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https://www.readbyqxmd.com/read/29665365/dehydroeffusol-inhibits-viability-and-epithelial-mesenchymal-transition-through-the-hedgehog-and-akt-mtor-signaling-pathways-in-neuroblastoma-cells
#1
Kang He, Guoqing Duan, Yanyang Li
Neuroblastoma (NB) is the most predominant extracranial solid tumor of infancy in the world. However, current chemotherapy has limited efficacy for more advanced stages of NB due to acquired chemoresistance or acute toxicity in NB patients. Therefore, effective novel anti-NB drugs are desperately needed. The present study aimed to investigate the effects of dehydroeffusol (DHE), a phenanthrene isolated from J. effuses, on NB cells and its underlying mechanism. The results showed that DHE treatment effectively inhibited NB cell viability in a dose-dependent manner...
April 14, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29657902/inhibitory-effects-of-angiotensin-ii-receptor-blockade-on-human-tenon-fibroblast-migration-and-reactive-oxygen-species-production-in-cell-culture
#2
Duri Kim, Ushasree Pattamatta, Elizabeth Kelly, Paul R Healey, Nicole Carnt, Hans Zoellner, Andrew J R White
Purpose: We investigate the effect of angiotensin receptor blockade on the migration of human Tenon fibroblasts (HTF), using irbesartan, an angiotensin II receptor type 1 (AT1R) blocker (ARB) as a potential antifibrotic agent in glaucoma filtration surgery. Methods: Confluent HTF cultures were scratched with a 1 mL pipette tip and treated with either irbesartan (10, 50, and 100 μg/mL) or angiotensin II (2 μg/mL). The extent of HTF migration up to 30 hours, and cell number and morphology at 72 hours was evaluated...
April 2018: Translational Vision Science & Technology
https://www.readbyqxmd.com/read/29650417/%C3%AE-aminoisobutyric-acid-accelerates-the-proliferation-and-differentiation-of-mc3t3-e1-cells-via-moderate-activation-of-ros-signaling
#3
Xiao-Wen Zhu, Kai Ding, Xiao-Yu Dai, Wei-Qi Ling
BACKGROUD: Osteoporosis is one of the bone-metabolic diseases associated with decreased bone renewal and bone mineral density. β-aminoisobutyric acid (BAIBA), a natural thymine catabolite, can reduce inflammation in skeletal muscle and alleviate hepatic endoplasmic reticulum stress. However, the roles of BAIBA in osteoblast proliferation and differentiation remain largely unknown. METHODS: The cultured MC3T3-E1 cells received various treatments in this study, including BAIBA alone, H2 O2 alone, BAIBA plus N-acetyl-l-cysteine and BAIBA plus apocynin...
April 9, 2018: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/29611065/ros-induced-autophagy-reduces-b16f10-melanoma-cell-proliferative-activity
#4
Gustavo Miranda Pires Santos, Susana C P S Oliveira, Juliana C S Monteiro, Sandra R Fagnani, Fernando Pires Sampaio, Neandder Andrade Correia, Pedro J L Crugeira, Antonio L B Pinheiro
Cancer is a pathology characterized by increased cell progression and/or reduced programmed cell death. Melanoma shows a rapid increase in cell progression and its resistance to chemotherapy is associated with uncontrolled apoptosis and to mechanisms that increase the flow of the drug out of the cell. The objective of this study was to evaluate the effects of photodynamic therapy (PDT) on the cell proliferation and cellular alterations in B16F10 murine melanoma. For that, four experimental groups were evaluated: the control group; laser group (ʎ = 660 ηm, 40 mW, 2...
