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https://www.readbyqxmd.com/read/29143340/k2p-task-2-and-kcnq1-kcne3-k-channels-are-major-players-contributing-to-intestinal-anion-and-fluid-secretion
#1
Francisca Julio-Kalajzić, Sandra Villanueva, Johanna Burgos, Margarita Ojeda, L Pablo Cid, Thomas J Jentsch, Francisco V Sepúlveda
Anion and fluid secretion across the intestinal epithelium, a process altered in cystic fibrosis and secretory diarrhoea, is mediated by cAMP-activated CFTR Cl(-) channels and requires the simultaneous activity of basolateral K(+) channels to maintain cellular ionic homeostasis and membrane potential. This function is fulfilled by the cAMP-activated K(+) channel formed by the association of pore-forming KCNQ1 with its obligatory KCNE3 β-subunit. Studies using mice show sizable cAMP-activated intestinal anion secretion in the absence of either KCNQ1 or KCNE3 suggesting that an alternative K(+) conductance must compensate for the loss of KCNQ1/KCNE3 activity...
November 16, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/29071864/-identification-of-ranae-oviductus-original-animal-based-on-coi-sequences
#2
Meng-Hu Wang, Ting-Guo Kang, Liang Xu, Yan-Yun Yang, Zhen-Jiao Cai
Ranae Oviductus has a high economic and social value, but its adulterants are more numerous, which causes a great confusion to the market. Using DNA bar code technology based on COI sequence for PCR amplification and sequencing of the identified Rana dybowskii, R. chensinensis, R. huanrensis and R. amurensiss, the COI gene database of four species of Rana was established, and comparing the measured sequence with the sequence of GenBank, four kinds of Rana were identified. The MEGA (molecular evolutionary genetics analysis) 7 ...
April 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29058189/studying-mechanosensitivity-of-two-pore-domain-k-channels-in-cellular-and-reconstituted-proteoliposome-membranes
#3
Josefina Del Mármol, Robert A Rietmeijer, Stephen G Brohawn
Mechanical force sensation is fundamental to a wide breadth of biology from the classic senses of touch, pain, hearing, and balance to less conspicuous sensations of proprioception, blood pressure, and osmolarity and basic aspects of cell growth, differentiation, and development. These diverse and essential systems use force-gated (or mechanosensitive) ion channels that convert mechanical stimuli into cellular electrical signals. TRAAK, TREK1, and TREK2 are K(+)-selective ion channels of the two-pore domain K(+) (K2P) family that are mechanosensitive: they are gated open by increasing membrane tension...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29016681/heterodimerization-of-two-pore-domain-k-channel-task1-and-talk2-in-living-heterologous-expression-systems
#4
Yoshiaki Suzuki, Kanako Tsutsumi, Tatsuya Miyamoto, Hisao Yamamura, Yuji Imaizumi
Two-pore-domain K+ (K2P) channels sense a wide variety of stimuli such as mechanical stress, inhalational anesthetics, and changes in extracellular pH or temperature. The K2P channel activity forms a background K+ current and, thereby, contributes to resting membrane potentials. Six subfamilies including fifteen subtypes of K2P channels have been identified. Each K2P channel molecule with two pores consists of a homodimer of each subtype. In addition, a few heterodimers mainly within the same subfamilies have been found recently...
2017: PloS One
https://www.readbyqxmd.com/read/28984357/endocytosis-another-pathway-in-receptor-gq-task-signalling
#5
Donghee Kim
No abstract text is available yet for this article.
November 15, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28937755/stability-and-superconductivity-of-k-p-compounds-under-pressure
#6
Yunxian Liu, Chao Wang, Xiangmu Kong, Defang Duan
We explore the phase diagram, structures, electron properties, and potential superconductivity of the K-P system at pressures of up to 200 GPa through unbiased structure searching techniques and first-principles calculations. Five stable chemical stoichiometries (K4P, K3P, K2P, KP, and KP2) have been theoretically predicted. In particular, P2 units or P-chains are uncovered in K2P, KP, and KP2 compounds with the existence of covalent bonds by analyzing the electron localization functions. And the Bader analysis demonstrates that charges transfer from K atoms to P atoms...
