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https://www.readbyqxmd.com/read/29206074/a-novel-flow-cytometry-assay-using-dihydroethidium-as-redox-sensitive-probe-reveals-nadph-oxidase-dependent-generation-of-superoxide-anion-in-human-platelets-exposed-to-amyloid-peptide-%C3%AE
#1
Aisha Alsheikh Abubaker, Dina Vara, Ian Eggleston, Ilaria Canobbio, Giordano Pula
Reactive oxygen species (ROS) generation is critical in the regulation of platelets, which has important implications in the modulation of hemostasis and thrombosis. Nonetheless, despite several assays have been described and successfully utilized in the past, the analysis of ROS generation in human platelets remains challenging. Here we show that dihydroethidium (DHE) allows the characterization of redox responses upon platelet activation by physiological and pathological stimuli. In particular, the flow cytometry assay that we describe here allowed us to confirm that thrombin, collagen-related peptide (CRP) and arachidonic acid but not adenosine diphosphate (ADP) stimulate superoxide anion formation in a concentration-dependent manner...
December 5, 2017: Platelets
https://www.readbyqxmd.com/read/29179217/ellagic-acid-reduces-high-glucose-induced-vascular-oxidative-stress-through-erk1-2-nox4-signaling-pathway
#2
Artur Rozentsvit, Kevin Vinokur, Sherin Samuel, Ying Li, A Martin Gerdes, Maria Alicia Carrillo-Sepulveda
BACKGROUND/AIMS: Elevated production of reactive oxygen species (ROS) is linked to endothelial dysfunction and is one of the key contributors to the pathogenesis of diabetic vascular complications. Emerging evidence has indicated that ellagic acid (EA), a polyphenol found in fruits and nuts, possesses numerous biological activities including radical scavenging. However, whether EA exerts a vasculo-protective effect via antioxidant mechanisms in blood vessels exposed to diabetic conditions remains unknown...
November 28, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29176858/sepsis-induced-selective-loss-of-nmda-receptors-modulates-hippocampal-neuropathology-in-surviving-septic-mice
#3
Shuibing Zhang, Xueqin Wang, Sha Ai, Wen Ouyang, Yuan Le, Jianbin Tong
BACKGROUND: Sepsis-induced neuroinflammation plays an important role in sepsis-related brain dysfunction. However, the molecules that are targeted during neuroinflammation resulting from sepsis-induced brain dysfunction remain unclear. Herein, we tried to investigate the expression and roles of NMDA receptor subunits during sepsis-related brain dysfunction. METHODS: Sepsis was induced by cecal ligation and perforation (CLP) or by a single intraperitoneal injection of lipopolysaccharide (LPS, 8 mg/kg) in C57BL/6J mice...
2017: PloS One
https://www.readbyqxmd.com/read/29149089/mnsod-and-cyclin-b1-coordinate-a-mito-checkpoint-during-cell-cycle-response-to-oxidative-stress
#4
Amanda L Kalen, Iman M Ahmad, Maher Y Abdalla, Yunxia Q O'Malley, Prabhat C Goswami, Ehab H Sarsour
Communication between the nucleus and mitochondrion could coordinate many cellular processes. While the mechanisms regulating this communication are not completely understood, we hypothesize that cell cycle checkpoint proteins coordinate the cross-talk between nuclear and mitochondrial functions following oxidative stress. Human normal skin fibroblasts, representative of the G₂-phase, were irradiated with 6 Gy of ionizing radiation and assayed for cyclin B1 translocation, mitochondrial function, reactive oxygen species (ROS) levels, and cytotoxicity...
