keyword
MENU ▼
Read by QxMD icon Read
search

dihydroethidium

keyword
https://www.readbyqxmd.com/read/27902770/mitochondrial-targeted-decyl-triphenylphosphonium-enhances-2-deoxy-d-glucose-mediated-oxidative-stress-and-clonogenic-killing-of-multiple-myeloma-cells
#1
Jeanine Schibler, Ann M Tomanek-Chalkley, Jessica L Reedy, Fenghuang Zhan, Douglas R Spitz, Michael K Schultz, Apollina Goel
Therapeutic advances have markedly prolonged overall survival in multiple myeloma (MM) but the disease currently remains incurable. In a panel of MM cell lines (MM.1S, OPM-2, H929, and U266), using CD138 immunophenotyping, side population staining, and stem cell-related gene expression, we demonstrate the presence of stem-like tumor cells. Hypoxic culture conditions further increased CD138low stem-like cells with upregulated expression of OCT4 and NANOG. Compared to MM cells, these stem-like cells maintained lower steady-state pro-oxidant levels with increased uptake of the fluorescent deoxyglucose analog...
2016: PloS One
https://www.readbyqxmd.com/read/27893783/role-of-pten-in-oxidative-stress-and-dna-damage-in-the-liver-of-whole-body-pten-haplodeficient-mice
#2
Ezgi Eyluel Bankoglu, Oliver Tschopp, Johannes Schmitt, Philipp Burkard, Daniel Jahn, Andreas Geier, Helga Stopper
Type 2 diabetes (T2DM) and obesity are frequently associated with non-alcoholic fatty liver disease (NAFLD) and with an elevated cancer incidence. The molecular mechanisms of carcinogenesis in this context are only partially understood. High blood insulin levels are typical in early T2DM and excessive insulin can cause elevated reactive oxygen species (ROS) production and genomic instability. ROS are important for various cellular functions in signaling and host defense. However, elevated ROS formation is thought to be involved in cancer induction...
2016: PloS One
https://www.readbyqxmd.com/read/27878268/crocus-sativus-l-protects-against-sds%C3%A2-induced-intestinal-damage-and-extends-lifespan-in-drosophila-melanogaster
#3
Zonglin Liu, Yuchen Chen, Hong Zhang, Li Hua Jin
Medicinal plants are important sources of potentially therapeutic biochemical drugs. Crocus sativus L. has been used to treat various diseases in China, the Republic of Korea and Japan. The present study investigated the protective effect of C. sativus L. extract in Drosophila melanogaster intestinal immunity. Wild‑type flies were fed standard cornmeal‑yeast medium and used as controls, and flies supplemented with 1% C. sativus L. aqueous extract in standard medium were used as the experimental group...
November 15, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27865407/resveratrol-prevents-capacitation-like-changes-and-improves-in%C3%A2-vitro-fertilizing-capability-of-buffalo-frozen-thawed-sperm
#4
Valentina Longobardi, Gianluigi Zullo, Angela Salzano, Carolina De Canditiis, Andrea Cammarano, Luca De Luise, Maria Valeria Puzio, Gianluca Neglia, Bianca Gasparrini
The aim of this study was to evaluate the effect of resveratrol supplementation of semen extender on fertility parameters of frozen-thawed buffalo sperm. After the initial semen assessment, buffalo semen was cryopreserved in BioXcell containing 0 (control group), 0.5, 1, 10, and 50-μM resveratrol. After thawing, viability, motility, and capacitation status (assessed by localization of phosphotyrosine-containing proteins) were evaluated. Based on the results of the dose-response trial, the concentration of 50 μM was selected for further assessments, such as membrane integrity, total antioxidant capacity, reactive oxygen species, and lipid peroxidation (LPO) levels...
January 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/27863990/cytochrome-p450-enzymes-but-not-nadph-oxidases-are-the-source-of-the-nadph-dependent-lucigenin-chemiluminescence-in-membrane-assays
#5
Flávia Rezende, Kim-Kristin Prior, Oliver Löwe, Ilka Wittig, Valentina Strecker, Franziska Moll, Valeska Helfinger, Frank Schnütgen, Nina Kurrle, Frank Wempe, Maria Walter, Sven Zukunft, Bert Luck, Ingrid Fleming, Norbert Weissmann, Ralf P Brandes, Katrin Schröder
: Measuring NADPH oxidase (Nox)-derived reactive oxygen species (ROS) in living tissues and cells is a constant challenge. All probes available display limitations regarding sensitivity, specificity or demand highly specialized detection techniques. In search for a presumably easy, versatile, sensitive and specific technique, numerous studies have used NADPH-stimulated assays in membrane fractions which have been suggested to reflect Nox activity. However, we previously found an unaltered activity with these assays in triple Nox knockout mouse (Nox1-Nox2-Nox4(-/-)) tissue and cells compared to wild type...