September 22, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28937579/pyrethroids-inhibit-k2p-channels-and-activate-sensory-neurons-basis-of-insecticide-induced-paraesthesias
#7
Aida Castellanos, Alba Andres, Laura Bernal, Gerard Callejo, Nuria Comes, Arcadi Gual, Jonathan P Giblin, Carolina Roza, Xavier Gasull
Pyrethroid insecticides are widely used for pest control in agriculture or in human public health commonly as a topical treatment for scabies and head lice. Exposure to pyrethroids such as permethrin or tetramethrin (TM) causes sensory alterations such as transient pain, burning, stinging sensations, and paraesthesias. Despite the well-known effects of pyrethroids on sodium channels, actions on other channels that control sensory neuron excitability are less studied. Given the role of 2-pore domain potassium (K2P) channels in modulating sensory neuron excitability and firing, both in physiological and pathological conditions, we examined the effect of pyrethroids on K2P channels mainly expressed in sensory neurons...
September 25, 2017: Pain
https://www.readbyqxmd.com/read/28933086/-identification-of-dryopteridis-crassirhizomatis-rhizoma-based-on-psba-trnh-barcode
#8
Zhen-Jiao Cai, Ya-Nan Wu, Liang Xu, Rong Zhao, Bing Wang, Ting-Guo Kang
To identify origin of the medicinal materials Dryopteridis Crassirhizomatis Rhizoma by using the psbA-trnH sequence, the polymerase chain reaction (PCR) amplification and product sequencing of the experimental samples were performed. In order to expand the scope of the study, the psbA-trnH sequences of 8 genera and 3 species were downloaded from GenBank for analysis. DNAMAN 8.0 software was used to show splicing and comparison results of the peak diagrams with analysis of them, and MEGA 6.0 software was to calculate K2P genetic distances and establish clustering tree adjacent genus...
November 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28923939/asymmetric-mechanosensitivity-in-a-eukaryotic-ion-channel
#9
Michael V Clausen, Viwan Jarerattanachat, Elisabeth P Carpenter, Mark S P Sansom, Stephen J Tucker
Living organisms perceive and respond to a diverse range of mechanical stimuli. A variety of mechanosensitive ion channels have evolved to facilitate these responses, but the molecular mechanisms underlying their exquisite sensitivity to different forces within the membrane remains unclear. TREK-2 is a mammalian two-pore domain (K2P) K(+) channel important for mechanosensation, and recent studies have shown how increased membrane tension favors a more expanded conformation of the channel within the membrane...
October 3, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28921904/developing-a-dna-barcode-library-for-perciform-fishes-in-the-south-china-sea-species-identification-accuracy-and-cryptic-diversity
#10
Gang Hou, Wei-Tao Chen, Huo-Sheng Lu, Fei Cheng, Song-Guang Xie
DNA barcodes were studied for 1,353 specimens representing 272 morphological species belonging to 149 genera and 55 families of Perciformes from the South China Sea (SCS). The average Kimura 2-parameter (K2P) distances within species, genera, and families were 0.31%, 8.71%, and 14.52%, respectively. A Neighbor-joining (NJ) tree, Bayesian inference (BI) and maximum-likelihood (ML) trees and Automatic Barcode Gap Discovery (ABGD) revealed 260, 253 and 259 single-species-representing clusters, respectively. Barcoding Gap Analysis (BGA) demonstrated that barcode gaps were present for 178 of 187 species analysed with multiple specimens (95...