April 2, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/29592844/anti-oxidative-and-anti-apoptotic-effects-of-acupuncture-role-of-thioredoxin-1-in-the-hippocampus-of-vascular-dementia-rats
#5
Wen Zhu, Xue-Rui Wang, Si-Qi Du, Chao-Qun Yan, Na-Na Yang, Lu-Lu Lin, Guang-Xia Shi, Cun-Zhi Liu
Emerging evidence suggests that acupuncture treatment has anti-oxidative effects that effects cognitive impairment in vascular dementia (VD) rats. In the present study, we aimed to investigate whether thioredoxin-1 (Trx-1)/thioredoxin reductase-1 (TrxR-1) were involved in the beneficial effects of acupuncture. After 2-weeks of acupuncture treatment, Morris water maze (MWM), dihydroethidium (DHE) staining, Nissl staining and TdT-mediated dUTP nick end labeling (TUNEL) staining were used to assess the effects of acupuncture on cognitive function and hippocampal neuronal injury in two-vessel occlusion (2VO) model...
March 25, 2018: Neuroscience
https://www.readbyqxmd.com/read/29580834/niemann-pick-c2-protein-regulates-sterol-transport-between-plasma-membrane-and-late-endosomes-in-human-fibroblasts
#6
Zane Berzina, Lukasz M Solanko, Ahmed S Mehadi, Maria Louise V Jensen, Frederik W Lund, Maciej Modzel, Maria Szomek, Katarzyna A Solanko, Alice Dupont, Gitte Krogh Nielsen, Christian W Heegaard, Christer S Ejsing, Daniel Wüstner
Niemann-Pick disease type C2 is a lipid storage disorder in which mutations in the NPC2 protein cause accumulation of lipoprotein-derived cholesterol in late endosomes and lysosomes (LE/LYSs). Whether cholesterol delivered by other means to NPC2 deficient cells also accumulates in LE/LYSs is currently unknown. We show that the close cholesterol analog dehydroergosterol (DHE), when delivered to the plasma membrane (PM) accumulates in LE/LYSs of human fibroblasts lacking functional NPC2. We measured two different time scales of sterol diffusion; while DHE rich LE/LYSs moved by slow anomalous diffusion in disease cells (D ∼ 4...
March 23, 2018: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/29571735/regional-up-regulation-of-nox2-contributes-to-the-differential-vulnerability-of-outer-hair-cells-to-neomycin
#7
Meihao Qi, Yang Qiu, Xueying Zhou, Keyong Tian, Ke Zhou, Fei Sun, Bo Yue, Fuquan Chen, Dingjun Zha, Jianhua Qiu
In hearing loss induced by aminoglycoside antibiotics, the outer hair cells (OHCs) in the basal turn are always more susceptible than OHCs in the apical turn, while the underlying mechanisms remain unknown. In this study, we reported that NAPDH oxidase 2 (NOX2) played an important role in the OHCs damage preferentially in the basal turn. Normally, NOX2 was evenly expressed in OHCs among different turns, at a relatively low level. However, after neomycin treatment, NOX2 was dominantly induced in OHCs in the basal turn...
March 20, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29563825/improvement-of-skin-conditions-by-ingestion-of-aspergillus-kawachii-koji-extract-containing-14-dehydroergosterol-in-a-randomized-double-blind-controlled-trial
#8
Yoshihiko Sugihara, Shigehito Ikushima, Mika Miyake, Takayoshi Kirisako, Yukihiro Yada, Daisuke Fujiwara
Purpose: The present study examined the effect of ingestion of Koji extract containing 14-dehydroergosterol (14-DHE), prepared from Aspergillus kawachii NBRC4308, on improvement of skin conditions among healthy volunteers. Subjects and methods: In a randomized, double-blind, placebo-controlled, parallel-group study, 70 healthy adult women who felt that their skin was dry ingested either a placebo dietary supplement or Koji extract (200 mg/day) supplement containing 0...
