keyword
MENU ▼
Read by QxMD icon Read
search

Twik

keyword
https://www.readbyqxmd.com/read/29122916/task-1-kcnk3-channels-in-the-lung-from-cell-biology-to-clinical-implications
#1
REVIEW
Andrea Olschewski, Emma L Veale, Bence M Nagy, Chandran Nagaraj, Grazyna Kwapiszewska, Fabrice Antigny, Mélanie Lambert, Marc Humbert, Gábor Czirják, Péter Enyedi, Alistair Mathie
TWIK-related acid-sensitive potassium channel 1 (TASK-1 encoded by KCNK3) belongs to the family of two-pore domain potassium channels. This gene subfamily is constitutively active at physiological resting membrane potentials in excitable cells, including smooth muscle cells, and has been particularly linked to the human pulmonary circulation. TASK-1 channels are sensitive to a wide array of physiological and pharmacological mediators that affect their activity such as unsaturated fatty acids, extracellular pH, hypoxia, anaesthetics and intracellular signalling pathways...
November 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/29103993/role-of-twik-related-potassium-channel-1-trek-1-in-chronic-rhinosinusitis
#2
Ha Kyun Kim, Jae Hyeong Kim, Hyun Jung Kim, Tae Hoon Kim, Sang Hag Lee
TREK-1 constitutively expressed in normal sinus mucosa is down-regulated in inflammatory sinus mucosa where it may be involved in defective epithelial and endothelial barrier integrity. These results suggest that TREK-1 activating compounds may be used therapeutically to maintain the epithelial and endothelial barrier integrity in chronic rhinosinusitis.
November 2, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29093631/the-effect-of-mechanical-ventilation-on-task-1-expression-in-the-brain-in-a-rat-model
#3
Buqi Na, Hong Zhang, Guangfa Wang, Li Dai, Guoguang Xia
Background and Objective: TWIK-related acid-sensitive potassium channel 1 (TASK-1) is closely related to respiratory central control and neuronal injury. We investigated the effect of MV on TASK-1's functions and explored the mechanism using a rat model. Methods: Male Sprague-Dawley rats were randomized to three groups: (1) high tidal volume (HVt): MV for four hours with Vt at 10 mL/kg; (2) low Vt (LVt): MV for four hours with Vt at 5 mL/kg; (3) basal (BAS): anesthetized and unventilated animals...
2017: Canadian Respiratory Journal: Journal of the Canadian Thoracic Society
https://www.readbyqxmd.com/read/29042297/trek-1-pathway-mediates-isoflurane-induced-memory-impairment-in-middle-aged-mice
#4
Yanhui Cai, Zhengwu Peng, Haiyun Guo, Feng Wang, Yi Zeng
Post-operative cognitive dysfunction (POCD) has been widely reported, especially in elderly patients. An association between POCD and inhalational anesthetics, such as isoflurane, has been suggested. The TWIK-related K(+) channel-1 (TREK-1) controls several major cellular responses involved in memory formation and is believed to participate in the development of depression, cerebral ischemia and blood-brain barrier dysfunction. However, the specific role of TREK-1 in mediating anesthesia-induced POCD remains unknown...
October 16, 2017: Neurobiology of Learning and Memory
https://www.readbyqxmd.com/read/29016681/heterodimerization-of-two-pore-domain-k-channel-task1-and-talk2-in-living-heterologous-expression-systems
#5
Yoshiaki Suzuki, Kanako Tsutsumi, Tatsuya Miyamoto, Hisao Yamamura, Yuji Imaizumi
Two-pore-domain K+ (K2P) channels sense a wide variety of stimuli such as mechanical stress, inhalational anesthetics, and changes in extracellular pH or temperature. The K2P channel activity forms a background K+ current and, thereby, contributes to resting membrane potentials. Six subfamilies including fifteen subtypes of K2P channels have been identified. Each K2P channel molecule with two pores consists of a homodimer of each subtype. In addition, a few heterodimers mainly within the same subfamilies have been found recently...