November 17, 2017: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/29107939/klotho-improves-cardiac-function-by-suppressing-reactive-oxygen-species-ros-mediated-apoptosis-by-modulating-mapks-nrf2-signaling-in-doxorubicin-induced-cardiotoxicity
#5
Huolan Zhu, Yan Gao, Shunming Zhu, Qianwei Cui, Jie Du
BACKGROUND Anthracyclines-induced cardiotoxicity has become one of the major restrictions of their clinical applications. Klotho showed cardioprotective effects. This study aimed to investigate the effects and possible mechanisms of klotho on doxorubicin (DOX)-induced cardiotoxicity. MATERIAL AND METHODS Rats and isolated myocytes were exposed to DOX and treated with exogenous klotho. Specific inhibitors and siRNAs silencing MAPKs were also used to treat the animals and/or myocytes. An invasive hemodynamic method was used to determine cardiac functions...
November 6, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29107684/ethylene-glycol-dimethacrylate-and-diethylene-glycol-dimethacrylate-exhibits-cytotoxic-and-genotoxic-effect-on-human-gingival-fibroblasts-via-induction-of-reactive-oxygen-species
#6
Anna Bielecka-Kowalska, Piotr Czarny, Paulina Wigner, Ewelina Synowiec, Bartosz Kowalski, Marzena Szwed, Renata Krupa, Monika Toma, Malgorzata Drzewiecka, Ireneusz Majsterek, Janusz Szemraj, Tomasz Sliwinski, Michał Kowalski
Although methacrylic acid derivatives in their polymeric form are considered to be safe, insufficient polymerization and the release of monomers due to either mechanical or enzymatical factors can lead to their reaching millimolar concentrations in local tissue. The present study evaluates the effect of two methacrylate monomers - ethylene glycol dimethacrylate (EGDMA) and diethylene glycol dimethacrylate (DEGDMA) - on human gingival fibroblasts (HGFs). Both monomers were found to reduce cells viability in MTT assay, increase apoptosis and cause cell cycle arrest in G1/G0 phase...
October 28, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29104724/protective-effects-of-oral-astaxanthin-nanopowder-against-ultraviolet-induced-photokeratitis-in-mice
#7
Fumiya Harada, Tetsuro Morikawa, Anton Lennikov, Anthony Mukwaya, Mira Schaupper, Osamu Uehara, Rie Takai, Koki Yoshida, Jun Sato, Yukihiro Horie, Hiroyuki Sakaguchi, Ching-Zong Wu, Yoshihiro Abiko, Neil Lagali, Nobuyoshi Kitaichi
Purpose: Astaxanthin (AST) has a strong antioxidant cellular membrane chaperone protective effect. Recently, a water-soluble nanosized AST (nano-AST) form was produced, which is expected to improve the efficacy of oral intake effects. The purpose of this study was to examine whether oral nano-AST has therapeutic effects on UV-induced photokeratitis in mice. Methods: C57BL/6 mice were administered twice with either nano-AST, AST oil, lutein, or bilberry extracts 3 hours before and shortly before UV irradiation (dose: 400 mJ/cm(2))...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29102637/assessment-of-reactive-oxygen-species-generated-by-electronic-cigarettes-using-acellular-and-cellular-approaches
#8
Jiayuan Zhao, Yipei Zhang, Jennifer D Sisler, Justine Shaffer, Stephen S Leonard, Anna M Morris, Yong Qian, Dhimiter Bello, Philip Demokritou
Electronic cigarettes (e-cigs) have fast increased in popularity but the physico-chemical properties and toxicity of the generated emission remain unclear. Reactive oxygen species (ROS) are likely present in e-cig emission and can play an important role in e-cig toxicity. However, e-cig ROS generation is poorly documented. Here, we generated e-cig exposures using a recently developed versatile exposure platform and performed systematic ROS characterization on e-cig emissions using complementary acellular and cellular techniques: 1) a novel acellular Trolox-based mass spectrometry method for total ROS and hydrogen peroxide (H2O2) detection, 2) electron spin resonance (ESR) for hydroxyl radical detection in an acellular and cellular systems and 3) in vitro ROS detection in small airway epithelial cells (SAEC) using the dihydroethidium (DHE) assay...