2018: Clinical, Cosmetic and Investigational Dermatology
https://www.readbyqxmd.com/read/29521149/protective-properties-of-geniposide-against-uv-b-induced-photooxidative-stress-in-human-dermal-fibroblasts
#9
Daehyun Shin, Sihyeong Lee, Yu-Hua Huang, Hye-Won Lim, Yoonjin Lee, Kyounghee Jang, Yongwan Cho, Sang Jun Park, Dae-Duk Kim, Chang-Jin Lim
CONTEXT: Geniposide (genipin-1-O-β-d-glucoside) is a major bioactive ingredient in the fruits of gardenia [Gardenia jasminoides J. Ellis (Rubiaceae)], a traditional herbal medicine in Asian countries. OBJECTIVE: This work assesses the skin anti-photoaging potential of geniposide in human dermal fibroblasts under UV-B irradiation. MATERIALS AND METHODS: The anti-photoaging property of geniposide, at varying concentrations (5, 12 and 30 μM) treated for 30 min prior to UV-B irradiation, was evaluated by analysing reactive oxygen species (ROS), promatrix metalloproteinase-2 (proMMP-2), glutathione (GSH), superoxide dismutase (SOD), nuclear factor erythroid 2-related factor 2 (Nrf2) and cellular viability...
December 2018: Pharmaceutical Biology
https://www.readbyqxmd.com/read/29516493/live-cell-imaging-of-new-polyene-sterols-for-improved-analysis-of-intracellular-cholesterol-transport
#10
M Modzel, K A Solanko, M Szomek, S K Hansen, A Dupont, L J Nåbo, J Kongsted, D Wüstner
Analysis of intracellular cholesterol transport by fluorescence microscopy requires suitable fluorescent analogues of cholesterol. Most existing cholesterol analogues contain lipophilic dyes which can compromise the sterol properties in membranes. An alternative strategy is to introduce additional double bonds into the sterol ring system resulting in intrinsic fluorescence, while at the same time keeping the cholesterol-like properties of the analogues. Existing polyene sterols, such as dehydroergosterol (DHE) or cholestatrienol (CTL), however, contain only three double bonds and suffer from low brightness, significant photobleaching and excitation/emission in the ultraviolet region...
March 8, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29507391/functional-trim24-degrader-via-conjugation-of-ineffectual-bromodomain-and-vhl-ligands
#11
Lara N Gechijian, Dennis L Buckley, Matthew A Lawlor, Jaime M Reyes, Joshiawa Paulk, Christopher J Ott, Georg E Winter, Michael A Erb, Thomas G Scott, Mousheng Xu, Hyuk-Soo Seo, Sirano Dhe-Paganon, Nicholas P Kwiatkowski, Jennifer A Perry, Jun Qi, Nathanael S Gray, James E Bradner
The addressable pocket of a protein is often not functionally relevant in disease. This is true for the multidomain, bromodomain-containing transcriptional regulator TRIM24. TRIM24 has been posited as a dependency in numerous cancers, yet potent and selective ligands for the TRIM24 bromodomain do not exert effective anti-proliferative responses. We therefore repositioned these probes as targeting features for heterobifunctional protein degraders. Recruitment of the VHL E3 ubiquitin ligase by dTRIM24 elicits potent and selective degradation of TRIM24...
March 5, 2018: Nature Chemical Biology
https://www.readbyqxmd.com/read/29500566/development-of-an-in-vitro-3d-brain-tissue-model-mimicking-in-vivo-like-pro-inflammatory-and-pro-oxidative-responses
#12
Hyung Joon Cho, Scott S Verbridge, Rafael V Davalos, Yong W Lee
To analyze complex inflammatory responses in an in vitro system, we constructed a new 3D in vitro brain tissue model that exhibits in vivo-like tissue responses (e.g. immune cell phenotypes, and molecular response) to inflammatory stimuli. Finite element modeling of oxygen diffusion and cellular oxygen consumption predicted the oxygen profile within 3D structures, consisting of Type I collagen hydrogel embedded with murine microglia. Viability and cytotoxicity analyses supported the mathematical analysis, determining optimal cell growth conditions for 3D construct development...