2017: PloS One
https://www.readbyqxmd.com/read/28988317/identification-of-critical-amino-acids-in-the-proximal-c-terminal-of-trek-2-k-channel-for-activation-by-acidic-phi-and-atp-dependent-inhibition
#6
Joohan Woo, Young Keul Jun, Yin-Hua Zhang, Joo Hyun Nam, Dong Hoon Shin, Sung Joon Kim
TWIK-related two-pore domain K(+) channels (TREKs) are regulated by intracellular pH (pHi) and Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2). Previously, Glu(306) in proximal C-terminal (pCt) of mouse TREK-1 was identified as the pHi-sensing residue. The direction of PI(4,5)P2 sensitivity is controversial, and we have recently shown that TREKs are inhibited by intracellular ATP via endogenous PI(4,5)P2 formation. Here we investigate the anionic and cationic residues of pCt for the pHi and ATP-sensitivity in human TREK-2 (hTREK-2)...
October 8, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28944872/isoflurane-exposure-regulates-the-cell-viability-and-bdnf-expression-of-astrocytes-via-upregulation-of-trek%C3%A2-1
#7
Cui-Hong Zhou, Ya-Hong Zhang, Fen Xue, Shan-Shan Xue, Yun-Chun Chen, Ting Gu, Zheng-Wu Peng, Hua-Ning Wang
Neonatal isoflurane exposure in rodents disrupts hippocampal cognitive functions, including learning and memory, and astrocytes may have an important role in this process. However, the molecular mechanisms underlying this disruption are not fully understood. The present study investigated the role of TWIK‑related K+ channel (TREK‑1) in isoflurane‑induced cognitive impairment. Lentiviruses were used to overexpress or knockdown TREK‑1 in astrocytes exposed to increasing concentrations of isoflurane or O2 for 2 h...
November 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944482/molecular-mechanism-for-muscarinic-m1-receptor-mediated-endocytosis-of-twik-related-acid-sensitive-k-1-channels-in-rat-adrenal-medullary-cells
#8
Hidetada Matsuoka, Masumi Inoue
KEY POINTS: The muscarinic acetylcholine receptor (mAChR)-mediated increase in excitability in rat adrenal medullary cells is at least in part due to inhibition of TWIK (tandem of P domains in a weak inwardly rectifying K(+) channel)-related acid-sensitive K(+) (TASK)1 channels. In this study we focused on the molecular mechanism of mAChR-mediated inhibition of TASK1 channels. Exposure to muscarine resulted in a clathrin-dependent endocytosis of TASK1 channels following activation of the muscarinic M1 receptor (M1 R)...
November 15, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28882594/terbinafine-is-a-novel-and-selective-activator-of-the-two-pore-domain-potassium-channel-task3
#9
Paul D Wright, Emma L Veale, David McCoull, David C Tickle, Jonathan M Large, Emma Ococks, Gemma Gothard, Catherine Kettleborough, Alistair Mathie, Jeffrey Jerman
Two-pore domain potassium channels (K2Ps) are characterized by their four transmembrane domain and two-pore topology. They carry background (or leak) potassium current in a variety of cell types. Despite a number of important roles there is currently a lack of pharmacological tools with which to further probe K2P function. We have developed a cell-based thallium flux assay, using baculovirus delivered TASK3 (TWIK-related acid-sensitive K(+) channel 3, KCNK9, K2P9.1) with the aim of identifying novel, selective TASK3 activators...
November 4, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28870160/trek-1-mediates-isoflurane-induced-cytotoxicity-in-astrocytes
#10
Haiyun Guo, Zhengwu Peng, Liu Yang, Xue Liu, Yaning Xie, Yanhui Cai, Lize Xiong, Yi Zeng
BACKGROUND: There are growing concerns that anaesthetic exposure can cause extensive apoptotic degeneration of neurons and the impairment of normal synaptic development and remodelling. However, little attention has been paid to exploring the possible cytotoxicity of inhalation anaesthetics, such as isoflurane, in astrocytes. In this research, we determined that prolonged exposure to an inhalation anaesthetic caused cytotoxicity in astrocytes, and we identified the underlying molecular mechanism responsible for this process...