November 15, 2016: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/27859785/increased-monocyte-derived-reactive-oxygen-species-in-type-2-diabetes-role-of-endoplasmic-reticulum-stress
#6
Robert M Restaino, Shekhar H Deo, Alan R Parrish, Paul J Fadel, Jaume Padilla
Recent evidence suggests that exposure of human monocytes to glucolipotoxic media to mimic the composition of plasma of patients with type 2 diabetes (T2D) results in the induction of endoplasmic reticulum stress markers and formation of reactive oxygen species (ROS). The extent to which these findings translate to patients with T2D remains unclear. Thus, we first measured ROS (dihydroethidium fluorescence) in peripheral blood mononuclear cells (PBMCs) from whole blood of T2D patients (n = 8) and compared to age matched healthy controls (n = 8)...
November 18, 2016: Experimental Physiology
https://www.readbyqxmd.com/read/27833552/hydrogen-h2-inhibits-isoproterenol-induced-cardiac-hypertrophy-via-antioxidative-pathways
#7
Yaxing Zhang, Jingting Xu, Zhiyuan Long, Chen Wang, Ling Wang, Peng Sun, Ping Li, Tinghuai Wang
Background and Purpose: Hydrogen (H2) has been shown to have a strong antioxidant effect on preventing oxidative stress-related diseases. The goal of the present study is to determine the pharmacodynamics of H2 in a model of isoproterenol (ISO)-induced cardiac hypertrophy. Methods: Mice (C57BL/6J; 8-10 weeks of age) were randomly assigned to four groups: Control group (n = 10), ISO group (n = 12), ISO plus H2 group (n = 12), and H2 group (n = 12). Mice received H2 (1 ml/100g/day, intraperitoneal injection) for 7 days before ISO (0...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27824157/effect-of-p22phox-depletion-on-sympathetic-regulation-of-blood-pressure-in-shrsp-evaluation-in-a-new-congenic-strain
#8
Hasan M Zahid, Mohammed Zubaerul Ferdaus, Hiroki Ohara, Minoru Isomura, Toru Nabika
Oxidative stress in the rostral ventrolateral medulla (RVLM), a sympathetic center in the brainstem, was implicated in the regulation of sympathetic activity in various hypertensive models including stroke-prone spontaneously hypertensive rats (SHRSP). In this study, we evaluated the role of the NADPH oxidases (NOX) in the blood pressure (BP) regulation in RVLM in SHRSP. The P22PHOX-depleted congenic SHRSP (called SP.MES) was constructed by introducing the mutated p22phox gene of Matsumoto Eosinophilic Shinshu rat...
November 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27821539/endothelial-microparticles-from-acute-coronary-syndrome-patients-induce-premature-coronary-artery-endothelial-cells-ageing-and-thrombogenicity-role-of-the-ang-ii-at1-receptor-nadph-oxidase-mediated-activation-of-mapks-and-pi3-kinase-pathways
#9
Malak Abbas, Laurence Jesel, Cyril Auger, Lamia K Amoura, Nathan Messas, Guillaume Manin, Cordula Rumig, Antonio J León-González, Thais P Ribeiro, Grazielle C Silva, Raghida Abou-Merhi, Eva Hamade, Markus Hecker, Yannick Georg, Nabil Chakfe, Patrick Ohlmann, Valérie B Schini-Kerth, Florence Toti, Olivier Morel
BACKGROUND: -Microparticles (MPs) have emerged as a surrogate marker of endothelial dysfunction and cardiovascular risk. This study examined the potential of MPs from senescent endothelial cells (ECs) or from patients with acute coronary syndrome (ACS) to promote premature ECs ageing and thrombogenicity. METHODS: -Primary porcine coronary ECs were isolated from the left circumflex coronary artery. MPs were prepared from ECs and venous blood from patients with ACS (n=30) and from healthy volunteers (n=6) by sequential centrifugation...
November 7, 2016: Circulation
https://www.readbyqxmd.com/read/27816645/stereospecificity-of-ginsenoside-rg2-epimers-in-the-protective-response-against-uv-b-radiation-induced-oxidative-stress-in-human-epidermal-keratinocytes
#10
Hyun-Ji Kang, Yu-Hua Huang, Hye-Won Lim, Daehyun Shin, Kyounghee Jang, Yoonjin Lee, Kyunghoon Kim, Chang-Jin Lim
Ginseng, referring to the dried roots of Panax ginseng C.A. Meyer, has been known as a famous traditional folkloric medicine in East Asian countries for a long time. In recent years, it has been gaining a worldwide popularity as a dietary herbal supplement. Ginsenosides are bioactive ingredients that are responsible for most pharmacological efficacies of ginseng. Ginsenoside Rg2 (Rg2), one of minor protopanaxatriol (PPT)-type ginsenosides, exists in two epimeric forms, 20(S)-ginsenoside Rg2 [20(S)-Rg2] and 20(R)-ginsenoside Rg2 [20(R)-Rg2]...