March 2, 2018: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/29494287/resveratrol-regulates-blood-pressure-by-enhancing-ampk-signaling-to-downregulate-a-rac1-derived-nadph-oxidase-in-the-central-nervous-system
#13
Tung-Chen Yeh, Ching-Sen Shin, Hsin-Hung Chen, Chi-Cheng Lai, Gwo-Ching Sun, Ching-Jiunn Tseng, Pei-Wen Cheng
Resveratrol is a polyphenol with pleiotropic effects against oxidative damage that has been widely implicated in the lowering of hypertension risk. The purpose of this study was to determine whether improve NO release in the brain either through the activation of AMPK or reduced Rac1-induced ROS generation, thereby reducing BP in rats with fructose-induced hypertension. The rats were fed with 10% fructose or crestor (rosuvastatin; 1.5 mg/kg/day) and resveratrol (10 mg/kg/day) treatment for 1 week, the systolic blood pressure (SBP) of the rats were measured by tail-cuff method...
March 1, 2018: Journal of Applied Physiology
https://www.readbyqxmd.com/read/29490624/curcumin-prevents-strokes-in-stroke-prone-spontaneously-hypertensive-rats-by-improving-vascular-endothelial-function
#14
Cong Lan, Xinjian Chen, Yuxun Zhang, Wei Wang, Wei Eric Wang, Yukai Liu, Yue Cai, Hongmei Ren, Shuo Zheng, Lin Zhou, Chunyu Zeng
BACKGROUND: Antioxidants have shown great promise in stroke prevention. Diarylheptanoids (also known as diphenylheptanoids) are a small class of plant secondary metabolites that possess antioxidant activity greater than that of α-tocopherol. Curcumin is the best known member and is mainly extracted from turmeric. This study aimed to explore whether curcumin has a preventive effect on stroke. METHODS: Stroke-prone spontaneously hypertensive rats (SHRsp) were randomly divided into control group (n = 10) and curcumin group (n = 10), and saline or curcumin (100 mg/kg/day) was administrated daily...
March 1, 2018: BMC Cardiovascular Disorders
https://www.readbyqxmd.com/read/29480496/measurement-of-reactive-oxygen-species-ros-and-mitochondrial-ros-in-ampk-knockout-mice-blood-vessels
#15
Qilong Wang, Ming-Hui Zou
Reactive oxygen species (ROS) are a group of unstable and highly reactive molecules or free radicals typically generated as by-products of cellular processes involving molecular oxygen. In vascular cells, the excessive ROS generation results in the initiation and progression of cardiovascular diseases (CVD). Therefore, a dynamic, robust, and accurate ROS detection method in the blood vessels is essential for pathophysiological research studies of the cardiovascular system.In this chapter, we describe a fluorescence dye-based detection method for assaying superoxide and mitochondrial superoxide in mouse aorta using dihydroethidium (DHE) and MitoSOX...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29477480/dehydroevodiamine-and-hortiamine-alkaloids-from-the-traditional-chinese-herbal-drug-evodia-rutaecarpa-are-i-kr-blockers-with-proarrhythmic-effects-in-vitro-and-in-vivo
#16
Igor Baburin, Rosanne Varkevisser, Anja Schramm, Priyanka Saxena, Stanislav Beyl, Phillip Szkokan, Tobias Linder, Anna Stary-Weinzinger, Marcel A G van der Heyden, Marien Houtman, Hiroki Takanari, Malin Jonsson, Jet H D Beekman, Matthias Hamburger, Marc A Vos, Steffen Hering
Evodiae fructus is a widely used herbal drug in traditional Chinese medicine. Evodia extract was found to inhibit hERG channels. The aim of the current study was to identify hERG inhibitors in Evodia extract and to investigate their potential proarrhythmic effects. Dehydroevodiamine (DHE) and hortiamine were identified as IKr (rapid delayed rectifier current) inhibitors in Evodia extract by HPLC-microfractionation and subsequent patch clamp studies on human embryonic kidney cells. DHE and hortiamine inhibited IKr with IC50 s of 253...
