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https://www.readbyqxmd.com/read/29143242/comparing-reactive-oxygen-species-and-dna-fragmentation-in-semen-samples-of-unexplained-infertile-and-healthy-fertile-men
#1
Zahra Zandieh, Akram Vatannejad, Mahmood Doosti, Sara Zabihzadeh, Mahnaz Haddadi, Leila Bajelan, Batool Rashidi, Saeid Amanpour
BACKGROUND: Male factor infertility has increased to more than 40% during the last decade. About 30% of these couples are diagnosed with unexplained infertility. In fact, reactive oxygen species (ROS), especially superoxide anion (O2(-)·) and hydrogen peroxide (H2O2), play a crucial role in regulation of physiological and pathological processes in spermatozoa. Moreover, since the diagnosis of unexplained infertility just through semen analysis is a matter of much controversy; we aimed to evaluate the levels of ROS and sperm DNA fragmentation in the semen samples of unexplained infertile and fertile control couples...
November 15, 2017: Irish Journal of Medical Science
https://www.readbyqxmd.com/read/29113274/simvastatin-potentiates-doxorubicin-activity-against-mcf-7-breast-cancer-cells
#2
Benjaporn Buranrat, Wanwisa Suwannaloet, Jarinyaporn Naowaboot
Simvastatin is a low density lipoprotein-lowering drug that is widely used to prevent and treat cardiovascular disease by inhibiting the mevalonate pathway. Simvastatin also exhibits inhibitory effects on a number of types of cancer. In the present study, the effects of simvastatin on the activity of doxorubicin in the breast cancer MCF-7 cell line, and the mechanisms by which this interaction occurs were investigated. The effect of simvastatin and doxorubicin treatment, alone and in combination, on the growth of MCF-7 cells was evaluated by a sulforhodamine B and colony formation assay...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29104724/protective-effects-of-oral-astaxanthin-nanopowder-against-ultraviolet-induced-photokeratitis-in-mice
#3
Fumiya Harada, Tetsuro Morikawa, Anton Lennikov, Anthony Mukwaya, Mira Schaupper, Osamu Uehara, Rie Takai, Koki Yoshida, Jun Sato, Yukihiro Horie, Hiroyuki Sakaguchi, Ching-Zong Wu, Yoshihiro Abiko, Neil Lagali, Nobuyoshi Kitaichi
Purpose: Astaxanthin (AST) has a strong antioxidant cellular membrane chaperone protective effect. Recently, a water-soluble nanosized AST (nano-AST) form was produced, which is expected to improve the efficacy of oral intake effects. The purpose of this study was to examine whether oral nano-AST has therapeutic effects on UV-induced photokeratitis in mice. Methods: C57BL/6 mice were administered twice with either nano-AST, AST oil, lutein, or bilberry extracts 3 hours before and shortly before UV irradiation (dose: 400 mJ/cm(2))...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29102637/assessment-of-reactive-oxygen-species-generated-by-electronic-cigarettes-using-acellular-and-cellular-approaches
#4
Jiayuan Zhao, Yipei Zhang, Jennifer D Sisler, Justine Shaffer, Stephen S Leonard, Anna M Morris, Yong Qian, Dhimiter Bello, Philip Demokritou
Electronic cigarettes (e-cigs) have fast increased in popularity but the physico-chemical properties and toxicity of the generated emission remain unclear. Reactive oxygen species (ROS) are likely present in e-cig emission and can play an important role in e-cig toxicity. However, e-cig ROS generation is poorly documented. Here, we generated e-cig exposures using a recently developed versatile exposure platform and performed systematic ROS characterization on e-cig emissions using complementary acellular and cellular techniques: 1) a novel acellular Trolox-based mass spectrometry method for total ROS and hydrogen peroxide (H2O2) detection, 2) electron spin resonance (ESR) for hydroxyl radical detection in an acellular and cellular systems and 3) in vitro ROS detection in small airway epithelial cells (SAEC) using the dihydroethidium (DHE) assay...
