keyword
MENU ▼
Read by QxMD icon Read
search

DHE

keyword
https://www.readbyqxmd.com/read/29470281/vascular-nitric-oxide-superoxide-balance-and-thrombus-formation-after-acute-exercise
#1
Kamil Przyborowski, Bartosz Proniewski, Joanna Czarny, Marta Smeda, Barbara Sitek, Agnieszka Zakrzewska, Jerzy A Zoladz, Stefan Chlopicki
INTRODUCTION: An acute bout of strenuous exercise in humans results in transient impairment of NO-dependent function, but it remains unknown whether this phenomenon is associated with increased risk of post-exercise thrombotic events. This study aimed to evaluate effects of a single bout of exhaustive running in mice on the balance of vascular nitric oxide (NO)/reactive oxygen species (ROS) production, and on thrombogenicity. METHODS: At different time-points (0h, 2h and 4h) after exercise and in sedentary C57BL/6 mice the production of NO and superoxide (O2) in aorta was measured by electron paramagnetic resonance (EPR) spin trapping and by dihydroethidium (DHE)/HPLC-based method, respectively, while collagen-induced thrombus formation was analyzed in a microchip-based flow-chamber system (T-TAS)...
February 21, 2018: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/29423117/oxidative-stress-promotes-exit-from-the-stem-cell-state-and-spontaneous-neuronal-differentiation
#2
Qidong Hu, Puja Khanna, Belinda Shu Ee Wong, Zealyn Shi Lin Heng, Charannya Sozheesvari Subhramanyam, Lal Zo Thanga, Sharon Wui Sing Tan, Gyeong Hun Baeg
Reactive oxygen species (ROS) play important roles in fundamental cellular processes such as proliferation and survival. Here we investigated the effect of oxidative stress on stem cell maintenance and neuronal differentiation in a human embryonic stem cell (hESC) model, Ntera2 (NT2). CM-H2DCFDA and DHE assays confirmed that the oxidizing agent paraquat could induce a high level of ROS in NT2 cells. Quantitative PCR, Western blotting and immunocytochemistry showed that paraquat-induced oxidative stress suppressed the expression of stemness markers, including NANOG, OCT4 and TDGF1, whereas it enhanced the spontaneous expression of neuronal differentiation markers such as PAX6, NEUROD1, HOXA1, NCAM, GFRA1 and TUJ1...
January 9, 2018: Oncotarget
https://www.readbyqxmd.com/read/29399074/protective-effects-of-d-limonene-against-transient-cerebral-ischemia-in-stroke-prone-spontaneously-hypertensive-rats
#3
Xifeng Wang, Gang Li, Wei Shen
Stroke is a leading cause of disability and death world-wide and there is currently a lack of effective treatments for acute stroke. D-Limonene is a common natural monocyclic monoterpene possessing various activities. The present study aimed to evaluate the therapeutic efficacy of D-limonene against ischemia-associated cerebral injury in hypertensive SHRsp rats. Although systolic blood pressure was not altered by ischemia, D-Limonene decreased the systolic blood pressure of SHRsp rats following stroke. Induction of stroke resulted in increased escape latency time, decreased time spent in the target quadrant in the probe trial, decreased capacity to distinguish between familiar objects and novel objects, and increased sensory neglect in the SHRsp rat, however these symptoms were significantly inhibited by D-limonene...
January 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29390203/enos-uncoupling-a-therapeutic-target-for-ischemic-foot-of-diabetic-rat
#4
Ling Gao, Ailin Yu, Jingcheng Liu, Lina Ma, Jing Li
AIM: To determine the relationship between eNOS uncoupling and diabetic ischemic foot and whether reversing eNOS uncoupling by Dihydrofolate reductase (DHFR) transfection or folic acid (FA) supplementation can be beneficiary in diabetic ischemic foot. METHODS: The bilateral common iliac artery of diabetic rats were ligated to establish the diabetic ischemic foot animal model. DHFR transfection was implemented via femoral artery and muscle injection of in vivo transfection reagent mixture (GenEscortIII) every 4 days during the 2 weeks intervention...
February 1, 2018: Experimental and Clinical Endocrinology & Diabetes
https://www.readbyqxmd.com/read/29353158/quantification-of-light-induced-minisog-superoxide-production-using-the-selective-marker-2-hydroxyethidium
#5
Miriam E Barnett, Timothy M Baran, Thomas H Foster, Andrew P Wojtovich
Genetically-encoded photosensitizers produce reactive oxygen species (ROS) in response to light. Transgenic expression of fusion proteins can target the photosensitizers to specific cell regions and permit the spatial and temporal control of ROS production. These ROS-generating proteins (RGPs) are widely used for cell ablation, mutagenesis and chromophore-assisted light inactivation of target proteins. However, the species produced by RGPs are unclear due to indirect measures with confounding interpretations...