February 22, 2018: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29470281/vascular-nitric-oxide-superoxide-balance-and-thrombus-formation-after-acute-exercise
#17
Kamil Przyborowski, Bartosz Proniewski, Joanna Czarny, Marta Smeda, Barbara Sitek, Agnieszka Zakrzewska, Jerzy A Zoladz, Stefan Chlopicki
INTRODUCTION: An acute bout of strenuous exercise in humans results in transient impairment of NO-dependent function, but it remains unknown whether this phenomenon is associated with increased risk of post-exercise thrombotic events. This study aimed to evaluate effects of a single bout of exhaustive running in mice on the balance of vascular nitric oxide (NO)/reactive oxygen species (ROS) production, and on thrombogenicity. METHODS: At different time-points (0h, 2h and 4h) after exercise and in sedentary C57BL/6 mice the production of NO and superoxide (O2) in aorta was measured by electron paramagnetic resonance (EPR) spin trapping and by dihydroethidium (DHE)/HPLC-based method, respectively, while collagen-induced thrombus formation was analyzed in a microchip-based flow-chamber system (T-TAS)...
February 21, 2018: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/29423117/oxidative-stress-promotes-exit-from-the-stem-cell-state-and-spontaneous-neuronal-differentiation
#18
Qidong Hu, Puja Khanna, Belinda Shu Ee Wong, Zealyn Shi Lin Heng, Charannya Sozheesvari Subhramanyam, Lal Zo Thanga, Sharon Wui Sing Tan, Gyeong Hun Baeg
Reactive oxygen species (ROS) play important roles in fundamental cellular processes such as proliferation and survival. Here we investigated the effect of oxidative stress on stem cell maintenance and neuronal differentiation in a human embryonic stem cell (hESC) model, Ntera2 (NT2). CM-H2DCFDA and DHE assays confirmed that the oxidizing agent paraquat could induce a high level of ROS in NT2 cells. Quantitative PCR, Western blotting and immunocytochemistry showed that paraquat-induced oxidative stress suppressed the expression of stemness markers, including NANOG, OCT4 and TDGF1, whereas it enhanced the spontaneous expression of neuronal differentiation markers such as PAX6, NEUROD1, HOXA1, NCAM, GFRA1 and TUJ1...
January 9, 2018: Oncotarget
https://www.readbyqxmd.com/read/29399074/protective-effects-of-d-limonene-against-transient-cerebral-ischemia-in-stroke-prone-spontaneously-hypertensive-rats
#19
Xifeng Wang, Gang Li, Wei Shen
Stroke is a leading cause of disability and death world-wide and there is currently a lack of effective treatments for acute stroke. D-Limonene is a common natural monocyclic monoterpene possessing various activities. The present study aimed to evaluate the therapeutic efficacy of D-limonene against ischemia-associated cerebral injury in hypertensive SHRsp rats. Although systolic blood pressure was not altered by ischemia, D-Limonene decreased the systolic blood pressure of SHRsp rats following stroke. Induction of stroke resulted in increased escape latency time, decreased time spent in the target quadrant in the probe trial, decreased capacity to distinguish between familiar objects and novel objects, and increased sensory neglect in the SHRsp rat, however these symptoms were significantly inhibited by D-limonene...
January 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29390203/enos-uncoupling-a-therapeutic-target-for-ischemic-foot-of-diabetic-rat
#20
Ling Gao, Ailin Yu, Jingcheng Liu, Lina Ma, Jing Li
AIM: To determine the relationship between eNOS uncoupling and diabetic ischemic foot and whether reversing eNOS uncoupling by Dihydrofolate reductase (DHFR) transfection or folic acid (FA) supplementation can be beneficiary in diabetic ischemic foot. METHODS: The bilateral common iliac artery of diabetic rats were ligated to establish the diabetic ischemic foot animal model. DHFR transfection was implemented via femoral artery and muscle injection of in vivo transfection reagent mixture (GenEscortIII) every 4 days during the 2 weeks intervention...
February 1, 2018: Experimental and Clinical Endocrinology & Diabetes
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