Jöran Lücke, Baris Mercanoglu, Tao Zhang, Dimitra E Zazara, Ehud Zigmond, Philipp Seeger, Oliver Mann, Jakob R Izbicki, Thilo Hackert, Samuel Huber, Anastasios D Giannou
To investigate underlying mechanisms for cancer metastasis and promising therapies in animal models, spontaneous metastasis models can be used to recreate metastasis development. Here, we present three mouse models of spontaneous lung and/or liver metastasis induction. We describe steps for cancer cell preparation, mouse analgesia, and three injection techniques (subcutaneous, intracecal, and intramucosal). We then detail procedures for evaluating metastasis. Most of these models generate metastasis in a time span of 4 weeks in the majority of injected mice...
January 17, 2024: STAR protocols