October 29, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29091901/resistance-training-and-hawthorn-extract-ameliorate-cognitive-deficits-in-streptozotocin-induced-diabetic-rats
#9
Ebrahim Zarrinkalam, Kamal Ranjbar, Iraj Salehi, Nejat Kheiripour, Alireza Komaki
It has been shown that diabetic rats display cognitive impairment. The aim of this study was to investigate the effects of resistance training and natural antioxidants on learning and memory in type 1 diabetic rats. For this purpose, fifty male Wistar rats were randomly divided into five groups: (i) Control (Con, n=10), (ii) Diabetic (D, n=10), (iii) Diabetic+Resistance training (DRT, n=10), (iv) Diabetic+natural antioxidants (DHE, n=10), and (v) Diabetic+Resistance training+ natural antioxidants (DRH, n=10)...
October 28, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29056421/structure-guided-development-of-a-potent-and-selective-non-covalent-active-site-inhibitor-of-usp7
#10
Ilaria Lamberto, Xiaoxi Liu, Hyuk-Soo Seo, Nathan J Schauer, Roxana E Iacob, Wanyi Hu, Deepika Das, Tatiana Mikhailova, Ellen L Weisberg, John R Engen, Kenneth C Anderson, Dharminder Chauhan, Sirano Dhe-Paganon, Sara J Buhrlage
Deubiquitinating enzymes (DUBs) have garnered significant attention as drug targets in the last 5-10 years. The excitement stems in large part from the powerful ability of DUB inhibitors to promote degradation of oncogenic proteins, especially proteins that are challenging to directly target but which are stabilized by DUB family members. Highly optimized and well-characterized DUB inhibitors have thus become highly sought after tools. Most reported DUB inhibitors, however, are polypharmacological agents possessing weak (micromolar) potency toward their primary target, limiting their utility in target validation and mechanism studies...
September 28, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/29051065/dihydroergotamine-mesylate-loaded-dissolving-microneedle-patch-made-of-polyvinylpyrrolidone-for-management-of-acute-migraine-therapy
#11
Cetin Tas, Jessica C Joyce, Hiep X Nguyen, Padmanabhan Eangoor, Jennifer S Knaack, Ajay K Banga, Mark R Prausnitz
Migraine is a widespread neurological disease with negative effects on quality of life and productivity. Moderate to severe acute migraine attacks can be treated with dihydroergotamine mesylate (DHE), an ergot derivative that is especially effective in non-responders to triptan derivatives. To overcome limitations of current DHE formulations in subcutaneous injection and nasal spray such as pain, adverse side effects and poor bioavailability, a new approach is needed for DHE delivery enabling painless self-administration, quick onset of action, and high bioavailability...
October 16, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29022279/the-role-of-%C3%AE-1-and-%C3%AE-2-adrenoceptor-subtypes-in-the-vasopressor-responses-induced-by-dihydroergotamine-in-ritanserin-pretreated-pithed-rats
#12
Eduardo Rivera-Mancilla, Victor H Avilés-Rosas, Guadalupe Manrique-Maldonado, Alain H Altamirano-Espinoza, Belinda Villanueva-Castillo, Antoinette MaassenVanDenBrink, Carlos M Villalón
BACKGROUND: Dihydroergotamine (DHE) is an acute antimigraine agent that displays affinity for dopamine D2-like receptors, serotonin 5-HT1/2 receptors and α1/α2-adrenoceptors. Since activation of vascular α1/α2-adrenoceptors results in systemic vasopressor responses, the purpose of this study was to investigate the specific role of α1- and α2-adrenoceptors mediating DHE-induced vasopressor responses using several antagonists for these receptors. METHODS: For this purpose, 135 male Wistar rats were pithed and divided into 35 control and 100 pretreated i...