September 15, 2017: Molecular Ecology Resources
https://www.readbyqxmd.com/read/28905379/a-new-species-of-pomatoschistus-teleostei-gobiidae-the%C3%A2-mediterranean-s-smallest-marine-fish
#11
S Engin, D Seyhan
The new sand goby species Pomatoschistus nanus (Teleostei: Gobiidae) is described from the northern coast of the Levantine Sea (eastern Mediterranean Sea) based on both morphological and DNA barcoding data. The new species is the smallest fish in the Mediterranean Sea and may be distinguished from congeners by the following features: predorsal area, first dorsal-fin base and breast naked; δ-pore missing; anterior point of the suborbital row b not reaching level of posterior point of suborbital row d; slightly emarginated caudal fin and nape coloration pattern...
September 14, 2017: Journal of Fish Biology
https://www.readbyqxmd.com/read/28882594/terbinafine-is-a-novel-and-selective-activator-of-the-two-pore-domain-potassium-channel-task3
#12
Paul D Wright, Emma L Veale, David McCoull, David C Tickle, Jonathan M Large, Emma Ococks, Gemma Gothard, Catherine Kettleborough, Alistair Mathie, Jeffrey Jerman
Two-pore domain potassium channels (K2Ps) are characterized by their four transmembrane domain and two-pore topology. They carry background (or leak) potassium current in a variety of cell types. Despite a number of important roles there is currently a lack of pharmacological tools with which to further probe K2P function. We have developed a cell-based thallium flux assay, using baculovirus delivered TASK3 (TWIK-related acid-sensitive K(+) channel 3, KCNK9, K2P9.1) with the aim of identifying novel, selective TASK3 activators...
November 4, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28882174/molecular-phylogeny-of-anopheles-hyrcanus-group-members-based-on-its2-rdna
#13
Yuan Fang, Wen-Qi Shi, Yi Zhang
BACKGROUND: The Anopheles hyrcanus group includes 25 species, and is widely distributed in the Oriental and Palaearctic regions. Several species within this group are vectors of malaria, lymphatic filariasis and Japanese encephalitis. It is difficult or impossible to identify cryptic species based on their morphological characteristics, with some closely related species of the Hyrcanus Group have similar adult morphological characteristics. Thus, their molecular identification has been an important complementary method to traditional morphological taxonomy...
September 7, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28851868/an-allosteric-ligand-binding-site-in-the-extracellular-cap-of-k2p-channels
#14
Qichao Luo, Liping Chen, Xi Cheng, Yuqin Ma, Xiaona Li, Bing Zhang, Li Li, Shilei Zhang, Fei Guo, Yang Li, Huaiyu Yang
Two-pore domain potassium (K2P) channels generate leak currents that are responsible for the maintenance of the resting membrane potential, and they are thus potential drug targets for treating diseases. Here, we identify N-(4-cholorphenyl)-N-(2-(3,4-dihydrosioquinolin-2(1H)-yl)-2-oxoethyl)methanesulfonamide (TKDC) as an inhibitor of the TREK subfamily, including TREK-1, TREK-2 and TRAAK channels. Using TKDC as a chemical probe, a study combining computations, mutagenesis and electrophysiology reveals a K2P allosteric ligand-binding site located in the extracellular cap of the channels...
August 29, 2017: Nature Communications
https://www.readbyqxmd.com/read/28839101/hyperoxia-treatment-of-trek-1-trek-2-traak-deficient-mice-is-associated-with-a-reduction-in-surfactant-proteins
#15
Andreas Schwingshackl, Benjamin Lopez, Bin Teng, Charlean L Luellen, Florian Lesage, John Belperio, Riccardo Olcese, Christopher M Waters
We previously proposed a role for the 2-pore domain potassium (K2P) channel TREK-1 in hyperoxia (HO)-induced lung injury. To determine whether redundancy between the 3 TREK isoforms (TREK-1, TREK-2, TRAAK) could protect from HO-induced injury, we now examined the effect of deletion of all 3 TREK isoforms in a clinically relevant scenario of prolonged HO exposure and mechanical ventilation (MV). We exposed WT and TREK-1/TREK-2/TRAAK-deficient (triple ko) mice to either room air, 72 hours HO, MV (high and low tidal volume), or a combination of HO+MV, and measured quasi-static lung compliance, BAL protein concentration, histologic lung injury scores (LIS), cellular apoptosis, and cytokine levels...