October 29, 2016: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/27816504/nadph-oxidase-nox-1-mediates-cigarette-smoke-induced-superoxide-generation-in-rat-vascular-smooth-muscle-cells
#11
Kyung-Hwa Chang, Jung-Min Park, Chang Hoon Lee, Bumseok Kim, Kyung-Chul Choi, Seong-Jin Choi, Kyuhong Lee, Moo-Yeol Lee
Smoking is a well-established risk factor for cardiovascular diseases. Oxidative stress is one of the common etiological factors, and NADPH oxidase (NOX) has been suggested as a potential mediator of oxidative stress. In this study, cigarette smoke (CS)-induced superoxide production was characterized in vascular smooth muscle cells (VSMC). CS was prepared in forms of cigarette smoke extract (CSE) and total particulate matter (TPM). Several molecular probes for reactive oxygen species were trialed, and dihydroethidium (DHE) and WST-1 were chosen for superoxide detection considering the autofluorescence, light absorbance, and peroxidase inhibitory activity of CS...
November 3, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27809355/aliskiren-protects-against-myocardial-ischemia-reperfusion-injury-via-an-endothelial-nitric-oxide-synthase-dependent-manner
#12
Yu Chen, Guoliang Meng, Wenli Bai, Yan Ma, Liping Xie, Naila Altaf, Yanning Qian, Yi Han, Yong Ji
Aliskiren, a direct renin inhibitor, was shown potent ability to attenuate hypertension. Our previous research has found that aliskiren protected against myocardial ischemia-reperfusion (I/R) injury and enhanced phosphorylation of endothelial nitric oxide synthase (eNOS) in spontaneously hypertensive rats. However, whether the cardioprotective effect of aliskiren against myocardial I/R injury was eNOS-dependent is unknown. In the present study, 12-week-old male eNOS knockout (eNOS(-/-) ) and wild-type C57BL/6J mice (WT) were orally administrated with the dose of 50 mg/kg/day of aliskiren...
November 3, 2016: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/27804267/reactive-oxygen-species-and-oxidative-stress-in-obesity-recent-findings-and-empirical-approaches
#13
REVIEW
Fiona McMurray, David A Patten, Mary-Ellen Harper
OBJECTIVE: High levels of reactive oxygen species (ROS) are intricately linked to obesity and associated pathologies, notably insulin resistance and type 2 diabetes. However, ROS are also thought to be important in intracellular signaling, which may paradoxically be required for insulin sensitivity. Many theories have been developed to explain this apparent paradox, which have broadened our understanding of these important small molecules. While many sites for intracellular ROS production have been described, mitochondrial generated ROS remain a major contributor in most cell types...
November 2016: Obesity
https://www.readbyqxmd.com/read/27798888/detection-of-redox-imbalance-in-normal-lymphocytes-with-induced-mitochondrial-dysfunction-epr-study
#14
Ekaterina Georgieva, Zhivko Zhelev, Ichio Aoki, Rumiana Bakalova, Tatsuya Higashi
The present study describes a new approach for direct imaging of redox status in live cells using paramagnetic spin-probes, which allows evaluation of the level of oxidative stress due to overproduction of superoxide. The method is based on redox cycling of cell/mitochondria-penetrating nitroxide radicals (e.g. mito-TEMPO) and their electron-paramagnetic resonance (EPR) contrast, which makes them useful molecular sensors for analysis of redox status and oxidative stress in cells and tissues. Oxidative stress was induced in normal human lymphocytes by treatment with 2-methoxyestradiol and rotenone (ME/Rot) at different concentrations...
October 2016: Anticancer Research
https://www.readbyqxmd.com/read/27782741/apocynin-ameliorates-pressure-overload-induced-cardiac-remodeling-by-inhibiting-oxidative-stress-and-apoptosis
#15
J-J Liu, Y Lu, N-N Ping, X Li, Y-X Lin, C F Li
Oxidative stress plays an important role in pressure overload-induced cardiac remodeling. The purpose of this study was to determine whether apocynin, a nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor, attenuates pressure overload-induced cardiac remodeling in rats. After abdominal aorta constriction, the surviving rats were randomly divided into four groups: sham group, abdominal aorta constriction group, apocynin group, captopril group. Left ventricular pathological changes were studied using Masson's trichrome staining...