September 5, 2017: BMC Anesthesiology
https://www.readbyqxmd.com/read/28830434/rational-confederation-of-genes-and-diseases-ngs-interpretation-via-genecards-malacards-and-varelect
#11
Noa Rappaport, Simon Fishilevich, Ron Nudel, Michal Twik, Frida Belinky, Inbar Plaschkes, Tsippi Iny Stein, Dana Cohen, Danit Oz-Levi, Marilyn Safran, Doron Lancet
BACKGROUND: A key challenge in the realm of human disease research is next generation sequencing (NGS) interpretation, whereby identified filtered variant-harboring genes are associated with a patient's disease phenotypes. This necessitates bioinformatics tools linked to comprehensive knowledgebases. The GeneCards suite databases, which include GeneCards (human genes), MalaCards (human diseases) and PathCards (human pathways) together with additional tools, are presented with the focus on MalaCards utility for NGS interpretation as well as for large scale bioinformatic analyses...
August 18, 2017: Biomedical Engineering Online
https://www.readbyqxmd.com/read/28793254/a-soluble-fluorescent-binding-assay-reveals-pip2-antagonism-of-trek-1-channels
#12
Cerrone Cabanos, Miao Wang, Xianlin Han, Scott B Hansen
Lipid regulation of ion channels by low-abundance signaling lipids phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid (PA) has emerged as a central cellular mechanism for controlling ion channels and the excitability of nerves. A lack of robust assays suitable for facile detection of a lipid bound to a channel has hampered the probing of the lipid binding sites and measuring the pharmacology of putative lipid agonists for ion channels. Here, we show a fluorescent PIP2 competition assay for detergent-purified potassium channels, including TWIK-1-related K(+)-channel (TREK-1)...
August 8, 2017: Cell Reports
https://www.readbyqxmd.com/read/28762161/muscarinic-receptors-in-adrenal-chromaffin-cells-physiological-role-and-regulation-of-ion-channels
#13
REVIEW
Masumi Inoue, Hidetada Matsuoka, Keita Harada, Lung-Sen Kao
Adrenal medullary chromaffin cells in mammals are innervated by sympathetic preganglionic nerve fibers, as are sympathetic ganglion neurons. Acetylcholine in the ganglion neurons is well established as mediating fast and slow excitatory postsynaptic potentials through nicotinic and muscarinic acetylcholine receptors (AChRs), respectively. The role of muscarinic AChRs during neuronal transmission in chromaffin cells varies among different mammals. Furthermore, the ion channel mechanisms associated with the muscarinic AChR-mediated increase in excitability of chromaffin cells are complicated and different from the excitation of ganglion neurons, which has been ascribed to the inhibition of M-type K(+) channels...
July 31, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28738160/atrial-selective-k-channel-blockers-potential-antiarrhythmic-drugs-in-atrial-fibrillation
#14
Ursula Ravens
In the wake of demographic change in Western countries, atrial fibrillation has reached an epidemiological scale, yet current strategies for drug treatment of the arrhythmia lack sufficient efficacy and safety. In search of novel medications, atrial-selective drugs that specifically target atrial over other cardiac functions have been developed. Here, I will address drugs acting on potassium (K(+)) channels that are either predominantly expressed in atria or possess electrophysiological properties distinct in atria from ventricles...
November 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28714121/acetylcholine-dependent-upregulation-of-task-1-channels-in-thalamic-interneurons-by-a-smooth-muscle-like-signalling-pathway
#15
Michael Leist, Susanne Rinné, Maia Datunashvili, Ania Aissaoui, Hans-Christian Pape, Niels Decher, Sven G Meuth, Thomas Budde
KEY POINTS: The ascending brainstem transmitter acetylcholine depolarizes thalamocortical relay neurons while it induces hyperpolarization in local circuit inhibitory interneurons. Sustained K(+) currents are modulated in thalamic neurons to control their activity modes; for the interneurons the molecular nature of the underlying ion channels is as yet unknown. Activation of TASK-1 K(+) channels results in hyperpolarization of interneurons and suppression of their action potential firing...