January 17, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29322013/dillenia-indica-l-attenuates-diabetic-nephropathy-via-inhibition-of-advanced-glycation-end-products-accumulation-in-stz-nicotinamide-induced-diabetic-rats
#6
Navpreet Kaur, Lalit Kishore, Randhir Singh
The present study was aimed to evaluate advanced glycation end products (AGEs) inhibitory activity of alcohol and hydro-alcohol extract (DAE and DHE) of Dillenia indica L. (Family: Dilleniaceae) and its potential in treatment of diabetic nephropathy by targeting markers of oxidative stress. D. indica was evaluated for its in vitro inhibitory activity against formation of AGEs by using bovine serum albumin. Diabetes was induced in male Wistar rats by streptozotocin (65 mg/kg i.p.) 15 min after nicotinamide (230 mg/kg, i...
January 2018: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/29282820/ergosterol-is-mainly-located-in-the-cytoplasmic-leaflet-of-the-yeast-plasma-membrane
#7
Lukasz M Solanko, David P Sullivan, Yves Y Sere, Maria Szomek, Anita Lunding, Katarzyna A Solanko, Azra Pizovic, Lyubomir D Stanchev, Thomas Günther Pomorski, Anant K Menon, Daniel Wüstner
Transbilayer lipid asymmetry is a fundamental characteristic of the eukaryotic cell plasma membrane (PM). While PM phospholipid asymmetry is well documented, the transbilayer distribution of PM sterols such as mammalian cholesterol and yeast ergosterol is not reliably known. We now report that sterols are asymmetrically distributed across the yeast PM, with the majority (~80%) located in the cytoplasmic leaflet. By exploiting the sterol-auxotrophic hem1Δ yeast strain we obtained cells in which endogenous ergosterol was quantitatively replaced with dehydroergosterol (DHE), a closely related fluorescent sterol that functionally and accurately substitutes for ergosterol in vivo...
December 28, 2017: Traffic
https://www.readbyqxmd.com/read/29278298/egg-white-ovotransferrin-derived-ace-inhibitory-peptide-ameliorates-angiotensin-ii-stimulated-insulin-resistance-in-skeletal-muscle-cells
#8
Myoungjin Son, Catherine B Chan, Jianping Wu
SCOPE: The renin-angiotensin system (RAS) is a major contributor to the development of insulin resistance and its related complications. Egg white ovotransferrin-derived tripeptides, IRW, IQW, or LKP were previously identified as the inhibitors of angiotensin converting enzyme (ACE), a key enzyme in the RAS. This study aimed at determining whether these peptides are effective in improving insulin resistance, and their mechanisms of action, in a rat derived skeletal muscle cell line (L6 cells)...
December 26, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29276033/crystal-structures-of-anti-apoptotic-bfl-1-and-its-complex-with-a-covalent-stapled-peptide-inhibitor
#9
Edward P Harvey, Hyuk-Soo Seo, Rachel M Guerra, Gregory H Bird, Sirano Dhe-Paganon, Loren D Walensky
BCL-2 family proteins are high-priority cancer targets whose structures provide essential blueprints for drug design. Whereas numerous structures of anti-apoptotic BCL-2 protein complexes with α-helical BH3 peptides have been reported, the corresponding panel of apo structures remains incomplete. Here, we report the crystal structure of apo BFL-1 at 1.69-Å resolution, revealing similarities and key differences among unliganded anti-apoptotic proteins. Unlike all other BCL-2 proteins, apo BFL-1 contains a surface-accessible cysteine within its BH3-binding groove, allowing for selective covalent targeting by a NOXA BH3-based stapled peptide inhibitor...
December 6, 2017: Structure
https://www.readbyqxmd.com/read/29250761/medical-treatment-guidelines-for-acute-migraine-attacks
#10
Yung-Chu Hsu, Kao-Chang Lin, Treatment Guideline Subcommittee Of Taiwan Headache Society Taiwan Headache Society
In 2015, the American Headache Society (AHS) amended the treatment guideline of acute migraine based on evidence-based medicine (EBM) that all triptans in any form of preparations, acetaminophen, and non-steroid anti-inflammation drugs-NSAID (aspirin, diclofenac, ibuprofen, naproxen), sumatriptan/naproxen, combined acetaminophen/aspirin/caffeine are considered effective (Level A). Previously effective drugs as prochlorperazine, and dihydroergotamine-DHE (excluded inhaled form) were downrated to probable effective (Level B)...