October 11, 2017: Journal of Headache and Pain
https://www.readbyqxmd.com/read/28989026/activation-of-nuclear-%C3%AE-catenin-c-myc-axis-promotes-oxidative-stress-injury-in-streptozotocin-induced-diabetic-cardiomyopathy
#13
Peng Liu, Jianfang Su, Xixi Song, Shixiao Wang
Myocardial oxidative stress injury plays a crucial role in the pathogenesis of diabetic cardiomyopathy (DCM). Wnt/β-catenin signaling has been reported to involve in various heart diseases. However, the underlying mechanism associated with β-catenin in DCM remains elusive. This study intended to explore the effect of β-catenin on oxidative damage of DCM by establishing streptozotocin (STZ)-induced diabetic mouse model and hydrogen peroxide (H2O2)-treated myocardial cell model. Cardiac oxidative stress in DCM was detected by measurements of lipid peroxidation and anti-oxidative enzyme activities as well as DHE staining...
December 2, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28967922/inhibition-of-usp10-induces-degradation-of-oncogenic-flt3
#14
Ellen L Weisberg, Nathan J Schauer, Jing Yang, Ilaria Lamberto, Laura Doherty, Shruti Bhatt, Atsushi Nonami, Chengcheng Meng, Anthony Letai, Renee Wright, Hong Tiv, Prafulla C Gokhale, Maria Stella Ritorto, Virginia De Cesare, Matthias Trost, Alexandra Christodoulou, Amanda Christie, David M Weinstock, Sophia Adamia, Richard Stone, Dharminder Chauhan, Kenneth C Anderson, Hyuk-Soo Seo, Sirano Dhe-Paganon, Martin Sattler, Nathanael S Gray, James D Griffin, Sara J Buhrlage
Oncogenic forms of the kinase FLT3 are important therapeutic targets in acute myeloid leukemia (AML); however, clinical responses to small-molecule kinase inhibitors are short-lived as a result of the rapid emergence of resistance due to point mutations or compensatory increases in FLT3 expression. We sought to develop a complementary pharmacological approach whereby proteasome-mediated FLT3 degradation could be promoted by inhibitors of the deubiquitinating enzymes (DUBs) responsible for cleaving ubiquitin from FLT3...
December 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/28961281/chronic-administration-of-mitochondrion-targeted-peptide-ss-31-prevents-atherosclerotic-development-in-apoe-knockout-mice-fed-western-diet
#15
Meng Zhang, Hongting Zhao, Jing Cai, Huihui Li, Qi Wu, Tong Qiao, Kuanyu Li
BACKGROUND: Oxidative stress and inflammatory factors are deeply involved in progression of atherosclerosis. Mitochondrion-targeted peptide SS-31, selectively targeting to mitochondrial inner membrane reacting with cardiolipin, has been reported to inhibit ROS generation and mitigate inflammation. The present study was designed to investigate whether SS-31 could suppress the development of atherosclerosis in vivo. METHODS: Male ApoE-/- mice (8 weeks old) fed with Western diet were treated with normal saline or SS-31 (1 mg/kg/d or 3 mg/kg/d) through subcutaneous injection for 12 weeks...