September 1, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28682878/the-involvement-of-potassium-channel-ork1-in-short-term-memory-and-sleep-in-drosophila
#16
Xiaoyan Zhang, Yabin Zheng, Qingguo Ren, Hong Zhou
BACKGROUND: The sleep and cognitive dysfunction are common in major depressive disorders (MDDs). Recently, the 2-pore domain potassium channel twik-related K(+) channel 1 (TREK-1) has been identified to be closely related to the etiology of MDD. However, whether TREK-1 is involved in the regulation of sleep and cognition is still unknown. METHODS: The present study tried to dissect the role of outwardly rectifying K+ channel-1 (ORK1) (TREK-1 homolog in Drosophila) in sleep and cognition in Drosophila...
July 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28676394/the-glycine-hinge-of-transmembrane-segment-2-modulates-the-subcellular-localization-and-gating-properties-in-trek-channels
#17
Ren-Gong Zhuo, Peng Peng, Jian-Quan Zheng, Yun-Long Zhang, Lei Wen, Xiao-Li Wei, Xiao-Yun Ma
TWIK-Related K(+) channels (TREK), including TREK-1 and TREK-2, belong to the TREK/TRAAK subclass of two-pore domain K(+) (K2P) family. The important functions of transmembrane segment 4 (M4)-glycine hinge in TREK channel gating have been characterized, but the roles of M2-hinge (the equivalent residue of M4-hinge) remain unclear. Here, by characterizing the macroscopic currents, subcellular localization and gating properties of their M2-hinge mutants (G166A for TREK-1 and G196A for TREK-2), we investigated the functions of M2-hinge...
August 26, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28605766/genehancer-genome-wide-integration-of-enhancers-and-target-genes-in-genecards
#18
Simon Fishilevich, Ron Nudel, Noa Rappaport, Rotem Hadar, Inbar Plaschkes, Tsippi Iny Stein, Naomi Rosen, Asher Kohn, Michal Twik, Marilyn Safran, Doron Lancet, Dana Cohen
A major challenge in understanding gene regulation is the unequivocal identification of enhancer elements and uncovering their connections to genes. We present GeneHancer, a novel database of human enhancers and their inferred target genes, in the framework of GeneCards. First, we integrated a total of 434 000 reported enhancers from four different genome-wide databases: the Encyclopedia of DNA Elements (ENCODE), the Ensembl regulatory build, the functional annotation of the mammalian genome (FANTOM) project and the VISTA Enhancer Browser...
January 1, 2017: Database: the Journal of Biological Databases and Curation
https://www.readbyqxmd.com/read/28443635/expression-and-localisation-of-two-pore-domain-k2p-background-leak-potassium-ion-channels-in-the-mouse-retina
#19
Steven Hughes, Russell G Foster, Stuart N Peirson, Mark W Hankins
Two-pore domain (K2P) potassium channels perform essential roles in neuronal function. These channels produce background leak type potassium currents that act to regulate resting membrane potential and levels of cellular excitability. 15 different K2P channels have been identified in mammals and these channels perform important roles in a wide number of physiological systems. However, to date there is only limited data available concerning the expression and role of K2P channels in the retina. In this study we conduct the first comprehensive study of K2P channel expression in the retina...
April 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28419410/selective-and-state-dependent-activation-of-tresk-k2p-18-1-background-potassium-channel-by-cloxyquin
#20
Miklós Lengyel, Alice Dobolyi, Gábor Czirják, Péter Enyedi
BACKGROUND AND PURPOSE: Cloxyquin (5-cloroquinolin-8-ol) has been described as an activator of TRESK (K2P 18.1, TWIK-related spinal cord K(+) channel) background potassium channel. We have examined the specificity of the drug by testing several K2P channels. We have investigated the mechanism of cloxyquin-mediated TRESK activation, focusing on the differences between the physiologically relevant regulatory states of the channel. EXPERIMENTAL APPROACH: Potassium currents were measured by two-electrode voltage clamp in Xenopus oocytes and by whole-cell patch clamp in mouse dorsal root ganglion (DRG) neurons...
July 2017: British Journal of Pharmacology
keyword
keyword
100105
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"