October 29, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29036612/cd40l-controls-obesity-associated-vascular-inflammation-oxidative-stress-and-endothelial-dysfunction-in-mice-translational-aspects-for-man
#5
Sebastian Steven, Mobin Dib, Michael Hausding, Fatemeh Kashani, Matthias Oelze, Swenja Kröller-Schön, Alina Hanf, Steffen Daub, Siyer Roohani, Yves Gramlich, Esther Lutgens, Eberhard Schulz, Christian Becker, Karl J Lackner, Hartmut Kleinert, Christoph Knosalla, Beate Niesler, Philipp S Wild, Thomas Münzel, Andreas Daiber
Aims: CD40 ligand (CD40L) signaling controls vascular oxidative stress and related dysfunction in angiotensin-II-induced arterial hypertension by regulating vascular immune cell recruitment and platelet activation. Here we investigated the role of CD40L in experimental hyperlipidemia. Methods and Results: Male wild type and CD40L-/- mice (C57BL/6 background) were subjected to high fat diet for sixteen weeks. Weight, cholesterol, HDL and LDL levels, endothelial function (isometric tension recording), oxidative stress (NADPH oxidase expression, dihydroethidium fluorescence) and inflammatory parameters (inducible nitric oxide synthase, interleukin-6 expression) were assessed...
September 26, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28931831/hyperhomocysteinemia-alters-retinal-endothelial-cells-barrier-function-and-angiogenic-potential-via-activation-of-oxidative-stress
#6
Riyaz Mohamed, Isha Sharma, Ahmed S Ibrahim, Heba Saleh, Nehal M Elsherbiny, Sadanand Fulzele, Khaled Elmasry, Sylvia B Smith, Mohamed Al-Shabrawey, Amany Tawfik
Hyperhomocysteinemia (HHcy) is associated with several human visual disorders, such as diabetic retinopathy (DR) and age-related macular degeneration (AMD). Breakdown of the blood-retinal barrier (BRB) is linked to vision loss in DR and AMD. Our previous work revealed that HHcy altered BRB in retinal endothelial cells in vivo. Here we hypothesize that homocysteine (Hcy) alters retinal endothelial cell barrier function and angiogenic potential via activation of oxidative stress. Human retinal endothelial cells (HRECs) treated with and without different concentrations of Hcy showed a reduction of tight junction protein expression, increased FITC dextran leakage, decreased transcellular electrical resistance and increased angiogenic potential...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28925291/isolation-of-a-novel-compound-mimo2-from-the-methanolic-extract-of-moringa-oleifera-leaves-protective-effects-against-vanadium-induced-cytotoxity
#7
Olumayowa O Igado, Jan Glaser, Mario Ramos-Tirado, Ezgi Eylül Bankoğlu, Foluso A Atiba, Ulrike Holzgrabe, Helga Stopper, James O Olopade
Moringa oleifera is reported to be a miracle plant, with positive effects on practically every system in the animal body. The methanolic extract of Moringa oleifera leaves was fractionated using liquid-liquid fractionation, column chromatography and preparative high-performance liquid chromatography (HPLC). Bioassay guided fractionation using Ferric Reducing Antioxidant Power (FRAP) was used to determine the fraction with the highest antioxidative power. Chemical structure was elucidated with nuclear magnetic resonance (NMR) spectroscopy...
September 19, 2017: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/28878301/epa-dha-6-1-prevents-angiotensin-ii-induced-hypertension-and-endothelial-dysfunction-in-rats-role-of-nadph-oxidase-and-cox-derived-oxidative-stress
#8
Zahid Rasul Niazi, Grazielle C Silva, Thais Porto Ribeiro, Antonio J León-González, Mohamad Kassem, Abdur Mirajkar, Azhar Alvi, Malak Abbas, Faraj Zgheel, Valérie B Schini-Kerth, Cyril Auger
Eicosapentaenoic acid:docosahexaenoic acid (EPA:DHA) 6:1, an omega-3 polyunsaturated fatty acid formulation, has been shown to induce a sustained formation of endothelial nitric oxide (NO) synthase-derived NO, a major vasoprotective factor. This study examined whether chronic intake of EPA:DHA 6:1 prevents hypertension and endothelial dysfunction induced by angiotensin II (Ang II) in rats. Male Wister rats received orally corn oil or EPA:DHA 6:1 (500 mg kg(-1) per day) before chronic infusion of Ang II (0...