October 26, 2016: Physiological Research
https://www.readbyqxmd.com/read/27770804/proteases-and-oxidant-stress-control-organic-dust-induction-of-inflammatory-gene-expression-in-lung-epithelial-cells
#16
Kartiga Natarajan, Koteswara R Gottipati, Kiflu Berhane, Buka Samten, Usha Pendurthi, Vijay Boggaram
BACKGROUND: Persistant inflammatory responses to infectious agents and other components in organic dust underlie lung injury and development of respiratory diseases. Organic dust components responsible for eliciting inflammation and the mechanisms by which they cause lung inflammation are not fully understood. We studied the mechanisms by which protease activities in poultry dust extracts and intracellular oxidant stress induce inflammatory gene expression in A549 and Beas2B lung epithelial cells...
October 22, 2016: Respiratory Research
https://www.readbyqxmd.com/read/27769574/carnitine-supplementation-decreases-capacitation-like-changes-of-frozen-thawed-buffalo-spermatozoa
#17
Valentina Longobardi, Angela Salzano, Giuseppe Campanile, Raffaele Marrone, Francesco Palumbo, Milena Vitiello, Gianluigi Zullo, Bianca Gasparrini
The aim of this study was to evaluate the effect of carnitine supplementation of semen extender on fertility parameters of frozen-thawed buffalo sperm. Buffalo semen was cryopreserved in BioXcell containing 0 (control group), 2.5 and 7.5-mM carnitine. After thawing, viability, motility, membrane integrity and capacitation status (assessed by localization of phosphotyrosine-containing proteins and chlortetracycline, chlortetracycline assay) were evaluated. Furthermore, total antioxidant capacity, reactive oxygen species (ROS) and lipid peroxidation levels, as well as adenosine triphosphate (ATP) content and phospholipids concentration were assessed...
January 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/27754219/os-32-04-acute-blood-pressure-control-by-renal-denervation-improved-prognosis-after-cerebral-infarction-through-reduction-of-oxidative-stress-in-shrsp-rats
#18
Yu Hasegawa, Shokei Kim-Mitsuyama
OBJECTIVE: Although hypertensive patients show significant elevation of blood pressure (BP) in acute phase after cerebral infarction, it is still unknown how it should be managed to carry the best prognosis. To address this issue, we investigated the effect of acute control of elevated BP by bilateral renal denervation (RD) against ischemic stroke in SHRSP rats with 90 minutes transient middle cerebral arterial occlusion (MCAO). DESIGN AND METHOD: Seventy-five male SHRSP rats of 15 weeks of age were assigned to sham-operated (control, n = 15) and MCAO + sham (sham, n = 30), and MCAO + RD (RD, n = 30)...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27754101/os-21-06-three-month-exposure-to-endothelin-1-overexpression-increases-blood-pressure-and-causes-small-artery-injury
#19
Pierre Paradis, Suellen C Coelho, Sofiane Ouerd, Júlio C Fraulob-Aquino, Stefan Offermanns, Ernesto L Schiffrin
OBJECTIVE: The mechanisms of blood pressure (BP) regulation by endothelin (ET)-1 produced by endothelial cells are complex and remain unclear. Recently, we developed a transgenic mouse with tamoxifen-inducible endothelium-restricted human ET-1 overexpression (ieET-1) using Cre/loxP technology. ieET-1 mice exhibited a BP increase after three weeks of induction in an ET type A receptor-dependent manner, in absence of evident vascular injury. It is unknown whether long-term exposure to ET-1 overexpression results in persistent BP elevation and vascular injury...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27754054/os-15-02-matrix-metalloproteinase-2-knockout-protects-from-angiotensin-ii-induced-vascular-injury-in-part-via-inhibition-of-egfr-and-erk1-2-activation
#20
Pierre Paradis, Tlili Barhoumi, Muhammad Oneeb Rehman Mian, Julio C Fraulob-Aquino, Asia Rehman, Nourredine Idris-Khodja, Ernesto L Schiffrin
OBJECTIVE: Matrix metalloproteinase 2 (MMP2) is involved in cardiovascular disease. Whether MMP2 plays a role in hypertension and vascular damage is unknown. We hypothesized that Mmp2 knockout will prevent angiotensin (Ang) II-induced hypertension and vascular injury. DESIGN AND METHOD: Mmp2 knockout (Mmp2) and wild-type (WT) mice were infused with Ang II (1000 ng/kg/min, SC) for 14 days. Systolic blood pressure (SBP) was measured by telemetry, and mesenteric artery (MA) endothelial function and vascular remodeling by pressurized myography...
September 2016: Journal of Hypertension
keyword
keyword
100150
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"