June 15, 2017: Acta Neurologica Taiwanica
https://www.readbyqxmd.com/read/29250674/in-vivo-confocal-microscopy-quantification-of-reactive-oxygen-species-a-working-model-in-rat-kidney
#11
Adrian Ovidiu Văduva, Cosmin Glămeanu, Romeo Negrea, Mirela Danina Muntean, Alis Liliana Carmen Dema
Oxidative stress is a culprit responsible for the development of acute and chronic kidney diseases. We aimed to establish a working model for the dynamic in vivo assessment of reactive oxygen species (ROS) production in rat kidney. A randomized controlled study was performed in 36 adult male Wistar rats subjected to unilateral urinary obstruction (UUO) via ureteral ligation and compared to SHAM controls. Dihydroethidium (DHE) was injected in the femoral vein and in vivo confocal microscopy was performed in the 2nd, 6th and 10th day, respectively after surgery...
2017: Romanian Journal of Morphology and Embryology, Revue Roumaine de Morphologie et Embryologie
https://www.readbyqxmd.com/read/29232407/temporal-changes-in-cardiac-oxidative-stress-inflammation-and-remodeling-induced-by-exercise-in-hypertension-role-for-local-angiotensin-ii-reduction
#12
Sebastião D Silva, Zaira P Jara, Roseli Peres, Larissa S Lima, Cristóforo Scavone, Augusto C Montezano, Rhian M Touyz, Dulce E Casarini, Lisete C Michelini
Exercise training reduces renin-angiotensin system (RAS) activation, decreases plasma and tissue oxidative stress and inflammation in hypertension. However, the temporal nature of these phenomena in response to exercise is unknown. We sought to determine in spontaneously hypertensive rats (SHR) and age-matched WKY controls the weekly effects of training on blood pressure (BP), plasma and left ventricle (LV) Ang II and Ang-(1-7) content (HPLC), LV oxidative stress (DHE staining), gene and protein expression (qPCR and WB) of pro-inflammatory cytokines, antioxidant enzymes and their consequence on hypertension-induced cardiac remodeling...
2017: PloS One
https://www.readbyqxmd.com/read/29232010/protective-effect-of-hsp27-in-atherosclerosis-and-coronary-heart-disease-by-inhibiting-reactive-oxygen-species
#13
Hongli Zhang, Kaiying Jia, Da Sun, Min Yang
OBJECTIVE: To clarify the mechanism of HSP27 as a diagnostic biomarker in coronary heart disease (CHD) and atherosclerosis (AS). METHOD: Expressions of HSP27 in CHD patients and healthy controls were determined by ELISA, and expressions of HSP27 in aortas of CHD patients and healthy controls were measured by Immunohistochemistry. ROC curve was applied to assess the diagnostic performance of HSP27 in CHD. ApoE-/- mice were included and accordingly grouped. The expressions of HSP27 in AS plaque were measured by qRT-PCR, Immunohistochemistry and Western blot...
December 12, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29206074/a-novel-flow-cytometry-assay-using-dihydroethidium-as-redox-sensitive-probe-reveals-nadph-oxidase-dependent-generation-of-superoxide-anion-in-human-platelets-exposed-to-amyloid-peptide-%C3%AE
#14
Aisha Alsheikh Abubaker, Dina Vara, Ian Eggleston, Ilaria Canobbio, Giordano Pula
Reactive oxygen species (ROS) generation is critical in the regulation of platelets, which has important implications in the modulation of hemostasis and thrombosis. Nonetheless, despite several assays have been described and successfully utilized in the past, the analysis of ROS generation in human platelets remains challenging. Here we show that dihydroethidium (DHE) allows the characterization of redox responses upon platelet activation by physiological and pathological stimuli. In particular, the flow cytometry assay that we describe here allowed us to confirm that thrombin, collagen-related peptide (CRP) and arachidonic acid but not adenosine diphosphate (ADP) stimulate superoxide anion formation in a concentration-dependent manner...
December 5, 2017: Platelets
https://www.readbyqxmd.com/read/29179217/ellagic-acid-reduces-high-glucose-induced-vascular-oxidative-stress-through-erk1-2-nox4-signaling-pathway
#15
Artur Rozentsvit, Kevin Vinokur, Sherin Samuel, Ying Li, A Martin Gerdes, Maria Alicia Carrillo-Sepulveda
BACKGROUND/AIMS: Elevated production of reactive oxygen species (ROS) is linked to endothelial dysfunction and is one of the key contributors to the pathogenesis of diabetic vascular complications. Emerging evidence has indicated that ellagic acid (EA), a polyphenol found in fruits and nuts, possesses numerous biological activities including radical scavenging. However, whether EA exerts a vasculo-protective effect via antioxidant mechanisms in blood vessels exposed to diabetic conditions remains unknown...