2017: PloS One
https://www.readbyqxmd.com/read/28931831/hyperhomocysteinemia-alters-retinal-endothelial-cells-barrier-function-and-angiogenic-potential-via-activation-of-oxidative-stress
#16
Riyaz Mohamed, Isha Sharma, Ahmed S Ibrahim, Heba Saleh, Nehal M Elsherbiny, Sadanand Fulzele, Khaled Elmasry, Sylvia B Smith, Mohamed Al-Shabrawey, Amany Tawfik
Hyperhomocysteinemia (HHcy) is associated with several human visual disorders, such as diabetic retinopathy (DR) and age-related macular degeneration (AMD). Breakdown of the blood-retinal barrier (BRB) is linked to vision loss in DR and AMD. Our previous work revealed that HHcy altered BRB in retinal endothelial cells in vivo. Here we hypothesize that homocysteine (Hcy) alters retinal endothelial cell barrier function and angiogenic potential via activation of oxidative stress. Human retinal endothelial cells (HRECs) treated with and without different concentrations of Hcy showed a reduction of tight junction protein expression, increased FITC dextran leakage, decreased transcellular electrical resistance and increased angiogenic potential...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28926338/melk-is-not-necessary-for-the-proliferation-of-basal-like-breast-cancer-cells
#17
Hai-Tsang Huang, Hyuk-Soo Seo, Tinghu Zhang, Yubao Wang, Baishan Jiang, Qing Li, Dennis L Buckley, Behnam Nabet, Justin M Roberts, Joshiawa Paulk, Shiva Dastjerdi, Georg E Winter, Hilary McLauchlan, Jennifer Moran, James E Bradner, Michael J Eck, Sirano Dhe-Paganon, Jean J Zhao, Nathanael S Gray
Thorough preclinical target validation is essential for the success of drug discovery efforts. In this study, we combined chemical and genetic perturbants, including the development of a novel selective maternal embryonic leucine zipper kinase (MELK) inhibitor HTH-01-091, CRISPR/Cas9-mediated MELK knockout, a novel chemical-induced protein degradation strategy, RNA interference and CRISPR interference to validate MELK as a therapeutic target in basal-like breast cancers (BBC). In common culture conditions, we found that small molecule inhibition, genetic deletion, or acute depletion of MELK did not significantly affect cellular growth...
September 19, 2017: ELife
https://www.readbyqxmd.com/read/28925291/isolation-of-a-novel-compound-mimo2-from-the-methanolic-extract-of-moringa-oleifera-leaves-protective-effects-against-vanadium-induced-cytotoxity
#18
Olumayowa O Igado, Jan Glaser, Mario Ramos-Tirado, Ezgi Eylül Bankoğlu, Foluso A Atiba, Ulrike Holzgrabe, Helga Stopper, James O Olopade
Moringa oleifera is reported to be a miracle plant, with positive effects on practically every system in the animal body. The methanolic extract of Moringa oleifera leaves was fractionated using liquid-liquid fractionation, column chromatography and preparative high-performance liquid chromatography (HPLC). Bioassay guided fractionation using Ferric Reducing Antioxidant Power (FRAP) was used to determine the fraction with the highest antioxidative power. Chemical structure was elucidated with nuclear magnetic resonance (NMR) spectroscopy...
September 19, 2017: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/28921392/resveratrol-attenuates-high-glucose-induced-endothelial-cell-apoptosis-via-mediation-of-store-operated-calcium-entry
#19
Ting Lu, Dayan Zhou, Pan Gao, Liangyi Si, Qiang Xu
The aim of this study was to evaluate the influence of resveratrol on HG-induced calcium entry in islet microvascular (MS-1) endothelial cells. MS-1 cells were pretreated with resveratrol or 2-APB (an inhibitor of store-operated calcium entry) and then incubated with high glucose. Cell viability was determined using the cell counting kit-8 method. Reactive oxygen species, endothelial apoptosis, and NO production were detected by DHE probe, TUNEL detection, and nitrate reductase assay kit. Protein levels of SOCE were detected by western blotting...
September 18, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28891299/spatial-learning-responses-to-lipopolysaccharide-in-adult-and-aged-rats
#20
Barbara J Kupferschmid, Barbara A Therrien
Sickness behaviors, adaptive responses to infections, include lethargy, depression, reduced eating and grooming, and concentration problems resulting from interactions between the immune and neuroendocrine systems. Detecting these responses is especially critical in the elderly, as the infections that cause them can lead to cognitive impairment. While deficits in spatial learning, a hippocampal-dependent form of learning, are part of the sickness response, directional heading errors (DHEs; an indicator of spatial-learning deficits) and their time trajectories need further examination...
January 1, 2017: Biological Research for Nursing
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