical-scavenging-decreases-endothelin-1-excretion-and-glomerular-albumin-permeability-during-type-1-diabetes
#12
Mohamed A Saleh, Carmen De Miguel, David I Stevens, Pamela K Carmines, David M Pollock, Jennifer S Pollock
Increased renal endothelin-1 (ET-1) production and an ETA receptor-dependent increase in glomerular albumin permeability (Palb) accompany type 1 diabetes mellitus (T1D). We hypothesized that T1D-induced oxidative stress contributes to renal ET-1 production and glomerular Palb Male rats with streptozotocin-induced T1D were provided free access to drinking water without additives (T1D rats) or containing the free radical scavenger tempol (1 mmol/L; T1D+Tempol). After 3 weeks, T1D+Tempol rats displayed lower urinary excretion of thiobarbituric acid reactive substances and glomerular superoxide production (dihydroethidium staining) compared to T1D rats...
December 2016: Physiological Reports
https://www.readbyqxmd.com/read/28005918/the-plant-hormone-cytokinin-confers-protection-against-oxidative-stress-in-mammalian-cells
#13
Eman M Othman, Muhammed Naseem, Eman Awad, Thomas Dandekar, Helga Stopper
Modulating key dynamics of plant growth and development, the effects of the plant hormone cytokinin on animal cells gained much attention recently. Most previous studies on cytokinin effects on mammalian cells have been conducted with elevated cytokinin concentration (in the μM range). However, to examine physiologically relevant dose effects of cytokinins on animal cells, we systematically analyzed the impact of kinetin in cultured cells at low and high concentrations (1nM-10μM) and examined cytotoxic and genotoxic conditions...
2016: PloS One
https://www.readbyqxmd.com/read/27995330/piceatannol-attenuates-homocysteine-induced-endoplasmic-reticulum-stress-and-endothelial-cell-damage-via-heme-oxygenase-1-expression
#14
Jin-Sang Kil, Sun-Oh Jeong, Hun-Taeg Chung, Hyun-Ock Pae
A growing body of evidence implicates endoplasmic reticulum (ER)-induced cellular dysfunction and apoptosis as important factors to a variety of diseases. In endothelial cells (ECs), the sulfur-containing amino acid homocysteine (Hcy) causes EC apoptosis and reactive oxygen species (ROS) generation through induction of ER stress. Here, we have investigated whether piceatannol (Pic), a resveratrol analog, could protect ECs against Hcy-induced apoptosis, oxidative stress and ER stress, with specific emphasis on heme oxygenase-1 (HO-1)...
April 2017: Amino Acids
https://www.readbyqxmd.com/read/27992310/a-kinetic-study-of-reactive-oxygen-species-in-rainbow-trout-hepatocytes-by-fluorometry
#15
Mazyar Yazdani, Ketil Hylland
The kinetics of reactive oxygen species (ROS) formation in a primary culture of rainbow trout hepatocytes was investigated using three fluorescent probes: 5-,6-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate (CM-H2DCFDA), dihydrorhodamine 123 (DHR 123), and dihydroethidium (DHE). The cell cultures were loaded with the three probes, separately. Hepatocytes were then exposed to Cu (0.15-10 mM) in serum-free Leibovitz's medium for 30 min before being quantified by a fluorescence plate reader during 30 min...
2016: Journal of Environmental Pathology, Toxicology and Oncology
https://www.readbyqxmd.com/read/27989546/superoxide-anion-generation-and-oxidative-stress-in-methylmercury-induced-endothelial-toxicity-in-vitro
#16
Heloisa Ghizoni, Viviane de Souza, Marcos Raniel Straliotto, Andreza Fabro de Bem, Marcelo Farina, Mariana Appel Hort
Emerging evidence has pointed to mercury exposure as a risk factor for hypertension, atherosclerosis, myocardial infarction and coronary heart disease. However, the underlying mechanisms are not well understood. This study investigated potential toxic effects of low concentrations of methylmercury (MeHg) in cultured bovine aortic endothelial cells (BAECs) and the possible involvement of reactive species, particularly superoxide anion, in mediating such toxicity. MeHg treatment increased the oxidation of 2',7'-dichlorodihydrofluorescein diacetate (a general probe for reactive species) and dihydroethidium, a specific probe for superoxide anion...