September 7, 2017: Hypertension Research: Official Journal of the Japanese Society of Hypertension
https://www.readbyqxmd.com/read/28840566/photo-induced-oxidative-stress-impairs-mitochondrial-metabolism-in-neurons-and-astrocytes
#9
Elena Berezhnaya, Maria Neginskaya, Anatoly B Uzdensky, Andrey Y Abramov
Photodynamic therapy is selective destruction of cells stained with a photosensitizer upon irradiation with light at a specific wavelength in the presence of oxygen. Cell death upon photodynamic treatment is known to occur mainly due to free radical production and subsequent development of oxidative stress. During photodynamic therapy of brain tumors, healthy cells are also damaged; considering this, it is important to investigate the effect of the treatment on normal neurons and glia. We employed live-cell imaging technique to investigate the cellular mechanism of photodynamic action of radachlorin (200 nM) on neurons and astrocytes in primary rat cell culture...
August 24, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28836500/in-vitro-non-thermal-oxidative-stress-response-after-1800-mhz-radiofrequency-radiation
#10
Ana Marija Marjanovic Cermak, Ivan Pavicic, Blanka Tariba Lovakovic, Alica Pizent, Ivancica Trosic
In this study possible connection between radiofrequency exposure (RF) and development of oxidative stress was investigated by measuring impairment in cellular oxidation-reduction balance immediately after RF exposure. Fibroblast cells V79 were exposed for 10, 30 and 60 minutes to 1800 MHz RF radiation. Electric field strength was 30 V/m and specific absorption rate (SAR) was calculated to be 1.6 W/kg. Electromagnetic field was generated within Gigahertz Transversal Electromagnetic Mode cell (GTEM) equipped by signal generator, amplifier and modulator...
October 2017: General Physiology and Biophysics
https://www.readbyqxmd.com/read/28836394/reduced-nrf2-activation-in-pi3k-phosphorylation-impaired-vitiliginous-keratinocytes-increases-susceptibility-to-ros-generating-chemical-induced-apoptosis
#11
Hyangmi Kim, Chang Seo Park, Ai-Young Lee
Keratinocytes in affected epidermis of vitiligo patients are known to have impaired activation of the PI3K/AKT pathway. Based on critical roles of keratinocytes and oxidative stress in vitiligo development, this study examined whether keratinocytes with impaired PI3K activation were more vulnerable to apoptosis caused by oxidative stress from phenolic compounds, p-tert-butylphenol (4-TBP) and hydroquinone (HQ). Cell viability assay, FACS analysis, ELISA for TNF-α or IL-1a, ROS assay, Western blot analysis for Nrf2 expression, and confocal microscopy with anti-Nrf2 and phospho-PI3K antibodies were done on primary cultured normal human keratinocytes with or without PI3K knockdown in the presence or absence of chemical treatment or antioxidant...
August 24, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/28830679/angeli-s-salt-a-nitroxyl-anion-donor-reverses-endothelin-1-mediated-vascular-dysfunction-in-murine-aorta
#12
Brandi M Wynne, Hicham Labazi, Zidonia N Carneiro, Rita C Tostes, R Clinton Webb
Nitroglycerin (Gtn) is a treatment for cardiovascular patients due to its vasodilatory actions, but induces tolerance when given chronically. A proposed mechanism is the superoxide (O2(-))-oxidative stress hypothesis, which suggests that Gtn increases O2(-) production. Nitric oxide (NO) exists in three different redox states; the protonated, reduced state, nitroxyl anion (HNO) is an emerging candidate in vascular regulation. HNO is resistant to scavenging and of particular interest in conditions where high levels of reactive oxygen species (ROS) exist...