November 28, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29176858/sepsis-induced-selective-loss-of-nmda-receptors-modulates-hippocampal-neuropathology-in-surviving-septic-mice
#16
Shuibing Zhang, Xueqin Wang, Sha Ai, Wen Ouyang, Yuan Le, Jianbin Tong
BACKGROUND: Sepsis-induced neuroinflammation plays an important role in sepsis-related brain dysfunction. However, the molecules that are targeted during neuroinflammation resulting from sepsis-induced brain dysfunction remain unclear. Herein, we tried to investigate the expression and roles of NMDA receptor subunits during sepsis-related brain dysfunction. METHODS: Sepsis was induced by cecal ligation and perforation (CLP) or by a single intraperitoneal injection of lipopolysaccharide (LPS, 8 mg/kg) in C57BL/6J mice...
2017: PloS One
https://www.readbyqxmd.com/read/29149089/mnsod-and-cyclin-b1-coordinate-a-mito-checkpoint-during-cell-cycle-response-to-oxidative-stress
#17
Amanda L Kalen, Iman M Ahmad, Maher Y Abdalla, Yunxia Q O'Malley, Prabhat C Goswami, Ehab H Sarsour
Communication between the nucleus and mitochondrion could coordinate many cellular processes. While the mechanisms regulating this communication are not completely understood, we hypothesize that cell cycle checkpoint proteins coordinate the cross-talk between nuclear and mitochondrial functions following oxidative stress. Human normal skin fibroblasts, representative of the G₂-phase, were irradiated with 6 Gy of ionizing radiation and assayed for cyclin B1 translocation, mitochondrial function, reactive oxygen species (ROS) levels, and cytotoxicity...
November 17, 2017: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/29107939/klotho-improves-cardiac-function-by-suppressing-reactive-oxygen-species-ros-mediated-apoptosis-by-modulating-mapks-nrf2-signaling-in-doxorubicin-induced-cardiotoxicity
#18
Huolan Zhu, Yan Gao, Shunming Zhu, Qianwei Cui, Jie Du
BACKGROUND Anthracyclines-induced cardiotoxicity has become one of the major restrictions of their clinical applications. Klotho showed cardioprotective effects. This study aimed to investigate the effects and possible mechanisms of klotho on doxorubicin (DOX)-induced cardiotoxicity. MATERIAL AND METHODS Rats and isolated myocytes were exposed to DOX and treated with exogenous klotho. Specific inhibitors and siRNAs silencing MAPKs were also used to treat the animals and/or myocytes. An invasive hemodynamic method was used to determine cardiac functions...
November 6, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29107684/ethylene-glycol-dimethacrylate-and-diethylene-glycol-dimethacrylate-exhibits-cytotoxic-and-genotoxic-effect-on-human-gingival-fibroblasts-via-induction-of-reactive-oxygen-species
#19
Anna Bielecka-Kowalska, Piotr Czarny, Paulina Wigner, Ewelina Synowiec, Bartosz Kowalski, Marzena Szwed, Renata Krupa, Monika Toma, Malgorzata Drzewiecka, Ireneusz Majsterek, Janusz Szemraj, Tomasz Sliwinski, Michał Kowalski
Although methacrylic acid derivatives in their polymeric form are considered to be safe, insufficient polymerization and the release of monomers due to either mechanical or enzymatical factors can lead to their reaching millimolar concentrations in local tissue. The present study evaluates the effect of two methacrylate monomers - ethylene glycol dimethacrylate (EGDMA) and diethylene glycol dimethacrylate (DEGDMA) - on human gingival fibroblasts (HGFs). Both monomers were found to reduce cells viability in MTT assay, increase apoptosis and cause cell cycle arrest in G1/G0 phase...
October 28, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29104724/protective-effects-of-oral-astaxanthin-nanopowder-against-ultraviolet-induced-photokeratitis-in-mice
#20
Fumiya Harada, Tetsuro Morikawa, Anton Lennikov, Anthony Mukwaya, Mira Schaupper, Osamu Uehara, Rie Takai, Koki Yoshida, Jun Sato, Yukihiro Horie, Hiroyuki Sakaguchi, Ching-Zong Wu, Yoshihiro Abiko, Neil Lagali, Nobuyoshi Kitaichi
Purpose: Astaxanthin (AST) has a strong antioxidant cellular membrane chaperone protective effect. Recently, a water-soluble nanosized AST (nano-AST) form was produced, which is expected to improve the efficacy of oral intake effects. The purpose of this study was to examine whether oral nano-AST has therapeutic effects on UV-induced photokeratitis in mice. Methods: C57BL/6 mice were administered twice with either nano-AST, AST oil, lutein, or bilberry extracts 3 hours before and shortly before UV irradiation (dose: 400 mJ/cm(2))...
2017: Oxidative Medicine and Cellular Longevity
keyword
keyword
100149
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"