October 27, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27930804/increased-mitochondrial-ros-generation-mediates-the-loss-of-pvat-anticontractile-effect-in-hfd-treated-obese-mice
#17
Rafael Menezes da Costa, Rafael S Fais, Carlos R P Dechandt, Paulo Louzada-Junior, Luciane C Alberici, Núbia S Lobato, Rita C Tostes
Obesity is associated with structural and functional changes in the perivascular adipose tissue (PVAT), favoring the release of reactive oxygen species (ROS), vasoconstrictor and proinflammatory factors. Tumor necrosis factor alpha (TNF-α) induces vascular dysfunction and is produced by the PVAT. We tested the hypothesis that obesity-associated PVAT dysfunction is mediated by augmented mitochondrial ROS (mROS) generation due to increased TNF-α production in this tissue. Aortae from high fat diet (HFD)-fed obese mice displayed increased contractions to phenylephrine (PE) and loss of PVAT anticontractile effect...
December 8, 2016: British Journal of Pharmacology
https://www.readbyqxmd.com/read/27930351/hydrogen-peroxide-responsive-nanoparticle-reduces-myocardial-ischemia-reperfusion-injury
#18
Soochan Bae, Minhyung Park, Changsun Kang, Serkan Dilmen, Tae Hi Kang, Dong Goo Kang, Qingen Ke, Seung Uk Lee, Dongwon Lee, Peter M Kang
BACKGROUND: During myocardial ischemia/reperfusion (I/R), a large amount of reactive oxygen species (ROS) is produced. In particular, overproduction of hydrogen peroxide (H2O2) is considered to be a main cause of I/R-mediated tissue damage. We generated novel H2O2-responsive antioxidant polymer nanoparticles (PVAX and HPOX) that are able to target the site of ROS overproduction and attenuate the oxidative stress-associated diseases. In this study, nanoparticles were examined for their therapeutic effect on myocardial I/R injury...
November 14, 2016: Journal of the American Heart Association
https://www.readbyqxmd.com/read/27902770/mitochondrial-targeted-decyl-triphenylphosphonium-enhances-2-deoxy-d-glucose-mediated-oxidative-stress-and-clonogenic-killing-of-multiple-myeloma-cells
#19
Jeanine Schibler, Ann M Tomanek-Chalkley, Jessica L Reedy, Fenghuang Zhan, Douglas R Spitz, Michael K Schultz, Apollina Goel
Therapeutic advances have markedly prolonged overall survival in multiple myeloma (MM) but the disease currently remains incurable. In a panel of MM cell lines (MM.1S, OPM-2, H929, and U266), using CD138 immunophenotyping, side population staining, and stem cell-related gene expression, we demonstrate the presence of stem-like tumor cells. Hypoxic culture conditions further increased CD138low stem-like cells with upregulated expression of OCT4 and NANOG. Compared to MM cells, these stem-like cells maintained lower steady-state pro-oxidant levels with increased uptake of the fluorescent deoxyglucose analog...
2016: PloS One
https://www.readbyqxmd.com/read/27893783/role-of-pten-in-oxidative-stress-and-dna-damage-in-the-liver-of-whole-body-pten-haplodeficient-mice
#20
Ezgi Eyluel Bankoglu, Oliver Tschopp, Johannes Schmitt, Philipp Burkard, Daniel Jahn, Andreas Geier, Helga Stopper
Type 2 diabetes (T2DM) and obesity are frequently associated with non-alcoholic fatty liver disease (NAFLD) and with an elevated cancer incidence. The molecular mechanisms of carcinogenesis in this context are only partially understood. High blood insulin levels are typical in early T2DM and excessive insulin can cause elevated reactive oxygen species (ROS) production and genomic instability. ROS are important for various cellular functions in signaling and host defense. However, elevated ROS formation is thought to be involved in cancer induction...
2016: PloS One
keyword
keyword
100150
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"