August 24, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28769640/molecular-and-morphological-identification-of-biomphalaria-species-from-the-state-of-s%C3%A3-o-paulo-brazil
#16
Raquel Gardini Sanches Palasio, Marisa Cristina de Almeida Guimarães, Fernanda Pires Ohlweiler, Roseli Tuan
DNA barcoding and morphological characters were used to identify adult snails belonging to the genus Biomphalaria from 17 municipalities in the state of São Paulo, Brazil. The DNA barcode analysis also included twenty-nine sequences retrieved from GenBank. The final data set of 104 sequences of the mitochondrial cytochrome oxidase I (COI) gene was analyzed for K2P intraspecific and interspecific divergences, through tree-reconstruction methods (Neighbor-Joining, Maximum Likelihood and Bayesian inference), and by applying different models (ABGD, bPTP, GMYC) to partition the sequences according to the pattern of genetic variation...
2017: ZooKeys
https://www.readbyqxmd.com/read/28743954/molecular-diversity-of-benthic-ctenophores-coeloplanidae
#17
Ada Alamaru, Bert W Hoeksema, Sancia E T van der Meij, Dorothée Huchon
Coeloplanidae, the largest family of benthic ctenophores, comprises 33 species, all described based on traditional morphological characteristics, such as coloration, length, and number of aboral papillae, which are highly variable and can be affected by fixation methods and environmental conditions. Thus, there is a need for reliable genetic markers to complement the morphological identifications at the species level. Here, we analyzed 95 specimens from 11 morphologically distinct species of benthic ctenophores from the Red Sea and Sulu Sea, and tested selected regions of four genetic markers (ITS1, 18S rRNA, 28S rRNA and COI) for their ability to differentiate between species...
July 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28738160/atrial-selective-k-channel-blockers-potential-antiarrhythmic-drugs-in-atrial-fibrillation
#18
Ursula Ravens
In the wake of demographic change in Western countries, atrial fibrillation has reached an epidemiological scale, yet current strategies for drug treatment of the arrhythmia lack sufficient efficacy and safety. In search of novel medications, atrial-selective drugs that specifically target atrial over other cardiac functions have been developed. Here, I will address drugs acting on potassium (K(+)) channels that are either predominantly expressed in atria or possess electrophysiological properties distinct in atria from ventricles...
November 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28728824/origin-of-the-force-the-force-from-lipids-principle-applied-to-piezo-channels
#19
C D Cox, N Bavi, B Martinac
Piezo channels are a ubiquitously expressed, principal type of molecular force sensor in eukaryotes. They enable cells to decode a myriad of physical stimuli and are essential components of numerous mechanosensory processes. Central to their physiological role is the ability to change conformation in response to mechanical force. Here we discuss the evolutionary origin of Piezo in relation to other MS channels in addition to the force that gates Piezo channels. In particular, we discuss whether Piezo channels are inherently mechanosensitive in accordance with the force-from-lipid paradigm which has been firmly established for bacterial MS channels and two-pore domain K(+) (K2P) channels...
2017: Current Topics in Membranes
https://www.readbyqxmd.com/read/28728818/mechanosensitive-piezo-channels-in-the-gastrointestinal-tract
#20
C Alcaino, G Farrugia, A Beyder
Sensation of mechanical forces is critical for normal function of the gastrointestinal (GI) tract and abnormalities in mechanosensation are linked to GI pathologies. In the GI tract there are several mechanosensitive cell types-epithelial enterochromaffin cells, intrinsic and extrinsic enteric neurons, smooth muscle cells and interstitial cells of Cajal. These cells use mechanosensitive ion channels that respond to mechanical forces by altering transmembrane ionic currents in a process called mechanoelectrical coupling...
2017: Current Topics in Membranes
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