November 5, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28822963/%C3%AE-1a-subtype-adrenergic-agonist-therapy-for-failing-right-ventricle
#13
Patrick M Cowley, Guan-Ying Wang, Sunil K Joshi, Philip M Swigart, David H Lovett, Paul C Simpson, Anthony J Baker
Failure of the right ventricle (RV) is a serious disease with a poor prognosis and limited treatment options. Signaling by α1-adrenergic receptors (α1-ARs), in particular the α1A-subtype, mediate cardioprotective effects in multiple heart failure models. Recent studies show that chronic treatment with the α1A-subtype agonist A61603 improves function and survival in a model of LV failure. The goal of this study was to determine if chronic A61603 treatment is beneficial in a RV failure model. We used tracheal instillation of the fibrogenic antibiotic bleomycin in mice to induce pulmonary fibrosis, pulmonary hypertension, and RV failure within 2 wk...
August 19, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28822761/down-regulation-of-vascular-ppar-%C3%AE-contributes-to-endothelial-dysfunction-in-high-fat-diet-induced-obese-mice-exposed-to-chronic-intermittent-hypoxia
#14
Yanan Zhang, Chunlian Zhang, Haiou Li, Jingdong Hou
Obstructive sleep apnea (OSA), characterized by chronic intermittent hypoxia (CIH), is associated with endothelial dysfunction. The prevalence of OSA is linked to an epidemic of obesity. CIH has recently been reported to cause endothelial dysfunction in diet-induced obese animals by exaggerating oxidative stress and inflammation, but the underlying mechanism remains unclear. PPAR-γ, a ligand-inducible transcription factor that exerts anti-oxidant and anti-inflammatory effects, is down-regulated in the peripheral tissues in diet-induce obesity...
October 14, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28805491/intermedin-inhibits-unilateral-ureteral-obstruction-induced-oxidative-stress-via-nadph-oxidase-nox4-and-camp-dependent-mechanisms
#15
Xi Qiao, Lihua Wang, Yanhong Wang, Xiaole Su, Yue Qi, Yun Fan, Zhiqiang Peng
NADPH oxidase Nox4-derived reactive oxygen species (ROS) play important roles in renal fibrosis. Our previous study demonstrated that intermedin (IMD) alleviated unilateral ureteral obstruction (UUO)-induced renal fibrosis by inhibition of ROS. However, the precise mechanisms remain unclear. Herein, we investigated the effect of IMD on Nox4 expression and NADPH oxidase activity in rat UUO model, and explored if these effect were achieved through cAMP-PKA pathway, the important post-receptor signal transduction pathway of IMD, in TGF-β1-stimulated rat proximal tubular cell (NRK-52E)...
November 2017: Renal Failure
https://www.readbyqxmd.com/read/28793197/glycosylation-with-o-linked-%C3%AE-n-acetylglucosamine-o-glcnac-induces-vascular-dysfunction-via-production-of-superoxide-anion-reactive-oxygen-species
#16
Leonardo Souza-Silva, Rhéure Alves-Lopes, Jéssica Silva Miguez, Vanessa Dela Justina, Karla Bianca Neves, Fabíola Leslie Mestriner, Rita C Tostes, Fernanda Regina Giachini, Victor V Lima
Overproduction of superoxide anion (•O2-) and O-linked β-N-acetylglucosamine (O-GlcNAc)-modification in the vascular system are contributors to endothelial dysfunction. This study tested the hypothesis that increased levels of O-GlcNAc-modified proteins contribute to •O2- production via activation of NADPH oxidase, resulting in impaired vasodilation. Rat aortic segments and vascular smooth muscle cell (VSMCs) were incubated with vehicle (methanol) or PUGNAc (100 µM). PUGNAc produced a time-dependent increase in O-GlcNAc levels in VSMC and decreased endothelium-dependent relaxation, which was prevented by apocynin and Tiron, suggesting that •O2- contributes to endothelial dysfunction under augmented O-GlcNAc levels...
August 9, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28754099/antioxidant-and-vasorelaxant-activities-induced-by-northeastern-brazilian-fermented-grape-skins
#17
José George F Albuquerque, Valéria L Assis, Arthur J P O Almeida, Ionaldo J L D Basílio, Melissa N Luciano, Bruno R L A Meireles, Ângela M T M Cordeiro, Islânia G A Araújo, Robson C Veras, Thaís P Ribeiro, Isac A Medeiros
BACKGROUND: In northeastern Brazil, grape pomace has become a potential alternative byproduct because of the recover phenolic compounds from the vinification process. Comparative analyses were performed between lyophilized extract of grape skins from pomace, described as fermented (FGS), and fresh, unfermented (UGS) grape skins to show the relevant brand's composition upon the first maceration in winemaking. METHODS: The use of in vitro testing such as Folin-Ciocalteu's, DPPH free radical scavenger and HPLC methods were performed to evidence antioxidant effect and phenolic compounds...
July 28, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28733475/gper-modulates-tone-and-coronary-vascular-reactivity-in-male-and-female-rats
#18
Angelina Rafaela Debortoli, Wender do Nascimento Rouver, Nathalie Tristão Banhos Delgado, Vinicius Mengal, Erick Roberto Gonçalves Claudio, Laena Pernomian, Lusiane Maria Bendhack, Margareth Ribeiro Moysés, Roger Lyrio Dos Santos
Compared with age-matched men, premenopausal women are largely protected from coronary artery disease, a difference that is lost after menopause. The effects of oestrogens are mediated by the activation of nuclear receptors (ERα and ERβ) and by the G protein-coupled oestrogen receptor (GPER). This study aims to evaluate the potential role of GPER in coronary circulation in female and male rats. The baseline coronary perfusion pressure (CPP) and the concentration-response curve with a GPER agonist (G-1) were evaluated in isolated hearts before and after the blockade of GPER...
August 2017: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/28630072/pseudomonas-quinolone-signal-induces-oxidative-stress-and-inhibits-heme-oxygenase-1-expression-in-lung-epithelial-cells
#19
Maher Y Abdalla, Traci Hoke, Javier Seravalli, Barbara L Switzer, Melissa Bavitz, Jill D Fliege, Peter J Murphy, Bradley E Britigan
Pseudomonasaeruginosa causes lung infections in patients with cystic fibrosis (CF). The Pseudomonas quinolone signal (PQS) compound is a secreted P. aeruginosa virulence factor that contributes to the pathogenicity of P. aeruginosa We were able to detect PQS in sputum samples from CF patients infected with P. aeruginosa but not in samples from uninfected patients. We then tested the hypothesis that PQS induces oxidative stress in host cells by determining the ability of PQS to induce the production of reactive oxygen species (ROS) in lung epithelial cells (A549 and primary normal human bronchial epithelial [NHBE]) cells and macrophages (J774A...
September 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28590009/effect-of-rmnsod-on-sodium-reabsorption-in-renal-proximal-tubule-in-ochratoxin-a-treated-rats
#20
Sara Damiano, Maria Valeria Puzio, Caterina Squillacioti, Nicola Mirabella, Enrica Zona, Aldo Mancini, Antonella Borrelli, Carlo Astarita, Silvia Boffo, Antonio Giordano, Luigi Avallone, Salvatore Florio, Roberto Ciarcia
Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus and Penicillium that represent toxic real threat for human beings and animal health. In this study we evaluated the effect of a new recombinant mitochondrial manganese containing superoxide dismutase (rMnSOD) on oxidative stress and on the alterations of fluid reabsorption in renal proximal tubule (PT) as possible causes of OTA nephrotoxicity. Finally, we have measured the concentration of O2(-) in the kidney through dihydroethidium assay (DHE) and nitric oxide (NO) concentration through nitrites and nitrates assay...
June 7, 2017: Journal of Cellular Biochemistry
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